MD12G1209500.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005594
Physical Location & Seq
Forward (+)
28850878 .. 28854361
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1209500.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGGCTTCAATGTCGGATCCTCCCTGCTCGATCCGTCTCTGACTGTCAGTGTTTTTGCTCTGCATTCGAAGTTGATGGACGTACGAGGGTTTTGGCACTTGGGTCTTGGAAATGTCCCAACGGACACTAAAATGAGCCAGATTGAAGTATCGGCGATGAAATCTCCTCTCACTCCACTTGCGACTCTGATTGGTCGTGAGCTTAGGGGTGAGAAAGTTGAGAAACCTTATTTGAAGTATGGGCAGGCTGGTTTGGCGAAGAAAGGAGAAGATTACTTTCTTATAAAAACCGATTGCCAAAGAATTCATTCGGATCCGTCAACATCATTTTCTGTCTTTGCGATTTTTGATGGGCATAATGGTATATCAGCTGCTATTTTTACCAAGGAGAATTTATTGGCTAATGTTTTGAGTGCCATTCCTCAAGGAACCAGTAGGGATGAATGGCTGCAAGCCCTTCCTCGGGCGCTGGTTGCAGGATTTGTCAAAACTGATATAGAATTTCAACAGAAAGGGGAAACTTCTGGTACAACTGTGACTTTTGTTGTGATTGATGATTGGACTGTGACTGTTGCATCTGTTGGGGATTCACGATGTATATTAGACACCCAGGGAGGTGTTGTTTCACTCTTAACTGTTGATCACAGGCTGGAAGAAAATGAAGAAGAGAGGGAGCGTGTTACTGCCAGTGGAGGTGAAGTAGGAAGGCTCAATATTTTTGGGGGCAATGAGGTTGGTCCCCTTCGCTGTTGGCCTGGTGGATTATGCCTTTCTAGGTCAATTGGCGACACAGATGTGGGAGAATACATTGTCCCAATACCCCATGTCAAGCAAGTGAAGCTTTCAAATGCTGGGGGAAGACTTATAATAGCTTCTGATGGCATCTGGGATGCTTTGTCATCTGATATGGCTGCCAAGTCATGTCGAGGATTGCCTGCAGAGCTTGCTGCAAAGCTGGTCGTTAAGGAGGCTCTGAGGTCAAGGGGACTGAAAGATGATACAACCTGCGTAGTTGTTGACATAATCCCTTCAGGCCATACTGTCTTGCCTCCACCACCAATTAAGAAACCAAACATGCTTGCTTCGCTTATCTTTGGGAAGAAAAATCAGAACTCCATGAACAAGACAAATAAACTTTCTGCTATCGGAGTTGTGGAGGAGTTGTTTGAAGAAGGTTCAGCAATGCTCGATGAAAGGTTGGGTAAGGATTCTCCTCTGAATGCTAATTCGGGGATTTTCCGTTGCGCAGTCTGCCAAGTGGATCAGCCCCAAGTTGAAGGTTTATCAGTAAACTCTGCGCCTTTCTTCTCCCCGGCATCAAAGCCATGGGAAGGCCCCTTCCTCTGCGCAAAGTGTCAGAACAAGAAAGACGCCATGGAAGGAAAAAGACTGACAAGACCCACAGTGGTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

472

Amino Acids

50.75

Weight (kDa)

6.23

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 105 - 324 1.6e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 284, 866
Acc16I TGCGCA 2 cut(s) 1246, 1348
Acc36I ACCTGC 1 cut(s) 1013
AclWI GGATC 6 cut(s) 12, 25, 26, 308, 321, 1269
AcsI RAATTY 3 cut(s) 303, 391, 500
AcuI CTGAAG 1 cut(s) 1014
AcyI GRCGYC 1 cut(s) 1371
AfaI GTAC 2 cut(s) 84, 529
AfiI CCNNNNNNNGG 3 cut(s) 462, 463, 1331
AgsI TTSAA 7 cut(s) 10, 146, 235, 506, 846, 1169, 1277
AjnI CCWGG 2 cut(s) 609, 754
AleI CACNNNNGTG 1 cut(s) 794
AluBI AGCT 6 cut(s) 202, 371, 841, 872, 943, 955
AluI AGCT 6 cut(s) 202, 371, 841, 872, 943, 955
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 6 cut(s) 12, 25, 26, 308, 321, 1269
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 3 cut(s) 752, 1033, 1333
ApeKI GCWGC 4 cut(s) 371, 448, 911, 947
ApoI RAATTY 3 cut(s) 303, 391, 500
Asp700I GAANNNNTTC 1 cut(s) 307
AspLEI GCGC 4 cut(s) 469, 1247, 1300, 1349
AspS9I GGNCC 2 cut(s) 737, 1334
AsuC2I CCSGG 1 cut(s) 1313
AsuHPI GGTGA 2 cut(s) 221, 707
AsuII TTCGAA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 1 cut(s) 737
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BamHI GGATCC 2 cut(s) 17, 313
BbsI GAAGAC 1 cut(s) 865
BbvI GCAGC 4 cut(s) 358, 435, 898, 934
BccI CCATC 3 cut(s) 70, 344, 872
