FvH4_6g16040

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002662
Physical Location & Seq
Forward (+)
10058732 .. 10066778
8047 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16040.t6

Sequence Viewer

Length: 4059 bp
ATGGAGTCTGCTAGCTGTATTGCTAACAATAGACCAGGTGCTGTCCATCAGTTGCTTCCTGTTGTTGGACCTATGCTTCTGATTGCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGTTGAAGCAGGTTCCCGATATGGTACTGATCTGGCTGCAGTGATGTTTATATTCAATTTGGCTGCTATTTTGTGTCACTATCTGTCTGCTCGGATTGCTGTAGTCACTGGAAGAGATCTTGCTCAGATTTGTAGTGAAGAGTATGACAAGGCTACATGCATATTCTTAGGCGTACAAACAGAGATGTCGGTGATTTTGTTGGACCTTACCATGATCCTCGGTATCGCACATGGACTTAATCTTCTGTTTGGGTGGGACTTGTTCACTTGTGTGTTTTTGACTGCTGCTAATGCTGTTTTATACCCTCTTTTTTCCACCCTCCTGGACACTTGCAAGGCGAAATTCCTATGTGTGTGCATATACGTTGCAGGATTCATACTGCTTTCCTTTGTTCTTGGAGTATTCATCAGTCAACCGCAAATGCCACTTTCCATGACTGGGATGCTAACAAAATTGAGTGGGGAAAGTGCTTTTTCACTGATGAGTCTTCTTGGAGCTAGTATTATGCCCCACAGTTTTTATCTTCATTCTTCTATTGTGCAGCAGCATCAGCAACAACAAACTGTTTCTAAGGATACTTTGTGTCAGAACCATTTTGTCGCCATCTTCTGCATGTTTAATGGTATTTATTTGGTGAATTATGTTCTGATGACCTTAGCAGCAAATGCATTCTACACTTCACGTGGTCTTCTTACTTTTCAGGATGCAATGTCCCTTATTGAACAGGTCTTTTGGGGTCCAATAGTTCCTGTTGCCTTCTTGCTGGTTCTCTTTTTATCAAATCAAATCACAACATTAAGCTGGAGTCTGGGTGGACAAGTAGTCTTGAATGACTTCTTAAAACTGGACCTTCCTGGTTGGCTTCACTGTGCTACAATCAGAATCATTGCTGTTGTTCCTGCTCTGTATTTTGTTTGGAGCTCAGGAGCTGAGGGGATGTATCAACTGCTTGTATCTACACAGGTGTTGGCAGCCCTGCTACTGCCATCTTCTGTGATCCCTCTTTTTCGTGTTGCTGCTTCAAGACAATTAATGGGAGCCCATAAGATTTCTCAGTTCGTTGAATTTTCAGCCCTCATTACACTTATTGGGATGCTTGGATTAAAAGTTGTCTTTGTAGTGGAGATGATTTTTGGGAATAGTGATTGGGTGGATAATTTGAGATGGGATGCTGGGAGTAGTATGTCTGTACTTCTCATCACTGCTTCTGCATCATTTTGCCTGATGATTTGGTTGGCAGCCACACCGTTAAAATCTGCAAGTGCTCGAATAGAGAACCAGGTGTGGAACTGGGATATGCCTAAGGGTGTCTCGGAGCCATTTAGAAATAAGGAGACTGATATAGCCGAACATAACTATCATAGAGATGCAGATATACAGAAGCATGAACCATCACCATCATCTGGGGACGCATTGGATAGAGAATTAGATACAGCCGTTGCAAATTTTGATTTTGTTCTGCCTGAAACTCTCTTGGAGCCTGATCAGGAGCTTCAACTATCTGGTGTAGAGGAAAATAGTTCTCTTGGTACCTTTCCTCACTCTGCCAAATGCAGCAAGGAGGAACCCACACCTGTAGTAGAATTAACTCGCGTCCCAACTGTGGCTAATGAGGTTTCTGATGTTACAGTACTGGGCACCGATACTGTGAAATTTGAATCAACAGAGCAAGTTGAGAAGACTCTTGCAACTGAAGGAGACTTGCCