FvH4_6g11750

Glycosyltransferase sugar-binding region containing DXD motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004935
Physical Location & Seq
Forward (+)
7020273 .. 7021529
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g11750.t1

Sequence Viewer

Length: 1257 bp
ATGTCCCAGAAAGTGTTTGATTACAGGCTCCTTAGGCGCACTGTCAAATCCCCCATTTTCACCACCCTAGCATTTGTTGCCATATTCTTCTTCGTCTATGCAGACACCATCGTCTCTAACCTTCCCATGCCTCCTGCTTTCTTGACCTCAACTCATGAACATCATCTTGATGATCACCACACCACCGAGACTGTGAGCATAACGAAACTTGCCATCCAAGAAAAGATTGATGAGTTTACAAATGATGAATTTCTGGATCCACTGATTCCTCCAGACAATGTCACAAAGGAGGAGAGACTAGTGTGGTTCCGGCGGAAGCTCCCAGAGCTCGAGATTCTCCAGTCCAATGATTTTACGCAGAAATTTCACGGTCGAGTCCTTGAGTTCCTCAACAATGGATGCAGCACTCAATTCTACATGATATGGCTTTCACCGGCGAAGTCATTTGGGAAGAGAGAGTTCTTGGCCATGGACACCCTCTTCAATTCTCATCCTAAAGGCTGCTTGATCATCATATCTAGATCCATGGACTCGGAACTGGGATACAGAATCCTGAAGCCACTATTGGATCGGGATTTCAAGATCCTCGCGATCACGCCAGACTTACCATTTTTAGTGAAGAACACACCAGCTGAGTCTTGGCTTGATGAAATGAAGAGTGGGAGGAGAGACCCGGGTAATATTCCATTGTCTCAAAACCTGTCAAATCTTATTAGGCTGGCAATGCTGTATAAGTACGGAGGTGTATACTTGGACACGGACTTTATAATTCTGAAAGATTTCTCTGGTTTGAGGAACGCAGTTGGGGCACAAAGTATAGATTCCAAGTCCAAAAATTGGACTAGACTAAATGGAGCAGTGATGATATTTGACATTCATCATCCACTTTTGAGGGACTTCTTAGATGAATTTGCTTCAACTTATAATGGAAACAAATGGGGTTACAATGGACCATATTTGGTTTCAAGGGTGATCGAGAAAGTGGGAAGTACACCTGGTTATAACCTTACGATTCTGCCTCTTGAGGCATTTTATCCGGTGGACTGGAATAGGATCGGGAGGCTTTATACGAAGCCGAAGACGGATTTGGAGTCGAGATGGGAGGAAAGGATGTTGAATGAGTTGCATGGAGGGGAGACTTATGCCATTCACCTATGGAATAAGAGAAGTAGAATGCTGGCCATTGAAGAGGGAAGTGTCATGGCTAGACTAATCTCAGAGAACTGTGTCATCTGCCAGGACATATATGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

419

Amino Acids

48.28

Weight (kDa)

