Rh7AG480800

methyltransferase At1g78140, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007867
Physical Location & Seq
Forward (+)
67465545 .. 67470430
4886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG480800.1

Sequence Viewer

Length: 1035 bp
ATGGCGACGACGACCCTCGTCAGCAACCTCTCTCTCCCGAGTCAACTCGGCAACTCGAGGCCCGACTCGCTCAAGCCCAACCCTTTCGCCGTTAATTTCAAACGCTCTTTCGCGGCAATCACTGTTCGAGCTTCTTCCACCGCCTTCGTCGAGGCCACTCCAACTGACCCAGTTGAGGTTCAGGATAATGAGGCCACCACCTGCAACGACTTGTTTGCTTGTCCTATATGCTTTGATCCATTTTCATCAAATGGTGAGTCTGGTTCTGGGTCAGGTTTTGAATGTAGCACTTGTAAGAAGACTTACTTTGGCAATGAGATACATTTGGACCTGACAGTGGCGAGCGGTGCCAAGAACTATGGTGAATCGATGCCGGCTGCCACAGAGATTTTCAGGACTCCGTTGGTATCTTTCCTCTATGAGAGGGGCTGGCGACAAAGCTTTTCGGTATGGGGCGGTTTTCCTGGCCCAGAGAAAGAGTTTGAGTTGATTAAGGATTACTTAAAACCAGTCTTGGGTGGTAGCATCATTGATGCTAGTTGTGGGAGTGGGTTATTTTCTAGACTTTTCGCCAAGAGTGGATTGTTTTCTCATGTTGTTGCTCTTGACTACTCAGAAAATATGTTGAAGCAGTGCTATGAGTTCATTGAAAAGGAAGAGAACTTCCCCAAAGAGAACATAACTTTGGTCAGAGCTGATATTTCTAGGCTTCCCTTTGCTACAAGTTCTGTTGATGCTGTGCATGCTGGTGCTGCGATACACTGTTGGCCTTCACCATCAGGAGCTGTTGCTGAAATTAGCAGAATACTGCGACCTGGTGGGGTGTTTGTTGCGACAACTTACATACTTGATGGTCCTTTTTCCTTTGTCCCATTTTTGAGGGAAGGAACCAAGAGAACAAGGCAAATATCGGGCAGCCAAATCTTCCTATCTGAAGGTGAATTAGAAGACATCTGTACGGCATGTGGGCTAGTAGGTTTTACAGCCGTGAGGAATCGGTTATTTGTGATGATCTCTGCCACAAAACCAAGTTAA

Protein Analysis

344

Amino Acids

37.29

Weight (kDa)

