pycom12g14990

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011163
Physical Location & Seq
Forward (+)
17429856 .. 17432093
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g14990.3

Sequence Viewer

Length: 1209 bp
ATGGAACAACAGAAACCGCGCGAGTTTTTAAAATTTTGGGGACGAAGTATAAAAAGTAACAAGCTTGGTTGGGCTCATTGGGCCCAACAAATCATTAAACGCCAAAGGGACAGAGAATCCGCAACTCCCTCCTTATCTCCCCTCTCTGCAACGCTCGGAAGCTTGAGGATGACACAAGGACTTCTCCCATGGAGTCCTTGCCACCACTCTCTATCTCCCACCTCCCTCTTCGCACTCCCCCTTGCTTTCCCCACTACCAAAAACACCCAATTCCCAGTTTCCATTTCCGCCACTCTAGAACCAGAACCCGCCACCGCCACCGCCACCGCCACCACCCAACAGCTCACAGCCAGAGAGAGAAGGCAGCTCAGGAACGAGAGGAGAGAGAGCAAAACCGGCACCAGCTGGAAAGAGGAGGTGGAGGAGAAGCTTCTGGAGAAGCCCACGAAGAAGTTCGCCAATTGGAAGGAGGAGCTCAACCTTAATAACCTCGCTCATGAGGGCCCGCAATGGTGGATCATCAAGGTCTCTCGAGTCAAGGGCCAGGAGACCGCCCAGCTCATCGCTCGACAGCTCGCCAGAAACTATCCTCATATCGATTTTAAGATATACGCGCCAGCTATCCAAGACAGGAAGAAATTGAAAAATGGTAACTTGGTTAAACCGAGACCCCTATTTCCGGGGTGTGTGTTTATAAAGTGTGTATTGGACAAGGAGATACATGACTTCATAAGAGAGTGCGATGGAGTTGCAGGTTTTGTCGGTTCCAAGGTCGGAAATACGAAAAGACAGATAACCAGACCCCGTCCAGTGTCTGAGTTTGACATGGAAGCTATCTTCAAACAGGCAAAGGAAGAACAACAGAAAGCTGAACAAGCCTTTGAGCAAGAGCAGCAAGAAGCAACCCTCAATGATGCTACCGAATCTGATGGAGATTCTAAACCAAAAAGGCGACCTAGAAAAACACTTGATCCTCTCATAAATGGTTCGTCTAAGGGAAAGAGTGAGAAGCTCGTCCCAGGTTCTTCTGTACGAGTTTTATCTGGTGCTTTTGCAGAATATGTAGGCAGCCTGAAGAAGCTGAATAAAAGAACAAAAAAGGCGACCGTGGGATTTATGCTATTTGGGAAAGAAAGCTTAGTTGATTTAGATGTCAGTGAAATTGTTTTAGAGACCGTTGGAAAAAAAGTTGTTTCAGAGACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

403

Amino Acids

45.42

Weight (kDa)

