RLG00000024319

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002662
Physical Location & Seq
Reverse (-)
34531357 .. 34537498
6142 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024319

Sequence Viewer

Length: 4065 bp
ATGGAATCTGCTATCTCTAACACTAACAATATGCCAGGCGTTGTCCATCAGTTGCTTCCTGTTGTTGGACCCATGCTTCTGATTGCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGTTGAAGCAGGTTCCCGTTATGGAACTGATCTGGCTGCAGTAATGCTTATATTCAATTTGGCTGCTATTTTATGTCACTATCTGTCAGCGCGGATTGCTGTAGTCACTGGAAGAGATCTTGCTCAGGTTTGCAGTGAGGAGTATGACAAGGCTACGTGCATATTCTTAGGAGTACAAACAGAGATGTCGGTGATTGTGTTGGACCTTACCATGATCCTTGGCATCGCACATGGACTTAATCTTCTGTTTGGGTGGGACTTGTTCACTTGTGTGTTTTTGACTGCTGCTAATGCTGTTTTATACCCTCTTTTTTCCACCCTCCTGGAGACTTGCAAGGCAAAATTCCTTTGCGTATGCATATACATTGCAGGATTTCTACTGCTTTCCTTTGTTCTTGGAGTATTTATCAGTCAACCACAAGTGCCACTTTCCATGACTGGGATGTTAACAAAACTGAGTGGGGAAAGTGCTTTTTCACTGATGAGTCTTCTTGGAGCAAGTATAATGCCCCACAGCTTTTATCTTCATTCTTCGATTGTGCAGCAGCATCAGCAACAACCAACTGTTTCTAAGGATGCCTTGTGTCAGAACCATTTTGTTGCCATCTTCTGCACCTTTAGTGGTATTTATCTGGTGAATTATGCCCTCATGACCTTAGCAGCAAATGTATTCTACACTTCACGTGGTTTGCTTACATTTCAGGATGCAATGTCCCTAATGGAACAGGTCTTTTGGGGTCCAATAGTACCTGTTGCCTTCTTGCTAGTTCTCTTTTTATCAAATCAAATCACAACATTAAGCTGGAGTTTGGGGGGACAAGTAGTCTTGAATGATTTTTTAAAACTAGACCTTCCTGGTTGGCTTCACTGTGCTACAATCAGAATCATTGCCATTGTTCCTGCTCTGTATTTTGTTTGGAGCTCAGGAGCCGAGGGGATGTACCAACTGCTTATATCTACACAGGTGTTGGCAGCCTTGCTACTGCCGTCTTCTGTGATCCCTCTTTTTCGTGTTGCTGCTTCAAGACAAATAATGGGAGCCCATAAGATCTCTCAGTTCGTTGAATTCTTAGCCCTCATTACACTTATTGGGATGCTTGGATTAAAACTTGTCTTTGTAGTGGAAATGATTTTTGGGAATAGTGATTGGGTGGATAATTTGAGATGGGATGCTGGGAGTAGTATGTCTGCACTTCTCATCACTGCTTCTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACACCGTTAAAATCTGCAAGTGCTCGATTAGAGAACCAGGTGTGGAACTGGGATATGCCTAAGGGTGTATCTGAGCCATTTAGAAGTAAGGAGGAGATTGATATAGCTGAGCCTAATTATCATAGAGATGCAAGTGTTCAGAAGCATGAACCATCACCATCTTCTGGGAAGGCTTTGGATAGAGATTCAGATACAGCGGTTGCAAATTTTGATTTTGTTCTGCCCGAAACTCTCTTGGAGCCTGATGAGGAGCTTCAATCAACTACCTCAGAGGAAAATAGTTCTCTTAATACCTTTCCTCGCTCCGCCAAATGCAGTAAGGAGGAACCCACATCTGTGGTGGAGTCAATTTGCCTCCCAACTTTGGCTAGTGAGGTTTCTGATGTTTCATCGCTGCGCACCAGTACTGTGAAAGTTGAATCAACAGAGCCAGTAGAGAAGACTCTTGAAGTTGAAGGAGACTTACCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAACCTGAAGATTCCTTGAAAGAGGTTTCTGGGGGCACCACTTCATTGACATCTGAGGGTCCAGGATCATTCAGGAGTCTCAGTGGAAAAGGTGATGAAGGGGGGAGTGGTGCTGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCTAGACGTCAACTAGCTGCAGTACTCGATGAGTTTTGGGGACAACTGTATGATTTCCATGGTAATGTAATTCAAGAAGCAAAGGCTAGGAAACTGGATCTGTTGTTGGGGTCAGATTCAAAGGCTTCTTCTGCTGCTTCCTCCGCTTCCTCCGTGCTGAAAGATGATACCAATGCAAAGGAAGTTTCTGGATACTTTCCATCTGTAGGAGGCAGAGGATCTGATCCTTTAATCAACTCAAGTTTATACGACTCCGTAAATCAACCGAGGCTGCAAAACAGTTTAGAGTCATCATATGGGGCTCAAAGGGGATCTTCCTCATTATGGTCTGGCCACATACCGTTGCTGGATGCGTATGTACAAAATTCAACCCGCAGTATTATTGACTCAGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCATCTTCAGACTTGGGTGAGAGGCTCTATTCAAGTGTGTGCAGTATACCATCTTCTGACTCGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCAACTTCTGACTCGGGTGAGAGGCGCTATTCAAGTGTCCGCAGCATACCATCTGCTGAGAGCTGGGATTACCAGCCAGCCACAGTACACGGTTATCAGTTTTCATCGTATCTTAATCGTAATGACAGAAGTTCTGGTAACTTGAATGGTCAAATGGAGTCACCAGCCCTAAATTCTGCTTCTTCATTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGCTCTGGACAGGTCTCCAGTTTTCAGAACCTTACAGTATCTCGACATAGTCCGTTGCAATCTGAAAGACCATATTATGATGTACACTCTTCTGGACTTTCTGAGAATGTGGTAAATTCAGCCAATGCAAAGAAATACCACAGTTTACCTGACATTCACCGCGATCTCTACATGTCTAACAAAAGTGCTCAACGGGATGCGCCCCCTGGGTTTGGGAAAACTAATTATGAATCATCCTTGTATCCAAAATCTGGTGCACGGACAGGAGGTCCTTTGGCATTTGATGAACTCTCTCCATCAAAAGTCTATAGAGATGTTCTATCATCCCAACAGAATTCTAATTTTGGCACTGGATCCCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCGTTCTATTGGGACTGCAGTTGGTGGTAGAGCAGGCTCTGCAGGTCAGGAAGCTATGTCAGTTGCAGATTCAGAGGCGAAGCTTCTTCAGTCTTTTAGACACTGCATTGTGAAGCTTTTGAAGTTGGAAGGGTCTGACTGGTTGTTTAGACAGAATGATGGAGTGGATGAGGATCTAATAGATCGTGTGGCTGCCAGGGAGAAAATTCTTTATGAAGCTGAAACTAGAGAGATTAATCGAACAGTTCACATGGGTGAATCTCAGTACACTTCTGACAGAAAGTCAACTTCTGCAATAAAAATGAATGATGCAAATCTCACTCATCTCATGGTTTCCTCAGTTCCTAATTGTGGGGAAGGTTGTATCTGGAGATCTGATCTAATAATAAGCTTTGGGGTCTGGTGTATCCATCGGATTCTTGATTTGTCCCTTATGGAAAGCCGGCCAGAGCTCTGGGGGAAATATACTTACGTTCTCAATCGACTTCAGGGGATTATTGATTCTGCGTTTTCAAAGCCTCGTTCTCCAATGTCTCCATGCTTCTGCCTTCAAATTGCAGCAGCACAGCAGCAGAAGTCTAGTCCAACATTTTCAAATGGAATGTTACCCCCTGCTGCAAAACCAGCCAGGGGAAAATGCACTACAGCTGTAACGCTCCTGGACATAATCAAGGATGTGGAGATTGCAATCTCTTGTCGAAAGGGCCGAACGGGGACAGCAGCCGGTGATGTGGCTTTCCCGAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGGCGATTATCCAACAAACCTGTTGGCACTCAGGAGGGGCCTTCCGGTTCACGAAAGGGTGCTCCAACATCTGCTCCTTATGGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1355

