Rroxscaffold_6G00415960

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011163
Physical Location & Seq
Forward (+)
38073144 .. 38076315
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00415960.1

Sequence Viewer

Length: 1020 bp
ATGACCCAAGGACTTCTCCAATGGAGTCCTTGCCACCACTCTCTCTCCCTCCTCCCCATCTCCATCCCCACAACCAAACACACCCACATCCCTCTCTCAATCTCCGCCACTCTAGAATCCTCCACCACCCAACAACAGCAGCAGCAGCAGCAGCTCTCCGCCAGAGAGAGAAGGCAGCTCAGAAACGAGAGGAGAGAGAGCAAAGCCGGCACCAACTGGAAAGAAAAGGTCGAGGAGAAGCTTCTGGAGAAGCCCACCCAGAAATTCGCTCACTGGAAAGAAGAGCTCAACATCAACAACCTCGCTCGCGAGGGCCCACAGTGGTGGATCGTTCGAGTTTCTAGAGTCAAGGGCCAGGAGACGGCTCAGTTAATCGCTCGGTTGCTCGCTAGGAACTACCCACATATGGATTTCAAGGTTTATGCTCCAACGATACCAGAGAGGAGGAAATTGAAGAATGGTACCTACTCGGTGAAAGCAAAGCCACTGTTTCCGGGGTGTGTTTTTTTGAGGTGTGTGTTGGACAAGGAGATACATGATTTCGTGACGGAGTTGGATGGAGTTGGAGGCTTTATTGGTGCCAAGGTTGGAAATACGAAGAGACAGATCAACAGACCCAGGCCAGTATCTGAGTTTGACATGGAAGCTATCTTTGCAAAGGCAAAGGAAGAACAAGAAAAAGCTGAACTAGCTTTTCAAGAAGAGCAGCAACTCAGCTCTGGGCTGAATATTGATGATCTGAATTCTAGTGATGCTGCAAAATCTGCTGGAGATGCTAAATCCAAAGGGAGATCCAGAAAAGCTTCTGATCCTCTGATAAATGGTTCATCTAAGGCAAAGGATAAGAAACTTGTCCTGGGTTCTACTGTTCGAGTTGTATCTGGGACTTTTGCTGAATATGAAGGCTGCCTGAAGAAGCTGAACCGCAGAACCAAAAAGGCAACTGTGGCATTTACGTTATTTGGGAAAGAAAGCTTAGTAGATTTAGATGTCAGTGAAATTGTTTCAGAGACCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

339

Amino Acids

38.17

Weight (kDa)

