MD04G1196500.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005594
Physical Location & Seq
Forward (+)
28486255 .. 28489718
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1196500.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGACTTCAATGTCAGATCCTCCCTGCTCGATCCGTCTCTGGATGCCAGTGTTTTTGCTCTGCATTCAAAGGTACTAAATTTCAAAAATTATACTGGCAAGAACAACCTTTTCTTTTTGCCCCTTCGGTATGAATTCTGGTTTTGGCAATTGGGTCCTGGAAATGTCTCAAGGGACACTAAAATGAGCCAGATTGAAGTATCGGCAATGAAATCTTCTCCCATCCCACTTGCGACTCTGATTGGTCAGGAGCTTAGGGGTGAAGAAGTTGAGAAACCTTATTTGAAGTATGGGCAGGCTGGTTTGGCGAAGAAAGGAGAAGATTACTTTCTTATAAAAACTGACTGCCAAAGAATTCATTCGGATCCGTCAACAACATTTTCTGTCTTTGCGATTTTTGATGGGCATAATGGTATATCAGCTGCTATTTTTACAAAGGAGAATTTATTGGCTAATGTTTTGAGTGCCATTCCGCAAGGAATCAGTAGGGATGAATGGCTGCTTGCCCTTCCTCGGGCGCTGGTTGCAGGATTTGTCAAAACTGATATAGAATTTCAGCAGAAGGGGGAAACTTCTGGTACAACTGTGACTTTTGTTGTGATCGATGGTTGGACTGTGACTGCTGCATCTGTTGGGGATTCTCGATGCATATTGGACACCCAGGGAGGTGTTGTTTCACTCTTGACTGTTGATCACAGGCTGGAAGAAAATGAAGAAGAGAGGGAGCGAGTTACTGCCAGTGGGGGCGAAGTAGGAAGGTTAAATGTTTTTGGGGGCAATGAGGTTGGTCCCCTCCGCTGCTGGCCTGGTGGATTGTGCCTTTCTAGGTCAATTGGTGACACAGATGTGGGAGAATACATTGTCCCAATACCACATGTCAAGCAAGTGAAGCTTTCAAATGCTGGGGGAAGACTTATAATAGCTTCTGATGGCATCTGGGATGCTTTGTCTTCTGATATGGCGGCGAAGTCATGTCGAGGATTGCCTGCAGAGCTTGCTGCGAAGATGGTCGTTAAGGAGGCTCTGAGGTCAAGGGGACTGAAAGATGATACAACCTGCGTAGTTGTTGACATAATCCCTTCAGGTCATCCTGTCTTACCTCCAACACCGATGAAGAAACCAAACATGCTTGCTTCACTTATCTTTGGGAAGAAAAGTCAGAATTCTGTGAACAAAACAAATAAGCTTTCTGCTATCGGAGTTGTGGAAGAGTTATTTGAAGAAGGTTCAGCAATGCTCGATGAAAGGTTGGGGAAGGATTCTCCTGTGGATGCAAATTCTGGGATTTTCCGATGCGCAGTCTGCCAAGTGGATCAGCCCCCAGTAGAAGGTTTATCAGTAAACTCTGGGCCTTTCTTTTCCCCAGCATCAAAGCCATGGGAAGGCCCCTTCCTCTGCGCAAAGTGTCAGACCAAGAAAGACGCCATGGAAGGGAAAAGACTAACCAGACCTACTGTGGTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

489

Amino Acids

52.92

Weight (kDa)

5.99

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 122 - 342 6.1e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 335, 917
AasI GACNNNNNNGTC 1 cut(s) 11
Acc16I TGCGCA 2 cut(s) 1297, 1399
Acc36I ACCTGC 1 cut(s) 1064
AciI CCGC 3 cut(s) 473, 796, 962
AclWI GGATC 5 cut(s) 12, 26, 359, 372, 1320
AcsI RAATTY 7 cut(s) 79, 134, 354, 442, 551, 1162, 1276
AcuI CTGAAG 1 cut(s) 1065
AcyI GRCGYC 1 cut(s) 1422
AfaI GTAC 2 cut(s) 75, 580
AfiI CCNNNNNNNGG 5 cut(s) 513, 514, 1328, 1382, 1431
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 7 cut(s) 10, 69, 85, 197, 286, 897, 1220
AjnI CCWGG 3 cut(s) 157, 660, 805
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 6 cut(s) 253, 422, 892, 923, 994, 1186