9.73

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
Acc36I ACCTGC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 398
AccII CGCG 3 cut(s) 19, 21, 614
AciI CCGC 9 cut(s) 17, 120, 288, 309, 315, 321, 327, 506, 552
AclWI GGATC 2 cut(s) 524, 965
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 1094
AfaI GTAC 1 cut(s) 1032
AfiI CCNNNNNNNGG 1 cut(s) 679
AgsI TTSAA 2 cut(s) 643, 841
AjnI CCWGG 2 cut(s) 543, 1018
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 5 cut(s) 532, 542, 661, 1166, 1193
AlwI GGATC 2 cut(s) 524, 965
AlwNI CAGNNNCTG 1 cut(s) 815
Ama87I CYCGRG 1 cut(s) 531
AoxI GGCC 3 cut(s) 81, 502, 541
ApaI GGGCCC 2 cut(s) 85, 506
ApeKI GCWGC 3 cut(s) 364, 892, 1068
ApoI RAATTY 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 452
AspLEI GCGC 2 cut(s) 21, 616
AspS9I GGNCC 5 cut(s) 81, 82, 502, 503, 541
AsuC2I CCSGG 1 cut(s) 681
AvaI CYCGRG 1 cut(s) 531
BaeGI GKGCMC 2 cut(s) 85, 506
BanI GGYRCC 1 cut(s) 398
BanII GRGCYC 4 cut(s) 76, 85, 477, 506
Bbv12I GWGCWC 1 cut(s) 477
BbvI GCAGC 3 cut(s) 376, 904, 1080
BccI CCATC 2 cut(s) 737, 923
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 2 cut(s) 545, 1020
BcnI CCSGG 1 cut(s) 681
BcoDI GTCTC 5 cut(s) 532, 542, 661, 1166, 1193
BfaI CTAG 2 cut(s) 296, 957
BfuAI ACCTGC 1 cut(s) 743
BisI GCNGC 3 cut(s) 365, 893, 1069
BlsI GCNGC 3 cut(s) 366, 894, 1070
Bme1390I CCNGG 3 cut(s) 545, 681, 1020
BmeT110I CYCGRG 1 cut(s) 531
BmgT120I GGNCC 5 cut(s) 81, 82, 502, 503, 541
BmiI GGNNCC 4 cut(s) 83, 400, 504, 766
BmrFI CCNGG 3 cut(s) 545, 681, 1020
BmrI ACTGGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 455
Bpu10I CCTNAGC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 184
BpuMI CCSGG 1 cut(s) 681
Bsa29I ATCGAT 1 cut(s) 597
BsaI GGTCTC 5 cut(s) 532, 542, 661, 1166, 1193
BsaJI CCNNGG 5 cut(s) 188, 680, 768, 1018, 1107
Bsc4I CCNNNNNNNGG 1 cut(s) 679
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 2 cut(s) 275, 809
Bse3DI GCAATG 1 cut(s) 515
BseBI CCWGG 2 cut(s) 545, 1020
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 5 cut(s) 188, 680, 768, 1018, 1107
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 679
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 2 cut(s) 382, 807
BseNI ACTGG 2 cut(s) 275, 809
BseRI GAGGAG 4 cut(s) 394, 428, 437, 485
BseSI GKGCMC 2 cut(s) 85, 506
BseXI GCAGC 3 cut(s) 376, 904, 1080
BseYI CCCAGC 1 cut(s) 555
Bsh1236I CGCG 3 cut(s) 19, 21, 614
Bsh1285I CGRYCG 1 cut(s) 1107
BshFI GGCC 3 cut(s) 83, 504, 543
BshNI GGYRCC 1 cut(s) 398
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 1107
BsiHKAI GWGCWC 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 2 cut(s) 396, 680
BslFI GGGAC 3 cut(s) 54, 122, 1001
BslI CCNNNNNNNGG 1 cut(s) 679
BsmAI GTCTC 5 cut(s) 532, 542, 661, 1166, 1193
BsmFI GGGAC 3 cut(s) 54, 122, 1001
BsnI GGCC 3 cut(s) 83, 504, 543
Bso31I GGTCTC 5 cut(s) 532, 542, 661, 1166, 1193
BsoBI CYCGRG 1 cut(s) 531
Bsp120I GGGCCC 2 cut(s) 81, 502
Bsp1286I GDGCHC 4 cut(s) 76, 85, 477, 506
Bsp143I GATC 2 cut(s) 516, 970
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 9 cut(s) 17, 120, 288, 309, 315, 321, 327, 506, 552
BspANI GGCC 3 cut(s) 83, 504, 543
