MD12G1056300.v1.1

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013896
Physical Location & Seq
Reverse (-)
6410743 .. 6412038
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1056300.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGGAAAATCAGAAGCTGAAGTTTCATCACTTTAGCCACGAACATCCATTGGAGCGTACCAATGATTCACCTTCCAAACAGAACAAAATCTGTGCAGGGTGCAAGCTCATGATACAGTCCGGAAAGGATTACTACAGCTGTCCAAGCTGCCCATTTTTCTTGCACCAAGTATGCTACAACATGCCCCGGAAAACGCGTCACCCGGCTCTTCCAAGCCACTACTTGAACCTCCATCCAGCTCCTCCTTCAAGCACCAAGGGGGCCTTGAATTGTCAGGCTTGTGGAGATCCCGTCGAGGGGTTTTACTATGATTGTGCCGATTGTGGCTTTTACTATCATATCTTGTGCTCAGGCCTGCCCTTCTCCATTAAAACTTTATCCCACCCTCATGCATTGAAATTGTCATTTTCTCCACCGGTCGATTTGTTTTGTGATCTCTGCAATGAGCCTGGTTACGATCGATGGCTGTATAGGTGTCATATTTGTGAGTTTGATTCTCATATTTCTTGTGCCATTTATAATATTCAACCAGACCTTGAACCTCCCCAAAATCCAATTCCACCACTGCCCGCTGACACGTTTATAAGGCAGAGCAGCTATTCTTCAGGATCTTCTTTCGGGATCAATGTGGCAGAAAATTACAACAGTGAAGGCAATGAACTGTTGCATTTGGTAAGGCAGACAGTTTTTAAGGGTAACATTAAGTGCATTGGTGACGCAAAGGGTTTGAGTGCAGCAATAATTGGCTGGGATAAGAGATTGTGTTCTCCAAAACAAGTGCTCAACGTTAGAATGAGCAAACTTGGAAATGTTGGATCATATCAAACGGATGCAGATATGGCCGTCACTTCATTGAATTTGAAAGATACAGCAACTTCTGTTTCTGAAGACATGACTTTGACTCCATGTTATCAATTCAGTGATGCGTGTTTTTCGATAGATTTGTCTTATACACCTAATGGTCAGAAATTTCAAGCAAACAAGGAAGGTCCAAACCAAGGAACATTAAGCTACACCAATAGTACCATTGCCCCCAACAAGGTTTTGACCAGCGCCAAACCAGAAAAGCAACTCGGTTATGTTAATGTATCAGGTACTAATTATTCCAATAAAATTGGACCGGAAAATAAGCTGAATGAAGCATTCTTGACTCGAAATGACACTCTTACAAGGAAGGAATCAGGCCCAAAGGAGATGAAGAGGAAAGCTAGTGCTACCATGGGGAGCTCAACCATTGGAAATCAGAGTACTAAATCAGATATAGTAAGTCATAACGAATTTCGTACCATCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

432

Amino Acids

47.81

Weight (kDa)

8.01

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 11 - 58 1.4e-09 C1 domain
C1_2 PF03107 71 - 116 3.2e-08 C1 domain
C1_2 PF03107 127 - 171 2.3e-09 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42060
fragaria_vesca FvH4_6g05691 FvH4_6g22090
malus_domestica MD12G1056300.v1.1 MD14G1055700.v1.1
prunus_persica Prupe.7G078900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0479421
rosa_laevigata RLG00000023576
rosa_multiflora Rmu_sc0000107.1_g000006
rosa_roxburghii Rroxscaffold_6G00402290
rosa_rugosa Rorug03G0175200
rosa_samantha Rh3AG226500 Rh3BG259400 Rh3CG255300 Rh3DG250800 Rh3DG250900
rosa_wichuraiana Rw3G020200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 519, 584
AccII CGCG 1 cut(s) 196
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 1 cut(s) 570
AclI AACGTT 1 cut(s) 786
AclWI GGATC 4 cut(s) 281, 616, 629, 823
AcoI YGGCCR 1 cut(s) 840
AcsI RAATTY 3 cut(s) 856, 968, 1277
AcuI CTGAAG 3 cut(s) 38, 588, 906
