Rorug03G0070800

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011163
Physical Location & Seq
Forward (+)
5640151 .. 5645777
5627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0070800.1

Sequence Viewer

Length: 1062 bp
ATGCCAAAGATCAAGAGGACTAAGAGAAGCAAGAGGAGAAAGGATGATCATGACGTTGTTGATGTGAGAAGACAAAAATCTCAAAAACTGGATTGCTGCCCCTCAGAAAAATCCCACCTATTCTCGGAATTCTTGGCCGATTCTCGGCGGAGAGTAGTCCTAACCCGGGATCAGTACAAGTATTGCTCCCAGGCTCTCAAATTCTTCCAAGACAAGCTCGACGAGCCTCGGCAGATTATAGAGGAGTTTCGTAAGATAAATAAGGGGAGAGCTGAAACTAGGAAGGAGAAGACTTGCAATGTGGCTCTCAGCAGTGTCAATTGGAGGAAGAACCGATACGATAATATTGTGCCATATGACCAAAACAGGGTTGTTCTGAAGGACTCATCCAAAAGTGGAAGGGACTACATCAATGCCAGCTTCATCACCACAAGTTCGTCTCGGTTTATTGCAACACAAGGTCCACTTCCGAATACGTATGAGGATTTCTGGGAGATGGTGATCCAGTACCGTTGCTCTGTTGTGATTATGCTTACTTGCTTGGACATGGATACATGTGGAGATTATTTTCAGGCAGAAGACGAGAGAGAATTTGGGAATGTGTGTATAGTCACTAAGTGGATGAGAAGTAGTGACACTAATTCTCCATTAGTGTTGCGGCTTTTGGAGGTCAAGCATAAGGAGAACTCTGAGGAGCCACCAGTGTCGGTTTTGCATATTCAGTATCCTGAATGGCCTGATCATGGAGTTCCCACGGACACGGTTGCTGTTCGCGAAATATTGAAAGGATTGATATATCAAGTGGCACCACCGGAGGCTGGCCCGATTGTGGTGCACTGTAGTGCAGGTATTGGGAGAACTGGAACATACTGCACAATTCATGATACGATGAAGAGAATTCTTTCTGGGGACATGTCTGCTTTAGATGTTGCTGATACAGTTGCCACATTCAGGTCTCAGCGAGATGGAATGGTCCAGACGCTGAAGCAATATTGGTTCTGTTATTCTGCGATCATCGATGAATTGGAAGAACTCATCTCGGATCATCAAGATGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

353

Amino Acids

40.89

Weight (kDa)

