Rroxscaffold_6G00411470

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002662
Physical Location & Seq
Reverse (-)
34000875 .. 34009185
8311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00411470.1

Sequence Viewer

Length: 4032 bp
ATGGAATCTGCTAGCCCTAACACTAACAATATGCCAGGCGTTGTCCATCAGTTGCTTCCTGTTGTTGGACCCATGCTTCTGATTGCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGTTGAAGCAGGTTCCCGTTATGGAACTGATCTGGCTGCAGTAATGCTTATATTCAATTTGGCTGCTATTTTATGTCACTATCTGTCAGCACGGATTGCTGTAGTCACTGGAAGAGATCTTGCTCAGATCCTCGGTATCGCACATGCACTTAATCTTCTGTTTGGGTGGGACTTGTTCACTTGTGTGTTTTTGACTGCTGCTAATGCTGTTTTATACCCTCTTTTTTCCACCCTCCTGGAGACTTGCAAGGCGAAATTCCTTTGCGTATGCATATACATTGCAGGATTTGTACTGCTTTCCTTTGTTCTTGGAGTATTTATCAGTCAACCACAAGTGCCACTTTCCATGACTGGGATGTTAACAAAACTGAGTGGGGAAAGTGCTTTTTCACTGATGAGTCTTCTTGGAGCAAGTATAATGCCCCACAGTTTTTATCTTCATTCTTCTATTGTGCAGCAGCATCAGCAACAACCAACTGTTTCTAAGGATGCCTTGTGTCAGAACCATTTTGTTGCCATCTTCTGCACCTTTAGTGGTATTTATCTGGTGAATTATGCCCTCATGACCTTAGCAGCAAATGTATTCTACACTTCACGTGGTTTGCTTACATTTCAGGATGCAATGTCCCTAATGGAACAGGTTTTAATTTCTCTCCATGATCACTTGCTTTTGATGAATTATGTTTACAATAATCGTTCAATACCATGCTGCACTCTCAAATTTAAGTCTTTTCAAATTATGCAGGTCTTTTGGGGTCCAATAGTACCTGTTGCCTTCTTGCTAGTTCTCTTTTTATCAAATCAAATCACAACATTAAGCTGGAGTCTGGGGGGACAAGTAGTCTTGAATGATTTTTTAAAACTAGACCTTCCTGGTTGGCTTCACTGCGCTACAATCAGAATCATTGCCATTGTTCCTGCTCTGTATTTTGTTTGGAGCTCAGGAGCTGAGGGGATGTACCAACTGCTTATATCTACACAGGTATTGGCAGCCTTGCTGCTGCCGTCTTCTGTGATCCCTCTTTTTCGTGTTGCTGCTTCAAGACAAATAATGGGAGCCCATAAGATCTCTCAGTTCATTGAATTCTTAGCCCTCATTACACTTATTGGGATGCTTGGATTAAAACTTGTCTTTGTAGTGGAAATGATTTTTGGGAATAGTGATTGGGTGGATAATTTGAGATGGGATGCTGGGAGTAGTATGTCTGCACTTCTCATCACTGCTTCTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACACCATTAAAATCTGCAAGTGCTCGATTAGAGAACCAGGTGTGGAACTGGGATATGCCAAAGGGTGTATCTGAGCCATTTAGAAATAAGGAGGAGATTGATATAGCTGAACCTAATTATCATAGAGATGCAAGTGTTCAGAAGCATGAACCATCACCATCTTCTGGGAAGGCTTTGGATAGAGACTCGGATACAGCGGTTGCAAATTTTGATTTTGTTCTGCCCGAAACTCTCTTGGAGCCTGATCGGGAGCTTCAATCAACTACCTCAGAGGAAAATAGTTCTCTTAATACCTTTCCTCGCTCCGCCAAATGCAGTAAGGAGGAATCCCCATCTGTGGTGGAGTCAATTCGCCTCCCAACTGTGGCTAGTGAGGTTTCTGATGTTACATCACTGGGCACCAGTACTGTGAAAGTTGAATCAACAGAGCCAGTTGAGAAGATTCTTGGAGTTGAAGGAGACTTACCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAACCTGAAGATTCCTTGAAAGAGGTTTCTGGGGGCACCACTTCATTGACATCGGAGGGTCCAGGATCATTCAGGAGTCTCAGTGGAAAAGCAGGGTTGGGCCGTGCTGCTAGACGTCAACTAGCTGCAGTACTCGATGAGTTTTGGGGACAACTGTATGATTTCCATGGTAATGTAATTCAAGAAGCAAAGGCTAGGAAACTGGACCTGTTGTTGGGGTCAGATTCAAAGGCTTCTTCTGCTGCTTCCTCCGTGCTGAAAGATGATACCACTGCAAAGGAAGTTTCTGGATACTTTCCATCTGTAGGAGGCAGAGGATCTGATCCTTTAATCAACTCAAGTTTATATGACTCCGTAAATCAGCCGAGGCTGCAAAACAGTTTAGAGTCAACATATGGTGCTCAAAGGGGATCTTCCTCGATATGGTCTGGCCACATACAGTTGCTGGATGCGTATGTACAAAATTCAACCCGCAGTATTATTGACTCGGGTGAGAGGCACTATTCAAGTGTGCGCGGTATACCATCTTCAGACTTGGCTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCATCTTCTGACTCGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCAACTTCTGACTCGGGTGAGAGGCGCTATTCAAGTGTCCGCAGCATACCATCTGCTGAGAGCTGGGATTACCAGCCAGCCACAGTACACGGTTATCAGATTTCATCGTATCTTAATCGTAATGACAGAAGTTCTGGTAACTTGAATGGTCAAATGGAATCACCAGCCCTAAATTCTGCTTCTTCATTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGCTCTGGACAGGCCTCCAGTTTTCAGAACCTTACGGTATCTCGACATAGTCTGTTGCAATCTGAAAGACCATATTATGATGTACACTCTTCTGGACTTTCTGAGAATGTGGTAAATTCAGCCAATGCAAAGAAATACCACAGTTTACCTGACATTCACCGCGATCTCTATATGTCTAATAAAAGTGCTCAACTGGATGCTCCCCCTGGGTTTGGGAAAACTAATTATGAATCATCCTTGTATCCAAAATCTGGTGCACGGACAGGAGGTCCTTTGGCATTTGATGAACTCTCTCCATCAAAAGTCTATAGAGATGTTCTATCATCCCAACAGAATTCTAATTTTGGCACTGGATCTCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCATTCTATTGGGACTGCAGTTGGTAGTAGAGCAGGTTCTGCAGGTCAGGAAGCTATGTCAGTTGCAGATTCAGAGGCGAAGCTTCTTCGGTCTTTTAGACACTGCATTGTGAAGCTTTTGAAGTTGGAAGGGTCTGACTGGTTGTTTAGACAGAATGATGGAGTGGATGAGGATCTAATAGATCGTGTGGCTGCCAGGGAGAAATTTCTTTATGAAGCTGAAACTAGAGAGATTAATCGAACAGTTCACATGGGTGAATCTCAGTACACTTCTGACAGGAAGTCAACTTCTGCAATAAAAATGAGTGATGCAAATCTCACCCATCTCATGGTTTCCTCAGTTCCTAATTGTGGGGAAGGTTGTATCTGGAGGTCTGATCTAATAATAAGCTTTGGGGTCTGGTGTATCCATCGGATTCTTGATTTGTCCCTTATGGAAAGCCGGCCAGAGCTCTGGGGGAAATATACTTACGTTCTCAATCGACTTCAGGGGATTATTGATTCTGCGTTTTCAAAGCCTCGTTCTCCAATGTCTCCATGCTTCTGCCTTCAAATTGCAGCAGCACAGCAGCAGAAGTCTAGTCCAACATTTTCAAATGGAATGTTACCCCCTGCTGCAAAACCAGCCAGGGGGAAATGTACTACAGCTGTAACGCTCCTGGACATAATCAAGGATGTGGAGATTGCAATCTCTTGTCGAAAGGGCCGAACGGGGACGGCAGCCGGTGATGTGGCTTTCCCGAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGGCGATTATCCAACAAACCTGTTGGCACTCAGGAGGGGCCTTCTGGTTCACGGAAGGGTGCTCCAACATCTGCTCCTTATGGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1343

Amino Acids

146.44

Weight (kDa)

6.39

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 84 4.1e-11 Natural resistance-associated macrophage protein-like
Nramp PF01566 80 - 256 9.9e-32 Natural resistance-associated macrophage protein-like
