RchiOBHm_Chr3g0464151

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011163
Physical Location & Seq
Reverse (-)
11338181 .. 11340697
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43043

Sequence Viewer

Length: 1020 bp
ATGACCCAAGGACTTCTCCAATGGAGTCCTTGCCACCGCTCCCTCTCCCTCCTCCCCATCTCCATCCCCACATCCAAACACACCCACCTCCCTCTCTCCATCTCCGCCACTCTAGAATCCTCCACCCAACAACAGCAGCAGCAGCAGCAGCAGCTCTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGAGAGAGAGCAAAGCCGGCACCAACTGGAAAGAAAAGGTCGAGGAGAAGCTTCTGGAGAAGCCCACCCAGAAATTCGCTCACTGGAAGGAAGAGCTCAACATCAACAACCTCGCTCGCGAGGGCCCACAGTGGTGGATCGTTCGAGTTTCTAGAGTCAAGGGCCAGGAGACCGCTCAGTTAATCGCTCGGTTGCTCGCCAGGAACTACCCACATATGGATTTCAAGGTTTATGCTCCAACGATACCGGAGAGGAGGAAACTGAAGAATGGTACCTACTCGGTTAAAGCAAAGCCACTGTTTCCGGGGTGTGCTTTTTTGAGGTGTGTGTTGGACAAGGAGATACATGATTTCGTGACCGAGTTGGATGGAGTTGGAGGCTTTATTGGTGCCAAGGTTGGAAATACGAAGAGGCAGATCAACAGACCCAGGCCAGTATCTGAGTTTGACATGGAAGCTATCTTCGCAAAGGCAAAGGAAGAACAAGAAAAGGCTGAATTAGCTTTTCAACAAGAGCAGCAACTCAACTCTGGACTGAATATTGATGGTCTGAGTTCTAGTGATGCTGCAAAATCTGCTGGAGATGCTAAATCCAAGGGGAGATCCAGAAAAGCTGCTGATGCTCTGATAAGTGGTTCATCTAAGGCAAAGGATAAGAAACTTGTCCTAGGTTCTACTGTTCGAGTTGTATCTGGGACTTTTGCTGAATATGAAGGCTGCCTGAAGAAGCTGAATCGCAGAACCAAAAAGGCAACCGTGGCATTTACGTTATTTGGGAAAGAAAGCTTAGTAGATTTAGATGTCAGTGAAATTGTTTCAGAGAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

339

Amino Acids

38.03

Weight (kDa)

