Rroxscaffold_6G00416490

Glycosyltransferase sugar-binding region containing DXD motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004935
Physical Location & Seq
Reverse (-)
38521422 .. 38522675
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416490.1

Sequence Viewer

Length: 1254 bp
ATGAAAAGAATATCGCAAAAACTGTTTCATTATAACCTATTTAGACGCACTGCCAAAACTTCTATTTTCTCCACCATTTCATTTGCTGCCATATTCTTTCTCCTATATTCATCAGACACCATAGTCTCGATCTCTAACCTTCCCATGCATCCGGTTGACCTCAAAAGTCATAAAATTGTTGACCATCACCAGGAACCAACCTTGATTACTACTACTAATGTCCAAGAAGAGATCAGCATTGATGAAGGTCCAGATGAAGTTTACGACCCTCTAATCCCTCCCGATAATGTAACAAAAGAAGAAAGACAAGTGTGGTTCAGGCGGAAGCTTCCAGAGCTGGAGATACTCCGGTCCAATAGCTTGACGCAGCAATTCCACAGTCGAGTTCTCCAGTTTCTCAACAACGGATGCAGCATTCAATTCTACATGATATGGTTTTCACCAGCGAAGTCATTCGGAAAAAGAGAATTCTTCGCCATGGACAGCCTCTTCAAAGCTCATCCTCAAGGATGTTTGATGCTATTATCGAAAAGCATGGATTCGGGACGCGGATACCGAATCCTGAAACCATTACTTGATGGAGGATTCAAAATACTCGCGGTGACTCCAGACTTGCCATTTCTAGTCAAAAACACTCCAGGTGAAATTTGGCTTGGCGAAATGAAGAGTGGGAGGAAAGACCCAGGTTACATCCCATTGTCCCAAAACCTAGCCAATCTGATCAGACTTGCCGTGCTTTACAAGTATGGAGGTGTATACTTAGACACAGACTTCATAACTCTGAAGGATTTCTCGGGGTTGAGGAATGCAATTGGAGCACAAAACGTAGATTTAGAGTCCAAAAACTGGACCATACTAAACAATGCAGTCATGATCTTCGACATAAACCATCCGCTTCTACTAGACTTCATGGGAGAATTTGCTTCAACATTTAATGGAAACAAATGGGGTCACAACGGACCTTACTTGGTCTCCAGGGTAACTGAGAAAATGGGGCGCACACCGGGTTACAACCTTACAATTTTGCCTCCCGAGGCGTTTTATCCGGTGGAATGGAATAAAATTCGGAGGATTTTCCGGAAGCCTCAGAAGGAATCGGAGTCGAGATGGGTGGAAAGCATGTTGAATGAGTTGAATGAAGGGGAGACTTATGCTCTACACCTATGGAACAAGAGAAGTAGAGAATTGACCATTGAAGAGGGAAGTGTCATGGCTAGACTAATATCGGCGCATTGTGTTATTTGCCGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

417

Amino Acids

47.98

Weight (kDa)

