FvH4_6g05691

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013896
Physical Location & Seq
Reverse (-)
3244879 .. 3250022
5144 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05691.t1

Sequence Viewer

Length: 1800 bp
ATGCGGATGTCCGCAGCTAAGAAGGACTGTATATATATATACGGAAACGATGTGGAGCGCACGTCCGTAGTGCGCACGAAGCTCCGTTCGCCACCCTCCGGCGCCGACGACGACGCACTTTCACCCCAGCAGACTCCAGCAGCGTCCCAGATCACTTTCCCTCCCAGGCGATCCCCGACGAGGCCGTCGAATTCCAGTTTCCGGCGAGATCGACTTTATTTGAAGTTTTGGGTGATCTTGCCAGAAAACTGGAATTCGGCGGACTCGTCGGGGAGGGAAAATGATGGGGGACACTGCTGGAGTGGGGTGGAGGCGCACCGTCATCGGCGTCGGACGGATGCAGATATAATGTGGCCTGGTCTTGCTGAACCAACGAATCCTCCGTGTGATCTGTGCTGCGATATCTGCAGTGAGCCTGGTTATGATGGATGGCTGTATAGGTGTCACATATGTGAGTTTCATGCTCATCTTTCTTGTGGCATTTCTGATAAAAAACTTCTTGATAGTTCTCAAGGTTACAATATTGAAGGCAAGGAGCTCTTGCAGTTGTCCTATTCTAATTCACCCTATGGTGAGAAAAACGAAGGGAGGAAAGAAAATGGTCATCAAGGTCCAAAGGGTGGTGCTGAATTAGTCCAAGTTACGTTTGAGTCCATCAAAAGTACTATTACAAGCAACAAGGTCTTGAGCAATAAAGTAGGACCGGAAAATAGAATGGATGAAGCATTCTTGACTCGAAATGACACTCTTACTAGGAAGGAATTAGGCCTAGAGAAGAAGAGAAAATCAAGTGAACCCGTGGGGAGGTCAAACATTGGAAATCGATCAGAGTACCAAATCGGATACTCAAGAGTCAAGCGGTTCATTTTTCCAGATGCTCTGTGGGTGCTGCAATCCGGGATACTAAAGAAGTATCCGACAAAGCTAGCTTTCAAGTTCAGTTTAATGAGTCGACAGTTGCTGAAAAAAGAGGATGGCGGCGCCTCCGCTATAGGAGGCGGTGGTGTAAGTGAGGTGATAGATCCGTCAGTGCTTTCTGCCATGTTGGTGGTTTGGAGGGTGAAGGCGAAGGTCAAGATCCATGCGGAGGACAGCGGGATTTTCGTTTTCCCATTCAAGGAGACGGAAGAGCAGAATTGCATCCTACATGGGGGACCTTGGTTTGGGTCATTGTCAAGGGTTTCTGACTTGCCATGCGCAAGGAGAAGGTCCTTGCTCGACTTAGGAATATTTTGGGCTCGTTCATTCGGGCTGATTCGGCGGCGTTGCGGCGTAGGGAGGTTTCCTAGAAGACCCGAATCATGCACAATGTGCGGCGGCTGTTTTTGTAGCGGTGAACCCTCGCTTAATTTCTCATCGGAAGTGACAGTGGATCTGGACATGAAAATGGAGAAGGTGAGAGGGTTGTGTTGGGGTTGTGGGTTCTTTTCCCATGACCCCACGGGTTACGACGGTCTTCTGGAGTCAGCGGCGACGTCGCAGGTGGTACCGGAGGCTGTTAAAGCCAAGGAGGCCGGTCTAGAAACAATGGAAGAAGCCAAGGGTCATATTGCTATGGCGAAGGAATTTGAGAAGAAAGCAAAAAACAAGCTCGACGGGCGGAGAATTTTCAGTTCCAAAGAATCCAAACGCAAACAGGCCGCAAAGCTCTTCCATGAAGCTGCTCAACATTATGGACTCGCCAAAGCCTGGGATAAAGCTGGGGAAACATTGGTAAAGTCGACAAACTGCTATTCGGAGATTAAGGCCCAGTTTGGCCCCGCTGGTACCCTTAGATTATCAGTTCCATTGTCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

600

Amino Acids

66.57

Weight (kDa)

9.09

Isoelectric Point (pI)

57.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNAP PF14938 520 - 583 1.6e-09 Soluble NSF attachment protein, SNAP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42060
fragaria_vesca FvH4_6g05691 FvH4_6g22090