Nramp PF01566 288 - 398 2.4e-22 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2002
Acc16I TGCGCA 2 cut(s) 2447, 2495
Acc36I ACCTGC 4 cut(s) 122, 856, 3182, 3191
AccB1I GGYRCC 2 cut(s) 1781, 1919
AccB7I CCANNNNNTGG 3 cut(s) 1547, 3005, 3487
AccI GTMKAC 3 cut(s) 2404, 2452, 2500
AccII CGCG 2 cut(s) 2400, 2916
AciI CCGC 8 cut(s) 116, 1580, 1689, 2356, 2400, 2542, 2914, 3943
AclWI GGATC 8 cut(s) 244, 1132, 1957, 2201, 2209, 2302, 3115, 3339
AcoI YGGCCR 2 cut(s) 2314, 3602
AcsI RAATTY 9 cut(s) 377, 842, 1205, 1588, 2347, 2674, 2869, 3088, 3362
AcuI CTGAAG 3 cut(s) 1911, 2397, 3629
AcvI CACGTG 1 cut(s) 719
AcyI GRCGYC 1 cut(s) 1999
AhdI GACNNNNNGTC 3 cut(s) 962, 3021, 3439
AjuI GAANNNNNNNTTGG 2 cut(s) 1257, 1289
AleI CACNNNNGTG 2 cut(s) 305, 3411
Alw21I GWGCWC 7 cut(s) 1064, 1408, 2287, 2944, 3013, 3612, 4006
Alw26I GTCTC 5 cut(s) 356, 1560, 1836, 1967, 3696
Alw44I GTGCAC 1 cut(s) 3009
AlwI GGATC 8 cut(s) 244, 1132, 1957, 2201, 2209, 2302, 3115, 3339
AlwNI CAGNNNCTG 3 cut(s) 1070, 2329, 3197
Ama87I CYCGRG 3 cut(s) 2371, 2467, 2515
AoxI GGCC 6 cut(s) 1984, 2314, 2766, 3602, 3862, 3980
ApaLI GTGCAC 1 cut(s) 3009
ApoI RAATTY 9 cut(s) 377, 842, 1205, 1588, 2347, 2674, 2869, 3088, 3362
AseI ATTAAT 1 cut(s) 3393
AspLEI GCGC 7 cut(s) 1013, 2400, 2433, 2448, 2481, 2496, 2529
AspS9I GGNCC 8 cut(s) 68, 878, 1943, 1984, 2089, 3023, 3862, 3980
AsuNHI GCTAGC 1 cut(s) 11
AvaI CYCGRG 3 cut(s) 2371, 2467, 2515
AvaII GGWCC 5 cut(s) 68, 878, 1943, 2089, 3023
BaeGI GKGCMC 3 cut(s) 1784, 1922, 3013
BaeI ACNNNNGTAYC 2 cut(s) 1566, 1599
BalI TGGCCA 1 cut(s) 2316
BanI GGYRCC 2 cut(s) 1781, 1919
BanII GRGCYC 3 cut(s) 1064, 1183, 3612
BarI GAAGNNNNNNTAC 8 cut(s) 1830, 1862, 2395, 2427, 2443, 2475, 2491, 2523
BbrPI CACGTG 1 cut(s) 719
BbsI GAAGAC 2 cut(s) 515, 1122
Bbv12I GWGCWC 7 cut(s) 1064, 1408, 2287, 2944, 3013, 3612, 4006
BbvCI CCTCAGC 1 cut(s) 1071
BceAI ACGGC 3 cut(s) 1111, 1971, 3891
BciVI GTATCC 4 cut(s) 1567, 2168, 3006, 3575
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 5 cut(s) 356, 1560, 1836, 1967, 3696
BfmI CTRYAG 9 cut(s) 159, 222, 2010, 2187, 2704, 3061, 3174, 3198, 3801
BfoI RGCGCY 3 cut(s) 2434, 2482, 2530
BfuAI ACCTGC 4 cut(s) 122, 856, 3182, 3191
BfuI GTATCC 4 cut(s) 1567, 2168, 3006, 3575
BglII AGATCT 2 cut(s) 238, 1188
BmcAI AGTACT 2 cut(s) 1789, 2016
