MD04G1096400.v1.1

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002662
Physical Location & Seq
Reverse (-)
17761120 .. 17768581
7462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1096400.v1.1.491

Sequence Viewer

Length: 3990 bp
ATGGAATCCACCAATCCTAATGCTAACATTATGCCGGGTGTTCTACAGCGGTTGCTTCCTGTTGTTGGACCTGTTCTTGTGATTTCAGTTGGATATATTGACCCTGGAAAGTGGGCAGCAACTGCTGAAGCAGGTGCCCGTTTTGGCTCTGACCTCGCAGCATTGATGCTTATTTTCAATTTTGCAGCTATTTTATGTCACTATTTGTCAGCTCGGATTGGTGTAGTCACCGGAAGAGATCTTGCACAGATATGCAGTGAGGAGTATGACAAGGGCACATGCATATCCTTAGGAATTCAAACAGAGGTTTCTGCGATTCTGTCAGACCTAACCATGATCCTCGGCATCGCACATGGGCTTAATCTTCTGTTTGGTTGGGACTTGTTCACTTGTGTCTTTTTGACTGCCGTTAATGCCATTTTGTACCCTCTTTTTTCCTCCTTCCTGGAAACTTGCAAGGCGAAGGTCCTATGCATATATGTTGCAGGATTCATACTCCTTTCCTTTGTTCTTGGAGTAATTATCAGTCAACCAGAAATGCCCCGTTCCATGAATGGGATGCTAACAAAGCTGAGTGGGGAGAGTGCATTTGCGCTGATGAGTCTTCTTGGTGCAAGCATGATGCCTCACAGTCTTTATTTTCATTCTTCTATAGTGCAGCAATGTCAGCAGCAGGCAACTGTTCCCAAGGGTGCTTTGTGTCATAACCATTTAGTTGCCATATTATGCATCTTCAGTGGTGTTTATATGGTGAATTATGTCTTTATGACCTCAGCGGAAAATTTATTCTACAGTCCAGAGGCAAGTTCTGGCCTTGTTGTGCTTACGTTTCAGGATGCAATGTCGCTAATGGAACAGGTCTTTTGGGGTCCAATAGTACCGGCTTCCTTCTTACTGGTTCTCATTCTGTCAAATCAAATCACAGCATTAAGCTGGAGTCTGGGTGGAGATGTAGTCCTGAATGAATTTTTGAAAGTGGACCTTCCTGGTTGGCTTCATTGCGCTACTATCAGAATTATTGCCATTGTTCCAGCCCTTTATTTTGTTTGGAGTTCAGGAGCCGAAGGAATGTATCAACTGCTTATATTCACACAAGTTTTGGCAGCTCTGCTACTACCATCTTCTGTGATCCCTATTTTTAGAATTGCTGCTTCAAGACCAATAATGGGTGCCCATAAAATTTCACAATTTGTTGAATTCTTATCCCTAATTACGCTTATTGGGATGCTTGGATTGAAAATTATATTTGTAGTAGAAATGATTTTTGGGAATAGTGAATGGGGTGGTAATTTAAGGTCGAATGGCGGGAGTAGTATGTTACTATTCCTCACTGCTTGTGCATCATTTGGTTTGATGATTTGGCTAGCTGCTACCCCATTAAAATCTGCAAGTGTCCGATTAGAGGCTCAGGTGTGGAACTGGGAACTGATGGAGGGTACACCAGATTCATTGACGAATGAAGAAGAGATTAACAGAGCTGAACCTCAATATCATAGAGAGGCAAGTGTCCAGAGCCATGAACCGTCACCATCATTTGGGTTGGATGCTGATTCAGAAGTTGCAAATTTTGATCTGGATCTAGCTGATACTATCATGGAGGCTGATCAGGAACTTCACCAAACAACTGTAGCAGAGAATAATAATTCTCATATTACCTTTCCTTGCTCCCCTAAAAGCCGCATGGAGGGATCCAGATCTGCAGTGGAGTCATTTCCAGTCTCAACTGTTGTTAATGATGTTTCTGATGTCACATTGGAGGACACCAGTGCACTGAAGTTCAAGTCAACAGAGTCAATTGAGAAGACTGTTGAAGCTGAAGCAGACTTACCAACCGAAAAAGATGATGATGAGGGAGATACCTGGGAGCCTGAAGAATCATCAAAAGGGGTTTCTGGGAGCACTGTGCCATTGGCCTCTGAGGGCTCGGGATCATTTAGGAGTCTAAGTGGAAAAGGCGACGATGGAGGAAGTAGTGCAGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCAAGGCGTCAACTAGCTGCTGTGCTTGATGAATTTTGGGGACAACTGTATGATTTCCATGGGAATGTAATTCAAGATGCAAAGGCTAAAAAGTTGGACCTTTTGTTGGGATCAGATTCAAAGTCTGTGTCGTCCTTGCTGAAAGTTGATACCAGTCCAAAGGAAACTTCTGGATTCTTTCCATCTGCAGGAGGCAGAGGATCTGATTCTCTAATCAGCTCAAGTTTATATGAGTCTGCAAAGCAGCATAGGGTTCAAAGCAGTTTAGAGTCATATGCGGTCCAAAGGGGATCTTCCTCATCGTTGCCTAGCCGCATGCAGTTGTTAGACGCCTATGTGCAAAATTCAAGCCACAGCATTATTGGCTCTGGTGAGAGGCGCTATTCAAGTGTACGCAGTATCCCAACTACTGAAAGTGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATCCCAACTTCTGAGAGTGGTGAGAGGCGCTATTCAAGTGTGCACAGTATCCCAACTTCTGAGAGTTGGGATTACCAGCCAGCCATAGTACACGATTATCAGATTGCATCATATCTCAATCGAATGGAAAATGACAAAAGTTCTGATAACTTGAATGGTCAAATGGAGCCATCAGTCCTACAGTCTGGTTCTTCGTTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAACTGGGTTAGGATCTGGTCAGGCCTCCAGTTTTCAGAACTTTACAGTATCTAGAAATAGTCCAATGCAATCTGAAAGGCCGTATTATGATCCACGGTCTTCTGGAATTGCTGAGAATGTGGTAAGTCCAGCCAATGCAAAGAAATATCATAGTTTACCAGACATTCACCGGGAGCTTTACATGCCTGATAAAAATGCTAACTGGGAAAGTCCTCTTGGGTATGGATCATCTGCTGGGATGACCGGTTATGAATCATCCTTGTATTCAAATTCTGGGGTACGAACAGGAGCTCCTTTGGCATTTGATGAACTCTCTCCAAAAGTCTACAATTCTCTGTCATCACAACAGAGTTCTACTTTCAACACTGGGTCGCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTGGCTGATACTAATCGTACTATTGGTAGTGGAATTGGTAATAGGGTAGGTTCTGTAAATCAAGAAACAACTTCAGTTGCAGATTCAGAGGCGAAGCTTCTACAGTCTTTCAGGCATTGCATTGTGAAGCTTTTGAAATTGGAAGGGTCTGACTGGTTGTTTACGCAAAATGATGGGGTTGATGAGGATCTAATTGATCGTGTGGCTTCAAGGGAAAAATTTCTTTATGAAGCTGAAACTAGAGAGATAAACCGAACAGGTCAAATGGGCGAGCCGCAGTACCATTCTTCAGATAGGAAGCCTGCTTCTGCAATGAAGAATAATGATTTGAGTCAGTTTATGTTTTCCTCGGTTCCTCATTGTGGGGAGGGGTGTATATGGAAGGCAGATTTGGTAGTAAGCTTTGGGGTTTGGTGCATCCACCGCATTCTTGATTTATCACTTATGGAAAGCCGGCCAGAGCTATGGGGGAAATATACCTACGTTCTCAACCGGCTTCAGGGGATTATTGATTCGGCATTTTCAAAGCCTCGTTCTCCGATGTCACCATGCTTCTGCCTTAAAGTTCCCGCTGCACACCAGCTGAAATCAAGTCTATCATTTTCAAATGGAATACCTCCTGCTGCTAAACCAGCCAGGGGAAAATCCACAACTGCAGTAACGCTTCTAGACATAATCAAGGATGTGGAGATTGCAATCTCTTGTCGAAAGGGCCGGACAGGGACAGCAGCCGGTGATGTAGCTTTCCCAAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGTAGATTATCCAACAAAGCCGTTGGCACTCATGAGGGTCCTGGTTCGCGCAAGTCTCTGACATCTGCTCCTTATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1330

Amino Acids

144.3

Weight (kDa)

5.57

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 395 1e-78 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
Acc16I TGCGCA 1 cut(s) 2456