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAACCAGAAGATTCCTTGAAAGAGGCTTCAGAGAGCACCACGTTAACATCTGAGGGTCCAGGATCATTCAGGAGTCTCAGCGCAAAAGGTGACGAAGGGGGGAGCGGTGCTGGAAGCCTCTCAAGACTAGCAGGGTTGGGCCGTGCTGCTAGACGTCAACTAGCTGCTGCACTCGATGAGTTTTGGGGACAACTGTATGATTTCCATGGGAATGTAATTAAAGAAGCAAGAACAAAGAAACTGGATCTGTTGTTGGGGTCAGATTCAAAGGCTTCTTCTGCTGCTTCCTCTGCTTCTTCCTTGCTGAAAGATGATACCACTGCAAAAGAAGTTTCTGGATGCTTTCCATCTGTAGGAGGCAAAGGATCTGATCCTTTAATCAACTTGAGTTTATATGACTCTGTAAATCAGCAGAGGCTGCAAAACAGTATAGAGTCAGCATATGGCGCTCAAAGGGGATCTTCCTTGTTATGGCCCGGCCACATGCACTTGCTGGATGCGTATGTCCAAAATTCAAGCCGCAGTGTTATTGACTTGGGTGAGAGGCGCTATTCAAGTGTGCACAGTATACCATCTTCTGACTTGGGTGAGAGGCGCTATTCCAGTGTGCGCAGTATACCATCTTCTGACTTGGGTGAGCGGCGCTATTCAAGTGTGCGCAGTATACCAACTTCTGACTTGGGTGAGAGGCGCTATTCAAGTGTGCGGAGCATACCATCTGCTGAGAGCTGGGATTACCAGCCAGCCACAGTACATGGTTATCAGATGCCATCGTATCTTAACCGTAATGACAGAAGTTCCAGTAACTTGAATGGTCAAATAGAGTCACCAGCCCTAAATTCTGCTTCTTCATTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGCTCTGTTCAGGCTTCCAGTTTTCAGAACCTTACAGTATCTCGACAAAGTCCATTGCAATCTGATAGACCATATTATGATGTACCCTCCTCTGGAATTTCGGAGAATGCGGTAAACTCAGCCAATGCAAAGAAATACCACAGTCTACCTGACATTAACCGTGATCTCTACAACTCTAGTAAAAGTGCTCCACGGGATCCTCCTCCTGGGTTTGGGATAATGGGTTATGAATCATCCTTGTATCCAAAATCTGGTGTAAGGGGGGGAGGTTCTTTGGCATTTGATGAAGTCTCTCCATCTAATGTCTATAAAGATGTTCGATCATCCCAACCGAATTCTAATTATGGCACTGGATCCCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCGTTCTATCGGGACTGCAGTTGGTAGTAGAGCAGGTTCTGCAGGTATGGAAGCTACATCAGTTGCAGATTCAGAGGCAAAGCTTCTTCAGTCTTTTAGACACTGCATTGTGAAGCTTTTGAAGTTGGAAGGGTCTGACTGGTTGTTTAGACAGAATGACGGAGTGGATGAGGATCTAATAGATCGTGTGGCTGCCAGGGAGAAAATTCTTTATGATGCTGAAACAAGAGAGATTAATCGAACAGTTCACATGGGTGAATCTCCATACCCTTCTTCTGACAGGAAGTCTGCTTCTGCAAAAATGAATGATGTGAATCTCACCCATCTCATGGTTTCCTCGGTTCCTAATTGTGGGGAAGGTTGTATTTGGAGATCTGATCTAATAATAAGCTTTGGGGTCTGGTGTATTCATCGGATTCTTGATTTGTCCCTTATGGAAAGCCGGCCAGAGCTTTGGGGGAAATATACATATGTTCTGAATCGACTTCAGGGCATTATTGATGCTGCGTTCTCTAAGCCTCGTACTCCAATGTCTCCATGCTTCTGCCTTCAAATTGCAGCAGCCCAGCAGCAGAAGTCTAGTCCAACATTTTCGAATGGAATGTTACCCCCTGCTGCAAAACCAGCCAGGGGAAAATGCACGACAGCTGTAACGCTCCTGGACATAATCAAGGATGTGGAGATTGCAATTTCATGCCGAAAGGGCCGGACGGGCACAGCAGCTGGTGATGTGGCTTTTCCAAAGGGAAAAGAAAACCTGGCCTCTGTCCTCAAACGCTACAAGAGGCGGCTTTCCAATAAACCTGTTGGCACTAACGAGGGGCCTTCTGGTTCACGCAAGGGTACTGCAACATCTGCTCCTTATGCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1353

