MD12G1157800.v1.1

Transcription termination factor nusG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011163
Physical Location & Seq
Forward (+)
23810430 .. 23820039
9610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1157800.v1.1.491

Sequence Viewer

Length: 636 bp
ATGACACAAGGACTTCTCCCATGGAGTCCTTGCCACCACTCTCTATCTCCCACCTCCATCTTCGCACTCCCCCTTGCTTTCCCCACTACCAAAAACACCCAATTCCCAGTTTCCATTTCCTCCACTCTAGAACCAGAATCAGAACCCGCCACCGCCACCGCCACCACCCAACAACTCACAGCCAGAGAGAGAAGGCAGCTCAGGAACGAGAGGAGAGAGAGCAAAACCGGCACCAGCTGGAAAGAGGAGGTGGAGGAGAAGCTTCTGGAGAAGCCCACGAAGAAGTTCGCCAATTGGAAGGAGGAGCTCAACCTTAATAATCTCGCTCATGAGGGCCCGCAATGGTGGATCATCAAGGTCTCTCGGGTCAAGGGCCAGGAGACCGCTCAGCTCATCGCTCGACAGCTCGCCAGAAACTATCCTCATATCGATTTTAAGGAAAAGACAGATAACCAGACCCCGTCCAGTGCTGAGTTTGACATGGAAGCTGTCTTCAAACAGGAAAAGGAAGAACAACAGAAAGCTGAACAAGCTTTTGAGCAAGAGCAGCAAGAAGCAACCCTCAACGATGCTGCCGAATTTGATGGAGATTCTAAACCAAAAAGCGACCTAGAAAAACACTTGATCCTCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KOW NusG

Protein Analysis

212

Amino Acids

24.18

Weight (kDa)

