Rorug03G0065700

Glycosyltransferase sugar-binding region containing DXD motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004935
Physical Location & Seq
Forward (+)
4857559 .. 4862369
4811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0065700.1

Sequence Viewer

Length: 2637 bp
ATGGAGATTCAAGCAGAAAACCCGCTGCCGGATAAAGAGAGCAAACGGGTCGGGCAGGGCGGCCCGCTAGGGACCAGCAGCAAGATGAGGAGCAACAGCAACAGAAGCACAATGTTCAAAGAAGAGGATCAATGCTATGTGGAAGTGGCACTCGATGTTTGCGACGACAATAACTTTGTGCTTCGGAACATCAGAGGAGCTGAACCTGAGTCGACGTCAGCAAGGCTAGAGAGAAGACGATCCGTTGCTTCTCAACTCTCTTTTAAACTCAAGAAAGTCACACGTGAACTGAAACGCATGAAATCTTCCTTGAGCTTCAAGAAGGTCGACAGGCCCACCAAGCCCGGTATTGCTCGTGCCCTTAAGGGACTCAAGCTCATGACCAAAAATGTGTGCAGTGAAGGTTGGCCAGAGATCGAAATAAGGTTTGATGAATTGGCTGTCGATGGAACTCTTCCAAAATCAGTCTTTGCTCAATGCATAGGGATGAAAGAGTCGAGTGAGTTTGCTTGCGAAGTATTCGATGCATTGGCTCGTCGGAGAGGAATAACATCGTGTTGTGTGACCAAGGAAGAGTTGAGAGAATTTTGGGAACAAATTTCAGATCACAGCTTCAATGCCAGGCTTCAAACCTTCTTTGACATGGTGGATAAAAATGCAGATGGTCGAATCTCTGAAGAAGAGGTGAAAGAGATTATTACCTTAAGTGCCTCTGCTAATAAGCTGTCAAAAATTCTAGAGCATGCTGAGGAATATGCAGCATTGATTATGGAAGAGCTTGATCATGACAACCTTGGATATATTGATTTGTACAACTTGAAAATGCTACTTCTTCCGGACCCAACCCAATCTTCGAACCTGGAAACAGACAGCTACATTCAGAGCCGGTTGCTAAGTGAAAAGCTGGTTCCGACCAAAGAGCACAACCCTATAAGGCGGTGGTGCCGGCGGCTGTCCTACTTTGTTGAGGATAACTGGAGGCGGATTTGGGTCATTGTGCTATGGCTTTTGATATGCATGGGCCTCTTTGCTTGGAAATTCATTCAGTATAGACAAAGGGAGGTCTTCCATGTAATGGGCTATTGTGTGTGCATTGCCAAAGGTGGAGCTGAGACCCTAAAGTTTAACATGGCCCTGATTCTTCTTCCGGTGTGTAGAAATACAATTACTTGGCTAAGAAGCAAGACCAAGTTAGGAGGTGTAGTTCCATTTGATGACAATATCAATTTTCATAAGGTGATCGCGTTTGGAATTGCAGTTGGGGTCAGTCTCCATGCTGGTTCCCATCTTACATGCGACTTCCCCAGGCTACTACACTCAACCAATGATCAATACGACAAGATGAAGCCATTTTTCGGCCAAAAACGACCCGACAATTACTGGTGGTTTGTGAAGGGAACCGAGGGCTGGACCGGGGTGGTTATGGTGGCACTAATGCTAGTTGCATACACATTGGCTCAACCTTGGTTCCGTCGCAATCGACTAAGAAACCTCCCTAAAACCCTAAAGAAAGTTACTGGGTTCAATGCGTTTTGGTACTCCCATCACCTCTTTATCATTGTGTACATCCTCCTTATTATCCATGGATACTTCCTCTACCTCTCCAAGGACTGGGACAAGAAAACAACATGGATGTATCTGGCTTTCCCAGTAATAATGTATGCATGTGAACGTTTGCTTCGTGCTTTTAGGTCTGGATATGAATCAGTGAAGATCTTAAAGGTTACTGTTTACCCGGGAAACGTATTGGCACTCCATATGTCTAAACCACAGGGATTAAAATATGCCAGTGGACAGTACATTTACATAAATTGTTCTGCTATTTCCTCATTTCAATGGCATCCATTCTCAATTACTTCAGCCCCTGGAGACGACTATCTAAGTGTTCATATTCGCACGTTGGGTGATTGGACATCTCAGCTCAAATACATTTTCTCTCAGGTTTGCGAACGTCCTCCTCTAGAGCTCAGTGGCCTTCTAAGAGCTGATACTGAACAACCCGATATACAACTCAGGATGCCAAGACTTCTTATTGATGGTCCATATGGTGCACCAGCTCAAGACTACAAAAACTACGAAGTTCTTCTACTTGTTGGACTTGGAATTGGAGCAACTCCATTAATAAGCATACTCAAAGATGTGCTTAACAACATTAAGCGACAGAAAGAACTAGAAGCTCAAATGTTGGTGGAAAGTGGTGTTAAGAGAAAGTCTACTTTTGCAACAAAACGTGCTTATTTCTACTGGATCACAAGAGAGCAAGGCTCGTTCGAGTGGTTTAGGGGTGTGATGAATGTGGTCGCAGAAAATGACCTCGACGGCGTGATTGAGCTGCACAACTACTGCACCAGTGTCTATGAAGAAGGAGATGCTAGATCAGCTCTGATTACTATGCTTCAGTCTATAAACCATGCCAAAAATGGTGTGGATGTTGTATCAGGAACGAGGGTGAAGACGCATTTTGCTAGACCAAAATGGCGTAAAGTTTTTAAGCATGTGGCAGTTAAGCATCCTGATCAAAAAGTTGGAGTGTTTTATTGTGGCGCACATGGATTGGTTGGGGAGTTAAAGAGGTTAGCTAAAGACTTTTCCAGAAAAACTGAGACCAAGTTTGATTTCCACAAAGAGAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

878

Amino Acids

100.99

Weight (kDa)

9.16

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 113 - 207 2e-32 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 208 - 270 6.4e-06 EF-hand domain pair
Ferric_reduct PF01794 376 - 526 8.7e-12 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 569 - 686 4.6e-35 FAD-binding domain
NAD_binding_6 PF08030 692 - 860 3.7e-49 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 942
AccB7I CCANNNNNTGG 2 cut(s) 1075, 1611
AccI GTMKAC 3 cut(s) 212, 327, 2213
AccII CGCG 1 cut(s) 1244
AccIII TCCGGA 1 cut(s) 835
AciI CCGC 6 cut(s) 23, 60, 65, 937, 949, 982
AclI AACGTT 1 cut(s) 1672
AclWI GGATC 3 cut(s) 135, 234, 2255
AcoI YGGCCR 2 cut(s) 407, 1357
AcsI RAATTY 4 cut(s) 584, 597, 732, 1037
AcuI CTGAAG 3 cut(s) 696, 1842, 2381
AcvI CACGTG 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 4 cut(s) 812, 1538, 1565, 1799
AfiI CCNNNNNNNGG 6 cut(s) 28, 1075, 1355, 1407, 1606, 1611
AflII CTTAAG 2 cut(s) 362, 703
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 8 cut(s) 11, 118, 319, 616, 629, 820, 1525, 1835
AjnI CCWGG 4 cut(s) 620, 858, 1304, 1864
AjuI GAANNNNNNNTTGG 4 cut(s) 835, 867, 2600, 2632
AloI GAACNNNNNNTCC 2 cut(s) 1188, 1220
Alw21I GWGCWC 3 cut(s) 924, 1968, 2053
Alw26I GTCTC 4 cut(s) 1106, 1274, 1863, 2597
Alw44I GTGCAC 1 cut(s) 2049
AlwI GGATC 3 cut(s) 135, 234, 2255
AlwNI CAGNNNCTG 1 cut(s) 1865
Ama87I CYCGRG 1 cut(s) 1735