5.4

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 167 - 278 9.8e-10 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 170 - 281 4.6e-08 Methyltransferase domain
Methyltransf_31 PF13847 174 - 281 1.5e-15 Methyltransferase domain
Methyltransf_25 PF13649 176 - 274 2.5e-18 Methyltransferase domain
Methyltransf_11 PF08241 178 - 277 2.3e-22 Methyltransferase domain
Methyltransf_12 PF08242 178 - 276 1.2e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 347
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 1 cut(s) 113
AciI CCGC 4 cut(s) 113, 141, 345, 456
AclWI GGATC 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 954
AfaI GTAC 1 cut(s) 958
AfiI CCNNNNNNNGG 3 cut(s) 175, 337, 515
AgsI TTSAA 4 cut(s) 100, 281, 628, 650
AjnI CCWGG 2 cut(s) 463, 814
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 4 cut(s) 131, 441, 695, 785
AluI AGCT 4 cut(s) 131, 441, 695, 785
AlwI GGATC 1 cut(s) 230
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 2 cut(s) 37, 55
AoxI GGCC 5 cut(s) 59, 153, 192, 466, 767
ApeKI GCWGC 3 cut(s) 377, 752, 915
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspS9I GGNCC 4 cut(s) 60, 328, 467, 854
AsuHPI GGTGA 4 cut(s) 266, 374, 765, 950
AvaI CYCGRG 2 cut(s) 37, 55
AvaII GGWCC 2 cut(s) 328, 854
BanI GGYRCC 1 cut(s) 347
BbsI GAAGAC 2 cut(s) 305, 954
BbvI GCAGC 3 cut(s) 364, 739, 927
BccI CCATC 2 cut(s) 784, 845
BceAI ACGGC 3 cut(s) 74, 971, 975
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 2 cut(s) 465, 816
BfaI CTAG 4 cut(s) 537, 561, 705, 971
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 4 cut(s) 114, 378, 753, 916
BlsI GCNGC 4 cut(s) 115, 379, 754, 917
Bme1390I CCNGG 2 cut(s) 465, 816
Bme18I GGWCC 2 cut(s) 328, 854
BmeT110I CYCGRG 2 cut(s) 37, 55
BmgT120I GGNCC 4 cut(s) 60, 328, 467, 854
BmiI GGNNCC 2 cut(s) 349, 889
BmrFI CCNGG 2 cut(s) 465, 816
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 4 cut(s) 360, 523, 534, 724
BmuI ACTGGG 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 305, 954
BpuEI CTTGAG 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 368
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 337, 515
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 2 cut(s) 170, 509
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 2 cut(s) 465, 816
BseCI ATCGAT 1 cut(s) 368
BseLI CCNNNNNNNGG 3 cut(s) 175, 337, 515
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 170, 509
BseXI GCAGC 3 cut(s) 364, 739, 927
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 5 cut(s) 61, 155, 194, 468, 769
BshNI GGYRCC 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 368
BsiHKCI CYCGRG 2 cut(s) 37, 55
BsiSI CCGG 1 cut(s) 374
BslFI GGGAC 1 cut(s) 854
BslI CCNNNNNNNGG 3 cut(s) 175, 337, 515
BsmFI GGGAC 1 cut(s) 854
BsnI GGCC 5 cut(s) 61, 155, 194, 468, 769
BsoBI CYCGRG 2 cut(s) 37, 55
Bsp143I GATC 2 cut(s) 235, 1011
BspACI CCGC 4 cut(s) 113, 141, 345, 456
BspANI GGCC 5 cut(s) 61, 155, 194, 468, 769
BspCNI CTCAG 1 cut(s) 626
BspDI ATCGAT 1 cut(s) 368
BspFNI CGCG 1 cut(s) 113
BspLI GGNNCC 2 cut(s) 349, 889
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 347
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 2 cut(s) 170, 509
BssAI RCCGGY 1 cut(s) 373
BssMI GATC 2 cut(s) 235, 1011
Bst2UI CCWGG 2 cut(s) 465, 816
Bst4CI ACNGT 3 cut(s) 124, 337, 764
Bst6I CTCTTC 1 cut(s) 651
BstC8I GCNNGC 4 cut(s) 343, 375, 431, 744
BstDEI CTNAG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 2 cut(s) 238, 1014
BstMBI GATC 2 cut(s) 235, 1011
BstMWI GCNNNNNNNGC 4 cut(s) 67, 347, 743, 752
BstNI CCWGG 2 cut(s) 465, 816
BstNSI RCATGY 2 cut(s) 746, 966
BstPAI GACNNNNGTC 1 cut(s) 17
BstSCI CCNGG 2 cut(s) 463, 814
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 364, 739, 927
BstV2I GAAGAC 2 cut(s) 305, 954
Bsu15I ATCGAT 1 cut(s) 368
BsuRI GGCC 5 cut(s) 61, 155, 194, 468, 769
BsuTUI ATCGAT 1 cut(s) 368
BtsI GCAGTG 1 cut(s) 638
BtsIMutI CAGTG 4 cut(s) 120, 342, 638, 760
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 4 cut(s) 343, 375, 431, 744
CaiI CAGNNNCTG 1 cut(s) 785
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 4 cut(s) 60, 328, 467, 854
ClaI ATCGAT 1 cut(s) 368
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 1 cut(s) 957
