MD12G1117400.v1.1

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002662
Physical Location & Seq
Reverse (-)
18745720 .. 18753215
7496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1117400.v1.1.491

Sequence Viewer

Length: 3942 bp
ATGGAATCCGCCAATCCTAATGCTAACATTATACCGGGTGTTCTACAGCGGTTGATTCCTGTTGTTGGACCTGTGCTTCTGATTTCAGTTGGATATATTGACCCTGGAAAGTGGGCAGCAACTGCTGAAGCAGGTGCCTGTTTTGGATCTGAACTTGCAGCATTGATGCTTATTTTCAATTTGGCAGCTATTTTATGTCACTATCTGTCAGCTCGGATTGGTGTAGTCACAGGAAGAGATCTTGCACAGATATGCAGTGAGGAGTATGACAAAGGCACTTGCATATCCTTAGGAATTCAAACTGAGGTTTCTGCGATCCTGTCAGACCTAACCATGGTCCTCGGCATTGCACATGGGCTTAATCTTCTGTTTGGTTGGGACTTGTTCACTTGTGTCTTTTTGACTGCTGTTAATGCCGTTCTATACCCTCTTTTTTCCACCTTCCTGGAAACTTGCAAGGTGAAGGTTGTATGCATATATGTTACAGGATTTATACTCCTTTCCTTTGTTCTTGGAGTAATTATCAGTCAACCAGAAATGCCCCATTCCATGAATGGGATGCTAACAAAGCTGAGTGGGGAGAGTGCATTTGCACTGATGAGCCTTCTTGGAGCCAGCATAATGCCTCACAGTCTGTATTTTCATTCTTCTATTGTGCAGCAATATCAGCAGCAGGCAACTGTTCCCAAGGGTGCTTTGTGTCATAACCATTTAGTTGCCATCATATGCATCTTCAGTGGTATTTATATGGTGAATTATGTCCTTATGACCTCAGCAGAAAATTTATTCTACAGTCCAGAGGCAAGTTCAGGGCTTGTTCTTACGTTTCAGGATGCAATGTCACTAATGGAACAGGTCTTTTGGGGTCCAATAGTGCCGGCTTCCTTCTTACTGGTTCTCGTTCTGTCAAATCAAATTACAACATTAAGCTGGAGTCTGGGTGGGGAAGTAGTCCTGAATGAATTTTTGAAAGTGGACCTTCCTGGTTGGCTTCATTGTGCTACAATCAGAATTATTGCCATTGTTCCAGCCCTTTATTTTGTTTGGAGTTCGGGAGCTGAAGGAATGTATCAACTGCTTATATTCACACAAGTTTTGGCAGCTCTGCTACTGCCACCTTCTGTGATCCCTATTTTTCGAATTGCTGCTTCAAGACCAAGAATGGGCGTCCATAAAATTTCTCAATTTGTTGAATTCTTATCCCTGATTACGCTTATTGGGATGCTTGGATTGAAAGTTATATTTGTAGTAGAAATGATTTTTGGGAATAGTGAATGGGGTGGTATCTTGAGGTCGAACAGTGGTAGTAGTATGTCACTATTCCTCACTGCTTGTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACCCCATTAAAATCTGCGAGTGTCCGACTAGAGGCTCAGGTGTGGAACTGGGAACTGAAGGGTACATCAGATTTGTTGAAAAATGAAGAGGAGAGTAATATAGCTGGACCTCAGTATCAGAGAGAGGCAAGTGTCCAGAACCATGAACCATCACCATCATTTGGGTTGGATGCTGATTCAGAAGTTGCAAATTTTGATCTTGATCTGCCTGACACTATCGTGGAGGCTGATCAGGAACTTAACCAAACAACTGTAGCGGAGAATAGTAATTCTCATATTACCTTTCCTAGCTCCCCTAAAAGCTGCATGGAGGGATCCACATTTGCAGTGGAGTCAGTTCCAGTCGCAACTGTCGTTAATGATGTTTCTGATGTCACATTGGAGGACACCAGTGCACTGAAGGTCAAGTCAACAGAAATTGAGAAGACAGTTGAAGTTGAAGGAGTTGAAGGAGACTTACCAACTGAAAAAGATGACGATGAGGGAGATAACTGGGAGCCTGAAGAATTATCAAAAGGGGTTTCTGGGAGCACTGCACCATTGGCCTCCGAGGGATCAGGATCCTTTAGGAGTCTAAGTGGAAAAGGTGATGATTTGGGGAGCAGTGCAGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTTCTGCAAGACGTCAACTAGCTACTATGCTTGATGAATTTTGGGGACAACTGTATGATTTCCATGGGAATGTAATTCAAGACGCAAAGGCTAAGAAGCTGGACCTTTTGTTGGGATCAGATTCAAAGGCGGTGTCCTCCTCGCTGAAAGTTGATACTGATGCAAAAGAATCTTTGGGATACTTTCCATCTCTAGGAGGCAGAGGATCTGATTCTCTTATCAACTCAAGTTTATATGACTCTGCAAAGCAGCATAGGGTTCAAAGCAGTTTAGAGTCATATGCGGTCCAAAGGGGATCTTCCCCATTGTTGCCCAGCCACATGCAGTTGTTAGATGCCTATGTACAAAGTTCAAGCCGTAGTATTATTGACTCTGATGAGAGGCACTATTCAAGTGTGTGCAGTATCCCAACTTCTGAGAGTGGTGGGAAGCGCTATCCAAGTATGCGCAGTATCCCAACTTCTGAGAGTTGGGATTACCAGCCAGCTACAGTACATGGTTATCAGATTGCATCATATCTCAATCGATTGTCAAAGGAAAAAAATTTTGATAACTTGAACGGTCAAATGGAGCCATCAATCCTAAAGTCCGGTTCTTCGTTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGATCTGGTCAGGCCTCCAATTTTCAGAACTTTACAGTATCTAGAAATAGTCCAATGCAATCTGAAAGACCGTATTATGATCCATGCTCTTCTGGAATTGTTGAGAATGTGGTAAGTCCAGCCAATGCAAAGAAATACCATAGTTTACCAGACATTCACCGGGAGCTTTACATGCCTGATAAAAATGCTAACTGGGAAAGTCCTCTCGGGTATGGATCATCTGCTGGGATGGCTGGTTATGAATCATCCTTTTATTCAAATTCTGGGGCACGAACAAGAGCTCCTTTGGCTTTTGATGAACTCTCTCCAAATGTCTACAATTCTCTGTCATCACAACAGAGTTCTACTTTTAACGCTGGTTCACTCTGGTCTAGACAGCCTTTTGAGCAGTTTGGTGTGGCTGATACTAGTCGTACTATTGGTAGTGGAATTGGTAATAGGGTAGGTTCAGTAAATCAAGAAACTACTTCAGTTGCATATTCAGAGGCCAAGCTTCTTCAGTCTTTCAGGCATTGCATTGTGAAGCTTTTGAAATTGGAAGGGTCGGACTGGTTGTTTACACAGAATGATGGGGTTGATGAGGATCTAATTGATCGTGTGGCTGCAAAGGAGAAATTTCGTTATGAAGCTGAAACTAGAGTGATAAATCAAACAGGTCAAGTGGGCGAGCTTCAGTACTGTTCTTCAGATACGGAGTCTGCTTCAGCATTGAAGAATAATGATTCGAGTTATATTATGATTTCCTCAGTTCCACATTGTGGGGATGGTTGTATATGGAAGGAAGATTTGGTAGTAAGCTTTGGGGTTTGGTGTATCCATCGCATTCTTGATTTGTCACTTATGGAAAGCCGGCCAGAGCTATGGGGGAAATATACCTACGTCCTCAACCGACTTCAGGGGATTATTGATTCGGCATTTTCAAAGCCTCGTTCTCCAATGTCACCATGCATCTGCCTTGAAGTTCCCACGGCACACCAGCTGCAATCGAGTCTGTCATTTTCTAATGGAATACCTCCTCCTGCGAAACCAGCCAGGGGAAAATGCACAACGGCAGTAACGCTTCTAGACATAATCAAGGATGTGGAGATTGCAATCAATTCTCGAAAGGGCCGAACAGGGACAGCAGCTGGTGATGTAGCTTTCCCGAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGTAGATTATCCAACAAAACTGTTGGCACTCATGAGGGTCCTGGTTCGGGCAAGTCTCTGACATCTGCTCCTTATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1314

Amino Acids

142.28

Weight (kDa)

5.25

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 394 8.7e-80 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