AluI AGCT 6 cut(s) 253, 422, 892, 923, 994, 1186
Alw26I GTCTC 2 cut(s) 42, 172
AlwI GGATC 5 cut(s) 12, 26, 359, 372, 1320
Ama87I CYCGRG 1 cut(s) 513
AoxI GGCC 3 cut(s) 803, 1349, 1384
ApeKI GCWGC 5 cut(s) 422, 499, 623, 798, 998
ApoI RAATTY 7 cut(s) 79, 134, 354, 442, 551, 1162, 1276
Asp700I GAANNNNTTC 1 cut(s) 358
AspLEI GCGC 3 cut(s) 520, 1298, 1400
AspS9I GGNCC 4 cut(s) 155, 788, 1349, 1385
AsuHPI GGTGA 2 cut(s) 272, 848
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 2 cut(s) 155, 788
BaeI ACNNNNGTAYC 2 cut(s) 570, 603
BamHI GGATCC 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 916, 942
BbvI GCAGC 5 cut(s) 409, 486, 610, 785, 985
BccI CCATC 5 cut(s) 230, 395, 599, 923, 1000
BciT130I CCWGG 3 cut(s) 159, 662, 807
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 42, 172
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 987
BfoI RGCGCY 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 1064
BisI GCNGC 6 cut(s) 423, 500, 624, 799, 963, 999
BlsI GCNGC 6 cut(s) 424, 501, 625, 800, 964, 1000
Bme1390I CCNGG 3 cut(s) 159, 662, 807
Bme18I GGWCC 2 cut(s) 155, 788
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 4 cut(s) 155, 788, 1349, 1385
BmiI GGNNCC 4 cut(s) 156, 366, 790, 1387
BmrFI CCNGG 3 cut(s) 159, 662, 807
BmrI ACTGGG 1 cut(s) 1316
BmsI GCATC 8 cut(s) 34, 635, 635, 931, 942, 1261, 1283, 1376
BmuI ACTGGG 1 cut(s) 1316
BpiI GAAGAC 2 cut(s) 916, 942
Bpu10I CCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 154
Bsa29I ATCGAT 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 1422
BsaJI CCNNGG 5 cut(s) 512, 660, 661, 1376, 1425
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 5 cut(s) 513, 514, 1328, 1382, 1431
Bse1I ACTGG 4 cut(s) 48, 100, 738, 1322
Bse3DI GCAATG 3 cut(s) 213, 784, 1239
BseBI CCWGG 3 cut(s) 159, 662, 807
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 5 cut(s) 512, 660, 661, 1376, 1425
BseGI GGATG 6 cut(s) 49, 222, 496, 946, 1087, 1276
BseLI CCNNNNNNNGG 5 cut(s) 513, 514, 1328, 1382, 1431
BseMI GCAATG 3 cut(s) 213, 784, 1239
BseMII CTCAG 1 cut(s) 1016
BseNI ACTGG 4 cut(s) 48, 100, 738, 1322
BseXI GCAGC 5 cut(s) 409, 486, 610, 785, 985
BseYI CCCAGC 2 cut(s) 902, 1363
BshFI GGCC 3 cut(s) 805, 1351, 1386
BshVI ATCGAT 1 cut(s) 603
BsiHKCI CYCGRG 1 cut(s) 513
BslFI GGGAC 4 cut(s) 188, 774, 848, 1050
BslI CCNNNNNNNGG 5 cut(s) 513, 514, 1328, 1382, 1431
BsmAI GTCTC 2 cut(s) 42, 172
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 4 cut(s) 188, 774, 848, 1050