BspCNI CTCAG 2 cut(s) 381, 808
BspDI ATCGAT 1 cut(s) 597
BspFNI CGCG 3 cut(s) 19, 21, 614
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 4 cut(s) 83, 400, 504, 766
BspMI ACCTGC 1 cut(s) 743
BspPI GGATC 2 cut(s) 524, 965
BspT107I GGYRCC 1 cut(s) 398
BspTNI GGTCTC 5 cut(s) 532, 542, 661, 1166, 1193
BsrDI GCAATG 1 cut(s) 515
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 2 cut(s) 275, 809
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 5 cut(s) 188, 680, 768, 1018, 1107
BssMI GATC 2 cut(s) 516, 970
BssT1I CCWWGG 2 cut(s) 188, 768
Bst2UI CCWGG 2 cut(s) 545, 1020
Bst4CI ACNGT 2 cut(s) 1108, 1177
Bst6I CTCTTC 1 cut(s) 233
BstC8I GCNNGC 3 cut(s) 506, 576, 618
BstDEI CTNAG 4 cut(s) 368, 816, 993, 1138
BstDSI CCRYGG 2 cut(s) 188, 1107
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 3 cut(s) 19, 21, 614
BstHHI GCGC 2 cut(s) 21, 616
BstKTI GATC 2 cut(s) 519, 973
BstMAI GTCTC 5 cut(s) 532, 542, 661, 1166, 1193
BstMBI GATC 2 cut(s) 516, 970
BstMCI CGRYCG 1 cut(s) 1107
BstMWI GCNNNNNNNGC 4 cut(s) 80, 396, 875, 892
BstNI CCWGG 2 cut(s) 545, 1020
BstSCI CCNGG 3 cut(s) 543, 679, 1018
BstSLI GKGCMC 2 cut(s) 85, 506
BstUI CGCG 3 cut(s) 19, 21, 614
BstV1I GCAGC 3 cut(s) 376, 904, 1080
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 3 cut(s) 83, 504, 543
BsuTUI ATCGAT 1 cut(s) 597
BtgI CCRYGG 2 cut(s) 188, 1107
BtgZI GCGATG 2 cut(s) 547, 756
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 2 cut(s) 816, 1162
BveI ACCTGC 1 cut(s) 743
Cac8I GCNNGC 3 cut(s) 506, 576, 618
CaiI CAGNNNCTG 1 cut(s) 815
CciI TCATGA 1 cut(s) 496
CfoI GCGC 2 cut(s) 21, 616
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 5 cut(s) 81, 82, 502, 503, 541
ClaI ATCGAT 1 cut(s) 597
Csp6I GTAC 1 cut(s) 1031
CviAII CATG 5 cut(s) 189, 497, 722, 826, 1204
CviQI GTAC 1 cut(s) 1031
DdeI CTNAG 4 cut(s) 368, 816, 993, 1138
DpnI GATC 2 cut(s) 518, 972
DpnII GATC 2 cut(s) 516, 970
DraI TTTAAA 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
EciI GGCGGA 1 cut(s) 277
Ecl136II GAGCTC 1 cut(s) 475
Eco130I CCWWGG 2 cut(s) 188, 768
Eco24I GRGCYC 4 cut(s) 76, 85, 477, 506
Eco31I GGTCTC 5 cut(s) 532, 542, 661, 1166, 1193
Eco53kI GAGCTC 1 cut(s) 475
Eco57I CTGAAG 1 cut(s) 1094
Eco88I CYCGRG 1 cut(s) 531
EcoICRI GAGCTC 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 543, 1018
EcoT14I CCWWGG 2 cut(s) 188, 768
EcoT38I GRGCYC 4 cut(s) 76, 85, 477, 506
ErhI CCWWGG 2 cut(s) 188, 768
FaeI CATG 5 cut(s) 192, 500, 725, 829, 1207
FaqI GGGAC 3 cut(s) 54, 122, 1001
FatI CATG 5 cut(s) 188, 496, 721, 825, 1203
FauI CCCGC 2 cut(s) 316, 513
Fnu4HI GCNGC 3 cut(s) 365, 893, 1069
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 4 cut(s) 76, 85, 477, 506
Fsp4HI GCNGC 3 cut(s) 365, 893, 1069
FspBI CTAG 2 cut(s) 296, 957
GlaI GCGC 2 cut(s) 20, 615
GluI GCNGC 3 cut(s) 365, 893, 1069
GsaI CCCAGC 1 cut(s) 559
GsuI CTGGAG 1 cut(s) 455
HaeIII GGCC 3 cut(s) 83, 504, 543
HapII CCGG 2 cut(s) 396, 680
HhaI GCGC 2 cut(s) 21, 616
Hin1II CATG 5 cut(s) 192, 500, 725, 829, 1207
Hin6I GCGC 2 cut(s) 19, 614
HinP1I GCGC 2 cut(s) 19, 614
HindIII AAGCTT 4 cut(s) 62, 160, 428, 1135
HinfI GANTC 5 cut(s) 116, 193, 534, 923, 935
HpaII CCGG 2 cut(s) 396, 680
