Rh3DG078500

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005594
Physical Location & Seq
Reverse (-)
6086501 .. 6103016
16516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG078500.1

Sequence Viewer

Length: 1332 bp
ATGACTTTTCGGTTTTGGCACTTGTCTCTTGAAAATGTCACAAGGACTAAAATGAGTCAGACTGAAGTAGCGAGGATGAAGTCTCCTCTTGTTCCACTTGCAACTTTGATTGGGCGTGAGATTAGGGGCGAGAAGGTTCAGGAACCCTATTTAAAGTATGGGCAGGCTGCTTTGGCAAAGAAAGGAGAGGATTATTTTCTGATAAAACCAGATTGCCAAAGAATTCATGCTAATCCTTCAACCTCATTTTCTGTTTTTGCGATATTTGATGGGCATAATGGTAACTCAGCTGCTATTTATGCAAAGGAGACTTTATTGGATAATGTTTTGAGTGCAATTCCTCAAGGTAGCAGTAGGGACGAATGGCTTCAAGCCCTGCCTCGGGCACTGGTTGCCGGTTTTGTGAAAACTGATATAGAATTTCAGCAGAAAGGGGAAACATCTGGTACAACTGTTACATTCGTTGTAATTGATGGTTGGACTGTGACTGTGGCATCTGTTGGGGATTCTAGATGCATATTAGACACCCAGGGAGGTGTGGTTTCACTATTGACTGTTGATCACAGGCTGGAAGAAAATGAAGAGGAGAGGGAGCGTGTGACAGCTAGTGGGGGTGAAGTAGGAAGGCTTAATGTCTTTGGGGGCAGTGAGGTCGGTCCCCTACGCTGCTGGCCTGGTGGGTTATGCCTTTCTAGGTCAATTGGTGACACAGACGTGGGTGAATTTATTGTCCCAGTACCACATGTCAAGCAAGTAAAGCTTTCAAATGCCGGGGGAAGACTTATAATAGCTTCTGATGGCATCTGGGATGCTCTGTCTTCTGATATGGCTGCCAAGTCTTGTCGGGGATTGCCTGCAGAGCTTGCTGCAAAGTTGGTTGTTAAGGAGGCTCTGAGGTCAAGGGGTCTGAAAGATGATACAACCTGCGTAGTTGTTGACATAATCCCATCAGACCGTCCTGTCTTGCCTCCAACTCCGAGGAAGAAACAAAGCATGCTCACTTCACTTATTTTTGGGAAGAAAAGTCAGAATGGGAGCAAAACAAATAAGCTTTCAGCGGTGGGAGTTGTGGAGGAACTGTTTGAAGAAGGTTCAGCAATGCTCGCTGAAAGGTTGGGTAAGGATATTCCTTTGGATGCAAATTCTGGTATTTTCCGGTGTGCAGTCTGCCAAGTTGATCAGCCCCAATCAGAAGGTTTATCAGTAAATTCTGCACCTTTTTTCTCATCATCATCAACGCCATGGGAAGGCCCCTTCCTCTGCACAACCTGTCGGAAAAAGAAAGACGCCATGGAAGGAAAAAGAATGAGCACACCCACAGTGGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

443

Amino Acids

47.93

Weight (kDa)