Aor13HI TCCGGA 1 cut(s) 835
AoxI GGCC 7 cut(s) 61, 332, 407, 1021, 1131, 1357, 1972
ApaLI GTGCAC 1 cut(s) 2049
ApeKI GCWGC 4 cut(s) 25, 78, 758, 2332
ApoI RAATTY 4 cut(s) 584, 597, 732, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AseI ATTAAT 1 cut(s) 2120
Asp700I GAANNNNTTC 1 cut(s) 2082
AspLEI GCGC 1 cut(s) 2546
AspS9I GGNCC 8 cut(s) 62, 72, 333, 838, 1021, 1132, 1410, 2039
AsuC2I CCSGG 4 cut(s) 345, 1414, 1736, 1737
AsuHPI GGTGA 5 cut(s) 697, 1249, 1538, 1916, 2461
AsuII TTCGAA 1 cut(s) 854
AvaI CYCGRG 1 cut(s) 1735
AvaII GGWCC 4 cut(s) 72, 838, 1410, 2039
BaeGI GKGCMC 2 cut(s) 361, 2053
BalI TGGCCA 1 cut(s) 409
BanI GGYRCC 1 cut(s) 942
BanII GRGCYC 1 cut(s) 1968
BarI GAAGNNNNNNTAC 2 cut(s) 2070, 2102
BauI CACGAG 1 cut(s) 354
BbrPI CACGTG 1 cut(s) 284
BbsI GAAGAC 3 cut(s) 241, 1057, 2459
Bbv12I GWGCWC 3 cut(s) 924, 1968, 2053
BbvCI CCTCAGC 1 cut(s) 747
BbvI GCAGC 4 cut(s) 12, 90, 770, 2319
BccI CCATC 5 cut(s) 440, 656, 1293, 1551, 2030
BceAI ACGGC 1 cut(s) 2335
BcgI CGANNNNNNTGC 2 cut(s) 31, 65
BciT130I CCWGG 4 cut(s) 622, 860, 1306, 1866
BciVI GTATCC 1 cut(s) 1580
BclI TGATCA 3 cut(s) 781, 1327, 2515
BcnI CCSGG 4 cut(s) 345, 1414, 1736, 1737
BcoDI GTCTC 4 cut(s) 1106, 1274, 1863, 2597
BfaI CTAG 8 cut(s) 68, 227, 737, 1439, 1961, 2171, 2373, 2466
BfrI CTTAAG 2 cut(s) 362, 703
BfuI GTATCC 1 cut(s) 1580
BglII AGATCT 1 cut(s) 1713
BisI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
BlsI GCNGC 6 cut(s) 27, 62, 80, 760, 951, 2334
Bme1390I CCNGG 8 cut(s) 345, 622, 860, 1306, 1414, 1736, 1737, 1866
Bme18I GGWCC 4 cut(s) 72, 838, 1410, 2039
BmeT110I CYCGRG 1 cut(s) 1735
BmgT120I GGNCC 8 cut(s) 62, 72, 333, 838, 1021, 1132, 1410, 2039
BmiI GGNNCC 7 cut(s) 73, 840, 909, 944, 1282, 1399, 1469
BmrFI CCNGG 8 cut(s) 345, 622, 860, 1306, 1414, 1736, 1737, 1866
BmrI ACTGGG 3 cut(s) 1527, 1621, 1643
BmsI GCATC 5 cut(s) 514, 1849, 2007, 2359, 2518
BmuI ACTGGG 3 cut(s) 1527, 1621, 1643
BpiI GAAGAC 3 cut(s) 241, 1057, 2459
BplI GAGNNNNNCTC 2 cut(s) 2249, 2281
BpmI CTGGAG 2 cut(s) 997, 1887
Bpu10I CCTNAGC 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 854
BpuEI CTTGAG 4 cut(s) 254, 331, 356, 2043
BpuMI CCSGG 4 cut(s) 345, 1414, 1736, 1737