Amino Acids

147.01

Weight (kDa)

5.77

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 392 8.7e-84 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2026
Acc16I TGCGCA 3 cut(s) 1763, 2432, 2528
Acc36I ACCTGC 2 cut(s) 122, 3224
AccB1I GGYRCC 1 cut(s) 1901
AccB7I CCANNNNNTGG 2 cut(s) 1529, 3038
AccI GTMKAC 3 cut(s) 2437, 2485, 2533
AccII CGCG 2 cut(s) 214, 2949
AciI CCGC 9 cut(s) 116, 214, 1562, 1671, 2160, 2389, 2575, 2947, 3976
AcoI YGGCCR 2 cut(s) 2347, 3635
AcsI RAATTY 8 cut(s) 464, 1187, 1570, 2380, 2707, 2902, 3121, 3396
AcuI CTGAAG 4 cut(s) 1893, 2430, 3263, 3662
AcvI CACGTG 1 cut(s) 806
AcyI GRCGYC 1 cut(s) 2023
AfaI GTAC 9 cut(s) 297, 870, 1064, 1771, 2040, 2376, 2622, 2871, 3458
AflIII ACRYGT 1 cut(s) 2958
AhdI GACNNNNNGTC 3 cut(s) 944, 3054, 3472
AjuI GAANNNNNNNTTGG 2 cut(s) 1239, 1271
AleI CACNNNNGTG 3 cut(s) 392, 1700, 3444
Alw21I GWGCWC 6 cut(s) 1046, 1390, 2977, 3046, 3645, 4039
Alw26I GTCTC 5 cut(s) 443, 1818, 1949, 2806, 3729
Alw44I GTGCAC 1 cut(s) 3042
AlwNI CAGNNNCTG 1 cut(s) 3230
Ama87I CYCGRG 2 cut(s) 2500, 2548
AoxI GGCC 4 cut(s) 2347, 3635, 3895, 4013
ApaLI GTGCAC 1 cut(s) 3042
ApoI RAATTY 8 cut(s) 464, 1187, 1570, 2380, 2707, 2902, 3121, 3396
AseI ATTAAT 1 cut(s) 3426
Asp700I GAANNNNTTC 1 cut(s) 1987
AspLEI GCGC 8 cut(s) 214, 1764, 2418, 2433, 2514, 2529, 2562, 2989
AspS9I GGNCC 7 cut(s) 68, 325, 860, 1925, 3056, 3895, 4013
AvaI CYCGRG 2 cut(s) 2500, 2548
AvaII GGWCC 5 cut(s) 68, 325, 860, 1925, 3056
AxyI CCTNAGG 1 cut(s) 1425
BaeGI GKGCMC 2 cut(s) 1904, 3046
BaeI ACNNNNGTAYC 2 cut(s) 1548, 1581
BalI TGGCCA 1 cut(s) 2349
BamHI GGATCC 1 cut(s) 3140
BanI GGYRCC 1 cut(s) 1901
BanII GRGCYC 4 cut(s) 1046, 1165, 2320, 3645
BarI GAAGNNNNNNTAC 8 cut(s) 1812, 1844, 2428, 2460, 2476, 2508, 2524, 2556
BbrPI CACGTG 1 cut(s) 806
BbsI GAAGAC 3 cut(s) 602, 1104, 1811
Bbv12I GWGCWC 6 cut(s) 1046, 1390, 2977, 3046, 3645, 4039
BceAI ACGGC 1 cut(s) 1093
BciVI GTATCC 3 cut(s) 2201, 3039, 3608
BcoDI GTCTC 5 cut(s) 443, 1818, 1949, 2806, 3729
