Rw3G025130

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011163
Physical Location & Seq
Reverse (-)
33041661 .. 33042258
598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025130.1

Sequence Viewer

Length: 498 bp
ATGACCCAAGAACTTCTCCAATGGAGTCCTTGCCACCACTCTCTCTCCCTCCTCCCCATCTCCATCCCCACATCCAAACACACCCACCTCCCTCTCTCCATCTCCGCCACTCTAGAATCCTCCACCCAACAACAGCAGCAGCTCTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGAGAGAGAGCAAAGCCGGCACCAACTGGAAAGAAAAGGTCGAGGAGAAGCTTCTGGAGAAGCCCACCCAGAAATTCGTTCACTGGAAAGAAGAGCTCAACATCAACAACCTCGCTCGCGAGGGCCCACAGTGGTGGATCGTTCGAGTTTCTAGAGTCAAGGGCCAGGACACGGCTCAGTTAATCGCTCGGTTGCTCGCCAGGAACTACCCACATATGGATTTGAAGGTTTATGCTCCAACGATACCGGAGAGGAGGAAACTGAAGAATGGTACCTACTCGGTTAAAGCAAAGCGCTGTTTCCGGGGTGTGCTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

165

Amino Acids

19.34

Weight (kDa)

10.25

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 449
AccB1I GGYRCC 2 cut(s) 197, 449
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 105, 147
AclWI GGATC 1 cut(s) 323
AcsI RAATTY 1 cut(s) 251
AcuI CTGAAG 1 cut(s) 461
AfaI GTAC 1 cut(s) 451
AfeI AGCGCT 1 cut(s) 473
AfiI CCNNNNNNNGG 2 cut(s) 349, 394
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 2 cut(s) 342, 377
AleI CACNNNNGTG 1 cut(s) 310
AluBI AGCT 4 cut(s) 142, 166, 229, 274
AluI AGCT 4 cut(s) 142, 166, 229, 274
Alw21I GWGCWC 1 cut(s) 276
AlwI GGATC 1 cut(s) 323
Aor51HI AGCGCT 1 cut(s) 473
AoxI GGCC 2 cut(s) 301, 340
ApaI GGGCCC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 136, 139, 163
ApoI RAATTY 1 cut(s) 251
Asp718I GGTACC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 474
AspS9I GGNCC 3 cut(s) 301, 302, 340
AsuC2I CCSGG 1 cut(s) 482
BaeGI GKGCMC 1 cut(s) 305
BanI GGYRCC 2 cut(s) 197, 449
BanII GRGCYC 2 cut(s) 276, 305
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 3 cut(s) 148, 151, 175
BccI CCATC 3 cut(s) 65, 71, 107
BceAI ACGGC 1 cut(s) 366
BciT130I CCWGG 2 cut(s) 344, 379
BcnI CCSGG 1 cut(s) 482
BfaI CTAG 2 cut(s) 113, 330
BfoI RGCGCY 1 cut(s) 475
BisI GCNGC 3 cut(s) 137, 140, 164
BlsI GCNGC 3 cut(s) 138, 141, 165
Bme1390I CCNGG 3 cut(s) 344, 379, 482
BmgT120I GGNCC 3 cut(s) 301, 302, 340
BmiI GGNNCC 3 cut(s) 199, 303, 451
BmrFI CCNGG 3 cut(s) 344, 379, 482
BpmI CTGGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 481
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 394
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 209, 266
BseBI CCWGG 2 cut(s) 344, 379
BseDI CCNNGG 1 cut(s) 481
BseGI GGATG 2 cut(s) 63, 71
BseLI CCNNNNNNNGG 2 cut(s) 349, 394
BseMII CTCAG 2 cut(s) 181, 368
BseNI ACTGG 2 cut(s) 209, 266
BseRI GAGGAG 4 cut(s) 41, 193, 236, 445
BseSI GKGCMC 1 cut(s) 305
BseXI GCAGC 3 cut(s) 148, 151, 175
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 2 cut(s) 303, 342
BshNI GGYRCC 2 cut(s) 197, 449
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 3 cut(s) 195, 425, 481
BslI CCNNNNNNNGG 2 cut(s) 349, 394
BsnI GGCC 2 cut(s) 303, 342
Bsp120I GGGCCC 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 276, 305
Bsp143I GATC 1 cut(s) 315
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 2 cut(s) 105, 147
BspANI GGCC 2 cut(s) 303, 342
BspCNI CTCAG 2 cut(s) 180, 367
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 199, 303, 451
BspPI GGATC 1 cut(s) 323
BspQI GCTCTTC 1 cut(s) 264
BspT107I GGYRCC 2 cut(s) 197, 449
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 2 cut(s) 209, 266
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 344, 379
Bst4CI ACNGT 1 cut(s) 309
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 196, 295, 375
BstDEI CTNAG 2 cut(s) 167, 354
BstF5I GGATG 2 cut(s) 63, 71
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 475
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 1 cut(s) 318
BstMBI GATC 1 cut(s) 315
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 2 cut(s) 344, 379
BstSCI CCNGG 3 cut(s) 342, 377, 480
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 148, 151, 175