6.43

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 152 - 271 9.4e-21 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 290 - 413 1.4e-25 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 767, 924, 1002
AasI GACNNNNNNGTC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 837
AccI GTMKAC 1 cut(s) 747
AccII CGCG 1 cut(s) 590
AciI CCGC 1 cut(s) 313
AclWI GGATC 6 cut(s) 251, 264, 516, 576, 577, 1061
AcoI YGGCCR 2 cut(s) 465, 1179
AcsI RAATTY 3 cut(s) 248, 362, 908
AcuI CTGAAG 1 cut(s) 575
AfaI GTAC 2 cut(s) 737, 991
AfiI CCNNNNNNNGG 1 cut(s) 837
AgsI TTSAA 6 cut(s) 484, 580, 918, 966, 1117, 1187
AhlI ACTAGT 1 cut(s) 298
AjnI CCWGG 2 cut(s) 994, 1236
AjuI GAANNNNNNNTTGG 4 cut(s) 548, 580, 1070, 1102
AluBI AGCT 3 cut(s) 319, 328, 632
AluI AGCT 3 cut(s) 319, 328, 632
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 6 cut(s) 118, 182, 289, 663, 696, 1130
AlwI GGATC 6 cut(s) 251, 264, 516, 576, 577, 1061
Ama87I CYCGRG 2 cut(s) 329, 673
AoxI GGCC 2 cut(s) 465, 1179
ApeKI GCWGC 2 cut(s) 402, 501
ApoI RAATTY 3 cut(s) 248, 362, 908
Asp700I GAANNNNTTC 1 cut(s) 779
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 950
AsuC2I CCSGG 2 cut(s) 674, 675
AsuHPI GGTGA 5 cut(s) 52, 167, 423, 982, 1142
AvaI CYCGRG 2 cut(s) 329, 673
AvaII GGWCC 1 cut(s) 950
AxyI CCTNAGG 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 811
BalI TGGCCA 2 cut(s) 467, 1181
BamHI GGATCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 1085
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 414, 488
BccI CCATC 3 cut(s) 116, 221, 1092
BciT130I CCWGG 2 cut(s) 996, 1238
BciVI GTATCC 1 cut(s) 536
BclI TGATCA 2 cut(s) 172, 507
BcnI CCSGG 2 cut(s) 674, 675
BcoDI GTCTC 6 cut(s) 118, 182, 289, 663, 696, 1130
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 5 cut(s) 68, 299, 519, 843, 1206
BfuI GTATCC 1 cut(s) 536
BisI GCNGC 2 cut(s) 403, 502
BlsI GCNGC 2 cut(s) 404, 503
Bme1390I CCNGG 4 cut(s) 674, 675, 996, 1238
Bme18I GGWCC 1 cut(s) 950
BmeT110I CYCGRG 2 cut(s) 329, 673
BmgT120I GGNCC 1 cut(s) 950
BmiI GGNNCC 3 cut(s) 29, 258, 308
BmrFI CCNGG 4 cut(s) 674, 675, 996, 1238
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 1 cut(s) 389
BmuI ACTGGG 1 cut(s) 548
BpiI GAAGAC 1 cut(s) 1085
BpmI CTGGAG 2 cut(s) 255, 323
BpuEI CTTGAG 2 cut(s) 401, 1043
BpuMI CCSGG 2 cut(s) 674, 675
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 3 cut(s) 468, 525, 673
BsaWI WCCGGW 1 cut(s) 1036
Bsc4I CCNNNNNNNGG 1 cut(s) 837
Bse118I RCCGGY 1 cut(s) 433
Bse1I ACTGG 3 cut(s) 340, 543, 1049
Bse21I CCTNAGG 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 2 cut(s) 996, 1238
BseDI CCNNGG 3 cut(s) 468, 525, 673
BseGI GGATG 5 cut(s) 213, 404, 490, 880, 1116
BseLI CCNNNNNNNGG 1 cut(s) 837
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 2 cut(s) 624, 1230
BseNI ACTGG 3 cut(s) 340, 543, 1049
BseRI GAGGAG 2 cut(s) 305, 679
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 2 cut(s) 414, 488
Bsh1236I CGCG 1 cut(s) 590
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 2 cut(s) 467, 1181
BsiEI CGRYCG 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 2 cut(s) 329, 673
BsiSI CCGG 4 cut(s) 310, 434, 674, 1037
BslFI GGGAC 1 cut(s) 908
BslI CCNNNNNNNGG 1 cut(s) 837
BsmAI GTCTC 6 cut(s) 118, 182, 289, 663, 696, 1130
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 908
BsmI GAATGC 1 cut(s) 1179
BsnI GGCC 2 cut(s) 467, 1181
Bso31I GGTCTC 1 cut(s) 663
BsoBI CYCGRG 2 cut(s) 329, 673
Bsp1286I GDGCHC 2 cut(s) 330, 811
Bsp143I GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
Bsp19I CCATGG 2 cut(s) 468, 525
Bsp68I TCGCGA 1 cut(s) 590
BspACI CCGC 1 cut(s) 313
BspANI GGCC 2 cut(s) 467, 1181
BspCNI CTCAG 2 cut(s) 625, 1229
BspFNI CGCG 1 cut(s) 590
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 29, 258, 308
BspPI GGATC 6 cut(s) 251, 264, 516, 576, 577, 1061
