Rmu_sc0010401.1_g000005

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010401.1
OG0011163
Physical Location & Seq
Reverse (-)
27349 .. 30043
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010401.1_g000005.1.cds

Sequence Viewer

Length: 1437 bp
atgacccaaggacttctccaatggagtccttgccaccactctctctccctcctccccatctccatccccacatccaaacacacccacctccctctctccatctccgccgctctagaatcctccacccaacaacagcagcagcagctctccgccagagagagaaggcagctcagaaacgagaggagagagagcaaagccggcaccaactggaaagaaaaggtcgaggagaagcttctggagaagcccacccagaaattcgctcactggaaagaagagctcaacatcaacaacctcgctcgcgagggcccacagtggtggatcgttcgagtttctagagtcaagggccaggagaccgctcagttaatcgctcggttgctcgccaggaactacccacatatggatttcaaggtttatgctccaacgataccggagaggaggaaactgaagaatggtacctactcggttaaagcaaagccactgtttccggggtgtgcttttttgaggtgtgtgttggacaaggagatacatgatttcgtgaccgagttggatggagttggaggctttattggtgccaaggttggaaatacaaggcagctcagaaacgagaggagagagagcaaagccggcaccaactggaaagaaaaggtcgaggagaagcttctggagaagcccacccagaaattcgctcactggaaagaagagctcaacatcaacaacctcgctcgcgagggcccacagtggtggatcgttcgagtttctagagtcaagggccaggagaccgctcagttaatcgctcggttgctcgccaggaactacccacatatggatttcaaggtttatgctccaacgataccggagaggaggaaactgaagaatggtacctactcggttaaagcaaagccactgtttccggggtgtgcttttttgaggtgtgtgttggacaaggagatacatgatttcgtgaccgagttggatggagttggaggctttattggtgccaaggttggaaatacgaagaggcagatcaacagacccaggccagtatctgagtttgacatggaagctatcttcacaaaggcaaaggaagaacaagaaaaggctgaattagcttttcaacaagagcagcaactcaactctgggctgaatattgatggtctgagttctagtgatgctgcaaaatctgctggagatgccaaatccaaggggagatccagaaaagctgctgatgctctgataaatggttcatctaaggcaaaggataagaaacttgtcctaggttctactgttcgagttgtatctgggacttttgctgaatatgaaggctgcctgaagaagctgaatcgcagaaccaaaaaggcaaccgtggcatttacgttatttgggaaagaaagcttagtagatttagatgtcagtgaaattgtttcagagaccttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

478

Amino Acids

54.09

Weight (kDa)