BsaAI YACGTR 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 1702
BsaHI GRCGYC 1 cut(s) 215
BsaI GGTCTC 2 cut(s) 1106, 2597
BsaWI WCCGGW 2 cut(s) 835, 1147
BsaXI ACNNNNNCTCC 2 cut(s) 1188, 1218
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 1075, 1355, 1407, 1606, 1611
Bse118I RCCGGY 2 cut(s) 885, 945
Bse1I ACTGG 8 cut(s) 980, 1385, 1522, 1616, 1649, 1788, 2249, 2349
Bse3DI GCAATG 1 cut(s) 1092
Bse8I GATNNNNATC 1 cut(s) 1702
BseAI TCCGGA 1 cut(s) 835
BseBI CCWGG 4 cut(s) 622, 860, 1306, 1866
BseGI GGATG 7 cut(s) 492, 1566, 1638, 1840, 2022, 2434, 2509
BseJI GATNNNNATC 1 cut(s) 1702
BseLI CCNNNNNNNGG 6 cut(s) 28, 1075, 1355, 1407, 1606, 1611
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 8 cut(s) 198, 738, 1101, 1931, 1952, 1981, 2026, 2592
BseNI ACTGG 8 cut(s) 980, 1385, 1522, 1616, 1649, 1788, 2249, 2349
BseRI GAGGAG 3 cut(s) 103, 210, 1947
BseSI GKGCMC 2 cut(s) 361, 2053
BseXI GCAGC 4 cut(s) 12, 90, 770, 2319
BsgI GTGCAG 3 cut(s) 415, 2318, 2329
Bsh1236I CGCG 1 cut(s) 1244
BshFI GGCC 7 cut(s) 63, 334, 409, 1023, 1133, 1359, 1974
BshNI GGYRCC 1 cut(s) 942
BsiHKAI GWGCWC 3 cut(s) 924, 1968, 2053
BsiHKCI CYCGRG 1 cut(s) 1735
BsiSI CCGG 8 cut(s) 29, 345, 836, 886, 946, 1148, 1413, 1736
BslFI GGGAC 3 cut(s) 85, 381, 1628
BslI CCNNNNNNNGG 6 cut(s) 28, 1075, 1355, 1407, 1606, 1611
BsmAI GTCTC 4 cut(s) 1106, 1274, 1863, 2597
BsmBI CGTCTC 1 cut(s) 1863
BsmFI GGGAC 3 cut(s) 85, 381, 1628
BsnI GGCC 7 cut(s) 63, 334, 409, 1023, 1133, 1359, 1974
Bso31I GGTCTC 2 cut(s) 1106, 2597
BsoBI CYCGRG 1 cut(s) 1735
Bsp119I TTCGAA 1 cut(s) 854
Bsp1286I GDGCHC 4 cut(s) 361, 924, 1968, 2053
Bsp13I TCCGGA 1 cut(s) 835
Bsp1407I TGTACA 2 cut(s) 810, 1563
Bsp19I CCATGG 1 cut(s) 1582
BspACI CCGC 6 cut(s) 23, 60, 65, 937, 949, 982
BspANI GGCC 7 cut(s) 63, 334, 409, 1023, 1133, 1359, 1974
BspCNI CTCAG 8 cut(s) 199, 739, 1102, 1930, 1951, 1980, 2025, 2593
BspEI TCCGGA 1 cut(s) 835
BspFNI CGCG 1 cut(s) 1244
BspHI TCATGA 2 cut(s) 378, 784
BspLI GGNNCC 7 cut(s) 73, 840, 909, 944, 1282, 1399, 1469
BspPI GGATC 3 cut(s) 135, 234, 2255
BspQI GCTCTTC 1 cut(s) 768
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 1 cut(s) 942
BspTI CTTAAG 2 cut(s) 362, 703
BspTNI GGTCTC 2 cut(s) 1106, 2597