6.96

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 78 - 297 2.8e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 785
AasI GACNNNNNNGTC 1 cut(s) 959
Acc36I ACCTGC 1 cut(s) 932
AciI CCGC 1 cut(s) 1058
AcsI RAATTY 5 cut(s) 222, 419, 722, 1141, 1207
AcuI CTGAAG 1 cut(s) 84
AcyI GRCGYC 1 cut(s) 1287
AfaI GTAC 2 cut(s) 448, 738
AfiI CCNNNNNNNGG 3 cut(s) 381, 382, 1247
AflIII ACRYGT 1 cut(s) 742
AgsI TTSAA 5 cut(s) 32, 240, 371, 765, 1085
AjiI CACGTC 1 cut(s) 715
AjnI CCWGG 2 cut(s) 528, 673
AleI CACNNNNGTG 1 cut(s) 713
AluBI AGCT 6 cut(s) 290, 605, 760, 791, 862, 1051
AluI AGCT 6 cut(s) 290, 605, 760, 791, 862, 1051
Alw21I GWGCWC 1 cut(s) 1313
Alw26I GTCTC 3 cut(s) 30, 87, 302
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 2 cut(s) 671, 1249
ApeKI GCWGC 5 cut(s) 167, 290, 666, 830, 866
ApoI RAATTY 5 cut(s) 222, 419, 722, 1141, 1207
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 656, 1250
AsuC2I CCSGG 1 cut(s) 772
AsuHPI GGTGA 3 cut(s) 626, 716, 731
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 388
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BbsI GAAGAC 2 cut(s) 784, 810
Bbv12I GWGCWC 1 cut(s) 1313
BbvI GCAGC 5 cut(s) 154, 277, 653, 817, 853
BccI CCATC 4 cut(s) 263, 467, 791, 955
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciT130I CCWGG 2 cut(s) 530, 675
BclI TGATCA 2 cut(s) 559, 1177
BcnI CCSGG 1 cut(s) 772
BcoDI GTCTC 3 cut(s) 30, 87, 302
BfaI CTAG 3 cut(s) 510, 606, 693
BfmI CTRYAG 1 cut(s) 855
BfuAI ACCTGC 1 cut(s) 932
BisI GCNGC 5 cut(s) 168, 291, 667, 831, 867
BlsI GCNGC 5 cut(s) 169, 292, 668, 832, 868
Bme1390I CCNGG 3 cut(s) 530, 675, 772
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 715
BmgT120I GGNCC 2 cut(s) 656, 1250
BmiI GGNNCC 3 cut(s) 144, 658, 1252
BmrFI CCNGG 3 cut(s) 530, 675, 772
BmrI ACTGGG 1 cut(s) 728
BmsI GCATC 5 cut(s) 503, 503, 799, 810, 1126
BmuI ACTGGG 1 cut(s) 728
BpiI GAAGAC 2 cut(s) 784, 810
BpuEI CTTGAG 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 772
BsaHI GRCGYC 1 cut(s) 1287
BsaJI CCNNGG 7 cut(s) 380, 528, 529, 771, 977, 1241, 1290
BsaWI WCCGGW 1 cut(s) 1155
BsaXI ACNNNNNCTCC 4 cut(s) 525, 555, 1064, 1094
Bsc4I CCNNNNNNNGG 3 cut(s) 381, 382, 1247
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 2 cut(s) 393, 734
Bse3DI GCAATG 1 cut(s) 1104
BseBI CCWGG 2 cut(s) 530, 675
BseDI CCNNGG 7 cut(s) 380, 528, 529, 771, 977, 1241, 1290
BseGI GGATG 3 cut(s) 81, 814, 1141
BseLI CCNNNNNNNGG 3 cut(s) 381, 382, 1247
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 2 cut(s) 300, 884
BseNI ACTGG 2 cut(s) 393, 734
BseRI GAGGAG 2 cut(s) 75, 599
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 5 cut(s) 154, 277, 653, 817, 853
BsgI GTGCAG 3 cut(s) 1182, 1197, 1246
BshFI GGCC 2 cut(s) 673, 1251
BsiHKAI GWGCWC 1 cut(s) 1313
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 3 cut(s) 396, 771, 1156
BslFI GGGAC 3 cut(s) 371, 642, 716
BslI CCNNNNNNNGG 3 cut(s) 381, 382, 1247
BsmAI GTCTC 3 cut(s) 30, 87, 302
BsmFI GGGAC 3 cut(s) 371, 642, 716
BsnI GGCC 2 cut(s) 673, 1251
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 388, 1313
Bsp143I GATC 2 cut(s) 559, 1177
Bsp19I CCATGG 2 cut(s) 1241, 1290
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 2 cut(s) 673, 1251
