RLG00000024718

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011163
Physical Location & Seq
Forward (+)
38522111 .. 38523915
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024718

Sequence Viewer

Length: 1017 bp
ATGACCCAAGGACTTCTCCAATGGAGTCCTTGCCACCACTCTCTCTCCCTCCTCACCATAACCATCCCCACAATCAAACACACCCACCTCCCTCTCTCAATCTCCGCCACTCTAGAATCCTCCACCACCCAACAACAGCAGCAGCAGCAGCTCTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGAGAGAGAGCAAAGCCGGCACCAACTGGAAAGAACAGGTCGAGGAGAAGCTTCTGGAGAAGCCCACCCAGAAATTCGCTCACTGGAAAGAAGAGCTCAACATCAACAACCTCGCTCGCGAGGGCCCGCAGTGGTGGATCGTTCGAGTTTCTAGGGTCAAGGGCCAGGAGACGGCTCAGTTAATCGCTCGGTTGCTCGCTAGGAACTACCCACATATGGATTTCAAGGTTTATGCTCCAACGATACCAGAGAGGAGGAAATTGAAGAATGGTACCTACTCGGTTAAAGCAAAGCCACTGTTTCCAGGGTGTGTTTTTTTGAGGTGTGTGTTGGACAAGGAGATACATGATTTCGTGACGGAGTTGGATGGAGTTGGAGGCTTTATTGGTGCCAAGGTTGGAAATACGAAGAGACAGATCAACAGACCCAGGCCAGTATCTGAGTTTGACATGGAAGCTATCTTCGCAAAGGCAAAGGAAGAACAAGAAAAAGCTGAACTAGCTTTTCAACAAGAGCAGCAACTCAACTCTGGGCTGAATATTGATCATCTGAATTCTAGTGATGCTGCAAAATCTGCTGGAGATGCTAAATCCAAAGAGAGATCCAGAAAAGCTGCTGATACTCTGGTAAATGGTTCATCTAAGGCAAAGGATAAGAAACTTGTCGTAGGTTCTACTGTTCGAGTTGTATCTGGGACTTTTGCTGAATATGAAGGCTGCCTGAAGAAGCTGAATCGCAGAACCAAAAAGGCAACCGTGGCATTTACGTTATTTGGGAAAGAGAGCTTAGTAGATTTAGATGTCAGTGAAATTGTTTCAGAGACCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

339

Amino Acids

38.15

Weight (kDa)