malus_domestica MD12G1056300.v1.1 MD14G1055700.v1.1
prunus_persica Prupe.7G078900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0479421
rosa_laevigata RLG00000023576
rosa_multiflora Rmu_sc0000107.1_g000006
rosa_roxburghii Rroxscaffold_6G00402290
rosa_rugosa Rorug03G0175200
rosa_samantha Rh3AG226500 Rh3BG259400 Rh3CG255300 Rh3DG250800 Rh3DG250900
rosa_wichuraiana Rw3G020200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1471
AasI GACNNNNNNGTC 1 cut(s) 184
AatII GACGTC 1 cut(s) 1478
Acc16I TGCGCA 2 cut(s) 74, 1198
Acc36I ACCTGC 1 cut(s) 1471
Acc65I GGTACC 2 cut(s) 1486, 1766
AccB1I GGYRCC 4 cut(s) 101, 980, 1486, 1766
AccB7I CCANNNNNTGG 2 cut(s) 620, 1689
AccI GTMKAC 2 cut(s) 952, 1721
AclWI GGATC 4 cut(s) 165, 1016, 1072, 1380
AcsI RAATTY 4 cut(s) 190, 253, 1565, 1605
AcyI GRCGYC 4 cut(s) 102, 328, 981, 1475
AdeI CACNNNGTG 1 cut(s) 1311
AfaI GTAC 4 cut(s) 664, 833, 1488, 1768
AgsI TTSAA 4 cut(s) 223, 527, 934, 1117
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 4 cut(s) 164, 355, 415, 1688
AleI CACNNNNGTG 1 cut(s) 450
AluBI AGCT 9 cut(s) 17, 82, 538, 925, 929, 1591, 1648, 1661, 1700
AluI AGCT 9 cut(s) 17, 82, 538, 925, 929, 1591, 1648, 1661, 1700
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 1 cut(s) 1115
AlwI GGATC 4 cut(s) 165, 1016, 1072, 1380
AlwNI CAGNNNCTG 1 cut(s) 961
AoxI GGCC 7 cut(s) 182, 353, 766, 1512, 1638, 1746, 1756
ApeKI GCWGC 5 cut(s) 14, 140, 396, 889, 1661
ApoI RAATTY 4 cut(s) 190, 253, 1565, 1605
Asp718I GGTACC 2 cut(s) 1486, 1766
AspLEI GCGC 6 cut(s) 60, 75, 104, 316, 983, 1199
AspS9I GGNCC 6 cut(s) 611, 701, 1154, 1209, 1747, 1757
AsuC2I CCSGG 1 cut(s) 898
AsuHPI GGTGA 8 cut(s) 114, 244, 555, 584, 1027, 1072, 1346, 1408
AsuNHI GCTAGC 1 cut(s) 925
AvaII GGWCC 4 cut(s) 611, 701, 1154, 1209
BanI GGYRCC 4 cut(s) 101, 980, 1486, 1766
BanII GRGCYC 2 cut(s) 540, 1240
BbsI GAAGAC 2 cut(s) 1297, 1448
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 5 cut(s) 26, 152, 383, 876, 1648
BccI CCATC 5 cut(s) 278, 419, 423, 662, 968
BceAI ACGGC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 4 cut(s) 166, 357, 417, 1690
BciVI GTATCC 3 cut(s) 836, 894, 924
BcnI CCSGG 1 cut(s) 898
BcoDI GTCTC 1 cut(s) 1115
BfaI CTAG 5 cut(s) 753, 770, 926, 1287, 1520
BfmI CTRYAG 2 cut(s) 406, 990
BfoI RGCGCY 2 cut(s) 105, 984
BfuAI ACCTGC 1 cut(s) 1471
BfuI GTATCC 3 cut(s) 836, 894, 924
