FvH4_6g12350

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011163
Physical Location & Seq
Reverse (-)
7468453 .. 7472374
3922 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12350.t1

Sequence Viewer

Length: 993 bp
ATGACCCAAGGACTTCTCCAATGGAGTCCTTGCCACCACTCCCTCTCCCTCCTCCCCATCTCCATCCCCACATCCAAACACACCCACCTCCCTCTCTCCATCTCCGCATCTCTAGAATCCTCCACCCAACAACAACCACAGCTGTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGCGAGAGAGAAAAGCAGGCACCAGCTGGAAAGAAAAGGTCGAGGAGAAGCTTCTGGAAAAGCCCACCCAGAAATTCGCGCACTGGAAAGAAGAGCTCAACATTAACAACCTCGCTCGCGAGGGCCCACAGTGGTGGATCGTCCGAGTTTCTAGGATTAAGGGACAGGAGACAGCTCAGTTAATTGCTCGGTTGCTCGCTAGGAACTACCCTCATATGGATTTCAAGGTATATGCGCCGTCGATACCGGAGAGGAGGAAATTGAAGAATGGTACATACTCGGTTAAAGCAAAGCCACTGTTTCCGGGATGTGTCTTTTTGAGGTGTGTGTTGGACAAGGAGATACATGATTTCGTGACGGAGTTGGATGGAGTTGGAGGCTTTATTGGTGCCAAGGTTGGAAATACGAAGAGACAGATTAACAGACCCAGGCCAGTGTCTGAATTTGACATGGAAGCTATCTTCGCAAAGGCAAAGGAAGAACAAGAAAAATCTGACCTAGCTTTTCAACAAGAGCAGCAACTCAGCTCTGAGCTGAAATCTAGTGATGCTGCAAAATCTGCTGGAGATGCTAAACCCAAAAGGAGATCCGGAAAAGCTTCTGATCCTCTGATAAATGGTTCATCTACGGCAAAGGATAAAAAACTCGTTCTAGGTTCTAAAGTTCGAGTTCTATCTGGGACTTTTGCTGAATATGAAGGCAGCCTGAAGAAGCTGAATCGCAGAACCAAAAAGGCGACCGTGGCATTTATGCTCTTTGGGAAAGAAAGCTTAGTAGATTTAGATGTCAGTGAAATTGTTTCAGAGACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

331

Amino Acids

37.22

Weight (kDa)