BfaI CTAG 9 cut(s) 887, 968, 1734, 2019, 2030, 2103, 3153, 3417, 3771
BfmI CTRYAG 9 cut(s) 159, 222, 2034, 2220, 2737, 3094, 3207, 3231, 3834
BfoI RGCGCY 3 cut(s) 2419, 2515, 2563
BfuAI ACCTGC 2 cut(s) 122, 3224
BfuI GTATCC 3 cut(s) 2201, 3039, 3608
BglII AGATCT 3 cut(s) 238, 1170, 3563
BlpI GCTNAGC 1 cut(s) 1473
BmcAI AGTACT 2 cut(s) 1771, 2040
Bme18I GGWCC 5 cut(s) 68, 325, 860, 1925, 3056
BmeRI GACNNNNNGTC 3 cut(s) 944, 3054, 3472
BmeT110I CYCGRG 2 cut(s) 2500, 2548
BmgT120I GGNCC 7 cut(s) 68, 325, 860, 1925, 3056, 3895, 4013
BmrI ACTGGG 2 cut(s) 570, 1423
BmuI ACTGGG 2 cut(s) 570, 1423
BpiI GAAGAC 3 cut(s) 602, 1104, 1811
BpmI CTGGAG 4 cut(s) 467, 946, 2788, 3580
Bpu10I CCTNAGC 3 cut(s) 246, 778, 1045
Bpu1102I GCTNAGC 1 cut(s) 1473
BpuEI CTTGAG 1 cut(s) 2239
BsaAI YACGTR 2 cut(s) 279, 806
BsaBI GATNNNNATC 4 cut(s) 3189, 3363, 3504, 3878
BsaHI GRCGYC 1 cut(s) 2023
BsaI GGTCTC 1 cut(s) 2806
BsaWI WCCGGW 1 cut(s) 4019
BsaXI ACNNNNNCTCC 4 cut(s) 1933, 1963, 4033, 4063
Bse118I RCCGGY 2 cut(s) 3633, 3914
Bse1I ACTGG 9 cut(s) 235, 565, 1418, 1767, 1796, 2115, 2805, 3142, 3335
Bse21I CCTNAGG 1 cut(s) 1425
Bse3DI GCAATG 3 cut(s) 486, 837, 1008
Bse8I GATNNNNATC 4 cut(s) 3189, 3363, 3504, 3878
BseJI GATNNNNATC 4 cut(s) 3189, 3363, 3504, 3878
BseMI GCAATG 3 cut(s) 486, 837, 1008
BseNI ACTGG 9 cut(s) 235, 565, 1418, 1767, 1796, 2115, 2805, 3142, 3335
BseRI GAGGAG 3 cut(s) 275, 1472, 1628
BseSI GKGCMC 2 cut(s) 1904, 3046
BseYI CCCAGC 2 cut(s) 1295, 2598
BsgI GTGCAG 4 cut(s) 683, 718, 1296, 2500
Bsh1236I CGCG 2 cut(s) 214, 2949
BshFI GGCC 4 cut(s) 2349, 3637, 3897, 4015
BshNI GGYRCC 1 cut(s) 1901
BsiHKAI GWGCWC 6 cut(s) 1046, 1390, 2977, 3046, 3645, 4039
BsiHKCI CYCGRG 2 cut(s) 2500, 2548
BsiSI CCGG 3 cut(s) 3634, 3915, 4020
BslFI GGGAC 7 cut(s) 392, 820, 951, 2070, 3217, 3604, 3919
BsmAI GTCTC 5 cut(s) 443, 1818, 1949, 2806, 3729
BsmFI GGGAC 7 cut(s) 392, 820, 951, 2070, 3217, 3604, 3919
BsnI GGCC 4 cut(s) 2349, 3637, 3897, 4015
Bso31I GGTCTC 1 cut(s) 2806
BsoBI CYCGRG 2 cut(s) 2500, 2548
Bsp1286I GDGCHC 9 cut(s) 1046, 1165, 1390, 1904, 2320, 2977, 3046, 3645, 4039
Bsp1407I TGTACA 2 cut(s) 2374, 2869
Bsp1720I GCTNAGC 1 cut(s) 1473