Amino Acids

146.91

Weight (kDa)

5.75

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 392 2.5e-83 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2020
Acc16I TGCGCA 2 cut(s) 2474, 2522
Acc36I ACCTGC 3 cut(s) 122, 3209, 3218
Acc65I GGTACC 1 cut(s) 1652
AccB1I GGYRCC 2 cut(s) 1652, 1760
AccB7I CCANNNNNTGG 3 cut(s) 1526, 3032, 3514
AccBSI CCGCTC 2 cut(s) 1968, 2503
AccI GTMKAC 4 cut(s) 2431, 2479, 2527, 2926
AccII CGCG 1 cut(s) 1716
AciI CCGC 8 cut(s) 116, 539, 1968, 2383, 2503, 2569, 2891, 3973
AcoI YGGCCR 2 cut(s) 2341, 3629
AcsI RAATTY 9 cut(s) 464, 1187, 1567, 1775, 2374, 2701, 2877, 3115, 3390
AcuI CTGAAG 4 cut(s) 1836, 1875, 3257, 3656
AcvI CACGTG 1 cut(s) 806
AcyI GRCGYC 1 cut(s) 2017
AfaI GTAC 9 cut(s) 148, 297, 1314, 1654, 1755, 2616, 2865, 3709, 4030
AhdI GACNNNNNGTC 2 cut(s) 944, 3469
AleI CACNNNNGTG 2 cut(s) 392, 3438
Alw21I GWGCWC 5 cut(s) 1046, 1390, 1901, 2427, 2971
Alw26I GTCTC 6 cut(s) 1438, 1451, 1815, 1943, 3076, 3723
Alw44I GTGCAC 1 cut(s) 2423
AlwNI CAGNNNCTG 5 cut(s) 41, 1052, 2281, 3224, 3908
AoxI GGCC 7 cut(s) 2002, 2336, 2341, 3629, 3889, 3945, 4007
ApaLI GTGCAC 1 cut(s) 2423
ApoI RAATTY 9 cut(s) 464, 1187, 1567, 1775, 2374, 2701, 2877, 3115, 3390
AseI ATTAAT 2 cut(s) 1154, 3420
Asp700I GAANNNNTTC 1 cut(s) 956
Asp718I GGTACC 1 cut(s) 1652
AspLEI GCGC 8 cut(s) 1946, 2312, 2412, 2460, 2475, 2508, 2523, 2556
AspS9I GGNCC 9 cut(s) 68, 325, 860, 970, 1919, 2002, 2337, 3889, 4007
AsuC2I CCSGG 1 cut(s) 2340
AsuII TTCGAA 1 cut(s) 3779
AsuNHI GCTAGC 1 cut(s) 11
AvaII GGWCC 5 cut(s) 68, 325, 860, 970, 1919
AxyI CCTNAGG 1 cut(s) 1425
BaeGI GKGCMC 3 cut(s) 1763, 2427, 3902
BaeI ACNNNNGTAYC 2 cut(s) 1545, 1578
BamHI GGATCC 2 cut(s) 2977, 3134
BanI GGYRCC 2 cut(s) 1652, 1760
BanII GRGCYC 2 cut(s) 1046, 1165
BarI GAAGNNNNNNTAC 6 cut(s) 2422, 2454, 2470, 2502, 2518, 2550
BbrPI CACGTG 1 cut(s) 806
BbsI GAAGAC 3 cut(s) 602, 803, 1808