BciT130I CCWGG 2 cut(s) 611, 756
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 1 cut(s) 1313
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 1 cut(s) 774
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 1013
BisI GCNGC 4 cut(s) 372, 449, 912, 948
BlsI GCNGC 4 cut(s) 373, 450, 913, 949
Bme1390I CCNGG 3 cut(s) 611, 756, 1313
Bme18I GGWCC 1 cut(s) 737
BmeT110I CYCGRG 1 cut(s) 462
BmgT120I GGNCC 2 cut(s) 737, 1334
BmiI GGNNCC 5 cut(s) 19, 315, 430, 739, 1336
BmrFI CCNGG 3 cut(s) 611, 756, 1313
BmsI GCATC 4 cut(s) 584, 880, 891, 1325
BpiI GAAGAC 1 cut(s) 865
Bpu10I CCTNAGC 1 cut(s) 203
Bpu14I TTCGAA 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 408
BpuMI CCSGG 1 cut(s) 1313
BsaHI GRCGYC 1 cut(s) 1371
BsaJI CCNNGG 7 cut(s) 384, 461, 609, 610, 1311, 1325, 1374
BsaXI ACNNNNNCTCC 6 cut(s) 606, 636, 684, 714, 1148, 1178
Bsc4I CCNNNNNNNGG 3 cut(s) 462, 463, 1331
Bse1I ACTGG 2 cut(s) 432, 687
Bse3DI GCAATG 2 cut(s) 733, 1188
BseBI CCWGG 2 cut(s) 611, 756
BseDI CCNNGG 7 cut(s) 384, 461, 609, 610, 1311, 1325, 1374
BseGI GGATG 2 cut(s) 445, 895
BseLI CCNNNNNNNGG 3 cut(s) 462, 463, 1331
BseMI GCAATG 2 cut(s) 733, 1188
BseMII CTCAG 1 cut(s) 965
BseNI ACTGG 2 cut(s) 432, 687
BseRI GAGGAG 3 cut(s) 156, 1172, 1203
BseXI GCAGC 4 cut(s) 358, 435, 898, 934
BseYI CCCAGC 1 cut(s) 851
BshFI GGCC 3 cut(s) 754, 1035, 1335
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 1 cut(s) 1313
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 4 cut(s) 101, 723, 797, 999
BslI CCNNNNNNNGG 3 cut(s) 462, 463, 1331
BsmAI GTCTC 1 cut(s) 42
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 4 cut(s) 101, 723, 797, 999
BsmI GAATGC 2 cut(s) 64, 1225
BsnI GGCC 3 cut(s) 754, 1035, 1335
BsoBI CYCGRG 1 cut(s) 462
Bsp119I TTCGAA 1 cut(s) 68
Bsp143I GATC 5 cut(s) 17, 31, 313, 640, 1261
Bsp19I CCATGG 2 cut(s) 1325, 1374
BspANI GGCC 3 cut(s) 754, 1035, 1335
BspCNI CTCAG 1 cut(s) 966
BspLI GGNNCC 5 cut(s) 19, 315, 430, 739, 1336
BspMAI CTGCAG 1 cut(s) 940
BspMI ACCTGC 1 cut(s) 1013
BspPI GGATC 6 cut(s) 12, 25, 26, 308, 321, 1269
BspT104I TTCGAA 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 733, 1188
BsrI ACTGG 2 cut(s) 432, 687
BssECI CCNNGG 7 cut(s) 384, 461, 609, 610, 1311, 1325, 1374
BssMI GATC 5 cut(s) 17, 31, 313, 640, 1261
BssNI GRCGYC 1 cut(s) 1371
BssT1I CCWWGG 3 cut(s) 384, 1325, 1374
Bst2UI CCWGG 2 cut(s) 611, 756
Bst4CI ACNGT 7 cut(s) 46, 535, 565, 571, 637, 1042, 1405
Bst6I CTCTTC 1 cut(s) 660
BstACI GRCGYC 1 cut(s) 1371
BstAPI GCANNNNNTGC 1 cut(s) 944
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 5 cut(s) 246, 453, 936, 945, 1080
BstDEI CTNAG 2 cut(s) 203, 974
BstDSI CCRYGG 2 cut(s) 1325, 1374
BstF5I GGATG 2 cut(s) 445, 895
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 4 cut(s) 469, 1247, 1300, 1349
BstKTI GATC 5 cut(s) 20, 34, 316, 643, 1264
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 5 cut(s) 17, 31, 313, 640, 1261
BstMWI GCNNNNNNNGC 7 cut(s) 254, 473, 838, 940, 944, 1084, 1251
BstNI CCWGG 2 cut(s) 611, 756
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 3 cut(s) 609, 754, 1311