6.72

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Y_phosphatase PF00102 108 - 339 1.6e-68 Protein-tyrosine phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 836
AccB1I GGYRCC 1 cut(s) 805
AccII CGCG 1 cut(s) 774
AciI CCGC 2 cut(s) 148, 658
AclWI GGATC 3 cut(s) 177, 496, 1050
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 4 cut(s) 128, 200, 590, 897
AcuI CTGAAG 2 cut(s) 398, 1004
AdeI CACNNNGTG 1 cut(s) 618
AfaI GTAC 2 cut(s) 176, 509
AfiI CCNNNNNNNGG 8 cut(s) 124, 144, 166, 367, 743, 818, 829, 951
AflIII ACRYGT 2 cut(s) 554, 912
AgsI TTSAA 1 cut(s) 784
AjnI CCWGG 1 cut(s) 189
AleI CACNNNNGTG 1 cut(s) 840
AluBI AGCT 3 cut(s) 217, 272, 420
AluI AGCT 3 cut(s) 217, 272, 420
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 2 cut(s) 444, 960
Alw44I GTGCAC 1 cut(s) 833
AlwI GGATC 3 cut(s) 177, 496, 1050
AlwNI CAGNNNCTG 1 cut(s) 982
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 3 cut(s) 135, 734, 820
ApaLI GTGCAC 1 cut(s) 833
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 4 cut(s) 128, 200, 590, 897
Asp700I GAANNNNTTC 1 cut(s) 901
AspS9I GGNCC 3 cut(s) 461, 821, 973
AsuC2I CCSGG 2 cut(s) 166, 167
AsuHPI GGTGA 2 cut(s) 418, 511
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 2 cut(s) 461, 973
BaeGI GKGCMC 1 cut(s) 837
BanI GGYRCC 1 cut(s) 805
BarI GAAGNNNNNNTAC 2 cut(s) 320, 352
BbsI GAAGAC 3 cut(s) 76, 296, 585
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 1 cut(s) 83
BccI CCATC 2 cut(s) 490, 959
BcgI CGANNNNNNTGC 2 cut(s) 814, 848
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 2 cut(s) 544, 735
BclI TGATCA 2 cut(s) 46, 739
BcnI CCSGG 2 cut(s) 166, 167
BcoDI GTCTC 2 cut(s) 444, 960
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 838
BfuAI ACCTGC 1 cut(s) 836
BfuI GTATCC 2 cut(s) 544, 735
BisI GCNGC 2 cut(s) 97, 659
BlsI GCNGC 2 cut(s) 98, 660
Bme1390I CCNGG 3 cut(s) 166, 167, 191
Bme18I GGWCC 2 cut(s) 461, 973
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 3 cut(s) 461, 821, 973
BmiI GGNNCC 2 cut(s) 696, 807
BmrFI CCNGG 3 cut(s) 166, 167, 191
BpiI GAAGAC 3 cut(s) 76, 296, 585
BpuMI CCSGG 2 cut(s) 166, 167
Bsa29I ATCGAT 1 cut(s) 1017
BsaAI YACGTR 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 960
BsaJI CCNNGG 4 cut(s) 165, 189, 227, 753
BsaWI WCCGGW 1 cut(s) 811
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 8 cut(s) 124, 144, 166, 367, 743, 818, 829, 951
Bse1I ACTGG 4 cut(s) 93, 505, 701, 865
Bse3DI GCAATG 1 cut(s) 304
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 1 cut(s) 191
BseCI ATCGAT 1 cut(s) 1017
BseDI CCNNGG 4 cut(s) 165, 189, 227, 753
BseGI GGATG 3 cut(s) 49, 386, 627
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 8 cut(s) 124, 144, 166, 367, 743, 818, 829, 951
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 4 cut(s) 117, 322, 681, 971
BseNI ACTGG 4 cut(s) 93, 505, 701, 865
BseRI GAGGAG 3 cut(s) 49, 257, 707
BseSI GKGCMC 1 cut(s) 837
BseXI GCAGC 1 cut(s) 83
BsgI GTGCAG 2 cut(s) 856, 864
Bsh1236I CGCG 1 cut(s) 774
BshFI GGCC 3 cut(s) 137, 736, 822
BshNI GGYRCC 1 cut(s) 805
BshVI ATCGAT 1 cut(s) 1017
BsiHKAI GWGCWC 1 cut(s) 837
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 2 cut(s) 166, 812
BslFI GGGAC 2 cut(s) 416, 923
BslI CCNNNNNNNGG 8 cut(s) 124, 144, 166, 367, 743, 818, 829, 951
BsmAI GTCTC 2 cut(s) 444, 960