BspTNI GGTCTC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 433
BsrI ACTGG 3 cut(s) 340, 543, 1049
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 3 cut(s) 468, 525, 673
BssMI GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
BssNAI GTATAC 1 cut(s) 748
BssT1I CCWWGG 2 cut(s) 468, 525
Bst1107I GTATAC 1 cut(s) 748
Bst2UI CCWGG 2 cut(s) 996, 1238
Bst4CI ACNGT 4 cut(s) 43, 193, 371, 1226
Bst6I CTCTTC 4 cut(s) 446, 485, 650, 1182
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 720, 1179
BstDEI CTNAG 4 cut(s) 32, 633, 901, 1216
BstDSI CCRYGG 2 cut(s) 468, 525
BstF5I GGATG 5 cut(s) 213, 404, 490, 880, 1116
BstFNI CGCG 1 cut(s) 590
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 9 cut(s) 175, 259, 510, 524, 571, 585, 594, 975, 1056
BstMAI GTCTC 6 cut(s) 118, 182, 289, 663, 696, 1130
BstMBI GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 5 cut(s) 34, 77, 325, 724, 806
BstNI CCWGG 2 cut(s) 996, 1238
BstSCI CCNGG 4 cut(s) 672, 673, 994, 1236
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 590
BstV1I GCAGC 2 cut(s) 414, 488
BstV2I GAAGAC 1 cut(s) 1085
BstX2I RGATCY 3 cut(s) 256, 521, 582
BstYI RGATCY 3 cut(s) 256, 521, 582
BstZ17I GTATAC 1 cut(s) 748
Bsu36I CCTNAGG 1 cut(s) 32
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 467, 1181
BtgI CCRYGG 2 cut(s) 468, 525
BtsCI GGATG 5 cut(s) 213, 404, 490, 880, 1116
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 3 cut(s) 39, 260, 864
BtuMI TCGCGA 1 cut(s) 590
Cac8I GCNNGC 2 cut(s) 720, 1179
CciI TCATGA 1 cut(s) 154
CfoI GCGC 1 cut(s) 39
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 950
Cfr9I CCCGGG 1 cut(s) 673
CsiI ACCWGGT 1 cut(s) 994
Csp6I GTAC 2 cut(s) 736, 990
CviAII CATG 7 cut(s) 127, 155, 418, 469, 526, 1127, 1201
CviQI GTAC 2 cut(s) 736, 990
DdeI CTNAG 4 cut(s) 32, 633, 901, 1216
DpnI GATC 9 cut(s) 174, 258, 509, 523, 570, 584, 593, 974, 1055
DpnII GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 465, 1179
Eam1104I CTCTTC 4 cut(s) 446, 485, 650, 1182
EarI CTCTTC 4 cut(s) 446, 485, 650, 1182
EciI GGCGGA 1 cut(s) 328
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 468, 525
Eco24I GRGCYC 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 950
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 575
Eco81I CCTNAGG 1 cut(s) 32
Eco88I CYCGRG 2 cut(s) 329, 673
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 994, 1236
EcoT14I CCWWGG 2 cut(s) 468, 525
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 468, 525
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 7 cut(s) 130, 158, 421, 472, 529, 1130, 1204
FaqI GGGAC 1 cut(s) 908
FatI CATG 7 cut(s) 126, 154, 417, 468, 525, 1126, 1200
FbaI TGATCA 2 cut(s) 172, 507
FblI GTMKAC 1 cut(s) 747
Fnu4HI GCNGC 2 cut(s) 403, 502
FokI GGATG 5 cut(s) 200, 411, 477, 867, 1123
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 403, 502
FspBI CTAG 5 cut(s) 68, 299, 519, 843, 1206
GlaI GCGC 1 cut(s) 38
GluI GCNGC 2 cut(s) 403, 502
GsuI CTGGAG 2 cut(s) 255, 323
HaeIII GGCC 2 cut(s) 467, 1181
HapII CCGG 4 cut(s) 310, 434, 674, 1037
HhaI GCGC 1 cut(s) 39
Hin1II CATG 7 cut(s) 130, 158, 421, 472, 529, 1130, 1204
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 9 cut(s) 265, 334, 375, 530, 549, 635, 821, 1012, 1091
HpaII CCGG 4 cut(s) 310, 434, 674, 1037
HphI GGTGA 5 cut(s) 52, 167, 423, 982, 1142
Hpy166II GTNNAC 4 cut(s) 237, 748, 992, 1042
Hpy188I TCNGA 3 cut(s) 535, 774, 1219
Hpy8I GTNNAC 4 cut(s) 237, 748, 992, 1042
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 4 cut(s) 43, 193, 371, 1226
HpyCH4V TGCA 3 cut(s) 101, 402, 1126
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 77, 325, 724, 806
HpyF3I CTNAG 4 cut(s) 32, 633, 901, 1216
Hsp92II CATG 7 cut(s) 130, 158, 421, 472, 529, 1130, 1204
HspAI GCGC 1 cut(s) 37
Ksp22I TGATCA 2 cut(s) 172, 507
Kzo9I GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
