MD08G1181500.v1.1

methyltransferase At1g78140, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007867
Physical Location & Seq
Reverse (-)
22500908 .. 22504081
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1181500.v1.1.491

Sequence Viewer

Length: 822 bp
ATGGCTTCTCCTTTTTATTGTATGGCATGCTTTTGCAGTGAGTCTGTTTCTGGGTCTAGTTTTCAATGTAGGACTTGTAAGAAGACTTACTTTGGCAACGAAACACATATTGACCTGACAACAGCAAGTGGCGCAAAGGACTATGGGGAATCAAAGCCCGTTTCCACAGAAATGTTTAGGACTCCATTGGTATCGTACCTCTATGAGAGGGGTTGGCGTCAAAGCTTTACTGTATGGGGTGGATTTCCTGGCCCAGAGAAAGAGTTTGAGTTGATTAAGGATTTCTTAAAGCCAGTCTTGGGTGGTGGAAATATCATTGATGCAAGTTGTGGGAGTGGGATGTTTTCTAGACTTTTTGCTAAGAGCGGATTATTTTCTCTTGTTGTTGCCTTGGACTACTCAGAGAACATGTTGAAAGAGTGCTATGGATTCATTCAGCAGGAGGATAACTTTCCCAAAGAGAACATGATCCTGGTCAGAGCTGATATTTCTAGGCTTCCCTTTGCTACTAGTTCTGTCGATGCAGTGCATGCTGGTGCTGCCATACACTGTTGGCCTTCACCATCATCAGCTGTTGCTGAAATTAGTCGAGTCCTGCGACCCGGCGGGGTGTTTGTTGCAACCACTTTCATATGGGACGGGCCTTTTTCGCTCATCCCATTTCAAAGGAACATAGCACAGAGAACAAAGCAAATGTCGGGCAACCAACTCTTCACGTCAGAAAGTGAATTACAAGATATCTGCCAAGCATGTGGACTGGTAGGTTTTACCAAAGTGAGGAATGGGCGTTTTGTGATGATTTCTGCCACAAAACCCATTTAA

Protein Analysis

274

Amino Acids

30.1

Weight (kDa)