9.71

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusG PF02357 107 - 215 2.3e-06 Transcription termination factor nusG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 461
AccB1I GGYRCC 3 cut(s) 209, 461, 578
AccBSI CCGCTC 2 cut(s) 39, 365
AccII CGCG 1 cut(s) 309
AciI CCGC 4 cut(s) 37, 105, 159, 363
AclWI GGATC 2 cut(s) 335, 786
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 2 cut(s) 473, 932
AfaI GTAC 1 cut(s) 463
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 2 cut(s) 415, 698
AjnI CCWGG 3 cut(s) 354, 389, 617
AleI CACNNNNGTG 1 cut(s) 322
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 2 cut(s) 335, 786
AlwNI CAGNNNCTG 1 cut(s) 629
AoxI GGCC 3 cut(s) 313, 352, 620
ApaI GGGCCC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 263
Asp718I GGTACC 1 cut(s) 461
AspA2I CCTAGG 1 cut(s) 856
AspS9I GGNCC 3 cut(s) 313, 314, 352
AsuC2I CCSGG 1 cut(s) 495
AvrII CCTAGG 1 cut(s) 856
BaeGI GKGCMC 1 cut(s) 317
BanI GGYRCC 3 cut(s) 209, 461, 578
BanII GRGCYC 2 cut(s) 288, 317
Bbv12I GWGCWC 1 cut(s) 288
BccI CCATC 5 cut(s) 65, 71, 107, 551, 728
BciT130I CCWGG 3 cut(s) 356, 391, 619
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 4 cut(s) 113, 342, 747, 857
BlnI CCTAGG 1 cut(s) 856
Bme1390I CCNGG 4 cut(s) 356, 391, 495, 619
BmgT120I GGNCC 3 cut(s) 313, 314, 352
BmiI GGNNCC 4 cut(s) 211, 315, 463, 580
BmrFI CCNGG 4 cut(s) 356, 391, 495, 619
BmsI GCATC 3 cut(s) 742, 763, 799
BpmI CTGGAG 2 cut(s) 266, 789
BpuMI CCSGG 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 7 cut(s) 7, 494, 582, 617, 783, 856, 945
BsaWI WCCGGW 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 221, 278, 623
BseBI CCWGG 3 cut(s) 356, 391, 619
BseDI CCNNGG 7 cut(s) 7, 494, 582, 617, 783, 856, 945
BseGI GGATG 3 cut(s) 63, 71, 562
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 4 cut(s) 193, 380, 621, 731
BseNI ACTGG 3 cut(s) 221, 278, 623
BseRI GAGGAG 4 cut(s) 41, 205, 248, 457
BseSI GKGCMC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 315, 354, 622
BshNI GGYRCC 3 cut(s) 209, 461, 578
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 3 cut(s) 207, 437, 494
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 898
BsnI GGCC 3 cut(s) 315, 354, 622
Bso31I GGTCTC 1 cut(s) 353
Bsp120I GGGCCC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 288, 317
Bsp143I GATC 3 cut(s) 327, 606, 791
Bsp68I TCGCGA 1 cut(s) 309
BspACI CCGC 4 cut(s) 37, 105, 159, 363
BspANI GGCC 3 cut(s) 315, 354, 622
BspCNI CTCAG 4 cut(s) 192, 379, 622, 732
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 4 cut(s) 211, 315, 463, 580
BspPI GGATC 2 cut(s) 335, 786
BspQI GCTCTTC 1 cut(s) 276
BspT107I GGYRCC 3 cut(s) 209, 461, 578
BspTNI GGTCTC 1 cut(s) 353
BsrBI CCGCTC 2 cut(s) 39, 365
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 221, 278, 623
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 7 cut(s) 7, 494, 582, 617, 783, 856, 945
BssMI GATC 3 cut(s) 327, 606, 791
BssT1I CCWWGG 4 cut(s) 7, 582, 783, 856
Bst2UI CCWGG 3 cut(s) 356, 391, 619
Bst4CI ACNGT 4 cut(s) 321, 489, 868, 946
Bst6I CTCTTC 2 cut(s) 276, 593
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 208, 307, 387
BstDEI CTNAG 6 cut(s) 179, 366, 630, 740, 831, 976
BstDSI CCRYGG 1 cut(s) 945
BstF5I GGATG 3 cut(s) 63, 71, 562
BstFNI CGCG 1 cut(s) 309
BstKTI GATC 3 cut(s) 330, 609, 794
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 3 cut(s) 327, 606, 791
BstNI CCWGG 3 cut(s) 356, 391, 619
BstSCI CCNGG 4 cut(s) 354, 389, 493, 617
BstSLI GKGCMC 1 cut(s) 317
BstUI CGCG 1 cut(s) 309
BstX2I RGATCY 1 cut(s) 791
BstXI CCANNNNNNTGG 1 cut(s) 324
BstYI RGATCY 1 cut(s) 791
BsuRI GGCC 3 cut(s) 315, 354, 622
BtgI CCRYGG 1 cut(s) 945
BtsCI GGATG 3 cut(s) 63, 71, 562
BtsIMutI CAGTG 4 cut(s) 271, 326, 485, 1000
BtuMI TCGCGA 1 cut(s) 309
Cac8I GCNNGC 3 cut(s) 208, 307, 387
CaiI CAGNNNCTG 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 313, 314, 352
Csp6I GTAC 1 cut(s) 462
CviAII CATG 2 cut(s) 536, 640
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 6 cut(s) 179, 366, 630, 740, 831, 976
DpnI GATC 3 cut(s) 329, 608, 793
