Prupe.6G235600_v2.0.a1

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0002662
Physical Location & Seq
Reverse (-)
23652869 .. 23662260
9392 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G235600.3

Sequence Viewer

Length: 3915 bp
ATGGCTAATTTGGAATCTGCTAATCCTAGTGCTAACAATATGTTGGGCGTTCTACATCGGTTGCTTCCTGTTGTTGGACCGGCGCTTCTGATTTCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGCTGAAGCAGGTGCCCGTTTTGGCTCTGACCTGGCAGCATTGATGCTTATTTTCAATTTTGCAGCTATTTTATGTCACTATCTGTCAGCTCGGATTGGTGTAGTCACTGGAAGAGATCTTGCGCAGATATGCAGTGAGGAGTATGACAAAGGCACATGCATATTCTTAGGAGTTCAAACAGAGGTTTCTGTGATTCTGTCAGACCTTACCATGATCCTCGGTATCGCACATGGACTTAATCTTCTGTTTGGGTGGGACTTGTTCACTTGCGTGTTTTTGACTGCTGTTAATGCTGTTTTGTACCCTCTTTTTTCCACCCTCCTGGAGACTTGCAAGGCGAAGGTCCTATGCGTATGCATTGCGGGATTTATACAGCTTTCCTTCGTTCTTGGAGTAATTATCAGTCAACCAGAAATGTCATTTTCCATGAATGGGATGCTAACAAAGCTGAGTGGGGAGAGTGCCTTTGCATTGATGAGTCTTCTTGGAGCAAGTATAATGCCTCACAGTCTCTATCTTCATTCTTCGATTGTGCAGCAGTATCAGTGTCAGCCAACTGTTTCCAGGGATGCTTTGTGTCACCACCATTTAGTTGCCATCTTGTGCATCTTTAGTGGTATTTATCTGGTGAATTATGCCCTTATGACCTCAGCGGAGAATGAATACTCGGGCCTTGGTTTGCTTACATTTCAGGATGTAATGTCACTAATCGGACAGGTCTTTTGGGGTCCAATAGTATCTGGTGCCTTCCTGCTGGTTCTCTTTGTTTCAAATCAAATCACAACATTAAGCTGGAGTCTGGGTGGACAAGTAGTTTTGAATGATTTTTTGAAACTAGACCTTCCTGGTTGGCTTCATTGTGCCACAATCAGAATTATTGCTATTGTTCCAGCTCTTTATTTCGTTTGGAGTTCAGGAGCTGAAGGGATGTATCAACTTCTTATATTCACACAAGTGTTGGCAGCTCTGCTACTGCCATCTTCTGTGATCCCTCTTTTTCGAATTGCTGCTTCAAGACCAATAATGGGTGTCCATAAAGTTTCTCAATTTGTTGAATTTTTATCCCTGATTACACTAATTGGGATGCTTGGATTGAAAATCATATTTGTAGTAGAAGTGATTGTTGGGAATAGTGATTGGGTTAATAATTTGAGGTCGAATGCCGGGAGTAGTATGTCTGTTCCTTGTGTACTTCTACTCACTGCTTGTGCAACATTTTGTTTGATGATTTGGCTGGCAGCTACCCCATTAAAATCTGCAAGTGCACGATTAGAGGCTCAGGTGTGGAACTGGGATATGCATATGGGTTCACCTGATTCAATAACAAAGAAAGAGGAGATCAATATTTCTGAACCTAAATATCATAGAGAGGTAAGTGTCCAGAAGCATGAACCATCACCATCATTTGGGAGGGCTTTGGATAGTGATTCAGAAGTAGCGAGTTTTGATCTTGATCTGCCTGAAACTATCACAGAGCCTGATGAGGAGCATCACCTAACTACTGTAGTGGAAAATGGTTCTCGTATTACCTTTCCTCATTCCCCTAAATGCCATATGGAGGGATCCACATCTACAGTGGAGTCAACTCCAGTCTCAACTGTGGTTAATGAGGTTTCTGATGTTACATTGGAGGGCACCAGTGCATTGAAGATCGAATCAACAGAGCCAATTGAAAAGACTGTTGGAGTTGAAGGAGTTGAAGGAGACTTACCAAATGAAAAAGATGATGATGAGGGAGATACCTGGGAGCCTGAAGATTCATTGAAAGGGGTTTCTGAGAGCACTGCTCCATTGACATCTGAGGGTCCAGGATCATTTAGGAGTCTAAGTGGAAAAGGTGACGAAGGGGGGAGTAGTGCTGGTAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCGAGACGTCAACTAGCTGCAGTACTTGACGAATTTTGGGGACAGCTGTATGATTTCCATGGGAATGTAATTCAAGAAGCAAAGGCTAAGAAACTGGATCTTTTGTTGGGGTTAGATTCAAAGGCTGCGTCCTCCTCATTGAAAGTTGATACTAGTGCAAAGGAGCTTTCCGGATATTTTCCATCTGCAGGAGGCAGAGGATCTGATCCTATTATGAACTCAAGTTTATATGACTCTCCGAAGCAGCAGAGGGTTCAAAGCAGTTTAGAGTCATATGGGGTCCAAAGGGGATCTTCCGCATTGTTGCCCAGCCGTGTGCAGTTGTTGGATGCCTATGTGCAAAATTCAAGCCGCAGCGTTATTGACTCTGGTGAGAGGCGCTATTCAAGTGTGCGCAGTCTCCCGTCTTCTGAGAGTTGGGACTACCAGCCAGCCACAATACATAGTTATCATCCCTCATATCTCAATCGAATTGCAAAGGACAGAGGTTTTGATAATTTGAACGGTCAAATGGAGTCAGCAGCCCTACAATCCGCTTCTTCATTGGGTGCTGCAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTACAAAATGGGTTAGGCTCTGGTCAGGCCTCCATTTTCCAAAATCATACAGTATCTAGAAATAGTCCGTTGCAATCTGAAAGACCGTATTATGATCTGCACCCTTCTGGAATTGCTGAGAATGTGGTAAGTTCAGCAAATGCAAAGAAATACCATAGTTTACCCGACATTCACCGGGATCTTTACATGCCGGAGAAAAGTGCCAACTGGGAAAGTCCTGTGGGGTATGGATCATCTACTGGGATAACAAATTATGAATCATCCTTGTATTCAAATTCTGGAGCACGAACAGGAGCTCCTTTGGCATTCGATCAACTCTCTCCATCACAAGTCTACAGAGATGCTTTTTCATCACAGCAAAATTCTAGTTTTAACACTGGATCCCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCGTACTATTGGGAGTGGAGGATTTGGTTATCGGGCAGGTTCTGTAAGTCAAGAAGCTACTTCAGTTGCAGATTCAGAGGCCAAGCTTCTTCAGTCTTTCAGGCATTGCATTGTGAAACTTTTGAAATTGGAAGGATCTGACTGGTTGTTTACGCAGAATGATGGGGTTGATGAGGATCTAATTGATCGCGTGGCTGCAAGGGAGAAATTTCTTTATGAAGCTGAAACTAGAGAGATGAATCGAACAGTTCACATGGGTGAACCTCAATACCATCCTTCTGATAGGAAGTCTGTTTCTGCATTGAAGAATAATGATGCAAATTGCACCTCTTTTATGGTTCCTACTTGTGGGGAGGGTTGTATTTGGAGATCAGATTTGATAGTAAGCTTCGGGGTCTGGTGTATCCATCGTATTCTTGATTTGTCACTCATGGAAAGCCGGCCAGAGCTATGGGGGAAATATACCTACGTCCTCAACCGACTTCAGGGGATTATTGATTCAGCATTTTCAAAGCCTCGCACTCCAATGTCGCCATGCTTCTGCCTTCAAATTTCTGCAGTACACCAGCTGAAGTCAAGTCCATCTTTTTCAAATGGAATACCCCCTGCTGCAAAACCAGCCAGGGGAAAATGCACAACGGCAGTAACGCTTCTAGACATAATCAAGGATGTGGAGATTGCAATATCTTGTCGTAAGGGCCGAACGGGGACAGCAGCTGGCGATGTAGCTTTCCCGAAGGGAAAAGAAAATCTGGCGTCTGTACTCAAACGCTACAAGCGTCGATTAACCAACAAAACTGCTGGCGCTCACGAGGGTCCTGGTTCACGCAAGGTTCAGACATCTGCTCCTTATGGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1305

Amino Acids

141.48

Weight (kDa)

5.76

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 131
AatII GACGTC 1 cut(s) 2038
Acc16I TGCGCA 2 cut(s) 255, 2423
Acc36I ACCTGC 2 cut(s) 131, 3080
AccB1I GGYRCC 3 cut(s) 143, 875, 1766