Bme18I GGWCC 5 cut(s) 68, 878, 1943, 2089, 3023
BmeRI GACNNNNNGTC 3 cut(s) 962, 3021, 3439
BmeT110I CYCGRG 3 cut(s) 2371, 2467, 2515
BmgT120I GGNCC 8 cut(s) 68, 878, 1943, 1984, 2089, 3023, 3862, 3980
BmrI ACTGGG 3 cut(s) 483, 1441, 1787
BmtI GCTAGC 1 cut(s) 15
BmuI ACTGGG 3 cut(s) 483, 1441, 1787
BpiI GAAGAC 2 cut(s) 515, 1122
BpmI CTGGAG 4 cut(s) 380, 964, 2755, 3547
Bpu10I CCTNAGC 3 cut(s) 691, 1063, 1071
BpuEI CTTGAG 1 cut(s) 2206
BsaAI YACGTR 1 cut(s) 719
BsaBI GATNNNNATC 4 cut(s) 3156, 3330, 3471, 3845
BsaHI GRCGYC 1 cut(s) 1999
BsaXI ACNNNNNCTCC 4 cut(s) 1951, 1981, 4000, 4030
Bse118I RCCGGY 2 cut(s) 3600, 3881
Bse3DI GCAATG 3 cut(s) 399, 750, 1026
Bse8I GATNNNNATC 4 cut(s) 3156, 3330, 3471, 3845
BseJI GATNNNNATC 4 cut(s) 3156, 3330, 3471, 3845
BseMI GCAATG 3 cut(s) 399, 750, 1026
BseRI GAGGAG 1 cut(s) 1490
BseSI GKGCMC 3 cut(s) 1784, 1922, 3013
BseYI CCCAGC 2 cut(s) 1313, 2565
BsgI GTGCAG 4 cut(s) 596, 631, 817, 1314
Bsh1236I CGCG 2 cut(s) 2400, 2916
BshFI GGCC 6 cut(s) 1986, 2316, 2768, 3604, 3864, 3982
BshNI GGYRCC 2 cut(s) 1781, 1919
BsiHKAI GWGCWC 7 cut(s) 1064, 1408, 2287, 2944, 3013, 3612, 4006
BsiHKCI CYCGRG 3 cut(s) 2371, 2467, 2515
BsiSI CCGG 2 cut(s) 3601, 3882
BslFI GGGAC 7 cut(s) 305, 733, 969, 2046, 3184, 3571, 3886
BsmAI GTCTC 5 cut(s) 356, 1560, 1836, 1967, 3696
BsmFI GGGAC 7 cut(s) 305, 733, 969, 2046, 3184, 3571, 3886
BsnI GGCC 6 cut(s) 1986, 2316, 2768, 3604, 3864, 3982
BsoBI CYCGRG 3 cut(s) 2371, 2467, 2515
Bsp1407I TGTACA 2 cut(s) 2341, 2836
Bsp19I CCATGG 2 cut(s) 2050, 2727
BspACI CCGC 8 cut(s) 116, 1580, 1689, 2356, 2400, 2542, 2914, 3943
BspANI GGCC 6 cut(s) 1986, 2316, 2768, 3604, 3864, 3982
BspFNI CGCG 2 cut(s) 2400, 2916
BspHI TCATGA 1 cut(s) 684
BspMAI CTGCAG 4 cut(s) 163, 2014, 3178, 3202
BspMI ACCTGC 4 cut(s) 122, 856, 3182, 3191
BspOI GCTAGC 1 cut(s) 15
BspPI GGATC 8 cut(s) 244, 1132, 1957, 2201, 2209, 2302, 3115, 3339
BspT107I GGYRCC 2 cut(s) 1781, 1919
BsrDI GCAATG 3 cut(s) 399, 750, 1026
BsrFI RCCGGY 2 cut(s) 3600, 3881
BsrGI TGTACA 2 cut(s) 2341, 2836
BssAI RCCGGY 2 cut(s) 3600, 3881
BssNAI GTATAC 3 cut(s) 2405, 2453, 2501
BssNI GRCGYC 1 cut(s) 1999
BssT1I CCWWGG 2 cut(s) 2050, 2727
Bst1107I GTATAC 3 cut(s) 2405, 2453, 2501
Bst6I CTCTTC 2 cut(s) 229, 2848
BstACI GRCGYC 1 cut(s) 1999
BstAPI GCANNNNNTGC 3 cut(s) 218, 2740, 3197
