Rh3BG132200

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011163
Physical Location & Seq
Reverse (-)
11140841 .. 11144175
3335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG132200.1

Sequence Viewer

Length: 1098 bp
ATGACCCAAGGACTTCTCCAATGGAGTCCTTGCCACCGCTCCCTCTCCCTCCTCCCCATCTCCATCCCCACATCCAAACACACCCACCTCCCTCTCTCCATCTCCGCCACTCTAGAATCCTCCACCCAACAACAGCAGCAGCAGCAGCAGCAGCTCTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGAGAGAGAGCAAAGCCGGCACCAACTGGAAAGAAAAGGTCGAGGAGAAGCTTCTGGAGAAGCCCACCCAGAAATTCGCTCACTGGAAGGAAGAGCTCAACATCAACAACCTCGCTCGCGAGGGCCCACAGTGGTGGATCGTTCGAGTTTCTAGAGTCAAGGGCCAGGAGACCGCTCAGTTAATCGCTCGGTTGCTCGCCAGGAACTACCCACATATGGATTTCAAGGTTTATGCTCCAACGATACCGGAGAGGAGGAAACTGAAGAATGGTACCTACTCGGTTAAAGCAAAGCCACTGTTTCCGGGGTGTGCTTTTTTGAGGTGTGTGTTGGACAAGGAGATACATGATTTCGTGACCGAGTTGGATGGAGTTGGAGGCTTTATTGGTGCCAAGGTTGGAAATACGAAGAGGCAGATCAACAGACCCAGGCCAGTATCTGAGTTTGACATGGAAGCTATCTTCGCAAAGGCAAAGGAAGAACAAGAAAAGGCTGAATTAGCTTTTCAACAAGAGCAGCAACTCAACTCTGGACTGAATATTGATGGTCTGAGTTCTAGTGATGCTGCAAAATCTGCTGGAGATGCTAAATCCAAGGGGAGATCCAGAAAAGCTGCTGATGCTCTGATAAGTGGTTCATCTAAGGCAAAGGATAAGAAACTTGTCCTAGGTTCTACTGTTCGAGTTGTATCTGGGACTTTTGCTGAATATGAAGGCTGCCTGAAGAAGCTGAATCGCAGAACCAAAAAGGTATACTGTTTCTGGCATGAATTATTAGACATTAAGTTGATAGCGGAAATATGCATACATGCATTTCTATTTGGGGTGAGCATGGCTGAAATTGTAGTGTCTCGGTATGCATTAATTCACTCAAGGATCTTGACTCAATGCTTGAAATATAAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

365

Amino Acids

41.37

Weight (kDa)

