MD04G1143600.v1.1

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011163
Physical Location & Seq
Forward (+)
23277679 .. 23282201
4523 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1143600.v1.1.491

Sequence Viewer

Length: 963 bp
ATGACACAAGGACTTCTCCCATGGAGTCCTTGCCACCACTCTCTGTCTCCCACCTCCCTCTTCTCACTCCCCCTTGCTTTCCCCATTACCAAACACACCCAATTCCCACATTCCATCTCCGCCACTCTTGAACCCGCCACCGCCGGCACCCAACACCTTACAGCCAGAGAGAGAAGGCAGCTCAGGAACGAGAGGAGAGAGAGCAAAACTGGCACCAGCTGGAAAGAGGAGGTGGAGGAGAAGCTTTTGGAGAAGCCCACGAAGAAGTTCGCCAATTGGAAGGAAGAGCTCAACCTTAATAACCTCGCTCACGAAGGTCCACAATGGTGGATCATCAAGGTCTCTCGAGTCAAGGGCCAGGAGACCGCTCAGCTCATCGCTCGATTGCTCGCCAGGAACTATCCTCAGATTGATTTTAAGATATATGCGCCATCTATCCAAGACAGGAAGAAATTGAAAAATGGTAACTTGGTTAAACCGAGACCTCTATTCCCAGGGTGTGTGTTTATAAGGTGTGTATTGGACAAAGAGTTACATGACTTCATAAGAGAGTGTGATGGAGTTGCGGGCTTTGTCGGTTCCAAGGTCGGAAATACGAAAAGACAAATTACCAGACCCCGGTCAGTTTCTGAGTTTGACATGGAAGCTATCTTCAGACAGGCTAAGGAAGAACAACAGAAAGCTGAACAAGCTTTTGAGCAAGAGCAGCAAGAAGCAGCCCTCAATGATGCTGACGAATCTGCTGGAGATTCTATTTCTAAACCAAAAAGGCGACCTAGAAAAACACTTGATCCTCTCATAAATGGTTCATCTAAAGGAAAGAGTGAGAAGCTCATCCCAGGTTCTTCTGTACGAGTTTTATCTGGGACTTTTGCAGAATATGTAGGCAGCCTGAAGAAGCTGAATAAAAGAACAAAAAAGGTTTGTTTTTTCTGGCATGAAATATTCAGTTTAATTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

321

Amino Acids

36.49

Weight (kDa)