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 134, 1169
AccB7I CCANNNNNTGG 1 cut(s) 1535
AccI GTMKAC 1 cut(s) 3036
AccII CGCG 1 cut(s) 3958
AciI CCGC 9 cut(s) 49, 776, 1305, 1678, 2291, 2326, 3392, 3541, 3687
AcoI YGGCCR 1 cut(s) 3572
AcuI CTGAAG 8 cut(s) 147, 718, 1793, 1836, 1890, 3174, 3390, 3599
AcyI GRCGYC 2 cut(s) 2020, 2343
AfaI GTAC 8 cut(s) 425, 879, 1438, 2406, 2550, 2991, 3136, 3398
AgeI ACCGGT 1 cut(s) 2954
AjnI CCWGG 6 cut(s) 103, 444, 985, 1859, 3752, 3949
AjuI GAANNNNNNNTTGG 8 cut(s) 1248, 1280, 2164, 2196, 2910, 2942, 3491, 3523
AloI GAACNNNNNNTCC 2 cut(s) 2986, 3018
Alw21I GWGCWC 4 cut(s) 1771, 1901, 2505, 3004
Alw26I GTCTC 2 cut(s) 1723, 3969
Alw44I GTGCAC 2 cut(s) 1767, 2501
AlwNI CAGNNNCTG 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 1924
AoxI GGCC 6 cut(s) 811, 1911, 2733, 2789, 3572, 3829
ApaLI GTGCAC 2 cut(s) 1767, 2501
ArsI GACNNNNNNTTYG 2 cut(s) 2126, 2158
AsiGI ACCGGT 1 cut(s) 2954
Asp700I GAANNNNTTC 2 cut(s) 1984, 3336
AspLEI GCGC 7 cut(s) 595, 1004, 2394, 2442, 2457, 2490, 3960
AspS9I GGNCC 8 cut(s) 68, 466, 869, 979, 2110, 2293, 3829, 3947
AsuC2I CCSGG 2 cut(s) 36, 2882
AsuNHI GCTAGC 1 cut(s) 1363
AvaI CYCGRG 1 cut(s) 1924
AvaII GGWCC 7 cut(s) 68, 466, 869, 979, 2110, 2293, 3947
AxyI CCTNAGG 1 cut(s) 289
BaeGI GKGCMC 5 cut(s) 139, 278, 1174, 1771, 2505
BamHI GGATCC 1 cut(s) 1688
BanI GGYRCC 2 cut(s) 134, 1169
BanII GRGCYC 2 cut(s) 1925, 3004
BarI GAAGNNNNNNTAC 6 cut(s) 1809, 1841, 2452, 2484, 2500, 2532
BbsI GAAGAC 3 cut(s) 596, 1808, 2802
Bbv12I GWGCWC 4 cut(s) 1771, 1901, 2505, 3004
BbvCI CCTCAGC 1 cut(s) 772
BccI CCATC 7 cut(s) 1126, 1423, 1537, 1955, 2203, 2638, 3284
BceAI ACGGC 3 cut(s) 392, 2776, 3914
BciT130I CCWGG 6 cut(s) 105, 446, 987, 1861, 3754, 3951
BciVI GTATCC 3 cut(s) 2423, 2471, 2519
BclI TGATCA 1 cut(s) 1603
BcnI CCSGG 2 cut(s) 36, 2882
BcoDI GTCTC 2 cut(s) 1723, 3969
BfaI CTAG 8 cut(s) 1364, 1580, 2027, 2322, 2763, 3093, 3357, 3785
BfoI RGCGCY 3 cut(s) 2395, 2443, 2491
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 3 cut(s) 2423, 2471, 2519
BglII AGATCT 2 cut(s) 238, 1694
Bme1390I CCNGG 8 cut(s) 36, 105, 446, 987, 1861, 2882, 3754, 3951
Bme18I GGWCC 7 cut(s) 68, 466, 869, 979, 2110, 2293, 3947
BmeT110I CYCGRG 1 cut(s) 1924
BmgT120I GGNCC 8 cut(s) 68, 466, 869, 979, 2110, 2293, 3829, 3947