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 416, 923
BsnI GGCC 3 cut(s) 137, 736, 822
Bso31I GGTCTC 1 cut(s) 960
BsoBI CYCGRG 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
Bsp68I TCGCGA 1 cut(s) 774
BspACI CCGC 2 cut(s) 148, 658
BspANI GGCC 3 cut(s) 137, 736, 822
BspCNI CTCAG 4 cut(s) 116, 321, 682, 970
BspDI ATCGAT 1 cut(s) 1017
BspFNI CGCG 1 cut(s) 774
BspHI TCATGA 2 cut(s) 49, 880
BspLI GGNNCC 2 cut(s) 696, 807
BspMI ACCTGC 1 cut(s) 836
BspPI GGATC 3 cut(s) 177, 496, 1050
BspT107I GGYRCC 1 cut(s) 805
BspTNI GGTCTC 1 cut(s) 960
BsrDI GCAATG 1 cut(s) 304
BsrI ACTGG 4 cut(s) 93, 505, 701, 865
BssECI CCNNGG 4 cut(s) 165, 189, 227, 753
BssMI GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 4 cut(s) 512, 763, 839, 940
Bst6I CTCTTC 1 cut(s) 887
BstBAI YACGTR 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 418, 820
BstDEI CTNAG 6 cut(s) 21, 103, 308, 615, 690, 957
BstDSI CCRYGG 1 cut(s) 753
BstF5I GGATG 3 cut(s) 49, 386, 627
BstFNI CGCG 1 cut(s) 774
BstKTI GATC 7 cut(s) 12, 49, 172, 504, 742, 1014, 1045
BstMAI GTCTC 2 cut(s) 444, 960
BstMBI GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 2 cut(s) 558, 916
BstSCI CCNGG 3 cut(s) 164, 165, 189
BstSFI CTRYAG 1 cut(s) 838
BstSLI GKGCMC 1 cut(s) 837
BstSNI TACGTA 1 cut(s) 477
BstUI CGCG 1 cut(s) 774
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 3 cut(s) 76, 296, 585
Bsu15I ATCGAT 1 cut(s) 1017
BsuI GTATCC 2 cut(s) 544, 735
BsuRI GGCC 3 cut(s) 137, 736, 822
BsuTUI ATCGAT 1 cut(s) 1017
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 3 cut(s) 49, 386, 627
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 3 cut(s) 319, 708, 835
BtuMI TCGCGA 1 cut(s) 774
BveI ACCTGC 1 cut(s) 836
Cac8I GCNNGC 2 cut(s) 418, 820
CaiI CAGNNNCTG 1 cut(s) 982
CciI TCATGA 2 cut(s) 49, 880
Cfr13I GGNCC 3 cut(s) 461, 821, 973
Cfr9I CCCGGG 1 cut(s) 165
ClaI ATCGAT 1 cut(s) 1017
CseI GACGC 1 cut(s) 988
Csp6I GTAC 2 cut(s) 175, 508
CviAII CATG 6 cut(s) 50, 547, 555, 743, 881, 913
CviQI GTAC 2 cut(s) 175, 508
DdeI CTNAG 6 cut(s) 21, 103, 308, 615, 690, 957
DpnI GATC 7 cut(s) 11, 48, 171, 503, 741, 1013, 1044
DpnII GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
DraIII CACNNNGTG 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
EciI GGCGGA 1 cut(s) 163
Eco105I TACGTA 1 cut(s) 477
Eco31I GGTCTC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 461, 973
Eco57I CTGAAG 2 cut(s) 398, 1004
Eco88I CYCGRG 1 cut(s) 165
EcoRI GAATTC 2 cut(s) 128, 897
EcoRII CCWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 6 cut(s) 53, 550, 558, 746, 884, 916
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FaqI GGGAC 2 cut(s) 416, 923
FatI CATG 6 cut(s) 49, 546, 554, 742, 880, 912
FauNDI CATATG 1 cut(s) 355
FbaI TGATCA 2 cut(s) 46, 739
Fnu4HI GCNGC 2 cut(s) 97, 659
FokI GGATG 3 cut(s) 56, 373, 634
Fsp4HI GCNGC 2 cut(s) 97, 659
FspBI CTAG 1 cut(s) 279
GluI GCNGC 2 cut(s) 97, 659
HaeIII GGCC 3 cut(s) 137, 736, 822
HapII CCGG 2 cut(s) 166, 812
HgaI GACGC 1 cut(s) 988
Hin1II CATG 6 cut(s) 53, 550, 558, 746, 884, 916
HinfI GANTC 2 cut(s) 140, 383
HpaII CCGG 2 cut(s) 166, 812
HphI GGTGA 2 cut(s) 418, 511
Hpy166II GTNNAC 2 cut(s) 464, 835
Hpy188I TCNGA 6 cut(s) 106, 127, 378, 471, 691, 1042
Hpy188III TCNNGA 7 cut(s) 13, 50, 728, 773, 881, 976, 1049
Hpy8I GTNNAC 2 cut(s) 464, 835