9.78

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusG PF02357 103 - 211 3.2e-07 Transcription termination factor nusG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 197, 566
AccII CGCG 2 cut(s) 257, 297
AccIII TCCGGA 1 cut(s) 767
AciI CCGC 2 cut(s) 105, 147
AclWI GGATC 3 cut(s) 323, 759, 776
AcsI RAATTY 2 cut(s) 251, 620
AcuI CTGAAG 1 cut(s) 905
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 3 cut(s) 403, 442, 686
AjnI CCWGG 1 cut(s) 605
AleI CACNNNNGTG 1 cut(s) 310
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 3 cut(s) 341, 583, 977
AlwI GGATC 3 cut(s) 323, 759, 776
AlwNI CAGNNNCTG 1 cut(s) 617
Aor13HI TCCGGA 1 cut(s) 767
AoxI GGCC 2 cut(s) 301, 608
ApaI GGGCCC 1 cut(s) 305
ApeKI GCWGC 4 cut(s) 163, 694, 728, 879
ApoI RAATTY 2 cut(s) 251, 620
Asp700I GAANNNNTTC 1 cut(s) 775
AspLEI GCGC 2 cut(s) 259, 415
AspS9I GGNCC 2 cut(s) 301, 302
AsuC2I CCSGG 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 305
BanI GGYRCC 2 cut(s) 197, 566
BanII GRGCYC 2 cut(s) 276, 305
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 4 cut(s) 175, 706, 715, 891
BccI CCATC 4 cut(s) 65, 71, 107, 539
BceAI ACGGC 2 cut(s) 400, 822
BciT130I CCWGG 1 cut(s) 607
BcnI CCSGG 1 cut(s) 483
BcoDI GTCTC 3 cut(s) 341, 583, 977
BfaI CTAG 6 cut(s) 113, 330, 378, 677, 720, 830
BisI GCNGC 4 cut(s) 164, 695, 729, 880
BlsI GCNGC 4 cut(s) 165, 696, 730, 881
Bme1390I CCNGG 2 cut(s) 483, 607
BmgT120I GGNCC 2 cut(s) 301, 302
BmiI GGNNCC 3 cut(s) 199, 303, 568
BmrFI CCNGG 2 cut(s) 483, 607
BmsI GCATC 3 cut(s) 116, 715, 736
BpmI CTGGAG 1 cut(s) 762
BpuMI CCSGG 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 4 cut(s) 7, 570, 605, 918
BsaWI WCCGGW 2 cut(s) 424, 767
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 266, 611
BseAI TCCGGA 1 cut(s) 767
BseBI CCWGG 1 cut(s) 607
BseDI CCNNGG 4 cut(s) 7, 570, 605, 918
BseGI GGATG 4 cut(s) 63, 71, 491, 550
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 4 cut(s) 181, 368, 699, 715
BseNI ACTGG 2 cut(s) 266, 611
BseRI GAGGAG 3 cut(s) 41, 236, 445
BseSI GKGCMC 1 cut(s) 305
BseXI GCAGC 4 cut(s) 175, 706, 715, 891
Bsh1236I CGCG 2 cut(s) 257, 297
Bsh1285I CGRYCG 1 cut(s) 918
BshFI GGCC 2 cut(s) 303, 610
BshNI GGYRCC 2 cut(s) 197, 566
BsiEI CGRYCG 1 cut(s) 918
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 3 cut(s) 425, 482, 768
BslFI GGGAC 2 cut(s) 354, 871
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 3 cut(s) 341, 583, 977
BsmFI GGGAC 2 cut(s) 354, 871
BsnI GGCC 2 cut(s) 303, 610
Bso31I GGTCTC 1 cut(s) 977
Bsp120I GGGCCC 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 276, 305
Bsp13I TCCGGA 1 cut(s) 767
Bsp143I GATC 3 cut(s) 315, 764, 781
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 2 cut(s) 105, 147
BspANI GGCC 2 cut(s) 303, 610
BspCNI CTCAG 4 cut(s) 180, 367, 700, 714
BspEI TCCGGA 1 cut(s) 767
BspFNI CGCG 2 cut(s) 257, 297
BspLI GGNNCC 3 cut(s) 199, 303, 568
BspPI GGATC 3 cut(s) 323, 759, 776
BspQI GCTCTTC 1 cut(s) 264
BspT107I GGYRCC 2 cut(s) 197, 566