Bbv12I GWGCWC 5 cut(s) 1046, 1390, 1901, 2427, 2971
BbvCI CCTCAGC 1 cut(s) 1053
BceAI ACGGC 2 cut(s) 1544, 1989
BciVI GTATCC 2 cut(s) 691, 3033
BclI TGATCA 1 cut(s) 1606
BcnI CCSGG 1 cut(s) 2340
BcoDI GTCTC 6 cut(s) 1438, 1451, 1815, 1943, 3076, 3723
BfaI CTAG 8 cut(s) 12, 621, 1991, 2013, 2024, 2958, 3147, 3765
BfmI CTRYAG 7 cut(s) 159, 222, 1698, 2214, 2731, 3201, 3225
BfoI RGCGCY 5 cut(s) 2313, 2413, 2461, 2509, 2557
BfuAI ACCTGC 3 cut(s) 122, 3209, 3218
BfuI GTATCC 2 cut(s) 691, 3033
BglI GCCNNNNNGGC 2 cut(s) 3888, 3897
BglII AGATCT 2 cut(s) 238, 3557
BmcAI AGTACT 1 cut(s) 1755
Bme18I GGWCC 5 cut(s) 68, 325, 860, 970, 1919
BmeRI GACNNNNNGTC 2 cut(s) 944, 3469
BmgT120I GGNCC 9 cut(s) 68, 325, 860, 970, 1919, 2002, 2337, 3889, 4007
BmrI ACTGGG 3 cut(s) 570, 1423, 1766
BmtI GCTAGC 1 cut(s) 15
BmuI ACTGGG 3 cut(s) 570, 1423, 1766
BpiI GAAGAC 3 cut(s) 602, 803, 1808
BpmI CTGGAG 1 cut(s) 946
Bpu10I CCTNAGC 3 cut(s) 778, 1045, 1053
Bpu14I TTCGAA 1 cut(s) 3779
BpuEI CTTGAG 2 cut(s) 1969, 2269
BpuMI CCSGG 1 cut(s) 2340
BsaAI YACGTR 1 cut(s) 806
BsaBI GATNNNNATC 3 cut(s) 3183, 3357, 3498
BsaHI GRCGYC 1 cut(s) 2017
BsaXI ACNNNNNCTCC 4 cut(s) 919, 949, 4027, 4057
Bse118I RCCGGY 1 cut(s) 3627
Bse21I CCTNAGG 1 cut(s) 1425
Bse3DI GCAATG 3 cut(s) 837, 1008, 2834
Bse8I GATNNNNATC 3 cut(s) 3183, 3357, 3498
BseJI GATNNNNATC 3 cut(s) 3183, 3357, 3498
BseMI GCAATG 3 cut(s) 837, 1008, 2834
BseMII CTCAG 8 cut(s) 260, 1044, 1059, 1190, 1905, 1954, 2577, 2913
BseRI GAGGAG 2 cut(s) 2860, 2973
BseSI GKGCMC 3 cut(s) 1763, 2427, 3902
BseYI CCCAGC 3 cut(s) 1295, 2592, 3750
BsgI GTGCAG 2 cut(s) 683, 2016
Bsh1236I CGCG 1 cut(s) 1716
BshFI GGCC 7 cut(s) 2004, 2338, 2343, 3631, 3891, 3947, 4009
BshNI GGYRCC 2 cut(s) 1652, 1760
BsiHKAI GWGCWC 5 cut(s) 1046, 1390, 1901, 2427, 2971
BsiSI CCGG 3 cut(s) 2340, 3628, 3892
BslFI GGGAC 7 cut(s) 392, 820, 1544, 1703, 2064, 3211, 3598
BsmAI GTCTC 6 cut(s) 1438, 1451, 1815, 1943, 3076, 3723
BsmFI GGGAC 7 cut(s) 392, 820, 1544, 1703, 2064, 3211, 3598
BsmI GAATGC 2 cut(s) 791, 2893
BsnI GGCC 7 cut(s) 2004, 2338, 2343, 3631, 3891, 3947, 4009