AccB7I CCANNNNNTGG 1 cut(s) 1538
AccI GTMKAC 1 cut(s) 2955
AccII CGCG 1 cut(s) 3243
AccIII TCCGGA 1 cut(s) 2198
AciI CCGC 6 cut(s) 125, 494, 785, 2325, 2380, 2562
AcoI YGGCCR 1 cut(s) 3494
AcsI RAATTY 7 cut(s) 1187, 2060, 2371, 2896, 2983, 3260, 3603
AcuI CTGAAG 7 cut(s) 156, 1074, 1905, 3099, 3128, 3521, 3644
AcyI GRCGYC 2 cut(s) 2035, 3809
AfaI GTAC 6 cut(s) 434, 1323, 2052, 3058, 3615, 3816
AfiI CCNNNNNNNGG 9 cut(s) 74, 564, 1157, 1538, 1690, 2216, 3401, 3538, 3677
AhlI ACTAGT 1 cut(s) 2180
AjnI CCWGG 9 cut(s) 112, 162, 453, 695, 976, 1874, 1939, 3674, 3871
AjuI GAANNNNNNNTTGG 4 cut(s) 1239, 1271, 1797, 1829
AleI CACNNNNGTG 3 cut(s) 401, 1085, 3309
AloI GAACNNNNNNTCC 2 cut(s) 2902, 2934
Alw21I GWGCWC 4 cut(s) 1399, 1916, 2908, 2920
Alw26I GTCTC 6 cut(s) 452, 647, 1729, 1830, 2026, 2432
Alw44I GTGCAC 1 cut(s) 1395
AlwNI CAGNNNCTG 4 cut(s) 1052, 1610, 3095, 3770
Ama87I CYCGRG 1 cut(s) 799
Aor13HI TCCGGA 1 cut(s) 2198
AoxI GGCC 5 cut(s) 802, 2649, 3132, 3494, 3751
ApaLI GTGCAC 1 cut(s) 1395
ApoI RAATTY 7 cut(s) 1187, 2060, 2371, 2896, 2983, 3260, 3603
ArsI GACNNNNNNTTYG 6 cut(s) 837, 869, 2141, 2173, 2501, 2533
AspLEI GCGC 5 cut(s) 85, 256, 2409, 2424, 3860
AspS9I GGNCC 8 cut(s) 77, 475, 802, 860, 1937, 2308, 3751, 3869
AsuC2I CCSGG 2 cut(s) 1297, 2798
AsuHPI GGTGA 9 cut(s) 704, 772, 1434, 1521, 1616, 1982, 2411, 2786, 3323
AsuII TTCGAA 1 cut(s) 1132
AvaI CYCGRG 1 cut(s) 799
AvaII GGWCC 6 cut(s) 77, 475, 860, 1937, 2308, 3869
BaeGI GKGCMC 3 cut(s) 148, 1399, 1769
BamHI GGATCC 2 cut(s) 1694, 3002
BanI GGYRCC 3 cut(s) 143, 875, 1766
BanII GRGCYC 1 cut(s) 2920
BarI GAAGNNNNNNTAC 2 cut(s) 1824, 1856
BauI CACGAG 1 cut(s) 3863
BbsI GAAGAC 2 cut(s) 605, 2427
Bbv12I GWGCWC 4 cut(s) 1399, 1916, 2908, 2920
BbvCI CCTCAGC 1 cut(s) 781
BceAI ACGGC 2 cut(s) 2325, 3708
BciT130I CCWGG 9 cut(s) 114, 164, 455, 697, 978, 1876, 1941, 3676, 3873
BciVI GTATCC 1 cut(s) 3467
BcnI CCSGG 2 cut(s) 1297, 2798
BcoDI GTCTC 6 cut(s) 452, 647, 1729, 1830, 2026, 2432
BcuI ACTAGT 1 cut(s) 2180
BfaI CTAG 9 cut(s) 27, 968, 2042, 2181, 2679, 2988, 3015, 3282, 3707
BfmI CTRYAG 7 cut(s) 1635, 1704, 2046, 2214, 2587, 2956, 3609
BfoI RGCGCY 3 cut(s) 86, 2410, 3861
BfuAI ACCTGC 2 cut(s) 131, 3080
BfuI GTATCC 1 cut(s) 3467