BstAUI TGTACA 2 cut(s) 2341, 2836
BstBAI YACGTR 1 cut(s) 719
BstC8I GCNNGC 4 cut(s) 13, 1377, 2580, 3602
BstDSI CCRYGG 2 cut(s) 2050, 2727
BstFNI CGCG 2 cut(s) 2400, 2916
BstH2I RGCGCY 3 cut(s) 2434, 2482, 2530
BstHHI GCGC 7 cut(s) 1013, 2400, 2433, 2448, 2481, 2496, 2529
BstMAI GTCTC 5 cut(s) 356, 1560, 1836, 1967, 3696
BstMWI GCNNNNNNNGC 9 cut(s) 218, 326, 586, 2123, 2254, 2740, 3133, 3197, 3782
BstNSI RCATGY 1 cut(s) 269
BstSFI CTRYAG 9 cut(s) 159, 222, 2010, 2187, 2704, 3061, 3174, 3198, 3801
BstSLI GKGCMC 3 cut(s) 1784, 1922, 3013
BstUI CGCG 2 cut(s) 2400, 2916
BstV2I GAAGAC 2 cut(s) 515, 1122
BstX2I RGATCY 7 cut(s) 238, 249, 1188, 2201, 2294, 3107, 3331
BstXI CCANNNNNNTGG 2 cut(s) 358, 3612
BstYI RGATCY 7 cut(s) 238, 249, 1188, 2201, 2294, 3107, 3331
BstZ17I GTATAC 3 cut(s) 2405, 2453, 2501
BsuI GTATCC 4 cut(s) 1567, 2168, 3006, 3575
BsuRI GGCC 6 cut(s) 1986, 2316, 2768, 3604, 3864, 3982
BtgI CCRYGG 2 cut(s) 2050, 2727
BtsI GCAGTG 4 cut(s) 1006, 1341, 2154, 3259
BtsIMutI CAGTG 9 cut(s) 228, 512, 1006, 1341, 1775, 1972, 2154, 3102, 3259
BveI ACCTGC 4 cut(s) 122, 856, 3182, 3191
Cac8I GCNNGC 4 cut(s) 13, 1377, 2580, 3602
CaiI CAGNNNCTG 3 cut(s) 1070, 2329, 3197
CciI TCATGA 1 cut(s) 684
CfoI GCGC 7 cut(s) 1013, 2400, 2433, 2448, 2481, 2496, 2529
Cfr10I RCCGGY 2 cut(s) 3600, 3881
Cfr13I GGNCC 8 cut(s) 68, 878, 1943, 1984, 2089, 3023, 3862, 3980
CsiI ACCWGGT 1 cut(s) 1419
DraI TTTAAA 1 cut(s) 981
DriI GACNNNNNGTC 3 cut(s) 962, 3021, 3439
EaeI YGGCCR 2 cut(s) 2314, 3602
Eam1104I CTCTTC 2 cut(s) 229, 2848
Eam1105I GACNNNNNGTC 3 cut(s) 962, 3021, 3439
EarI CTCTTC 2 cut(s) 229, 2848
EciI GGCGGA 1 cut(s) 1678
Ecl136II GAGCTC 2 cut(s) 1062, 3610
Eco130I CCWWGG 2 cut(s) 2050, 2727
Eco147I AGGCCT 1 cut(s) 2768
Eco24I GRGCYC 3 cut(s) 1064, 1183, 3612
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 5 cut(s) 68, 878, 1943, 2089, 3023
Eco53kI GAGCTC 2 cut(s) 1062, 3610
Eco57I CTGAAG 3 cut(s) 1911, 2397, 3629
Eco72I CACGTG 1 cut(s) 719
Eco88I CYCGRG 3 cut(s) 2371, 2467, 2515
EcoICRI GAGCTC 2 cut(s) 1062, 3610
EcoO109I RGGNCCY 2 cut(s) 3023, 3980
EcoRI GAATTC 2 cut(s) 1205, 3088
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 2 cut(s) 2050, 2727
EcoT22I ATGCAT 1 cut(s) 395
EcoT38I GRGCYC 3 cut(s) 1064, 1183, 3612
ErhI CCWWGG 2 cut(s) 2050, 2727
FalI AAGNNNNNCTT 6 cut(s) 447, 479, 599, 631, 2281, 2313
