Rw3G010820

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011163
Physical Location & Seq
Reverse (-)
10430491 .. 10436052
5562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G010820.1

Sequence Viewer

Length: 834 bp
ATGACCCAAGGGCTTCTCCAATGGGGTCCTTGCCACCGCTCTCTCTCCCTCCTCCCCATCTCCATCCCCACATCCAAACACACCCACCTCCCTCTCTCCATCTCCGCCGCTCTAGAATCCTCCACCCGACAACAGCAGCAGCAGCTCTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGAGAGAGAGCAAAGCCGGCACCAACTGGAAAGAAAAGGTCGAGGAGAAGCTTCTGGAGAAGCCCACCCAGAAATTCGCTCACTGGAAAGAAGAGCTCAACATCAACAACCTCGCTCGCGAGGGCCCACAGTGGTGGATCGTTCGAGTTTCTAGAGTTTATGCTCCAACGATACCGGAGAGGAGGAAATTGAAGAATGGTACCTACTCGGTTAAAGCAAAGCCACTGTTTCCGGGGTGTGCTTTTTTGAGGTGTGTGTTGGACAAGGAGATACATGATTTCGTGACGGAGTTGGATGGAGTTGGAGGCTTTATTGGTGCCAAGGTTGGAAATACGAAGAGGCAGATCAACAGACCCAGGCCAGTATCTGAGTTTGACATGGAAGCTATCTTCGCAAAGGCAAAGGAAGAACAAGAAAAGGCTGAATTAGCTTTTCAACAAGAGCAGCAACTCAACTCTGGGCTGAATATTGATGGTCTGAGTTCTAGGAGATCCAGAAAAGCTGCTGATGCTCTGATAAATGGTTCATCTAAGGCAAAGGATAAGAAACTTGTCCTAGGTTCTACTGTTCGAGTTGTATCTGGGACTTTTACTGAATATGAAGGCTGCCTGAAGAAGCTGAATCGCAGAACCAAAAAGCAAGAAACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

277

Amino Acids

31.49

Weight (kDa)

