MD15G1366900.v1.1

methyltransferase At1g78140, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007867
Physical Location & Seq
Reverse (-)
44655348 .. 44658418
3071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1366900.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGCGACGGTGGTCGGCAACCTGTCCTCGCCGTTTCTTCTGAGTCAACTCGGTAACTCGAGGTCTTGCTTTCTCAAGCTCAGGCCTTGTGCTACTTTATCGGTTTTCAAGCGCTCTTTCGCGGCGGCCATCAAAGTTCGAGCTGCTGCTTCCGCCGCCGCCGTCGAAGTCGAGCCAACCGAACCAATTGTGGTACAAAATGATGGCCTCAGCAGCAACAACATTTTAGCTTGTCCGATATGCTACGATCCATTTTCAAGTTCCGGTGATCCCGGCTTATCGGTCGAGTCTGCTTCTGGGTCCAGTTTTCATTGTAGCACTTGTAAGAAGACCTACTTTGGCAACGAAACACATATTGACCTGACAACAGCAAGCGGCGCCAAGAGCTATGGTGAATCAATGCCCGTTATCACGGAAATGTTTAGGACTCCATTGATATCGTATCTCTATGAGAGGGGTTGGCGTCAAAGCTTTACTGGATTGATTGGTCTTCCTGGCCCAGAGAAAGAGTTTGAGTGGACTAAAAATTTCTTGAAGCCTGTCTTGGGTGGTGGAAATATCATTGATGCAAGTTGTGGGAGTGGGCTATTTTCTAGACGTTTTGCCAAGAGTGGGTTGTTTTCTCTTGTTGTTGCTTTGGACTACTCAGAGAACATGTTGAAGGAATGCTATGGATTCATTCAGCAGGAGGAGAACTTCCCCAAAGAGAACGTAATTCTGGTCAGAGCTGATATATCTAGGCTTCCTTTTGCTACAAGTTCTGTTGATGCTGTGCATGCTGGTGCCGCCCTACATTGTTGGCCTTCACCATCAGCAGCTGTTGCTGAAATTAGTCGAGTCCTGCGACCTGGTGGGGTGTTTGTTGCATCTACTTACATACTGGACGGGCGTTTTTCGCTCATCCCATTTCTAAGGAACATAACACGGGTAACAAAGCAAATATCGGGCAGCCAAGTCTTCACATCAGAAAGTGAATTCGAAGATCTCTGCCAAGCATGTGGGCTAGTTGGTTTTACAAAAGTGAGGTATGGGCTTTTTGTGATGATCTCTGCCACAAAACCCACTTAA

Protein Analysis

356

Amino Acids

38.31

Weight (kDa)