Bsp19I CCATGG 2 cut(s) 2074, 2760
BspACI CCGC 9 cut(s) 116, 214, 1562, 1671, 2160, 2389, 2575, 2947, 3976
BspANI GGCC 4 cut(s) 2349, 3637, 3897, 4015
BspFNI CGCG 2 cut(s) 214, 2949
BspHI TCATGA 1 cut(s) 771
BspMAI CTGCAG 4 cut(s) 163, 2038, 3211, 3235
BspMI ACCTGC 2 cut(s) 122, 3224
BspT107I GGYRCC 1 cut(s) 1901
BspTNI GGTCTC 1 cut(s) 2806
BsrDI GCAATG 3 cut(s) 486, 837, 1008
BsrFI RCCGGY 2 cut(s) 3633, 3914
BsrGI TGTACA 2 cut(s) 2374, 2869
BsrI ACTGG 9 cut(s) 235, 565, 1418, 1767, 1796, 2115, 2805, 3142, 3335
BssAI RCCGGY 2 cut(s) 3633, 3914
BssNAI GTATAC 3 cut(s) 2438, 2486, 2534
BssNI GRCGYC 1 cut(s) 2023
BssT1I CCWWGG 3 cut(s) 340, 2074, 2760
Bst1107I GTATAC 3 cut(s) 2438, 2486, 2534
Bst6I CTCTTC 2 cut(s) 229, 2881
BstACI GRCGYC 1 cut(s) 2023
BstAPI GCANNNNNTGC 2 cut(s) 2773, 3230
BstAUI TGTACA 2 cut(s) 2374, 2869
BstBAI YACGTR 2 cut(s) 279, 806
BstC8I GCNNGC 5 cut(s) 1359, 2013, 2613, 3226, 3635
BstDSI CCRYGG 2 cut(s) 2074, 2760
BstFNI CGCG 2 cut(s) 214, 2949
BstH2I RGCGCY 3 cut(s) 2419, 2515, 2563
BstHHI GCGC 8 cut(s) 214, 1764, 2418, 2433, 2514, 2529, 2562, 2989
BstMAI GTCTC 5 cut(s) 443, 1818, 1949, 2806, 3729
BstMWI GCNNNNNNNGC 9 cut(s) 218, 413, 673, 2147, 2159, 2773, 3166, 3230, 3815
BstNSI RCATGY 1 cut(s) 2962
BstSFI CTRYAG 9 cut(s) 159, 222, 2034, 2220, 2737, 3094, 3207, 3231, 3834
BstSLI GKGCMC 2 cut(s) 1904, 3046
BstUI CGCG 2 cut(s) 214, 2949
BstV2I GAAGAC 3 cut(s) 602, 1104, 1811
BstX2I RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3140, 3364, 3563
BstXI CCANNNNNNTGG 3 cut(s) 445, 1702, 3645
BstYI RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3140, 3364, 3563
BstZ17I GTATAC 3 cut(s) 2438, 2486, 2534
Bsu36I CCTNAGG 1 cut(s) 1425
BsuI GTATCC 3 cut(s) 2201, 3039, 3608
BsuRI GGCC 4 cut(s) 2349, 3637, 3897, 4015
BtgI CCRYGG 2 cut(s) 2074, 2760
BtgZI GCGATG 2 cut(s) 331, 1740
BtsI GCAGTG 3 cut(s) 262, 1323, 3292
BtsIMutI CAGTG 8 cut(s) 228, 262, 599, 988, 1323, 1954, 3135, 3292
BveI ACCTGC 2 cut(s) 122, 3224
Cac8I GCNNGC 5 cut(s) 1359, 2013, 2613, 3226, 3635
CaiI CAGNNNCTG 1 cut(s) 3230
CciI TCATGA 1 cut(s) 771
CfoI GCGC 8 cut(s) 214, 1764, 2418, 2433, 2514, 2529, 2562, 2989
