MD14G1055700.v1.1

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013896
Physical Location & Seq
Forward (+)
5674681 .. 5675979
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1055700.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGGAAAATCAGAAGTTGAAGTTTCATCACTTTAGCCATGAACATCCATTGGAGCTTACAAATTATTCACCTTCCAAACAGAACAAATTCTGTGCAGGGTGCAAGCTCATGATACAGTCCGGTAAGGACTACTACAGCTGCCCAACCTGCCCATTTTTATTGCACCAAGTATGCTACAGCATGCCCCGCAAGATGCGTCATCCGGCACATCCAAGCCACTACTTGAACCTCCATCCTTCTCCTCCTTCAAGCACCAAAGGGGCCTTGAATTGTCGGGCTTGTCAAACTCATGTCATGGGGTTTTACTATGATTGTGCCGATTGTGGCTTTTACTATCATAGCTTGTGCTCGGGCCTGCCCTTCTCCATTAAAACTTCATCGCACCCTCATGCATTGAAATTATCATTTTCTCCACCGTTCGATTTGAGCTGTGATCTCTGCAATGAGTCTGGTTATGATCAATGGCTGTATAGGTGTCACATTTGTGAGTTTGATTCTCATATTTCTTGTGCCATTTATAATATGCAACCAGGTCTTGAACCTACCCAAAATCCAATTCCCCCACCGCCCGCCAACGGGTTTACAAGGCAGAGCAGCTATTCTTCAGGAGCTTTTTTCGGGATCAATGAGGCAGAAAATTACAACAGTGAAGGCAATGAACTCTTGCATTTGGTAAGACAGATAGTTTTTAAGGGTAACATTAAAAGCATTGGTGACAAAAAGGTTTCAAGTGCAGCAGTCACCGGCTGGGATAAGAGATTAATTTCTCCGAAACAAGAGTTCAACTTTACAACTAGCAAACTTGGAAATGGTGGATCACATCAAACGGATGCAGACATGGCCATCACTTCTTTGAATTCAAAAGATACAGCAACTTCGGTTTCTGAAGACATTACATTGACTCCAAGTTATCAATTCAGCGATGCGTATTTTTCGATAGATTTGGCGAAGTCCACATCTAATGGTCAGAAAATTCAAGCAAACAGGGAAGGTCCAAACCAAGGAACATTAAGCTACACCGACAGTACTATTCCCCCCAAGAAGGTTTTGAGCAGCGCCAAACCAGCAAAGCAACCCAATTATGTTAATGTATCAGGTACTAATTATTCCATTAAAATTGGACCGGAAAATAAGCTGAATGAAGCATTCTTGACTCGAAATGACACTCTTACAAGGAAGGAATCAGGCCCAAAGGAGATGAAGAGAAAAGCTAGTGATACCATGGGGAGCTCAGACACCGGAAATCAGAGTCCTAAATCAGATATGGTAAGTGATACCGAATTTCATACCATCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2

Protein Analysis

433

Amino Acids

47.92

Weight (kDa)