AatII GACGTC 1 cut(s) 2023
Acc16I TGCGCA 1 cut(s) 2456
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 134
AccB7I CCANNNNNTGG 2 cut(s) 1529, 3431
AccI GTMKAC 1 cut(s) 2988
AciI CCGC 5 cut(s) 9, 49, 1625, 2138, 2291
AcoI YGGCCR 1 cut(s) 3524
AcyI GRCGYC 2 cut(s) 1167, 2020
AdeI CACNNNGTG 1 cut(s) 3431
AfaI GTAC 5 cut(s) 1432, 2352, 2502, 3088, 3350
AfeI AGCGCT 1 cut(s) 2441
AhlI ACTAGT 1 cut(s) 3080
AjnI CCWGG 5 cut(s) 103, 444, 982, 3704, 3901
AjuI GAANNNNNNNTTGG 4 cut(s) 1245, 1277, 3443, 3475
AleI CACNNNNGTG 1 cut(s) 1586
AloI GAACNNNNNNTCC 2 cut(s) 2938, 2970
Alw21I GWGCWC 3 cut(s) 1765, 1901, 2956
Alw26I GTCTC 2 cut(s) 1815, 3921
Alw44I GTGCAC 1 cut(s) 1761
AlwNI CAGNNNCTG 2 cut(s) 122, 3800
Ama87I CYCGRG 1 cut(s) 2879
Aor51HI AGCGCT 1 cut(s) 2441
AoxI GGCC 5 cut(s) 1911, 2685, 3159, 3524, 3781
ApaLI GTGCAC 1 cut(s) 1761
ArsI GACNNNNNNTTYG 2 cut(s) 2126, 2158
Asp700I GAANNNNTTC 1 cut(s) 1984
AspLEI GCGC 2 cut(s) 2442, 2457
AspS9I GGNCC 9 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3781, 3899
AsuC2I CCSGG 2 cut(s) 36, 2834
AsuHPI GGTGA 7 cut(s) 472, 763, 1512, 1967, 2822, 3606, 3815
AsuII TTCGAA 1 cut(s) 1138
AvaI CYCGRG 1 cut(s) 2879
AvaII GGWCC 8 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3899
AxyI CCTNAGG 1 cut(s) 289
BaeGI GKGCMC 2 cut(s) 1765, 2944
BaeI ACNNNNGTAYC 2 cut(s) 1466, 1499
BamHI GGATCC 2 cut(s) 1682, 1928
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 2956
BarI GAAGNNNNNNTAC 6 cut(s) 1809, 1841, 2404, 2436, 2452, 2484
BbsI GAAGAC 1 cut(s) 1799
Bbv12I GWGCWC 3 cut(s) 1765, 1901, 2956
BbvCI CCTCAGC 1 cut(s) 772
BccI CCATC 9 cut(s) 728, 1525, 1531, 2203, 2590, 2896, 3236, 3431, 3498
BceAI ACGGC 4 cut(s) 401, 2349, 3657, 3738
BciT130I CCWGG 5 cut(s) 105, 446, 984, 3706, 3903
BciVI GTATCC 4 cut(s) 2180, 2423, 2471, 3497
BclI TGATCA 1 cut(s) 1597
BcnI CCSGG 2 cut(s) 36, 2834
BcoDI GTCTC 2 cut(s) 1815, 3921
BcuI ACTAGT 1 cut(s) 3080
BfaI CTAG 9 cut(s) 1397, 1656, 2027, 2201, 2715, 3045, 3081, 3309, 3737
BfmI CTRYAG 5 cut(s) 44, 790, 1620, 2496, 2623
BfoI RGCGCY 1 cut(s) 2443
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 4 cut(s) 2180, 2423, 2471, 3497
BglII AGATCT 1 cut(s) 238
