Rw3G014480

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002662
Physical Location & Seq
Forward (+)
14611265 .. 14619417
8153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G014480.1

Sequence Viewer

Length: 3945 bp
ATGTCCAAGAGTCTGATAGCTCATATGGAATCTGCTGGCTCTAACGCTAACAATATGCCAGGCGTTGTCCATCAGTTGCTTCCTGTTGTTGGACCCATGCTTCTGATTGCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGTTGAAGCAGGTTCCCGTTATGGAACTGATCTGGCTGCAGTAATGCTTATATTCAATTTGGCTGCTATTTTATGTCACTATCTGTCAGCACGGATTGCTGTAGTCACTGGAAGAGATCTTGCTCAGGTTTGCAGTGAGGAGTATGACAAGGCTACGTGCATATTCTTAGGAGTACAAACAGAGATGTCGGTGATTGTGTTGGACCTTACCATGATCCTCGGTATCGCACATGCACTTAATCTTCTGTTTGGGTGGGACTTGTTCACATGTGTGTTTTTGACTGCTGCTAATGCTGTTTTATACCCTCTTTTTTCCACCCTCCTGGAGACTTGCAAGGCGAAATTCCTTTGCGTATGCATATACATTGCAGGATTTCTACTGCTTTCCTTTGTTCTTGGAGTATTTATCAGTCAACCACAAGTGCCACTTTCCATGACTGGGATGTTAACAAAACTGAGTGGGGAAAGTGCTTTTTCACTGATGAGTCTTCTTGGAGCAAGTATAATGCCCCACAGTTTTTATCTTCATTCTTCTATTGTGCAGCAGCATCAGCAACAACCAACTGTTTCTAAGGATGCCTTGTGTCAGAACCATTTTGTTGCCATCTTCTGCACCTTTAGTGGTATTTATCTGGTGAATTATGCCCTCATGACCTTAGCAGCAAATGTATTCTACACTTCACGTGGTTTGCTCACATTTCAGGATGCAATGTCCCTAATGGAACAGGTCTTTTGGGGTCCAATAGTACCTGTTGCCTTCTTGCTAGTTCTCTTTTTATCAAATCAAATCACAACATTAAGCTGGAGTCTGGGGGGACAAGTAGTCTTGAATGATTTTTTAAAACTAGACCTTCCTGGTTGGCTTCACTGTGCTACAATCAGAATCATTGCCATTGTTCCTGCTCTGTATTTTGTTTGGAGCTCAGGAGCTGAGGGGATGTACCAACTGCTTATATCTACACAGGTATTGGCAGCCTTGCTACTGCCGTCTTCTGTGATCCCTCTTTTTCGTGTTGCTGCTTCAAGACAAATAATGGGAGCCCATAAGATCTCTCAGTTCGTTGAATTCTTGGCCCTCATTACACTTATTGGGATGCTTGGATTAAAACTTGTCTTTGTAGTGGAAATGATTTTTGGGAATAGTGATTGGGTGGATAATTTGAGATGGGATGCTGGGAGTAGTATGTCTGCACTTCTCATCACTGCTTCTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACACCGTTAAAATCTGCAAGTGCTCGATTAGAGAACCAGGTGTGGAACTGGGATATGCCTAAGGGTGTATCTGAGCCATTTAGAAATAAGGAGGAGATTGATATAGCTGAACCTAATTATCATAGAGATGCAAGTGTTCAGAAGCATGAACCATCACCATCTTCTGGGAAGGCTTTGGATAGAGACTCGGATACAGCGGTTGCAAATTTTGATTTTGTTCTGCCCGAAACTCTCTTGGAGCCTGATCAGGAGCTTCAATCAACTACCTCAGAGGAAAATGGTTCTCTTAGTACCTTTCCTCGCTCTGCCAAATGCAGTAAGGAGGAACCCACATCTGTGGTGGAGTCAATTTGCCTCCCAACTGTGGCTAGTGAGGTTTCTGATGTTACATCACTGGGCACCAGTACTGTGAAAGTTGGATCAACAGAGCCAGTTGAGAAGATTCTTGGAGTTGAAGGAGACTTACCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAACCTGAAGATTCCTTGAAAGAGGTTTCTGGGGGCACCACTTCATTGACATCTGAAGGTCCAGGATCATTCAGGAGTCTCAGTGGAAAAGGTGATGAAGGGGGGAGTGGTGCTGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCTAGACGTCAACTGGCTGCAGTACTCGATGAGTTTTGGGGACAACTGTATGATTTCCATGGTAATGTAATTCAAGAAGCAAAGGCTAGGAAACTGGATCTGTTGTTGGGGTCAGATTCAAAGGCTTCTTCTGCTGCTTCCTCCGCTTCCTCTGTGCTGAAAGATGATACCACTGCAAAGGAAGTTTCTGGATACTTTCCATCTGTAGGAGGCAGAGGATCTGATCCTTTAATCAACTCAAGTTTATATGACTCCGTAAATCAGCCAAGGCTGCAAAACAGTTTAGAGTCATCATATGGTGCTCAAAGGGGATCTTCCTCATTATGGTCTGGCCACATACAGTTGCTGGATGCGTATGTACAAAATTCAACCTGCAGTATTATTGACTCGGGTGAGAGGCGCTATTCAAGTGTCCGCAGCATACCATCTGCTGAGAGCTGGGATTACCAGCCAGCCACAGTACACGGTTATCAGATTTCATCGTATCTTAATCGTAATGACAGAAGTTCTGGTAACTTGAATGGTCAAATGGAATCACCAGCCCTAAATTCTGCTTCTTCATTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGCTCTGGACAGGCCTCCAGTTTTCAGAACCTTACGGTATCTCGACATAGTCCGTTGCAATCTGAAAGACCATATTATGATGTACACTCTTCTGGACTTTCTGAGAATGTGGTAAATTCAGCCAATGCAAAGAAATACCACAGTTTACCTGACATTCACCGCGATCTCTACATGTCTAATAAAAGTGCTCAACGGGATGCTCCCCCTGGGTTTGGGAAAACTAATTATGAATCATCCTTGTATCCAAAATCTGTTGCACGGACAGGAGGTCCTTTGGCATTTGATGAACTCTCTCCATCAAAAGTCTATAGAGATGTTCTATCATCCCAACAGAATTCTAATTTTGGCACTGGATCTCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCGTTCTATTGGGACTGCAGTTGGTAGTAGAGCAGGTTCTGCAGGTCAGGAAGCTATGTCAGTTGCAGATTCAGAGGCGAAGCTTCTTCAGTCTTTTAGACACTGCATTGTGAAGCTTTTGAAGTTGGAAGGGTCTGACTGGTTGTTTAGACAGAATGATGGAGTGGATGAGGATCTAATAGATCGTGTGGCTGCCAGGGAGAAAATTCTTTATGAAGCTGAAACTAGAGAGATTAATCGAACAGTTCACATGGGTGAATCTCAGTACACTTCTGACAGGAAGTCAACTTCTGCAATAAAAATGAGTGATGCAAATCTCACCCATCTCATGGTTTCCTCAGTTCCTAATTGTGGGGAAGGTTGTATCTGGAGATCTGATCTAATAATAAGCTTTGGGGTCTGGTGTATCCATCGGATTCTTGATTTGTCCCTTATGGAAAGCCGGCCAGAGCTCTGGGGGAAATATACTTACGTTCTCAATCGACTTCAGGGGATTATTGATTCTGCGTTTTCGAAGCCTCGTTCTCCAATGTCTCCATGCTTCTGCCTTCAAATTGCAGCAGCACAGCAGCAGAAGTCCAGTCCAACATTTTCGAATGGAATGTTACCCCCTGCTGCAAAACCAGCCAGGGGAAAATGCACTACAGCTGCAACGCTCCTGGACATAATCAAGGATGTGGAGATTGCAATCTCTTGTCGAAAGGGCCGAACGGGGACGGCAGCTGGTGATGTGGCTTTCCCGAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGGCGATTATCCAACAAACCTGTTTGCACTCAGGAGGGGCCTTCCGGTTCACGGAAGGGTGCTCCAACATCTGCTCCTTACGGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1314

Amino Acids

142.27

Weight (kDa)

5.83

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 46 - 400 9.9e-84 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2050
Acc36I ACCTGC 4 cut(s) 146, 2420, 3095, 3104