BmiI GGNNCC 9 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2628, 3471, 3948
BmrFI CCNGG 8 cut(s) 36, 105, 446, 987, 1861, 2882, 3754, 3951
BmrI ACTGGG 4 cut(s) 1429, 2724, 2923, 3087
BmtI GCTAGC 1 cut(s) 1367
BmuI ACTGGG 4 cut(s) 1429, 2724, 2923, 3087
BpiI GAAGAC 3 cut(s) 596, 1808, 2802
BpmI CTGGAG 2 cut(s) 955, 2722
Bpu10I CCTNAGC 2 cut(s) 772, 1407
BpuEI CTTGAG 1 cut(s) 2218
BpuMI CCSGG 2 cut(s) 36, 2882
BsaBI GATNNNNATC 5 cut(s) 1575, 1693, 3129, 3303, 3812
BsaHI GRCGYC 2 cut(s) 2020, 2343
BsaJI CCNNGG 9 cut(s) 103, 340, 687, 1860, 2071, 2694, 2804, 3465, 3753
BsaWI WCCGGW 2 cut(s) 230, 2954
BsaXI ACNNNNNCTCC 9 cut(s) 928, 939, 958, 969, 1930, 1960, 2986, 3016, 3961
Bse118I RCCGGY 5 cut(s) 880, 2954, 3570, 3609, 3848
Bse21I CCTNAGG 1 cut(s) 289
Bse3DI GCAATG 5 cut(s) 668, 846, 997, 3232, 3435
Bse8I GATNNNNATC 5 cut(s) 1575, 1693, 3129, 3303, 3812
BseBI CCWGG 6 cut(s) 105, 446, 987, 1861, 3754, 3951
BseDI CCNNGG 9 cut(s) 103, 340, 687, 1860, 2071, 2694, 2804, 3465, 3753
BseGI GGATG 8 cut(s) 564, 841, 1230, 1549, 2955, 2966, 3534, 3805
BseJI GATNNNNATC 5 cut(s) 1575, 1693, 3129, 3303, 3812
BseMI GCAATG 5 cut(s) 668, 846, 997, 3232, 3435
BseMII CTCAG 7 cut(s) 563, 786, 1421, 1908, 2463, 2511, 2814
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 5 cut(s) 139, 278, 1174, 1771, 2505
BseYI CCCAGC 1 cut(s) 2945
BsgI GTGCAG 3 cut(s) 677, 1995, 3675
Bsh1236I CGCG 1 cut(s) 3958
BshFI GGCC 6 cut(s) 813, 1913, 2735, 2791, 3574, 3831
BshNI GGYRCC 2 cut(s) 134, 1169
BshTI ACCGGT 1 cut(s) 2954
BsiHKAI GWGCWC 4 cut(s) 1771, 1901, 2505, 3004
BsiHKCI CYCGRG 1 cut(s) 1924
BsiSI CCGG 9 cut(s) 35, 231, 881, 2881, 2955, 3571, 3610, 3832, 3849
BslFI GGGAC 3 cut(s) 392, 2067, 3853
BsmAI GTCTC 2 cut(s) 1723, 3969
BsmFI GGGAC 3 cut(s) 392, 2067, 3853
BsmI GAATGC 1 cut(s) 3543
BsnI GGCC 6 cut(s) 813, 1913, 2735, 2791, 3574, 3831
BsoBI CYCGRG 1 cut(s) 1924
Bsp1286I GDGCHC 8 cut(s) 139, 278, 1174, 1771, 1901, 1925, 2505, 3004
Bsp19I CCATGG 2 cut(s) 2071, 2694
BspACI CCGC 9 cut(s) 49, 776, 1305, 1678, 2291, 2326, 3392, 3541, 3687
BspANI GGCC 6 cut(s) 813, 1913, 2735, 2791, 3574, 3831
BspCNI CTCAG 7 cut(s) 564, 785, 1420, 1909, 2464, 2512, 2815
BspFNI CGCG 1 cut(s) 3958
BspHI TCATGA 1 cut(s) 3940
