RLG00000000762

methyltransferase At1g78140, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007867
Physical Location & Seq
Reverse (-)
5071458 .. 5075744
4287 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000762

Sequence Viewer

Length: 1002 bp
ATGGCGGCGACGACCCTCGTCAGCAACCTCTCTCTCCCGAGTCAACTCGGCAACTCGAGGCCCGACTCGCTCAAGCCCAACCCCTTCGCCGTTAATTTCAAACGCTCTTTCGCGGCCATCACTGTTCGAGCTTCTTCCACCGCTTTCGTCGAGACCACTCCAACTGACCCAGTTGAGGTTCAGGATAATGAGGCCACCACCTGCAACGACTTGTTTGCTTGTCCTATATGCTTTGATCCATTTTCATCAAATGGGGAGTCTGGTTCAGGGTCAGGTTTTGAATGTAGCACTTGTAAGAAGACATACTTTTGCAATGAGATACATTTGGACCTGACAGTGGCGAGCGGTGCGAAGAACTACGGTGAATCGATGCCAGTTTCTACCGAGATGTTCAGGACTCCGTTGGTATCTTTCCTCTATGAGAGGGGCTGGCGTCAAAGCTTTTCGGTATGGGGCGGCTTTCCTGGCCCAGAGAAAGAGTTTGAGTTGATTAAGGATTACTTAAAACCAGTCTTGGGTGGAAGCATCATTGATGCTAGTTGTGGGAGTGGATTATTTTCTAGACTTTTCGCCAAGAGTGGATTGTTTTCTCATGTTGTTGCTCTTGACTACTCAGAAAATATGTTGAAGCAGTGCTATGAGTTCATTGAAAAGGAAGAGAACTTCCCCAAAGAGAACATAACTTTGGTCAGAGCTGATATTTCTAGGCTTCCCTTTGCTACAAGTTCTGTTGATGCTGTGCATGCTGGTGCTGCGATACATTGTTGGCCTTCACCATCAGGAGCTTGTTCCTTGTGGTTTGGGATTATATTTACAGTTGACACTGTTTTTTCTTTTATTCTTTTCCCTCACAATATCATTAGAATAAGGCAAATATCGGGCAGCCAAATCTTCTTATCTGAAGGTGAATTAGAAGACATCTGTACAGCATGTGGGCTTGTGGGTTTTACAGCCGTGAGGAATCGGTTATTTGTGATGATCTCTGCCACAAAACCAAGTTAA

Protein Analysis

334

Amino Acids

36.38

Weight (kDa)