9.75

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 452, 878
AccB1I GGYRCC 6 cut(s) 200, 452, 569, 626, 878, 995
AccBSI CCGCTC 3 cut(s) 110, 356, 782
AccII CGCG 2 cut(s) 300, 726
AciI CCGC 5 cut(s) 105, 108, 150, 354, 780
AclWI GGATC 3 cut(s) 326, 752, 1203
AcsI RAATTY 2 cut(s) 254, 680
AcuI CTGAAG 3 cut(s) 464, 890, 1349
AfaI GTAC 2 cut(s) 454, 880
AfiI CCNNNNNNNGG 2 cut(s) 397, 823
AgsI TTSAA 3 cut(s) 406, 832, 1115
AjnI CCWGG 5 cut(s) 345, 380, 771, 806, 1034
AleI CACNNNNGTG 2 cut(s) 313, 739
Alw21I GWGCWC 2 cut(s) 279, 705
Alw26I GTCTC 3 cut(s) 344, 770, 1421
AlwI GGATC 3 cut(s) 326, 752, 1203
AlwNI CAGNNNCTG 1 cut(s) 1046
AoxI GGCC 5 cut(s) 304, 343, 730, 769, 1037
ApaI GGGCCC 2 cut(s) 308, 734
ApeKI GCWGC 9 cut(s) 136, 139, 142, 166, 592, 1123, 1172, 1220, 1323
ApoI RAATTY 2 cut(s) 254, 680
Asp718I GGTACC 2 cut(s) 452, 878
AspA2I CCTAGG 1 cut(s) 1273
AspS9I GGNCC 6 cut(s) 304, 305, 343, 730, 731, 769
AsuC2I CCSGG 2 cut(s) 486, 912
AvrII CCTAGG 1 cut(s) 1273
BaeGI GKGCMC 2 cut(s) 308, 734
BanI GGYRCC 6 cut(s) 200, 452, 569, 626, 878, 995
BanII GRGCYC 4 cut(s) 279, 308, 705, 734
Bbv12I GWGCWC 2 cut(s) 279, 705
BbvI GCAGC 9 cut(s) 148, 151, 154, 178, 604, 1135, 1159, 1207, 1310
BccI CCATC 6 cut(s) 65, 71, 107, 542, 968, 1145
BciT130I CCWGG 5 cut(s) 347, 382, 773, 808, 1036
BcnI CCSGG 2 cut(s) 486, 912
BcoDI GTCTC 3 cut(s) 344, 770, 1421
BfaI CTAG 5 cut(s) 113, 333, 759, 1164, 1274
BlnI CCTAGG 1 cut(s) 1273
Bme1390I CCNGG 7 cut(s) 347, 382, 486, 773, 808, 912, 1036
BmgT120I GGNCC 6 cut(s) 304, 305, 343, 730, 731, 769
BmiI GGNNCC 8 cut(s) 202, 306, 454, 571, 628, 732, 880, 997
BmrFI CCNGG 7 cut(s) 347, 382, 486, 773, 808, 912, 1036
BmsI GCATC 3 cut(s) 1159, 1180, 1216
BpmI CTGGAG 3 cut(s) 257, 683, 1206
BpuMI CCSGG 2 cut(s) 486, 912
BsaI GGTCTC 3 cut(s) 344, 770, 1421
BsaJI CCNNGG 9 cut(s) 7, 485, 573, 911, 999, 1034, 1200, 1273, 1362
BsaWI WCCGGW 2 cut(s) 427, 853
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 823
Bse118I RCCGGY 2 cut(s) 197, 623
Bse1I ACTGG 5 cut(s) 212, 269, 638, 695, 1040
BseBI CCWGG 5 cut(s) 347, 382, 773, 808, 1036
BseDI CCNNGG 9 cut(s) 7, 485, 573, 911, 999, 1034, 1200, 1273, 1362
BseGI GGATG 4 cut(s) 63, 71, 553, 979
BseLI CCNNNNNNNGG 2 cut(s) 397, 823
BseMII CTCAG 6 cut(s) 184, 371, 610, 797, 1038, 1148
BseNI ACTGG 5 cut(s) 212, 269, 638, 695, 1040
BseRI GAGGAG 7 cut(s) 41, 196, 239, 448, 622, 665, 874
BseSI GKGCMC 2 cut(s) 308, 734
BseXI GCAGC 9 cut(s) 148, 151, 154, 178, 604, 1135, 1159, 1207, 1310
Bsh1236I CGCG 2 cut(s) 300, 726
BshFI GGCC 5 cut(s) 306, 345, 732, 771, 1039
