RLG00000025289

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005594
Physical Location & Seq
Forward (+)
43632321 .. 43635701
3381 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025289

Sequence Viewer

Length: 1350 bp
ATGTCTTTTGATACTCTTGGTGTTCTAAGGTTTTGGCACTTGTCTCTTGAAAATGTCACAAGGACTAAAATGAGTCAGACTGAAGTAGCGAGGATGAAATCTCCTCTTGTTCCACTTGCAACATTGATTGGGCGTGAGATTAGGGGCGAGAAGGTTGAGGAACCCTATTTGAAGTATGGGCAGGCTGCTTTGGCAAAGAAAGGAGAGGATTATTTTCTGATAAAACCAGATTGCCAAAGAATTCATGCTAATCCTTCAACCTCATTTTCTGTTTTTGCGATATTTGATGGGCATAATGGTAACTCAGCTGCTATTTATGCAAAGGAGACTTTATTGGATAATGTTTTGAGTGCAATTCCTCAAGGTAGCAGTAGGGACGAATGGCTTCAAGCCCTGCCTCGGGCACTGGTTGCCGGTTTTGTGAAAACTGATATAGAATTTCAGCAGAAAGGGGAAACATCTGGTACAACTGTTACATTCGTTGTAATTGATGGTTGGACTGTGACTGTGGCATCTGTTGGGGATTCTCGATGCATATTAGACACCCAGGGAGGTGTGGTTTCACTATTGACTGTTGATCACAGGCTGGAAGAAAATGAAGAGGAGAGGGAACGTGTGACAGCTAGTGGGGGTGAAGTAGGAAGGCTTAATGTCTTTGGGGGCAGTGAGGTCGGTCCCCTACGCTGCTGGCCTGGTGGGTTATGCCTTTCTAGGTCAATTGGTGACACAGACGTGGGTGAATTTATTGTCCCAGTACCACATGTCAAGCAAGTAAAGCTTTCAAATGCCGGGGGAAGACTTATAATAGCTTCTGATGGCATCTGGGATGCTCTGTCTTCTGATATGGCTGCCAAGTCTTGTCGGGGATTGCCTGCAGAGCTTGCTGCAAAGTTGGTCGTTAAGGAGGCTCTGAGGTCAAGGGGTCTGAAAGATGATACAACCTGCGTGGTTGTTGACATAATCCCATCAGACCGTCCTGTCTTGCCTCCAACTCCGAGGAAGAAACAAAGCATGCTCACTTCACTTATTTTTGGGAAGAAAAGTCAGAATGGGAGCAAAACAAATAAGCTTTCAGCGGTGGGAGTTGTGGAGGAACTGTTTGAAGAAGGTTCAGCAATGCTCGCTGAAAGGTTGGGTAAGGATATTCCTTTGGATGCAAATTCTGGTATTTTCCGGTGTGCAGTCTGCCAAGTTGATCAGCCCCAATCAGAAGGTTTATCAGTAAATTCTGGACCTTTTTTCTCACCATCATCAAAGCCATGGGAAGGCCCCTTCCTCTGCACAACCTGTCTGAAAAAGAAAGACGCCATGGAAGGAAAAAGAATGAGCACACCCACAGTGGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

450

Amino Acids

48.5

Weight (kDa)