BglII AGATCT 1 cut(s) 247
BmcAI AGTACT 1 cut(s) 2052
Bme18I GGWCC 6 cut(s) 77, 475, 860, 1937, 2308, 3869
BmeT110I CYCGRG 1 cut(s) 799
BmgT120I GGNCC 8 cut(s) 77, 475, 802, 860, 1937, 2308, 3751, 3869
BmrI ACTGGG 3 cut(s) 1432, 2839, 2871
BmsI GCATC 9 cut(s) 165, 558, 691, 747, 1206, 1630, 2347, 2953, 3358
BmuI ACTGGG 3 cut(s) 1432, 2839, 2871
BpiI GAAGAC 2 cut(s) 605, 2427
BplI GAGNNNNNCTC 2 cut(s) 1903, 1935
BpmI CTGGAG 4 cut(s) 476, 946, 1704, 2922
Bpu10I CCTNAGC 2 cut(s) 781, 1410
Bpu14I TTCGAA 1 cut(s) 1132
BpuEI CTTGAG 1 cut(s) 2233
BpuMI CCSGG 2 cut(s) 1297, 2798
BsaBI GATNNNNATC 4 cut(s) 1584, 1699, 3051, 3228
BsaHI GRCGYC 2 cut(s) 2035, 3809
BsaJI CCNNGG 8 cut(s) 112, 349, 696, 805, 1875, 2086, 2610, 3675
BsaWI WCCGGW 1 cut(s) 2198
BsaXI ACNNNNNCTCC 8 cut(s) 919, 949, 1945, 1975, 2902, 2932, 3883, 3913
Bsc4I CCNNNNNNNGG 9 cut(s) 74, 564, 1157, 1538, 1690, 2216, 3401, 3538, 3677
Bse118I RCCGGY 2 cut(s) 79, 3492
Bse1I ACTGG 9 cut(s) 244, 1427, 1721, 1770, 2127, 2834, 2866, 3004, 3200
Bse3DI GCAATG 2 cut(s) 489, 3157
Bse8I GATNNNNATC 4 cut(s) 1584, 1699, 3051, 3228
BseAI TCCGGA 1 cut(s) 2198
BseBI CCWGG 9 cut(s) 114, 164, 455, 697, 978, 1876, 1941, 3676, 3873
BseDI CCNNGG 8 cut(s) 112, 349, 696, 805, 1875, 2086, 2610, 3675
BseJI GATNNNNATC 4 cut(s) 1584, 1699, 3051, 3228
BseLI CCNNNNNNNGG 9 cut(s) 74, 564, 1157, 1538, 1690, 2216, 3401, 3538, 3677
BseMI GCAATG 2 cut(s) 489, 3157
BseMII CTCAG 7 cut(s) 572, 795, 1424, 1899, 1923, 2430, 2730
BseNI ACTGG 9 cut(s) 244, 1427, 1721, 1770, 2127, 2834, 2866, 3004, 3200
BseRI GAGGAG 4 cut(s) 284, 1481, 1631, 2152
BseSI GKGCMC 3 cut(s) 148, 1399, 1769
BseYI CCCAGC 1 cut(s) 2336
BsgI GTGCAG 3 cut(s) 686, 2366, 2705
Bsh1236I CGCG 1 cut(s) 3243
BshFI GGCC 5 cut(s) 804, 2651, 3134, 3496, 3753
BshNI GGYRCC 3 cut(s) 143, 875, 1766
BsiHKAI GWGCWC 4 cut(s) 1399, 1916, 2908, 2920
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 6 cut(s) 80, 1296, 2199, 2797, 2813, 3493
BslFI GGGAC 4 cut(s) 401, 2082, 2462, 3775
BslI CCNNNNNNNGG 9 cut(s) 74, 564, 1157, 1538, 1690, 2216, 3401, 3538, 3677
BsmAI GTCTC 6 cut(s) 452, 647, 1729, 1830, 2026, 2432
BsmBI CGTCTC 1 cut(s) 2026
BsmFI GGGAC 4 cut(s) 401, 2082, 2462, 3775
BsmI GAATGC 2 cut(s) 1297, 2927
BsnI GGCC 5 cut(s) 804, 2651, 3134, 3496, 3753