9.56

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NusG PF02357 107 - 215 8.9e-08 Transcription termination factor nusG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 461
AccB1I GGYRCC 3 cut(s) 209, 461, 578
AccII CGCG 1 cut(s) 309
AciI CCGC 3 cut(s) 105, 159, 925
AclWI GGATC 3 cut(s) 335, 786, 803
AcsI RAATTY 2 cut(s) 263, 742
AcuI CTGAAG 1 cut(s) 932
AfaI GTAC 1 cut(s) 463
AfiI CCNNNNNNNGG 2 cut(s) 361, 406
AgsI TTSAA 3 cut(s) 415, 454, 698
AjnI CCWGG 3 cut(s) 354, 617, 855
AleI CACNNNNGTG 1 cut(s) 322
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 353, 595, 1004
AlwI GGATC 3 cut(s) 335, 786, 803
AlwNI CAGNNNCTG 1 cut(s) 629
AoxI GGCC 3 cut(s) 313, 352, 620
ApaI GGGCCC 1 cut(s) 317
ApeKI GCWGC 9 cut(s) 139, 142, 145, 148, 151, 175, 706, 755, 906
ApoI RAATTY 2 cut(s) 263, 742
Asp700I GAANNNNTTC 1 cut(s) 802
Asp718I GGTACC 1 cut(s) 461
AspS9I GGNCC 3 cut(s) 313, 314, 352
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 317
BanI GGYRCC 3 cut(s) 209, 461, 578
BanII GRGCYC 2 cut(s) 288, 317
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 9 cut(s) 151, 154, 157, 160, 163, 187, 718, 742, 893
BccI CCATC 3 cut(s) 65, 71, 551
BceAI ACGGC 1 cut(s) 378
BciT130I CCWGG 3 cut(s) 356, 619, 857
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 3 cut(s) 353, 595, 1004
BfaI CTAG 5 cut(s) 113, 342, 390, 689, 747
BisI GCNGC 9 cut(s) 140, 143, 146, 149, 152, 176, 707, 756, 907
BlsI GCNGC 9 cut(s) 141, 144, 147, 150, 153, 177, 708, 757, 908
Bme1390I CCNGG 4 cut(s) 356, 495, 619, 857
BmgT120I GGNCC 3 cut(s) 313, 314, 352
BmiI GGNNCC 4 cut(s) 211, 315, 463, 580
BmrFI CCNGG 4 cut(s) 356, 495, 619, 857
BmsI GCATC 2 cut(s) 742, 763
BpmI CTGGAG 2 cut(s) 266, 789
BpuMI CCSGG 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 1004
BsaJI CCNNGG 5 cut(s) 7, 494, 582, 617, 856
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 406
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 221, 278, 623
BseBI CCWGG 3 cut(s) 356, 619, 857
BseDI CCNNGG 5 cut(s) 7, 494, 582, 617, 856
BseGI GGATG 3 cut(s) 63, 87, 562
BseLI CCNNNNNNNGG 2 cut(s) 361, 406
BseMII CTCAG 4 cut(s) 193, 380, 621, 727
BseNI ACTGG 3 cut(s) 221, 278, 623
BseRI GAGGAG 4 cut(s) 41, 205, 248, 457
BseSI GKGCMC 1 cut(s) 317
BseXI GCAGC 9 cut(s) 151, 154, 157, 160, 163, 187, 718, 742, 893
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 315, 354, 622
BshNI GGYRCC 3 cut(s) 209, 461, 578
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 2 cut(s) 207, 494
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 2 cut(s) 361, 406
BsmAI GTCTC 3 cut(s) 353, 595, 1004
BsmBI CGTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 898
BsnI GGCC 3 cut(s) 315, 354, 622
Bso31I GGTCTC 1 cut(s) 1004
Bsp120I GGGCCC 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 288, 317
Bsp143I GATC 5 cut(s) 327, 606, 736, 791, 808
Bsp68I TCGCGA 1 cut(s) 309
BspACI CCGC 3 cut(s) 105, 159, 925
BspANI GGCC 3 cut(s) 315, 354, 622
BspCNI CTCAG 4 cut(s) 192, 379, 622, 726
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 4 cut(s) 211, 315, 463, 580
BspPI GGATC 3 cut(s) 335, 786, 803
BspQI GCTCTTC 2 cut(s) 276, 696
BspT107I GGYRCC 3 cut(s) 209, 461, 578
BspTNI GGTCTC 1 cut(s) 1004
