FvH4_6g07050

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005594
Physical Location & Seq
Reverse (-)
4240056 .. 4243960
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07050.t1

Sequence Viewer

Length: 1281 bp
ATGAGTGAGACTGAAGTAGCAAGGATGAAATCTCCTCTTGTTCCACTTGCAACTTTGCTTGGGCGTGAGATTAGGAGCGAGAAGGTTGAGGAACCCTTTTTGAAGTATGGGCAGGCTGCTTTGGCAAAGAAAGGAGAGGATTATTTTCTGATAAAACCAGATTGCCGTAGAATTCATGCGAATCCTTCAACCTCATTTTCTGTTTTTGCGATATTTGATGGGCATAATGGTATATCAGCTGCTATTTATGCAAAGGAGACTTTATTGGATAATGTTTTGAGTGCAATTCCTCAAGGTCTCACTCGGGACGAATGGCTTCAAGCCCTGCCTCGGGCACTGGTTGCTGGTTTTGTGAAAACTGATATAGAATTTCAGCAGAAAGGGGAAACATCTGGTACAACTGTGACGTTTGTTGTGATTGATGGTTGGACTGTGACTGTTGCATCTGTTGGGGATTCTAGATGCATATTAGACACCCAGGGAGGTGTGGTTTCACTATTGACTGTAGATCACAGGCTGGAAGAAAATGAAGAAGAGAGGGAGCGTGTTACAGCTAGTGGGGGTGAAGTAGGAAGGCTCAATGTCTTTGGGGGTAGTGAGGTTGGCCCCCTACGCTGCTGGCCTGGTGGGTTATGCCTTTCTAGATCAATTGGTGACACAGATGTGGGTGAATATATTGTCCCAGTACCACATGTGAAGCAAGTAAAGCTTTCAAATGCCGGGGGAAGACTTATAATAGCTTCTGATGGCATCTGGGATGCTCTGTCTTCTGAAATGGCTGCACAGTCTTGTCGCGGATTGCCTGCAGAGCTTGCTGCAAAGCTGGTCGTGAAGGAGGCTCTGAGGTCAAGGGGTCTGAAAGATGATACTACCTGCCTAGTTGTTGACATAATCCCATCAGAACGTCCTGTCTTGCCTCCAACTCCAAGGAAGAAACAAAACATGCTCACTTCACTTATTTTTGGGAAGAAAAGTCAGAATGGGAGCAAAACAAATAAGCTTTCAGCGGTGGGAGTTGTGGAGGAACTGTTTGAAGAAGGTTCAGCAATGCTCGCTGAAAGGTTGGGTAAAGATATTCCTTTGGATGCAAATTCTGGGATCTTCCGGTGTGCAGTTTGCCAAGTGGATCAGCCCCAATCAGAAGGTTTATCAGTAAATTCTGGACCTTTCTTCTCACCATCATCAAAGCCATGGGAAGGCCCTTTCCTCTGCGCAGTCTGTCGGAGAAAGAAAGATGCCATGGAAGGAAAGAGAATGAGCACACCCACAGTGGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

427

Amino Acids

45.97

Weight (kDa)