9.74

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusG PF02357 107 - 215 2.6e-06 Transcription termination factor nusG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 461
AccB1I GGYRCC 3 cut(s) 209, 461, 578
AccBSI CCGCTC 2 cut(s) 39, 365
AccI GTMKAC 1 cut(s) 942
AccII CGCG 1 cut(s) 309
AciI CCGC 5 cut(s) 37, 105, 159, 363, 983
AclWI GGATC 3 cut(s) 335, 786, 1073
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 2 cut(s) 473, 932
AfaI GTAC 1 cut(s) 463
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 3 cut(s) 415, 698, 1084
AjnI CCWGG 3 cut(s) 354, 389, 617
AleI CACNNNNGTG 1 cut(s) 322
AluBI AGCT 8 cut(s) 154, 178, 241, 286, 647, 692, 803, 919
AluI AGCT 8 cut(s) 154, 178, 241, 286, 647, 692, 803, 919
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 2 cut(s) 353, 1044
AlwI GGATC 3 cut(s) 335, 786, 1073
AlwNI CAGNNNCTG 1 cut(s) 629
AoxI GGCC 3 cut(s) 313, 352, 620
ApaI GGGCCC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 263
AseI ATTAAT 1 cut(s) 1052
Asp718I GGTACC 1 cut(s) 461
AspA2I CCTAGG 1 cut(s) 856
AspS9I GGNCC 3 cut(s) 313, 314, 352
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 1 cut(s) 1027
AvrII CCTAGG 1 cut(s) 856
BaeGI GKGCMC 1 cut(s) 317
BanI GGYRCC 3 cut(s) 209, 461, 578
BanII GRGCYC 2 cut(s) 288, 317
Bbv12I GWGCWC 1 cut(s) 288
BccI CCATC 5 cut(s) 65, 71, 107, 551, 728
BciT130I CCWGG 3 cut(s) 356, 391, 619
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 2 cut(s) 353, 1044
BfaI CTAG 4 cut(s) 113, 342, 747, 857
BlnI CCTAGG 1 cut(s) 856
Bme1390I CCNGG 4 cut(s) 356, 391, 495, 619
BmgT120I GGNCC 3 cut(s) 313, 314, 352
BmiI GGNNCC 4 cut(s) 211, 315, 463, 580
BmrFI CCNGG 4 cut(s) 356, 391, 495, 619
BmsI GCATC 3 cut(s) 742, 763, 799
BpmI CTGGAG 2 cut(s) 266, 789
BpuEI CTTGAG 1 cut(s) 1045
BpuMI CCSGG 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 6 cut(s) 7, 494, 582, 617, 783, 856
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 221, 278, 623
BseBI CCWGG 3 cut(s) 356, 391, 619
BseDI CCNNGG 6 cut(s) 7, 494, 582, 617, 783, 856
BseGI GGATG 3 cut(s) 63, 71, 562
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 4 cut(s) 193, 380, 621, 731
BseNI ACTGG 3 cut(s) 221, 278, 623
BseRI GAGGAG 4 cut(s) 41, 205, 248, 457
BseSI GKGCMC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 315, 354, 622
BshNI GGYRCC 3 cut(s) 209, 461, 578
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 3 cut(s) 207, 437, 494
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 2 cut(s) 353, 1044
BsmFI GGGAC 1 cut(s) 898
BsnI GGCC 3 cut(s) 315, 354, 622
Bso31I GGTCTC 1 cut(s) 353
Bsp120I GGGCCC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 288, 317
Bsp143I GATC 4 cut(s) 327, 606, 791, 1065
Bsp68I TCGCGA 1 cut(s) 309
BspACI CCGC 5 cut(s) 37, 105, 159, 363, 983
BspANI GGCC 3 cut(s) 315, 354, 622
BspCNI CTCAG 4 cut(s) 192, 379, 622, 732
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 4 cut(s) 211, 315, 463, 580
BspPI GGATC 3 cut(s) 335, 786, 1073
BspQI GCTCTTC 1 cut(s) 276
BspT107I GGYRCC 3 cut(s) 209, 461, 578
BspTNI GGTCTC 1 cut(s) 353