BsrDI GCAATG 1 cut(s) 1092
BsrFI RCCGGY 2 cut(s) 885, 945
BsrGI TGTACA 2 cut(s) 810, 1563
BsrI ACTGG 8 cut(s) 980, 1385, 1522, 1616, 1649, 1788, 2249, 2349
BssAI RCCGGY 2 cut(s) 885, 945
BssNI GRCGYC 1 cut(s) 215
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 5 cut(s) 567, 793, 1463, 1582, 1605
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 4 cut(s) 622, 860, 1306, 1866
Bst4CI ACNGT 2 cut(s) 1729, 1797
Bst6I CTCTTC 5 cut(s) 117, 459, 567, 675, 768
BstACI GRCGYC 1 cut(s) 215
BstAFI CTTAAG 2 cut(s) 362, 703
BstAUI TGTACA 2 cut(s) 810, 1563
BstBAI YACGTR 1 cut(s) 284
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 4 cut(s) 65, 511, 744, 947
BstDSI CCRYGG 1 cut(s) 1582
BstENI CCTNNNNNAGG 1 cut(s) 1604
BstF5I GGATG 7 cut(s) 492, 1566, 1638, 1840, 2022, 2434, 2509
BstFNI CGCG 1 cut(s) 1244
BstHHI GCGC 1 cut(s) 2546
BstMAI GTCTC 4 cut(s) 1106, 1274, 1863, 2597
BstMWI GCNNNNNNNGC 3 cut(s) 105, 340, 2378
BstNI CCWGG 4 cut(s) 622, 860, 1306, 1866
BstNSI RCATGY 4 cut(s) 746, 1296, 1668, 2498
BstSCI CCNGG 8 cut(s) 343, 620, 858, 1304, 1412, 1734, 1735, 1864
BstSLI GKGCMC 2 cut(s) 361, 2053
BstUI CGCG 1 cut(s) 1244
BstV1I GCAGC 4 cut(s) 12, 90, 770, 2319
BstV2I GAAGAC 3 cut(s) 241, 1057, 2459
BstX2I RGATCY 1 cut(s) 1713
BstYI RGATCY 1 cut(s) 1713
BsuI GTATCC 1 cut(s) 1580
BsuRI GGCC 7 cut(s) 63, 334, 409, 1023, 1133, 1359, 1974
BtgI CCRYGG 1 cut(s) 1582
BtsCI GGATG 7 cut(s) 492, 1566, 1638, 1840, 2022, 2434, 2509
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 5 cut(s) 403, 1713, 1795, 1975, 2356
Cac8I GCNNGC 4 cut(s) 65, 511, 744, 947
CaiI CAGNNNCTG 1 cut(s) 1865
CciI TCATGA 2 cut(s) 378, 784
CfoI GCGC 1 cut(s) 2546
Cfr10I RCCGGY 2 cut(s) 885, 945
Cfr13I GGNCC 8 cut(s) 62, 72, 333, 838, 1021, 1132, 1410, 2039
Cfr9I CCCGGG 1 cut(s) 1735
CseI GACGC 1 cut(s) 2464
Csp6I GTAC 4 cut(s) 811, 1537, 1564, 1798
CviQI GTAC 4 cut(s) 811, 1537, 1564, 1798
DraI TTTAAA 1 cut(s) 265
EaeI YGGCCR 2 cut(s) 407, 1357
Eam1104I CTCTTC 5 cut(s) 117, 459, 567, 675, 768
EarI CTCTTC 5 cut(s) 117, 459, 567, 675, 768
EciI GGCGGA 1 cut(s) 997
Ecl136II GAGCTC 1 cut(s) 1966
Eco130I CCWWGG 5 cut(s) 567, 793, 1463, 1582, 1605
Eco24I GRGCYC 1 cut(s) 1968
Eco31I GGTCTC 2 cut(s) 1106, 2597