BstSFI CTRYAG 1 cut(s) 936
BstV1I GCAGC 4 cut(s) 358, 435, 898, 934
BstV2I GAAGAC 1 cut(s) 865
BstX2I RGATCY 2 cut(s) 17, 313
BstYI RGATCY 2 cut(s) 17, 313
BsuRI GGCC 3 cut(s) 754, 1035, 1335
BtgI CCRYGG 2 cut(s) 1325, 1374
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 2 cut(s) 445, 895
BtsIMutI CAGTG 3 cut(s) 55, 694, 1410
BveI ACCTGC 1 cut(s) 1013
Cac8I GCNNGC 5 cut(s) 246, 453, 936, 945, 1080
CfoI GCGC 4 cut(s) 469, 1247, 1300, 1349
Cfr13I GGNCC 2 cut(s) 737, 1334
CseI GACGC 1 cut(s) 1379
Csp6I GTAC 2 cut(s) 83, 528
CviAII CATG 6 cut(s) 824, 921, 1075, 1117, 1326, 1375
CviQI GTAC 2 cut(s) 83, 528
DdeI CTNAG 2 cut(s) 203, 974
DpnI GATC 5 cut(s) 19, 33, 315, 642, 1263
DpnII GATC 5 cut(s) 17, 31, 313, 640, 1261
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Eco130I CCWWGG 3 cut(s) 384, 1325, 1374
Eco47I GGWCC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 1014
Eco88I CYCGRG 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 1334
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 609, 754
EcoT14I CCWWGG 3 cut(s) 384, 1325, 1374
ErhI CCWWGG 3 cut(s) 384, 1325, 1374
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 6 cut(s) 827, 924, 1078, 1120, 1329, 1378
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 4 cut(s) 101, 723, 797, 999
FatI CATG 6 cut(s) 823, 920, 1074, 1116, 1325, 1374
FbaI TGATCA 1 cut(s) 640
Fnu4HI GCNGC 4 cut(s) 372, 449, 912, 948
FokI GGATG 2 cut(s) 452, 902
Fsp4HI GCNGC 4 cut(s) 372, 449, 912, 948
FspBI CTAG 1 cut(s) 774
FspI TGCGCA 2 cut(s) 1246, 1348
GlaI GCGC 4 cut(s) 468, 1246, 1299, 1348
GluI GCNGC 4 cut(s) 372, 449, 912, 948
GsaI CCCAGC 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 3 cut(s) 754, 1035, 1335
HapII CCGG 1 cut(s) 1313
HgaI GACGC 1 cut(s) 1379
HhaI GCGC 4 cut(s) 469, 1247, 1300, 1349
Hin1I GRCGYC 1 cut(s) 1371
Hin1II CATG 6 cut(s) 827, 924, 1078, 1120, 1329, 1378
Hin6I GCGC 4 cut(s) 467, 1245, 1298, 1347
HinP1I GCGC 4 cut(s) 467, 1245, 1298, 1347
HincII GTYRAC 2 cut(s) 321, 1018
HindII GTYRAC 2 cut(s) 321, 1018
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 3 cut(s) 184, 587, 1208
HpaII CCGG 1 cut(s) 1313
HphI GGTGA 2 cut(s) 221, 707
Hpy166II GTNNAC 3 cut(s) 321, 1018, 1291
Hpy188III TCNNGA 2 cut(s) 197, 591
Hpy8I GTNNAC 3 cut(s) 321, 1018, 1291
HpyAV CCTTC 9 cut(s) 467, 699, 752, 1038, 1166, 1271, 1325, 1348, 1373
HpyCH4III ACNGT 7 cut(s) 46, 535, 565, 571, 637, 1042, 1405
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 6 cut(s) 64, 451, 476, 575, 938, 950
HpyF10VI GCNNNNNNNGC 7 cut(s) 254, 473, 838, 940, 944, 1084, 1251
HpyF3I CTNAG 2 cut(s) 203, 974
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 1371
Hsp92II CATG 6 cut(s) 827, 924, 1078, 1120, 1329, 1378
HspAI GCGC 4 cut(s) 467, 1245, 1298, 1347
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 5 cut(s) 17, 31, 313, 640, 1261
LmnI GCTCC 1 cut(s) 673
Lsp1109I GCAGC 4 cut(s) 358, 435, 898, 934
LweI GCATC 4 cut(s) 584, 880, 891, 1325
MaeI CTAG 1 cut(s) 774
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 3 cut(s) 535, 565, 679