BglI GCCNNNNNGGC 1 cut(s) 1511
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 5 cut(s) 166, 357, 417, 898, 1690
Bme18I GGWCC 4 cut(s) 611, 701, 1154, 1209
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 6 cut(s) 611, 701, 1154, 1209, 1747, 1757
BmiI GGNNCC 6 cut(s) 103, 982, 1155, 1488, 1759, 1768
BmrFI CCNGG 5 cut(s) 166, 357, 417, 898, 1690
BmrI ACTGGG 1 cut(s) 1744
BmsI GCATC 3 cut(s) 328, 865, 1149
BmtI GCTAGC 1 cut(s) 929
BmuI ACTGGG 1 cut(s) 1744
BpiI GAAGAC 2 cut(s) 1297, 1448
BpmI CTGGAG 3 cut(s) 120, 319, 1481
BpuEI CTTGAG 3 cut(s) 495, 706, 832
BpuMI CCSGG 1 cut(s) 898
Bsa29I ATCGAT 1 cut(s) 823
BsaHI GRCGYC 4 cut(s) 102, 328, 981, 1475
BsaJI CCNNGG 7 cut(s) 164, 798, 1157, 1440, 1506, 1539, 1689
BsaWI WCCGGW 2 cut(s) 703, 1489
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 145, 175, 364, 394
Bse118I RCCGGY 1 cut(s) 1514
Bse1I ACTGG 3 cut(s) 195, 254, 1750
BseBI CCWGG 4 cut(s) 166, 357, 417, 1690
BseCI ATCGAT 1 cut(s) 823
BseDI CCNNGG 7 cut(s) 164, 798, 1157, 1440, 1506, 1539, 1689
BseGI GGATG 6 cut(s) 12, 343, 434, 724, 979, 1140
BseNI ACTGG 3 cut(s) 195, 254, 1750
BseXI GCAGC 5 cut(s) 26, 152, 383, 876, 1648
BseYI CCCAGC 2 cut(s) 126, 1700
BshFI GGCC 7 cut(s) 184, 355, 768, 1514, 1640, 1748, 1758
BshNI GGYRCC 4 cut(s) 101, 980, 1486, 1766
BshVI ATCGAT 1 cut(s) 823
BsiHKAI GWGCWC 1 cut(s) 540
BsiSI CCGG 6 cut(s) 99, 202, 704, 897, 1490, 1515
BslFI GGGAC 3 cut(s) 130, 303, 1167
BsmAI GTCTC 1 cut(s) 1115
BsmBI CGTCTC 1 cut(s) 1115
BsmFI GGGAC 3 cut(s) 130, 303, 1167
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 7 cut(s) 184, 355, 768, 1514, 1640, 1748, 1758
Bsp1286I GDGCHC 2 cut(s) 540, 1240
Bsp143I GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
BspANI GGCC 7 cut(s) 184, 355, 768, 1514, 1640, 1748, 1758
BspDI ATCGAT 1 cut(s) 823
BspLI GGNNCC 6 cut(s) 103, 982, 1155, 1488, 1759, 1768
BspMAI CTGCAG 1 cut(s) 410
BspMI ACCTGC 1 cut(s) 1471
BspOI GCTAGC 1 cut(s) 929
BspPI GGATC 4 cut(s) 165, 1016, 1072, 1380
BspQI GCTCTTC 2 cut(s) 1122, 1655
BspT107I GGYRCC 4 cut(s) 101, 980, 1486, 1766
BsrFI RCCGGY 1 cut(s) 1514
BsrI ACTGG 3 cut(s) 195, 254, 1750
BssAI RCCGGY 1 cut(s) 1514
BssECI CCNNGG 7 cut(s) 164, 798, 1157, 1440, 1506, 1539, 1689
BssMI GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
BssNI GRCGYC 4 cut(s) 102, 328, 981, 1475
BssT1I CCWWGG 3 cut(s) 1157, 1506, 1539