BsoBI CYCGRG 1 cut(s) 799
Bsp119I TTCGAA 1 cut(s) 1132
Bsp1286I GDGCHC 6 cut(s) 148, 1399, 1769, 1916, 2908, 2920
Bsp13I TCCGGA 1 cut(s) 2198
Bsp19I CCATGG 2 cut(s) 2086, 2610
BspACI CCGC 6 cut(s) 125, 494, 785, 2325, 2380, 2562
BspANI GGCC 5 cut(s) 804, 2651, 3134, 3496, 3753
BspCNI CTCAG 7 cut(s) 573, 794, 1423, 1900, 1924, 2431, 2731
BspEI TCCGGA 1 cut(s) 2198
BspFNI CGCG 1 cut(s) 3243
BspMAI CTGCAG 3 cut(s) 2050, 2218, 3613
BspMI ACCTGC 2 cut(s) 131, 3080
BspT104I TTCGAA 1 cut(s) 1132
BspT107I GGYRCC 3 cut(s) 143, 875, 1766
BsrDI GCAATG 2 cut(s) 489, 3157
BsrFI RCCGGY 2 cut(s) 79, 3492
BsrI ACTGG 9 cut(s) 244, 1427, 1721, 1770, 2127, 2834, 2866, 3004, 3200
BssAI RCCGGY 2 cut(s) 79, 3492
BssECI CCNNGG 8 cut(s) 112, 349, 696, 805, 1875, 2086, 2610, 3675
BssNI GRCGYC 2 cut(s) 2035, 3809
BssSI CACGAG 1 cut(s) 3863
BssT1I CCWWGG 3 cut(s) 805, 2086, 2610
Bst2BI CACGAG 1 cut(s) 3863
Bst2UI CCWGG 9 cut(s) 114, 164, 455, 697, 978, 1876, 1941, 3676, 3873
Bst6I CTCTTC 1 cut(s) 238
BstACI GRCGYC 2 cut(s) 2035, 3809
BstBI TTCGAA 1 cut(s) 1132
BstC8I GCNNGC 6 cut(s) 1368, 2025, 2460, 3494, 3772, 3856
BstDSI CCRYGG 2 cut(s) 2086, 2610
BstFNI CGCG 1 cut(s) 3243
BstH2I RGCGCY 3 cut(s) 86, 2410, 3861
BstHHI GCGC 5 cut(s) 85, 256, 2409, 2424, 3860
BstMAI GTCTC 6 cut(s) 452, 647, 1729, 1830, 2026, 2432
BstMWI GCNNNNNNNGC 6 cut(s) 131, 422, 577, 2924, 3028, 3671
BstNI CCWGG 9 cut(s) 114, 164, 455, 697, 978, 1876, 1941, 3676, 3873
BstNSI RCATGY 2 cut(s) 291, 2812
BstSFI CTRYAG 7 cut(s) 1635, 1704, 2046, 2214, 2587, 2956, 3609
BstSLI GKGCMC 3 cut(s) 148, 1399, 1769
BstUI CGCG 1 cut(s) 3243
BstV2I GAAGAC 2 cut(s) 605, 2427
BstX2I RGATCY 9 cut(s) 247, 1694, 2125, 2228, 2318, 2800, 3002, 3188, 3229
BstXI CCANNNNNNTGG 2 cut(s) 454, 3504
BstYI RGATCY 9 cut(s) 247, 1694, 2125, 2228, 2318, 2800, 3002, 3188, 3229
BsuI GTATCC 1 cut(s) 3467
BsuRI GGCC 5 cut(s) 804, 2651, 3134, 3496, 3753
BtgI CCRYGG 2 cut(s) 2086, 2610
BtgZI GCGATG 1 cut(s) 3789
BtsI GCAGTG 3 cut(s) 271, 1332, 1914
BtsIMutI CAGTG 8 cut(s) 237, 271, 683, 1332, 1713, 1777, 1914, 2997
BveI ACCTGC 2 cut(s) 131, 3080
Cac8I GCNNGC 6 cut(s) 1368, 2025, 2460, 3494, 3772, 3856
CaiI CAGNNNCTG 4 cut(s) 1052, 1610, 3095, 3770
CfoI GCGC 5 cut(s) 85, 256, 2409, 2424, 3860