7.96

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 181 - 288 1.5e-09 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 182 - 335 6.6e-08 Methyltransferase domain
Methyltransf_31 PF13847 184 - 293 2e-14 Methyltransferase domain
Methyltransf_25 PF13649 187 - 285 2.5e-17 Methyltransferase domain
Methyltransf_11 PF08241 189 - 288 1.9e-21 Methyltransferase domain
Methyltransf_12 PF08242 189 - 287 1.4e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 377, 782
AccII CGCG 1 cut(s) 122
AciI CCGC 7 cut(s) 122, 125, 153, 156, 159, 375, 786
AclWI GGATC 2 cut(s) 242, 263
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 2 cut(s) 526, 974
AcyI GRCGYC 2 cut(s) 378, 463
AfaI GTAC 1 cut(s) 195
AfeI AGCGCT 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 545, 612
AflIII ACRYGT 1 cut(s) 654
AgsI TTSAA 4 cut(s) 109, 258, 535, 661
AjnI CCWGG 2 cut(s) 493, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AluBI AGCT 7 cut(s) 79, 143, 230, 387, 471, 728, 818
AluI AGCT 7 cut(s) 79, 143, 230, 387, 471, 728, 818
AlwI GGATC 2 cut(s) 242, 263
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 58
Aor51HI AGCGCT 1 cut(s) 113
AoxI GGCC 5 cut(s) 83, 126, 205, 496, 800
ApeKI GCWGC 5 cut(s) 143, 146, 213, 815, 948
ApoI RAATTY 2 cut(s) 526, 974
AspLEI GCGC 2 cut(s) 114, 380
AspS9I GGNCC 2 cut(s) 300, 497
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 3 cut(s) 278, 404, 798
AsuII TTCGAA 1 cut(s) 978
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 1 cut(s) 300
BanI GGYRCC 2 cut(s) 377, 782
BbsI GAAGAC 3 cut(s) 335, 482, 949
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 5 cut(s) 130, 133, 225, 827, 960
BccI CCATC 3 cut(s) 137, 197, 817
BceAI ACGGC 2 cut(s) 16, 146
BciT130I CCWGG 2 cut(s) 495, 849
BcnI CCSGG 1 cut(s) 273
BfaI CTAG 3 cut(s) 594, 738, 1004
BfoI RGCGCY 2 cut(s) 115, 381
BglII AGATCT 1 cut(s) 982
Bme1390I CCNGG 3 cut(s) 273, 495, 849
Bme18I GGWCC 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 300, 497
BmiI GGNNCC 3 cut(s) 301, 379, 784
BmrFI CCNGG 3 cut(s) 273, 495, 849
BmsI GCATC 3 cut(s) 556, 757, 875
BoxI GACNNNNGTC 1 cut(s) 11
BpiI GAAGAC 3 cut(s) 335, 482, 949
Bpu10I CCTNAGC 2 cut(s) 80, 209
Bpu14I TTCGAA 1 cut(s) 978
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 1 cut(s) 273
BsaHI GRCGYC 2 cut(s) 378, 463
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 2 cut(s) 545, 612
Bse1I ACTGG 3 cut(s) 303, 481, 885
BseBI CCWGG 2 cut(s) 495, 849
BseGI GGATG 1 cut(s) 900
BseLI CCNNNNNNNGG 2 cut(s) 545, 612
BseMII CTCAG 4 cut(s) 32, 94, 223, 660
BseNI ACTGG 3 cut(s) 303, 481, 885
BseRI GAGGAG 1 cut(s) 704
BseXI GCAGC 5 cut(s) 130, 133, 225, 827, 960
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 5 cut(s) 85, 128, 207, 498, 802
BshNI GGYRCC 2 cut(s) 377, 782
BsiEI CGRYCG 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 2 cut(s) 264, 273
BslI CCNNNNNNNGG 2 cut(s) 545, 612
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 5 cut(s) 85, 128, 207, 498, 802
BsoBI CYCGRG 1 cut(s) 58
Bsp119I TTCGAA 1 cut(s) 978
Bsp143I GATC 4 cut(s) 247, 268, 982, 1044
BspACI CCGC 7 cut(s) 122, 125, 153, 156, 159, 375, 786
BspANI GGCC 5 cut(s) 85, 128, 207, 498, 802
BspCNI CTCAG 4 cut(s) 33, 93, 222, 659
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 3 cut(s) 301, 379, 784