7.48

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 91 - 253 4.6e-09 Methyltransferase domain
Ubie_methyltran PF01209 99 - 207 3e-09 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 102 - 211 9.6e-14 Methyltransferase domain
Methyltransf_25 PF13649 105 - 203 2.2e-16 Methyltransferase domain
Methyltransf_11 PF08241 107 - 206 5.1e-21 Methyltransferase domain
Methyltransf_12 PF08242 107 - 205 4.8e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 366
AciI CCGC 2 cut(s) 366, 606
AclWI GGATC 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 217
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 299, 777
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 3 cut(s) 65, 415, 665
AhlI ACTAGT 1 cut(s) 509
AjiI CACGTC 1 cut(s) 717
AjnI CCWGG 2 cut(s) 247, 471
AluBI AGCT 3 cut(s) 225, 482, 572
AluI AGCT 3 cut(s) 225, 482, 572
AlwI GGATC 1 cut(s) 463
AoxI GGCC 3 cut(s) 250, 554, 641
ApeKI GCWGC 1 cut(s) 539
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 2 cut(s) 251, 641
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 526
BccI CCATC 1 cut(s) 571
BciT130I CCWGG 2 cut(s) 249, 473
BcnI CCSGG 1 cut(s) 603
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 4 cut(s) 57, 348, 492, 510
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 3 cut(s) 249, 473, 603
BmgBI CACGTC 1 cut(s) 717
BmgT120I GGNCC 2 cut(s) 251, 641
BmrFI CCNGG 3 cut(s) 249, 473, 603
BmsI GCATC 2 cut(s) 310, 511
BpiI GAAGAC 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 390
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 777
Bse1I ACTGG 2 cut(s) 293, 762
BseBI CCWGG 2 cut(s) 249, 473
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 2 cut(s) 345, 654
BseLI CCNNNNNNNGG 2 cut(s) 299, 777
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 293, 762
BseXI GCAGC 1 cut(s) 526
BshFI GGCC 3 cut(s) 252, 556, 643
BsiSI CCGG 1 cut(s) 603
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 2 cut(s) 299, 777
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 3 cut(s) 252, 556, 643
Bsp143I GATC 1 cut(s) 468
BspACI CCGC 2 cut(s) 366, 606
BspANI GGCC 3 cut(s) 252, 556, 643
BspCNI CTCAG 1 cut(s) 413
BspPI GGATC 1 cut(s) 463
BsrBI CCGCTC 1 cut(s) 366
BsrI ACTGG 2 cut(s) 293, 762
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 1 cut(s) 468
BssNI GRCGYC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 249, 473
Bst4CI ACNGT 2 cut(s) 232, 551
Bst6I CTCTTC 1 cut(s) 716
BstACI GRCGYC 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 530
BstC8I GCNNGC 2 cut(s) 28, 531
BstDEI CTNAG 2 cut(s) 360, 400
BstF5I GGATG 2 cut(s) 345, 654
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 1 cut(s) 471
BstMBI GATC 1 cut(s) 468
BstMWI GCNNNNNNNGC 4 cut(s) 131, 530, 539, 649
BstNI CCWGG 2 cut(s) 249, 473
BstNSI RCATGY 4 cut(s) 30, 412, 533, 753
BstSCI CCNGG 3 cut(s) 247, 471, 601
BstV1I GCAGC 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 89
BstXI CCANNNNNNTGG 1 cut(s) 752
BsuRI GGCC 3 cut(s) 252, 556, 643
BtrI CACGTC 1 cut(s) 717
BtsCI GGATG 2 cut(s) 345, 654
BtsI GCAGTG 2 cut(s) 43, 531
BtsIMutI CAGTG 3 cut(s) 43, 531, 547
Cac8I GCNNGC 2 cut(s) 28, 531
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 251, 641
CseI GACGC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 196
CviAII CATG 5 cut(s) 27, 409, 466, 530, 750
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 360, 400
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
Eco130I CCWWGG 1 cut(s) 390
Eco32I GATATC 1 cut(s) 739
EcoRII CCWGG 2 cut(s) 247, 471
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 30, 412, 469, 533, 753
FalI AAGNNNNNCTT 6 cut(s) 74, 106, 269, 301, 281, 313
FaqI GGGAC 1 cut(s) 650
FatI CATG 5 cut(s) 26, 408, 465, 529, 749
FauI CCCGC 1 cut(s) 599
FauNDI CATATG 1 cut(s) 632
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 2 cut(s) 352, 641
Fsp4HI GCNGC 1 cut(s) 540
FspBI CTAG 4 cut(s) 57, 348, 492, 510
GlaI GCGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 540
HaeIII GGCC 3 cut(s) 252, 556, 643
HapII CCGG 1 cut(s) 603
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 5 cut(s) 30, 412, 469, 533, 753
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 223
HinfI GANTC 5 cut(s) 41, 149, 181, 429, 591
HpaII CCGG 1 cut(s) 603
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 1 cut(s) 755
Hpy188I TCNGA 3 cut(s) 403, 479, 721
Hpy188III TCNNGA 1 cut(s) 348
Hpy8I GTNNAC 1 cut(s) 755
HpyAV CCTTC 1 cut(s) 567
HpyCH4III ACNGT 2 cut(s) 232, 551
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 5 cut(s) 36, 323, 524, 529, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 530, 539, 649
HpyF3I CTNAG 2 cut(s) 360, 400
HpySE526I ACGT 1 cut(s) 716
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 5 cut(s) 30, 412, 469, 533, 753
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 1 cut(s) 468
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 2 cut(s) 310, 511
MaeI CTAG 4 cut(s) 57, 348, 492, 510
MaeII ACGT 1 cut(s) 716
MalI GATC 1 cut(s) 470
MbiI CCGCTC 1 cut(s) 366
MboI GATC 1 cut(s) 468
MboII GAAGA 2 cut(s) 94, 703
MluCI AATT 2 cut(s) 582, 728
MlyI GAGTC 3 cut(s) 50, 175, 600
MnlI CCTC 4 cut(s) 201, 209, 436, 771
MseI TTAA 3 cut(s) 276, 287, 820
MslI CAYNNNNRTG 2 cut(s) 170, 534
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 3 cut(s) 249, 473, 603
MvaI CCWGG 2 cut(s) 249, 473
MwoI GCNNNNNNNGC 4 cut(s) 131, 530, 539, 649
NciI CCSGG 1 cut(s) 603
NdeI CATATG 1 cut(s) 632
NdeII GATC 1 cut(s) 468
NlaIII CATG 5 cut(s) 30, 412, 469, 533, 753
NspI RCATGY 4 cut(s) 30, 412, 533, 753
PaeI GCATGC 2 cut(s) 30, 533
PciI ACATGT 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PfeI GAWTC 2 cut(s) 149, 429
PkrI GCNGC 1 cut(s) 541
PleI GAGTC 3 cut(s) 49, 175, 599
PpsI GAGTC 3 cut(s) 49, 175, 599
PscI ACATGT 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 247, 471
PspGI CCWGG 2 cut(s) 247, 471
PspPI GGNCC 2 cut(s) 251, 641
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 2 cut(s) 170, 534
SaqAI TTAA 3 cut(s) 276, 287, 820
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 1 cut(s) 468
Sau96I GGNCC 2 cut(s) 251, 641
SchI GAGTC 3 cut(s) 50, 175, 600
ScrFI CCNGG 3 cut(s) 249, 473, 603
SetI ASST 7 cut(s) 117, 201, 227, 484, 574, 719, 766
SfaNI GCATC 2 cut(s) 310, 511
SmiMI CAYNNNNRTG 2 cut(s) 170, 534
SpeI ACTAGT 1 cut(s) 509
SphI GCATGC 2 cut(s) 30, 533
Sse9I AATT 2 cut(s) 582, 728
SsiI CCGC 2 cut(s) 366, 606
SspMI CTAG 4 cut(s) 57, 348, 492, 510
StyD4I CCNGG 3 cut(s) 247, 471, 601
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 232, 551
TaiI ACGT 1 cut(s) 719
TaqI TCGA 2 cut(s) 519, 589
TasI AATT 2 cut(s) 582, 728
TfiI GAWTC 2 cut(s) 149, 429
Tru1I TTAA 3 cut(s) 276, 287, 820
Tru9I TTAA 3 cut(s) 276, 287, 820
TscAI CASTG 3 cut(s) 43, 531, 554
TseI GCWGC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 421, 619
TspRI CASTG 3 cut(s) 43, 531, 554
XbaI TCTAGA 1 cut(s) 347
XceI RCATGY 4 cut(s) 30, 412, 533, 753
XspI CTAG 4 cut(s) 57, 348, 492, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.