Cfr10I RCCGGY 2 cut(s) 3633, 3914
Cfr13I GGNCC 7 cut(s) 68, 325, 860, 1925, 3056, 3895, 4013
CsiI ACCWGGT 1 cut(s) 1401
Csp6I GTAC 9 cut(s) 296, 869, 1063, 1770, 2039, 2375, 2621, 2870, 3457
CviQI GTAC 9 cut(s) 296, 869, 1063, 1770, 2039, 2375, 2621, 2870, 3457
DraI TTTAAA 1 cut(s) 963
DriI GACNNNNNGTC 3 cut(s) 944, 3054, 3472
EaeI YGGCCR 2 cut(s) 2347, 3635
Eam1104I CTCTTC 2 cut(s) 229, 2881
Eam1105I GACNNNNNGTC 3 cut(s) 944, 3054, 3472
EarI CTCTTC 2 cut(s) 229, 2881
EciI GGCGGA 1 cut(s) 1660
Ecl136II GAGCTC 2 cut(s) 1044, 3643
Eco130I CCWWGG 3 cut(s) 340, 2074, 2760
Eco24I GRGCYC 4 cut(s) 1046, 1165, 2320, 3645
Eco31I GGTCTC 1 cut(s) 2806
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 5 cut(s) 68, 325, 860, 1925, 3056
Eco53kI GAGCTC 2 cut(s) 1044, 3643
Eco57I CTGAAG 4 cut(s) 1893, 2430, 3263, 3662
Eco72I CACGTG 1 cut(s) 806
Eco81I CCTNAGG 1 cut(s) 1425
Eco88I CYCGRG 2 cut(s) 2500, 2548
EcoICRI GAGCTC 2 cut(s) 1044, 3643
EcoO109I RGGNCCY 2 cut(s) 3056, 4013
EcoRI GAATTC 2 cut(s) 1187, 3121
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 340, 2074, 2760
EcoT22I ATGCAT 1 cut(s) 482
EcoT38I GRGCYC 4 cut(s) 1046, 1165, 2320, 3645
ErhI CCWWGG 3 cut(s) 340, 2074, 2760
FalI AAGNNNNNCTT 6 cut(s) 534, 566, 686, 718, 2314, 2346
FaqI GGGAC 7 cut(s) 392, 820, 951, 2070, 3217, 3604, 3919
FauI CCCGC 1 cut(s) 2396
FauNDI CATATG 1 cut(s) 2311
FblI GTMKAC 3 cut(s) 2437, 2485, 2533
FriOI GRGCYC 4 cut(s) 1046, 1165, 2320, 3645
FspBI CTAG 9 cut(s) 887, 968, 1734, 2019, 2030, 2103, 3153, 3417, 3771
FspI TGCGCA 3 cut(s) 1763, 2432, 2528
GlaI GCGC 8 cut(s) 213, 1763, 2417, 2432, 2513, 2528, 2561, 2988
GsaI CCCAGC 2 cut(s) 1299, 2602
GsuI CTGGAG 4 cut(s) 467, 946, 2788, 3580
HaeII RGCGCY 3 cut(s) 2419, 2515, 2563
HaeIII GGCC 4 cut(s) 2349, 3637, 3897, 4015
HapII CCGG 3 cut(s) 3634, 3915, 4020
HhaI GCGC 8 cut(s) 214, 1764, 2418, 2433, 2514, 2529, 2562, 2989
Hin1I GRCGYC 1 cut(s) 2023
Hin6I GCGC 8 cut(s) 212, 1762, 2416, 2431, 2512, 2527, 2560, 2987
HinP1I GCGC 8 cut(s) 212, 1762, 2416, 2431, 2512, 2527, 2560, 2987
HincII GTYRAC 4 cut(s) 536, 570, 2027, 3477
HindII GTYRAC 4 cut(s) 536, 570, 2027, 3477