AfaI GTAC 5 cut(s) 58, 1024, 1096, 1249, 1285
AfiI CCNNNNNNNGG 4 cut(s) 296, 297, 998, 1039
AflIII ACRYGT 2 cut(s) 194, 576
AgeI ACCGGT 1 cut(s) 415
AgsI TTSAA 9 cut(s) 226, 249, 268, 397, 527, 539, 856, 862, 974
AjnI CCWGG 1 cut(s) 448
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
Alw21I GWGCWC 3 cut(s) 350, 783, 1229
AlwI GGATC 4 cut(s) 281, 616, 629, 823
AlwNI CAGNNNCTG 1 cut(s) 16
Aor13HI TCCGGA 1 cut(s) 119
AoxI GGCC 4 cut(s) 261, 352, 840, 1183
ApeKI GCWGC 3 cut(s) 147, 594, 734
ApoI RAATTY 3 cut(s) 856, 968, 1277
AsiGI ACCGGT 1 cut(s) 415
AspLEI GCGC 1 cut(s) 1055
AspS9I GGNCC 4 cut(s) 261, 989, 1118, 1184
AsuC2I CCSGG 2 cut(s) 187, 203
AsuHPI GGTGA 3 cut(s) 60, 191, 725
AvaII GGWCC 2 cut(s) 989, 1118
BanII GRGCYC 1 cut(s) 1229
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 3 cut(s) 350, 783, 1229
BbvI GCAGC 3 cut(s) 134, 606, 746
BccI CCATC 3 cut(s) 240, 456, 1295
BceAI ACGGC 1 cut(s) 827
BciT130I CCWGG 1 cut(s) 450
BcnI CCSGG 2 cut(s) 187, 203
BfaI CTAG 1 cut(s) 1209
BfmI CTRYAG 1 cut(s) 133
BfoI RGCGCY 1 cut(s) 1056
BisI GCNGC 3 cut(s) 148, 595, 735
BlsI GCNGC 3 cut(s) 149, 596, 736
BmcAI AGTACT 1 cut(s) 1249
Bme1390I CCNGG 3 cut(s) 187, 203, 450
Bme18I GGWCC 2 cut(s) 989, 1118
BmgT120I GGNCC 4 cut(s) 261, 989, 1118, 1184
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 3 cut(s) 187, 203, 450
BmsI GCATC 2 cut(s) 820, 913
BpiI GAAGAC 1 cut(s) 894
Bpu10I CCTNAGC 1 cut(s) 349
BpuMI CCSGG 2 cut(s) 187, 203
Bsa29I ATCGAT 1 cut(s) 460
BsaJI CCNNGG 4 cut(s) 185, 255, 997, 1218
BsaWI WCCGGW 3 cut(s) 119, 415, 1120
BsaXI ACNNNNNCTCC 4 cut(s) 276, 306, 886, 916
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 297, 998, 1039
Bse118I RCCGGY 1 cut(s) 415
Bse3DI GCAATG 3 cut(s) 448, 661, 1026
BseAI TCCGGA 1 cut(s) 119
BseBI CCWGG 1 cut(s) 450
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 4 cut(s) 185, 255, 997, 1218
BseGI GGATG 3 cut(s) 43, 232, 835
BseLI CCNNNNNNNGG 4 cut(s) 296, 297, 998, 1039
BseMI GCAATG 3 cut(s) 448, 661, 1026
BseMII CTCAG 1 cut(s) 363
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 134, 606, 746
BseYI CCCAGC 1 cut(s) 747
BsgI GTGCAG 2 cut(s) 114, 753
Bsh1236I CGCG 1 cut(s) 196
Bsh1285I CGRYCG 2 cut(s) 420, 460
BshFI GGCC 4 cut(s) 263, 354, 842, 1185
BshTI ACCGGT 1 cut(s) 415
BshVI ATCGAT 1 cut(s) 460
BsiEI CGRYCG 2 cut(s) 420, 460
BsiHKAI GWGCWC 3 cut(s) 350, 783, 1229
BsiSI CCGG 5 cut(s) 120, 187, 203, 416, 1121
BslI CCNNNNNNNGG 4 cut(s) 296, 297, 998, 1039
BsmI GAATGC 1 cut(s) 1142
BsnI GGCC 4 cut(s) 263, 354, 842, 1185
Bsp1286I GDGCHC 3 cut(s) 350, 783, 1229