BspCNI CTCAG 2 cut(s) 299, 885
BspLI GGNNCC 3 cut(s) 144, 658, 1252
BspMAI CTGCAG 1 cut(s) 859
BspMI ACCTGC 1 cut(s) 932
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 2 cut(s) 393, 734
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 7 cut(s) 380, 528, 529, 771, 977, 1241, 1290
BssMI GATC 2 cut(s) 559, 1177
BssNI GRCGYC 1 cut(s) 1287
BssT1I CCWWGG 2 cut(s) 1241, 1290
Bst2UI CCWGG 2 cut(s) 530, 675
Bst4CI ACNGT 7 cut(s) 454, 484, 490, 556, 956, 1080, 1321
Bst6I CTCTTC 1 cut(s) 576
BstACI GRCGYC 1 cut(s) 1287
BstAPI GCANNNNNTGC 2 cut(s) 392, 863
BstC8I GCNNGC 6 cut(s) 165, 671, 855, 864, 995, 1104
BstDEI CTNAG 2 cut(s) 286, 893
BstDSI CCRYGG 2 cut(s) 1241, 1290
BstF5I GGATG 3 cut(s) 81, 814, 1141
BstKTI GATC 2 cut(s) 562, 1180
BstMAI GTCTC 3 cut(s) 30, 87, 302
BstMBI GATC 2 cut(s) 559, 1177
BstMWI GCNNNNNNNGC 7 cut(s) 173, 299, 392, 757, 859, 863, 1103
BstNI CCWGG 2 cut(s) 530, 675
BstNSI RCATGY 2 cut(s) 746, 997
BstSCI CCNGG 3 cut(s) 528, 673, 770
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 5 cut(s) 154, 277, 653, 817, 853
BstV2I GAAGAC 2 cut(s) 784, 810
BsuRI GGCC 2 cut(s) 673, 1251
BtgI CCRYGG 2 cut(s) 1241, 1290
BtrI CACGTC 1 cut(s) 715
BtsCI GGATG 3 cut(s) 81, 814, 1141
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 3 cut(s) 386, 652, 1326
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 6 cut(s) 165, 671, 855, 864, 995, 1104
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 656, 1250
CseI GACGC 1 cut(s) 1295
Csp6I GTAC 2 cut(s) 447, 737
CviAII CATG 5 cut(s) 227, 743, 994, 1242, 1291
CviQI GTAC 2 cut(s) 447, 737
DdeI CTNAG 2 cut(s) 286, 893
DpnI GATC 2 cut(s) 561, 1179
DpnII GATC 2 cut(s) 559, 1177
DraI TTTAAA 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Eco130I CCWWGG 2 cut(s) 1241, 1290
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 528, 673
EcoT14I CCWWGG 2 cut(s) 1241, 1290
EcoT22I ATGCAT 1 cut(s) 518
ErhI CCWWGG 2 cut(s) 1241, 1290
FaeI CATG 5 cut(s) 230, 746, 997, 1245, 1294
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 3 cut(s) 371, 642, 716
FatI CATG 5 cut(s) 226, 742, 993, 1241, 1290
FbaI TGATCA 2 cut(s) 559, 1177
Fnu4HI GCNGC 5 cut(s) 168, 291, 667, 831, 867
FokI GGATG 3 cut(s) 88, 821, 1148
Fsp4HI GCNGC 5 cut(s) 168, 291, 667, 831, 867
FspBI CTAG 3 cut(s) 510, 606, 693
GluI GCNGC 5 cut(s) 168, 291, 667, 831, 867
HaeIII GGCC 2 cut(s) 673, 1251
HapII CCGG 3 cut(s) 396, 771, 1156
HgaI GACGC 1 cut(s) 1295
Hin1I GRCGYC 1 cut(s) 1287
Hin1II CATG 5 cut(s) 230, 746, 997, 1245, 1294
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HindIII AAGCTT 2 cut(s) 758, 1049
HinfI GANTC 2 cut(s) 55, 506
HpaII CCGG 3 cut(s) 396, 771, 1156
HphI GGTGA 3 cut(s) 626, 716, 731
Hpy166II GTNNAC 1 cut(s) 937
Hpy188III TCNNGA 3 cut(s) 29, 140, 510
Hpy8I GTNNAC 1 cut(s) 937
HpyAV CCTTC 8 cut(s) 127, 246, 618, 1082, 1187, 1241, 1264, 1289
HpyCH4III ACNGT 7 cut(s) 454, 484, 490, 556, 956, 1080, 1321
HpyCH4IV ACGT 1 cut(s) 714
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 299, 392, 757, 859, 863, 1103
HpyF3I CTNAG 2 cut(s) 286, 893
HpySE526I ACGT 1 cut(s) 714
Hsp92I GRCGYC 1 cut(s) 1287
Hsp92II CATG 5 cut(s) 230, 746, 997, 1245, 1294
Ksp22I TGATCA 2 cut(s) 559, 1177