5.4

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09210
fragaria_vesca FvH4_6g12350 FvH4_6g12350
malus_domestica MD04G1143600.v1.1 MD12G1157800.v1.1
prunus_persica Prupe.6G268100_v2.0.a1 Prupe.6G268100_v2.0.a1
pyrus_communis pycom04g13030 pycom12g14990
rosa_chinensis RchiOBHm_Chr3g0464151
rosa_laevigata RLG00000024718
rosa_multiflora Rmu_sc0010401.1_g000005
rosa_roxburghii Rroxscaffold_6G00415960
rosa_rugosa Rorug03G0070800 Rorug03G0070800
rosa_samantha Rh3BG132200 Rh3CG134400 Rh3DG133400
rosa_wichuraiana Rw3G010820 Rw3G025130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 230
AccBSI CCGCTC 1 cut(s) 386
AciI CCGC 5 cut(s) 147, 153, 159, 338, 384
AclWI GGATC 2 cut(s) 356, 619
AcsI RAATTY 1 cut(s) 578
AgsI TTSAA 1 cut(s) 496
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 9 cut(s) 199, 237, 262, 307, 391, 406, 488, 524, 533
AluI AGCT 9 cut(s) 199, 237, 262, 307, 391, 406, 488, 524, 533
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 364, 374
AlwI GGATC 2 cut(s) 356, 619
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 2 cut(s) 334, 373
ApaI GGGCCC 1 cut(s) 338
ApeKI GCWGC 3 cut(s) 196, 547, 572
ApoI RAATTY 1 cut(s) 578
Asp700I GAANNNNTTC 1 cut(s) 284
AspS9I GGNCC 3 cut(s) 334, 335, 373
AvaI CYCGRG 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 2 cut(s) 309, 338
BbsI GAAGAC 1 cut(s) 484
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 3 cut(s) 208, 559, 559
BccI CCATC 2 cut(s) 65, 578
BciT130I CCWGG 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 364, 374
BfaI CTAG 2 cut(s) 128, 611
BisI GCNGC 3 cut(s) 197, 548, 573
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 3 cut(s) 198, 549, 574
Bme1390I CCNGG 1 cut(s) 377
BmeT110I CYCGRG 1 cut(s) 363
BmgT120I GGNCC 3 cut(s) 334, 335, 373
BmiI GGNNCC 2 cut(s) 232, 336
BmrFI CCNGG 1 cut(s) 377
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 559
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 484
BpmI CTGGAG 1 cut(s) 287
Bpu10I CCTNAGC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 429
BsaI GGTCTC 2 cut(s) 364, 374
BsaJI CCNNGG 1 cut(s) 20
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 2 cut(s) 107, 465
Bse3DI GCAATG 1 cut(s) 347
BseBI CCWGG 1 cut(s) 377
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 347
BseMII CTCAG 3 cut(s) 214, 401, 462
BseNI ACTGG 2 cut(s) 107, 465
BseRI GAGGAG 4 cut(s) 226, 260, 269, 317
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 3 cut(s) 208, 559, 559
BshFI GGCC 2 cut(s) 336, 375
BshNI GGYRCC 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 1 cut(s) 228
BsmAI GTCTC 2 cut(s) 364, 374
BsnI GGCC 2 cut(s) 336, 375
Bso31I GGTCTC 2 cut(s) 364, 374
BsoBI CYCGRG 1 cut(s) 363
Bsp120I GGGCCC 1 cut(s) 334
Bsp1286I GDGCHC 2 cut(s) 309, 338
Bsp143I GATC 2 cut(s) 348, 624
Bsp1720I GCTNAGC 1 cut(s) 387
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 5 cut(s) 147, 153, 159, 338, 384
BspANI GGCC 2 cut(s) 336, 375
BspCNI CTCAG 3 cut(s) 213, 400, 463
BspDI ATCGAT 1 cut(s) 429
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 232, 336
BspPI GGATC 2 cut(s) 356, 619
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 2 cut(s) 364, 374
BsrBI CCGCTC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 2 cut(s) 107, 465
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 20
BssMI GATC 2 cut(s) 348, 624
BssT1I CCWWGG 1 cut(s) 20
Bst2UI CCWGG 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 338, 408
BstDEI CTNAG 3 cut(s) 200, 387, 471
BstDSI CCRYGG 1 cut(s) 20
BstKTI GATC 2 cut(s) 351, 627
BstMAI GTCTC 2 cut(s) 364, 374
BstMBI GATC 2 cut(s) 348, 624
BstMWI GCNNNNNNNGC 3 cut(s) 228, 530, 547
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 375
BstSLI GKGCMC 1 cut(s) 338
BstV1I GCAGC 3 cut(s) 208, 559, 559
BstV2I GAAGAC 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 2 cut(s) 336, 375
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 20
BtgZI GCGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 338, 408
CciI TCATGA 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 334, 335, 373
ClaI ATCGAT 1 cut(s) 429
CviAII CATG 3 cut(s) 21, 329, 481
DdeI CTNAG 3 cut(s) 200, 387, 471
DpnI GATC 2 cut(s) 350, 626
DpnII GATC 2 cut(s) 348, 624
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 20
Eco24I GRGCYC 2 cut(s) 309, 338
Eco31I GGTCTC 2 cut(s) 364, 374
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 20
EcoT38I GRGCYC 2 cut(s) 309, 338
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 3 cut(s) 24, 332, 484
FaiI YATR 5 cut(s) 22, 330, 426, 482, 634
FatI CATG 3 cut(s) 20, 328, 480
FauI CCCGC 2 cut(s) 154, 345
Fnu4HI GCNGC 3 cut(s) 197, 548, 573
FriOI GRGCYC 2 cut(s) 309, 338
Fsp4HI GCNGC 3 cut(s) 197, 548, 573
FspBI CTAG 2 cut(s) 128, 611
GluI GCNGC 3 cut(s) 197, 548, 573
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 336, 375
HapII CCGG 1 cut(s) 228
Hin1II CATG 3 cut(s) 24, 332, 484
HindIII AAGCTT 2 cut(s) 260, 531
HinfI GANTC 3 cut(s) 25, 137, 590
HpaII CCGG 1 cut(s) 228
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 4 cut(s) 128, 202, 266, 329
HpyAV CCTTC 2 cut(s) 186, 292
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 530, 547
HpyF3I CTNAG 3 cut(s) 200, 387, 471
Hsp92II CATG 3 cut(s) 24, 332, 484
Kzo9I GATC 2 cut(s) 348, 624
LmnI GCTCC 1 cut(s) 304
Lsp1109I GCAGC 3 cut(s) 208, 559, 559
LweI GCATC 1 cut(s) 559
MaeI CTAG 2 cut(s) 128, 611
MalI GATC 2 cut(s) 350, 626
MbiI CCGCTC 1 cut(s) 386
MboI GATC 2 cut(s) 348, 624
MboII GAAGA 4 cut(s) 52, 292, 484, 521
MfeI CAATTG 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 309, 338
MluCI AATT 3 cut(s) 101, 292, 578
MlyI GAGTC 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 284
MseI TTAA 2 cut(s) 315, 435
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 377
MunI CAATTG 1 cut(s) 292
MvaI CCWGG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 228, 530, 547
NcoI CCATGG 1 cut(s) 20
NdeII GATC 2 cut(s) 348, 624
NlaIII CATG 3 cut(s) 24, 332, 484
NlaIV GGNNCC 2 cut(s) 232, 336
PagI TCATGA 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 137, 590
PflFI GACNNNGTC 1 cut(s) 460
PkrI GCNGC 3 cut(s) 198, 549, 574
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 2 cut(s) 232, 336
PspOMI GGGCCC 1 cut(s) 334
PspPI GGNCC 3 cut(s) 334, 335, 373
PsyI GACNNNGTC 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 237
SacI GAGCTC 1 cut(s) 309
SaqAI TTAA 2 cut(s) 315, 435
SatI GCNGC 3 cut(s) 197, 548, 573
Sau3AI GATC 2 cut(s) 348, 624
Sau96I GGNCC 3 cut(s) 334, 335, 373
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 2 cut(s) 309, 338
SfaNI GCATC 1 cut(s) 559
Sse9I AATT 3 cut(s) 101, 292, 578
SsiI CCGC 5 cut(s) 147, 153, 159, 338, 384
SspMI CTAG 2 cut(s) 128, 611
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 20
TaqI TCGA 2 cut(s) 400, 429
TasI AATT 3 cut(s) 101, 292, 578
TfiI GAWTC 2 cut(s) 137, 590
Tru1I TTAA 2 cut(s) 315, 435
Tru9I TTAA 2 cut(s) 315, 435
TscAI CASTG 1 cut(s) 472
TseI GCWGC 3 cut(s) 196, 547, 572
TspRI CASTG 1 cut(s) 472
Tth111I GACNNNGTC 1 cut(s) 460
XapI RAATTY 1 cut(s) 578
XbaI TCTAGA 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 2 cut(s) 128, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.