Bst2UI CCWGG 4 cut(s) 166, 357, 417, 1690
Bst4CI ACNGT 5 cut(s) 29, 320, 957, 1369, 1454
Bst6I CTCTTC 3 cut(s) 773, 1122, 1655
BstACI GRCGYC 4 cut(s) 102, 328, 981, 1475
BstAPI GCANNNNNTGC 1 cut(s) 1311
BstC8I GCNNGC 1 cut(s) 927
BstDEI CTNAG 3 cut(s) 18, 1222, 1772
BstDSI CCRYGG 2 cut(s) 798, 1440
BstF5I GGATG 6 cut(s) 12, 343, 434, 724, 979, 1140
BstH2I RGCGCY 2 cut(s) 105, 984
BstHHI GCGC 6 cut(s) 60, 75, 104, 316, 983, 1199
BstKTI GATC 9 cut(s) 153, 173, 211, 237, 391, 827, 1024, 1080, 1375
BstMAI GTCTC 1 cut(s) 1115
BstMBI GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
BstMWI GCNNNNNNNGC 8 cut(s) 79, 88, 405, 1258, 1311, 1502, 1511, 1597
BstNI CCWGG 4 cut(s) 166, 357, 417, 1690
BstSCI CCNGG 5 cut(s) 164, 355, 415, 896, 1688
BstSFI CTRYAG 2 cut(s) 406, 990
BstV1I GCAGC 5 cut(s) 26, 152, 383, 876, 1648
BstV2I GAAGAC 2 cut(s) 1297, 1448
BstX2I RGATCY 3 cut(s) 1021, 1077, 1372
BstXI CCANNNNNNTGG 2 cut(s) 249, 1048
BstYI RGATCY 3 cut(s) 1021, 1077, 1372
Bsu15I ATCGAT 1 cut(s) 823
BsuI GTATCC 3 cut(s) 836, 894, 924
BsuRI GGCC 7 cut(s) 184, 355, 768, 1514, 1640, 1748, 1758
BsuTUI ATCGAT 1 cut(s) 823
BtgI CCRYGG 2 cut(s) 798, 1440
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 6 cut(s) 12, 343, 434, 724, 979, 1140
BtsI GCAGTG 2 cut(s) 292, 415
BtsIMutI CAGTG 4 cut(s) 292, 415, 1035, 1374
BveI ACCTGC 1 cut(s) 1471
Cac8I GCNNGC 1 cut(s) 927
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 6 cut(s) 60, 75, 104, 316, 983, 1199
Cfr10I RCCGGY 1 cut(s) 1514
Cfr13I GGNCC 6 cut(s) 611, 701, 1154, 1209, 1747, 1757
ClaI ATCGAT 1 cut(s) 823
CseI GACGC 3 cut(s) 122, 132, 317
Csp6I GTAC 4 cut(s) 663, 832, 1487, 1767
CviAII CATG 9 cut(s) 461, 1042, 1082, 1148, 1194, 1302, 1381, 1433, 1655
CviQI GTAC 4 cut(s) 663, 832, 1487, 1767
DdeI CTNAG 3 cut(s) 18, 1222, 1772
DinI GGCGCC 2 cut(s) 103, 982
DpnI GATC 9 cut(s) 152, 172, 210, 236, 390, 826, 1023, 1079, 1374
DpnII GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
DraIII CACNNNGTG 1 cut(s) 1311
DrdI GACNNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 184
Eam1104I CTCTTC 3 cut(s) 773, 1122, 1655
EarI CTCTTC 3 cut(s) 773, 1122, 1655
EciI GGCGGA 2 cut(s) 275, 1615
Ecl136II GAGCTC 1 cut(s) 538
Eco130I CCWWGG 3 cut(s) 1157, 1506, 1539
Eco147I AGGCCT 1 cut(s) 768
Eco24I GRGCYC 2 cut(s) 540, 1240
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 4 cut(s) 611, 701, 1154, 1209