5.99

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 61 - 280 1.4e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 734
Acc16I TGCGCA 1 cut(s) 1213
Acc36I ACCTGC 1 cut(s) 881
AccII CGCG 1 cut(s) 795
AciI CCGC 2 cut(s) 795, 1007
AclWI GGATC 2 cut(s) 1106, 1134
AcsI RAATTY 4 cut(s) 171, 368, 1090, 1156
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 2 cut(s) 397, 687
AfiI CCNNNNNNNGG 3 cut(s) 330, 331, 1196
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 5 cut(s) 103, 189, 320, 714, 1034
AjnI CCWGG 2 cut(s) 477, 622
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 7 cut(s) 239, 554, 709, 740, 811, 823, 1000
AluI AGCT 7 cut(s) 239, 554, 709, 740, 811, 823, 1000
Alw21I GWGCWC 1 cut(s) 1262
Alw26I GTCTC 3 cut(s) 2, 251, 302
AlwI GGATC 2 cut(s) 1106, 1134
Ama87I CYCGRG 2 cut(s) 303, 330
AoxI GGCC 4 cut(s) 604, 620, 1198, 1272
ApeKI GCWGC 5 cut(s) 116, 239, 615, 779, 815
ApoI RAATTY 4 cut(s) 171, 368, 1090, 1156
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 1214
AspS9I GGNCC 3 cut(s) 605, 1163, 1199
AsuC2I CCSGG 1 cut(s) 721
AsuHPI GGTGA 4 cut(s) 575, 665, 680, 1167
AvaI CYCGRG 2 cut(s) 303, 330
AvaII GGWCC 1 cut(s) 1163
BaeGI GKGCMC 1 cut(s) 337
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BbsI GAAGAC 2 cut(s) 733, 759
Bbv12I GWGCWC 1 cut(s) 1262
BbvI GCAGC 5 cut(s) 103, 226, 602, 766, 802
BccI CCATC 5 cut(s) 212, 416, 740, 904, 1186
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 2 cut(s) 479, 624
BcnI CCSGG 1 cut(s) 721
BcoDI GTCTC 3 cut(s) 2, 251, 302
BfaI CTAG 4 cut(s) 459, 555, 642, 878
BfmI CTRYAG 2 cut(s) 504, 804
BfuAI ACCTGC 1 cut(s) 881
BisI GCNGC 5 cut(s) 117, 240, 616, 780, 816
BlsI GCNGC 5 cut(s) 118, 241, 617, 781, 817
Bme1390I CCNGG 3 cut(s) 479, 624, 721
Bme18I GGWCC 1 cut(s) 1163
BmeT110I CYCGRG 2 cut(s) 303, 330
BmgT120I GGNCC 3 cut(s) 605, 1163, 1199
BmiI GGNNCC 2 cut(s) 93, 607
BmrFI CCNGG 3 cut(s) 479, 624, 721
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 6 cut(s) 452, 452, 748, 759, 1075, 1225
BmuI ACTGGG 1 cut(s) 677
BpiI GAAGAC 2 cut(s) 733, 759
BpuEI CTTGAG 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 721
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 7 cut(s) 329, 477, 478, 720, 926, 1190, 1239
BsaWI WCCGGW 1 cut(s) 1104
BsaXI ACNNNNNCTCC 4 cut(s) 474, 504, 1013, 1043
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 331, 1196
Bse1I ACTGG 2 cut(s) 342, 683
Bse3DI GCAATG 1 cut(s) 1053
BseBI CCWGG 2 cut(s) 479, 624
BseDI CCNNGG 7 cut(s) 329, 477, 478, 720, 926, 1190, 1239
BseGI GGATG 3 cut(s) 30, 763, 1090
BseLI CCNNNNNNNGG 3 cut(s) 330, 331, 1196
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 1 cut(s) 833
BseNI ACTGG 2 cut(s) 342, 683
BseRI GAGGAG 1 cut(s) 24
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 5 cut(s) 103, 226, 602, 766, 802
BsgI GTGCAG 2 cut(s) 765, 1131
Bsh1236I CGCG 1 cut(s) 795
BshFI GGCC 4 cut(s) 606, 622, 1200, 1274
BsiHKAI GWGCWC 1 cut(s) 1262
BsiHKCI CYCGRG 2 cut(s) 303, 330
BsiSI CCGG 2 cut(s) 720, 1105
BslFI GGGAC 2 cut(s) 320, 665
BslI CCNNNNNNNGG 3 cut(s) 330, 331, 1196
BsmAI GTCTC 3 cut(s) 2, 251, 302
BsmFI GGGAC 2 cut(s) 320, 665
BsnI GGCC 4 cut(s) 606, 622, 1200, 1274
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 2 cut(s) 303, 330
Bsp1286I GDGCHC 2 cut(s) 337, 1262