DpnII GATC 3 cut(s) 327, 606, 791
Eam1104I CTCTTC 2 cut(s) 276, 593
EarI CTCTTC 2 cut(s) 276, 593
EciI GGCGGA 2 cut(s) 94, 148
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 4 cut(s) 7, 582, 783, 856
Eco24I GRGCYC 2 cut(s) 288, 317
Eco31I GGTCTC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 473, 932
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 354, 389, 617
EcoT14I CCWWGG 4 cut(s) 7, 582, 783, 856
EcoT38I GRGCYC 2 cut(s) 288, 317
ErhI CCWWGG 4 cut(s) 7, 582, 783, 856
FaeI CATG 2 cut(s) 539, 643
FaiI YATR 6 cut(s) 405, 407, 423, 537, 641, 900
FaqI GGGAC 1 cut(s) 898
FatI CATG 2 cut(s) 535, 639
FauNDI CATATG 1 cut(s) 405
FokI GGATG 3 cut(s) 50, 58, 569
FriOI GRGCYC 2 cut(s) 288, 317
FspBI CTAG 4 cut(s) 113, 342, 747, 857
GsuI CTGGAG 2 cut(s) 266, 789
HaeIII GGCC 3 cut(s) 315, 354, 622
HapII CCGG 3 cut(s) 207, 437, 494
Hin1II CATG 2 cut(s) 539, 643
HindIII AAGCTT 2 cut(s) 239, 973
HinfI GANTC 4 cut(s) 25, 116, 345, 922
HpaII CCGG 3 cut(s) 207, 437, 494
Hpy188I TCNGA 5 cut(s) 182, 631, 741, 816, 1009
Hpy188III TCNNGA 7 cut(s) 113, 245, 308, 342, 544, 720, 795
HpyAV CCTTC 3 cut(s) 165, 271, 896
HpyCH4III ACNGT 4 cut(s) 321, 489, 868, 946
HpyCH4IV ACGT 1 cut(s) 956
HpyCH4V TGCA 1 cut(s) 758
HpyF3I CTNAG 6 cut(s) 179, 366, 630, 740, 831, 976
HpySE526I ACGT 1 cut(s) 956
Hsp92II CATG 2 cut(s) 539, 643
KpnI GGTACC 1 cut(s) 465
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 3 cut(s) 327, 606, 791
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 2 cut(s) 44, 430
LweI GCATC 3 cut(s) 742, 763, 799
MaeI CTAG 4 cut(s) 113, 342, 747, 857
MaeII ACGT 1 cut(s) 956
MaeIII GTNAC 1 cut(s) 544
MalI GATC 3 cut(s) 329, 608, 793
MbiI CCGCTC 2 cut(s) 39, 365
MboI GATC 3 cut(s) 327, 606, 791
MboII GAAGA 6 cut(s) 293, 466, 610, 643, 680, 925
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 2 cut(s) 288, 317
MluCI AATT 3 cut(s) 263, 686, 999
MlyI GAGTC 2 cut(s) 34, 354
MmeI TCCRAC 5 cut(s) 452, 501, 534, 544, 568
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 2 cut(s) 371, 474
MslI CAYNNNNRTG 1 cut(s) 322
MspI CCGG 3 cut(s) 207, 437, 494
MspR9I CCNGG 4 cut(s) 356, 391, 495, 619
MvaI CCWGG 3 cut(s) 356, 391, 619
MvnI CGCG 1 cut(s) 309
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 405
NdeII GATC 3 cut(s) 327, 606, 791
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 539, 643
NlaIV GGNNCC 4 cut(s) 211, 315, 463, 580
NmuCI GTSAC 1 cut(s) 544
NruI TCGCGA 1 cut(s) 309
OliI CACNNNNGTG 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 116, 922
PleI GAGTC 2 cut(s) 33, 353
PpsI GAGTC 2 cut(s) 33, 353
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 354, 389, 617
PspGI CCWGG 3 cut(s) 354, 389, 617
PspN4I GGNNCC 4 cut(s) 211, 315, 463, 580
PspOMI GGGCCC 1 cut(s) 313
PspPI GGNCC 3 cut(s) 313, 314, 352
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 791
RruI TCGCGA 1 cut(s) 309
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
RseI CAYNNNNRTG 1 cut(s) 322
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 371, 474
Sau3AI GATC 3 cut(s) 327, 606, 791
Sau96I GGNCC 3 cut(s) 313, 314, 352
SchI GAGTC 2 cut(s) 34, 354
ScrFI CCNGG 4 cut(s) 356, 391, 495, 619
SduI GDGCHC 2 cut(s) 288, 317
SfaNI GCATC 3 cut(s) 742, 763, 799
SmiMI CAYNNNNRTG 1 cut(s) 322
Sse9I AATT 3 cut(s) 263, 686, 999
SsiI CCGC 4 cut(s) 37, 105, 159, 363
SspI AATATT 1 cut(s) 730
SspMI CTAG 4 cut(s) 113, 342, 747, 857
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 4 cut(s) 354, 389, 493, 617
StyI CCWWGG 4 cut(s) 7, 582, 783, 856
TaaI ACNGT 4 cut(s) 321, 489, 868, 946
TaiI ACGT 1 cut(s) 959
TaqI TCGA 3 cut(s) 231, 334, 871
TaqII GACCGA 1 cut(s) 563
TasI AATT 3 cut(s) 263, 686, 999
TfiI GAWTC 2 cut(s) 116, 922
Tru1I TTAA 2 cut(s) 371, 474
Tru9I TTAA 2 cut(s) 371, 474
TscAI CASTG 4 cut(s) 278, 326, 492, 1000
TseFI GTSAC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 816, 915
TspRI CASTG 4 cut(s) 278, 326, 492, 1000
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 2 cut(s) 112, 341
XmaJI CCTAGG 1 cut(s) 856
XspI CTAG 4 cut(s) 113, 342, 747, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.