FaqI GGGAC 7 cut(s) 305, 733, 969, 2046, 3184, 3571, 3886
FauI CCCGC 1 cut(s) 2363
FauNDI CATATG 1 cut(s) 2278
FbaI TGATCA 1 cut(s) 781
FblI GTMKAC 3 cut(s) 2404, 2452, 2500
FriOI GRGCYC 3 cut(s) 1064, 1183, 3612
FspI TGCGCA 2 cut(s) 2447, 2495
GlaI GCGC 7 cut(s) 1012, 2399, 2432, 2447, 2480, 2495, 2528
GsaI CCCAGC 2 cut(s) 1317, 2569
GsuI CTGGAG 4 cut(s) 380, 964, 2755, 3547
HaeII RGCGCY 3 cut(s) 2434, 2482, 2530
HaeIII GGCC 6 cut(s) 1986, 2316, 2768, 3604, 3864, 3982
HapII CCGG 2 cut(s) 3601, 3882
HhaI GCGC 7 cut(s) 1013, 2400, 2433, 2448, 2481, 2496, 2529
Hin1I GRCGYC 1 cut(s) 1999
Hin6I GCGC 7 cut(s) 1011, 2398, 2431, 2446, 2479, 2494, 2527
HinP1I GCGC 7 cut(s) 1011, 2398, 2431, 2446, 2479, 2494, 2527
HincII GTYRAC 5 cut(s) 449, 483, 2003, 2274, 3444
HindII GTYRAC 5 cut(s) 449, 483, 2003, 2274, 3444
HindIII AAGCTT 3 cut(s) 3239, 3272, 3547
HpaI GTTAAC 1 cut(s) 483
HpaII CCGG 2 cut(s) 3601, 3882
HpyCH4IV ACGT 3 cut(s) 718, 1999, 3630
HpyF10VI GCNNNNNNNGC 9 cut(s) 218, 326, 586, 2123, 2254, 2740, 3133, 3197, 3782
HpySE526I ACGT 3 cut(s) 718, 1999, 3630
Hsp92I GRCGYC 1 cut(s) 1999
HspAI GCGC 7 cut(s) 1011, 2398, 2431, 2446, 2479, 2494, 2527
KroI GCCGGC 1 cut(s) 3600
KroNI GCCGGC 1 cut(s) 3602
Ksp22I TGATCA 1 cut(s) 781
KspAI GTTAAC 1 cut(s) 483
MabI ACCWGGT 1 cut(s) 1419
MaeII ACGT 3 cut(s) 718, 1999, 3630
MaeIII GTNAC 6 cut(s) 197, 226, 1768, 2639, 3762, 3808
MflI RGATCY 7 cut(s) 238, 249, 1188, 2201, 2294, 3107, 3331
MlsI TGGCCA 1 cut(s) 2316
MluNI TGGCCA 1 cut(s) 2316
MmeI TCCRAC 6 cut(s) 46, 70, 3264, 3767, 3978, 4031
Mox20I TGGCCA 1 cut(s) 2316
Mph1103I ATGCAT 1 cut(s) 395
MroNI GCCGGC 1 cut(s) 3600
MscI TGGCCA 1 cut(s) 2316
MslI CAYNNNNRTG 4 cut(s) 305, 1509, 2055, 3411
Msp20I TGGCCA 1 cut(s) 2316
MspA1I CMGCKG 2 cut(s) 1580, 3806
MspI CCGG 2 cut(s) 3601, 3882
MvnI CGCG 2 cut(s) 2400, 2916
MwoI GCNNNNNNNGC 9 cut(s) 218, 326, 586, 2123, 2254, 2740, 3133, 3197, 3782
NaeI GCCGGC 1 cut(s) 3602
NcoI CCATGG 2 cut(s) 2050, 2727
NdeI CATATG 1 cut(s) 2278
NgoMIV GCCGGC 1 cut(s) 3600
NheI GCTAGC 1 cut(s) 11
NmeAIII GCCGAG 1 cut(s) 2274
NmuCI GTSAC 2 cut(s) 197, 226
NsbI TGCGCA 2 cut(s) 2447, 2495
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 1 cut(s) 269
OliI CACNNNNGTG 2 cut(s) 305, 3411
PagI TCATGA 1 cut(s) 684
PasI CCCWGGG 1 cut(s) 2960
PceI AGGCCT 1 cut(s) 2768