Bsp119I TTCGAA 1 cut(s) 3779
Bsp1286I GDGCHC 8 cut(s) 1046, 1165, 1390, 1763, 1901, 2427, 2971, 3902
Bsp19I CCATGG 2 cut(s) 2068, 2754
BspACI CCGC 8 cut(s) 116, 539, 1968, 2383, 2503, 2569, 2891, 3973
BspANI GGCC 7 cut(s) 2004, 2338, 2343, 3631, 3891, 3947, 4009
BspCNI CTCAG 8 cut(s) 259, 1045, 1058, 1189, 1906, 1953, 2578, 2912
BspFNI CGCG 1 cut(s) 1716
BspMAI CTGCAG 3 cut(s) 163, 3205, 3229
BspMI ACCTGC 3 cut(s) 122, 3209, 3218
BspOI GCTAGC 1 cut(s) 15
BspT104I TTCGAA 1 cut(s) 3779
BspT107I GGYRCC 2 cut(s) 1652, 1760
BsrBI CCGCTC 2 cut(s) 1968, 2503
BsrDI GCAATG 3 cut(s) 837, 1008, 2834
BsrFI RCCGGY 1 cut(s) 3627
BssAI RCCGGY 1 cut(s) 3627
BssNAI GTATAC 3 cut(s) 2432, 2480, 2528
BssNI GRCGYC 1 cut(s) 2017
BssT1I CCWWGG 2 cut(s) 2068, 2754
Bst1107I GTATAC 3 cut(s) 2432, 2480, 2528
Bst6I CTCTTC 2 cut(s) 229, 255
BstACI GRCGYC 1 cut(s) 2017
BstAPI GCANNNNNTGC 5 cut(s) 788, 2281, 2767, 3224, 4040
BstBAI YACGTR 1 cut(s) 806
BstBI TTCGAA 1 cut(s) 3779
BstC8I GCNNGC 3 cut(s) 13, 2607, 3629
BstDSI CCRYGG 3 cut(s) 2068, 2754, 2972
BstFNI CGCG 1 cut(s) 1716
BstH2I RGCGCY 5 cut(s) 2313, 2413, 2461, 2509, 2557
BstHHI GCGC 8 cut(s) 1946, 2312, 2412, 2460, 2475, 2508, 2523, 2556
BstMAI GTCTC 6 cut(s) 1438, 1451, 1815, 1943, 3076, 3723
BstNSI RCATGY 3 cut(s) 282, 739, 2350
BstSFI CTRYAG 7 cut(s) 159, 222, 1698, 2214, 2731, 3201, 3225
BstSLI GKGCMC 3 cut(s) 1763, 2427, 3902
BstUI CGCG 1 cut(s) 1716
BstV2I GAAGAC 3 cut(s) 602, 803, 1808
BstX2I RGATCY 8 cut(s) 238, 2107, 2228, 2321, 2977, 3134, 3358, 3557
BstXI CCANNNNNNTGG 2 cut(s) 445, 3639
BstYI RGATCY 8 cut(s) 238, 2107, 2228, 2321, 2977, 3134, 3358, 3557
BstZ17I GTATAC 3 cut(s) 2432, 2480, 2528
Bsu36I CCTNAGG 1 cut(s) 1425
BsuI GTATCC 2 cut(s) 691, 3033
BsuRI GGCC 7 cut(s) 2004, 2338, 2343, 3631, 3891, 3947, 4009
BtgI CCRYGG 3 cut(s) 2068, 2754, 2972
BtsI GCAGTG 5 cut(s) 168, 1323, 2181, 2392, 3286
BveI ACCTGC 3 cut(s) 122, 3209, 3218
Cac8I GCNNGC 3 cut(s) 13, 2607, 3629
CaiI CAGNNNCTG 5 cut(s) 41, 1052, 2281, 3224, 3908
CfoI GCGC 8 cut(s) 1946, 2312, 2412, 2460, 2475, 2508, 2523, 2556
Cfr10I RCCGGY 1 cut(s) 3627