9.57

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusG PF02357 106 - 214 8.8e-08 Transcription termination factor nusG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1015
Acc65I GGTACC 1 cut(s) 458
AccB1I GGYRCC 3 cut(s) 206, 458, 575
AccII CGCG 1 cut(s) 306
AciI CCGC 3 cut(s) 105, 156, 314
AclWI GGATC 2 cut(s) 332, 783
AcsI RAATTY 2 cut(s) 260, 739
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 2 cut(s) 358, 403
AgsI TTSAA 3 cut(s) 412, 451, 695
AjnI CCWGG 3 cut(s) 351, 490, 614
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 3 cut(s) 350, 592, 1001
AlwI GGATC 2 cut(s) 332, 783
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 3 cut(s) 310, 349, 617
ApaI GGGCCC 1 cut(s) 314
ApeKI GCWGC 9 cut(s) 139, 142, 145, 148, 172, 703, 752, 800, 903
ApoI RAATTY 2 cut(s) 260, 739
Asp718I GGTACC 1 cut(s) 458
AspS9I GGNCC 3 cut(s) 310, 311, 349
AsuHPI GGTGA 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 314
BaeI ACNNNNGTAYC 2 cut(s) 798, 831
BanI GGYRCC 3 cut(s) 206, 458, 575
BanII GRGCYC 2 cut(s) 285, 314
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 9 cut(s) 151, 154, 157, 160, 184, 715, 739, 787, 890
BccI CCATC 2 cut(s) 71, 548
BceAI ACGGC 1 cut(s) 375
BciT130I CCWGG 3 cut(s) 353, 492, 616
BclI TGATCA 1 cut(s) 730
BcoDI GTCTC 3 cut(s) 350, 592, 1001
BfaI CTAG 5 cut(s) 113, 339, 387, 686, 744
BisI GCNGC 9 cut(s) 140, 143, 146, 149, 173, 704, 753, 801, 904
BlsI GCNGC 9 cut(s) 141, 144, 147, 150, 174, 705, 754, 802, 905
Bme1390I CCNGG 3 cut(s) 353, 492, 616
BmgT120I GGNCC 3 cut(s) 310, 311, 349
BmiI GGNNCC 4 cut(s) 208, 312, 460, 577
BmrFI CCNGG 3 cut(s) 353, 492, 616
BmsI GCATC 2 cut(s) 739, 760
BpmI CTGGAG 2 cut(s) 263, 786
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 5 cut(s) 7, 491, 579, 614, 942
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 403
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 3 cut(s) 218, 275, 620
BseBI CCWGG 3 cut(s) 353, 492, 616
BseDI CCNNGG 5 cut(s) 7, 491, 579, 614, 942
BseGI GGATG 2 cut(s) 63, 559
BseLI CCNNNNNNNGG 2 cut(s) 358, 403
BseMII CTCAG 3 cut(s) 190, 377, 618
BseNI ACTGG 3 cut(s) 218, 275, 620
BseRI GAGGAG 4 cut(s) 41, 202, 245, 454
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 9 cut(s) 151, 154, 157, 160, 184, 715, 739, 787, 890
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 3 cut(s) 312, 351, 619
BshNI GGYRCC 3 cut(s) 206, 458, 575
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 895
BslI CCNNNNNNNGG 2 cut(s) 358, 403
BsmAI GTCTC 3 cut(s) 350, 592, 1001
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 895
BsnI GGCC 3 cut(s) 312, 351, 619
Bso31I GGTCTC 1 cut(s) 1001
Bsp120I GGGCCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 285, 314
Bsp143I GATC 4 cut(s) 324, 603, 730, 788
Bsp68I TCGCGA 1 cut(s) 306
BspACI CCGC 3 cut(s) 105, 156, 314
BspANI GGCC 3 cut(s) 312, 351, 619
BspCNI CTCAG 3 cut(s) 189, 376, 619
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 4 cut(s) 208, 312, 460, 577
BspPI GGATC 2 cut(s) 332, 783
BspQI GCTCTTC 1 cut(s) 273
BspT107I GGYRCC 3 cut(s) 206, 458, 575
BspTNI GGTCTC 1 cut(s) 1001
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 3 cut(s) 218, 275, 620
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 5 cut(s) 7, 491, 579, 614, 942
BssMI GATC 4 cut(s) 324, 603, 730, 788
BssT1I CCWWGG 2 cut(s) 7, 579
Bst2UI CCWGG 3 cut(s) 353, 492, 616
Bst4CI ACNGT 3 cut(s) 486, 865, 943
Bst6I CTCTTC 2 cut(s) 273, 590
BstAPI GCANNNNNTGC 1 cut(s) 761
BstC8I GCNNGC 4 cut(s) 205, 304, 314, 384
BstDEI CTNAG 5 cut(s) 176, 363, 627, 828, 973
BstDSI CCRYGG 1 cut(s) 942
BstF5I GGATG 2 cut(s) 63, 559
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 4 cut(s) 327, 606, 733, 791
BstMAI GTCTC 3 cut(s) 350, 592, 1001
BstMBI GATC 4 cut(s) 324, 603, 730, 788
BstMWI GCNNNNNNNGC 8 cut(s) 145, 148, 204, 650, 686, 761, 770, 944
BstNI CCWGG 3 cut(s) 353, 492, 616
BstSCI CCNGG 3 cut(s) 351, 490, 614
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 9 cut(s) 151, 154, 157, 160, 184, 715, 739, 787, 890
BstX2I RGATCY 1 cut(s) 788
BstYI RGATCY 1 cut(s) 788
BsuRI GGCC 3 cut(s) 312, 351, 619
BtgI CCRYGG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 63, 559
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 4 cut(s) 268, 323, 482, 997
BtuMI TCGCGA 1 cut(s) 306
Cac8I GCNNGC 4 cut(s) 205, 304, 314, 384
CaiI CAGNNNCTG 1 cut(s) 626
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 3 cut(s) 310, 311, 349
Csp6I GTAC 1 cut(s) 459
CviAII CATG 2 cut(s) 533, 637
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 5 cut(s) 176, 363, 627, 828, 973
DpnI GATC 4 cut(s) 326, 605, 732, 790
DpnII GATC 4 cut(s) 324, 603, 730, 788
Eam1104I CTCTTC 2 cut(s) 273, 590