BspTNI GGTCTC 1 cut(s) 977
BsrI ACTGG 2 cut(s) 266, 611
BssECI CCNNGG 4 cut(s) 7, 570, 605, 918
BssMI GATC 3 cut(s) 315, 764, 781
BssT1I CCWWGG 2 cut(s) 7, 570
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 3 cut(s) 309, 477, 919
Bst6I CTCTTC 2 cut(s) 264, 581
BstAPI GCANNNNNTGC 1 cut(s) 737
BstC8I GCNNGC 3 cut(s) 196, 295, 375
BstDEI CTNAG 5 cut(s) 167, 354, 701, 708, 949
BstDSI CCRYGG 1 cut(s) 918
BstF5I GGATG 4 cut(s) 63, 71, 491, 550
BstFNI CGCG 2 cut(s) 257, 297
BstHHI GCGC 2 cut(s) 259, 415
BstKTI GATC 3 cut(s) 318, 767, 784
BstMAI GTCTC 3 cut(s) 341, 583, 977
BstMBI GATC 3 cut(s) 315, 764, 781
BstMCI CGRYCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 4 cut(s) 641, 737, 746, 920
BstNI CCWGG 1 cut(s) 607
BstSCI CCNGG 2 cut(s) 481, 605
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 2 cut(s) 257, 297
BstV1I GCAGC 4 cut(s) 175, 706, 715, 891
BstX2I RGATCY 1 cut(s) 764
BstXI CCANNNNNNTGG 1 cut(s) 312
BstYI RGATCY 1 cut(s) 764
BsuRI GGCC 2 cut(s) 303, 610
BtgI CCRYGG 1 cut(s) 918
BtsCI GGATG 4 cut(s) 63, 71, 491, 550
BtsIMutI CAGTG 5 cut(s) 259, 314, 473, 618, 973
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 196, 295, 375
CaiI CAGNNNCTG 1 cut(s) 617
CfoI GCGC 2 cut(s) 259, 415
Cfr13I GGNCC 2 cut(s) 301, 302
Csp6I GTAC 1 cut(s) 450
CviAII CATG 2 cut(s) 524, 628
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 5 cut(s) 167, 354, 701, 708, 949
DpnI GATC 3 cut(s) 317, 766, 783
DpnII GATC 3 cut(s) 315, 764, 781
Eam1104I CTCTTC 2 cut(s) 264, 581
EarI CTCTTC 2 cut(s) 264, 581
EciI GGCGGA 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 2 cut(s) 7, 570
Eco24I GRGCYC 2 cut(s) 276, 305
Eco31I GGTCTC 1 cut(s) 977
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 905
EcoICRI GAGCTC 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 605
EcoT14I CCWWGG 2 cut(s) 7, 570
EcoT38I GRGCYC 2 cut(s) 276, 305
ErhI CCWWGG 2 cut(s) 7, 570
FaeI CATG 2 cut(s) 527, 631
FaiI YATR 9 cut(s) 393, 395, 409, 411, 454, 525, 629, 873, 929
FaqI GGGAC 2 cut(s) 354, 871
FatI CATG 2 cut(s) 523, 627
FauNDI CATATG 1 cut(s) 393
Fnu4HI GCNGC 4 cut(s) 164, 695, 729, 880
FokI GGATG 4 cut(s) 50, 58, 498, 557
FriOI GRGCYC 2 cut(s) 276, 305
Fsp4HI GCNGC 4 cut(s) 164, 695, 729, 880
FspBI CTAG 6 cut(s) 113, 330, 378, 677, 720, 830
GlaI GCGC 2 cut(s) 258, 414
GluI GCNGC 4 cut(s) 164, 695, 729, 880
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 2 cut(s) 303, 610
HapII CCGG 3 cut(s) 425, 482, 768
HhaI GCGC 2 cut(s) 259, 415
Hin1II CATG 2 cut(s) 527, 631
Hin6I GCGC 2 cut(s) 257, 413
HinP1I GCGC 2 cut(s) 257, 413
HindIII AAGCTT 3 cut(s) 227, 774, 946
HinfI GANTC 3 cut(s) 25, 116, 895
HpaII CCGG 3 cut(s) 425, 482, 768
Hpy188I TCNGA 8 cut(s) 170, 323, 619, 673, 709, 781, 789, 982
Hpy188III TCNNGA 5 cut(s) 113, 233, 296, 532, 768
Hpy99I CGWCG 1 cut(s) 421
HpyAV CCTTC 2 cut(s) 153, 869
HpyCH4III ACNGT 3 cut(s) 309, 477, 919
HpyCH4V TGCA 1 cut(s) 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 641, 737, 746, 920