9.95

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 3 cut(s) 200, 380, 497
AccBSI CCGCTC 2 cut(s) 39, 110
AccII CGCG 1 cut(s) 300
AciI CCGC 4 cut(s) 37, 105, 108, 150
AclWI GGATC 2 cut(s) 326, 666
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 1 cut(s) 382
AgsI TTSAA 2 cut(s) 373, 617
AjnI CCWGG 1 cut(s) 536
AleI CACNNNNGTG 1 cut(s) 313
AluBI AGCT 8 cut(s) 145, 169, 232, 277, 566, 611, 683, 799
AluI AGCT 8 cut(s) 145, 169, 232, 277, 566, 611, 683, 799
Alw21I GWGCWC 1 cut(s) 279
AlwI GGATC 2 cut(s) 326, 666
AlwNI CAGNNNCTG 1 cut(s) 548
AoxI GGCC 2 cut(s) 304, 539
ApaI GGGCCC 1 cut(s) 308
ApeKI GCWGC 7 cut(s) 136, 139, 142, 166, 625, 683, 786
ApoI RAATTY 1 cut(s) 254
Asp718I GGTACC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 736
AspS9I GGNCC 3 cut(s) 26, 304, 305
AsuC2I CCSGG 1 cut(s) 414
AvaII GGWCC 1 cut(s) 26
AvrII CCTAGG 1 cut(s) 736
BaeGI GKGCMC 1 cut(s) 308
BanI GGYRCC 3 cut(s) 200, 380, 497
BanII GRGCYC 2 cut(s) 279, 308
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 7 cut(s) 148, 151, 154, 178, 637, 670, 773
BccI CCATC 5 cut(s) 65, 71, 107, 470, 647
BciT130I CCWGG 1 cut(s) 538
BcnI CCSGG 1 cut(s) 414
BfaI CTAG 4 cut(s) 113, 333, 666, 737
BisI GCNGC 8 cut(s) 108, 137, 140, 143, 167, 626, 684, 787
BlnI CCTAGG 1 cut(s) 736
BlsI GCNGC 8 cut(s) 109, 138, 141, 144, 168, 627, 685, 788
Bme1390I CCNGG 2 cut(s) 414, 538
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 26, 304, 305
BmiI GGNNCC 5 cut(s) 27, 202, 306, 382, 499
BmrFI CCNGG 2 cut(s) 414, 538
BmsI GCATC 1 cut(s) 679
BpmI CTGGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 414
BsaJI CCNNGG 5 cut(s) 7, 413, 501, 536, 736
BsaWI WCCGGW 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 212, 269, 542
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 5 cut(s) 7, 413, 501, 536, 736
BseGI GGATG 3 cut(s) 63, 71, 481
BseMII CTCAG 3 cut(s) 184, 540, 650
BseNI ACTGG 3 cut(s) 212, 269, 542
BseRI GAGGAG 4 cut(s) 41, 196, 239, 376
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 7 cut(s) 148, 151, 154, 178, 637, 670, 773
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 2 cut(s) 306, 541
BshNI GGYRCC 3 cut(s) 200, 380, 497
BsiHKAI GWGCWC 1 cut(s) 279
BsiSI CCGG 3 cut(s) 198, 356, 413
BslFI GGGAC 1 cut(s) 778
BsmFI GGGAC 1 cut(s) 778
BsnI GGCC 2 cut(s) 306, 541
Bsp120I GGGCCC 1 cut(s) 304
Bsp1286I GDGCHC 2 cut(s) 279, 308
Bsp143I GATC 3 cut(s) 318, 525, 671
Bsp68I TCGCGA 1 cut(s) 300
BspACI CCGC 4 cut(s) 37, 105, 108, 150
BspANI GGCC 2 cut(s) 306, 541
BspCNI CTCAG 3 cut(s) 183, 541, 651
BspFNI CGCG 1 cut(s) 300
BspLI GGNNCC 5 cut(s) 27, 202, 306, 382, 499
BspPI GGATC 2 cut(s) 326, 666
BspQI GCTCTTC 1 cut(s) 267
BspT107I GGYRCC 3 cut(s) 200, 380, 497
BsrBI CCGCTC 2 cut(s) 39, 110
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 212, 269, 542
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 5 cut(s) 7, 413, 501, 536, 736
BssMI GATC 3 cut(s) 318, 525, 671
BssT1I CCWWGG 3 cut(s) 7, 501, 736
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 3 cut(s) 312, 408, 748
Bst6I CTCTTC 2 cut(s) 267, 512
BstC8I GCNNGC 2 cut(s) 199, 298
BstDEI CTNAG 4 cut(s) 170, 549, 659, 711
BstF5I GGATG 3 cut(s) 63, 71, 481
BstFNI CGCG 1 cut(s) 300
BstKTI GATC 3 cut(s) 321, 528, 674
BstMBI GATC 3 cut(s) 318, 525, 671
BstMWI GCNNNNNNNGC 5 cut(s) 142, 198, 572, 608, 689
BstNI CCWGG 1 cut(s) 538
BstSCI CCNGG 2 cut(s) 412, 536
BstSLI GKGCMC 1 cut(s) 308
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 7 cut(s) 148, 151, 154, 178, 637, 670, 773
BstX2I RGATCY 1 cut(s) 671
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 1 cut(s) 671
BsuRI GGCC 2 cut(s) 306, 541
BtsCI GGATG 3 cut(s) 63, 71, 481
BtsIMutI CAGTG 3 cut(s) 262, 317, 404
BtuMI TCGCGA 1 cut(s) 300
Cac8I GCNNGC 2 cut(s) 199, 298
CaiI CAGNNNCTG 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 3 cut(s) 26, 304, 305
Csp6I GTAC 1 cut(s) 381
CviAII CATG 2 cut(s) 455, 559
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 4 cut(s) 170, 549, 659, 711
DpnI GATC 3 cut(s) 320, 527, 673
DpnII GATC 3 cut(s) 318, 525, 671
Eam1104I CTCTTC 2 cut(s) 267, 512
EarI CTCTTC 2 cut(s) 267, 512
EciI GGCGGA 2 cut(s) 94, 139