PcsI WCGNNNNNNNCGW 1 cut(s) 3862
PdiI GCCGGC 1 cut(s) 3602
PflFI GACNNNGTC 1 cut(s) 2802
PflMI CCANNNNNTGG 3 cut(s) 1547, 3005, 3487
PfoI TCCNGGA 3 cut(s) 357, 1945, 3816
PmaCI CACGTG 1 cut(s) 719
PmlI CACGTG 1 cut(s) 719
Ppu21I YACGTR 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 3023
PshBI ATTAAT 1 cut(s) 3393
Psp124BI GAGCTC 2 cut(s) 1064, 3612
Psp5II RGGWCCY 1 cut(s) 3023
PspCI CACGTG 1 cut(s) 719
PspFI CCCAGC 2 cut(s) 1313, 2565
PspPI GGNCC 8 cut(s) 68, 878, 1943, 1984, 2089, 3023, 3862, 3980
PspPPI RGGWCCY 1 cut(s) 3023
PsrI GAACNNNNNNTAC 2 cut(s) 3178, 3210
PstI CTGCAG 4 cut(s) 163, 2014, 3178, 3202
PstNI CAGNNNCTG 3 cut(s) 1070, 2329, 3197
PsuI RGATCY 7 cut(s) 238, 249, 1188, 2201, 2294, 3107, 3331
PsyI GACNNNGTC 1 cut(s) 2802
PvuII CAGCTG 1 cut(s) 3806
RseI CAYNNNNRTG 4 cut(s) 305, 1509, 2055, 3411
SacI GAGCTC 2 cut(s) 1064, 3612
Sau96I GGNCC 8 cut(s) 68, 878, 1943, 1984, 2089, 3023, 3862, 3980
ScaI AGTACT 2 cut(s) 1789, 2016
SexAI ACCWGGT 1 cut(s) 1419
SfcI CTRYAG 9 cut(s) 159, 222, 2010, 2187, 2704, 3061, 3174, 3198, 3801
SinI GGWCC 5 cut(s) 68, 878, 1943, 2089, 3023
SmiMI CAYNNNNRTG 4 cut(s) 305, 1509, 2055, 3411
SmlI CTYRAG 1 cut(s) 2221
SmoI CTYRAG 1 cut(s) 2221
SseBI AGGCCT 1 cut(s) 2768
SsiI CCGC 8 cut(s) 116, 1580, 1689, 2356, 2400, 2542, 2914, 3943
SstI GAGCTC 2 cut(s) 1064, 3612
StuI AGGCCT 1 cut(s) 2768
StyI CCWWGG 2 cut(s) 2050, 2727
TaiI ACGT 3 cut(s) 721, 2002, 3633
TaqI TCGA 7 cut(s) 1408, 2019, 2303, 2797, 3397, 3640, 3856
TaqII GACCGA 1 cut(s) 3237
TatI WGTACW 8 cut(s) 412, 1787, 2014, 2341, 2587, 2836, 3423, 3797
TauI GCSGC 2 cut(s) 119, 3946
TscAI CASTG 9 cut(s) 235, 519, 1013, 1348, 1782, 1972, 2161, 3109, 3266
TseFI GTSAC 2 cut(s) 197, 226
Tsp45I GTSAC 2 cut(s) 197, 226
TspGWI ACGGA 5 cut(s) 229, 2125, 2227, 3028, 4009
TspRI CASTG 9 cut(s) 235, 519, 1013, 1348, 1782, 1972, 2161, 3109, 3266
Tth111I GACNNNGTC 1 cut(s) 2802
Van91I CCANNNNNTGG 3 cut(s) 1547, 3005, 3487
VneI GTGCAC 1 cut(s) 3009
VpaK11BI GGWCC 5 cut(s) 68, 878, 1943, 2089, 3023
VspI ATTAAT 1 cut(s) 3393
XapI RAATTY 9 cut(s) 377, 842, 1205, 1588, 2347, 2674, 2869, 3088, 3362
XbaI TCTAGA 1 cut(s) 3119
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 3 cut(s) 2404, 2452, 2500
ZraI GACGTC 1 cut(s) 2000
ZrmI AGTACT 2 cut(s) 1789, 2016
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.