BsrBI CCGCTC 2 cut(s) 39, 365
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 221, 278, 623
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 6 cut(s) 7, 494, 582, 617, 783, 856
BssMI GATC 4 cut(s) 327, 606, 791, 1065
BssNAI GTATAC 1 cut(s) 943
BssT1I CCWWGG 4 cut(s) 7, 582, 783, 856
Bst1107I GTATAC 1 cut(s) 943
Bst2UI CCWGG 3 cut(s) 356, 391, 619
Bst4CI ACNGT 4 cut(s) 321, 489, 868, 947
Bst6I CTCTTC 2 cut(s) 276, 593
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 208, 307, 387
BstDEI CTNAG 5 cut(s) 179, 366, 630, 740, 831
BstF5I GGATG 3 cut(s) 63, 71, 562
BstFNI CGCG 1 cut(s) 309
BstKTI GATC 4 cut(s) 330, 609, 794, 1068
BstMAI GTCTC 2 cut(s) 353, 1044
BstMBI GATC 4 cut(s) 327, 606, 791, 1065
BstNI CCWGG 3 cut(s) 356, 391, 619
BstNSI RCATGY 1 cut(s) 1001
BstSCI CCNGG 4 cut(s) 354, 389, 493, 617
BstSLI GKGCMC 1 cut(s) 317
BstUI CGCG 1 cut(s) 309
BstX2I RGATCY 2 cut(s) 791, 1065
BstXI CCANNNNNNTGG 1 cut(s) 324
BstYI RGATCY 2 cut(s) 791, 1065
BstZ17I GTATAC 1 cut(s) 943
BsuRI GGCC 3 cut(s) 315, 354, 622
BtsCI GGATG 3 cut(s) 63, 71, 562
BtsIMutI CAGTG 3 cut(s) 271, 326, 485
BtuMI TCGCGA 1 cut(s) 309
Cac8I GCNNGC 3 cut(s) 208, 307, 387
CaiI CAGNNNCTG 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 313, 314, 352
Csp6I GTAC 1 cut(s) 462
CviAII CATG 5 cut(s) 536, 640, 956, 998, 1021
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 5 cut(s) 179, 366, 630, 740, 831
DpnI GATC 4 cut(s) 329, 608, 793, 1067
DpnII GATC 4 cut(s) 327, 606, 791, 1065
Eam1104I CTCTTC 2 cut(s) 276, 593
EarI CTCTTC 2 cut(s) 276, 593
EciI GGCGGA 2 cut(s) 94, 148
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 4 cut(s) 7, 582, 783, 856
Eco24I GRGCYC 2 cut(s) 288, 317
Eco31I GGTCTC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 473, 932
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 354, 389, 617
EcoT14I CCWWGG 4 cut(s) 7, 582, 783, 856
EcoT22I ATGCAT 3 cut(s) 995, 1003, 1051
EcoT38I GRGCYC 2 cut(s) 288, 317
ErhI CCWWGG 4 cut(s) 7, 582, 783, 856
FaeI CATG 5 cut(s) 539, 643, 959, 1001, 1024
FaqI GGGAC 1 cut(s) 898
FatI CATG 5 cut(s) 535, 639, 955, 997, 1020
FauNDI CATATG 1 cut(s) 405
FblI GTMKAC 1 cut(s) 942
FokI GGATG 3 cut(s) 50, 58, 569
FriOI GRGCYC 2 cut(s) 288, 317
FspBI CTAG 4 cut(s) 113, 342, 747, 857
GsuI CTGGAG 2 cut(s) 266, 789
HaeIII GGCC 3 cut(s) 315, 354, 622
HapII CCGG 3 cut(s) 207, 437, 494
Hin1II CATG 5 cut(s) 539, 643, 959, 1001, 1024
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 5 cut(s) 25, 116, 345, 922, 1072
HpaII CCGG 3 cut(s) 207, 437, 494
HphI GGTGA 1 cut(s) 1027
Hpy166II GTNNAC 1 cut(s) 943
Hpy188I TCNGA 4 cut(s) 182, 631, 741, 816
Hpy188III TCNNGA 8 cut(s) 113, 245, 308, 342, 544, 720, 795, 1069
Hpy8I GTNNAC 1 cut(s) 943
HpyAV CCTTC 3 cut(s) 165, 271, 896
HpyCH4III ACNGT 4 cut(s) 321, 489, 868, 947
HpyCH4V TGCA 4 cut(s) 758, 993, 1001, 1049
HpyF3I CTNAG 5 cut(s) 179, 366, 630, 740, 831
Hsp92II CATG 5 cut(s) 539, 643, 959, 1001, 1024
KpnI GGTACC 1 cut(s) 465
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 4 cut(s) 327, 606, 791, 1065