HindIII AAGCTT 3 cut(s) 3272, 3305, 3580
HpaI GTTAAC 1 cut(s) 570
HpaII CCGG 3 cut(s) 3634, 3915, 4020
HpyCH4IV ACGT 4 cut(s) 278, 805, 2023, 3663
HpyF10VI GCNNNNNNNGC 9 cut(s) 218, 413, 673, 2147, 2159, 2773, 3166, 3230, 3815
HpySE526I ACGT 4 cut(s) 278, 805, 2023, 3663
Hsp92I GRCGYC 1 cut(s) 2023
HspAI GCGC 8 cut(s) 212, 1762, 2416, 2431, 2512, 2527, 2560, 2987
KroI GCCGGC 1 cut(s) 3633
KroNI GCCGGC 1 cut(s) 3635
KspAI GTTAAC 1 cut(s) 570
MabI ACCWGGT 1 cut(s) 1401
MaeI CTAG 9 cut(s) 887, 968, 1734, 2019, 2030, 2103, 3153, 3417, 3771
MaeII ACGT 4 cut(s) 278, 805, 2023, 3663
MaeIII GTNAC 6 cut(s) 197, 226, 2672, 2694, 3795, 3841
MflI RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3140, 3364, 3563
MhlI GDGCHC 9 cut(s) 1046, 1165, 1390, 1904, 2320, 2977, 3046, 3645, 4039
MlsI TGGCCA 1 cut(s) 2349
MluNI TGGCCA 1 cut(s) 2349
MmeI TCCRAC 7 cut(s) 46, 70, 303, 3297, 3800, 4011, 4064
Mox20I TGGCCA 1 cut(s) 2349
Mph1103I ATGCAT 1 cut(s) 482
MroNI GCCGGC 1 cut(s) 3633
MroXI GAANNNNTTC 1 cut(s) 1987
MscI TGGCCA 1 cut(s) 2349
MslI CAYNNNNRTG 5 cut(s) 392, 1491, 1700, 2079, 3444
Msp20I TGGCCA 1 cut(s) 2349
MspA1I CMGCKG 2 cut(s) 1562, 3839
MspI CCGG 3 cut(s) 3634, 3915, 4020
MvnI CGCG 2 cut(s) 214, 2949
MwoI GCNNNNNNNGC 9 cut(s) 218, 413, 673, 2147, 2159, 2773, 3166, 3230, 3815
NaeI GCCGGC 1 cut(s) 3635
NcoI CCATGG 2 cut(s) 2074, 2760
NdeI CATATG 1 cut(s) 2311
NgoMIV GCCGGC 1 cut(s) 3633
NmeAIII GCCGAG 1 cut(s) 1078
NmuCI GTSAC 3 cut(s) 197, 226, 2694
NsbI TGCGCA 3 cut(s) 1763, 2432, 2528
NsiI ATGCAT 1 cut(s) 482
NspI RCATGY 1 cut(s) 2962
OliI CACNNNNGTG 3 cut(s) 392, 1700, 3444
PagI TCATGA 1 cut(s) 771
PasI CCCWGGG 1 cut(s) 2993
PciI ACATGT 1 cut(s) 2958
PcsI WCGNNNNNNNCGW 1 cut(s) 3895
PdiI GCCGGC 1 cut(s) 3635
PdmI GAANNNNTTC 1 cut(s) 1987
PflFI GACNNNGTC 1 cut(s) 2835
PflMI CCANNNNNTGG 2 cut(s) 1529, 3038
PfoI TCCNGGA 3 cut(s) 444, 1927, 3849
PmaCI CACGTG 1 cut(s) 806
PmlI CACGTG 1 cut(s) 806
Ppu21I YACGTR 2 cut(s) 279, 806
PpuMI RGGWCCY 1 cut(s) 3056
PscI ACATGT 1 cut(s) 2958
PshBI ATTAAT 1 cut(s) 3426
Psp124BI GAGCTC 2 cut(s) 1046, 3645
Psp5II RGGWCCY 1 cut(s) 3056
PspCI CACGTG 1 cut(s) 806
PspFI CCCAGC 2 cut(s) 1295, 2598