AccB1I GGYRCC 2 cut(s) 1787, 1925
AccB7I CCANNNNNTGG 2 cut(s) 1553, 3400
AccII CGCG 1 cut(s) 2829
AciI CCGC 6 cut(s) 140, 1586, 2184, 2455, 2827, 3856
AcoI YGGCCR 2 cut(s) 2371, 3515
AcsI RAATTY 8 cut(s) 488, 1211, 1594, 2404, 2587, 2782, 3001, 3276
AcuI CTGAAG 4 cut(s) 1917, 1965, 3143, 3542
AcvI CACGTG 1 cut(s) 830
AcyI GRCGYC 1 cut(s) 2047
AflIII ACRYGT 2 cut(s) 413, 2838
AhdI GACNNNNNGTC 3 cut(s) 968, 2934, 3352
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
AleI CACNNNNGTG 3 cut(s) 416, 1724, 3324
Alw21I GWGCWC 6 cut(s) 1070, 1414, 2344, 2857, 3525, 3919
Alw26I GTCTC 5 cut(s) 467, 1566, 1842, 1973, 3609
AlwNI CAGNNNCTG 3 cut(s) 1076, 2386, 3110
Ama87I CYCGRG 1 cut(s) 2428
AoxI GGCC 6 cut(s) 1218, 2371, 2679, 3515, 3775, 3893
ApoI RAATTY 8 cut(s) 488, 1211, 1594, 2404, 2587, 2782, 3001, 3276
AseI ATTAAT 1 cut(s) 3306
Asp700I GAANNNNTTC 1 cut(s) 2011
AspLEI GCGC 1 cut(s) 2442
AspS9I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 2936, 3775, 3893
AsuII TTCGAA 2 cut(s) 3584, 3665
AvaI CYCGRG 1 cut(s) 2428
AvaII GGWCC 5 cut(s) 92, 349, 884, 1949, 2936
AxyI CCTNAGG 1 cut(s) 1449
BaeGI GKGCMC 2 cut(s) 1790, 1928
BaeI ACNNNNGTAYC 2 cut(s) 1572, 1605
BalI TGGCCA 1 cut(s) 2373
BanI GGYRCC 2 cut(s) 1787, 1925
BanII GRGCYC 3 cut(s) 1070, 1189, 3525
BarI GAAGNNNNNNTAC 2 cut(s) 1836, 1868
BbrPI CACGTG 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 626, 1128
Bbv12I GWGCWC 6 cut(s) 1070, 1414, 2344, 2857, 3525, 3919
BbvCI CCTCAGC 1 cut(s) 1077
BceAI ACGGC 2 cut(s) 1117, 3804
BciVI GTATCC 4 cut(s) 1573, 2225, 2919, 3488
BclI TGATCA 1 cut(s) 1633
BcoDI GTCTC 5 cut(s) 467, 1566, 1842, 1973, 3609
BfaI CTAG 7 cut(s) 911, 992, 1758, 2043, 2127, 3033, 3297
BfoI RGCGCY 1 cut(s) 2443
BfuAI ACCTGC 4 cut(s) 146, 2420, 3095, 3104
BfuI GTATCC 4 cut(s) 1573, 2225, 2919, 3488
BglII AGATCT 3 cut(s) 262, 1194, 3443
BmcAI AGTACT 2 cut(s) 1795, 2064
Bme18I GGWCC 5 cut(s) 92, 349, 884, 1949, 2936
BmeRI GACNNNNNGTC 3 cut(s) 968, 2934, 3352
BmeT110I CYCGRG 1 cut(s) 2428
BmgT120I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 2936, 3775, 3893
BmrI ACTGGG 3 cut(s) 594, 1447, 1793
BmuI ACTGGG 3 cut(s) 594, 1447, 1793
BpiI GAAGAC 2 cut(s) 626, 1128
BpmI CTGGAG 4 cut(s) 491, 970, 2668, 3460
Bpu10I CCTNAGC 4 cut(s) 270, 802, 1069, 1077
Bpu14I TTCGAA 2 cut(s) 3584, 3665