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 3 cut(s) 277, 373, 393
HpyCH4III ACNGT 4 cut(s) 512, 763, 839, 940
HpyCH4IV ACGT 2 cut(s) 54, 476
HpyCH4V TGCA 6 cut(s) 297, 452, 715, 835, 845, 873
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 6 cut(s) 21, 103, 308, 615, 690, 957
HpySE526I ACGT 2 cut(s) 54, 476
Hsp92II CATG 6 cut(s) 53, 550, 558, 746, 884, 916
Ksp22I TGATCA 2 cut(s) 46, 739
Kzo9I GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
LmnI GCTCC 2 cut(s) 191, 694
Lsp1109I GCAGC 1 cut(s) 83
MaeI CTAG 1 cut(s) 279
MaeII ACGT 2 cut(s) 54, 476
MaeIII GTNAC 2 cut(s) 610, 632
MalI GATC 7 cut(s) 11, 48, 171, 503, 741, 1013, 1044
MboI GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
MboII GAAGA 7 cut(s) 81, 196, 301, 340, 590, 904, 1040
MfeI CAATTG 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 837
MluCI AATT 8 cut(s) 128, 200, 319, 590, 640, 876, 897, 1022
MlyI GAGTC 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 901
MslI CAYNNNNRTG 2 cut(s) 840, 1050
MspI CCGG 2 cut(s) 166, 812
MspR9I CCNGG 3 cut(s) 166, 167, 191
MunI CAATTG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 774
MwoI GCNNNNNNNGC 1 cut(s) 223
NciI CCSGG 2 cut(s) 166, 167
NdeI CATATG 1 cut(s) 355
NdeII GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
NlaIII CATG 6 cut(s) 53, 550, 558, 746, 884, 916
NlaIV GGNNCC 2 cut(s) 696, 807
NmeAIII GCCGAG 2 cut(s) 124, 208
NmuCI GTSAC 2 cut(s) 610, 632
NruI TCGCGA 1 cut(s) 774
NspI RCATGY 2 cut(s) 558, 916
OliI CACNNNNGTG 1 cut(s) 840
PagI TCATGA 2 cut(s) 49, 880
PciI ACATGT 2 cut(s) 554, 912
PdmI GAANNNNTTC 1 cut(s) 901
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 98, 660
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
Ppu21I YACGTR 1 cut(s) 477
PscI ACATGT 2 cut(s) 554, 912
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 696, 807
PspPI GGNCC 3 cut(s) 461, 821, 973
PstNI CAGNNNCTG 1 cut(s) 982
RruI TCGCGA 1 cut(s) 774
RsaI GTAC 2 cut(s) 176, 509
RsaNI GTAC 2 cut(s) 175, 508
RseI CAYNNNNRTG 2 cut(s) 840, 1050
SatI GCNGC 2 cut(s) 97, 659
Sau3AI GATC 7 cut(s) 9, 46, 169, 501, 739, 1011, 1042
Sau96I GGNCC 3 cut(s) 461, 821, 973
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 3 cut(s) 166, 167, 191
SduI GDGCHC 1 cut(s) 837
SfcI CTRYAG 1 cut(s) 838
SinI GGWCC 2 cut(s) 461, 973
SmaI CCCGGG 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 840, 1050
SnaBI TACGTA 1 cut(s) 477
Sse9I AATT 8 cut(s) 128, 200, 319, 590, 640, 876, 897, 1022
SsiI CCGC 2 cut(s) 148, 658
SspI AATATT 3 cut(s) 346, 780, 992
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 164, 165, 189
TaaI ACNGT 4 cut(s) 512, 763, 839, 940
TaiI ACGT 2 cut(s) 57, 479
TaqI TCGA 2 cut(s) 219, 1017
TasI AATT 8 cut(s) 128, 200, 319, 590, 640, 876, 897, 1022
TatI WGTACW 1 cut(s) 174
TauI GCSGC 1 cut(s) 661
TfiI GAWTC 1 cut(s) 140
TscAI CASTG 3 cut(s) 319, 708, 842
TseFI GTSAC 2 cut(s) 610, 632
TseI GCWGC 1 cut(s) 96
Tsp45I GTSAC 2 cut(s) 610, 632
TspDTI ATGAA 4 cut(s) 412, 869, 905, 1035
TspGWI ACGGA 1 cut(s) 770
TspMI CCCGGG 1 cut(s) 165
TspRI CASTG 3 cut(s) 319, 708, 842
VneI GTGCAC 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 461, 973
XapI RAATTY 4 cut(s) 128, 200, 590, 897
XceI RCATGY 2 cut(s) 558, 916
XmaI CCCGGG 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 901
XspI CTAG 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.