6.3

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 84 - 303 3e-34 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 803
AasI GACNNNNNNGTC 1 cut(s) 977
Acc36I ACCTGC 1 cut(s) 950
AciI CCGC 1 cut(s) 1076
AcsI RAATTY 5 cut(s) 240, 437, 740, 1159, 1225
AcuI CTGAAG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 1305
AfaI GTAC 2 cut(s) 466, 756
AfiI CCNNNNNNNGG 3 cut(s) 399, 400, 1265
AflIII ACRYGT 2 cut(s) 613, 760
AgsI TTSAA 6 cut(s) 50, 172, 258, 389, 783, 1103
AjiI CACGTC 1 cut(s) 733
AjnI CCWGG 2 cut(s) 546, 691
AleI CACNNNNGTG 1 cut(s) 731
AluBI AGCT 6 cut(s) 308, 623, 778, 809, 880, 1069
AluI AGCT 6 cut(s) 308, 623, 778, 809, 880, 1069
Alw21I GWGCWC 1 cut(s) 1331
Alw26I GTCTC 2 cut(s) 48, 320
Ama87I CYCGRG 1 cut(s) 399
AoxI GGCC 2 cut(s) 689, 1267
ApeKI GCWGC 5 cut(s) 185, 308, 684, 848, 884
ApoI RAATTY 5 cut(s) 240, 437, 740, 1159, 1225
Asp700I GAANNNNTTC 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 674, 1232, 1268
AsuC2I CCSGG 1 cut(s) 790
AsuHPI GGTGA 4 cut(s) 644, 734, 749, 1236
AvaI CYCGRG 1 cut(s) 399
AvaII GGWCC 2 cut(s) 674, 1232
BaeGI GKGCMC 1 cut(s) 406
BaeI ACNNNNGTAYC 2 cut(s) 456, 489
BbsI GAAGAC 2 cut(s) 802, 828
Bbv12I GWGCWC 1 cut(s) 1331
BbvI GCAGC 5 cut(s) 172, 295, 671, 835, 871
BccI CCATC 5 cut(s) 281, 485, 809, 973, 1255
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 2 cut(s) 548, 693
BclI TGATCA 2 cut(s) 577, 1195
BcnI CCSGG 1 cut(s) 790
BcoDI GTCTC 2 cut(s) 48, 320
BfaI CTAG 2 cut(s) 624, 711
BfmI CTRYAG 1 cut(s) 873
BfuAI ACCTGC 1 cut(s) 950
BisI GCNGC 5 cut(s) 186, 309, 685, 849, 885
BlsI GCNGC 5 cut(s) 187, 310, 686, 850, 886
Bme1390I CCNGG 3 cut(s) 548, 693, 790
Bme18I GGWCC 2 cut(s) 674, 1232
BmeT110I CYCGRG 1 cut(s) 399
BmgBI CACGTC 1 cut(s) 733
BmgT120I GGNCC 3 cut(s) 674, 1232, 1268
BmiI GGNNCC 3 cut(s) 162, 676, 1270
BmrFI CCNGG 3 cut(s) 548, 693, 790
BmrI ACTGGG 1 cut(s) 746
BmsI GCATC 5 cut(s) 521, 521, 817, 828, 1144
BmuI ACTGGG 1 cut(s) 746
BpiI GAAGAC 2 cut(s) 802, 828
BpuEI CTTGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 790
BsaHI GRCGYC 1 cut(s) 1305
BsaJI CCNNGG 7 cut(s) 398, 546, 547, 789, 995, 1259, 1308
BsaWI WCCGGW 1 cut(s) 1173
BsaXI ACNNNNNCTCC 4 cut(s) 543, 573, 1082, 1112
Bsc4I CCNNNNNNNGG 3 cut(s) 399, 400, 1265
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 2 cut(s) 411, 752
Bse3DI GCAATG 1 cut(s) 1122
BseBI CCWGG 2 cut(s) 548, 693
BseDI CCNNGG 7 cut(s) 398, 546, 547, 789, 995, 1259, 1308
BseGI GGATG 3 cut(s) 99, 832, 1159
BseLI CCNNNNNNNGG 3 cut(s) 399, 400, 1265
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 2 cut(s) 318, 902
BseNI ACTGG 2 cut(s) 411, 752
BseRI GAGGAG 2 cut(s) 93, 617
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 5 cut(s) 172, 295, 671, 835, 871
BsgI GTGCAG 2 cut(s) 1200, 1264
BshFI GGCC 2 cut(s) 691, 1269
BsiHKAI GWGCWC 1 cut(s) 1331
BsiHKCI CYCGRG 1 cut(s) 399
BsiSI CCGG 3 cut(s) 414, 789, 1174
BslFI GGGAC 3 cut(s) 389, 660, 734
BslI CCNNNNNNNGG 3 cut(s) 399, 400, 1265
BsmAI GTCTC 2 cut(s) 48, 320
BsmFI GGGAC 3 cut(s) 389, 660, 734
BsnI GGCC 2 cut(s) 691, 1269
BsoBI CYCGRG 1 cut(s) 399
Bsp1286I GDGCHC 2 cut(s) 406, 1331
Bsp143I GATC 2 cut(s) 577, 1195
Bsp19I CCATGG 2 cut(s) 1259, 1308
BspACI CCGC 1 cut(s) 1076
BspANI GGCC 2 cut(s) 691, 1269
BspCNI CTCAG 2 cut(s) 317, 903