BspLI GGNNCC 9 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2628, 3471, 3948
BspMAI CTGCAG 3 cut(s) 1702, 2203, 3775
BspMI ACCTGC 1 cut(s) 122
BspOI GCTAGC 1 cut(s) 1367
BspT107I GGYRCC 2 cut(s) 134, 1169
BsrDI GCAATG 5 cut(s) 668, 846, 997, 3232, 3435
BsrFI RCCGGY 5 cut(s) 880, 2954, 3570, 3609, 3848
BssAI RCCGGY 5 cut(s) 880, 2954, 3570, 3609, 3848
BssECI CCNNGG 9 cut(s) 103, 340, 687, 1860, 2071, 2694, 2804, 3465, 3753
BssNI GRCGYC 2 cut(s) 2020, 2343
BssT1I CCWWGG 3 cut(s) 687, 2071, 2694
Bst2UI CCWGG 6 cut(s) 105, 446, 987, 1861, 3754, 3951
Bst6I CTCTTC 2 cut(s) 229, 1458
BstACI GRCGYC 2 cut(s) 2020, 2343
BstAPI GCANNNNNTGC 2 cut(s) 122, 2707
BstC8I GCNNGC 9 cut(s) 616, 675, 1365, 2010, 2330, 2541, 3389, 3420, 3572
BstDEI CTNAG 9 cut(s) 289, 572, 772, 1407, 1917, 1943, 2472, 2520, 2823
BstDSI CCRYGG 3 cut(s) 2071, 2694, 2804
BstF5I GGATG 8 cut(s) 564, 841, 1230, 1549, 2955, 2966, 3534, 3805
BstFNI CGCG 1 cut(s) 3958
BstH2I RGCGCY 3 cut(s) 2395, 2443, 2491
BstHHI GCGC 7 cut(s) 595, 1004, 2394, 2442, 2457, 2490, 3960
BstMAI GTCTC 2 cut(s) 1723, 3969
BstNI CCWGG 6 cut(s) 105, 446, 987, 1861, 3754, 3951
BstNSI RCATGY 3 cut(s) 282, 2332, 2896
BstSCI CCNGG 8 cut(s) 34, 103, 444, 985, 1859, 2880, 3752, 3949
BstSLI GKGCMC 5 cut(s) 139, 278, 1174, 1771, 2505
BstUI CGCG 1 cut(s) 3958
BstV2I GAAGAC 3 cut(s) 596, 1808, 2802
BstX2I RGATCY 8 cut(s) 238, 1576, 1688, 1694, 2213, 2303, 2723, 3304
BstXI CCANNNNNNTGG 1 cut(s) 3582
BstYI RGATCY 8 cut(s) 238, 1576, 1688, 1694, 2213, 2303, 2723, 3304
Bsu36I CCTNAGG 1 cut(s) 289
BsuI GTATCC 3 cut(s) 2423, 2471, 2519
BsuRI GGCC 6 cut(s) 813, 1913, 2735, 2791, 3574, 3831
BtgI CCRYGG 3 cut(s) 2071, 2694, 2804
BtgZI GCGATG 1 cut(s) 331
BtsCI GGATG 8 cut(s) 564, 841, 1230, 1549, 2955, 2966, 3534, 3805
BtsI GCAGTG 3 cut(s) 262, 1329, 1707
BtsIMutI CAGTG 8 cut(s) 262, 742, 1329, 1707, 1769, 1771, 1899, 3075
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 9 cut(s) 616, 675, 1365, 2010, 2330, 2541, 3389, 3420, 3572
CaiI CAGNNNCTG 1 cut(s) 122
CciI TCATGA 1 cut(s) 3940
CfoI GCGC 7 cut(s) 595, 1004, 2394, 2442, 2457, 2490, 3960
Cfr10I RCCGGY 5 cut(s) 880, 2954, 3570, 3609, 3848
Cfr13I GGNCC 8 cut(s) 68, 466, 869, 979, 2110, 2293, 3829, 3947
CseI GACGC 2 cut(s) 2009, 2351
Csp6I GTAC 8 cut(s) 424, 878, 1437, 2405, 2549, 2990, 3135, 3397