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 3 cut(s) 805, 1351, 1386
BsoBI CYCGRG 1 cut(s) 513
Bsp143I GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
Bsp19I CCATGG 2 cut(s) 1376, 1425
BspACI CCGC 3 cut(s) 473, 796, 962
BspANI GGCC 3 cut(s) 805, 1351, 1386
BspCNI CTCAG 1 cut(s) 1017
BspDI ATCGAT 1 cut(s) 603
BspLI GGNNCC 4 cut(s) 156, 366, 790, 1387
BspMAI CTGCAG 1 cut(s) 991
BspMI ACCTGC 1 cut(s) 1064
BspPI GGATC 5 cut(s) 12, 26, 359, 372, 1320
BsrDI GCAATG 3 cut(s) 213, 784, 1239
BsrI ACTGG 4 cut(s) 48, 100, 738, 1322
BssECI CCNNGG 5 cut(s) 512, 660, 661, 1376, 1425
BssMI GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
BssNI GRCGYC 1 cut(s) 1422
BssT1I CCWWGG 2 cut(s) 1376, 1425
Bst2UI CCWGG 3 cut(s) 159, 662, 807
Bst4CI ACNGT 4 cut(s) 586, 616, 688, 1456
Bst6I CTCTTC 2 cut(s) 711, 1203
BstACI GRCGYC 1 cut(s) 1422
BstAPI GCANNNNNTGC 1 cut(s) 995
BstC8I GCNNGC 6 cut(s) 297, 504, 803, 987, 996, 1131
BstDEI CTNAG 2 cut(s) 254, 1025
BstDSI CCRYGG 2 cut(s) 1376, 1425
BstF5I GGATG 6 cut(s) 49, 222, 496, 946, 1087, 1276
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 3 cut(s) 520, 1298, 1400
BstKTI GATC 6 cut(s) 20, 34, 367, 603, 694, 1315
BstMAI GTCTC 2 cut(s) 42, 172
BstMBI GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
BstMWI GCNNNNNNNGC 6 cut(s) 305, 524, 889, 991, 995, 1302
BstNI CCWGG 3 cut(s) 159, 662, 807
BstNSI RCATGY 2 cut(s) 878, 1129
BstSCI CCNGG 3 cut(s) 157, 660, 805
BstSFI CTRYAG 1 cut(s) 987
BstV1I GCAGC 5 cut(s) 409, 486, 610, 785, 985
BstV2I GAAGAC 2 cut(s) 916, 942
BstX2I RGATCY 2 cut(s) 17, 364
BstYI RGATCY 2 cut(s) 17, 364
Bsu15I ATCGAT 1 cut(s) 603
BsuRI GGCC 3 cut(s) 805, 1351, 1386
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 2 cut(s) 1376, 1425
BtsCI GGATG 6 cut(s) 49, 222, 496, 946, 1087, 1276
BtsIMutI CAGTG 2 cut(s) 55, 745
BveI ACCTGC 1 cut(s) 1064
Cac8I GCNNGC 6 cut(s) 297, 504, 803, 987, 996, 1131
CfoI GCGC 3 cut(s) 520, 1298, 1400
Cfr13I GGNCC 4 cut(s) 155, 788, 1349, 1385
ClaI ATCGAT 1 cut(s) 603
CseI GACGC 1 cut(s) 1430
Csp6I GTAC 2 cut(s) 74, 579
CviAII CATG 5 cut(s) 875, 972, 1126, 1377, 1426
CviQI GTAC 2 cut(s) 74, 579
DdeI CTNAG 2 cut(s) 254, 1025
DpnI GATC 6 cut(s) 19, 33, 366, 602, 693, 1314
DpnII GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Eam1104I CTCTTC 2 cut(s) 711, 1203
EarI CTCTTC 2 cut(s) 711, 1203
Eco130I CCWWGG 2 cut(s) 1376, 1425
Eco47I GGWCC 2 cut(s) 155, 788
Eco57I CTGAAG 1 cut(s) 1065
Eco88I CYCGRG 1 cut(s) 513