BmcAI AGTACT 1 cut(s) 3350
Bme1390I CCNGG 7 cut(s) 36, 105, 446, 984, 2834, 3706, 3903
Bme18I GGWCC 8 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3899
BmeT110I CYCGRG 1 cut(s) 2879
BmgT120I GGNCC 9 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3781, 3899
BmiI GGNNCC 8 cut(s) 136, 613, 867, 1684, 1866, 1930, 2580, 3900
BmrFI CCNGG 7 cut(s) 36, 105, 446, 984, 2834, 3706, 3903
BmrI ACTGGG 3 cut(s) 1426, 1870, 2875
BmuI ACTGGG 3 cut(s) 1426, 1870, 2875
BpiI GAAGAC 1 cut(s) 1799
BpmI CTGGAG 1 cut(s) 952
Bpu10I CCTNAGC 2 cut(s) 772, 1404
Bpu14I TTCGAA 1 cut(s) 1138
BpuEI CTTGAG 2 cut(s) 1309, 2218
BpuMI CCSGG 2 cut(s) 36, 2834
Bsa29I ATCGAT 1 cut(s) 2533
BsaBI GATNNNNATC 4 cut(s) 1569, 1927, 3255, 3764
BsaHI GRCGYC 2 cut(s) 1167, 2020
BsaJI CCNNGG 9 cut(s) 103, 333, 340, 687, 1917, 2071, 2646, 3639, 3705
BsaWI WCCGGW 1 cut(s) 2597
BsaXI ACNNNNNCTCC 7 cut(s) 925, 955, 1930, 1960, 2938, 2968, 3913
Bse118I RCCGGY 2 cut(s) 877, 3522
Bse1I ACTGG 7 cut(s) 897, 1421, 1709, 1758, 1865, 2870, 3227
Bse21I CCTNAGG 1 cut(s) 289
Bse3DI GCAATG 3 cut(s) 345, 843, 3184
Bse8I GATNNNNATC 4 cut(s) 1569, 1927, 3255, 3764
BseBI CCWGG 5 cut(s) 105, 446, 984, 3706, 3903
BseCI ATCGAT 1 cut(s) 2533
BseDI CCNNGG 9 cut(s) 103, 333, 340, 687, 1917, 2071, 2646, 3639, 3705
BseGI GGATG 8 cut(s) 564, 838, 1227, 1543, 2907, 2918, 3442, 3757
BseJI GATNNNNATC 4 cut(s) 1569, 1927, 3255, 3764
BseMI GCAATG 3 cut(s) 345, 843, 3184
BseMII CTCAG 8 cut(s) 294, 563, 786, 1418, 1493, 2415, 2463, 3432
BseNI ACTGG 7 cut(s) 897, 1421, 1709, 1758, 1865, 2870, 3227
BseRI GAGGAG 4 cut(s) 275, 1472, 2137, 3678
BseSI GKGCMC 2 cut(s) 1765, 2944
BseYI CCCAGC 2 cut(s) 2321, 2897
BsgI GTGCAG 4 cut(s) 677, 1887, 1995, 2428
BshFI GGCC 5 cut(s) 1913, 2687, 3161, 3526, 3783
BshNI GGYRCC 1 cut(s) 134
BshVI ATCGAT 1 cut(s) 2533
BsiHKAI GWGCWC 3 cut(s) 1765, 1901, 2956
BsiHKCI CYCGRG 1 cut(s) 2879
BsiSI CCGG 5 cut(s) 35, 878, 2598, 2833, 3523
BslFI GGGAC 3 cut(s) 392, 2067, 3805
BsmAI GTCTC 2 cut(s) 1815, 3921
BsmFI GGGAC 3 cut(s) 392, 2067, 3805
BsmI GAATGC 1 cut(s) 3495
BsnI GGCC 5 cut(s) 1913, 2687, 3161, 3526, 3783
BsoBI CYCGRG 1 cut(s) 2879
Bsp119I TTCGAA 1 cut(s) 1138
Bsp1286I GDGCHC 4 cut(s) 1765, 1901, 2944, 2956