9.7

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusG PF02357 108 - 198 3.8e-09 Transcription termination factor nusG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 509
AccB1I GGYRCC 2 cut(s) 146, 212
AccBSI CCGCTC 1 cut(s) 368
AciI CCGC 5 cut(s) 120, 135, 141, 366, 566
AclWI GGATC 2 cut(s) 338, 785
AcuI CTGAAG 2 cut(s) 637, 914
AfaI GTAC 1 cut(s) 852
AfiI CCNNNNNNNGG 1 cut(s) 618
AgsI TTSAA 2 cut(s) 131, 457
AjnI CCWGG 4 cut(s) 357, 392, 493, 838
AleI CACNNNNGTG 1 cut(s) 325
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 4 cut(s) 51, 346, 356, 475
AlwI GGATC 2 cut(s) 338, 785
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 1 cut(s) 355
ApeKI GCWGC 4 cut(s) 178, 706, 716, 888
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 2 cut(s) 317, 355
AsuC2I CCSGG 1 cut(s) 619
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 1 cut(s) 317
BanI GGYRCC 2 cut(s) 146, 212
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 4 cut(s) 190, 718, 728, 900
BccI CCATC 3 cut(s) 122, 439, 551
BciT130I CCWGG 4 cut(s) 359, 394, 495, 840
BcnI CCSGG 1 cut(s) 619
BcoDI GTCTC 4 cut(s) 51, 346, 356, 475
BfaI CTAG 1 cut(s) 777
BisI GCNGC 4 cut(s) 179, 707, 717, 889
BlpI GCTNAGC 1 cut(s) 369
BlsI GCNGC 4 cut(s) 180, 708, 718, 890
Bme1390I CCNGG 5 cut(s) 359, 394, 495, 619, 840
Bme18I GGWCC 1 cut(s) 317
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 2 cut(s) 317, 355
BmiI GGNNCC 3 cut(s) 148, 214, 580
BmrFI CCNGG 5 cut(s) 359, 394, 495, 619, 840
BmsI GCATC 1 cut(s) 718
BoxI GACNNNNGTC 1 cut(s) 619
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 2 cut(s) 182, 663
Bpu1102I GCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 619
BsaI GGTCTC 3 cut(s) 346, 356, 475
BsaJI CCNNGG 6 cut(s) 20, 493, 494, 582, 617, 838
Bsc4I CCNNNNNNNGG 1 cut(s) 618
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 4 cut(s) 359, 394, 495, 840
BseDI CCNNGG 6 cut(s) 20, 493, 494, 582, 617, 838
BseGI GGATG 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 196, 383, 419, 621
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 3 cut(s) 208, 242, 251
BseXI GCAGC 4 cut(s) 190, 718, 728, 900
BshFI GGCC 1 cut(s) 357
BshNI GGYRCC 2 cut(s) 146, 212
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 2 cut(s) 144, 619
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 1 cut(s) 618
BsmAI GTCTC 4 cut(s) 51, 346, 356, 475
BsmFI GGGAC 1 cut(s) 880
BsnI GGCC 1 cut(s) 357
Bso31I GGTCTC 3 cut(s) 346, 356, 475
BsoBI CYCGRG 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 2 cut(s) 330, 790
Bsp1720I GCTNAGC 1 cut(s) 369
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 5 cut(s) 120, 135, 141, 366, 566
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 4 cut(s) 195, 382, 418, 622
BspLI GGNNCC 3 cut(s) 148, 214, 580
BspPI GGATC 2 cut(s) 338, 785
BspQI GCTCTTC 1 cut(s) 279
BspT107I GGYRCC 2 cut(s) 146, 212
BspTNI GGTCTC 3 cut(s) 346, 356, 475
BsrBI CCGCTC 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 6 cut(s) 20, 493, 494, 582, 617, 838
BssMI GATC 2 cut(s) 330, 790
BssT1I CCWWGG 2 cut(s) 20, 582
Bst2UI CCWGG 4 cut(s) 359, 394, 495, 840
Bst6I CTCTTC 2 cut(s) 65, 279
BstC8I GCNNGC 3 cut(s) 145, 390, 568
BstDEI CTNAG 5 cut(s) 182, 369, 405, 630, 663
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 1 cut(s) 834
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 2 cut(s) 333, 793
BstMAI GTCTC 4 cut(s) 51, 346, 356, 475
BstMBI GATC 2 cut(s) 330, 790
BstMWI GCNNNNNNNGC 3 cut(s) 210, 689, 706
BstNI CCWGG 4 cut(s) 359, 394, 495, 840
BstPAI GACNNNNGTC 1 cut(s) 619
BstSCI CCNGG 5 cut(s) 357, 392, 493, 617, 838
BstV1I GCAGC 4 cut(s) 190, 718, 728, 900
BstXI CCANNNNNNTGG 1 cut(s) 327
BsuRI GGCC 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 20
BtgZI GCGATG 1 cut(s) 361
BtsCI GGATG 1 cut(s) 834
Cac8I GCNNGC 3 cut(s) 145, 390, 568
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 317, 355
Csp6I GTAC 1 cut(s) 851
CviAII CATG 4 cut(s) 21, 536, 640, 938
CviQI GTAC 1 cut(s) 851
DdeI CTNAG 5 cut(s) 182, 369, 405, 630, 663
DpnI GATC 2 cut(s) 332, 792
DpnII GATC 2 cut(s) 330, 790
Eam1104I CTCTTC 2 cut(s) 65, 279
EarI CTCTTC 2 cut(s) 65, 279
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 20, 582
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 3 cut(s) 346, 356, 475