BpuEI CTTGAG 1 cut(s) 2263
BsaAI YACGTR 2 cut(s) 303, 830
BsaBI GATNNNNATC 4 cut(s) 3069, 3243, 3384, 3758
BsaHI GRCGYC 1 cut(s) 2047
BsaWI WCCGGW 1 cut(s) 3899
BsaXI ACNNNNNCTCC 4 cut(s) 1957, 1987, 3913, 3943
Bse118I RCCGGY 1 cut(s) 3513
Bse21I CCTNAGG 1 cut(s) 1449
Bse3DI GCAATG 3 cut(s) 510, 861, 1032
Bse8I GATNNNNATC 4 cut(s) 3069, 3243, 3384, 3758
BseJI GATNNNNATC 4 cut(s) 3069, 3243, 3384, 3758
BseMI GCAATG 3 cut(s) 510, 861, 1032
BseRI GAGGAG 2 cut(s) 299, 1496
BseSI GKGCMC 2 cut(s) 1790, 1928
BseYI CCCAGC 2 cut(s) 1319, 2478
BsgI GTGCAG 3 cut(s) 707, 742, 1320
Bsh1236I CGCG 1 cut(s) 2829
BshFI GGCC 6 cut(s) 1220, 2373, 2681, 3517, 3777, 3895
BshNI GGYRCC 2 cut(s) 1787, 1925
BsiHKAI GWGCWC 6 cut(s) 1070, 1414, 2344, 2857, 3525, 3919
BsiHKCI CYCGRG 1 cut(s) 2428
BsiSI CCGG 2 cut(s) 3514, 3900
BslFI GGGAC 7 cut(s) 416, 844, 975, 2094, 3097, 3484, 3799
BsmAI GTCTC 5 cut(s) 467, 1566, 1842, 1973, 3609
BsmFI GGGAC 7 cut(s) 416, 844, 975, 2094, 3097, 3484, 3799
BsnI GGCC 6 cut(s) 1220, 2373, 2681, 3517, 3777, 3895
BsoBI CYCGRG 1 cut(s) 2428
Bsp119I TTCGAA 2 cut(s) 3584, 3665
Bsp1286I GDGCHC 9 cut(s) 1070, 1189, 1414, 1790, 1928, 2344, 2857, 3525, 3919
Bsp1407I TGTACA 2 cut(s) 2398, 2749
Bsp19I CCATGG 2 cut(s) 2098, 2640
BspACI CCGC 6 cut(s) 140, 1586, 2184, 2455, 2827, 3856
BspANI GGCC 6 cut(s) 1220, 2373, 2681, 3517, 3777, 3895
BspFNI CGCG 1 cut(s) 2829
BspHI TCATGA 1 cut(s) 795
BspMAI CTGCAG 5 cut(s) 187, 2062, 2417, 3091, 3115
BspMI ACCTGC 4 cut(s) 146, 2420, 3095, 3104
BspT104I TTCGAA 2 cut(s) 3584, 3665
BspT107I GGYRCC 2 cut(s) 1787, 1925
BsrDI GCAATG 3 cut(s) 510, 861, 1032
BsrFI RCCGGY 1 cut(s) 3513
BsrGI TGTACA 2 cut(s) 2398, 2749
BssAI RCCGGY 1 cut(s) 3513
BssNI GRCGYC 1 cut(s) 2047
BssT1I CCWWGG 3 cut(s) 2098, 2306, 2640
Bst6I CTCTTC 2 cut(s) 253, 2761
BstACI GRCGYC 1 cut(s) 2047
BstAPI GCANNNNNTGC 3 cut(s) 242, 2653, 3110
BstAUI TGTACA 2 cut(s) 2398, 2749
BstBAI YACGTR 2 cut(s) 303, 830
BstBI TTCGAA 2 cut(s) 3584, 3665
BstC8I GCNNGC 5 cut(s) 37, 1383, 2037, 2493, 3515
BstDSI CCRYGG 2 cut(s) 2098, 2640
BstFNI CGCG 1 cut(s) 2829
BstH2I RGCGCY 1 cut(s) 2443
BstHHI GCGC 1 cut(s) 2442
BstMAI GTCTC 5 cut(s) 467, 1566, 1842, 1973, 3609
BstNSI RCATGY 3 cut(s) 380, 417, 2842
BstSLI GKGCMC 2 cut(s) 1790, 1928