Bsp1407I TGTACA 1 cut(s) 2350
Bsp19I CCATGG 3 cut(s) 333, 2071, 2646
BspACI CCGC 5 cut(s) 9, 49, 1625, 2138, 2291
BspANI GGCC 5 cut(s) 1913, 2687, 3161, 3526, 3783
BspCNI CTCAG 8 cut(s) 295, 564, 785, 1417, 1492, 2416, 2464, 3431
BspDI ATCGAT 1 cut(s) 2533
BspHI TCATGA 1 cut(s) 3892
BspLI GGNNCC 8 cut(s) 136, 613, 867, 1684, 1866, 1930, 2580, 3900
BspMI ACCTGC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 2767
BspT104I TTCGAA 1 cut(s) 1138
BspT107I GGYRCC 1 cut(s) 134
BsrDI GCAATG 3 cut(s) 345, 843, 3184
BsrFI RCCGGY 2 cut(s) 877, 3522
BsrGI TGTACA 1 cut(s) 2350
BsrI ACTGG 7 cut(s) 897, 1421, 1709, 1758, 1865, 2870, 3227
BssAI RCCGGY 2 cut(s) 877, 3522
BssECI CCNNGG 9 cut(s) 103, 333, 340, 687, 1917, 2071, 2646, 3639, 3705
BssNI GRCGYC 2 cut(s) 1167, 2020
BssT1I CCWWGG 4 cut(s) 333, 687, 2071, 2646
Bst2UI CCWGG 5 cut(s) 105, 446, 984, 3706, 3903
Bst6I CTCTTC 3 cut(s) 229, 1449, 2767
BstACI GRCGYC 2 cut(s) 1167, 2020
BstAPI GCANNNNNTGC 2 cut(s) 122, 2659
BstAUI TGTACA 1 cut(s) 2350
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 7 cut(s) 616, 675, 879, 1362, 2493, 3341, 3524
BstDSI CCRYGG 4 cut(s) 333, 2071, 2646, 3639
BstF5I GGATG 8 cut(s) 564, 838, 1227, 1543, 2907, 2918, 3442, 3757
BstH2I RGCGCY 1 cut(s) 2443
BstHHI GCGC 2 cut(s) 2442, 2457
BstMAI GTCTC 2 cut(s) 1815, 3921
BstNI CCWGG 5 cut(s) 105, 446, 984, 3706, 3903
BstNSI RCATGY 2 cut(s) 2332, 2848
BstSCI CCNGG 7 cut(s) 34, 103, 444, 982, 2832, 3704, 3901
BstSFI CTRYAG 5 cut(s) 44, 790, 1620, 2496, 2623
BstSLI GKGCMC 2 cut(s) 1765, 2944
BstV2I GAAGAC 1 cut(s) 1799
BstX2I RGATCY 8 cut(s) 146, 238, 1682, 1928, 2213, 2303, 2675, 3256
BstXI CCANNNNNNTGG 2 cut(s) 445, 3534
BstYI RGATCY 8 cut(s) 146, 238, 1682, 1928, 2213, 2303, 2675, 3256
Bsu15I ATCGAT 1 cut(s) 2533
Bsu36I CCTNAGG 1 cut(s) 289
BsuI GTATCC 4 cut(s) 2180, 2423, 2471, 3497
BsuRI GGCC 5 cut(s) 1913, 2687, 3161, 3526, 3783
BsuTUI ATCGAT 1 cut(s) 2533
BtgI CCRYGG 4 cut(s) 333, 2071, 2646, 3639
BtgZI GCGATG 1 cut(s) 3476
BtsCI GGATG 8 cut(s) 564, 838, 1227, 1543, 2907, 2918, 3442, 3757
BtsI GCAGTG 5 cut(s) 262, 1326, 1701, 1899, 1978
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 7 cut(s) 616, 675, 879, 1362, 2493, 3341, 3524