HpyF3I CTNAG 5 cut(s) 167, 354, 701, 708, 949
Hsp92II CATG 2 cut(s) 527, 631
HspAI GCGC 2 cut(s) 257, 413
Kpn2I TCCGGA 1 cut(s) 767
Kzo9I GATC 3 cut(s) 315, 764, 781
LguI GCTCTTC 1 cut(s) 264
Lsp1109I GCAGC 4 cut(s) 175, 706, 715, 891
LweI GCATC 3 cut(s) 116, 715, 736
MaeI CTAG 6 cut(s) 113, 330, 378, 677, 720, 830
MaeIII GTNAC 1 cut(s) 532
MalI GATC 3 cut(s) 317, 766, 783
MboI GATC 3 cut(s) 315, 764, 781
MboII GAAGA 6 cut(s) 281, 454, 598, 631, 668, 898
MflI RGATCY 1 cut(s) 764
MhlI GDGCHC 2 cut(s) 276, 305
MluCI AATT 5 cut(s) 251, 360, 437, 620, 972
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 4 cut(s) 489, 522, 532, 556
MroI TCCGGA 1 cut(s) 767
MroXI GAANNNNTTC 1 cut(s) 775
MseI TTAA 5 cut(s) 282, 336, 359, 462, 597
MslI CAYNNNNRTG 1 cut(s) 310
MspA1I CMGCKG 2 cut(s) 142, 204
MspI CCGG 3 cut(s) 425, 482, 768
MspR9I CCNGG 2 cut(s) 483, 607
MvaI CCWGG 1 cut(s) 607
MvnI CGCG 2 cut(s) 257, 297
MwoI GCNNNNNNNGC 4 cut(s) 641, 737, 746, 920
NciI CCSGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 393
NdeII GATC 3 cut(s) 315, 764, 781
NlaIII CATG 2 cut(s) 527, 631
NlaIV GGNNCC 3 cut(s) 199, 303, 568
NmuCI GTSAC 1 cut(s) 532
NruI TCGCGA 1 cut(s) 297
OliI CACNNNNGTG 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 775
PfeI GAWTC 2 cut(s) 116, 895
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 4 cut(s) 165, 696, 730, 881
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 605
PspGI CCWGG 1 cut(s) 605
PspN4I GGNNCC 3 cut(s) 199, 303, 568
PspOMI GGGCCC 1 cut(s) 301
PspPI GGNCC 2 cut(s) 301, 302
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 1 cut(s) 764
PvuII CAGCTG 2 cut(s) 142, 204
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 310
SacI GAGCTC 1 cut(s) 276
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 5 cut(s) 282, 336, 359, 462, 597
SatI GCNGC 4 cut(s) 164, 695, 729, 880
Sau3AI GATC 3 cut(s) 315, 764, 781
Sau96I GGNCC 2 cut(s) 301, 302
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 483, 607
SduI GDGCHC 2 cut(s) 276, 305
SfaNI GCATC 3 cut(s) 116, 715, 736
SmiMI CAYNNNNRTG 1 cut(s) 310
Sse9I AATT 5 cut(s) 251, 360, 437, 620, 972
SsiI CCGC 2 cut(s) 105, 147
SspMI CTAG 6 cut(s) 113, 330, 378, 677, 720, 830
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 481, 605
StyI CCWWGG 2 cut(s) 7, 570
TaaI ACNGT 3 cut(s) 309, 477, 919
TaqI TCGA 3 cut(s) 219, 419, 844
TasI AATT 5 cut(s) 251, 360, 437, 620, 972
TfiI GAWTC 2 cut(s) 116, 895
Tru1I TTAA 5 cut(s) 282, 336, 359, 462, 597
Tru9I TTAA 5 cut(s) 282, 336, 359, 462, 597
TscAI CASTG 5 cut(s) 266, 314, 480, 618, 973
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 4 cut(s) 163, 694, 728, 879
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 2 cut(s) 789, 888
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 5 cut(s) 266, 314, 480, 618, 973
XapI RAATTY 2 cut(s) 251, 620
XbaI TCTAGA 1 cut(s) 112
XmnI GAANNNNTTC 1 cut(s) 775
XspI CTAG 6 cut(s) 113, 330, 378, 677, 720, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.