Eco47I GGWCC 4 cut(s) 72, 838, 1410, 2039
Eco53kI GAGCTC 1 cut(s) 1966
Eco57I CTGAAG 3 cut(s) 696, 1842, 2381
Eco72I CACGTG 1 cut(s) 284
Eco88I CYCGRG 1 cut(s) 1735
EcoICRI GAGCTC 1 cut(s) 1966
EcoNI CCTNNNNNAGG 1 cut(s) 1604
EcoRII CCWGG 4 cut(s) 620, 858, 1304, 1864
EcoT14I CCWWGG 5 cut(s) 567, 793, 1463, 1582, 1605
EcoT22I ATGCAT 4 cut(s) 482, 529, 1019, 1666
EcoT38I GRGCYC 1 cut(s) 1968
ErhI CCWWGG 5 cut(s) 567, 793, 1463, 1582, 1605
Esp3I CGTCTC 1 cut(s) 1863
FalI AAGNNNNNCTT 2 cut(s) 2127, 2159
FaqI GGGAC 3 cut(s) 85, 381, 1628
FauI CCCGC 2 cut(s) 30, 72
FauNDI CATATG 2 cut(s) 1758, 2044
FbaI TGATCA 3 cut(s) 781, 1327, 2515
FblI GTMKAC 3 cut(s) 212, 327, 2213
Fnu4HI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
FokI GGATG 7 cut(s) 499, 1553, 1645, 1827, 2029, 2441, 2496
FriOI GRGCYC 1 cut(s) 1968
Fsp4HI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
FspBI CTAG 8 cut(s) 68, 227, 737, 1439, 1961, 2171, 2373, 2466
GlaI GCGC 1 cut(s) 2545
GluI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
GsuI CTGGAG 2 cut(s) 997, 1887
HaeIII GGCC 7 cut(s) 63, 334, 409, 1023, 1133, 1359, 1974
HapII CCGG 8 cut(s) 29, 345, 836, 886, 946, 1148, 1413, 1736
HgaI GACGC 1 cut(s) 2464
HhaI GCGC 1 cut(s) 2546
Hin1I GRCGYC 1 cut(s) 215
Hin6I GCGC 1 cut(s) 2544
HinP1I GCGC 1 cut(s) 2544
HincII GTYRAC 2 cut(s) 213, 328
HindII GTYRAC 2 cut(s) 213, 328
HinfI GANTC 7 cut(s) 7, 209, 369, 494, 669, 1138, 1703
HpaII CCGG 8 cut(s) 29, 345, 836, 886, 946, 1148, 1413, 1736
HphI GGTGA 5 cut(s) 697, 1249, 1538, 1916, 2461
Hpy166II GTNNAC 9 cut(s) 213, 287, 328, 1564, 1670, 1732, 1793, 2051, 2214
Hpy188I TCNGA 8 cut(s) 186, 194, 540, 604, 676, 882, 912, 2385
Hpy8I GTNNAC 9 cut(s) 213, 287, 328, 1564, 1670, 1732, 1793, 2051, 2214
Hpy99I CGWCG 5 cut(s) 167, 217, 540, 1476, 2321
HpyAV CCTTC 6 cut(s) 316, 395, 643, 1387, 1985, 2357
HpyCH4III ACNGT 2 cut(s) 1729, 1797
HpyCH4IV ACGT 7 cut(s) 215, 283, 1672, 1743, 1898, 1951, 2230
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 340, 2378
HpySE526I ACGT 7 cut(s) 215, 283, 1672, 1743, 1898, 1951, 2230
Hsp92I GRCGYC 1 cut(s) 215
HspAI GCGC 1 cut(s) 2544
Kpn2I TCCGGA 1 cut(s) 835
KroI GCCGGC 1 cut(s) 945