CspAI ACCGGT 1 cut(s) 2954
CviQI GTAC 8 cut(s) 424, 878, 1437, 2405, 2549, 2990, 3135, 3397
DdeI CTNAG 9 cut(s) 289, 572, 772, 1407, 1917, 1943, 2472, 2520, 2823
EaeI YGGCCR 1 cut(s) 3572
Eam1104I CTCTTC 2 cut(s) 229, 1458
EarI CTCTTC 2 cut(s) 229, 1458
Ecl136II GAGCTC 1 cut(s) 3002
Eco130I CCWWGG 3 cut(s) 687, 2071, 2694
Eco147I AGGCCT 1 cut(s) 2735
Eco24I GRGCYC 2 cut(s) 1925, 3004
Eco47I GGWCC 7 cut(s) 68, 466, 869, 979, 2110, 2293, 3947
Eco53kI GAGCTC 1 cut(s) 3002
Eco57I CTGAAG 8 cut(s) 147, 718, 1793, 1836, 1890, 3174, 3390, 3599
Eco81I CCTNAGG 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 1924
EcoICRI GAGCTC 1 cut(s) 3002
EcoO109I RGGNCCY 2 cut(s) 466, 3947
EcoRI GAATTC 2 cut(s) 294, 1196
EcoRII CCWGG 6 cut(s) 103, 444, 985, 1859, 3752, 3949
EcoT14I CCWWGG 3 cut(s) 687, 2071, 2694
EcoT22I ATGCAT 3 cut(s) 284, 476, 731
EcoT38I GRGCYC 2 cut(s) 1925, 3004
ErhI CCWWGG 3 cut(s) 687, 2071, 2694
FalI AAGNNNNNCTT 4 cut(s) 966, 998, 2290, 2322
FaqI GGGAC 3 cut(s) 392, 2067, 3853
FauI CCCGC 2 cut(s) 1298, 3694
FauNDI CATATG 1 cut(s) 2287
FbaI TGATCA 1 cut(s) 1603
FblI GTMKAC 1 cut(s) 3036
FokI GGATG 8 cut(s) 571, 848, 1237, 1556, 2953, 2962, 3521, 3812
FriOI GRGCYC 2 cut(s) 1925, 3004
FspBI CTAG 8 cut(s) 1364, 1580, 2027, 2322, 2763, 3093, 3357, 3785
FspI TGCGCA 1 cut(s) 2456
GlaI GCGC 7 cut(s) 594, 1003, 2393, 2441, 2456, 2489, 3959
GsaI CCCAGC 1 cut(s) 2949
GsuI CTGGAG 2 cut(s) 955, 2722
HaeII RGCGCY 3 cut(s) 2395, 2443, 2491
HaeIII GGCC 6 cut(s) 813, 1913, 2735, 2791, 3574, 3831
HapII CCGG 9 cut(s) 35, 231, 881, 2881, 2955, 3571, 3610, 3832, 3849
HgaI GACGC 2 cut(s) 2009, 2351
HhaI GCGC 7 cut(s) 595, 1004, 2394, 2442, 2457, 2490, 3960
Hin1I GRCGYC 2 cut(s) 2020, 2343
Hin6I GCGC 7 cut(s) 593, 1002, 2392, 2440, 2455, 2488, 3958
HinP1I GCGC 7 cut(s) 593, 1002, 2392, 2440, 2455, 2488, 3958
HincII GTYRAC 3 cut(s) 530, 1785, 2024
HindII GTYRAC 3 cut(s) 530, 1785, 2024
HindIII AAGCTT 3 cut(s) 3212, 3245, 3517
HpaII CCGG 9 cut(s) 35, 231, 881, 2881, 2955, 3571, 3610, 3832, 3849
Hpy99I CGWCG 1 cut(s) 1961
HpyAV CCTTC 7 cut(s) 451, 457, 898, 992, 1058, 3254, 3493
HpyCH4IV ACGT 2 cut(s) 827, 3600
HpyF3I CTNAG 9 cut(s) 289, 572, 772, 1407, 1917, 1943, 2472, 2520, 2823
HpySE526I ACGT 2 cut(s) 827, 3600
Hsp92I GRCGYC 2 cut(s) 2020, 2343