8.08

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C1_2 PF03107 11 - 58 1.6e-10 C1 domain
C1_2 PF03107 69 - 116 3.2e-07 C1 domain
C1_2 PF03107 127 - 171 1.4e-08 C1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013896)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42060
fragaria_vesca FvH4_6g05691 FvH4_6g22090
malus_domestica MD12G1056300.v1.1 MD14G1055700.v1.1
prunus_persica Prupe.7G078900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0479421
rosa_laevigata RLG00000023576
rosa_multiflora Rmu_sc0000107.1_g000006
rosa_roxburghii Rroxscaffold_6G00402290
rosa_rugosa Rorug03G0175200
rosa_samantha Rh3AG226500 Rh3BG259400 Rh3CG255300 Rh3DG250800 Rh3DG250900
rosa_wichuraiana Rw3G020200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 155
AciI CCGC 3 cut(s) 187, 566, 570
AclWI GGATC 2 cut(s) 629, 823
AcoI YGGCCR 1 cut(s) 840
AcsI RAATTY 4 cut(s) 86, 856, 972, 1280
AcuI CTGAAG 2 cut(s) 588, 906
AfaI GTAC 2 cut(s) 1027, 1099
AfiI CCNNNNNNNGG 4 cut(s) 575, 576, 1001, 1042
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AleI CACNNNNGTG 1 cut(s) 483
Alw21I GWGCWC 2 cut(s) 350, 1232
AlwI GGATC 2 cut(s) 629, 823
Ama87I CYCGRG 1 cut(s) 349
AoxI GGCC 4 cut(s) 261, 352, 840, 1186
ApeKI GCWGC 4 cut(s) 138, 594, 734, 1053
ApoI RAATTY 4 cut(s) 86, 856, 972, 1280
AseI ATTAAT 1 cut(s) 761
Asp700I GAANNNNTTC 1 cut(s) 86
AspLEI GCGC 1 cut(s) 1058
AspS9I GGNCC 5 cut(s) 261, 352, 992, 1121, 1187
AsuHPI GGTGA 3 cut(s) 60, 725, 733
AvaI CYCGRG 1 cut(s) 349
AvaII GGWCC 2 cut(s) 992, 1121
BalI TGGCCA 1 cut(s) 842
BanII GRGCYC 1 cut(s) 1232
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 2 cut(s) 350, 1232
BbvI GCAGC 4 cut(s) 125, 606, 746, 1065
BccI CCATC 3 cut(s) 240, 851, 1298
BciT130I CCWGG 1 cut(s) 531
BclI TGATCA 1 cut(s) 457
BfaI CTAG 2 cut(s) 795, 1212
BfmI CTRYAG 2 cut(s) 133, 175
BfoI RGCGCY 1 cut(s) 1059
BfuAI ACCTGC 1 cut(s) 155
BisI GCNGC 4 cut(s) 139, 595, 735, 1054
BlsI GCNGC 4 cut(s) 140, 596, 736, 1055
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 1 cut(s) 531
Bme18I GGWCC 2 cut(s) 992, 1121
BmeT110I CYCGRG 1 cut(s) 349
BmgT120I GGNCC 5 cut(s) 261, 352, 992, 1121, 1187
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 531
BmsI GCATC 3 cut(s) 183, 820, 913
BpiI GAAGAC 1 cut(s) 894
BsaJI CCNNGG 2 cut(s) 1000, 1221
BsaWI WCCGGW 3 cut(s) 119, 1123, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 886, 916
Bsc4I CCNNNNNNNGG 4 cut(s) 575, 576, 1001, 1042
Bse118I RCCGGY 1 cut(s) 743
Bse3DI GCAATG 2 cut(s) 448, 661
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 1000, 1221
BseGI GGATG 5 cut(s) 43, 199, 208, 232, 835
BseLI CCNNNNNNNGG 4 cut(s) 575, 576, 1001, 1042
BseMI GCAATG 2 cut(s) 448, 661
BseMII CTCAG 1 cut(s) 1245
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 4 cut(s) 125, 606, 746, 1065
BseYI CCCAGC 1 cut(s) 747
BsgI GTGCAG 2 cut(s) 114, 753
BshFI GGCC 4 cut(s) 263, 354, 842, 1188
BsiHKAI GWGCWC 2 cut(s) 350, 1232
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 5 cut(s) 120, 203, 744, 1124, 1239
BslI CCNNNNNNNGG 4 cut(s) 575, 576, 1001, 1042
BsmI GAATGC 1 cut(s) 1145
BsnI GGCC 4 cut(s) 263, 354, 842, 1188
BsoBI CYCGRG 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 350, 1232
Bsp143I GATC 4 cut(s) 433, 457, 621, 815
Bsp19I CCATGG 1 cut(s) 1221
BspACI CCGC 3 cut(s) 187, 566, 570
BspANI GGCC 4 cut(s) 263, 354, 842, 1188
BspCNI CTCAG 1 cut(s) 1244
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 262
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 2 cut(s) 629, 823