EcoO109I RGGNCCY 2 cut(s) 155, 1385
EcoRI GAATTC 3 cut(s) 134, 354, 1162
EcoRII CCWGG 3 cut(s) 157, 660, 805
EcoT14I CCWWGG 2 cut(s) 1376, 1425
EcoT22I ATGCAT 1 cut(s) 650
ErhI CCWWGG 2 cut(s) 1376, 1425
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 5 cut(s) 878, 975, 1129, 1380, 1429
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 4 cut(s) 188, 774, 848, 1050
FatI CATG 5 cut(s) 874, 971, 1125, 1376, 1425
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 6 cut(s) 423, 500, 624, 799, 963, 999
FokI GGATG 6 cut(s) 56, 209, 503, 953, 1074, 1283
Fsp4HI GCNGC 6 cut(s) 423, 500, 624, 799, 963, 999
FspBI CTAG 1 cut(s) 825
FspI TGCGCA 2 cut(s) 1297, 1399
GlaI GCGC 3 cut(s) 519, 1297, 1399
GluI GCNGC 6 cut(s) 423, 500, 624, 799, 963, 999
GsaI CCCAGC 2 cut(s) 906, 1367
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 3 cut(s) 805, 1351, 1386
HgaI GACGC 1 cut(s) 1430
HhaI GCGC 3 cut(s) 520, 1298, 1400
Hin1I GRCGYC 1 cut(s) 1422
Hin1II CATG 5 cut(s) 878, 975, 1129, 1380, 1429
Hin6I GCGC 3 cut(s) 518, 1296, 1398
HinP1I GCGC 3 cut(s) 518, 1296, 1398
HincII GTYRAC 2 cut(s) 372, 1069
HindII GTYRAC 2 cut(s) 372, 1069
HindIII AAGCTT 2 cut(s) 890, 1184
HinfI GANTC 4 cut(s) 235, 480, 638, 1259
HphI GGTGA 2 cut(s) 272, 848
Hpy166II GTNNAC 4 cut(s) 372, 1069, 1171, 1342
Hpy188III TCNNGA 4 cut(s) 41, 248, 642, 682
Hpy8I GTNNAC 4 cut(s) 372, 1069, 1171, 1342
HpyCH4III ACNGT 4 cut(s) 586, 616, 688, 1456
HpyCH4V TGCA 6 cut(s) 64, 527, 626, 648, 989, 1274
HpyF10VI GCNNNNNNNGC 6 cut(s) 305, 524, 889, 991, 995, 1302
HpyF3I CTNAG 2 cut(s) 254, 1025
Hsp92I GRCGYC 1 cut(s) 1422
Hsp92II CATG 5 cut(s) 878, 975, 1129, 1380, 1429
HspAI GCGC 3 cut(s) 518, 1296, 1398
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
LmnI GCTCC 2 cut(s) 250, 724
Lsp1109I GCAGC 5 cut(s) 409, 486, 610, 785, 985
LweI GCATC 8 cut(s) 34, 635, 635, 931, 942, 1261, 1283, 1376
MaeI CTAG 1 cut(s) 825
MaeIII GTNAC 4 cut(s) 586, 616, 730, 836
MalI GATC 6 cut(s) 19, 33, 366, 602, 693, 1314
MboI GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
MfeI CAATTG 2 cut(s) 149, 831
MflI RGATCY 2 cut(s) 17, 364
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 2 cut(s) 590, 1127
Mph1103I ATGCAT 1 cut(s) 650
MroXI GAANNNNTTC 1 cut(s) 358
MseI TTAA 2 cut(s) 761, 1014
MslI CAYNNNNRTG 2 cut(s) 182, 845
MspA1I CMGCKG 2 cut(s) 422, 798
MspR9I CCNGG 3 cut(s) 159, 662, 807
MunI CAATTG 2 cut(s) 149, 831
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 3 cut(s) 159, 662, 807