BstUI CGCG 1 cut(s) 2829
BstV2I GAAGAC 2 cut(s) 626, 1128
BstX2I RGATCY 8 cut(s) 262, 1194, 2137, 2258, 2351, 3020, 3244, 3443
BstXI CCANNNNNNTGG 3 cut(s) 469, 1726, 3525
BstYI RGATCY 8 cut(s) 262, 1194, 2137, 2258, 2351, 3020, 3244, 3443
Bsu36I CCTNAGG 1 cut(s) 1449
BsuI GTATCC 4 cut(s) 1573, 2225, 2919, 3488
BsuRI GGCC 6 cut(s) 1220, 2373, 2681, 3517, 3777, 3895
BtgI CCRYGG 2 cut(s) 2098, 2640
BtsI GCAGTG 4 cut(s) 286, 1347, 2211, 3172
BveI ACCTGC 4 cut(s) 146, 2420, 3095, 3104
Cac8I GCNNGC 5 cut(s) 37, 1383, 2037, 2493, 3515
CaiI CAGNNNCTG 3 cut(s) 1076, 2386, 3110
CciI TCATGA 1 cut(s) 795
CfoI GCGC 1 cut(s) 2442
Cfr10I RCCGGY 1 cut(s) 3513
Cfr13I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 2936, 3775, 3893
CsiI ACCWGGT 1 cut(s) 1425
DraI TTTAAA 1 cut(s) 987
DriI GACNNNNNGTC 3 cut(s) 968, 2934, 3352
EaeI YGGCCR 2 cut(s) 2371, 3515
Eam1104I CTCTTC 2 cut(s) 253, 2761
Eam1105I GACNNNNNGTC 3 cut(s) 968, 2934, 3352
EarI CTCTTC 2 cut(s) 253, 2761
Ecl136II GAGCTC 2 cut(s) 1068, 3523
Eco130I CCWWGG 3 cut(s) 2098, 2306, 2640
Eco147I AGGCCT 1 cut(s) 2681
Eco24I GRGCYC 3 cut(s) 1070, 1189, 3525
Eco32I GATATC 1 cut(s) 119
Eco47I GGWCC 5 cut(s) 92, 349, 884, 1949, 2936
Eco53kI GAGCTC 2 cut(s) 1068, 3523
Eco57I CTGAAG 4 cut(s) 1917, 1965, 3143, 3542
Eco72I CACGTG 1 cut(s) 830
Eco81I CCTNAGG 1 cut(s) 1449
Eco88I CYCGRG 1 cut(s) 2428
EcoICRI GAGCTC 2 cut(s) 1068, 3523
EcoO109I RGGNCCY 2 cut(s) 2936, 3893
EcoRI GAATTC 2 cut(s) 1211, 3001
EcoRV GATATC 1 cut(s) 119
EcoT14I CCWWGG 3 cut(s) 2098, 2306, 2640
EcoT22I ATGCAT 1 cut(s) 506
EcoT38I GRGCYC 3 cut(s) 1070, 1189, 3525
ErhI CCWWGG 3 cut(s) 2098, 2306, 2640
FalI AAGNNNNNCTT 6 cut(s) 558, 590, 710, 742, 2338, 2370
FaqI GGGAC 7 cut(s) 416, 844, 975, 2094, 3097, 3484, 3799
FauNDI CATATG 2 cut(s) 24, 2335
FbaI TGATCA 1 cut(s) 1633
FriOI GRGCYC 3 cut(s) 1070, 1189, 3525
FspBI CTAG 7 cut(s) 911, 992, 1758, 2043, 2127, 3033, 3297
GlaI GCGC 1 cut(s) 2441
GsaI CCCAGC 2 cut(s) 1323, 2482
GsuI CTGGAG 4 cut(s) 491, 970, 2668, 3460
HaeII RGCGCY 1 cut(s) 2443
HaeIII GGCC 6 cut(s) 1220, 2373, 2681, 3517, 3777, 3895
HapII CCGG 2 cut(s) 3514, 3900
HhaI GCGC 1 cut(s) 2442
Hin1I GRCGYC 1 cut(s) 2047
Hin6I GCGC 1 cut(s) 2440
HinP1I GCGC 1 cut(s) 2440
HincII GTYRAC 4 cut(s) 560, 594, 2051, 3357