Eco53kI GAGCTC 1 cut(s) 538
EcoICRI GAGCTC 1 cut(s) 538
EcoO109I RGGNCCY 2 cut(s) 1154, 1209
EcoRI GAATTC 2 cut(s) 190, 253
EcoRII CCWGG 4 cut(s) 164, 355, 415, 1688
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 3 cut(s) 1157, 1506, 1539
EcoT38I GRGCYC 2 cut(s) 540, 1240
EgeI GGCGCC 2 cut(s) 103, 982
EheI GGCGCC 2 cut(s) 103, 982
ErhI CCWWGG 3 cut(s) 1157, 1506, 1539
Esp3I CGTCTC 1 cut(s) 1115
FaeI CATG 9 cut(s) 464, 1045, 1085, 1151, 1197, 1305, 1384, 1436, 1658
FalI AAGNNNNNCTT 2 cut(s) 524, 556
FaqI GGGAC 3 cut(s) 130, 303, 1167
FatI CATG 9 cut(s) 460, 1041, 1081, 1147, 1193, 1301, 1380, 1432, 1654
FauI CCCGC 2 cut(s) 1088, 1768
FauNDI CATATG 1 cut(s) 449
FblI GTMKAC 2 cut(s) 952, 1721
FokI GGATG 6 cut(s) 19, 350, 441, 731, 986, 1127
FriOI GRGCYC 2 cut(s) 540, 1240
FspAI RTGCGCAY 1 cut(s) 74
FspBI CTAG 5 cut(s) 753, 770, 926, 1287, 1520
FspI TGCGCA 2 cut(s) 74, 1198
GlaI GCGC 6 cut(s) 59, 74, 103, 315, 982, 1198
GsaI CCCAGC 2 cut(s) 130, 1704
GsuI CTGGAG 3 cut(s) 120, 319, 1481
HaeII RGCGCY 2 cut(s) 105, 984
HaeIII GGCC 7 cut(s) 184, 355, 768, 1514, 1640, 1748, 1758
HapII CCGG 6 cut(s) 99, 202, 704, 897, 1490, 1515
HgaI GACGC 3 cut(s) 122, 132, 317
HhaI GCGC 6 cut(s) 60, 75, 104, 316, 983, 1199
Hin1I GRCGYC 4 cut(s) 102, 328, 981, 1475
Hin1II CATG 9 cut(s) 464, 1045, 1085, 1151, 1197, 1305, 1384, 1436, 1658
Hin6I GCGC 6 cut(s) 58, 73, 102, 314, 981, 1197
HinP1I GCGC 6 cut(s) 58, 73, 102, 314, 981, 1197
HincII GTYRAC 2 cut(s) 953, 1722
HindII GTYRAC 2 cut(s) 953, 1722
HpaII CCGG 6 cut(s) 99, 202, 704, 897, 1490, 1515
HphI GGTGA 8 cut(s) 114, 244, 555, 584, 1027, 1072, 1346, 1408
Hpy166II GTNNAC 4 cut(s) 794, 953, 1337, 1722
Hpy188I TCNGA 8 cut(s) 333, 487, 829, 842, 918, 1186, 1360, 1738
Hpy188III TCNNGA 9 cut(s) 500, 685, 730, 849, 872, 1075, 1376, 1460, 1520
Hpy8I GTNNAC 4 cut(s) 794, 953, 1337, 1722
HpyAV CCTTC 9 cut(s) 16, 521, 578, 751, 1057, 1063, 1200, 1387, 1555
HpyCH4III ACNGT 5 cut(s) 29, 320, 957, 1369, 1454
HpyCH4IV ACGT 3 cut(s) 62, 644, 1475
HpyCH4V TGCA 6 cut(s) 341, 408, 544, 892, 1140, 1305
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 88, 405, 1258, 1311, 1502, 1511, 1597
HpyF3I CTNAG 3 cut(s) 18, 1222, 1772
HpySE526I ACGT 3 cut(s) 62, 644, 1475
Hsp92I GRCGYC 4 cut(s) 102, 328, 981, 1475
Hsp92II CATG 9 cut(s) 464, 1045, 1085, 1151, 1197, 1305, 1384, 1436, 1658