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 2 cut(s) 303, 342
BtsCI GGATG 2 cut(s) 63, 71
BtsIMutI CAGTG 2 cut(s) 259, 314
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 3 cut(s) 196, 295, 375
CfoI GCGC 1 cut(s) 474
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 301, 302, 340
Csp6I GTAC 1 cut(s) 450
CviJI RGCY 9 cut(s) 142, 166, 194, 229, 241, 274, 303, 342, 353
CviKI_1 RGCY 9 cut(s) 142, 166, 194, 229, 241, 274, 303, 342, 353
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 167, 354
DpnI GATC 1 cut(s) 317
DpnII GATC 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EciI GGCGGA 2 cut(s) 94, 136
Ecl136II GAGCTC 1 cut(s) 274
Eco24I GRGCYC 2 cut(s) 276, 305
Eco47III AGCGCT 1 cut(s) 473
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 274
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 342, 377
EcoT38I GRGCYC 2 cut(s) 276, 305
FaiI YATR 3 cut(s) 393, 395, 411
FauNDI CATATG 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 137, 140, 164
FokI GGATG 2 cut(s) 50, 58
FriOI GRGCYC 2 cut(s) 276, 305
Fsp4HI GCNGC 3 cut(s) 137, 140, 164
FspBI CTAG 2 cut(s) 113, 330
GlaI GCGC 1 cut(s) 473
GluI GCNGC 3 cut(s) 137, 140, 164
GsuI CTGGAG 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 475
HaeIII GGCC 2 cut(s) 303, 342
HapII CCGG 3 cut(s) 195, 425, 481
HhaI GCGC 1 cut(s) 474
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 3 cut(s) 25, 116, 333
HpaII CCGG 3 cut(s) 195, 425, 481
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 4 cut(s) 113, 233, 296, 330
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 153, 397
HpyCH4III ACNGT 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 2 cut(s) 167, 354
HspAI GCGC 1 cut(s) 472
KpnI GGTACC 1 cut(s) 453
KroI GCCGGC 1 cut(s) 194
KroNI GCCGGC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 315
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 3 cut(s) 148, 151, 175
MaeI CTAG 2 cut(s) 113, 330
MalI GATC 1 cut(s) 317
MboI GATC 1 cut(s) 315
MboII GAAGA 2 cut(s) 281, 454
MhlI GDGCHC 2 cut(s) 276, 305
MluCI AATT 1 cut(s) 251
MlyI GAGTC 2 cut(s) 34, 342
MmeI TCCRAC 1 cut(s) 440
MroNI GCCGGC 1 cut(s) 194
MseI TTAA 2 cut(s) 359, 462
MslI CAYNNNNRTG 1 cut(s) 310
MspI CCGG 3 cut(s) 195, 425, 481
MspR9I CCNGG 3 cut(s) 344, 379, 482
MvaI CCWGG 2 cut(s) 344, 379
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 195
NaeI GCCGGC 1 cut(s) 196
NciI CCSGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 393
NdeII GATC 1 cut(s) 315
NgoMIV GCCGGC 1 cut(s) 194
NlaIV GGNNCC 3 cut(s) 199, 303, 451
NruI TCGCGA 1 cut(s) 297
OliI CACNNNNGTG 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 3 cut(s) 138, 141, 165
PleI GAGTC 2 cut(s) 33, 341
PpsI GAGTC 2 cut(s) 33, 341
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 342, 377
PspGI CCWGG 2 cut(s) 342, 377
PspN4I GGNNCC 3 cut(s) 199, 303, 451
PspOMI GGGCCC 1 cut(s) 301
PspPI GGNCC 3 cut(s) 301, 302, 340
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 310
SacI GAGCTC 1 cut(s) 276
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 359, 462
SatI GCNGC 3 cut(s) 137, 140, 164
Sau3AI GATC 1 cut(s) 315
Sau96I GGNCC 3 cut(s) 301, 302, 340
SchI GAGTC 2 cut(s) 34, 342
ScrFI CCNGG 3 cut(s) 344, 379, 482
SduI GDGCHC 2 cut(s) 276, 305
SetI ASST 9 cut(s) 90, 144, 168, 219, 231, 276, 291, 408, 455
SmiMI CAYNNNNRTG 1 cut(s) 310
Sse9I AATT 1 cut(s) 251
SsiI CCGC 2 cut(s) 105, 147
SspMI CTAG 2 cut(s) 113, 330
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 3 cut(s) 342, 377, 480
TaaI ACNGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 219, 322
TasI AATT 1 cut(s) 251
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 2 cut(s) 359, 462
Tru9I TTAA 2 cut(s) 359, 462
TscAI CASTG 2 cut(s) 266, 314
TseI GCWGC 3 cut(s) 136, 139, 163
TspRI CASTG 2 cut(s) 266, 314
XapI RAATTY 1 cut(s) 251
XbaI TCTAGA 2 cut(s) 112, 329
XspI CTAG 2 cut(s) 113, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.