HindII GTYRAC 4 cut(s) 560, 594, 2051, 3357
HindIII AAGCTT 3 cut(s) 3152, 3185, 3460
HpaI GTTAAC 1 cut(s) 594
HpaII CCGG 2 cut(s) 3514, 3900
HpyCH4IV ACGT 4 cut(s) 302, 829, 2047, 3543
HpySE526I ACGT 4 cut(s) 302, 829, 2047, 3543
Hsp92I GRCGYC 1 cut(s) 2047
HspAI GCGC 1 cut(s) 2440
KroI GCCGGC 1 cut(s) 3513
KroNI GCCGGC 1 cut(s) 3515
Ksp22I TGATCA 1 cut(s) 1633
KspAI GTTAAC 1 cut(s) 594
MabI ACCWGGT 1 cut(s) 1425
MaeI CTAG 7 cut(s) 911, 992, 1758, 2043, 2127, 3033, 3297
MaeII ACGT 4 cut(s) 302, 829, 2047, 3543
MaeIII GTNAC 5 cut(s) 221, 250, 1774, 2552, 3675
MflI RGATCY 8 cut(s) 262, 1194, 2137, 2258, 2351, 3020, 3244, 3443
MhlI GDGCHC 9 cut(s) 1070, 1189, 1414, 1790, 1928, 2344, 2857, 3525, 3919
MlsI TGGCCA 1 cut(s) 2373
MluNI TGGCCA 1 cut(s) 2373
MlyI GAGTC 9 cut(s) 19, 640, 961, 1568, 1742, 1975, 2285, 2336, 2420
MmeI TCCRAC 8 cut(s) 70, 94, 327, 1786, 3177, 3680, 3891, 3944
Mox20I TGGCCA 1 cut(s) 2373
Mph1103I ATGCAT 1 cut(s) 506
MroNI GCCGGC 1 cut(s) 3513
MroXI GAANNNNTTC 1 cut(s) 2011
MscI TGGCCA 1 cut(s) 2373
MseI TTAA 9 cut(s) 384, 593, 944, 986, 1250, 1397, 2270, 2529, 3306
MslI CAYNNNNRTG 5 cut(s) 416, 1515, 1724, 2103, 3324
Msp20I TGGCCA 1 cut(s) 2373
MspA1I CMGCKG 3 cut(s) 1586, 3719, 3794
MspI CCGG 2 cut(s) 3514, 3900
MvnI CGCG 1 cut(s) 2829
NaeI GCCGGC 1 cut(s) 3515
NcoI CCATGG 2 cut(s) 2098, 2640
NdeI CATATG 2 cut(s) 24, 2335
NgoMIV GCCGGC 1 cut(s) 3513
NmuCI GTSAC 2 cut(s) 221, 250
NsiI ATGCAT 1 cut(s) 506
NspI RCATGY 3 cut(s) 380, 417, 2842
NspV TTCGAA 2 cut(s) 3584, 3665
OliI CACNNNNGTG 3 cut(s) 416, 1724, 3324
PagI TCATGA 1 cut(s) 795
PasI CCCWGGG 1 cut(s) 2873
PceI AGGCCT 1 cut(s) 2681
PciI ACATGT 2 cut(s) 413, 2838
PcsI WCGNNNNNNNCGW 1 cut(s) 3775
PdiI GCCGGC 1 cut(s) 3515
PdmI GAANNNNTTC 1 cut(s) 2011
PflFI GACNNNGTC 1 cut(s) 2715
PflMI CCANNNNNTGG 2 cut(s) 1553, 3400
PfoI TCCNGGA 3 cut(s) 468, 1951, 3729
PleI GAGTC 9 cut(s) 18, 639, 960, 1568, 1741, 1974, 2285, 2335, 2420
PmaCI CACGTG 1 cut(s) 830
PmlI CACGTG 1 cut(s) 830
PpsI GAGTC 9 cut(s) 18, 639, 960, 1568, 1741, 1974, 2285, 2335, 2420
Ppu21I YACGTR 2 cut(s) 303, 830
PpuMI RGGWCCY 1 cut(s) 2936
PscI ACATGT 2 cut(s) 413, 2838
PshBI ATTAAT 1 cut(s) 3306
Psp124BI GAGCTC 2 cut(s) 1070, 3525
Psp5II RGGWCCY 1 cut(s) 2936
PspCI CACGTG 1 cut(s) 830