BspPI GGATC 2 cut(s) 242, 263
BspT104I TTCGAA 1 cut(s) 978
BspT107I GGYRCC 2 cut(s) 377, 782
BsrI ACTGG 3 cut(s) 303, 481, 885
BssMI GATC 4 cut(s) 247, 268, 982, 1044
BssNI GRCGYC 2 cut(s) 378, 463
Bst2UI CCWGG 2 cut(s) 495, 849
Bst4CI ACNGT 1 cut(s) 10
BstACI GRCGYC 2 cut(s) 378, 463
BstAPI GCANNNNNTGC 1 cut(s) 821
BstBI TTCGAA 1 cut(s) 978
BstC8I GCNNGC 2 cut(s) 373, 777
BstDEI CTNAG 5 cut(s) 41, 80, 209, 646, 911
BstF5I GGATG 1 cut(s) 900
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 2 cut(s) 115, 381
BstHHI GCGC 2 cut(s) 114, 380
BstKTI GATC 4 cut(s) 250, 271, 985, 1047
BstMBI GATC 4 cut(s) 247, 268, 982, 1044
BstMCI CGRYCG 1 cut(s) 285
BstMWI GCNNNNNNNGC 9 cut(s) 152, 155, 213, 377, 384, 776, 785, 821, 895
BstNI CCWGG 2 cut(s) 495, 849
BstNSI RCATGY 3 cut(s) 658, 779, 999
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 3 cut(s) 271, 493, 847
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 5 cut(s) 130, 133, 225, 827, 960
BstV2I GAAGAC 3 cut(s) 335, 482, 949
BstX2I RGATCY 1 cut(s) 982
BstXI CCANNNNNNTGG 1 cut(s) 998
BstYI RGATCY 1 cut(s) 982
BsuRI GGCC 5 cut(s) 85, 128, 207, 498, 802
BtsCI GGATG 1 cut(s) 900
Cac8I GCNNGC 2 cut(s) 373, 777
CaiI CAGNNNCTG 1 cut(s) 818
CfoI GCGC 2 cut(s) 114, 380
Cfr13I GGNCC 2 cut(s) 300, 497
CseI GACGC 1 cut(s) 452
CsiI ACCWGGT 1 cut(s) 847
Csp6I GTAC 1 cut(s) 194
CviAII CATG 3 cut(s) 655, 776, 996
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 5 cut(s) 41, 80, 209, 646, 911
DinI GGCGCC 1 cut(s) 379
DpnI GATC 4 cut(s) 249, 270, 984, 1046
DpnII GATC 4 cut(s) 247, 268, 982, 1044
EaeI YGGCCR 1 cut(s) 126
EciI GGCGGA 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 85
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 300
Eco47III AGCGCT 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 974
EcoRII CCWGG 2 cut(s) 493, 847
EcoRV GATATC 1 cut(s) 438
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
FaeI CATG 3 cut(s) 658, 779, 999
FalI AAGNNNNNCTT 4 cut(s) 320, 352, 527, 559
FatI CATG 3 cut(s) 654, 775, 995
FokI GGATG 1 cut(s) 887
FspBI CTAG 3 cut(s) 594, 738, 1004
GlaI GCGC 2 cut(s) 113, 379
HaeII RGCGCY 2 cut(s) 115, 381
HaeIII GGCC 5 cut(s) 85, 128, 207, 498, 802
HapII CCGG 2 cut(s) 264, 273
HgaI GACGC 1 cut(s) 452
HhaI GCGC 2 cut(s) 114, 380
Hin1I GRCGYC 2 cut(s) 378, 463
Hin1II CATG 3 cut(s) 658, 779, 999
Hin6I GCGC 2 cut(s) 112, 378
HinP1I GCGC 2 cut(s) 112, 378
HincII GTYRAC 1 cut(s) 47
HindII GTYRAC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 469
HinfI GANTC 6 cut(s) 43, 287, 395, 427, 675, 837
HpaII CCGG 2 cut(s) 264, 273
HphI GGTGA 3 cut(s) 278, 404, 798
Hpy166II GTNNAC 2 cut(s) 47, 519
Hpy188I TCNGA 5 cut(s) 42, 237, 649, 725, 967
Hpy188III TCNNGA 2 cut(s) 532, 594
Hpy8I GTNNAC 2 cut(s) 47, 519
Hpy99I CGWCG 2 cut(s) 10, 167
HpyAV CCTTC 2 cut(s) 655, 813
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 2 cut(s) 598, 711
HpyCH4V TGCA 3 cut(s) 569, 775, 866
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 155, 213, 377, 384, 776, 785, 821, 895
HpyF3I CTNAG 5 cut(s) 41, 80, 209, 646, 911
HpySE526I ACGT 2 cut(s) 598, 711
Hsp92I GRCGYC 2 cut(s) 378, 463
Hsp92II CATG 3 cut(s) 658, 779, 999
HspAI GCGC 2 cut(s) 112, 378
KasI GGCGCC 1 cut(s) 377
Kzo9I GATC 4 cut(s) 247, 268, 982, 1044