KroNI GCCGGC 1 cut(s) 947
Ksp22I TGATCA 3 cut(s) 781, 1327, 2515
LguI GCTCTTC 1 cut(s) 768
LmnI GCTCC 4 cut(s) 90, 197, 1106, 2108
Lsp1109I GCAGC 4 cut(s) 12, 90, 770, 2319
LweI GCATC 5 cut(s) 514, 1849, 2007, 2359, 2518
MaeI CTAG 8 cut(s) 68, 227, 737, 1439, 1961, 2171, 2373, 2466
MaeII ACGT 7 cut(s) 215, 283, 1672, 1743, 1898, 1951, 2230
MaeIII GTNAC 4 cut(s) 277, 562, 1513, 1723
MflI RGATCY 1 cut(s) 1713
MhlI GDGCHC 4 cut(s) 361, 924, 1968, 2053
MlsI TGGCCA 1 cut(s) 409
MluNI TGGCCA 1 cut(s) 409
MlyI GAGTC 3 cut(s) 218, 363, 503
MmeI TCCRAC 4 cut(s) 518, 935, 2074, 2506
Mox20I TGGCCA 1 cut(s) 409
Mph1103I ATGCAT 4 cut(s) 482, 529, 1019, 1666
MroI TCCGGA 1 cut(s) 835
MroNI GCCGGC 1 cut(s) 945
MroXI GAANNNNTTC 1 cut(s) 2082
MscI TGGCCA 1 cut(s) 409
MslI CAYNNNNRTG 2 cut(s) 485, 1834
Msp20I TGGCCA 1 cut(s) 409
MspA1I CMGCKG 1 cut(s) 25
MspCI CTTAAG 2 cut(s) 362, 703
MspI CCGG 8 cut(s) 29, 345, 836, 886, 946, 1148, 1413, 1736
MspR9I CCNGG 8 cut(s) 345, 622, 860, 1306, 1414, 1736, 1737, 1866
MvaI CCWGG 4 cut(s) 622, 860, 1306, 1866
MvnI CGCG 1 cut(s) 1244
MwoI GCNNNNNNNGC 3 cut(s) 105, 340, 2378
NaeI GCCGGC 1 cut(s) 947
NciI CCSGG 4 cut(s) 345, 1414, 1736, 1737
NcoI CCATGG 1 cut(s) 1582
NdeI CATATG 2 cut(s) 1758, 2044
NgoMIV GCCGGC 1 cut(s) 945
NlaIV GGNNCC 7 cut(s) 73, 840, 909, 944, 1282, 1399, 1469
NmuCI GTSAC 2 cut(s) 277, 562
NsiI ATGCAT 4 cut(s) 482, 529, 1019, 1666
NspI RCATGY 4 cut(s) 746, 1296, 1668, 2498
NspV TTCGAA 1 cut(s) 854
PaeI GCATGC 1 cut(s) 746
PagI TCATGA 2 cut(s) 378, 784
PciSI GCTCTTC 1 cut(s) 768
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 1678
PdiI GCCGGC 1 cut(s) 947
PdmI GAANNNNTTC 1 cut(s) 2082
PfeI GAWTC 4 cut(s) 7, 669, 1138, 1703
PflMI CCANNNNNTGG 2 cut(s) 1075, 1611
PkrI GCNGC 6 cut(s) 27, 62, 80, 760, 951, 2334
PleI GAGTC 3 cut(s) 217, 363, 502
PmaCI CACGTG 1 cut(s) 284
PmlI CACGTG 1 cut(s) 284
PpsI GAGTC 3 cut(s) 217, 363, 502
Ppu21I YACGTR 1 cut(s) 284
PshBI ATTAAT 1 cut(s) 2120
Psp124BI GAGCTC 1 cut(s) 1968
Psp1406I AACGTT 1 cut(s) 1672
Psp6I CCWGG 4 cut(s) 620, 858, 1304, 1864
PspCI CACGTG 1 cut(s) 284
PspGI CCWGG 4 cut(s) 620, 858, 1304, 1864
PspN4I GGNNCC 7 cut(s) 73, 840, 909, 944, 1282, 1399, 1469