8.83

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 155 - 275 1.5e-21 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 293 - 415 3.8e-27 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 33
AasI GACNNNNNNGTC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 846
AccI GTMKAC 1 cut(s) 756
AccII CGCG 2 cut(s) 549, 599
AccIII TCCGGA 1 cut(s) 1077
AciI CCGC 4 cut(s) 322, 549, 599, 893
AcsI RAATTY 4 cut(s) 467, 645, 917, 1062
AcuI CTGAAG 1 cut(s) 803
AfiI CCNNNNNNNGG 2 cut(s) 190, 846
AgsI TTSAA 7 cut(s) 419, 493, 589, 927, 1126, 1135, 1196
AjnI CCWGG 4 cut(s) 189, 637, 682, 974
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 4 cut(s) 328, 337, 360, 497
AluI AGCT 4 cut(s) 328, 337, 360, 497
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 3 cut(s) 130, 976, 1139
Ama87I CYCGRG 2 cut(s) 793, 1031
Aor13HI TCCGGA 1 cut(s) 1077
ApeKI GCWGC 3 cut(s) 86, 367, 411
ApoI RAATTY 4 cut(s) 467, 645, 917, 1062
Asp700I GAANNNNTTC 2 cut(s) 452, 788
AspLEI GCGC 2 cut(s) 999, 1231
AspS9I GGNCC 4 cut(s) 248, 351, 849, 959
AsuC2I CCSGG 1 cut(s) 1005
AsuHPI GGTGA 4 cut(s) 179, 432, 613, 653
AvaI CYCGRG 2 cut(s) 793, 1031
AvaII GGWCC 4 cut(s) 248, 351, 849, 959
BaeI ACNNNNGTAYC 2 cut(s) 335, 368
Bbv12I GWGCWC 1 cut(s) 820
BbvI GCAGC 3 cut(s) 73, 379, 423
BccI CCATC 4 cut(s) 192, 572, 897, 1101
BceAI ACGGC 1 cut(s) 716
BciT130I CCWGG 4 cut(s) 191, 639, 684, 976
BciVI GTATCC 1 cut(s) 545
BclI TGATCA 1 cut(s) 720
BcnI CCSGG 1 cut(s) 1005
BcoDI GTCTC 3 cut(s) 130, 976, 1139
BfaI CTAG 4 cut(s) 623, 710, 902, 1215
BfuI GTATCC 1 cut(s) 545
BisI GCNGC 3 cut(s) 87, 368, 412
BlsI GCNGC 3 cut(s) 88, 369, 413
Bme1390I CCNGG 5 cut(s) 191, 639, 684, 976, 1005
Bme18I GGWCC 4 cut(s) 248, 351, 849, 959
BmeT110I CYCGRG 2 cut(s) 793, 1031
BmgT120I GGNCC 4 cut(s) 248, 351, 849, 959
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 5 cut(s) 191, 639, 684, 976, 1005
BmsI GCATC 3 cut(s) 157, 398, 507
BpmI CTGGAG 5 cut(s) 359, 374, 591, 621, 958
BpuEI CTTGAG 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 1005
BsaI GGTCTC 1 cut(s) 976
BsaJI CCNNGG 4 cut(s) 477, 682, 975, 1032
BsaWI WCCGGW 4 cut(s) 151, 348, 1045, 1077
BsaXI ACNNNNNCTCC 2 cut(s) 956, 986
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 846
Bse1I ACTGG 2 cut(s) 391, 851
BseAI TCCGGA 1 cut(s) 1077
BseBI CCWGG 4 cut(s) 191, 639, 684, 976
BseDI CCNNGG 4 cut(s) 477, 682, 975, 1032
BseGI GGATG 6 cut(s) 148, 413, 499, 515, 690, 889
BseLI CCNNNNNNNGG 2 cut(s) 190, 846
BseMII CTCAG 2 cut(s) 975, 1100
BseNI ACTGG 2 cut(s) 391, 851
BseXI GCAGC 3 cut(s) 73, 379, 423
Bsh1236I CGCG 2 cut(s) 549, 599
BsiHKAI GWGCWC 1 cut(s) 820
BsiHKCI CYCGRG 2 cut(s) 793, 1031
BsiSI CCGG 6 cut(s) 152, 349, 1004, 1046, 1078, 1246
BslFI GGGAC 2 cut(s) 558, 685
BslI CCNNNNNNNGG 2 cut(s) 190, 846
BsmAI GTCTC 3 cut(s) 130, 976, 1139
BsmFI GGGAC 2 cut(s) 558, 685
BsmI GAATGC 2 cut(s) 414, 811
Bso31I GGTCTC 1 cut(s) 976
BsoBI CYCGRG 2 cut(s) 793, 1031
Bsp1286I GDGCHC 1 cut(s) 820
Bsp13I TCCGGA 1 cut(s) 1077
Bsp143I GATC 4 cut(s) 129, 231, 720, 873
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 4 cut(s) 322, 549, 599, 893
BspCNI CTCAG 2 cut(s) 976, 1099
BspEI TCCGGA 1 cut(s) 1077
BspFNI CGCG 2 cut(s) 549, 599