CaiI CAGNNNCTG 2 cut(s) 122, 3800
CciI TCATGA 1 cut(s) 3892
CfoI GCGC 2 cut(s) 2442, 2457
Cfr10I RCCGGY 2 cut(s) 877, 3522
Cfr13I GGNCC 9 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3781, 3899
ClaI ATCGAT 1 cut(s) 2533
CseI GACGC 2 cut(s) 1156, 2099
Csp6I GTAC 5 cut(s) 1431, 2351, 2501, 3087, 3349
CviQI GTAC 5 cut(s) 1431, 2351, 2501, 3087, 3349
DraIII CACNNNGTG 1 cut(s) 3431
EaeI YGGCCR 1 cut(s) 3524
Eam1104I CTCTTC 3 cut(s) 229, 1449, 2767
EarI CTCTTC 3 cut(s) 229, 1449, 2767
Ecl136II GAGCTC 1 cut(s) 2954
Eco130I CCWWGG 4 cut(s) 333, 687, 2071, 2646
Eco147I AGGCCT 1 cut(s) 2687
Eco24I GRGCYC 1 cut(s) 2956
Eco47I GGWCC 8 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3899
Eco47III AGCGCT 1 cut(s) 2441
Eco53kI GAGCTC 1 cut(s) 2954
Eco81I CCTNAGG 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 2879
EcoICRI GAGCTC 1 cut(s) 2954
EcoO109I RGGNCCY 1 cut(s) 3899
EcoRI GAATTC 2 cut(s) 294, 1193
EcoRII CCWGG 5 cut(s) 103, 444, 982, 3704, 3901
EcoT14I CCWWGG 4 cut(s) 333, 687, 2071, 2646
EcoT22I ATGCAT 3 cut(s) 476, 731, 3623
EcoT38I GRGCYC 1 cut(s) 2956
ErhI CCWWGG 4 cut(s) 333, 687, 2071, 2646
FalI AAGNNNNNCTT 6 cut(s) 963, 995, 2290, 2322, 2941, 2973
FaqI GGGAC 3 cut(s) 392, 2067, 3805
FauNDI CATATG 2 cut(s) 725, 2287
FbaI TGATCA 1 cut(s) 1597
FblI GTMKAC 1 cut(s) 2988
FokI GGATG 8 cut(s) 571, 845, 1234, 1550, 2905, 2914, 3449, 3764
FriOI GRGCYC 1 cut(s) 2956
FspBI CTAG 9 cut(s) 1397, 1656, 2027, 2201, 2715, 3045, 3081, 3309, 3737
FspI TGCGCA 1 cut(s) 2456
GlaI GCGC 2 cut(s) 2441, 2456
GsaI CCCAGC 2 cut(s) 2325, 2901
GsuI CTGGAG 1 cut(s) 952
HaeII RGCGCY 1 cut(s) 2443
HaeIII GGCC 5 cut(s) 1913, 2687, 3161, 3526, 3783
HapII CCGG 5 cut(s) 35, 878, 2598, 2833, 3523
HgaI GACGC 2 cut(s) 1156, 2099
HhaI GCGC 2 cut(s) 2442, 2457
Hin1I GRCGYC 2 cut(s) 1167, 2020
Hin6I GCGC 2 cut(s) 2440, 2455
HinP1I GCGC 2 cut(s) 2440, 2455
HincII GTYRAC 3 cut(s) 530, 1779, 2024
HindII GTYRAC 3 cut(s) 530, 1779, 2024
HindIII AAGCTT 3 cut(s) 3164, 3197, 3469
HpaII CCGG 5 cut(s) 35, 878, 2598, 2833, 3523
HphI GGTGA 7 cut(s) 472, 763, 1512, 1967, 2822, 3606, 3815
HpyCH4IV ACGT 3 cut(s) 824, 2020, 3552
HpySE526I ACGT 3 cut(s) 824, 2020, 3552
Hsp92I GRCGYC 2 cut(s) 1167, 2020