PspFI CCCAGC 2 cut(s) 1319, 2478
PspPI GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 2936, 3775, 3893
PspPPI RGGWCCY 1 cut(s) 2936
PsrI GAACNNNNNNTAC 2 cut(s) 3091, 3123
PstI CTGCAG 5 cut(s) 187, 2062, 2417, 3091, 3115
PstNI CAGNNNCTG 3 cut(s) 1076, 2386, 3110
PsuI RGATCY 8 cut(s) 262, 1194, 2137, 2258, 2351, 3020, 3244, 3443
PsyI GACNNNGTC 1 cut(s) 2715
PvuII CAGCTG 2 cut(s) 3719, 3794
RseI CAYNNNNRTG 5 cut(s) 416, 1515, 1724, 2103, 3324
SacI GAGCTC 2 cut(s) 1070, 3525
SaqAI TTAA 9 cut(s) 384, 593, 944, 986, 1250, 1397, 2270, 2529, 3306
Sau96I GGNCC 8 cut(s) 92, 349, 884, 1219, 1949, 2936, 3775, 3893
ScaI AGTACT 2 cut(s) 1795, 2064
SchI GAGTC 9 cut(s) 19, 640, 961, 1568, 1742, 1975, 2285, 2336, 2420
SduI GDGCHC 9 cut(s) 1070, 1189, 1414, 1790, 1928, 2344, 2857, 3525, 3919
SexAI ACCWGGT 1 cut(s) 1425
SfuI TTCGAA 2 cut(s) 3584, 3665
SinI GGWCC 5 cut(s) 92, 349, 884, 1949, 2936
SmiMI CAYNNNNRTG 5 cut(s) 416, 1515, 1724, 2103, 3324
SmlI CTYRAG 1 cut(s) 2278
SmoI CTYRAG 1 cut(s) 2278
SseBI AGGCCT 1 cut(s) 2681
SsiI CCGC 6 cut(s) 140, 1586, 2184, 2455, 2827, 3856
SspMI CTAG 7 cut(s) 911, 992, 1758, 2043, 2127, 3033, 3297
SstI GAGCTC 2 cut(s) 1070, 3525
StuI AGGCCT 1 cut(s) 2681
StyI CCWWGG 3 cut(s) 2098, 2306, 2640
TaiI ACGT 4 cut(s) 305, 832, 2050, 3546
TaqI TCGA 8 cut(s) 1414, 2067, 2710, 3310, 3553, 3584, 3665, 3769
TatI WGTACW 7 cut(s) 319, 1793, 2062, 2398, 2500, 2749, 3336
TauI GCSGC 2 cut(s) 143, 3859
Tru1I TTAA 9 cut(s) 384, 593, 944, 986, 1250, 1397, 2270, 2529, 3306
Tru9I TTAA 9 cut(s) 384, 593, 944, 986, 1250, 1397, 2270, 2529, 3306
TseFI GTSAC 2 cut(s) 221, 250
Tsp45I GTSAC 2 cut(s) 221, 250
TspDTI ATGAA 9 cut(s) 662, 1551, 1923, 2001, 2508, 2589, 2910, 2967, 3300
TspGWI ACGGA 5 cut(s) 253, 2284, 2709, 2941, 3922
Tth111I GACNNNGTC 1 cut(s) 2715
Van91I CCANNNNNTGG 2 cut(s) 1553, 3400
VpaK11BI GGWCC 5 cut(s) 92, 349, 884, 1949, 2936
VspI ATTAAT 1 cut(s) 3306
XapI RAATTY 8 cut(s) 488, 1211, 1594, 2404, 2587, 2782, 3001, 3276
XbaI TCTAGA 1 cut(s) 3032
XceI RCATGY 3 cut(s) 380, 417, 2842
XcmI CCANNNNNNNNNTGG 1 cut(s) 1726
XmnI GAANNNNTTC 1 cut(s) 2011
XspI CTAG 7 cut(s) 911, 992, 1758, 2043, 2127, 3033, 3297
ZraI GACGTC 1 cut(s) 2048
ZrmI AGTACT 2 cut(s) 1795, 2064
Zsp2I ATGCAT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.