BshNI GGYRCC 6 cut(s) 200, 452, 569, 626, 878, 995
BsiHKAI GWGCWC 2 cut(s) 279, 705
BsiSI CCGG 6 cut(s) 198, 428, 485, 624, 854, 911
BslFI GGGAC 1 cut(s) 1315
BslI CCNNNNNNNGG 2 cut(s) 397, 823
BsmAI GTCTC 3 cut(s) 344, 770, 1421
BsmFI GGGAC 1 cut(s) 1315
BsnI GGCC 5 cut(s) 306, 345, 732, 771, 1039
Bso31I GGTCTC 3 cut(s) 344, 770, 1421
Bsp120I GGGCCC 2 cut(s) 304, 730
Bsp1286I GDGCHC 4 cut(s) 279, 308, 705, 734
Bsp143I GATC 4 cut(s) 318, 744, 1023, 1208
Bsp68I TCGCGA 2 cut(s) 300, 726
BspACI CCGC 5 cut(s) 105, 108, 150, 354, 780
BspANI GGCC 5 cut(s) 306, 345, 732, 771, 1039
BspCNI CTCAG 6 cut(s) 183, 370, 609, 796, 1039, 1149
BspFNI CGCG 2 cut(s) 300, 726
BspLI GGNNCC 8 cut(s) 202, 306, 454, 571, 628, 732, 880, 997
BspPI GGATC 3 cut(s) 326, 752, 1203
BspQI GCTCTTC 2 cut(s) 267, 693
BspT107I GGYRCC 6 cut(s) 200, 452, 569, 626, 878, 995
BspTNI GGTCTC 3 cut(s) 344, 770, 1421
BsrBI CCGCTC 3 cut(s) 110, 356, 782
BsrFI RCCGGY 2 cut(s) 197, 623
BsrI ACTGG 5 cut(s) 212, 269, 638, 695, 1040
BssAI RCCGGY 2 cut(s) 197, 623
BssECI CCNNGG 9 cut(s) 7, 485, 573, 911, 999, 1034, 1200, 1273, 1362
BssMI GATC 4 cut(s) 318, 744, 1023, 1208
BssT1I CCWWGG 5 cut(s) 7, 573, 999, 1200, 1273
Bst2UI CCWGG 5 cut(s) 347, 382, 773, 808, 1036
Bst4CI ACNGT 6 cut(s) 312, 480, 738, 906, 1285, 1363
Bst6I CTCTTC 3 cut(s) 267, 693, 1010
BstAPI GCANNNNNTGC 1 cut(s) 1181
BstC8I GCNNGC 6 cut(s) 199, 298, 378, 625, 724, 804
BstDEI CTNAG 8 cut(s) 170, 357, 596, 783, 1047, 1157, 1248, 1393
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 4 cut(s) 63, 71, 553, 979
BstFNI CGCG 2 cut(s) 300, 726
BstKTI GATC 4 cut(s) 321, 747, 1026, 1211
BstMAI GTCTC 3 cut(s) 344, 770, 1421
BstMBI GATC 4 cut(s) 318, 744, 1023, 1208
BstMWI GCNNNNNNNGC 8 cut(s) 142, 198, 624, 1106, 1181, 1190, 1226, 1364
BstNI CCWGG 5 cut(s) 347, 382, 773, 808, 1036
BstSCI CCNGG 7 cut(s) 345, 380, 484, 771, 806, 910, 1034
BstSLI GKGCMC 2 cut(s) 308, 734
BstUI CGCG 2 cut(s) 300, 726
BstV1I GCAGC 9 cut(s) 148, 151, 154, 178, 604, 1135, 1159, 1207, 1310
BstX2I RGATCY 1 cut(s) 1208
BstXI CCANNNNNNTGG 2 cut(s) 315, 741
BstYI RGATCY 1 cut(s) 1208
BsuRI GGCC 5 cut(s) 306, 345, 732, 771, 1039
BtgI CCRYGG 1 cut(s) 1362
BtsCI GGATG 4 cut(s) 63, 71, 553, 979
BtsIMutI CAGTG 7 cut(s) 262, 317, 476, 688, 743, 902, 1417
BtuMI TCGCGA 2 cut(s) 300, 726
Cac8I GCNNGC 6 cut(s) 199, 298, 378, 625, 724, 804
CaiI CAGNNNCTG 1 cut(s) 1046
Cfr10I RCCGGY 2 cut(s) 197, 623
Cfr13I GGNCC 6 cut(s) 304, 305, 343, 730, 731, 769
Csp6I GTAC 2 cut(s) 453, 879
CviAII CATG 3 cut(s) 527, 953, 1057
CviQI GTAC 2 cut(s) 453, 879
DdeI CTNAG 8 cut(s) 170, 357, 596, 783, 1047, 1157, 1248, 1393