Cfr13I GGNCC 9 cut(s) 68, 325, 860, 970, 1919, 2002, 2337, 3889, 4007
CseI GACGC 3 cut(s) 1541, 1705, 4041
CsiI ACCWGGT 2 cut(s) 34, 1401
Csp6I GTAC 9 cut(s) 147, 296, 1313, 1653, 1754, 2615, 2864, 3708, 4029
CviQI GTAC 9 cut(s) 147, 296, 1313, 1653, 1754, 2615, 2864, 3708, 4029
DriI GACNNNNNGTC 2 cut(s) 944, 3469
EaeI YGGCCR 2 cut(s) 2341, 3629
Eam1104I CTCTTC 2 cut(s) 229, 255
Eam1105I GACNNNNNGTC 2 cut(s) 944, 3469
EarI CTCTTC 2 cut(s) 229, 255
Ecl136II GAGCTC 1 cut(s) 1044
Eco130I CCWWGG 2 cut(s) 2068, 2754
Eco24I GRGCYC 2 cut(s) 1046, 1165
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 5 cut(s) 68, 325, 860, 970, 1919
Eco53kI GAGCTC 1 cut(s) 1044
Eco57I CTGAAG 4 cut(s) 1836, 1875, 3257, 3656
Eco72I CACGTG 1 cut(s) 806
Eco81I CCTNAGG 1 cut(s) 1425
EcoICRI GAGCTC 1 cut(s) 1044
EcoO109I RGGNCCY 1 cut(s) 4007
EcoRI GAATTC 1 cut(s) 3115
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 2 cut(s) 2068, 2754
EcoT22I ATGCAT 2 cut(s) 284, 793
EcoT38I GRGCYC 2 cut(s) 1046, 1165
ErhI CCWWGG 2 cut(s) 2068, 2754
FalI AAGNNNNNCTT 2 cut(s) 2308, 2340
FaqI GGGAC 7 cut(s) 392, 820, 1544, 1703, 2064, 3211, 3598
FauNDI CATATG 2 cut(s) 2305, 3655
FbaI TGATCA 1 cut(s) 1606
FblI GTMKAC 4 cut(s) 2431, 2479, 2527, 2926
FriOI GRGCYC 2 cut(s) 1046, 1165
FspBI CTAG 8 cut(s) 12, 621, 1991, 2013, 2024, 2958, 3147, 3765
FspI TGCGCA 2 cut(s) 2474, 2522
GlaI GCGC 8 cut(s) 1945, 2311, 2411, 2459, 2474, 2507, 2522, 2555
GsaI CCCAGC 3 cut(s) 1299, 2596, 3754
GsuI CTGGAG 1 cut(s) 946
HaeII RGCGCY 5 cut(s) 2313, 2413, 2461, 2509, 2557
HaeIII GGCC 7 cut(s) 2004, 2338, 2343, 3631, 3891, 3947, 4009
HapII CCGG 3 cut(s) 2340, 3628, 3892
HgaI GACGC 3 cut(s) 1541, 1705, 4041
HhaI GCGC 8 cut(s) 1946, 2312, 2412, 2460, 2475, 2508, 2523, 2556
Hin1I GRCGYC 1 cut(s) 2017
Hin6I GCGC 8 cut(s) 1944, 2310, 2410, 2458, 2473, 2506, 2521, 2554
HinP1I GCGC 8 cut(s) 1944, 2310, 2410, 2458, 2473, 2506, 2521, 2554
HincII GTYRAC 3 cut(s) 536, 1908, 2021
HindII GTYRAC 3 cut(s) 536, 1908, 2021
HindIII AAGCTT 3 cut(s) 3266, 3299, 3574
HpaI GTTAAC 1 cut(s) 1908
HpaII CCGG 3 cut(s) 2340, 3628, 3892