5.23

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 174 - 261 6e-10 Methyltransferase domain
Methyltransf_25 PF13649 176 - 258 2e-13 Methyltransferase domain
Methyltransf_11 PF08241 178 - 263 2.4e-14 Methyltransferase domain
Methyltransf_12 PF08242 178 - 260 2.7e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 1 cut(s) 113
AciI CCGC 5 cut(s) 5, 113, 141, 345, 456
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 114
AcuI CTGAAG 1 cut(s) 921
AcyI GRCGYC 1 cut(s) 433
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 3 cut(s) 175, 337, 515
AgsI TTSAA 4 cut(s) 100, 281, 628, 650
AjnI CCWGG 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 4 cut(s) 131, 441, 695, 785
AluI AGCT 4 cut(s) 131, 441, 695, 785
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 1 cut(s) 230
Ama87I CYCGRG 2 cut(s) 37, 55
AoxI GGCC 5 cut(s) 59, 114, 192, 466, 767
ApeKI GCWGC 2 cut(s) 752, 882
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspS9I GGNCC 3 cut(s) 60, 328, 467
AsuHPI GGTGA 3 cut(s) 374, 765, 917
AvaI CYCGRG 2 cut(s) 37, 55
AvaII GGWCC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 305, 921
BbvI GCAGC 2 cut(s) 739, 894
BccI CCATC 2 cut(s) 125, 784
BceAI ACGGC 2 cut(s) 74, 938
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 3 cut(s) 537, 561, 705
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 5 cut(s) 6, 114, 457, 753, 883
BlsI GCNGC 5 cut(s) 7, 115, 458, 754, 884
Bme1390I CCNGG 1 cut(s) 465
Bme18I GGWCC 1 cut(s) 328
BmeT110I CYCGRG 2 cut(s) 37, 55
BmgT120I GGNCC 3 cut(s) 60, 328, 467
BmrFI CCNGG 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 4 cut(s) 360, 523, 534, 724
BmuI ACTGGG 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 305, 921
BpuEI CTTGAG 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 146
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 337, 515
Bse1I ACTGG 3 cut(s) 170, 374, 509
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 368
BseLI CCNNNNNNNGG 3 cut(s) 175, 337, 515
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 3 cut(s) 170, 374, 509
BseXI GCAGC 2 cut(s) 739, 894
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 5 cut(s) 61, 116, 194, 468, 769
BshVI ATCGAT 1 cut(s) 368
BsiHKCI CYCGRG 2 cut(s) 37, 55
BslI CCNNNNNNNGG 3 cut(s) 175, 337, 515
BsmAI GTCTC 1 cut(s) 146
BsnI GGCC 5 cut(s) 61, 116, 194, 468, 769
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 2 cut(s) 37, 55
Bsp1407I TGTACA 1 cut(s) 923
Bsp143I GATC 2 cut(s) 235, 978
BspACI CCGC 5 cut(s) 5, 113, 141, 345, 456
BspANI GGCC 5 cut(s) 61, 116, 194, 468, 769
BspCNI CTCAG 1 cut(s) 626
BspDI ATCGAT 1 cut(s) 368
BspFNI CGCG 1 cut(s) 113
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 923
BsrI ACTGG 3 cut(s) 170, 374, 509
BssMI GATC 2 cut(s) 235, 978
BssNI GRCGYC 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 5 cut(s) 124, 337, 362, 817, 826
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 1 cut(s) 433
BstAUI TGTACA 1 cut(s) 923
BstC8I GCNNGC 3 cut(s) 343, 431, 744
BstDEI CTNAG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 2 cut(s) 238, 981
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 2 cut(s) 235, 978
BstMWI GCNNNNNNNGC 5 cut(s) 67, 347, 465, 743, 752
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 2 cut(s) 746, 933
BstPAI GACNNNNGTC 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 463
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 739, 894
BstV2I GAAGAC 2 cut(s) 305, 921
Bsu15I ATCGAT 1 cut(s) 368
BsuRI GGCC 5 cut(s) 61, 116, 194, 468, 769
BsuTUI ATCGAT 1 cut(s) 368
BtsI GCAGTG 1 cut(s) 638
BtsIMutI CAGTG 4 cut(s) 120, 342, 638, 822
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 3 cut(s) 343, 431, 744
Cfr13I GGNCC 3 cut(s) 60, 328, 467
ClaI ATCGAT 1 cut(s) 368