Bsp143I GATC 4 cut(s) 508, 644, 1098, 1126
Bsp19I CCATGG 2 cut(s) 1190, 1239
BspACI CCGC 2 cut(s) 795, 1007
BspANI GGCC 4 cut(s) 606, 622, 1200, 1274
BspCNI CTCAG 1 cut(s) 834
BspFNI CGCG 1 cut(s) 795
BspLI GGNNCC 2 cut(s) 93, 607
BspMAI CTGCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 2 cut(s) 1106, 1134
BspTNI GGTCTC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 1053
BsrI ACTGG 2 cut(s) 342, 683
BssECI CCNNGG 7 cut(s) 329, 477, 478, 720, 926, 1190, 1239
BssMI GATC 4 cut(s) 508, 644, 1098, 1126
BssT1I CCWWGG 3 cut(s) 926, 1190, 1239
Bst2UI CCWGG 2 cut(s) 479, 624
Bst4CI ACNGT 7 cut(s) 403, 433, 439, 505, 786, 1029, 1270
Bst6I CTCTTC 1 cut(s) 528
BstAPI GCANNNNNTGC 2 cut(s) 341, 812
BstC8I GCNNGC 5 cut(s) 114, 620, 804, 813, 1053
BstDEI CTNAG 1 cut(s) 842
BstDSI CCRYGG 2 cut(s) 1190, 1239
BstF5I GGATG 3 cut(s) 30, 763, 1090
BstFNI CGCG 1 cut(s) 795
BstHHI GCGC 1 cut(s) 1214
BstKTI GATC 4 cut(s) 511, 647, 1101, 1129
BstMAI GTCTC 3 cut(s) 2, 251, 302
BstMBI GATC 4 cut(s) 508, 644, 1098, 1126
BstMWI GCNNNNNNNGC 8 cut(s) 122, 248, 341, 612, 706, 808, 812, 1052
BstNI CCWGG 2 cut(s) 479, 624
BstNSI RCATGY 2 cut(s) 695, 946
BstSCI CCNGG 3 cut(s) 477, 622, 719
BstSFI CTRYAG 2 cut(s) 504, 804
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 1 cut(s) 795
BstV1I GCAGC 5 cut(s) 103, 226, 602, 766, 802
BstV2I GAAGAC 2 cut(s) 733, 759
BstX2I RGATCY 1 cut(s) 1098
BstYI RGATCY 1 cut(s) 1098
BsuRI GGCC 4 cut(s) 606, 622, 1200, 1274
BtgI CCRYGG 2 cut(s) 1190, 1239
BtsCI GGATG 3 cut(s) 30, 763, 1090
BtsIMutI CAGTG 2 cut(s) 335, 1275
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 5 cut(s) 114, 620, 804, 813, 1053
CfoI GCGC 1 cut(s) 1214
Cfr13I GGNCC 3 cut(s) 605, 1163, 1199
Csp6I GTAC 2 cut(s) 396, 686
CviAII CATG 5 cut(s) 176, 692, 943, 1191, 1240
CviQI GTAC 2 cut(s) 396, 686
DdeI CTNAG 1 cut(s) 842
DpnI GATC 4 cut(s) 510, 646, 1100, 1128
DpnII GATC 4 cut(s) 508, 644, 1098, 1126
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 3 cut(s) 926, 1190, 1239
Eco31I GGTCTC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 1163
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 2 cut(s) 303, 330
EcoO109I RGGNCCY 1 cut(s) 1199
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 477, 622
EcoT14I CCWWGG 3 cut(s) 926, 1190, 1239
EcoT22I ATGCAT 1 cut(s) 467
ErhI CCWWGG 3 cut(s) 926, 1190, 1239
FaeI CATG 5 cut(s) 179, 695, 946, 1194, 1243
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FaqI GGGAC 2 cut(s) 320, 665
FatI CATG 5 cut(s) 175, 691, 942, 1190, 1239
Fnu4HI GCNGC 5 cut(s) 117, 240, 616, 780, 816
FokI GGATG 3 cut(s) 37, 770, 1097
Fsp4HI GCNGC 5 cut(s) 117, 240, 616, 780, 816
FspBI CTAG 4 cut(s) 459, 555, 642, 878
FspI TGCGCA 1 cut(s) 1213
GlaI GCGC 1 cut(s) 1213
GluI GCNGC 5 cut(s) 117, 240, 616, 780, 816
HaeIII GGCC 4 cut(s) 606, 622, 1200, 1274
HapII CCGG 2 cut(s) 720, 1105
HhaI GCGC 1 cut(s) 1214
Hin1II CATG 5 cut(s) 179, 695, 946, 1194, 1243
Hin6I GCGC 1 cut(s) 1212
HinP1I GCGC 1 cut(s) 1212
HincII GTYRAC 1 cut(s) 886
HindII GTYRAC 1 cut(s) 886
HindIII AAGCTT 2 cut(s) 707, 998
HinfI GANTC 2 cut(s) 181, 455
HpaII CCGG 2 cut(s) 720, 1105
HphI GGTGA 4 cut(s) 575, 665, 680, 1167
Hpy166II GTNNAC 1 cut(s) 886
Hpy188I TCNGA 9 cut(s) 150, 745, 772, 843, 858, 901, 978, 1141, 1224
Hpy188III TCNNGA 5 cut(s) 305, 459, 642, 829, 1161