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 6 cut(s) 286, 433, 457, 608, 621, 815
Bsp19I CCATGG 1 cut(s) 1218
BspACI CCGC 1 cut(s) 570
BspANI GGCC 4 cut(s) 263, 354, 842, 1185
BspCNI CTCAG 1 cut(s) 362
BspDI ATCGAT 1 cut(s) 460
BspEI TCCGGA 1 cut(s) 119
BspFNI CGCG 1 cut(s) 196
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 262
BspPI GGATC 4 cut(s) 281, 616, 629, 823
BspQI GCTCTTC 1 cut(s) 213
BsrDI GCAATG 3 cut(s) 448, 661, 1026
BsrFI RCCGGY 1 cut(s) 415
BssAI RCCGGY 1 cut(s) 415
BssECI CCNNGG 4 cut(s) 185, 255, 997, 1218
BssMI GATC 6 cut(s) 286, 433, 457, 608, 621, 815
BssT1I CCWWGG 3 cut(s) 255, 997, 1218
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 4 cut(s) 117, 647, 663, 685
Bst6I CTCTTC 2 cut(s) 213, 1193
BstC8I GCNNGC 3 cut(s) 104, 356, 570
BstDEI CTNAG 1 cut(s) 349
BstDSI CCRYGG 1 cut(s) 1218
BstF5I GGATG 3 cut(s) 43, 232, 835
BstFNI CGCG 1 cut(s) 196
BstH2I RGCGCY 1 cut(s) 1056
BstHHI GCGC 1 cut(s) 1055
BstKTI GATC 6 cut(s) 289, 436, 460, 611, 624, 818
BstMBI GATC 6 cut(s) 286, 433, 457, 608, 621, 815
BstMCI CGRYCG 2 cut(s) 420, 460
BstMWI GCNNNNNNNGC 2 cut(s) 144, 839
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 3 cut(s) 185, 201, 448
BstSFI CTRYAG 1 cut(s) 133
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 3 cut(s) 134, 606, 746
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 2 cut(s) 286, 608
BstYI RGATCY 2 cut(s) 286, 608
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 4 cut(s) 263, 354, 842, 1185
BsuTUI ATCGAT 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 1218
BtsCI GGATG 3 cut(s) 43, 232, 835
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 3 cut(s) 563, 652, 925
Cac8I GCNNGC 3 cut(s) 104, 356, 570
CaiI CAGNNNCTG 1 cut(s) 16
CciI TCATGA 1 cut(s) 108
CfoI GCGC 1 cut(s) 1055
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 4 cut(s) 261, 989, 1118, 1184
ClaI ATCGAT 1 cut(s) 460
CseI GACGC 2 cut(s) 185, 725
Csp6I GTAC 5 cut(s) 57, 1023, 1095, 1248, 1284
CspAI ACCGGT 1 cut(s) 415
CviAII CATG 6 cut(s) 109, 181, 389, 892, 906, 1219
CviQI GTAC 5 cut(s) 57, 1023, 1095, 1248, 1284
DdeI CTNAG 1 cut(s) 349
DpnI GATC 6 cut(s) 288, 435, 459, 610, 623, 817
DpnII GATC 6 cut(s) 286, 433, 457, 608, 621, 815
EaeI YGGCCR 1 cut(s) 840
Eam1104I CTCTTC 2 cut(s) 213, 1193
EarI CTCTTC 2 cut(s) 213, 1193
Ecl136II GAGCTC 1 cut(s) 1227
Eco130I CCWWGG 3 cut(s) 255, 997, 1218
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 1229
Eco47I GGWCC 2 cut(s) 989, 1118
Eco53kI GAGCTC 1 cut(s) 1227
Eco57I CTGAAG 3 cut(s) 38, 588, 906
EcoICRI GAGCTC 1 cut(s) 1227