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 44, 430
LweI GCATC 3 cut(s) 742, 763, 799
MaeI CTAG 4 cut(s) 113, 342, 747, 857
MaeIII GTNAC 1 cut(s) 544
MalI GATC 4 cut(s) 329, 608, 793, 1067
MbiI CCGCTC 2 cut(s) 39, 365
MboI GATC 4 cut(s) 327, 606, 791, 1065
MboII GAAGA 6 cut(s) 293, 466, 610, 643, 680, 925
MflI RGATCY 2 cut(s) 791, 1065
MhlI GDGCHC 2 cut(s) 288, 317
MluCI AATT 5 cut(s) 263, 686, 959, 1029, 1053
MlyI GAGTC 3 cut(s) 34, 354, 1066
MmeI TCCRAC 5 cut(s) 452, 501, 534, 544, 568
Mph1103I ATGCAT 3 cut(s) 995, 1003, 1051
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 4 cut(s) 371, 474, 972, 1052
MslI CAYNNNNRTG 1 cut(s) 322
MspI CCGG 3 cut(s) 207, 437, 494
MspR9I CCNGG 4 cut(s) 356, 391, 495, 619
MvaI CCWGG 3 cut(s) 356, 391, 619
MvnI CGCG 1 cut(s) 309
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 405
NdeII GATC 4 cut(s) 327, 606, 791, 1065
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 5 cut(s) 539, 643, 959, 1001, 1024
NlaIV GGNNCC 4 cut(s) 211, 315, 463, 580
NmuCI GTSAC 1 cut(s) 544
NruI TCGCGA 1 cut(s) 309
NsiI ATGCAT 3 cut(s) 995, 1003, 1051
NspI RCATGY 1 cut(s) 1001
OliI CACNNNNGTG 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 116, 922
PleI GAGTC 3 cut(s) 33, 353, 1066
PpsI GAGTC 3 cut(s) 33, 353, 1066
PshBI ATTAAT 1 cut(s) 1052
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 354, 389, 617
PspGI CCWGG 3 cut(s) 354, 389, 617
PspN4I GGNNCC 4 cut(s) 211, 315, 463, 580
PspOMI GGGCCC 1 cut(s) 313
PspPI GGNCC 3 cut(s) 313, 314, 352
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 2 cut(s) 791, 1065
RruI TCGCGA 1 cut(s) 309
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
RseI CAYNNNNRTG 1 cut(s) 322
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 4 cut(s) 371, 474, 972, 1052
Sau3AI GATC 4 cut(s) 327, 606, 791, 1065
Sau96I GGNCC 3 cut(s) 313, 314, 352
SchI GAGTC 3 cut(s) 34, 354, 1066
ScrFI CCNGG 4 cut(s) 356, 391, 495, 619
SduI GDGCHC 2 cut(s) 288, 317
SfaNI GCATC 3 cut(s) 742, 763, 799
SmiMI CAYNNNNRTG 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 1060
SmoI CTYRAG 1 cut(s) 1060
Sse9I AATT 5 cut(s) 263, 686, 959, 1029, 1053
SsiI CCGC 5 cut(s) 37, 105, 159, 363, 983
SspI AATATT 1 cut(s) 730
SspMI CTAG 4 cut(s) 113, 342, 747, 857
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 4 cut(s) 354, 389, 493, 617
StyI CCWWGG 4 cut(s) 7, 582, 783, 856
TaaI ACNGT 4 cut(s) 321, 489, 868, 947
TaqI TCGA 3 cut(s) 231, 334, 871
TaqII GACCGA 1 cut(s) 563
TasI AATT 5 cut(s) 263, 686, 959, 1029, 1053
TfiI GAWTC 2 cut(s) 116, 922
Tru1I TTAA 4 cut(s) 371, 474, 972, 1052
Tru9I TTAA 4 cut(s) 371, 474, 972, 1052
TscAI CASTG 3 cut(s) 278, 326, 492
TseFI GTSAC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 3 cut(s) 816, 915, 972
TspRI CASTG 3 cut(s) 278, 326, 492
VspI ATTAAT 1 cut(s) 1052
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 2 cut(s) 112, 341
XceI RCATGY 1 cut(s) 1001
XmaJI CCTAGG 1 cut(s) 856
XmiI GTMKAC 1 cut(s) 942
XspI CTAG 4 cut(s) 113, 342, 747, 857
Zsp2I ATGCAT 3 cut(s) 995, 1003, 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.