Cfr10I RCCGGY 2 cut(s) 79, 3492
Cfr13I GGNCC 8 cut(s) 77, 475, 802, 860, 1937, 2308, 3751, 3869
CseI GACGC 3 cut(s) 2145, 3798, 3821
Csp6I GTAC 6 cut(s) 433, 1322, 2051, 3057, 3614, 3815
CviQI GTAC 6 cut(s) 433, 1322, 2051, 3057, 3614, 3815
EaeI YGGCCR 1 cut(s) 3494
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 2918
Eco130I CCWWGG 3 cut(s) 805, 2086, 2610
Eco147I AGGCCT 1 cut(s) 2651
Eco24I GRGCYC 1 cut(s) 2920
Eco32I GATATC 1 cut(s) 104
Eco47I GGWCC 6 cut(s) 77, 475, 860, 1937, 2308, 3869
Eco53kI GAGCTC 1 cut(s) 2918
Eco57I CTGAAG 7 cut(s) 156, 1074, 1905, 3099, 3128, 3521, 3644
Eco88I CYCGRG 1 cut(s) 799
EcoICRI GAGCTC 1 cut(s) 2918
EcoO109I RGGNCCY 2 cut(s) 475, 3869
EcoRII CCWGG 9 cut(s) 112, 162, 453, 695, 976, 1874, 1939, 3674, 3871
EcoRV GATATC 1 cut(s) 104
EcoT14I CCWWGG 3 cut(s) 805, 2086, 2610
EcoT22I ATGCAT 3 cut(s) 293, 491, 1434
EcoT38I GRGCYC 1 cut(s) 2920
ErhI CCWWGG 3 cut(s) 805, 2086, 2610
Esp3I CGTCTC 1 cut(s) 2026
FalI AAGNNNNNCTT 4 cut(s) 2305, 2337, 3622, 3654
FaqI GGGAC 4 cut(s) 401, 2082, 2462, 3775
FauI CCCGC 1 cut(s) 487
FauNDI CATATG 3 cut(s) 1434, 1686, 2302
FblI GTMKAC 1 cut(s) 2955
FriOI GRGCYC 1 cut(s) 2920
FspBI CTAG 9 cut(s) 27, 968, 2042, 2181, 2679, 2988, 3015, 3282, 3707
FspI TGCGCA 2 cut(s) 255, 2423
GlaI GCGC 5 cut(s) 84, 255, 2408, 2423, 3859
GsaI CCCAGC 1 cut(s) 2340
GsuI CTGGAG 4 cut(s) 476, 946, 1704, 2922
HaeII RGCGCY 3 cut(s) 86, 2410, 3861
HaeIII GGCC 5 cut(s) 804, 2651, 3134, 3496, 3753
HapII CCGG 6 cut(s) 80, 1296, 2199, 2797, 2813, 3493
HgaI GACGC 3 cut(s) 2145, 3798, 3821
HhaI GCGC 5 cut(s) 85, 256, 2409, 2424, 3860
Hin1I GRCGYC 2 cut(s) 2035, 3809
Hin6I GCGC 5 cut(s) 83, 254, 2407, 2422, 3858
HinP1I GCGC 5 cut(s) 83, 254, 2407, 2422, 3858
HincII GTYRAC 3 cut(s) 539, 1716, 2039
HindII GTYRAC 3 cut(s) 539, 1716, 2039
HindIII AAGCTT 2 cut(s) 3137, 3439
HpaII CCGG 6 cut(s) 80, 1296, 2199, 2797, 2813, 3493
HphI GGTGA 9 cut(s) 704, 772, 1434, 1521, 1616, 1982, 2411, 2786, 3323
Hpy99I CGWCG 1 cut(s) 3837
HpyCH4IV ACGT 2 cut(s) 2035, 3522
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 422, 577, 2924, 3028, 3671
HpySE526I ACGT 2 cut(s) 2035, 3522
Hsp92I GRCGYC 2 cut(s) 2035, 3809
HspAI GCGC 5 cut(s) 83, 254, 2407, 2422, 3858
Kpn2I TCCGGA 1 cut(s) 2198