Lsp1109I GCAGC 5 cut(s) 130, 133, 225, 827, 960
LweI GCATC 3 cut(s) 556, 757, 875
MabI ACCWGGT 1 cut(s) 847
MaeI CTAG 3 cut(s) 594, 738, 1004
MaeII ACGT 2 cut(s) 598, 711
MaeIII GTNAC 2 cut(s) 53, 928
MalI GATC 4 cut(s) 249, 270, 984, 1046
MboI GATC 4 cut(s) 247, 268, 982, 1044
MboII GAAGA 5 cut(s) 29, 340, 482, 949, 992
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 1 cut(s) 982
MluCI AATT 5 cut(s) 186, 526, 714, 828, 974
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 4 cut(s) 52, 296, 421, 846
MnlI CCTC 6 cut(s) 37, 54, 218, 447, 682, 1017
MseI TTAA 1 cut(s) 1066
MslI CAYNNNNRTG 2 cut(s) 416, 780
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 2 cut(s) 264, 273
MspR9I CCNGG 3 cut(s) 273, 495, 849
MunI CAATTG 1 cut(s) 186
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 2 cut(s) 495, 849
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 9 cut(s) 152, 155, 213, 377, 384, 776, 785, 821, 895
NarI GGCGCC 1 cut(s) 378
NciI CCSGG 1 cut(s) 273
NdeII GATC 4 cut(s) 247, 268, 982, 1044
NlaIII CATG 3 cut(s) 658, 779, 999
NlaIV GGNNCC 3 cut(s) 301, 379, 784
NspI RCATGY 3 cut(s) 658, 779, 999
NspV TTCGAA 1 cut(s) 978
PaeI GCATGC 1 cut(s) 779
PaeR7I CTCGAG 1 cut(s) 58
PceI AGGCCT 1 cut(s) 85
PciI ACATGT 1 cut(s) 654
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 841
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 2 cut(s) 395, 675
PleI GAGTC 4 cut(s) 51, 295, 421, 845
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 4 cut(s) 51, 295, 421, 845
PscI ACATGT 1 cut(s) 654
PshAI GACNNNNGTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 493, 847
PspGI CCWGG 2 cut(s) 493, 847
PspN4I GGNNCC 3 cut(s) 301, 379, 784
PspPI GGNCC 2 cut(s) 300, 497
PspXI VCTCGAGB 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 982
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 416, 780
SaqAI TTAA 1 cut(s) 1066
Sau3AI GATC 4 cut(s) 247, 268, 982, 1044
Sau96I GGNCC 2 cut(s) 300, 497
SchI GAGTC 4 cut(s) 52, 296, 421, 846
ScrFI CCNGG 3 cut(s) 273, 495, 849
SexAI ACCWGGT 1 cut(s) 847
SfaNI GCATC 3 cut(s) 556, 757, 875
SfoI GGCGCC 1 cut(s) 379
Sfr274I CTCGAG 1 cut(s) 58
SfuI TTCGAA 1 cut(s) 978
SinI GGWCC 1 cut(s) 300
SlaI CTCGAG 1 cut(s) 58
SmiMI CAYNNNNRTG 2 cut(s) 416, 780
SmlI CTYRAG 2 cut(s) 58, 74
SmoI CTYRAG 2 cut(s) 58, 74
SphI GCATGC 1 cut(s) 779
Sse9I AATT 5 cut(s) 186, 526, 714, 828, 974
SseBI AGGCCT 1 cut(s) 85
SsiI CCGC 7 cut(s) 122, 125, 153, 156, 159, 375, 786
SspDI GGCGCC 1 cut(s) 377
SspMI CTAG 3 cut(s) 594, 738, 1004
StuI AGGCCT 1 cut(s) 85
StyD4I CCNGG 3 cut(s) 271, 493, 847
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 2 cut(s) 601, 714
TaqI TCGA 7 cut(s) 59, 139, 165, 171, 285, 835, 978
TaqII GACCGA 1 cut(s) 271
TasI AATT 5 cut(s) 186, 526, 714, 828, 974
TauI GCSGC 6 cut(s) 125, 128, 158, 161, 378, 788
TfiI GAWTC 2 cut(s) 395, 675
Tru1I TTAA 1 cut(s) 1066
Tru9I TTAA 1 cut(s) 1066
TseI GCWGC 5 cut(s) 143, 146, 213, 815, 948
TspDTI ATGAA 2 cut(s) 299, 667
TspGWI ACGGA 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 300
XapI RAATTY 2 cut(s) 526, 974
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 3 cut(s) 658, 779, 999
XhoI CTCGAG 1 cut(s) 58
XspI CTAG 3 cut(s) 594, 738, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.