DpnI GATC 4 cut(s) 320, 746, 1025, 1210
DpnII GATC 4 cut(s) 318, 744, 1023, 1208
Eam1104I CTCTTC 3 cut(s) 267, 693, 1010
EarI CTCTTC 3 cut(s) 267, 693, 1010
EciI GGCGGA 2 cut(s) 94, 139
Ecl136II GAGCTC 2 cut(s) 277, 703
Eco130I CCWWGG 5 cut(s) 7, 573, 999, 1200, 1273
Eco24I GRGCYC 4 cut(s) 279, 308, 705, 734
Eco31I GGTCTC 3 cut(s) 344, 770, 1421
Eco53kI GAGCTC 2 cut(s) 277, 703
Eco57I CTGAAG 3 cut(s) 464, 890, 1349
EcoICRI GAGCTC 2 cut(s) 277, 703
EcoO109I RGGNCCY 2 cut(s) 304, 730
EcoRII CCWGG 5 cut(s) 345, 380, 771, 806, 1034
EcoT14I CCWWGG 5 cut(s) 7, 573, 999, 1200, 1273
EcoT38I GRGCYC 4 cut(s) 279, 308, 705, 734
ErhI CCWWGG 5 cut(s) 7, 573, 999, 1200, 1273
FaeI CATG 3 cut(s) 530, 956, 1060
FaqI GGGAC 1 cut(s) 1315
FatI CATG 3 cut(s) 526, 952, 1056
FauNDI CATATG 2 cut(s) 396, 822
FokI GGATG 4 cut(s) 50, 58, 560, 986
FriOI GRGCYC 4 cut(s) 279, 308, 705, 734
FspBI CTAG 5 cut(s) 113, 333, 759, 1164, 1274
GsuI CTGGAG 3 cut(s) 257, 683, 1206
HaeIII GGCC 5 cut(s) 306, 345, 732, 771, 1039
HapII CCGG 6 cut(s) 198, 428, 485, 624, 854, 911
Hin1II CATG 3 cut(s) 530, 956, 1060
HindIII AAGCTT 3 cut(s) 230, 656, 1390
HinfI GANTC 5 cut(s) 25, 116, 336, 762, 1339
HpaII CCGG 6 cut(s) 198, 428, 485, 624, 854, 911
Hpy188I TCNGA 6 cut(s) 173, 599, 1048, 1158, 1233, 1426
HpyAV CCTTC 2 cut(s) 156, 1313
HpyCH4III ACNGT 6 cut(s) 312, 480, 738, 906, 1285, 1363
HpyCH4IV ACGT 1 cut(s) 1373
HpyCH4V TGCA 1 cut(s) 1175
HpyF10VI GCNNNNNNNGC 8 cut(s) 142, 198, 624, 1106, 1181, 1190, 1226, 1364
HpyF3I CTNAG 8 cut(s) 170, 357, 596, 783, 1047, 1157, 1248, 1393
HpySE526I ACGT 1 cut(s) 1373
Hsp92II CATG 3 cut(s) 530, 956, 1060
KpnI GGTACC 2 cut(s) 456, 882
KroI GCCGGC 2 cut(s) 197, 623
KroNI GCCGGC 2 cut(s) 199, 625
Kzo9I GATC 4 cut(s) 318, 744, 1023, 1208
LguI GCTCTTC 2 cut(s) 267, 693
LmnI GCTCC 2 cut(s) 421, 847
Lsp1109I GCAGC 9 cut(s) 148, 151, 154, 178, 604, 1135, 1159, 1207, 1310
LweI GCATC 3 cut(s) 1159, 1180, 1216
MaeI CTAG 5 cut(s) 113, 333, 759, 1164, 1274
MaeII ACGT 1 cut(s) 1373
MaeIII GTNAC 2 cut(s) 535, 961
MalI GATC 4 cut(s) 320, 746, 1025, 1210
MbiI CCGCTC 3 cut(s) 110, 356, 782
MboI GATC 4 cut(s) 318, 744, 1023, 1208
MboII GAAGA 8 cut(s) 284, 457, 710, 883, 1027, 1060, 1097, 1342
MflI RGATCY 1 cut(s) 1208
MhlI GDGCHC 4 cut(s) 279, 308, 705, 734
MluCI AATT 4 cut(s) 254, 680, 1103, 1416
MlyI GAGTC 3 cut(s) 34, 345, 771
MroNI GCCGGC 2 cut(s) 197, 623
MseI TTAA 4 cut(s) 362, 465, 788, 891
MslI CAYNNNNRTG 2 cut(s) 313, 739
MspI CCGG 6 cut(s) 198, 428, 485, 624, 854, 911
MspR9I CCNGG 7 cut(s) 347, 382, 486, 773, 808, 912, 1036