KroI GCCGGC 1 cut(s) 3492
KroNI GCCGGC 1 cut(s) 3494
LweI GCATC 9 cut(s) 165, 558, 691, 747, 1206, 1630, 2347, 2953, 3358
MaeI CTAG 9 cut(s) 27, 968, 2042, 2181, 2679, 2988, 3015, 3282, 3707
MaeII ACGT 2 cut(s) 2035, 3522
MaeIII GTNAC 9 cut(s) 206, 235, 710, 834, 1753, 1970, 2622, 3477, 3697
MfeI CAATTG 1 cut(s) 1800
MflI RGATCY 9 cut(s) 247, 1694, 2125, 2228, 2318, 2800, 3002, 3188, 3229
MhlI GDGCHC 6 cut(s) 148, 1399, 1769, 1916, 2908, 2920
MlyI GAGTC 8 cut(s) 619, 937, 1721, 1963, 2255, 2306, 2387, 2552
MmeI TCCRAC 4 cut(s) 55, 79, 1795, 2334
Mph1103I ATGCAT 3 cut(s) 293, 491, 1434
MroI TCCGGA 1 cut(s) 2198
MroNI GCCGGC 1 cut(s) 3492
MseI TTAA 8 cut(s) 369, 420, 920, 1275, 1382, 1737, 2994, 3839
MslI CAYNNNNRTG 5 cut(s) 401, 1085, 2091, 3309, 3578
MspA1I CMGCKG 4 cut(s) 785, 2074, 3622, 3770
MspI CCGG 6 cut(s) 80, 1296, 2199, 2797, 2813, 3493
MunI CAATTG 1 cut(s) 1800
Mva1269I GAATGC 2 cut(s) 1297, 2927
MvaI CCWGG 9 cut(s) 114, 164, 455, 697, 978, 1876, 1941, 3676, 3873
MvnI CGCG 1 cut(s) 3243
MwoI GCNNNNNNNGC 6 cut(s) 131, 422, 577, 2924, 3028, 3671
NaeI GCCGGC 1 cut(s) 3494
NciI CCSGG 2 cut(s) 1297, 2798
NcoI CCATGG 2 cut(s) 2086, 2610
NdeI CATATG 3 cut(s) 1434, 1686, 2302
NgoMIV GCCGGC 1 cut(s) 3492
NmuCI GTSAC 6 cut(s) 206, 235, 710, 834, 1970, 3477
NsbI TGCGCA 2 cut(s) 255, 2423
NsiI ATGCAT 3 cut(s) 293, 491, 1434
NspI RCATGY 2 cut(s) 291, 2812
NspV TTCGAA 1 cut(s) 1132
OliI CACNNNNGTG 3 cut(s) 401, 1085, 3309
PaqCI CACCTGC 1 cut(s) 131
PceI AGGCCT 1 cut(s) 2651
PcsI WCGNNNNNNNCGW 2 cut(s) 3751, 3829
PctI GAATGC 2 cut(s) 1297, 2927
PdiI GCCGGC 1 cut(s) 3494
PflMI CCANNNNNTGG 1 cut(s) 1538
PfoI TCCNGGA 2 cut(s) 453, 1939
PleI GAGTC 8 cut(s) 618, 936, 1720, 1962, 2255, 2305, 2387, 2551
PpsI GAGTC 8 cut(s) 618, 936, 1720, 1962, 2255, 2305, 2387, 2551
PpuMI RGGWCCY 2 cut(s) 475, 3869
Psp124BI GAGCTC 1 cut(s) 2920
Psp5II RGGWCCY 2 cut(s) 475, 3869
Psp6I CCWGG 9 cut(s) 112, 162, 453, 695, 976, 1874, 1939, 3674, 3871
PspFI CCCAGC 1 cut(s) 2336
PspGI CCWGG 9 cut(s) 112, 162, 453, 695, 976, 1874, 1939, 3674, 3871
PspPI GGNCC 8 cut(s) 77, 475, 802, 860, 1937, 2308, 3751, 3869
PspPPI RGGWCCY 2 cut(s) 475, 3869
PstI CTGCAG 3 cut(s) 2050, 2218, 3613
PstNI CAGNNNCTG 4 cut(s) 1052, 1610, 3095, 3770
