Rorug03G0104600

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002662
Physical Location & Seq
Forward (+)
8296036 .. 8298039
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0104600.1

Sequence Viewer

Length: 642 bp
ATGTCCTATGCAGTGCCGGTGCCTGCGGCTGGGCCGAGCTCTCAGCCGCTGGCAAACCCCGTGACGGTGGTTAGCCCGCAGTTCCAAGCAACCTACCCTGTGGATCTGGTCATCACTGAGAAGATGATGTCGATCAAGGAAGGTGCTTTTACAGTGTCAGACGTTAATGGAAATGTCATGTTCCAGATTAAAGGCTCCTTGTTCAGCTTTCATGATCGTCGAACTTTGGTCGACAGCGCCGGCACTCCTATAGTCTCCTTCCGACAAAAGATATTGACGGCACATAGGAGATGGCACGTATTTAGAGGAGAGAGCTCAGACGCCAAAGATCTACTCTTTAGTGTCAAAAAGTCATCCCTTTTCCAACTGAAGGCAGAATTAGATGTGTTCTTGGCTGTTAATACTAAAGAACAAGCGTACGATTTCAAGGTCAAAGGAAGCTGGGGGGAAAGATCATGCACCATATATGATGGAAACAACAATATCATTGCACAAATGCACAAGAAACATGATCTTAAAAGTATATTCTTTGGGAGAGATGCTTTTGCGGTGACTGTGTATCCTCATGTTGATTACGCCTTTATAGTTGCCATTGTGGTTGTTCTTCATGAGATTAACATGGACAGAAGTGGGCAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

213

Amino Acids

23.79

Weight (kDa)