Kzo9I GATC 2 cut(s) 559, 1177
LmnI GCTCC 2 cut(s) 592, 1035
Lsp1109I GCAGC 5 cut(s) 154, 277, 653, 817, 853
LweI GCATC 5 cut(s) 503, 503, 799, 810, 1126
MaeI CTAG 3 cut(s) 510, 606, 693
MaeII ACGT 1 cut(s) 714
MaeIII GTNAC 6 cut(s) 37, 281, 454, 484, 598, 704
MalI GATC 2 cut(s) 561, 1179
MboI GATC 2 cut(s) 559, 1177
MboII GAAGA 7 cut(s) 584, 593, 789, 810, 994, 1030, 1097
MfeI CAATTG 1 cut(s) 699
MhlI GDGCHC 2 cut(s) 388, 1313
MluCI AATT 8 cut(s) 222, 336, 419, 468, 699, 722, 1141, 1207
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 3 cut(s) 458, 995, 1253
Mph1103I ATGCAT 1 cut(s) 518
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 3 cut(s) 152, 630, 882
MslI CAYNNNNRTG 1 cut(s) 713
MspA1I CMGCKG 2 cut(s) 290, 1058
MspI CCGG 3 cut(s) 396, 771, 1156
MspR9I CCNGG 3 cut(s) 530, 675, 772
MunI CAATTG 1 cut(s) 699
MvaI CCWGG 2 cut(s) 530, 675
MwoI GCNNNNNNNGC 7 cut(s) 173, 299, 392, 757, 859, 863, 1103
NciI CCSGG 1 cut(s) 772
NcoI CCATGG 2 cut(s) 1241, 1290
NdeII GATC 2 cut(s) 559, 1177
NlaIII CATG 5 cut(s) 230, 746, 997, 1245, 1294
NlaIV GGNNCC 3 cut(s) 144, 658, 1252
NmuCI GTSAC 4 cut(s) 37, 484, 598, 704
NsiI ATGCAT 1 cut(s) 518
NspI RCATGY 2 cut(s) 746, 997
OliI CACNNNNGTG 1 cut(s) 713
PaeI GCATGC 1 cut(s) 997
PasI CCCWGGG 1 cut(s) 529
PciI ACATGT 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 506
PkrI GCNGC 5 cut(s) 169, 292, 668, 832, 868
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PscI ACATGT 1 cut(s) 742
PsiI TTATAA 1 cut(s) 785
Psp6I CCWGG 2 cut(s) 528, 673
PspGI CCWGG 2 cut(s) 528, 673
PspN4I GGNNCC 3 cut(s) 144, 658, 1252
PspPI GGNCC 2 cut(s) 656, 1250
PstI CTGCAG 1 cut(s) 859
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 448, 738
RsaNI GTAC 2 cut(s) 447, 737
RseI CAYNNNNRTG 1 cut(s) 713
SaqAI TTAA 3 cut(s) 152, 630, 882
SatI GCNGC 5 cut(s) 168, 291, 667, 831, 867
Sau3AI GATC 2 cut(s) 559, 1177
Sau96I GGNCC 2 cut(s) 656, 1250
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 3 cut(s) 530, 675, 772
SduI GDGCHC 2 cut(s) 388, 1313
SfaNI GCATC 5 cut(s) 503, 503, 799, 810, 1126
SfcI CTRYAG 1 cut(s) 855
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 713
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
SphI GCATGC 1 cut(s) 997
Sse9I AATT 8 cut(s) 222, 336, 419, 468, 699, 722, 1141, 1207
SsiI CCGC 1 cut(s) 1058
SspMI CTAG 3 cut(s) 510, 606, 693
StyD4I CCNGG 3 cut(s) 528, 673, 770
StyI CCWWGG 2 cut(s) 1241, 1290
TaaI ACNGT 7 cut(s) 454, 484, 490, 556, 956, 1080, 1321
TaiI ACGT 1 cut(s) 717
TaqII GACCGA 1 cut(s) 644
TasI AATT 8 cut(s) 222, 336, 419, 468, 699, 722, 1141, 1207
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 3 cut(s) 152, 630, 882
Tru9I TTAA 3 cut(s) 152, 630, 882
TscAI CASTG 3 cut(s) 393, 652, 1326
TseFI GTSAC 4 cut(s) 37, 484, 598, 704
TseI GCWGC 5 cut(s) 167, 290, 666, 830, 866
Tsp45I GTSAC 4 cut(s) 37, 484, 598, 704
TspDTI ATGAA 3 cut(s) 92, 215, 594
TspRI CASTG 3 cut(s) 393, 652, 1326
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 5 cut(s) 222, 419, 722, 1141, 1207
XbaI TCTAGA 1 cut(s) 509
XceI RCATGY 2 cut(s) 746, 997
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 3 cut(s) 510, 606, 693
Zsp2I ATGCAT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.