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 924
CviAII CATG 3 cut(s) 593, 743, 930
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 1 cut(s) 613
DpnI GATC 2 cut(s) 237, 980
DpnII GATC 2 cut(s) 235, 978
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 921
Eco88I CYCGRG 2 cut(s) 37, 55
EcoRII CCWGG 1 cut(s) 463
FaeI CATG 3 cut(s) 596, 746, 933
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 485, 517
FatI CATG 3 cut(s) 592, 742, 929
Fnu4HI GCNGC 5 cut(s) 6, 114, 457, 753, 883
Fsp4HI GCNGC 5 cut(s) 6, 114, 457, 753, 883
FspBI CTAG 3 cut(s) 537, 561, 705
GluI GCNGC 5 cut(s) 6, 114, 457, 753, 883
HaeIII GGCC 5 cut(s) 61, 116, 194, 468, 769
HgaI GACGC 1 cut(s) 422
Hin1I GRCGYC 1 cut(s) 433
Hin1II CATG 3 cut(s) 596, 746, 933
HincII GTYRAC 2 cut(s) 44, 820
HindII GTYRAC 2 cut(s) 44, 820
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 6 cut(s) 40, 65, 257, 365, 397, 961
HphI GGTGA 3 cut(s) 374, 765, 917
Hpy166II GTNNAC 2 cut(s) 44, 820
Hpy188I TCNGA 3 cut(s) 616, 692, 901
Hpy188III TCNNGA 7 cut(s) 37, 151, 182, 394, 561, 605, 780
Hpy8I GTNNAC 2 cut(s) 44, 820
Hpy99I CGWCG 2 cut(s) 13, 152
HpyAV CCTTC 3 cut(s) 94, 780, 896
HpyCH4III ACNGT 5 cut(s) 124, 337, 362, 817, 826
HpyCH4V TGCA 3 cut(s) 204, 312, 742
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 347, 465, 743, 752
HpyF3I CTNAG 1 cut(s) 613
Hsp92I GRCGYC 1 cut(s) 433
Hsp92II CATG 3 cut(s) 596, 746, 933
Kzo9I GATC 2 cut(s) 235, 978
LmnI GCTCC 1 cut(s) 782
Lsp1109I GCAGC 2 cut(s) 739, 894
LweI GCATC 4 cut(s) 360, 523, 534, 724
MaeI CTAG 3 cut(s) 537, 561, 705
MalI GATC 2 cut(s) 237, 980
MbiI CCGCTC 1 cut(s) 345
MboI GATC 2 cut(s) 235, 978
MboII GAAGA 6 cut(s) 126, 310, 364, 668, 883, 926
MluCI AATT 2 cut(s) 94, 908
MlyI GAGTC 4 cut(s) 49, 59, 266, 391
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 9 cut(s) 26, 38, 51, 169, 184, 417, 425, 858, 951
MseI TTAA 4 cut(s) 93, 492, 503, 1000
MslI CAYNNNNRTG 1 cut(s) 747
MspR9I CCNGG 1 cut(s) 465
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 5 cut(s) 67, 347, 465, 743, 752
NdeII GATC 2 cut(s) 235, 978
NlaIII CATG 3 cut(s) 596, 746, 933
NmeAIII GCCGAG 1 cut(s) 27
NspI RCATGY 2 cut(s) 746, 933
PaeI GCATGC 1 cut(s) 746
PaeR7I CTCGAG 1 cut(s) 55
PaqCI CACCTGC 1 cut(s) 209
PfeI GAWTC 2 cut(s) 365, 961
PkrI GCNGC 5 cut(s) 7, 115, 458, 754, 884
PleI GAGTC 4 cut(s) 48, 59, 265, 391
PpsI GAGTC 4 cut(s) 48, 59, 265, 391
PshAI GACNNNNGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspPI GGNCC 3 cut(s) 60, 328, 467
PspXI VCTCGAGB 1 cut(s) 55
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 4 cut(s) 93, 492, 503, 1000
SatI GCNGC 5 cut(s) 6, 114, 457, 753, 883
Sau3AI GATC 2 cut(s) 235, 978
Sau96I GGNCC 3 cut(s) 60, 328, 467
SchI GAGTC 4 cut(s) 49, 59, 266, 391
ScrFI CCNGG 1 cut(s) 465
SfaNI GCATC 4 cut(s) 360, 523, 534, 724
Sfr274I CTCGAG 1 cut(s) 55
SinI GGWCC 1 cut(s) 328
SlaI CTCGAG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 55, 71
SmoI CTYRAG 2 cut(s) 55, 71
SphI GCATGC 1 cut(s) 746
Sse9I AATT 2 cut(s) 94, 908
SsiI CCGC 5 cut(s) 5, 113, 141, 345, 456
SspMI CTAG 3 cut(s) 537, 561, 705
StyD4I CCNGG 1 cut(s) 463
TaaI ACNGT 5 cut(s) 124, 337, 362, 817, 826
TaqI TCGA 4 cut(s) 56, 127, 150, 368
TasI AATT 2 cut(s) 94, 908
TatI WGTACW 1 cut(s) 923
TauI GCSGC 3 cut(s) 8, 116, 459
TfiI GAWTC 2 cut(s) 365, 961
Tru1I TTAA 4 cut(s) 93, 492, 503, 1000
Tru9I TTAA 4 cut(s) 93, 492, 503, 1000
TscAI CASTG 4 cut(s) 127, 342, 638, 829
TseI GCWGC 2 cut(s) 752, 882
TspDTI ATGAA 2 cut(s) 234, 634
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 4 cut(s) 127, 342, 638, 829
VpaK11BI GGWCC 1 cut(s) 328
XbaI TCTAGA 1 cut(s) 560
XceI RCATGY 2 cut(s) 746, 933
XhoI CTCGAG 1 cut(s) 55
XspI CTAG 3 cut(s) 537, 561, 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.