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 221, 278, 623
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 5 cut(s) 7, 494, 582, 617, 856
BssMI GATC 5 cut(s) 327, 606, 736, 791, 808
BssT1I CCWWGG 2 cut(s) 7, 582
Bst2UI CCWGG 3 cut(s) 356, 619, 857
Bst4CI ACNGT 4 cut(s) 321, 489, 868, 946
Bst6I CTCTTC 3 cut(s) 276, 593, 696
BstAPI GCANNNNNTGC 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 208, 307, 387
BstDEI CTNAG 6 cut(s) 179, 366, 630, 713, 831, 976
BstF5I GGATG 3 cut(s) 63, 87, 562
BstFNI CGCG 1 cut(s) 309
BstKTI GATC 5 cut(s) 330, 609, 739, 794, 811
BstMAI GTCTC 3 cut(s) 353, 595, 1004
BstMBI GATC 5 cut(s) 327, 606, 736, 791, 808
BstMWI GCNNNNNNNGC 9 cut(s) 145, 148, 151, 207, 653, 689, 764, 773, 947
BstNI CCWGG 3 cut(s) 356, 619, 857
BstSCI CCNGG 4 cut(s) 354, 493, 617, 855
BstSLI GKGCMC 1 cut(s) 317
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 9 cut(s) 151, 154, 157, 160, 163, 187, 718, 742, 893
BstX2I RGATCY 1 cut(s) 791
BstXI CCANNNNNNTGG 1 cut(s) 324
BstYI RGATCY 1 cut(s) 791
BsuRI GGCC 3 cut(s) 315, 354, 622
BtsCI GGATG 3 cut(s) 63, 87, 562
BtsIMutI CAGTG 4 cut(s) 271, 326, 485, 1000
BtuMI TCGCGA 1 cut(s) 309
Cac8I GCNNGC 3 cut(s) 208, 307, 387
CaiI CAGNNNCTG 1 cut(s) 629
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 313, 314, 352
Csp6I GTAC 1 cut(s) 462
CviAII CATG 2 cut(s) 536, 640
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 6 cut(s) 179, 366, 630, 713, 831, 976
DpnI GATC 5 cut(s) 329, 608, 738, 793, 810
DpnII GATC 5 cut(s) 327, 606, 736, 791, 808
Eam1104I CTCTTC 3 cut(s) 276, 593, 696
EarI CTCTTC 3 cut(s) 276, 593, 696
EciI GGCGGA 2 cut(s) 94, 148
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 7, 582
Eco24I GRGCYC 2 cut(s) 288, 317
Eco31I GGTCTC 1 cut(s) 1004
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 742
EcoRII CCWGG 3 cut(s) 354, 617, 855
EcoT14I CCWWGG 2 cut(s) 7, 582
EcoT38I GRGCYC 2 cut(s) 288, 317
ErhI CCWWGG 2 cut(s) 7, 582
Esp3I CGTCTC 1 cut(s) 353
FaeI CATG 2 cut(s) 539, 643
FaiI YATR 6 cut(s) 405, 407, 423, 537, 641, 900
FaqI GGGAC 1 cut(s) 898
FatI CATG 2 cut(s) 535, 639
FauNDI CATATG 1 cut(s) 405
Fnu4HI GCNGC 9 cut(s) 140, 143, 146, 149, 152, 176, 707, 756, 907
FokI GGATG 3 cut(s) 50, 74, 569
FriOI GRGCYC 2 cut(s) 288, 317
Fsp4HI GCNGC 9 cut(s) 140, 143, 146, 149, 152, 176, 707, 756, 907
FspBI CTAG 5 cut(s) 113, 342, 390, 689, 747
GluI GCNGC 9 cut(s) 140, 143, 146, 149, 152, 176, 707, 756, 907
GsuI CTGGAG 2 cut(s) 266, 789
HaeIII GGCC 3 cut(s) 315, 354, 622
HapII CCGG 2 cut(s) 207, 494
Hin1II CATG 2 cut(s) 539, 643
HindIII AAGCTT 3 cut(s) 239, 801, 973
HinfI GANTC 3 cut(s) 25, 116, 345
HpaII CCGG 2 cut(s) 207, 494
HphI GGTGA 1 cut(s) 484
Hpy188I TCNGA 6 cut(s) 182, 631, 741, 808, 816, 1009
Hpy188III TCNNGA 7 cut(s) 113, 245, 308, 342, 544, 698, 795
HpyAV CCTTC 2 cut(s) 165, 896
HpyCH4III ACNGT 4 cut(s) 321, 489, 868, 946
HpyCH4IV ACGT 1 cut(s) 956
HpyCH4V TGCA 2 cut(s) 656, 758
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 148, 151, 207, 653, 689, 764, 773, 947
HpyF3I CTNAG 6 cut(s) 179, 366, 630, 713, 831, 976
HpySE526I ACGT 1 cut(s) 956
Hsp92II CATG 2 cut(s) 539, 643
KpnI GGTACC 1 cut(s) 465
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 5 cut(s) 327, 606, 736, 791, 808