BsrDI GCAATG 2 cut(s) 448, 661
BsrFI RCCGGY 1 cut(s) 743
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 2 cut(s) 1000, 1221
BssMI GATC 4 cut(s) 433, 457, 621, 815
BssT1I CCWWGG 2 cut(s) 1000, 1221
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 4 cut(s) 117, 417, 647, 1025
Bst6I CTCTTC 1 cut(s) 1196
BstC8I GCNNGC 4 cut(s) 104, 182, 356, 570
BstDEI CTNAG 1 cut(s) 1231
BstDSI CCRYGG 1 cut(s) 1221
BstF5I GGATG 5 cut(s) 43, 199, 208, 232, 835
BstH2I RGCGCY 1 cut(s) 1059
BstHHI GCGC 1 cut(s) 1058
BstKTI GATC 4 cut(s) 436, 460, 624, 818
BstMBI GATC 4 cut(s) 433, 457, 621, 815
BstMWI GCNNNNNNNGC 4 cut(s) 147, 186, 839, 1064
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 529
BstSFI CTRYAG 2 cut(s) 133, 175
BstV1I GCAGC 4 cut(s) 125, 606, 746, 1065
BstV2I GAAGAC 1 cut(s) 894
BsuRI GGCC 4 cut(s) 263, 354, 842, 1188
BtgI CCRYGG 1 cut(s) 1221
BtgZI GCGATG 2 cut(s) 363, 936
BtsCI GGATG 5 cut(s) 43, 199, 208, 232, 835
BtsIMutI CAGTG 1 cut(s) 652
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 4 cut(s) 104, 182, 356, 570
CciI TCATGA 1 cut(s) 108
CfoI GCGC 1 cut(s) 1058
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 5 cut(s) 261, 352, 992, 1121, 1187
CseI GACGC 1 cut(s) 185
CsiI ACCWGGT 1 cut(s) 529
Csp6I GTAC 2 cut(s) 1026, 1098
CviAII CATG 8 cut(s) 38, 109, 181, 290, 295, 389, 838, 1222
CviQI GTAC 2 cut(s) 1026, 1098
DdeI CTNAG 1 cut(s) 1231
DpnI GATC 4 cut(s) 435, 459, 623, 817
DpnII GATC 4 cut(s) 433, 457, 621, 815
EaeI YGGCCR 1 cut(s) 840
Eam1104I CTCTTC 1 cut(s) 1196
EarI CTCTTC 1 cut(s) 1196
Ecl136II GAGCTC 1 cut(s) 1230
Eco130I CCWWGG 2 cut(s) 1000, 1221
Eco24I GRGCYC 1 cut(s) 1232
Eco47I GGWCC 2 cut(s) 992, 1121
Eco53kI GAGCTC 1 cut(s) 1230
Eco57I CTGAAG 2 cut(s) 588, 906
Eco88I CYCGRG 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 1230
EcoO109I RGGNCCY 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 856
EcoRII CCWGG 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 1000, 1221
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 1232
ErhI CCWWGG 2 cut(s) 1000, 1221
FaeI CATG 8 cut(s) 41, 112, 184, 293, 298, 392, 841, 1225
FatI CATG 8 cut(s) 37, 108, 180, 289, 294, 388, 837, 1221
FauI CCCGC 2 cut(s) 194, 577
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 4 cut(s) 139, 595, 735, 1054
FokI GGATG 5 cut(s) 30, 186, 195, 219, 842
FriOI GRGCYC 1 cut(s) 1232
Fsp4HI GCNGC 4 cut(s) 139, 595, 735, 1054
FspBI CTAG 2 cut(s) 795, 1212
GlaI GCGC 1 cut(s) 1057
GluI GCNGC 4 cut(s) 139, 595, 735, 1054
GsaI CCCAGC 1 cut(s) 751
HaeII RGCGCY 1 cut(s) 1059
HaeIII GGCC 4 cut(s) 263, 354, 842, 1188
HapII CCGG 5 cut(s) 120, 203, 744, 1124, 1239
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 1058
Hin1II CATG 8 cut(s) 41, 112, 184, 293, 298, 392, 841, 1225
Hin6I GCGC 1 cut(s) 1056
HinP1I GCGC 1 cut(s) 1056
HinfI GANTC 6 cut(s) 446, 494, 901, 1153, 1181, 1249
HpaII CCGG 5 cut(s) 120, 203, 744, 1124, 1239
HphI GGTGA 3 cut(s) 60, 725, 733
Hpy166II GTNNAC 2 cut(s) 582, 954
Hpy188I TCNGA 7 cut(s) 12, 771, 886, 969, 1234, 1248, 1261
Hpy188III TCNNGA 5 cut(s) 109, 536, 606, 619, 1150
Hpy8I GTNNAC 2 cut(s) 582, 954
HpyAV CCTTC 8 cut(s) 81, 246, 255, 370, 644, 983, 1036, 1171
HpyCH4III ACNGT 4 cut(s) 117, 417, 647, 1025
HpyCH4V TGCA 9 cut(s) 95, 102, 163, 392, 441, 526, 667, 734, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 186, 839, 1064
HpyF3I CTNAG 1 cut(s) 1231
Hsp92II CATG 8 cut(s) 41, 112, 184, 293, 298, 392, 841, 1225
HspAI GCGC 1 cut(s) 1056