HspAI GCGC 2 cut(s) 2440, 2455
KroI GCCGGC 2 cut(s) 877, 3522
KroNI GCCGGC 2 cut(s) 879, 3524
Ksp22I TGATCA 1 cut(s) 1597
LguI GCTCTTC 1 cut(s) 2767
MaeI CTAG 9 cut(s) 1397, 1656, 2027, 2201, 2715, 3045, 3081, 3309, 3737
MaeII ACGT 3 cut(s) 824, 2020, 3552
MaeIII GTNAC 9 cut(s) 197, 226, 481, 840, 1314, 1741, 3507, 3612, 3727
MflI RGATCY 8 cut(s) 146, 238, 1682, 1928, 2213, 2303, 2675, 3256
MhlI GDGCHC 4 cut(s) 1765, 1901, 2944, 2956
MlyI GAGTC 8 cut(s) 943, 1709, 1948, 2240, 2291, 2372, 3377, 3670
MmeI TCCRAC 6 cut(s) 46, 70, 1417, 1515, 3198, 3897
Mph1103I ATGCAT 3 cut(s) 476, 731, 3623
MroNI GCCGGC 2 cut(s) 877, 3522
MroXI GAANNNNTTC 1 cut(s) 1984
MseI TTAA 7 cut(s) 360, 411, 926, 1376, 1608, 1725, 3024
MslI CAYNNNNRTG 3 cut(s) 250, 1586, 2076
MspA1I CMGCKG 3 cut(s) 49, 3652, 3800
MspI CCGG 5 cut(s) 35, 878, 2598, 2833, 3523
MspR9I CCNGG 7 cut(s) 36, 105, 446, 984, 2834, 3706, 3903
Mva1269I GAATGC 1 cut(s) 3495
MvaI CCWGG 5 cut(s) 105, 446, 984, 3706, 3903
NaeI GCCGGC 2 cut(s) 879, 3524
NciI CCSGG 2 cut(s) 36, 2834
NcoI CCATGG 3 cut(s) 333, 2071, 2646
NdeI CATATG 2 cut(s) 725, 2287
NgoMIV GCCGGC 2 cut(s) 877, 3522
NlaIV GGNNCC 8 cut(s) 136, 613, 867, 1684, 1866, 1930, 2580, 3900
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 7 cut(s) 197, 226, 840, 1314, 1741, 3507, 3612
NsbI TGCGCA 1 cut(s) 2456
NsiI ATGCAT 3 cut(s) 476, 731, 3623
NspI RCATGY 2 cut(s) 2332, 2848
NspV TTCGAA 1 cut(s) 1138
OliI CACNNNNGTG 1 cut(s) 1586
PagI TCATGA 1 cut(s) 3892
PaqCI CACCTGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 2687
PciSI GCTCTTC 1 cut(s) 2767
PcsI WCGNNNNNNNCGW 3 cut(s) 1719, 3781, 3859
PctI GAATGC 1 cut(s) 3495
PdiI GCCGGC 2 cut(s) 879, 3524
PdmI GAANNNNTTC 1 cut(s) 1984
PflMI CCANNNNNTGG 2 cut(s) 1529, 3431
PfoI TCCNGGA 1 cut(s) 444
PleI GAGTC 8 cut(s) 942, 1708, 1947, 2240, 2290, 2372, 3376, 3669
PpsI GAGTC 8 cut(s) 942, 1708, 1947, 2240, 2290, 2372, 3376, 3669
PpuMI RGGWCCY 1 cut(s) 3899
Psp124BI GAGCTC 1 cut(s) 2956
Psp5II RGGWCCY 1 cut(s) 3899
Psp6I CCWGG 5 cut(s) 103, 444, 982, 3704, 3901
PspFI CCCAGC 2 cut(s) 2321, 2897
PspGI CCWGG 5 cut(s) 103, 444, 982, 3704, 3901
PspN4I GGNNCC 8 cut(s) 136, 613, 867, 1684, 1866, 1930, 2580, 3900