Hpy8I GTNNAC 1 cut(s) 886
HpyAV CCTTC 8 cut(s) 76, 195, 567, 826, 1031, 1136, 1190, 1238
HpyCH4III ACNGT 7 cut(s) 403, 433, 439, 505, 786, 1029, 1270
HpyCH4IV ACGT 2 cut(s) 407, 904
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 248, 341, 612, 706, 808, 812, 1052
HpyF3I CTNAG 1 cut(s) 842
HpySE526I ACGT 2 cut(s) 407, 904
Hsp92II CATG 5 cut(s) 179, 695, 946, 1194, 1243
HspAI GCGC 1 cut(s) 1212
Kzo9I GATC 4 cut(s) 508, 644, 1098, 1126
LmnI GCTCC 3 cut(s) 75, 541, 984
Lsp1109I GCAGC 5 cut(s) 103, 226, 602, 766, 802
LweI GCATC 6 cut(s) 452, 452, 748, 759, 1075, 1225
MaeI CTAG 4 cut(s) 459, 555, 642, 878
MaeII ACGT 2 cut(s) 407, 904
MaeIII GTNAC 4 cut(s) 403, 433, 547, 653
MalI GATC 4 cut(s) 510, 646, 1100, 1128
MboI GATC 4 cut(s) 508, 644, 1098, 1126
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 1098
MhlI GDGCHC 2 cut(s) 337, 1262
MluCI AATT 6 cut(s) 171, 285, 368, 648, 1090, 1156
MmeI TCCRAC 3 cut(s) 407, 944, 1202
Mph1103I ATGCAT 1 cut(s) 467
MroXI GAANNNNTTC 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 2 cut(s) 239, 1007
MspI CCGG 2 cut(s) 720, 1105
MspR9I CCNGG 3 cut(s) 479, 624, 721
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 2 cut(s) 479, 624
MvnI CGCG 1 cut(s) 795
MwoI GCNNNNNNNGC 8 cut(s) 122, 248, 341, 612, 706, 808, 812, 1052
NciI CCSGG 1 cut(s) 721
NcoI CCATGG 2 cut(s) 1190, 1239
NdeII GATC 4 cut(s) 508, 644, 1098, 1126
NlaIII CATG 5 cut(s) 179, 695, 946, 1194, 1243
NlaIV GGNNCC 2 cut(s) 93, 607
NmuCI GTSAC 3 cut(s) 403, 433, 653
NsbI TGCGCA 1 cut(s) 1213
NsiI ATGCAT 1 cut(s) 467
NspI RCATGY 2 cut(s) 695, 946
OliI CACNNNNGTG 1 cut(s) 662
PasI CCCWGGG 1 cut(s) 478
PciI ACATGT 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 181, 455
PkrI GCNGC 5 cut(s) 118, 241, 617, 781, 817
PscI ACATGT 1 cut(s) 691
PsiI TTATAA 1 cut(s) 734
Psp6I CCWGG 2 cut(s) 477, 622
PspGI CCWGG 2 cut(s) 477, 622
PspN4I GGNNCC 2 cut(s) 93, 607
PspPI GGNCC 3 cut(s) 605, 1163, 1199
PstI CTGCAG 1 cut(s) 808
PsuI RGATCY 1 cut(s) 1098
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 397, 687
RsaNI GTAC 2 cut(s) 396, 686
RseI CAYNNNNRTG 1 cut(s) 662
SatI GCNGC 5 cut(s) 117, 240, 616, 780, 816
Sau3AI GATC 4 cut(s) 508, 644, 1098, 1126
Sau96I GGNCC 3 cut(s) 605, 1163, 1199
ScrFI CCNGG 3 cut(s) 479, 624, 721
SduI GDGCHC 2 cut(s) 337, 1262
SfaNI GCATC 6 cut(s) 452, 452, 748, 759, 1075, 1225
SfcI CTRYAG 2 cut(s) 504, 804
SinI GGWCC 1 cut(s) 1163
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
Sse9I AATT 6 cut(s) 171, 285, 368, 648, 1090, 1156
SsiI CCGC 2 cut(s) 795, 1007
SspMI CTAG 4 cut(s) 459, 555, 642, 878
StyD4I CCNGG 3 cut(s) 477, 622, 719
StyI CCWWGG 3 cut(s) 926, 1190, 1239
TaaI ACNGT 7 cut(s) 403, 433, 439, 505, 786, 1029, 1270
TaiI ACGT 2 cut(s) 410, 907
TasI AATT 6 cut(s) 171, 285, 368, 648, 1090, 1156
TfiI GAWTC 2 cut(s) 181, 455
TscAI CASTG 2 cut(s) 342, 1275
TseFI GTSAC 3 cut(s) 403, 433, 653
TseI GCWGC 5 cut(s) 116, 239, 615, 779, 815
Tsp45I GTSAC 3 cut(s) 403, 433, 653
TspDTI ATGAA 3 cut(s) 41, 164, 543
TspRI CASTG 2 cut(s) 342, 1275
VpaK11BI GGWCC 1 cut(s) 1163
XapI RAATTY 4 cut(s) 171, 368, 1090, 1156
XbaI TCTAGA 2 cut(s) 458, 641
XceI RCATGY 2 cut(s) 695, 946
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 4 cut(s) 459, 555, 642, 878
Zsp2I ATGCAT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.