MvaI CCWGG 5 cut(s) 347, 382, 773, 808, 1036
MvnI CGCG 2 cut(s) 300, 726
MwoI GCNNNNNNNGC 8 cut(s) 142, 198, 624, 1106, 1181, 1190, 1226, 1364
NaeI GCCGGC 2 cut(s) 199, 625
NciI CCSGG 2 cut(s) 486, 912
NdeI CATATG 2 cut(s) 396, 822
NdeII GATC 4 cut(s) 318, 744, 1023, 1208
NgoMIV GCCGGC 2 cut(s) 197, 623
NlaIII CATG 3 cut(s) 530, 956, 1060
NlaIV GGNNCC 8 cut(s) 202, 306, 454, 571, 628, 732, 880, 997
NmuCI GTSAC 2 cut(s) 535, 961
NruI TCGCGA 2 cut(s) 300, 726
OliI CACNNNNGTG 2 cut(s) 313, 739
PciSI GCTCTTC 2 cut(s) 267, 693
PdiI GCCGGC 2 cut(s) 199, 625
PfeI GAWTC 2 cut(s) 116, 1339
PleI GAGTC 3 cut(s) 33, 344, 770
PpsI GAGTC 3 cut(s) 33, 344, 770
Psp124BI GAGCTC 2 cut(s) 279, 705
Psp6I CCWGG 5 cut(s) 345, 380, 771, 806, 1034
PspGI CCWGG 5 cut(s) 345, 380, 771, 806, 1034
PspN4I GGNNCC 8 cut(s) 202, 306, 454, 571, 628, 732, 880, 997
PspOMI GGGCCC 2 cut(s) 304, 730
PspPI GGNCC 6 cut(s) 304, 305, 343, 730, 731, 769
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 1 cut(s) 1208
RruI TCGCGA 2 cut(s) 300, 726
RsaI GTAC 2 cut(s) 454, 880
RsaNI GTAC 2 cut(s) 453, 879
RseI CAYNNNNRTG 2 cut(s) 313, 739
SacI GAGCTC 2 cut(s) 279, 705
SapI GCTCTTC 2 cut(s) 267, 693
SaqAI TTAA 4 cut(s) 362, 465, 788, 891
Sau3AI GATC 4 cut(s) 318, 744, 1023, 1208
Sau96I GGNCC 6 cut(s) 304, 305, 343, 730, 731, 769
SchI GAGTC 3 cut(s) 34, 345, 771
ScrFI CCNGG 7 cut(s) 347, 382, 486, 773, 808, 912, 1036
SduI GDGCHC 4 cut(s) 279, 308, 705, 734
SfaNI GCATC 3 cut(s) 1159, 1180, 1216
SmiMI CAYNNNNRTG 2 cut(s) 313, 739
Sse9I AATT 4 cut(s) 254, 680, 1103, 1416
SsiI CCGC 5 cut(s) 105, 108, 150, 354, 780
SspI AATATT 1 cut(s) 1147
SspMI CTAG 5 cut(s) 113, 333, 759, 1164, 1274
SstI GAGCTC 2 cut(s) 279, 705
StyD4I CCNGG 7 cut(s) 345, 380, 484, 771, 806, 910, 1034
StyI CCWWGG 5 cut(s) 7, 573, 999, 1200, 1273
TaaI ACNGT 6 cut(s) 312, 480, 738, 906, 1285, 1363
TaiI ACGT 1 cut(s) 1376
TaqI TCGA 5 cut(s) 222, 325, 648, 751, 1288
TaqII GACCGA 2 cut(s) 554, 980
TasI AATT 4 cut(s) 254, 680, 1103, 1416
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 116, 1339
Tru1I TTAA 4 cut(s) 362, 465, 788, 891
Tru9I TTAA 4 cut(s) 362, 465, 788, 891
TscAI CASTG 7 cut(s) 269, 317, 483, 695, 743, 909, 1417
TseFI GTSAC 2 cut(s) 535, 961
TseI GCWGC 9 cut(s) 136, 139, 142, 166, 592, 1123, 1172, 1220, 1323
Tsp45I GTSAC 2 cut(s) 535, 961
TspDTI ATGAA 2 cut(s) 1233, 1332
TspRI CASTG 7 cut(s) 269, 317, 483, 695, 743, 909, 1417
XapI RAATTY 2 cut(s) 254, 680
XbaI TCTAGA 3 cut(s) 112, 332, 758
XmaJI CCTAGG 1 cut(s) 1273
XspI CTAG 5 cut(s) 113, 333, 759, 1164, 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.