PspPI GGNCC 8 cut(s) 62, 72, 333, 838, 1021, 1132, 1410, 2039
PstNI CAGNNNCTG 1 cut(s) 1865
PsuI RGATCY 1 cut(s) 1713
RsaI GTAC 4 cut(s) 812, 1538, 1565, 1799
RsaNI GTAC 4 cut(s) 811, 1537, 1564, 1798
RseI CAYNNNNRTG 2 cut(s) 485, 1834
SacI GAGCTC 1 cut(s) 1968
SalI GTCGAC 2 cut(s) 211, 326
SapI GCTCTTC 1 cut(s) 768
SatI GCNGC 6 cut(s) 26, 61, 79, 759, 950, 2333
Sau96I GGNCC 8 cut(s) 62, 72, 333, 838, 1021, 1132, 1410, 2039
SchI GAGTC 3 cut(s) 218, 363, 503
ScrFI CCNGG 8 cut(s) 345, 622, 860, 1306, 1414, 1736, 1737, 1866
SduI GDGCHC 4 cut(s) 361, 924, 1968, 2053
SfaNI GCATC 5 cut(s) 514, 1849, 2007, 2359, 2518
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 4 cut(s) 72, 838, 1410, 2039
SmaI CCCGGG 1 cut(s) 1737
SmiMI CAYNNNNRTG 2 cut(s) 485, 1834
SmlI CTYRAG 6 cut(s) 269, 310, 362, 371, 703, 2058
SmoI CTYRAG 6 cut(s) 269, 310, 362, 371, 703, 2058
SphI GCATGC 1 cut(s) 746
SsiI CCGC 6 cut(s) 23, 60, 65, 937, 949, 982
SspMI CTAG 8 cut(s) 68, 227, 737, 1439, 1961, 2171, 2373, 2466
SstI GAGCTC 1 cut(s) 1968
StyD4I CCNGG 8 cut(s) 343, 620, 858, 1304, 1412, 1734, 1735, 1864
StyI CCWWGG 5 cut(s) 567, 793, 1463, 1582, 1605
TaaI ACNGT 2 cut(s) 1729, 1797
TaiI ACGT 7 cut(s) 218, 286, 1675, 1746, 1901, 1954, 2233
TatI WGTACW 3 cut(s) 810, 1563, 1797
TauI GCSGC 2 cut(s) 63, 952
TfiI GAWTC 4 cut(s) 7, 669, 1138, 1703
TscAI CASTG 5 cut(s) 403, 1713, 1795, 1975, 2356
TseFI GTSAC 2 cut(s) 277, 562
TseI GCWGC 4 cut(s) 25, 78, 758, 2332
Tsp45I GTSAC 2 cut(s) 277, 562
TspGWI ACGGA 2 cut(s) 232, 1460
TspMI CCCGGG 1 cut(s) 1735
TspRI CASTG 5 cut(s) 403, 1713, 1795, 1975, 2356
Van91I CCANNNNNTGG 2 cut(s) 1075, 1611
Vha464I CTTAAG 2 cut(s) 362, 703
VneI GTGCAC 1 cut(s) 2049
VpaK11BI GGWCC 4 cut(s) 72, 838, 1410, 2039
VspI ATTAAT 1 cut(s) 2120
XagI CCTNNNNNAGG 1 cut(s) 1604
XapI RAATTY 4 cut(s) 584, 597, 732, 1037
XbaI TCTAGA 2 cut(s) 736, 1960
XceI RCATGY 4 cut(s) 746, 1296, 1668, 2498
XcmI CCANNNNNNNNNTGG 2 cut(s) 2417, 2422
XmaI CCCGGG 1 cut(s) 1735
XmiI GTMKAC 3 cut(s) 212, 327, 2213
XmnI GAANNNNTTC 1 cut(s) 2082
XspI CTAG 8 cut(s) 68, 227, 737, 1439, 1961, 2171, 2373, 2466
ZraI GACGTC 1 cut(s) 216
Zsp2I ATGCAT 4 cut(s) 482, 529, 1019, 1666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.