LguI GCTCTTC 2 cut(s) 276, 696
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 9 cut(s) 151, 154, 157, 160, 163, 187, 718, 742, 893
LweI GCATC 2 cut(s) 742, 763
MaeI CTAG 5 cut(s) 113, 342, 390, 689, 747
MaeII ACGT 1 cut(s) 956
MaeIII GTNAC 1 cut(s) 544
MalI GATC 5 cut(s) 329, 608, 738, 793, 810
MboI GATC 5 cut(s) 327, 606, 736, 791, 808
MboII GAAGA 6 cut(s) 293, 466, 610, 680, 713, 925
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 2 cut(s) 288, 317
MluCI AATT 4 cut(s) 263, 449, 742, 999
MlyI GAGTC 2 cut(s) 34, 354
MmeI TCCRAC 5 cut(s) 452, 501, 534, 544, 568
MroNI GCCGGC 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 802
MseI TTAA 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 322
MspI CCGG 2 cut(s) 207, 494
MspR9I CCNGG 4 cut(s) 356, 495, 619, 857
MvaI CCWGG 3 cut(s) 356, 619, 857
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 9 cut(s) 145, 148, 151, 207, 653, 689, 764, 773, 947
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 405
NdeII GATC 5 cut(s) 327, 606, 736, 791, 808
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 539, 643
NlaIV GGNNCC 4 cut(s) 211, 315, 463, 580
NmuCI GTSAC 1 cut(s) 544
NruI TCGCGA 1 cut(s) 309
OliI CACNNNNGTG 1 cut(s) 322
PciSI GCTCTTC 2 cut(s) 276, 696
PdiI GCCGGC 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 802
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 9 cut(s) 141, 144, 147, 150, 153, 177, 708, 757, 908
PleI GAGTC 2 cut(s) 33, 353
PpsI GAGTC 2 cut(s) 33, 353
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 354, 617, 855
PspGI CCWGG 3 cut(s) 354, 617, 855
PspN4I GGNNCC 4 cut(s) 211, 315, 463, 580
PspOMI GGGCCC 1 cut(s) 313
PspPI GGNCC 3 cut(s) 313, 314, 352
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 1 cut(s) 791
RruI TCGCGA 1 cut(s) 309
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
RseI CAYNNNNRTG 1 cut(s) 322
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 2 cut(s) 276, 696
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 9 cut(s) 140, 143, 146, 149, 152, 176, 707, 756, 907
Sau3AI GATC 5 cut(s) 327, 606, 736, 791, 808
Sau96I GGNCC 3 cut(s) 313, 314, 352
SchI GAGTC 2 cut(s) 34, 354
ScrFI CCNGG 4 cut(s) 356, 495, 619, 857
SduI GDGCHC 2 cut(s) 288, 317
SfaNI GCATC 2 cut(s) 742, 763
SmiMI CAYNNNNRTG 1 cut(s) 322
Sse9I AATT 4 cut(s) 263, 449, 742, 999
SsiI CCGC 3 cut(s) 105, 159, 925
SspI AATATT 1 cut(s) 730
SspMI CTAG 5 cut(s) 113, 342, 390, 689, 747
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 4 cut(s) 354, 493, 617, 855
StyI CCWWGG 2 cut(s) 7, 582
TaaI ACNGT 4 cut(s) 321, 489, 868, 946
TaiI ACGT 1 cut(s) 959
TaqI TCGA 3 cut(s) 231, 334, 871
TasI AATT 4 cut(s) 263, 449, 742, 999
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TscAI CASTG 4 cut(s) 278, 326, 492, 1000
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 9 cut(s) 139, 142, 145, 148, 151, 175, 706, 755, 906
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 816, 915
TspGWI ACGGA 1 cut(s) 563
TspRI CASTG 4 cut(s) 278, 326, 492, 1000
XapI RAATTY 2 cut(s) 263, 742
XbaI TCTAGA 2 cut(s) 112, 341
XmnI GAANNNNTTC 1 cut(s) 802
XspI CTAG 5 cut(s) 113, 342, 390, 689, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.