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 4 cut(s) 433, 457, 621, 815
LmnI GCTCC 3 cut(s) 52, 608, 1227
Lsp1109I GCAGC 4 cut(s) 125, 606, 746, 1065
LweI GCATC 3 cut(s) 183, 820, 913
MabI ACCWGGT 1 cut(s) 529
MaeI CTAG 2 cut(s) 795, 1212
MaeIII GTNAC 4 cut(s) 476, 695, 713, 739
MalI GATC 4 cut(s) 435, 459, 623, 817
MboI GATC 4 cut(s) 433, 457, 621, 815
MboII GAAGA 3 cut(s) 594, 899, 1213
MfeI CAATTG 1 cut(s) 1294
MhlI GDGCHC 2 cut(s) 350, 1232
MlsI TGGCCA 1 cut(s) 842
MluNI TGGCCA 1 cut(s) 842
MlyI GAGTC 4 cut(s) 455, 895, 1147, 1258
MnlI CCTC 4 cut(s) 239, 252, 396, 622
Mox20I TGGCCA 1 cut(s) 842
Mph1103I ATGCAT 1 cut(s) 394
MroXI GAANNNNTTC 1 cut(s) 86
MscI TGGCCA 1 cut(s) 842
MseI TTAA 7 cut(s) 369, 690, 702, 761, 1010, 1086, 1113
MslI CAYNNNNRTG 2 cut(s) 387, 483
Msp20I TGGCCA 1 cut(s) 842
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 5 cut(s) 120, 203, 744, 1124, 1239
MspR9I CCNGG 1 cut(s) 531
MunI CAATTG 1 cut(s) 1294
Mva1269I GAATGC 1 cut(s) 1145
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 4 cut(s) 147, 186, 839, 1064
NcoI CCATGG 1 cut(s) 1221
NdeII GATC 4 cut(s) 433, 457, 621, 815
NlaIII CATG 8 cut(s) 41, 112, 184, 293, 298, 392, 841, 1225
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 476, 713, 739
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 1 cut(s) 184
OliI CACNNNNGTG 1 cut(s) 483
PaeI GCATGC 1 cut(s) 184
PagI TCATGA 1 cut(s) 108
PctI GAATGC 1 cut(s) 1145
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 2 cut(s) 494, 1181
PkrI GCNGC 4 cut(s) 140, 596, 736, 1055
PleI GAGTC 4 cut(s) 454, 895, 1147, 1257
PpsI GAGTC 4 cut(s) 454, 895, 1147, 1257
PshBI ATTAAT 1 cut(s) 761
PsiI TTATAA 1 cut(s) 519
Psp124BI GAGCTC 1 cut(s) 1232
Psp6I CCWGG 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 747
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 5 cut(s) 261, 352, 992, 1121, 1187
PvuII CAGCTG 1 cut(s) 138
RsaI GTAC 2 cut(s) 1027, 1099
RsaNI GTAC 2 cut(s) 1026, 1098
RseI CAYNNNNRTG 2 cut(s) 387, 483
SacI GAGCTC 1 cut(s) 1232
SaqAI TTAA 7 cut(s) 369, 690, 702, 761, 1010, 1086, 1113
SatI GCNGC 4 cut(s) 139, 595, 735, 1054
Sau3AI GATC 4 cut(s) 433, 457, 621, 815
Sau96I GGNCC 5 cut(s) 261, 352, 992, 1121, 1187
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 4 cut(s) 455, 895, 1147, 1258
ScrFI CCNGG 1 cut(s) 531
SduI GDGCHC 2 cut(s) 350, 1232
SexAI ACCWGGT 1 cut(s) 529
SfaNI GCATC 3 cut(s) 183, 820, 913
SfcI CTRYAG 2 cut(s) 133, 175
SinI GGWCC 2 cut(s) 992, 1121
SmiMI CAYNNNNRTG 2 cut(s) 387, 483
SphI GCATGC 1 cut(s) 184
SsiI CCGC 3 cut(s) 187, 566, 570
SspMI CTAG 2 cut(s) 795, 1212
SstI GAGCTC 1 cut(s) 1232
StyD4I CCNGG 1 cut(s) 529
StyI CCWWGG 2 cut(s) 1000, 1221
TaaI ACNGT 4 cut(s) 117, 417, 647, 1025
TaqI TCGA 3 cut(s) 420, 935, 1156
TatI WGTACW 1 cut(s) 1025
TfiI GAWTC 2 cut(s) 494, 1181
Tru1I TTAA 7 cut(s) 369, 690, 702, 761, 1010, 1086, 1113
Tru9I TTAA 7 cut(s) 369, 690, 702, 761, 1010, 1086, 1113
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 3 cut(s) 476, 713, 739
TseI GCWGC 4 cut(s) 138, 594, 734, 1053
Tsp45I GTSAC 3 cut(s) 476, 713, 739
TspDTI ATGAA 7 cut(s) 14, 54, 366, 672, 1155, 1214, 1274
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 1 cut(s) 652
VpaK11BI GGWCC 2 cut(s) 992, 1121
VspI ATTAAT 1 cut(s) 761
XapI RAATTY 4 cut(s) 86, 856, 972, 1280
XceI RCATGY 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 2 cut(s) 795, 1212
ZrmI AGTACT 1 cut(s) 1027
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.