HspAI GCGC 6 cut(s) 58, 73, 102, 314, 981, 1197
KasI GGCGCC 2 cut(s) 101, 980
KpnI GGTACC 2 cut(s) 1490, 1770
Kzo9I GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
LguI GCTCTTC 2 cut(s) 1122, 1655
LmnI GCTCC 3 cut(s) 55, 87, 535
Lsp1109I GCAGC 5 cut(s) 26, 152, 383, 876, 1648
LweI GCATC 3 cut(s) 328, 865, 1149
MaeI CTAG 5 cut(s) 753, 770, 926, 1287, 1520
MaeII ACGT 3 cut(s) 62, 644, 1475
MaeIII GTNAC 5 cut(s) 443, 515, 640, 1363, 1445
MalI GATC 9 cut(s) 152, 172, 210, 236, 390, 826, 1023, 1079, 1374
MboI GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
MboII GAAGA 8 cut(s) 787, 790, 1139, 1302, 1448, 1544, 1585, 1642
MflI RGATCY 3 cut(s) 1021, 1077, 1372
MhlI GDGCHC 2 cut(s) 540, 1240
MluCI AATT 9 cut(s) 190, 253, 559, 629, 761, 1135, 1348, 1565, 1605
Mly113I GGCGCC 2 cut(s) 102, 981
MlyI GAGTC 8 cut(s) 127, 257, 659, 727, 861, 958, 1472, 1671
MmeI TCCRAC 2 cut(s) 311, 941
MseI TTAA 5 cut(s) 944, 1347, 1500, 1743, 1798
MslI CAYNNNNRTG 3 cut(s) 450, 1046, 1385
MspA1I CMGCKG 3 cut(s) 1095, 1469, 1763
MspI CCGG 6 cut(s) 99, 202, 704, 897, 1490, 1515
MspR9I CCNGG 5 cut(s) 166, 357, 417, 898, 1690
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 4 cut(s) 166, 357, 417, 1690
MwoI GCNNNNNNNGC 8 cut(s) 79, 88, 405, 1258, 1311, 1502, 1511, 1597
NarI GGCGCC 2 cut(s) 102, 981
NciI CCSGG 1 cut(s) 898
NdeI CATATG 1 cut(s) 449
NdeII GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
NheI GCTAGC 1 cut(s) 925
NlaIII CATG 9 cut(s) 464, 1045, 1085, 1151, 1197, 1305, 1384, 1436, 1658
NlaIV GGNNCC 6 cut(s) 103, 982, 1155, 1488, 1759, 1768
NmuCI GTSAC 2 cut(s) 443, 1363
NsbI TGCGCA 2 cut(s) 74, 1198
OliI CACNNNNGTG 1 cut(s) 450
PaqCI CACCTGC 1 cut(s) 1471
PceI AGGCCT 1 cut(s) 768
PciSI GCTCTTC 2 cut(s) 1122, 1655
PcsI WCGNNNNNNNCGW 3 cut(s) 185, 263, 380
PctI GAATGC 1 cut(s) 725
PfeI GAWTC 4 cut(s) 376, 1255, 1298, 1622
PflMI CCANNNNNTGG 2 cut(s) 620, 1689
PfoI TCCNGGA 1 cut(s) 896
PleI GAGTC 8 cut(s) 127, 257, 658, 727, 860, 957, 1471, 1671
PluTI GGCGCC 2 cut(s) 105, 984
PpsI GAGTC 8 cut(s) 127, 257, 658, 727, 860, 957, 1471, 1671
PpuMI RGGWCCY 2 cut(s) 1154, 1209
Psp124BI GAGCTC 1 cut(s) 540
Psp5II RGGWCCY 2 cut(s) 1154, 1209
Psp6I CCWGG 4 cut(s) 164, 355, 415, 1688
PspFI CCCAGC 2 cut(s) 126, 1700
PspGI CCWGG 4 cut(s) 164, 355, 415, 1688
PspN4I GGNNCC 6 cut(s) 103, 982, 1155, 1488, 1759, 1768
PspPI GGNCC 6 cut(s) 611, 701, 1154, 1209, 1747, 1757