MwoI GCNNNNNNNGC 6 cut(s) 305, 524, 889, 991, 995, 1302
NcoI CCATGG 2 cut(s) 1376, 1425
NdeII GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
NlaIII CATG 5 cut(s) 878, 975, 1129, 1380, 1429
NlaIV GGNNCC 4 cut(s) 156, 366, 790, 1387
NmuCI GTSAC 3 cut(s) 586, 616, 836
NsbI TGCGCA 2 cut(s) 1297, 1399
NsiI ATGCAT 1 cut(s) 650
NspI RCATGY 2 cut(s) 878, 1129
OliI CACNNNNGTG 1 cut(s) 845
PasI CCCWGGG 1 cut(s) 661
PciI ACATGT 1 cut(s) 874
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 480, 638, 1259
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 6 cut(s) 424, 501, 625, 800, 964, 1000
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 874
PsiI TTATAA 2 cut(s) 335, 917
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 3 cut(s) 157, 660, 805
PspFI CCCAGC 2 cut(s) 902, 1363
PspGI CCWGG 3 cut(s) 157, 660, 805
PspN4I GGNNCC 4 cut(s) 156, 366, 790, 1387
PspPI GGNCC 4 cut(s) 155, 788, 1349, 1385
PspPPI RGGWCCY 1 cut(s) 155
PstI CTGCAG 1 cut(s) 991
PsuI RGATCY 2 cut(s) 17, 364
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 2 cut(s) 75, 580
RsaNI GTAC 2 cut(s) 74, 579
RseI CAYNNNNRTG 2 cut(s) 182, 845
SaqAI TTAA 2 cut(s) 761, 1014
SatI GCNGC 6 cut(s) 423, 500, 624, 799, 963, 999
Sau3AI GATC 6 cut(s) 17, 31, 364, 600, 691, 1312
Sau96I GGNCC 4 cut(s) 155, 788, 1349, 1385
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 3 cut(s) 159, 662, 807
SfaNI GCATC 8 cut(s) 34, 635, 635, 931, 942, 1261, 1283, 1376
SfcI CTRYAG 1 cut(s) 987
SinI GGWCC 2 cut(s) 155, 788
SmiMI CAYNNNNRTG 2 cut(s) 182, 845
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
SsiI CCGC 3 cut(s) 473, 796, 962
SspMI CTAG 1 cut(s) 825
StyD4I CCNGG 3 cut(s) 157, 660, 805
StyI CCWWGG 2 cut(s) 1376, 1425
TaaI ACNGT 4 cut(s) 586, 616, 688, 1456
TaqI TCGA 5 cut(s) 30, 603, 643, 976, 1239
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 3 cut(s) 480, 638, 1259
Tru1I TTAA 2 cut(s) 761, 1014
Tru9I TTAA 2 cut(s) 761, 1014
TscAI CASTG 2 cut(s) 55, 745
TseFI GTSAC 3 cut(s) 586, 616, 836
TseI GCWGC 5 cut(s) 422, 499, 623, 798, 998
Tsp45I GTSAC 3 cut(s) 586, 616, 836
TspDTI ATGAA 7 cut(s) 147, 224, 347, 507, 726, 1127, 1257
TspGWI ACGGA 2 cut(s) 24, 357
TspRI CASTG 2 cut(s) 55, 745
VpaK11BI GGWCC 2 cut(s) 155, 788
XapI RAATTY 7 cut(s) 79, 134, 354, 442, 551, 1162, 1276
XceI RCATGY 2 cut(s) 878, 1129
XcmI CCANNNNNNNNNTGG 1 cut(s) 1453
XmnI GAANNNNTTC 1 cut(s) 358
XspI CTAG 1 cut(s) 825
Zsp2I ATGCAT 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.