PsuI RGATCY 9 cut(s) 247, 1694, 2125, 2228, 2318, 2800, 3002, 3188, 3229
PvuII CAGCTG 3 cut(s) 2074, 3622, 3770
RsaI GTAC 6 cut(s) 434, 1323, 2052, 3058, 3615, 3816
RsaNI GTAC 6 cut(s) 433, 1322, 2051, 3057, 3614, 3815
RseI CAYNNNNRTG 5 cut(s) 401, 1085, 2091, 3309, 3578
SacI GAGCTC 1 cut(s) 2920
SaqAI TTAA 8 cut(s) 369, 420, 920, 1275, 1382, 1737, 2994, 3839
Sau96I GGNCC 8 cut(s) 77, 475, 802, 860, 1937, 2308, 3751, 3869
ScaI AGTACT 1 cut(s) 2052
SchI GAGTC 8 cut(s) 619, 937, 1721, 1963, 2255, 2306, 2387, 2552
SduI GDGCHC 6 cut(s) 148, 1399, 1769, 1916, 2908, 2920
SfaNI GCATC 9 cut(s) 165, 558, 691, 747, 1206, 1630, 2347, 2953, 3358
SfcI CTRYAG 7 cut(s) 1635, 1704, 2046, 2214, 2587, 2956, 3609
SfuI TTCGAA 1 cut(s) 1132
SinI GGWCC 6 cut(s) 77, 475, 860, 1937, 2308, 3869
SmiMI CAYNNNNRTG 5 cut(s) 401, 1085, 2091, 3309, 3578
SmlI CTYRAG 1 cut(s) 2248
SmoI CTYRAG 1 cut(s) 2248
SpeI ACTAGT 1 cut(s) 2180
SseBI AGGCCT 1 cut(s) 2651
SsiI CCGC 6 cut(s) 125, 494, 785, 2325, 2380, 2562
SspI AATATT 1 cut(s) 1477
SspMI CTAG 9 cut(s) 27, 968, 2042, 2181, 2679, 2988, 3015, 3282, 3707
SstI GAGCTC 1 cut(s) 2920
StuI AGGCCT 1 cut(s) 2651
StyI CCWWGG 3 cut(s) 805, 2086, 2610
TaiI ACGT 2 cut(s) 2038, 3525
TaqI TCGA 8 cut(s) 659, 1132, 1289, 1785, 2497, 2931, 3295, 3835
TatI WGTACW 4 cut(s) 1321, 2050, 3613, 3814
TauI GCSGC 2 cut(s) 128, 2382
Tru1I TTAA 8 cut(s) 369, 420, 920, 1275, 1382, 1737, 2994, 3839
Tru9I TTAA 8 cut(s) 369, 420, 920, 1275, 1382, 1737, 2994, 3839
TscAI CASTG 8 cut(s) 244, 271, 683, 1339, 1713, 1777, 1921, 3004
TseFI GTSAC 6 cut(s) 206, 235, 710, 834, 1970, 3477
Tsp45I GTSAC 6 cut(s) 206, 235, 710, 834, 1970, 3477
TspGWI ACGGA 1 cut(s) 2679
TspRI CASTG 8 cut(s) 244, 271, 683, 1339, 1713, 1777, 1921, 3004
Van91I CCANNNNNTGG 1 cut(s) 1538
VneI GTGCAC 1 cut(s) 1395
VpaK11BI GGWCC 6 cut(s) 77, 475, 860, 1937, 2308, 3869
XapI RAATTY 7 cut(s) 1187, 2060, 2371, 2896, 2983, 3260, 3603
XbaI TCTAGA 3 cut(s) 2678, 3014, 3706
XceI RCATGY 2 cut(s) 291, 2812
XcmI CCANNNNNNNNNTGG 1 cut(s) 1705
XmiI GTMKAC 1 cut(s) 2955
XspI CTAG 9 cut(s) 27, 968, 2042, 2181, 2679, 2988, 3015, 3282, 3707
ZraI GACGTC 1 cut(s) 2036
ZrmI AGTACT 1 cut(s) 2052
Zsp2I ATGCAT 3 cut(s) 293, 491, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.