8.59

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOR PF04525 34 - 202 8.5e-53 LURP-one-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccI GTMKAC 1 cut(s) 231
AciI CCGC 4 cut(s) 26, 47, 77, 548
AclWI GGATC 1 cut(s) 111
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 3 cut(s) 29, 64, 370
AgsI TTSAA 1 cut(s) 427
AluBI AGCT 4 cut(s) 39, 207, 315, 441
AluI AGCT 4 cut(s) 39, 207, 315, 441
Alw21I GWGCWC 2 cut(s) 41, 317
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 49
AoxI GGCC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 562
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 2 cut(s) 41, 317
Bbv12I GWGCWC 2 cut(s) 41, 317
BccI CCATC 2 cut(s) 285, 464
BceAI ACGGC 1 cut(s) 294
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 259
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 240
BfuI GTATCC 1 cut(s) 570
BglII AGATCT 1 cut(s) 328
BisI GCNGC 2 cut(s) 27, 47
BlsI GCNGC 2 cut(s) 28, 48
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 2 cut(s) 21, 196
BmsI GCATC 1 cut(s) 529
BsaAI YACGTR 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 321
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 64, 370
Bse118I RCCGGY 2 cut(s) 16, 239
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 131
BseGI GGATG 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 131
BseLI CCNNNNNNNGG 3 cut(s) 29, 64, 370
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 3 cut(s) 56, 108, 330
BseRI GAGGAG 1 cut(s) 321
BseYI CCCAGC 2 cut(s) 29, 441
BshFI GGCC 1 cut(s) 34
BshNI GGYRCC 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 41, 317
BsiSI CCGG 2 cut(s) 17, 240
BsiWI CGTACG 1 cut(s) 417
BslI CCNNNNNNNGG 3 cut(s) 29, 64, 370
BsmAI GTCTC 1 cut(s) 259
BsnI GGCC 1 cut(s) 34
Bsp1286I GDGCHC 2 cut(s) 41, 317
Bsp143I GATC 6 cut(s) 103, 132, 214, 328, 452, 511
BspACI CCGC 4 cut(s) 26, 47, 77, 548
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 3 cut(s) 55, 109, 329
BspHI TCATGA 2 cut(s) 211, 607
BspLI GGNNCC 2 cut(s) 21, 196
BspPI GGATC 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 2 cut(s) 16, 239
BssAI RCCGGY 2 cut(s) 16, 239
BssMI GATC 6 cut(s) 103, 132, 214, 328, 452, 511
BssNI GRCGYC 1 cut(s) 321
Bst4CI ACNGT 3 cut(s) 67, 154, 556
BstACI GRCGYC 1 cut(s) 321
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 4 cut(s) 24, 51, 77, 241
BstDEI CTNAG 3 cut(s) 42, 117, 316
BstF5I GGATG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 6 cut(s) 106, 135, 217, 331, 455, 514
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 6 cut(s) 103, 132, 214, 328, 452, 511
BstSFI CTRYAG 1 cut(s) 249
BstX2I RGATCY 2 cut(s) 103, 328
BstYI RGATCY 2 cut(s) 103, 328
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 1 cut(s) 34
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 3 cut(s) 18, 114, 159
Cac8I GCNNGC 4 cut(s) 24, 51, 77, 241
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 2 cut(s) 211, 607
CfoI GCGC 1 cut(s) 239
Cfr10I RCCGGY 2 cut(s) 16, 239
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 418
CviAII CATG 7 cut(s) 178, 212, 456, 509, 566, 608, 619
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 3 cut(s) 42, 117, 316
DpnI GATC 6 cut(s) 105, 134, 216, 330, 454, 513
DpnII GATC 6 cut(s) 103, 132, 214, 328, 452, 511
Ecl136II GAGCTC 2 cut(s) 39, 315
Eco24I GRGCYC 2 cut(s) 41, 317
Eco53kI GAGCTC 2 cut(s) 39, 315
Eco57I CTGAAG 1 cut(s) 389
EcoICRI GAGCTC 2 cut(s) 39, 315
EcoT38I GRGCYC 2 cut(s) 41, 317
FaeI CATG 7 cut(s) 181, 215, 459, 512, 569, 611, 622
FatI CATG 7 cut(s) 177, 211, 455, 508, 565, 607, 618
FauI CCCGC 1 cut(s) 84
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 27, 47
FokI GGATG 1 cut(s) 340
FriOI GRGCYC 2 cut(s) 41, 317
Fsp4HI GCNGC 2 cut(s) 27, 47
GlaI GCGC 1 cut(s) 238
GluI GCNGC 2 cut(s) 27, 47
GsaI CCCAGC 2 cut(s) 33, 445
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 1 cut(s) 34
HapII CCGG 2 cut(s) 17, 240
HgaI GACGC 1 cut(s) 329
HhaI GCGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 7 cut(s) 181, 215, 459, 512, 569, 611, 622
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HpaII CCGG 2 cut(s) 17, 240
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 3 cut(s) 160, 263, 319
Hpy188III TCNNGA 3 cut(s) 184, 212, 608
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 222
HpyAV CCTTC 3 cut(s) 134, 268, 364
HpyCH4III ACNGT 3 cut(s) 67, 154, 556
HpyCH4IV ACGT 2 cut(s) 162, 297
HpyCH4V TGCA 4 cut(s) 11, 459, 491, 499
HpyF3I CTNAG 3 cut(s) 42, 117, 316
HpySE526I ACGT 2 cut(s) 162, 297
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 7 cut(s) 181, 215, 459, 512, 569, 611, 622
HspAI GCGC 1 cut(s) 237
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 6 cut(s) 103, 132, 214, 328, 452, 511
LmnI GCTCC 1 cut(s) 200
LweI GCATC 1 cut(s) 529
MaeII ACGT 2 cut(s) 162, 297
MaeIII GTNAC 2 cut(s) 61, 550
MalI GATC 6 cut(s) 105, 134, 216, 330, 454, 513
MboI GATC 6 cut(s) 103, 132, 214, 328, 452, 511
MboII GAAGA 2 cut(s) 133, 596
MflI RGATCY 2 cut(s) 103, 328
MhlI GDGCHC 2 cut(s) 41, 317
MluCI AATT 1 cut(s) 377
MmeI TCCRAC 2 cut(s) 286, 388
MnlI CCTC 2 cut(s) 299, 573
MroNI GCCGGC 1 cut(s) 239
MseI TTAA 5 cut(s) 165, 189, 399, 516, 615
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 2 cut(s) 17, 240
NaeI GCCGGC 1 cut(s) 241
NdeII GATC 6 cut(s) 103, 132, 214, 328, 452, 511
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 7 cut(s) 181, 215, 459, 512, 569, 611, 622
NlaIV GGNNCC 2 cut(s) 21, 196
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 61, 550
PagI TCATGA 2 cut(s) 211, 607
PdiI GCCGGC 1 cut(s) 241
Pfl23II CGTACG 1 cut(s) 417
PkrI GCNGC 2 cut(s) 28, 48
Ppu21I YACGTR 1 cut(s) 298
Psp124BI GAGCTC 2 cut(s) 41, 317
PspFI CCCAGC 2 cut(s) 29, 441
PspLI CGTACG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 21, 196
PspPI GGNCC 1 cut(s) 32
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 2 cut(s) 103, 328
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SacI GAGCTC 2 cut(s) 41, 317
SalI GTCGAC 1 cut(s) 230
SaqAI TTAA 5 cut(s) 165, 189, 399, 516, 615
SatI GCNGC 2 cut(s) 27, 47
Sau3AI GATC 6 cut(s) 103, 132, 214, 328, 452, 511
Sau96I GGNCC 1 cut(s) 32
SduI GDGCHC 2 cut(s) 41, 317
SetI ASST 9 cut(s) 41, 95, 145, 165, 209, 300, 317, 432, 443
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 249
Sse9I AATT 1 cut(s) 377
SsiI CCGC 4 cut(s) 26, 47, 77, 548
SstI GAGCTC 2 cut(s) 41, 317
TaaI ACNGT 3 cut(s) 67, 154, 556
TaiI ACGT 2 cut(s) 165, 300
TaqI TCGA 3 cut(s) 131, 220, 231
TasI AATT 1 cut(s) 377
TauI GCSGC 2 cut(s) 29, 49
Tru1I TTAA 5 cut(s) 165, 189, 399, 516, 615
Tru9I TTAA 5 cut(s) 165, 189, 399, 516, 615
TscAI CASTG 3 cut(s) 18, 121, 159
TseFI GTSAC 2 cut(s) 61, 550
Tsp45I GTSAC 2 cut(s) 61, 550
TspDTI ATGAA 2 cut(s) 200, 596
TspRI CASTG 3 cut(s) 18, 121, 159
XmiI GTMKAC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.