PspPPI RGGWCCY 2 cut(s) 1154, 1209
PstI CTGCAG 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 3 cut(s) 1021, 1077, 1372
RsaI GTAC 4 cut(s) 664, 833, 1488, 1768
RsaNI GTAC 4 cut(s) 663, 832, 1487, 1767
RseI CAYNNNNRTG 3 cut(s) 450, 1046, 1385
SacI GAGCTC 1 cut(s) 540
SalI GTCGAC 2 cut(s) 951, 1720
SapI GCTCTTC 2 cut(s) 1122, 1655
SaqAI TTAA 5 cut(s) 944, 1347, 1500, 1743, 1798
Sau3AI GATC 9 cut(s) 150, 170, 208, 234, 388, 824, 1021, 1077, 1372
Sau96I GGNCC 6 cut(s) 611, 701, 1154, 1209, 1747, 1757
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 8 cut(s) 127, 257, 659, 727, 861, 958, 1472, 1671
ScrFI CCNGG 5 cut(s) 166, 357, 417, 898, 1690
SduI GDGCHC 2 cut(s) 540, 1240
SfaNI GCATC 3 cut(s) 328, 865, 1149
SfcI CTRYAG 2 cut(s) 406, 990
SfoI GGCGCC 2 cut(s) 103, 982
SinI GGWCC 4 cut(s) 611, 701, 1154, 1209
SmiMI CAYNNNNRTG 3 cut(s) 450, 1046, 1385
SmlI CTYRAG 3 cut(s) 510, 685, 847
SmoI CTYRAG 3 cut(s) 510, 685, 847
Sse9I AATT 9 cut(s) 190, 253, 559, 629, 761, 1135, 1348, 1565, 1605
SseBI AGGCCT 1 cut(s) 768
SspDI GGCGCC 2 cut(s) 101, 980
SspI AATATT 2 cut(s) 523, 1230
SspMI CTAG 5 cut(s) 753, 770, 926, 1287, 1520
SstI GAGCTC 1 cut(s) 540
StuI AGGCCT 1 cut(s) 768
StyD4I CCNGG 5 cut(s) 164, 355, 415, 896, 1688
StyI CCWWGG 3 cut(s) 1157, 1506, 1539
TaaI ACNGT 5 cut(s) 29, 320, 957, 1369, 1454
TaiI ACGT 3 cut(s) 65, 647, 1478
TaqI TCGA 8 cut(s) 188, 211, 736, 823, 952, 1218, 1593, 1721
TasI AATT 9 cut(s) 190, 253, 559, 629, 761, 1135, 1348, 1565, 1605
TatI WGTACW 1 cut(s) 662
TauI GCSGC 7 cut(s) 981, 1264, 1272, 1317, 1320, 1472, 1643
TfiI GAWTC 4 cut(s) 376, 1255, 1298, 1622
Tru1I TTAA 5 cut(s) 944, 1347, 1500, 1743, 1798
Tru9I TTAA 5 cut(s) 944, 1347, 1500, 1743, 1798
TscAI CASTG 4 cut(s) 299, 415, 1035, 1374
TseFI GTSAC 2 cut(s) 443, 1363
TseI GCWGC 5 cut(s) 14, 140, 396, 889, 1661
Tsp45I GTSAC 2 cut(s) 443, 1363
TspDTI ATGAA 6 cut(s) 449, 735, 853, 1233, 1397, 1671
TspGWI ACGGA 7 cut(s) 55, 57, 74, 350, 372, 1014, 1139
TspRI CASTG 4 cut(s) 299, 415, 1035, 1374
Van91I CCANNNNNTGG 2 cut(s) 620, 1689
VpaK11BI GGWCC 4 cut(s) 611, 701, 1154, 1209
XapI RAATTY 4 cut(s) 190, 253, 1565, 1605
XbaI TCTAGA 1 cut(s) 1519
XcmI CCANNNNNNNNNTGG 1 cut(s) 879
XmiI GTMKAC 2 cut(s) 952, 1721
XspI CTAG 5 cut(s) 753, 770, 926, 1287, 1520
ZraI GACGTC 1 cut(s) 1476
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.