EarI CTCTTC 2 cut(s) 273, 590
EciI GGCGGA 2 cut(s) 94, 145
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 7, 579
Eco24I GRGCYC 2 cut(s) 285, 314
Eco31I GGTCTC 1 cut(s) 1001
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 929
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 739
EcoRII CCWGG 3 cut(s) 351, 490, 614
EcoT14I CCWWGG 2 cut(s) 7, 579
EcoT38I GRGCYC 2 cut(s) 285, 314
ErhI CCWWGG 2 cut(s) 7, 579
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 2 cut(s) 536, 640
FaiI YATR 8 cut(s) 59, 402, 404, 420, 534, 638, 897, 1015
FaqI GGGAC 1 cut(s) 895
FatI CATG 2 cut(s) 532, 636
FauI CCCGC 1 cut(s) 321
FauNDI CATATG 1 cut(s) 402
FbaI TGATCA 1 cut(s) 730
Fnu4HI GCNGC 9 cut(s) 140, 143, 146, 149, 173, 704, 753, 801, 904
FokI GGATG 2 cut(s) 50, 566
FriOI GRGCYC 2 cut(s) 285, 314
Fsp4HI GCNGC 9 cut(s) 140, 143, 146, 149, 173, 704, 753, 801, 904
FspBI CTAG 5 cut(s) 113, 339, 387, 686, 744
GluI GCNGC 9 cut(s) 140, 143, 146, 149, 173, 704, 753, 801, 904
GsuI CTGGAG 2 cut(s) 263, 786
HaeIII GGCC 3 cut(s) 312, 351, 619
HapII CCGG 1 cut(s) 204
Hin1II CATG 2 cut(s) 536, 640
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 3 cut(s) 25, 116, 919
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 4 cut(s) 179, 628, 738, 1006
Hpy188III TCNNGA 5 cut(s) 113, 242, 305, 541, 792
HpyAV CCTTC 2 cut(s) 162, 893
HpyCH4III ACNGT 3 cut(s) 486, 865, 943
HpyCH4IV ACGT 1 cut(s) 953
HpyCH4V TGCA 1 cut(s) 755
HpyF10VI GCNNNNNNNGC 8 cut(s) 145, 148, 204, 650, 686, 761, 770, 944
HpyF3I CTNAG 5 cut(s) 176, 363, 627, 828, 973
HpySE526I ACGT 1 cut(s) 953
Hsp92II CATG 2 cut(s) 536, 640
KpnI GGTACC 1 cut(s) 462
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 4 cut(s) 324, 603, 730, 788
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 427
Lsp1109I GCAGC 9 cut(s) 151, 154, 157, 160, 184, 715, 739, 787, 890
LweI GCATC 2 cut(s) 739, 760
MaeI CTAG 5 cut(s) 113, 339, 387, 686, 744
MaeII ACGT 1 cut(s) 953
MaeIII GTNAC 1 cut(s) 541
MalI GATC 4 cut(s) 326, 605, 732, 790
MboI GATC 4 cut(s) 324, 603, 730, 788
MboII GAAGA 6 cut(s) 290, 463, 607, 640, 677, 922
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 2 cut(s) 285, 314
MluCI AATT 4 cut(s) 260, 446, 739, 996
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 5 cut(s) 449, 498, 531, 541, 565
MroNI GCCGGC 1 cut(s) 203
MseI TTAA 2 cut(s) 368, 471
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 3 cut(s) 353, 492, 616
MvaI CCWGG 3 cut(s) 353, 492, 616
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 8 cut(s) 145, 148, 204, 650, 686, 761, 770, 944
NaeI GCCGGC 1 cut(s) 205
NdeI CATATG 1 cut(s) 402
NdeII GATC 4 cut(s) 324, 603, 730, 788
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 2 cut(s) 536, 640
NlaIV GGNNCC 4 cut(s) 208, 312, 460, 577
NmuCI GTSAC 1 cut(s) 541
NruI TCGCGA 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 205
PfeI GAWTC 2 cut(s) 116, 919
PkrI GCNGC 9 cut(s) 141, 144, 147, 150, 174, 705, 754, 802, 905
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PsiI TTATAA 1 cut(s) 1015
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 3 cut(s) 351, 490, 614
PspGI CCWGG 3 cut(s) 351, 490, 614
PspN4I GGNNCC 4 cut(s) 208, 312, 460, 577
PspOMI GGGCCC 1 cut(s) 310
PspPI GGNCC 3 cut(s) 310, 311, 349
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 1 cut(s) 788
RruI TCGCGA 1 cut(s) 306
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SacI GAGCTC 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 2 cut(s) 368, 471
SatI GCNGC 9 cut(s) 140, 143, 146, 149, 173, 704, 753, 801, 904
Sau3AI GATC 4 cut(s) 324, 603, 730, 788
Sau96I GGNCC 3 cut(s) 310, 311, 349
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 3 cut(s) 353, 492, 616
SduI GDGCHC 2 cut(s) 285, 314
SfaNI GCATC 2 cut(s) 739, 760
Sse9I AATT 4 cut(s) 260, 446, 739, 996
SsiI CCGC 3 cut(s) 105, 156, 314
SspI AATATT 1 cut(s) 727
SspMI CTAG 5 cut(s) 113, 339, 387, 686, 744
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 351, 490, 614
StyI CCWWGG 2 cut(s) 7, 579
TaaI ACNGT 3 cut(s) 486, 865, 943
TaiI ACGT 1 cut(s) 956
TaqI TCGA 3 cut(s) 228, 331, 868
TasI AATT 4 cut(s) 260, 446, 739, 996
TfiI GAWTC 2 cut(s) 116, 919
Tru1I TTAA 2 cut(s) 368, 471
Tru9I TTAA 2 cut(s) 368, 471
TscAI CASTG 4 cut(s) 275, 323, 489, 997
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 9 cut(s) 139, 142, 145, 148, 172, 703, 752, 800, 903
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 813, 912
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 4 cut(s) 275, 323, 489, 997
XapI RAATTY 2 cut(s) 260, 739
XbaI TCTAGA 1 cut(s) 112
XspI CTAG 5 cut(s) 113, 339, 387, 686, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.