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 1 cut(s) 195
BspTNI GGTCTC 1 cut(s) 976
BsrI ACTGG 2 cut(s) 391, 851
BssECI CCNNGG 4 cut(s) 477, 682, 975, 1032
BssMI GATC 4 cut(s) 129, 231, 720, 873
BssNAI GTATAC 1 cut(s) 757
BssT1I CCWWGG 1 cut(s) 477
Bst1107I GTATAC 1 cut(s) 757
Bst2UI CCWGG 4 cut(s) 191, 639, 684, 976
Bst4CI ACNGT 2 cut(s) 24, 380
Bst6I CTCTTC 4 cut(s) 222, 494, 659, 1191
BstDEI CTNAG 3 cut(s) 760, 984, 1086
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 6 cut(s) 148, 413, 499, 515, 690, 889
BstFNI CGCG 2 cut(s) 549, 599
BstHHI GCGC 2 cut(s) 999, 1231
BstKTI GATC 4 cut(s) 132, 234, 723, 876
BstMAI GTCTC 3 cut(s) 130, 976, 1139
BstMBI GATC 4 cut(s) 129, 231, 720, 873
BstMWI GCNNNNNNNGC 2 cut(s) 334, 815
BstNI CCWGG 4 cut(s) 191, 639, 684, 976
BstNSI RCATGY 1 cut(s) 1123
BstSCI CCNGG 5 cut(s) 189, 637, 682, 974, 1003
BstUI CGCG 2 cut(s) 549, 599
BstV1I GCAGC 3 cut(s) 73, 379, 423
BstZ17I GTATAC 1 cut(s) 757
BsuI GTATCC 1 cut(s) 545
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 6 cut(s) 148, 413, 499, 515, 690, 889
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
CciI TCATGA 1 cut(s) 870
CfoI GCGC 2 cut(s) 999, 1231
Cfr13I GGNCC 4 cut(s) 248, 351, 849, 959
CseI GACGC 3 cut(s) 54, 373, 555
CviAII CATG 8 cut(s) 145, 427, 478, 535, 871, 910, 1120, 1210
CviJI RGCY 9 cut(s) 328, 337, 360, 486, 497, 652, 713, 1084, 1214
CviKI_1 RGCY 9 cut(s) 328, 337, 360, 486, 497, 652, 713, 1084, 1214
DdeI CTNAG 3 cut(s) 760, 984, 1086
DpnI GATC 4 cut(s) 131, 233, 722, 875
DpnII GATC 4 cut(s) 129, 231, 720, 873
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 4 cut(s) 222, 494, 659, 1191
EarI CTCTTC 4 cut(s) 222, 494, 659, 1191
EciI GGCGGA 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 477
Eco31I GGTCTC 1 cut(s) 976
Eco47I GGWCC 4 cut(s) 248, 351, 849, 959
Eco57I CTGAAG 1 cut(s) 803
Eco88I CYCGRG 2 cut(s) 793, 1031
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 4 cut(s) 189, 637, 682, 974
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 8 cut(s) 148, 430, 481, 538, 874, 913, 1123, 1213
FaqI GGGAC 2 cut(s) 558, 685
FatI CATG 8 cut(s) 144, 426, 477, 534, 870, 909, 1119, 1209
FbaI TGATCA 1 cut(s) 720
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 3 cut(s) 87, 368, 412
FokI GGATG 6 cut(s) 135, 420, 486, 522, 677, 876
Fsp4HI GCNGC 3 cut(s) 87, 368, 412
FspBI CTAG 4 cut(s) 623, 710, 902, 1215
GlaI GCGC 2 cut(s) 998, 1230
GluI GCNGC 3 cut(s) 87, 368, 412
GsuI CTGGAG 5 cut(s) 359, 374, 591, 621, 958
HapII CCGG 6 cut(s) 152, 349, 1004, 1046, 1078, 1246
HgaI GACGC 3 cut(s) 54, 373, 555
HhaI GCGC 2 cut(s) 999, 1231
Hin1II CATG 8 cut(s) 148, 430, 481, 538, 874, 913, 1123, 1213
Hin6I GCGC 2 cut(s) 997, 1229
HinP1I GCGC 2 cut(s) 997, 1229
HincII GTYRAC 2 cut(s) 157, 181
HindII GTYRAC 2 cut(s) 157, 181
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 7 cut(s) 539, 558, 585, 604, 836, 1094, 1100
HpaII CCGG 6 cut(s) 152, 349, 1004, 1046, 1078, 1246
HphI GGTGA 4 cut(s) 179, 432, 613, 653
Hpy166II GTNNAC 4 cut(s) 157, 181, 262, 757
Hpy188I TCNGA 8 cut(s) 115, 458, 720, 725, 783, 1068, 1089, 1099
Hpy8I GTNNAC 4 cut(s) 157, 181, 262, 757
HpyAV CCTTC 5 cut(s) 149, 239, 778, 1084, 1133
HpyCH4III ACNGT 2 cut(s) 24, 380