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 3 cut(s) 7, 501, 736
Eco24I GRGCYC 2 cut(s) 279, 308
Eco47I GGWCC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 812
EcoICRI GAGCTC 1 cut(s) 277
EcoO109I RGGNCCY 2 cut(s) 26, 304
EcoRII CCWGG 1 cut(s) 536
EcoT14I CCWWGG 3 cut(s) 7, 501, 736
EcoT38I GRGCYC 2 cut(s) 279, 308
ErhI CCWWGG 3 cut(s) 7, 501, 736
FaeI CATG 2 cut(s) 458, 562
FaiI YATR 4 cut(s) 342, 456, 560, 780
FaqI GGGAC 1 cut(s) 778
FatI CATG 2 cut(s) 454, 558
Fnu4HI GCNGC 8 cut(s) 108, 137, 140, 143, 167, 626, 684, 787
FokI GGATG 3 cut(s) 50, 58, 488
FriOI GRGCYC 2 cut(s) 279, 308
Fsp4HI GCNGC 8 cut(s) 108, 137, 140, 143, 167, 626, 684, 787
FspBI CTAG 4 cut(s) 113, 333, 666, 737
GluI GCNGC 8 cut(s) 108, 137, 140, 143, 167, 626, 684, 787
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 2 cut(s) 306, 541
HapII CCGG 3 cut(s) 198, 356, 413
Hin1II CATG 2 cut(s) 458, 562
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 116, 802
HpaII CCGG 3 cut(s) 198, 356, 413
Hpy188I TCNGA 4 cut(s) 173, 550, 660, 696
Hpy188III TCNNGA 6 cut(s) 113, 236, 299, 333, 463, 675
HpyAV CCTTC 2 cut(s) 156, 776
HpyCH4III ACNGT 3 cut(s) 312, 408, 748
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 198, 572, 608, 689
HpyF3I CTNAG 4 cut(s) 170, 549, 659, 711
Hsp92II CATG 2 cut(s) 458, 562
KpnI GGTACC 1 cut(s) 384
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 318, 525, 671
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 7 cut(s) 148, 151, 154, 178, 637, 670, 773
LweI GCATC 1 cut(s) 679
MaeI CTAG 4 cut(s) 113, 333, 666, 737
MaeIII GTNAC 1 cut(s) 463
MalI GATC 3 cut(s) 320, 527, 673
MbiI CCGCTC 2 cut(s) 39, 110
MboI GATC 3 cut(s) 318, 525, 671
MboII GAAGA 6 cut(s) 284, 385, 529, 562, 599, 805
MfeI CAATTG 1 cut(s) 829
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 2 cut(s) 279, 308
MluCI AATT 4 cut(s) 254, 368, 605, 829
MmeI TCCRAC 5 cut(s) 371, 420, 453, 463, 487
MroNI GCCGGC 1 cut(s) 197
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 313
MspI CCGG 3 cut(s) 198, 356, 413
MspR9I CCNGG 2 cut(s) 414, 538
MunI CAATTG 1 cut(s) 829
MvaI CCWGG 1 cut(s) 538
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 5 cut(s) 142, 198, 572, 608, 689
NaeI GCCGGC 1 cut(s) 199
NciI CCSGG 1 cut(s) 414
NdeII GATC 3 cut(s) 318, 525, 671
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 2 cut(s) 458, 562
NlaIV GGNNCC 5 cut(s) 27, 202, 306, 382, 499
NmuCI GTSAC 1 cut(s) 463
NruI TCGCGA 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 2 cut(s) 116, 802
PkrI GCNGC 8 cut(s) 109, 138, 141, 144, 168, 627, 685, 788
PpuMI RGGWCCY 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 5 cut(s) 27, 202, 306, 382, 499
PspOMI GGGCCC 1 cut(s) 304
PspPI GGNCC 3 cut(s) 26, 304, 305
PspPPI RGGWCCY 1 cut(s) 26
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 1 cut(s) 671
RruI TCGCGA 1 cut(s) 300
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 1 cut(s) 313
SacI GAGCTC 1 cut(s) 279
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 8 cut(s) 108, 137, 140, 143, 167, 626, 684, 787
Sau3AI GATC 3 cut(s) 318, 525, 671
Sau96I GGNCC 3 cut(s) 26, 304, 305
ScrFI CCNGG 2 cut(s) 414, 538
SduI GDGCHC 2 cut(s) 279, 308
SfaNI GCATC 1 cut(s) 679
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 313
Sse9I AATT 4 cut(s) 254, 368, 605, 829
SsiI CCGC 4 cut(s) 37, 105, 108, 150
SspI AATATT 1 cut(s) 649
SspMI CTAG 4 cut(s) 113, 333, 666, 737
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 412, 536
StyI CCWWGG 3 cut(s) 7, 501, 736
TaaI ACNGT 3 cut(s) 312, 408, 748
TaqI TCGA 3 cut(s) 222, 325, 751
TasI AATT 4 cut(s) 254, 368, 605, 829
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 2 cut(s) 116, 802
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 3 cut(s) 269, 317, 411
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 7 cut(s) 136, 139, 142, 166, 625, 683, 786
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 2 cut(s) 696, 795
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 3 cut(s) 269, 317, 411
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 1 cut(s) 254
XbaI TCTAGA 2 cut(s) 112, 332
XmaJI CCTAGG 1 cut(s) 736
XspI CTAG 4 cut(s) 113, 333, 666, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.