BspLI GGNNCC 3 cut(s) 162, 676, 1270
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 1 cut(s) 950
BsrDI GCAATG 1 cut(s) 1122
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 2 cut(s) 411, 752
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 7 cut(s) 398, 546, 547, 789, 995, 1259, 1308
BssMI GATC 2 cut(s) 577, 1195
BssNI GRCGYC 1 cut(s) 1305
BssT1I CCWWGG 2 cut(s) 1259, 1308
Bst2UI CCWGG 2 cut(s) 548, 693
Bst4CI ACNGT 7 cut(s) 472, 502, 508, 574, 974, 1098, 1339
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 1305
BstAPI GCANNNNNTGC 2 cut(s) 410, 881
BstC8I GCNNGC 6 cut(s) 183, 689, 873, 882, 1013, 1122
BstDEI CTNAG 3 cut(s) 26, 304, 911
BstDSI CCRYGG 2 cut(s) 1259, 1308
BstF5I GGATG 3 cut(s) 99, 832, 1159
BstKTI GATC 2 cut(s) 580, 1198
BstMAI GTCTC 2 cut(s) 48, 320
BstMBI GATC 2 cut(s) 577, 1195
BstMWI GCNNNNNNNGC 7 cut(s) 191, 317, 410, 775, 877, 881, 1121
BstNI CCWGG 2 cut(s) 548, 693
BstNSI RCATGY 2 cut(s) 764, 1015
BstSCI CCNGG 3 cut(s) 546, 691, 788
BstSFI CTRYAG 1 cut(s) 873
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 5 cut(s) 172, 295, 671, 835, 871
BstV2I GAAGAC 2 cut(s) 802, 828
BsuRI GGCC 2 cut(s) 691, 1269
BtgI CCRYGG 2 cut(s) 1259, 1308
BtrI CACGTC 1 cut(s) 733
BtsCI GGATG 3 cut(s) 99, 832, 1159
BtsI GCAGTG 1 cut(s) 670
BtsIMutI CAGTG 3 cut(s) 404, 670, 1344
BveI ACCTGC 1 cut(s) 950
Cac8I GCNNGC 6 cut(s) 183, 689, 873, 882, 1013, 1122
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 3 cut(s) 674, 1232, 1268
CseI GACGC 1 cut(s) 1313
Csp6I GTAC 2 cut(s) 465, 755
CspCI CAANNNNNGTGG 2 cut(s) 927, 962
CviAII CATG 5 cut(s) 245, 761, 1012, 1260, 1309
CviQI GTAC 2 cut(s) 465, 755
DdeI CTNAG 3 cut(s) 26, 304, 911
DpnI GATC 2 cut(s) 579, 1197
DpnII GATC 2 cut(s) 577, 1195
DrdI GACNNNNNNGTC 1 cut(s) 977
DseDI GACNNNNNNGTC 1 cut(s) 977
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 1259, 1308
Eco47I GGWCC 2 cut(s) 674, 1232
Eco57I CTGAAG 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRI GAATTC 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 546, 691
EcoT14I CCWWGG 2 cut(s) 1259, 1308
EcoT22I ATGCAT 1 cut(s) 536
ErhI CCWWGG 2 cut(s) 1259, 1308
FaeI CATG 5 cut(s) 248, 764, 1015, 1263, 1312
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 3 cut(s) 389, 660, 734
FatI CATG 5 cut(s) 244, 760, 1011, 1259, 1308
FbaI TGATCA 2 cut(s) 577, 1195
Fnu4HI GCNGC 5 cut(s) 186, 309, 685, 849, 885
FokI GGATG 3 cut(s) 106, 839, 1166
Fsp4HI GCNGC 5 cut(s) 186, 309, 685, 849, 885
FspBI CTAG 2 cut(s) 624, 711
GluI GCNGC 5 cut(s) 186, 309, 685, 849, 885
HaeIII GGCC 2 cut(s) 691, 1269
HapII CCGG 3 cut(s) 414, 789, 1174
HgaI GACGC 1 cut(s) 1313
Hin1I GRCGYC 1 cut(s) 1305
Hin1II CATG 5 cut(s) 248, 764, 1015, 1263, 1312
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HindIII AAGCTT 2 cut(s) 776, 1067
HinfI GANTC 2 cut(s) 73, 524
HpaII CCGG 3 cut(s) 414, 789, 1174
HphI GGTGA 4 cut(s) 644, 734, 749, 1236
Hpy166II GTNNAC 1 cut(s) 955
Hpy188III TCNNGA 3 cut(s) 47, 528, 1230
Hpy8I GTNNAC 1 cut(s) 955
HpyAV CCTTC 8 cut(s) 145, 264, 636, 1100, 1205, 1259, 1282, 1307
HpyCH4III ACNGT 7 cut(s) 472, 502, 508, 574, 974, 1098, 1339
HpyCH4IV ACGT 2 cut(s) 613, 732
HpyCH4V TGCA 9 cut(s) 119, 320, 353, 534, 875, 887, 1157, 1181, 1281
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 317, 410, 775, 877, 881, 1121