CviAII CATG 3 cut(s) 593, 743, 963
CviQI GTAC 1 cut(s) 957
DdeI CTNAG 1 cut(s) 613
DpnI GATC 2 cut(s) 237, 1013
DpnII GATC 2 cut(s) 235, 1011
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Eco47I GGWCC 2 cut(s) 328, 854
Eco57I CTGAAG 1 cut(s) 954
Eco88I CYCGRG 2 cut(s) 37, 55
EcoRII CCWGG 2 cut(s) 463, 814
FaeI CATG 3 cut(s) 596, 746, 966
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 485, 517
FaqI GGGAC 1 cut(s) 854
FatI CATG 3 cut(s) 592, 742, 962
Fnu4HI GCNGC 4 cut(s) 114, 378, 753, 916
Fsp4HI GCNGC 4 cut(s) 114, 378, 753, 916
FspBI CTAG 4 cut(s) 537, 561, 705, 971
GluI GCNGC 4 cut(s) 114, 378, 753, 916
HaeIII GGCC 5 cut(s) 61, 155, 194, 468, 769
HapII CCGG 1 cut(s) 374
Hin1II CATG 3 cut(s) 596, 746, 966
HincII GTYRAC 1 cut(s) 44
HindII GTYRAC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 6 cut(s) 40, 65, 257, 365, 397, 994
HpaII CCGG 1 cut(s) 374
HphI GGTGA 4 cut(s) 266, 374, 765, 950
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 3 cut(s) 616, 692, 934
Hpy188III TCNNGA 6 cut(s) 37, 182, 394, 561, 605, 780
Hpy8I GTNNAC 1 cut(s) 44
Hpy99I CGWCG 3 cut(s) 10, 13, 152
HpyAV CCTTC 4 cut(s) 154, 780, 878, 929
HpyCH4III ACNGT 3 cut(s) 124, 337, 764
HpyCH4V TGCA 2 cut(s) 204, 742
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 347, 743, 752
HpyF3I CTNAG 1 cut(s) 613
Hsp92II CATG 3 cut(s) 596, 746, 966
KroI GCCGGC 1 cut(s) 373
KroNI GCCGGC 1 cut(s) 375
Kzo9I GATC 2 cut(s) 235, 1011
LmnI GCTCC 1 cut(s) 782
Lsp1109I GCAGC 3 cut(s) 364, 739, 927
LweI GCATC 4 cut(s) 360, 523, 534, 724
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 4 cut(s) 537, 561, 705, 971
MalI GATC 2 cut(s) 237, 1013
MbiI CCGCTC 1 cut(s) 345
MboI GATC 2 cut(s) 235, 1011
MboII GAAGA 5 cut(s) 126, 310, 668, 916, 959
MluCI AATT 3 cut(s) 94, 795, 941
MlyI GAGTC 4 cut(s) 49, 59, 266, 391
MmeI TCCRAC 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 373
MseI TTAA 4 cut(s) 93, 492, 503, 1033
MslI CAYNNNNRTG 1 cut(s) 747
MspI CCGG 1 cut(s) 374
MspR9I CCNGG 2 cut(s) 465, 816
MvaI CCWGG 2 cut(s) 465, 816
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 4 cut(s) 67, 347, 743, 752
NaeI GCCGGC 1 cut(s) 375
NdeII GATC 2 cut(s) 235, 1011
NgoMIV GCCGGC 1 cut(s) 373
NlaIII CATG 3 cut(s) 596, 746, 966
NlaIV GGNNCC 2 cut(s) 349, 889
NmeAIII GCCGAG 1 cut(s) 27
NspI RCATGY 2 cut(s) 746, 966
PaeI GCATGC 1 cut(s) 746
PaeR7I CTCGAG 1 cut(s) 55
PaqCI CACCTGC 1 cut(s) 209
PdiI GCCGGC 1 cut(s) 375
PfeI GAWTC 2 cut(s) 365, 994
PkrI GCNGC 4 cut(s) 115, 379, 754, 917
PleI GAGTC 4 cut(s) 48, 59, 265, 391
PpsI GAGTC 4 cut(s) 48, 59, 265, 391
PshAI GACNNNNGTC 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 463, 814
PspGI CCWGG 2 cut(s) 463, 814
PspN4I GGNNCC 2 cut(s) 349, 889
PspPI GGNCC 4 cut(s) 60, 328, 467, 854
PspXI VCTCGAGB 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 785
RsaI GTAC 1 cut(s) 958
RsaNI GTAC 1 cut(s) 957
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 4 cut(s) 93, 492, 503, 1033
SatI GCNGC 4 cut(s) 114, 378, 753, 916
Sau3AI GATC 2 cut(s) 235, 1011
Sau96I GGNCC 4 cut(s) 60, 328, 467, 854
SchI GAGTC 4 cut(s) 49, 59, 266, 391
ScrFI CCNGG 2 cut(s) 465, 816
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 4 cut(s) 360, 523, 534, 724
Sfr274I CTCGAG 1 cut(s) 55
SinI GGWCC 2 cut(s) 328, 854
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 55, 71
SmoI CTYRAG 2 cut(s) 55, 71
SphI GCATGC 1 cut(s) 746
Sse9I AATT 3 cut(s) 94, 795, 941
SsiI CCGC 4 cut(s) 113, 141, 345, 456
SspMI CTAG 4 cut(s) 537, 561, 705, 971
StyD4I CCNGG 2 cut(s) 463, 814
TaaI ACNGT 3 cut(s) 124, 337, 764
TaqI TCGA 4 cut(s) 56, 127, 150, 368
TasI AATT 3 cut(s) 94, 795, 941
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 2 cut(s) 365, 994
Tru1I TTAA 4 cut(s) 93, 492, 503, 1033
Tru9I TTAA 4 cut(s) 93, 492, 503, 1033
TscAI CASTG 4 cut(s) 127, 342, 638, 767
TseI GCWGC 3 cut(s) 377, 752, 915
TspDTI ATGAA 2 cut(s) 234, 634
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 4 cut(s) 127, 342, 638, 767
VpaK11BI GGWCC 2 cut(s) 328, 854
XbaI TCTAGA 1 cut(s) 560
XceI RCATGY 2 cut(s) 746, 966
XhoI CTCGAG 1 cut(s) 55
XspI CTAG 4 cut(s) 537, 561, 705, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.