HpyCH4IV ACGT 1 cut(s) 825
HpyCH4V TGCA 4 cut(s) 148, 411, 809, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 334, 815
HpyF3I CTNAG 3 cut(s) 760, 984, 1086
HpySE526I ACGT 1 cut(s) 825
Hsp92II CATG 8 cut(s) 148, 430, 481, 538, 874, 913, 1123, 1213
HspAI GCGC 2 cut(s) 997, 1229
Kpn2I TCCGGA 1 cut(s) 1077
Ksp22I TGATCA 1 cut(s) 720
Kzo9I GATC 4 cut(s) 129, 231, 720, 873
LmnI GCTCC 1 cut(s) 815
Lsp1109I GCAGC 3 cut(s) 73, 379, 423
LweI GCATC 3 cut(s) 157, 398, 507
MaeI CTAG 4 cut(s) 623, 710, 902, 1215
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 6 cut(s) 289, 601, 686, 950, 979, 1007
MalI GATC 4 cut(s) 131, 233, 722, 875
MboI GATC 4 cut(s) 129, 231, 720, 873
MboII GAAGA 7 cut(s) 239, 311, 463, 481, 676, 868, 1208
MfeI CAATTG 1 cut(s) 810
MhlI GDGCHC 1 cut(s) 820
MlyI GAGTC 3 cut(s) 598, 845, 1109
Mph1103I ATGCAT 1 cut(s) 150
MroI TCCGGA 1 cut(s) 1077
MroXI GAANNNNTTC 2 cut(s) 452, 788
MseI TTAA 1 cut(s) 933
MspI CCGG 6 cut(s) 152, 349, 1004, 1046, 1078, 1246
MspR9I CCNGG 5 cut(s) 191, 639, 684, 976, 1005
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 2 cut(s) 414, 811
MvaI CCWGG 4 cut(s) 191, 639, 684, 976
MvnI CGCG 2 cut(s) 549, 599
MwoI GCNNNNNNNGC 2 cut(s) 334, 815
NciI CCSGG 1 cut(s) 1005
NcoI CCATGG 1 cut(s) 477
NdeII GATC 4 cut(s) 129, 231, 720, 873
NlaIII CATG 8 cut(s) 148, 430, 481, 538, 874, 913, 1123, 1213
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 601, 950
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 1 cut(s) 1123
PagI TCATGA 1 cut(s) 870
PcsI WCGNNNNNNNCGW 1 cut(s) 553
PctI GAATGC 2 cut(s) 414, 811
PdmI GAANNNNTTC 2 cut(s) 452, 788
PfeI GAWTC 4 cut(s) 539, 558, 585, 1094
PflMI CCANNNNNTGG 1 cut(s) 846
PkrI GCNGC 3 cut(s) 88, 369, 413
PleI GAGTC 3 cut(s) 598, 844, 1108
PpsI GAGTC 3 cut(s) 598, 844, 1108
PsiI TTATAA 1 cut(s) 33
Psp6I CCWGG 4 cut(s) 189, 637, 682, 974
PspGI CCWGG 4 cut(s) 189, 637, 682, 974
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 4 cut(s) 248, 351, 849, 959
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 3 cut(s) 87, 368, 412
Sau3AI GATC 4 cut(s) 129, 231, 720, 873
Sau96I GGNCC 4 cut(s) 248, 351, 849, 959
SchI GAGTC 3 cut(s) 598, 845, 1109
ScrFI CCNGG 5 cut(s) 191, 639, 684, 976, 1005
SduI GDGCHC 1 cut(s) 820
SfaNI GCATC 3 cut(s) 157, 398, 507
SinI GGWCC 4 cut(s) 248, 351, 849, 959
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
SsiI CCGC 4 cut(s) 322, 549, 599, 893
SspMI CTAG 4 cut(s) 623, 710, 902, 1215
StyD4I CCNGG 5 cut(s) 189, 637, 682, 974, 1003
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 2 cut(s) 24, 380
TaiI ACGT 1 cut(s) 828
TaqI TCGA 5 cut(s) 128, 382, 527, 879, 1103
TfiI GAWTC 4 cut(s) 539, 558, 585, 1094
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 2 cut(s) 601, 950
TseI GCWGC 3 cut(s) 86, 367, 411
Tsp45I GTSAC 2 cut(s) 601, 950
TspGWI ACGGA 2 cut(s) 420, 972
TspRI CASTG 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 846
VpaK11BI GGWCC 4 cut(s) 248, 351, 849, 959
XapI RAATTY 4 cut(s) 467, 645, 917, 1062
XceI RCATGY 1 cut(s) 1123
XcmI CCANNNNNNNNNTGG 1 cut(s) 645
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 2 cut(s) 452, 788
XspI CTAG 4 cut(s) 623, 710, 902, 1215
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.