HpyF3I CTNAG 3 cut(s) 26, 304, 911
HpySE526I ACGT 2 cut(s) 613, 732
Hsp92I GRCGYC 1 cut(s) 1305
Hsp92II CATG 5 cut(s) 248, 764, 1015, 1263, 1312
Ksp22I TGATCA 2 cut(s) 577, 1195
Kzo9I GATC 2 cut(s) 577, 1195
LmnI GCTCC 1 cut(s) 1053
Lsp1109I GCAGC 5 cut(s) 172, 295, 671, 835, 871
LweI GCATC 5 cut(s) 521, 521, 817, 828, 1144
MaeI CTAG 2 cut(s) 624, 711
MaeII ACGT 2 cut(s) 613, 732
MaeIII GTNAC 6 cut(s) 55, 299, 472, 502, 616, 722
MalI GATC 2 cut(s) 579, 1197
MboI GATC 2 cut(s) 577, 1195
MboII GAAGA 7 cut(s) 602, 611, 807, 828, 1012, 1048, 1115
MfeI CAATTG 1 cut(s) 717
MhlI GDGCHC 2 cut(s) 406, 1331
MluCI AATT 8 cut(s) 240, 354, 437, 486, 717, 740, 1159, 1225
MlyI GAGTC 1 cut(s) 82
MmeI TCCRAC 2 cut(s) 476, 1013
Mph1103I ATGCAT 1 cut(s) 536
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 2 cut(s) 648, 900
MslI CAYNNNNRTG 1 cut(s) 731
MspA1I CMGCKG 2 cut(s) 308, 1076
MspI CCGG 3 cut(s) 414, 789, 1174
MspR9I CCNGG 3 cut(s) 548, 693, 790
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 2 cut(s) 548, 693
MwoI GCNNNNNNNGC 7 cut(s) 191, 317, 410, 775, 877, 881, 1121
NciI CCSGG 1 cut(s) 790
NcoI CCATGG 2 cut(s) 1259, 1308
NdeII GATC 2 cut(s) 577, 1195
NlaIII CATG 5 cut(s) 248, 764, 1015, 1263, 1312
NlaIV GGNNCC 3 cut(s) 162, 676, 1270
NmuCI GTSAC 4 cut(s) 55, 502, 616, 722
NsiI ATGCAT 1 cut(s) 536
NspI RCATGY 2 cut(s) 764, 1015
OliI CACNNNNGTG 1 cut(s) 731
PaeI GCATGC 1 cut(s) 1015
PasI CCCWGGG 1 cut(s) 547
PciI ACATGT 1 cut(s) 760
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 524
PkrI GCNGC 5 cut(s) 187, 310, 686, 850, 886
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 1 cut(s) 760
PsiI TTATAA 1 cut(s) 803
Psp6I CCWGG 2 cut(s) 546, 691
PspGI CCWGG 2 cut(s) 546, 691
PspN4I GGNNCC 3 cut(s) 162, 676, 1270
PspPI GGNCC 3 cut(s) 674, 1232, 1268
PstI CTGCAG 1 cut(s) 877
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 2 cut(s) 466, 756
RsaNI GTAC 2 cut(s) 465, 755
RseI CAYNNNNRTG 1 cut(s) 731
SaqAI TTAA 2 cut(s) 648, 900
SatI GCNGC 5 cut(s) 186, 309, 685, 849, 885
Sau3AI GATC 2 cut(s) 577, 1195
Sau96I GGNCC 3 cut(s) 674, 1232, 1268
SchI GAGTC 1 cut(s) 82
ScrFI CCNGG 3 cut(s) 548, 693, 790
SduI GDGCHC 2 cut(s) 406, 1331
SfaNI GCATC 5 cut(s) 521, 521, 817, 828, 1144
SfcI CTRYAG 1 cut(s) 873
SinI GGWCC 2 cut(s) 674, 1232
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
SphI GCATGC 1 cut(s) 1015
Sse9I AATT 8 cut(s) 240, 354, 437, 486, 717, 740, 1159, 1225
SsiI CCGC 1 cut(s) 1076
SspMI CTAG 2 cut(s) 624, 711
StyD4I CCNGG 3 cut(s) 546, 691, 788
StyI CCWWGG 2 cut(s) 1259, 1308
TaaI ACNGT 7 cut(s) 472, 502, 508, 574, 974, 1098, 1339
TaiI ACGT 2 cut(s) 616, 735
TaqI TCGA 1 cut(s) 529
TaqII GACCGA 1 cut(s) 662
TasI AATT 8 cut(s) 240, 354, 437, 486, 717, 740, 1159, 1225
TfiI GAWTC 1 cut(s) 524
Tru1I TTAA 2 cut(s) 648, 900
Tru9I TTAA 2 cut(s) 648, 900
TscAI CASTG 3 cut(s) 411, 670, 1344
TseFI GTSAC 4 cut(s) 55, 502, 616, 722
TseI GCWGC 5 cut(s) 185, 308, 684, 848, 884
Tsp45I GTSAC 4 cut(s) 55, 502, 616, 722
TspDTI ATGAA 3 cut(s) 110, 233, 612
TspRI CASTG 3 cut(s) 411, 670, 1344
VpaK11BI GGWCC 2 cut(s) 674, 1232
XapI RAATTY 5 cut(s) 240, 437, 740, 1159, 1225
XceI RCATGY 2 cut(s) 764, 1015
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 2 cut(s) 624, 711
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.