MalI GATC 5 cut(s) 19, 33, 315, 642, 1263
MboI GATC 5 cut(s) 17, 31, 313, 640, 1261
MboII GAAGA 9 cut(s) 271, 281, 665, 674, 677, 870, 1111, 1181, 1297
MfeI CAATTG 1 cut(s) 780
MflI RGATCY 2 cut(s) 17, 313
MluCI AATT 6 cut(s) 303, 391, 500, 780, 1059, 1225
MlyI GAGTC 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 307
MseI TTAA 3 cut(s) 632, 963, 1062
MslI CAYNNNNRTG 2 cut(s) 131, 794
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 1313
MspR9I CCNGG 3 cut(s) 611, 756, 1313
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 2 cut(s) 64, 1225
MvaI CCWGG 2 cut(s) 611, 756
MwoI GCNNNNNNNGC 7 cut(s) 254, 473, 838, 940, 944, 1084, 1251
NciI CCSGG 1 cut(s) 1313
NcoI CCATGG 2 cut(s) 1325, 1374
NdeII GATC 5 cut(s) 17, 31, 313, 640, 1261
NlaIII CATG 6 cut(s) 827, 924, 1078, 1120, 1329, 1378
NlaIV GGNNCC 5 cut(s) 19, 315, 430, 739, 1336
NmuCI GTSAC 2 cut(s) 535, 565
NsbI TGCGCA 2 cut(s) 1246, 1348
NspI RCATGY 1 cut(s) 1078
NspV TTCGAA 1 cut(s) 68
OliI CACNNNNGTG 1 cut(s) 794
PasI CCCWGGG 1 cut(s) 610
PctI GAATGC 2 cut(s) 64, 1225
PdmI GAANNNNTTC 1 cut(s) 307
PfeI GAWTC 2 cut(s) 587, 1208
Pfl23II CGTACG 1 cut(s) 82
PkrI GCNGC 4 cut(s) 373, 450, 913, 949
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PsiI TTATAA 2 cut(s) 284, 866
Psp6I CCWGG 2 cut(s) 609, 754
PspFI CCCAGC 1 cut(s) 851
PspGI CCWGG 2 cut(s) 609, 754
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 5 cut(s) 19, 315, 430, 739, 1336
PspPI GGNCC 2 cut(s) 737, 1334
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 2 cut(s) 17, 313
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 2 cut(s) 84, 529
RsaNI GTAC 2 cut(s) 83, 528
RseI CAYNNNNRTG 2 cut(s) 131, 794
SaqAI TTAA 3 cut(s) 632, 963, 1062
SatI GCNGC 4 cut(s) 372, 449, 912, 948
Sau3AI GATC 5 cut(s) 17, 31, 313, 640, 1261
Sau96I GGNCC 2 cut(s) 737, 1334
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 3 cut(s) 611, 756, 1313
SfaNI GCATC 4 cut(s) 584, 880, 891, 1325
SfcI CTRYAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 68
SinI GGWCC 1 cut(s) 737
SmiMI CAYNNNNRTG 2 cut(s) 131, 794
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 6 cut(s) 303, 391, 500, 780, 1059, 1225
SspI AATATT 1 cut(s) 715
SspMI CTAG 1 cut(s) 774
StyD4I CCNGG 3 cut(s) 609, 754, 1311
StyI CCWWGG 3 cut(s) 384, 1325, 1374
TaaI ACNGT 7 cut(s) 46, 535, 565, 571, 637, 1042, 1405
TaiI ACGT 1 cut(s) 84
TaqI TCGA 4 cut(s) 30, 68, 925, 1188
TasI AATT 6 cut(s) 303, 391, 500, 780, 1059, 1225
TfiI GAWTC 2 cut(s) 587, 1208
Tru1I TTAA 3 cut(s) 632, 963, 1062
Tru9I TTAA 3 cut(s) 632, 963, 1062
TscAI CASTG 3 cut(s) 55, 694, 1410
TseFI GTSAC 2 cut(s) 535, 565
TseI GCWGC 4 cut(s) 371, 448, 911, 947
Tsp45I GTSAC 2 cut(s) 535, 565
TspDTI ATGAA 6 cut(s) 173, 296, 456, 675, 1133, 1206
TspGWI ACGGA 4 cut(s) 24, 137, 306, 1229
TspRI CASTG 3 cut(s) 55, 694, 1410
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 3 cut(s) 303, 391, 500
XceI RCATGY 1 cut(s) 1078
XmnI GAANNNNTTC 1 cut(s) 307
XspI CTAG 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.