HspAI GCGC 7 cut(s) 593, 1002, 2392, 2440, 2455, 2488, 3958
KroI GCCGGC 1 cut(s) 3570
KroNI GCCGGC 1 cut(s) 3572
Ksp22I TGATCA 1 cut(s) 1603
LmnI GCTCC 9 cut(s) 1058, 1670, 1864, 1896, 2626, 2884, 2999, 3007, 3982
MaeI CTAG 8 cut(s) 1364, 1580, 2027, 2322, 2763, 3093, 3357, 3785
MaeII ACGT 2 cut(s) 827, 3600
MaeIII GTNAC 7 cut(s) 197, 226, 1317, 1524, 1747, 3660, 3775
MfeI CAATTG 1 cut(s) 1794
MflI RGATCY 8 cut(s) 238, 1576, 1688, 1694, 2213, 2303, 2723, 3304
MhlI GDGCHC 8 cut(s) 139, 278, 1174, 1771, 1901, 1925, 2505, 3004
MlyI GAGTC 8 cut(s) 610, 946, 1715, 1799, 1948, 2255, 2291, 3457
MmeI TCCRAC 5 cut(s) 46, 70, 1521, 2088, 3945
Mph1103I ATGCAT 3 cut(s) 284, 476, 731
MroNI GCCGGC 1 cut(s) 3570
MroXI GAANNNNTTC 2 cut(s) 1984, 3336
MseI TTAA 8 cut(s) 360, 411, 929, 1292, 1379, 1470, 1731, 3678
MslI CAYNNNNRTG 2 cut(s) 250, 2076
MspA1I CMGCKG 4 cut(s) 49, 776, 3689, 3700
MspI CCGG 9 cut(s) 35, 231, 881, 2881, 2955, 3571, 3610, 3832, 3849
MspR9I CCNGG 8 cut(s) 36, 105, 446, 987, 1861, 2882, 3754, 3951
MunI CAATTG 1 cut(s) 1794
Mva1269I GAATGC 1 cut(s) 3543
MvaI CCWGG 6 cut(s) 105, 446, 987, 1861, 3754, 3951
MvnI CGCG 1 cut(s) 3958
NaeI GCCGGC 1 cut(s) 3572
NciI CCSGG 2 cut(s) 36, 2882
NcoI CCATGG 2 cut(s) 2071, 2694
NdeI CATATG 1 cut(s) 2287
NgoMIV GCCGGC 1 cut(s) 3570
NheI GCTAGC 1 cut(s) 1363
NlaIV GGNNCC 9 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2628, 3471, 3948
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 5 cut(s) 197, 226, 1524, 1747, 3660
NsbI TGCGCA 1 cut(s) 2456
NsiI ATGCAT 3 cut(s) 284, 476, 731
NspI RCATGY 3 cut(s) 282, 2332, 2896
PaeI GCATGC 1 cut(s) 2332
PagI TCATGA 1 cut(s) 3940
PaqCI CACCTGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 2735
PcsI WCGNNNNNNNCGW 1 cut(s) 3907
PctI GAATGC 1 cut(s) 3543
PdiI GCCGGC 1 cut(s) 3572
PdmI GAANNNNTTC 2 cut(s) 1984, 3336
PflMI CCANNNNNTGG 1 cut(s) 1535
PfoI TCCNGGA 1 cut(s) 444
PinAI ACCGGT 1 cut(s) 2954
PleI GAGTC 8 cut(s) 609, 945, 1714, 1798, 1947, 2254, 2290, 3456
PpsI GAGTC 8 cut(s) 609, 945, 1714, 1798, 1947, 2254, 2290, 3456
PpuMI RGGWCCY 2 cut(s) 466, 3947
Psp124BI GAGCTC 1 cut(s) 3004
Psp5II RGGWCCY 2 cut(s) 466, 3947
Psp6I CCWGG 6 cut(s) 103, 444, 985, 1859, 3752, 3949
PspFI CCCAGC 1 cut(s) 2945
PspGI CCWGG 6 cut(s) 103, 444, 985, 1859, 3752, 3949