PspPI GGNCC 7 cut(s) 68, 325, 860, 1925, 3056, 3895, 4013
PspPPI RGGWCCY 1 cut(s) 3056
PstI CTGCAG 4 cut(s) 163, 2038, 3211, 3235
PstNI CAGNNNCTG 1 cut(s) 3230
PsuI RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3140, 3364, 3563
PsyI GACNNNGTC 1 cut(s) 2835
PvuII CAGCTG 1 cut(s) 3839
RsaI GTAC 9 cut(s) 297, 870, 1064, 1771, 2040, 2376, 2622, 2871, 3458
RsaNI GTAC 9 cut(s) 296, 869, 1063, 1770, 2039, 2375, 2621, 2870, 3457
RseI CAYNNNNRTG 5 cut(s) 392, 1491, 1700, 2079, 3444
SacI GAGCTC 2 cut(s) 1046, 3645
Sau96I GGNCC 7 cut(s) 68, 325, 860, 1925, 3056, 3895, 4013
ScaI AGTACT 2 cut(s) 1771, 2040
SduI GDGCHC 9 cut(s) 1046, 1165, 1390, 1904, 2320, 2977, 3046, 3645, 4039
SexAI ACCWGGT 1 cut(s) 1401
SfcI CTRYAG 9 cut(s) 159, 222, 2034, 2220, 2737, 3094, 3207, 3231, 3834
SinI GGWCC 5 cut(s) 68, 325, 860, 1925, 3056
SmiMI CAYNNNNRTG 5 cut(s) 392, 1491, 1700, 2079, 3444
SmlI CTYRAG 1 cut(s) 2254
SmoI CTYRAG 1 cut(s) 2254
SsiI CCGC 9 cut(s) 116, 214, 1562, 1671, 2160, 2389, 2575, 2947, 3976
SspMI CTAG 9 cut(s) 887, 968, 1734, 2019, 2030, 2103, 3153, 3417, 3771
SstI GAGCTC 2 cut(s) 1046, 3645
StyI CCWWGG 3 cut(s) 340, 2074, 2760
TaiI ACGT 4 cut(s) 281, 808, 2026, 3666
TaqI TCGA 7 cut(s) 656, 1390, 2043, 2830, 3430, 3673, 3889
TatI WGTACW 7 cut(s) 295, 1769, 2038, 2374, 2620, 2869, 3456
TauI GCSGC 2 cut(s) 119, 3979
TscAI CASTG 8 cut(s) 235, 262, 606, 995, 1330, 1954, 3142, 3299
TseFI GTSAC 3 cut(s) 197, 226, 2694
Tsp45I GTSAC 3 cut(s) 197, 226, 2694
TspGWI ACGGA 4 cut(s) 2158, 2260, 2829, 3061
TspRI CASTG 8 cut(s) 235, 262, 606, 995, 1330, 1954, 3142, 3299
Tth111I GACNNNGTC 1 cut(s) 2835
Van91I CCANNNNNTGG 2 cut(s) 1529, 3038
VneI GTGCAC 1 cut(s) 3042
VpaK11BI GGWCC 5 cut(s) 68, 325, 860, 1925, 3056
VspI ATTAAT 1 cut(s) 3426
XapI RAATTY 8 cut(s) 464, 1187, 1570, 2380, 2707, 2902, 3121, 3396
XbaI TCTAGA 1 cut(s) 3152
XceI RCATGY 1 cut(s) 2962
XcmI CCANNNNNNNNNTGG 1 cut(s) 1702
XmiI GTMKAC 3 cut(s) 2437, 2485, 2533
XmnI GAANNNNTTC 1 cut(s) 1987
XspI CTAG 9 cut(s) 887, 968, 1734, 2019, 2030, 2103, 3153, 3417, 3771
ZraI GACGTC 1 cut(s) 2024
ZrmI AGTACT 2 cut(s) 1771, 2040
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.