Hpy188I TCNGA 5 cut(s) 158, 776, 817, 928, 1198
Hpy188III TCNNGA 5 cut(s) 296, 370, 434, 497, 531
HpyAV CCTTC 2 cut(s) 354, 460
HpyCH4III ACNGT 2 cut(s) 1108, 1177
HpyCH4V TGCA 3 cut(s) 149, 752, 1055
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 396, 875, 892
HpyF3I CTNAG 4 cut(s) 368, 816, 993, 1138
Hsp92II CATG 5 cut(s) 192, 500, 725, 829, 1207
HspAI GCGC 2 cut(s) 19, 614
Kzo9I GATC 2 cut(s) 516, 970
LmnI GCTCC 1 cut(s) 472
Lsp1109I GCAGC 3 cut(s) 376, 904, 1080
LweI GCATC 1 cut(s) 904
MaeI CTAG 2 cut(s) 296, 957
MaeIII GTNAC 2 cut(s) 56, 650
MalI GATC 2 cut(s) 518, 972
MboI GATC 2 cut(s) 516, 970
MboII GAAGA 7 cut(s) 220, 460, 646, 829, 866, 1017, 1087
MfeI CAATTG 1 cut(s) 460
MhlI GDGCHC 4 cut(s) 76, 85, 477, 506
MluCI AATT 5 cut(s) 32, 269, 460, 638, 1161
MlyI GAGTC 2 cut(s) 202, 543
MmeI TCCRAC 2 cut(s) 754, 1159
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 5 cut(s) 29, 96, 483, 603, 660
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 2 cut(s) 396, 680
MspR9I CCNGG 3 cut(s) 545, 681, 1020
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 2 cut(s) 545, 1020
MvnI CGCG 3 cut(s) 19, 21, 614
MwoI GCNNNNNNNGC 4 cut(s) 80, 396, 875, 892
NciI CCSGG 1 cut(s) 681
NcoI CCATGG 1 cut(s) 188
NdeII GATC 2 cut(s) 516, 970
NlaIII CATG 5 cut(s) 192, 500, 725, 829, 1207
NlaIV GGNNCC 4 cut(s) 83, 400, 504, 766
PaeR7I CTCGAG 1 cut(s) 531
PagI TCATGA 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 3 cut(s) 116, 923, 935
PflFI GACNNNGTC 1 cut(s) 804
PkrI GCNGC 3 cut(s) 366, 894, 1070
PleI GAGTC 2 cut(s) 201, 542
PpsI GAGTC 2 cut(s) 201, 542
PsiI TTATAA 1 cut(s) 695
Psp124BI GAGCTC 1 cut(s) 477
Psp6I CCWGG 2 cut(s) 543, 1018
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 2 cut(s) 543, 1018
PspN4I GGNNCC 4 cut(s) 83, 400, 504, 766
PspOMI GGGCCC 2 cut(s) 81, 502
PspPI GGNCC 5 cut(s) 81, 82, 502, 503, 541
PstNI CAGNNNCTG 1 cut(s) 815
PsyI GACNNNGTC 1 cut(s) 804
PvuII CAGCTG 1 cut(s) 405
RsaI GTAC 1 cut(s) 1032
RsaNI GTAC 1 cut(s) 1031
SacI GAGCTC 1 cut(s) 477
SaqAI TTAA 5 cut(s) 29, 96, 483, 603, 660
SatI GCNGC 3 cut(s) 365, 893, 1069
Sau3AI GATC 2 cut(s) 516, 970
Sau96I GGNCC 5 cut(s) 81, 82, 502, 503, 541
SchI GAGTC 2 cut(s) 202, 543
ScrFI CCNGG 3 cut(s) 545, 681, 1020
SduI GDGCHC 4 cut(s) 76, 85, 477, 506
SfaNI GCATC 1 cut(s) 904
Sfr274I CTCGAG 1 cut(s) 531
SlaI CTCGAG 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 163, 531
SmoI CTYRAG 2 cut(s) 163, 531
Sse9I AATT 5 cut(s) 32, 269, 460, 638, 1161
SsiI CCGC 9 cut(s) 17, 120, 288, 309, 315, 321, 327, 506, 552
SspMI CTAG 2 cut(s) 296, 957
SstI GAGCTC 1 cut(s) 477
StyD4I CCNGG 3 cut(s) 543, 679, 1018
StyI CCWWGG 2 cut(s) 188, 768
TaaI ACNGT 2 cut(s) 1108, 1177
TaqI TCGA 3 cut(s) 532, 568, 597
TasI AATT 5 cut(s) 32, 269, 460, 638, 1161
TfiI GAWTC 3 cut(s) 116, 923, 935
Tru1I TTAA 5 cut(s) 29, 96, 483, 603, 660
Tru9I TTAA 5 cut(s) 29, 96, 483, 603, 660
TscAI CASTG 2 cut(s) 816, 1162
TseI GCWGC 3 cut(s) 364, 892, 1068
TspDTI ATGAA 1 cut(s) 718
TspRI CASTG 2 cut(s) 816, 1162
Tth111I GACNNNGTC 1 cut(s) 804
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 2 cut(s) 296, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.