Eco47I GGWCC 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 2 cut(s) 637, 914
Eco88I CYCGRG 1 cut(s) 345
EcoICRI GAGCTC 1 cut(s) 289
EcoRII CCWGG 4 cut(s) 357, 392, 493, 838
EcoT14I CCWWGG 2 cut(s) 20, 582
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 20, 582
FaeI CATG 4 cut(s) 24, 539, 643, 941
FaqI GGGAC 1 cut(s) 880
FatI CATG 4 cut(s) 20, 535, 639, 937
FauI CCCGC 2 cut(s) 142, 559
Fnu4HI GCNGC 4 cut(s) 179, 707, 717, 889
FokI GGATG 1 cut(s) 821
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 4 cut(s) 179, 707, 717, 889
FspBI CTAG 1 cut(s) 777
GlaI GCGC 1 cut(s) 429
GluI GCNGC 4 cut(s) 179, 707, 717, 889
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 1 cut(s) 357
HapII CCGG 2 cut(s) 144, 619
HhaI GCGC 1 cut(s) 430
Hin1II CATG 4 cut(s) 24, 539, 643, 941
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HindIII AAGCTT 2 cut(s) 242, 690
HinfI GANTC 4 cut(s) 25, 348, 737, 749
HpaII CCGG 2 cut(s) 144, 619
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 4 cut(s) 408, 590, 631, 656
Hpy188III TCNNGA 4 cut(s) 128, 184, 311, 345
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 3 cut(s) 168, 274, 308
HpyCH4V TGCA 1 cut(s) 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 689, 706
HpyF3I CTNAG 5 cut(s) 182, 369, 405, 630, 663
Hsp92II CATG 4 cut(s) 24, 539, 643, 941
HspAI GCGC 1 cut(s) 428
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 330, 790
LguI GCTCTTC 1 cut(s) 279
Lsp1109I GCAGC 4 cut(s) 190, 718, 728, 900
LweI GCATC 1 cut(s) 718
MaeI CTAG 1 cut(s) 777
MaeIII GTNAC 2 cut(s) 464, 531
MalI GATC 2 cut(s) 332, 792
MbiI CCGCTC 1 cut(s) 368
MboI GATC 2 cut(s) 330, 790
MboII GAAGA 8 cut(s) 52, 274, 296, 460, 643, 680, 837, 907
MfeI CAATTG 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 5 cut(s) 101, 274, 452, 606, 954
MlyI GAGTC 2 cut(s) 34, 357
MmeI TCCRAC 1 cut(s) 568
MroNI GCCGGC 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 266
MseI TTAA 5 cut(s) 297, 417, 474, 953, 957
MslI CAYNNNNRTG 1 cut(s) 325
MspA1I CMGCKG 1 cut(s) 219
MspI CCGG 2 cut(s) 144, 619
MspR9I CCNGG 5 cut(s) 359, 394, 495, 619, 840
MunI CAATTG 1 cut(s) 274
MvaI CCWGG 4 cut(s) 359, 394, 495, 840
MwoI GCNNNNNNNGC 3 cut(s) 210, 689, 706
NaeI GCCGGC 1 cut(s) 145
NciI CCSGG 1 cut(s) 619
NcoI CCATGG 1 cut(s) 20
NdeII GATC 2 cut(s) 330, 790
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 4 cut(s) 24, 539, 643, 941
NlaIV GGNNCC 3 cut(s) 148, 214, 580
OliI CACNNNNGTG 1 cut(s) 325
PacI TTAATTAA 1 cut(s) 957
PaeR7I CTCGAG 1 cut(s) 345
PasI CCCWGGG 1 cut(s) 494
PciSI GCTCTTC 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 266
PfeI GAWTC 2 cut(s) 737, 749
PkrI GCNGC 4 cut(s) 180, 708, 718, 890
PleI GAGTC 2 cut(s) 33, 356
PpsI GAGTC 2 cut(s) 33, 356
PshAI GACNNNNGTC 1 cut(s) 619
PsiI TTATAA 1 cut(s) 509
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 4 cut(s) 357, 392, 493, 838
PspGI CCWGG 4 cut(s) 357, 392, 493, 838
PspN4I GGNNCC 3 cut(s) 148, 214, 580
PspPI GGNCC 2 cut(s) 317, 355
PstNI CAGNNNCTG 1 cut(s) 629
PvuII CAGCTG 1 cut(s) 219
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
RseI CAYNNNNRTG 1 cut(s) 325
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 279
SaqAI TTAA 5 cut(s) 297, 417, 474, 953, 957
SatI GCNGC 4 cut(s) 179, 707, 717, 889
Sau3AI GATC 2 cut(s) 330, 790
Sau96I GGNCC 2 cut(s) 317, 355
SchI GAGTC 2 cut(s) 34, 357
ScrFI CCNGG 5 cut(s) 359, 394, 495, 619, 840
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 1 cut(s) 718
Sfr274I CTCGAG 1 cut(s) 345
SinI GGWCC 1 cut(s) 317
SlaI CTCGAG 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 325
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 5 cut(s) 101, 274, 452, 606, 954
SsiI CCGC 5 cut(s) 120, 135, 141, 366, 566
SspI AATATT 1 cut(s) 945
SspMI CTAG 1 cut(s) 777
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 5 cut(s) 357, 392, 493, 617, 838
StyI CCWWGG 2 cut(s) 20, 582
TaqI TCGA 2 cut(s) 346, 382
TasI AATT 5 cut(s) 101, 274, 452, 606, 954
TfiI GAWTC 2 cut(s) 737, 749
Tru1I TTAA 5 cut(s) 297, 417, 474, 953, 957
Tru9I TTAA 5 cut(s) 297, 417, 474, 953, 957
TseI GCWGC 4 cut(s) 178, 706, 716, 888
TspDTI ATGAA 3 cut(s) 532, 798, 954
VpaK11BI GGWCC 1 cut(s) 317
XhoI CTCGAG 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 266
XspI CTAG 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.