HpyAV CCTTC 9 cut(s) 889, 983, 1811, 1952, 3308, 3465, 3536, 3743, 4020
HpyCH4IV ACGT 4 cut(s) 486, 805, 1904, 2017
HpySE526I ACGT 4 cut(s) 486, 805, 1904, 2017
Hsp92I GRCGYC 1 cut(s) 2017
HspAI GCGC 8 cut(s) 1944, 2310, 2410, 2458, 2473, 2506, 2521, 2554
KpnI GGTACC 1 cut(s) 1656
KroI GCCGGC 1 cut(s) 3627
KroNI GCCGGC 1 cut(s) 3629
Ksp22I TGATCA 1 cut(s) 1606
KspAI GTTAAC 1 cut(s) 1908
MabI ACCWGGT 2 cut(s) 34, 1401
MaeI CTAG 8 cut(s) 12, 621, 1991, 2013, 2024, 2958, 3147, 3765
MaeII ACGT 4 cut(s) 486, 805, 1904, 2017
MaeIII GTNAC 8 cut(s) 197, 226, 1747, 1952, 2666, 2688, 3789, 3835
MbiI CCGCTC 2 cut(s) 1968, 2503
MflI RGATCY 8 cut(s) 238, 2107, 2228, 2321, 2977, 3134, 3358, 3557
MhlI GDGCHC 8 cut(s) 1046, 1165, 1390, 1763, 1901, 2427, 2971, 3902
MlyI GAGTC 8 cut(s) 14, 616, 937, 1798, 1945, 2255, 2306, 2696
MmeI TCCRAC 5 cut(s) 46, 70, 303, 3291, 3794
Mph1103I ATGCAT 2 cut(s) 284, 793
MroNI GCCGGC 1 cut(s) 3627
MroXI GAANNNNTTC 1 cut(s) 956
MslI CAYNNNNRTG 4 cut(s) 392, 1488, 2073, 3438
MspA1I CMGCKG 2 cut(s) 3833, 3908
MspI CCGG 3 cut(s) 2340, 3628, 3892
Mva1269I GAATGC 2 cut(s) 791, 2893
MvnI CGCG 1 cut(s) 1716
NaeI GCCGGC 1 cut(s) 3629
NciI CCSGG 1 cut(s) 2340
NcoI CCATGG 2 cut(s) 2068, 2754
NdeI CATATG 2 cut(s) 2305, 3655
NgoMIV GCCGGC 1 cut(s) 3627
NheI GCTAGC 1 cut(s) 11
NmuCI GTSAC 4 cut(s) 197, 226, 1952, 2688
NsbI TGCGCA 2 cut(s) 2474, 2522
NsiI ATGCAT 2 cut(s) 284, 793
NspI RCATGY 3 cut(s) 282, 739, 2350
NspV TTCGAA 1 cut(s) 3779
OliI CACNNNNGTG 2 cut(s) 392, 3438
PctI GAATGC 2 cut(s) 791, 2893
PdiI GCCGGC 1 cut(s) 3629
PdmI GAANNNNTTC 1 cut(s) 956
PflFI GACNNNGTC 1 cut(s) 2829
PflMI CCANNNNNTGG 3 cut(s) 1526, 3032, 3514
PfoI TCCNGGA 3 cut(s) 444, 1921, 3843
PleI GAGTC 8 cut(s) 13, 615, 936, 1798, 1944, 2255, 2305, 2695
PmaCI CACGTG 1 cut(s) 806
PmlI CACGTG 1 cut(s) 806
PpsI GAGTC 8 cut(s) 13, 615, 936, 1798, 1944, 2255, 2305, 2695
Ppu21I YACGTR 1 cut(s) 806
PshBI ATTAAT 2 cut(s) 1154, 3420
Psp124BI GAGCTC 1 cut(s) 1046
PspCI CACGTG 1 cut(s) 806
PspFI CCCAGC 3 cut(s) 1295, 2592, 3750
PspPI GGNCC 9 cut(s) 68, 325, 860, 970, 1919, 2002, 2337, 3889, 4007