PspN4I GGNNCC 9 cut(s) 136, 870, 1060, 1171, 1690, 1866, 2628, 3471, 3948
PspPI GGNCC 8 cut(s) 68, 466, 869, 979, 2110, 2293, 3829, 3947
PspPPI RGGWCCY 2 cut(s) 466, 3947
PstI CTGCAG 3 cut(s) 1702, 2203, 3775
PstNI CAGNNNCTG 1 cut(s) 122
PsuI RGATCY 8 cut(s) 238, 1576, 1688, 1694, 2213, 2303, 2723, 3304
PvuII CAGCTG 1 cut(s) 3700
RsaI GTAC 8 cut(s) 425, 879, 1438, 2406, 2550, 2991, 3136, 3398
RsaNI GTAC 8 cut(s) 424, 878, 1437, 2405, 2549, 2990, 3135, 3397
RseI CAYNNNNRTG 2 cut(s) 250, 2076
SacI GAGCTC 1 cut(s) 3004
SaqAI TTAA 8 cut(s) 360, 411, 929, 1292, 1379, 1470, 1731, 3678
Sau96I GGNCC 8 cut(s) 68, 466, 869, 979, 2110, 2293, 3829, 3947
SchI GAGTC 8 cut(s) 610, 946, 1715, 1799, 1948, 2255, 2291, 3457
ScrFI CCNGG 8 cut(s) 36, 105, 446, 987, 1861, 2882, 3754, 3951
SduI GDGCHC 8 cut(s) 139, 278, 1174, 1771, 1901, 1925, 2505, 3004
SinI GGWCC 7 cut(s) 68, 466, 869, 979, 2110, 2293, 3947
SmiMI CAYNNNNRTG 2 cut(s) 250, 2076
SmlI CTYRAG 1 cut(s) 2233
SmoI CTYRAG 1 cut(s) 2233
SphI GCATGC 1 cut(s) 2332
SseBI AGGCCT 1 cut(s) 2735
SsiI CCGC 9 cut(s) 49, 776, 1305, 1678, 2291, 2326, 3392, 3541, 3687
SspMI CTAG 8 cut(s) 1364, 1580, 2027, 2322, 2763, 3093, 3357, 3785
SstI GAGCTC 1 cut(s) 3004
StuI AGGCCT 1 cut(s) 2735
StyD4I CCNGG 8 cut(s) 34, 103, 444, 985, 1859, 2880, 3752, 3949
StyI CCWWGG 3 cut(s) 687, 2071, 2694
TaiI ACGT 2 cut(s) 830, 3603
TaqI TCGA 3 cut(s) 1298, 2581, 3823
TatI WGTACW 1 cut(s) 2548
TauI GCSGC 3 cut(s) 1680, 2328, 3394
Tru1I TTAA 8 cut(s) 360, 411, 929, 1292, 1379, 1470, 1731, 3678
Tru9I TTAA 8 cut(s) 360, 411, 929, 1292, 1379, 1470, 1731, 3678
TscAI CASTG 8 cut(s) 262, 742, 1336, 1707, 1771, 1776, 1906, 3082
TseFI GTSAC 5 cut(s) 197, 226, 1524, 1747, 3660
Tsp45I GTSAC 5 cut(s) 197, 226, 1524, 1747, 3660
TspRI CASTG 8 cut(s) 262, 742, 1336, 1707, 1771, 1776, 1906, 3082
Van91I CCANNNNNTGG 1 cut(s) 1535
VneI GTGCAC 2 cut(s) 1767, 2501
VpaK11BI GGWCC 7 cut(s) 68, 466, 869, 979, 2110, 2293, 3947
XbaI TCTAGA 3 cut(s) 2762, 3092, 3784
XceI RCATGY 3 cut(s) 282, 2332, 2896
XcmI CCANNNNNNNNNTGG 2 cut(s) 1699, 2372
XmiI GTMKAC 1 cut(s) 3036
XmnI GAANNNNTTC 2 cut(s) 1984, 3336
XspI CTAG 8 cut(s) 1364, 1580, 2027, 2322, 2763, 3093, 3357, 3785
Zsp2I ATGCAT 3 cut(s) 284, 476, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.