PspPI GGNCC 9 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3781, 3899
PspPPI RGGWCCY 1 cut(s) 3899
PstNI CAGNNNCTG 2 cut(s) 122, 3800
PsuI RGATCY 8 cut(s) 146, 238, 1682, 1928, 2213, 2303, 2675, 3256
PvuII CAGCTG 2 cut(s) 3652, 3800
RsaI GTAC 5 cut(s) 1432, 2352, 2502, 3088, 3350
RsaNI GTAC 5 cut(s) 1431, 2351, 2501, 3087, 3349
RseI CAYNNNNRTG 3 cut(s) 250, 1586, 2076
SacI GAGCTC 1 cut(s) 2956
SapI GCTCTTC 1 cut(s) 2767
SaqAI TTAA 7 cut(s) 360, 411, 926, 1376, 1608, 1725, 3024
Sau96I GGNCC 9 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3781, 3899
ScaI AGTACT 1 cut(s) 3350
SchI GAGTC 8 cut(s) 943, 1709, 1948, 2240, 2291, 2372, 3377, 3670
ScrFI CCNGG 7 cut(s) 36, 105, 446, 984, 2834, 3706, 3903
SduI GDGCHC 4 cut(s) 1765, 1901, 2944, 2956
SfcI CTRYAG 5 cut(s) 44, 790, 1620, 2496, 2623
SfuI TTCGAA 1 cut(s) 1138
SinI GGWCC 8 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3899
SmiMI CAYNNNNRTG 3 cut(s) 250, 1586, 2076
SmlI CTYRAG 2 cut(s) 1288, 2233
SmoI CTYRAG 2 cut(s) 1288, 2233
SpeI ACTAGT 1 cut(s) 3080
SseBI AGGCCT 1 cut(s) 2687
SsiI CCGC 5 cut(s) 9, 49, 1625, 2138, 2291
SspMI CTAG 9 cut(s) 1397, 1656, 2027, 2201, 2715, 3045, 3081, 3309, 3737
SstI GAGCTC 1 cut(s) 2956
StuI AGGCCT 1 cut(s) 2687
StyD4I CCNGG 7 cut(s) 34, 103, 444, 982, 2832, 3704, 3901
StyI CCWWGG 4 cut(s) 333, 687, 2071, 2646
TaiI ACGT 3 cut(s) 827, 2023, 3555
TaqI TCGA 6 cut(s) 1138, 1295, 2533, 3398, 3659, 3775
TatI WGTACW 3 cut(s) 2350, 2500, 3348
Tru1I TTAA 7 cut(s) 360, 411, 926, 1376, 1608, 1725, 3024
Tru9I TTAA 7 cut(s) 360, 411, 926, 1376, 1608, 1725, 3024
TseFI GTSAC 7 cut(s) 197, 226, 840, 1314, 1741, 3507, 3612
Tsp45I GTSAC 7 cut(s) 197, 226, 840, 1314, 1741, 3507, 3612
TspGWI ACGGA 1 cut(s) 3380
Van91I CCANNNNNTGG 2 cut(s) 1529, 3431
VneI GTGCAC 1 cut(s) 1761
VpaK11BI GGWCC 8 cut(s) 68, 337, 866, 976, 1475, 2110, 2293, 3899
XbaI TCTAGA 3 cut(s) 2714, 3044, 3736
XceI RCATGY 2 cut(s) 2332, 2848
XcmI CCANNNNNNNNNTGG 2 cut(s) 551, 1693
XmiI GTMKAC 1 cut(s) 2988
XmnI GAANNNNTTC 1 cut(s) 1984
XspI CTAG 9 cut(s) 1397, 1656, 2027, 2201, 2715, 3045, 3081, 3309, 3737
ZraI GACGTC 1 cut(s) 2021
ZrmI AGTACT 1 cut(s) 3350
Zsp2I ATGCAT 3 cut(s) 476, 731, 3623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.