EcoO109I RGGNCCY 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 448
EcoT14I CCWWGG 3 cut(s) 255, 997, 1218
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 1229
ErhI CCWWGG 3 cut(s) 255, 997, 1218
FaeI CATG 6 cut(s) 112, 184, 392, 895, 909, 1222
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FatI CATG 6 cut(s) 108, 180, 388, 891, 905, 1218
FauI CCCGC 1 cut(s) 577
Fnu4HI GCNGC 3 cut(s) 148, 595, 735
FokI GGATG 3 cut(s) 30, 219, 842
FriOI GRGCYC 1 cut(s) 1229
Fsp4HI GCNGC 3 cut(s) 148, 595, 735
FspBI CTAG 1 cut(s) 1209
GlaI GCGC 1 cut(s) 1054
GluI GCNGC 3 cut(s) 148, 595, 735
GsaI CCCAGC 1 cut(s) 751
HaeII RGCGCY 1 cut(s) 1056
HaeIII GGCC 4 cut(s) 263, 354, 842, 1185
HapII CCGG 5 cut(s) 120, 187, 203, 416, 1121
HgaI GACGC 2 cut(s) 185, 725
HhaI GCGC 1 cut(s) 1055
Hin1II CATG 6 cut(s) 112, 184, 392, 895, 909, 1222
Hin6I GCGC 1 cut(s) 1053
HinP1I GCGC 1 cut(s) 1053
HinfI GANTC 5 cut(s) 65, 494, 901, 1150, 1178
HpaII CCGG 5 cut(s) 120, 187, 203, 416, 1121
HphI GGTGA 3 cut(s) 60, 191, 725
Hpy188I TCNGA 5 cut(s) 12, 886, 966, 1245, 1258
Hpy188III TCNNGA 5 cut(s) 109, 120, 606, 619, 1147
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 6 cut(s) 81, 255, 370, 644, 980, 1168
HpyCH4III ACNGT 4 cut(s) 117, 647, 663, 685
HpyCH4IV ACGT 2 cut(s) 578, 786
HpyCH4V TGCA 9 cut(s) 95, 102, 163, 392, 441, 667, 708, 734, 833
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 839
HpyF3I CTNAG 1 cut(s) 349
HpySE526I ACGT 2 cut(s) 578, 786
Hsp92II CATG 6 cut(s) 112, 184, 392, 895, 909, 1222
HspAI GCGC 1 cut(s) 1053
Kpn2I TCCGGA 1 cut(s) 119
Kzo9I GATC 6 cut(s) 286, 433, 457, 608, 621, 815
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 3 cut(s) 52, 244, 1224
Lsp1109I GCAGC 3 cut(s) 134, 606, 746
LweI GCATC 2 cut(s) 820, 913
MaeI CTAG 1 cut(s) 1209
MaeII ACGT 2 cut(s) 578, 786
MaeIII GTNAC 5 cut(s) 197, 452, 695, 713, 844
MalI GATC 6 cut(s) 288, 435, 459, 610, 623, 817
MboI GATC 6 cut(s) 286, 433, 457, 608, 621, 815
MboII GAAGA 5 cut(s) 200, 594, 603, 899, 1210
MfeI CAATTG 1 cut(s) 1291
MflI RGATCY 2 cut(s) 286, 608
MhlI GDGCHC 3 cut(s) 350, 783, 1229
MluI ACGCGT 1 cut(s) 194
MlyI GAGTC 2 cut(s) 895, 1144
MmeI TCCRAC 1 cut(s) 793
MnlI CCTC 6 cut(s) 239, 252, 289, 396, 552, 1194
Mph1103I ATGCAT 1 cut(s) 394
MroI TCCGGA 1 cut(s) 119
MseI TTAA 5 cut(s) 369, 690, 702, 1007, 1083
MslI CAYNNNNRTG 2 cut(s) 387, 483
MspA1I CMGCKG 2 cut(s) 138, 572
MspI CCGG 5 cut(s) 120, 187, 203, 416, 1121
MspR9I CCNGG 3 cut(s) 187, 203, 450
MunI CAATTG 1 cut(s) 1291
Mva1269I GAATGC 1 cut(s) 1142
MvaI CCWGG 1 cut(s) 450
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 144, 839