LmnI GCTCC 3 cut(s) 33, 324, 854
Lsp1109I GCAGC 2 cut(s) 414, 488
LweI GCATC 1 cut(s) 389
MabI ACCWGGT 1 cut(s) 994
MaeI CTAG 5 cut(s) 68, 299, 519, 843, 1206
MaeIII GTNAC 2 cut(s) 280, 941
MalI GATC 9 cut(s) 174, 258, 509, 523, 570, 584, 593, 974, 1055
MboI GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
MboII GAAGA 8 cut(s) 79, 82, 463, 472, 631, 667, 1090, 1199
MflI RGATCY 3 cut(s) 256, 521, 582
MhlI GDGCHC 2 cut(s) 330, 811
MlsI TGGCCA 2 cut(s) 467, 1181
MluCI AATT 7 cut(s) 248, 362, 410, 484, 768, 835, 908
MluNI TGGCCA 2 cut(s) 467, 1181
MlyI GAGTC 4 cut(s) 384, 524, 644, 1100
Mox20I TGGCCA 2 cut(s) 467, 1181
MroXI GAANNNNTTC 1 cut(s) 779
MscI TGGCCA 2 cut(s) 467, 1181
MseI TTAA 1 cut(s) 1255
MslI CAYNNNNRTG 1 cut(s) 168
Msp20I TGGCCA 2 cut(s) 467, 1181
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 4 cut(s) 310, 434, 674, 1037
MspR9I CCNGG 4 cut(s) 674, 675, 996, 1238
Mva1269I GAATGC 1 cut(s) 1179
MvaI CCWGG 2 cut(s) 996, 1238
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 5 cut(s) 34, 77, 325, 724, 806
NciI CCSGG 2 cut(s) 674, 675
NcoI CCATGG 2 cut(s) 468, 525
NdeII GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
NlaIII CATG 7 cut(s) 130, 158, 421, 472, 529, 1130, 1204
NlaIV GGNNCC 3 cut(s) 29, 258, 308
NmuCI GTSAC 1 cut(s) 280
NruI TCGCGA 1 cut(s) 590
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 154
PctI GAATGC 1 cut(s) 1179
PdmI GAANNNNTTC 1 cut(s) 779
PfeI GAWTC 5 cut(s) 265, 334, 549, 821, 1012
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 837
PkrI GCNGC 2 cut(s) 404, 503
PleI GAGTC 4 cut(s) 383, 524, 643, 1099
PpsI GAGTC 4 cut(s) 383, 524, 643, 1099
PsiI TTATAA 3 cut(s) 767, 924, 1002
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 994, 1236
PspGI CCWGG 2 cut(s) 994, 1236
PspN4I GGNNCC 3 cut(s) 29, 258, 308
PspPI GGNCC 1 cut(s) 950
PsuI RGATCY 3 cut(s) 256, 521, 582
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 632
RruI TCGCGA 1 cut(s) 590
RsaI GTAC 2 cut(s) 737, 991
RsaNI GTAC 2 cut(s) 736, 990
RseI CAYNNNNRTG 1 cut(s) 168
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 1255
SatI GCNGC 2 cut(s) 403, 502
Sau3AI GATC 9 cut(s) 172, 256, 507, 521, 568, 582, 591, 972, 1053
Sau96I GGNCC 1 cut(s) 950
SchI GAGTC 4 cut(s) 384, 524, 644, 1100
ScrFI CCNGG 4 cut(s) 674, 675, 996, 1238
SduI GDGCHC 2 cut(s) 330, 811
SexAI ACCWGGT 1 cut(s) 994
SfaNI GCATC 1 cut(s) 389
Sfr274I CTCGAG 1 cut(s) 329
SgrAI CRCCGGYG 1 cut(s) 433
SinI GGWCC 1 cut(s) 950
SlaI CTCGAG 1 cut(s) 329
SmaI CCCGGG 1 cut(s) 675
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 3 cut(s) 329, 380, 1022
SmoI CTYRAG 3 cut(s) 329, 380, 1022
SpeI ACTAGT 1 cut(s) 298
Sse9I AATT 7 cut(s) 248, 362, 410, 484, 768, 835, 908
SsiI CCGC 1 cut(s) 313
SspI AATATT 1 cut(s) 682
SspMI CTAG 5 cut(s) 68, 299, 519, 843, 1206
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 4 cut(s) 672, 673, 994, 1236
StyI CCWWGG 2 cut(s) 468, 525
TaaI ACNGT 4 cut(s) 43, 193, 371, 1226
TaqI TCGA 4 cut(s) 330, 373, 975, 1094
TasI AATT 7 cut(s) 248, 362, 410, 484, 768, 835, 908
TatI WGTACW 1 cut(s) 989
TfiI GAWTC 5 cut(s) 265, 334, 549, 821, 1012
Tru1I TTAA 1 cut(s) 1255
Tru9I TTAA 1 cut(s) 1255
TscAI CASTG 3 cut(s) 46, 267, 864
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 402, 501
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 6 cut(s) 171, 261, 663, 668, 866, 921
TspGWI ACGGA 3 cut(s) 753, 773, 1097
TspMI CCCGGG 1 cut(s) 673
TspRI CASTG 3 cut(s) 46, 267, 864
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 837
VpaK11BI GGWCC 1 cut(s) 950
XapI RAATTY 3 cut(s) 248, 362, 908
XbaI TCTAGA 1 cut(s) 518
XcmI CCANNNNNNNNNTGG 1 cut(s) 636
XhoI CTCGAG 1 cut(s) 329
XmaI CCCGGG 1 cut(s) 673
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 1 cut(s) 779
XspI CTAG 5 cut(s) 68, 299, 519, 843, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.