PsrI GAACNNNNNNTAC 2 cut(s) 3205, 3237
PstI CTGCAG 3 cut(s) 163, 3205, 3229
PstNI CAGNNNCTG 5 cut(s) 41, 1052, 2281, 3224, 3908
PsuI RGATCY 8 cut(s) 238, 2107, 2228, 2321, 2977, 3134, 3358, 3557
PsyI GACNNNGTC 1 cut(s) 2829
PvuII CAGCTG 2 cut(s) 3833, 3908
RsaI GTAC 9 cut(s) 148, 297, 1314, 1654, 1755, 2616, 2865, 3709, 4030
RsaNI GTAC 9 cut(s) 147, 296, 1313, 1653, 1754, 2615, 2864, 3708, 4029
RseI CAYNNNNRTG 4 cut(s) 392, 1488, 2073, 3438
SacI GAGCTC 1 cut(s) 1046
Sau96I GGNCC 9 cut(s) 68, 325, 860, 970, 1919, 2002, 2337, 3889, 4007
ScaI AGTACT 1 cut(s) 1755
SchI GAGTC 8 cut(s) 14, 616, 937, 1798, 1945, 2255, 2306, 2696
SduI GDGCHC 8 cut(s) 1046, 1165, 1390, 1763, 1901, 2427, 2971, 3902
SexAI ACCWGGT 2 cut(s) 34, 1401
SfcI CTRYAG 7 cut(s) 159, 222, 1698, 2214, 2731, 3201, 3225
SfuI TTCGAA 1 cut(s) 3779
SinI GGWCC 5 cut(s) 68, 325, 860, 970, 1919
SmiMI CAYNNNNRTG 4 cut(s) 392, 1488, 2073, 3438
SmlI CTYRAG 2 cut(s) 1984, 2248
SmoI CTYRAG 2 cut(s) 1984, 2248
SsiI CCGC 8 cut(s) 116, 539, 1968, 2383, 2503, 2569, 2891, 3973
SspMI CTAG 8 cut(s) 12, 621, 1991, 2013, 2024, 2958, 3147, 3765
SstI GAGCTC 1 cut(s) 1046
StyI CCWWGG 2 cut(s) 2068, 2754
TaiI ACGT 4 cut(s) 489, 808, 1907, 2020
TaqI TCGA 7 cut(s) 1390, 2037, 2824, 3100, 3424, 3667, 3779
TatI WGTACW 3 cut(s) 1312, 1753, 2614
TauI GCSGC 4 cut(s) 119, 2385, 2506, 3976
TseFI GTSAC 4 cut(s) 197, 226, 1952, 2688
Tsp45I GTSAC 4 cut(s) 197, 226, 1952, 2688
TspGWI ACGGA 1 cut(s) 3360
Tth111I GACNNNGTC 1 cut(s) 2829
Van91I CCANNNNNTGG 3 cut(s) 1526, 3032, 3514
VneI GTGCAC 1 cut(s) 2423
VpaK11BI GGWCC 5 cut(s) 68, 325, 860, 970, 1919
VspI ATTAAT 2 cut(s) 1154, 3420
XapI RAATTY 9 cut(s) 464, 1187, 1567, 1775, 2374, 2701, 2877, 3115, 3390
XbaI TCTAGA 1 cut(s) 3146
XceI RCATGY 3 cut(s) 282, 739, 2350
XmiI GTMKAC 4 cut(s) 2431, 2479, 2527, 2926
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 8 cut(s) 12, 621, 1991, 2013, 2024, 2958, 3147, 3765
ZraI GACGTC 1 cut(s) 2018
ZrmI AGTACT 1 cut(s) 1755
Zsp2I ATGCAT 2 cut(s) 284, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.