NciI CCSGG 2 cut(s) 187, 203
NcoI CCATGG 1 cut(s) 1218
NdeII GATC 6 cut(s) 286, 433, 457, 608, 621, 815
NlaIII CATG 6 cut(s) 112, 184, 392, 895, 909, 1222
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 197, 713, 844
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 1 cut(s) 184
PagI TCATGA 1 cut(s) 108
PceI AGGCCT 1 cut(s) 354
PciSI GCTCTTC 1 cut(s) 213
PctI GAATGC 1 cut(s) 1142
PfeI GAWTC 3 cut(s) 65, 494, 1178
PinAI ACCGGT 1 cut(s) 415
PkrI GCNGC 3 cut(s) 149, 596, 736
Ple19I CGATCG 1 cut(s) 460
PleI GAGTC 2 cut(s) 895, 1144
PpsI GAGTC 2 cut(s) 895, 1144
PsiI TTATAA 2 cut(s) 519, 584
Psp124BI GAGCTC 1 cut(s) 1229
Psp1406I AACGTT 1 cut(s) 786
Psp6I CCWGG 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 747
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 4 cut(s) 261, 989, 1118, 1184
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 2 cut(s) 286, 608
PvuI CGATCG 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 138
RsaI GTAC 5 cut(s) 58, 1024, 1096, 1249, 1285
RsaNI GTAC 5 cut(s) 57, 1023, 1095, 1248, 1284
RseI CAYNNNNRTG 2 cut(s) 387, 483
SacI GAGCTC 1 cut(s) 1229
SapI GCTCTTC 1 cut(s) 213
SaqAI TTAA 5 cut(s) 369, 690, 702, 1007, 1083
SatI GCNGC 3 cut(s) 148, 595, 735
Sau3AI GATC 6 cut(s) 286, 433, 457, 608, 621, 815
Sau96I GGNCC 4 cut(s) 261, 989, 1118, 1184
ScaI AGTACT 1 cut(s) 1249
SchI GAGTC 2 cut(s) 895, 1144
ScrFI CCNGG 3 cut(s) 187, 203, 450
SduI GDGCHC 3 cut(s) 350, 783, 1229
SfaNI GCATC 2 cut(s) 820, 913
SfcI CTRYAG 1 cut(s) 133
SinI GGWCC 2 cut(s) 989, 1118
SmiMI CAYNNNNRTG 2 cut(s) 387, 483
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 1 cut(s) 570
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 1209
SstI GAGCTC 1 cut(s) 1229
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 3 cut(s) 185, 201, 448
StyI CCWWGG 3 cut(s) 255, 997, 1218
TaaI ACNGT 4 cut(s) 117, 647, 663, 685
TaiI ACGT 2 cut(s) 581, 789
TaqI TCGA 5 cut(s) 294, 420, 460, 935, 1153
TatI WGTACW 1 cut(s) 1247
TfiI GAWTC 3 cut(s) 65, 494, 1178
Tru1I TTAA 5 cut(s) 369, 690, 702, 1007, 1083
Tru9I TTAA 5 cut(s) 369, 690, 702, 1007, 1083
TscAI CASTG 3 cut(s) 570, 652, 925
TseFI GTSAC 3 cut(s) 197, 713, 844
TseI GCWGC 3 cut(s) 147, 594, 734
Tsp45I GTSAC 3 cut(s) 197, 713, 844
TspDTI ATGAA 5 cut(s) 14, 672, 840, 1152, 1211
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 3 cut(s) 570, 652, 925
VpaK11BI GGWCC 2 cut(s) 989, 1118
XapI RAATTY 3 cut(s) 856, 968, 1277
XceI RCATGY 1 cut(s) 184
XspI CTAG 1 cut(s) 1209
ZrmI AGTACT 1 cut(s) 1249
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.