pycom12g11590

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002662
Physical Location & Seq
Reverse (-)
13870794 .. 13876694
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11590.1

Sequence Viewer

Length: 3951 bp
ATGGAATCCGCCAATCCTAATGCTAACATTATGCCGGGTGTTCTACAGCGGTTGATTCCTGTTGTTGGACCTGTGCTTCTGATTTCAGTTGGATATATTGACCCTGGAAAGTGGGCAGCAACTGCTGAAGCAGGTGCCTGTTTTGGCTCTGACCTTGCAGCATTGATGCTTATTTTCAATTTGGCAGCTATTTTATGTCACTATCTGTCAGCTCGGATTGGTGTAGTCACCGGAAGAGATCTTGCACAGATATGCAGTGAGGAGTATGACAAAGGCACTTGCATATCCTTAGGAATTCAAACTGAGGTTTCTGCGATCCTGTCAGACCTAACCATGGTCCTTGGCATTGCACATGGGCTTAATCTTCTGTTTGGTTGGGACCTGTTCACTTGTGTCTTTTTGACTGCCGTTAATGCTGTTCTATACCCTCTTTTTTCCACCTTCCTGGAAACTTGCAAAGCGAAGGTTGTATGCATATATATTACAGGATTTATACTCCTTTCCTTTGTTCTTGGAGTAATTATCAGTCAACCAGAAATGCCCCATTCCATGAATGGGATGCTAACAAAGCTGAGTGGGGAGAGTGCATTTGCGCTGATGAGCCTTCTTGGAGCCAGCATAATGCCTCACAGTCTGTATTTTCATTCTTCTATTGTGCAGCAATATCAGCAGCAGGCAACTGTTCCCAAGGATGCTTTGTGTCATAACCATTTAGTTGCCATCATATGCATCTTCAGTGGCATTTACATGGTGAATTATGTCCTTATGACCTCAGCAGAAAATTTATTCTACAGTACAGAGACAAGTTCAGGCCTTGTTCTTACGTTTCAGGATGCAATGTCACTAATGGAACAGGTCTTTTGCGGTCCAATAGTGCCAGCTTCCTTGTTACTGGTTCTCGTTCTGTCAAATCAAATTACAACATTAAGCTGGAGTCTGGGTGGGGAAGTAGTCCTGAATGAATTTTTGAAAGTGGACCTTCCTGGTTGGCTTCATTGTGCTACAATCAGAATTATTGCCATTGTTCCAGCCCTTTATTTTGTTTGGAGTTCGGGAGCTGAAGGAATGTATCAACTGCTTATATTCACACAAGTTTTGGCAGCTCTGCTACTGCCACCTTCTGTGATCCCTATTTTTCGAATTGCTGCTTCAAGACCAAGAATGGGCGTCCATAAAATTTCTCAATTTGTTGAATTCTTATCCCTGATTACGCTTATTGGGATGCTTGGATTGAAAGTTATATTTGTAGTAGAAATGATTTTTGGGAATAGTGAATGGGGTGGTAACTTGAGGTCGAACAGTGGTAGTAGTATGTCACTATTCCTCACTGCTTGTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACCCCATTAAAATCTGCGAGTGTCCGATTAGAGGCTCAGGTGTGGAACTGGGAACTGAAGGGTACACCAGATTCGTTGAAAAATGAAGTGGAGAGTAACATAGCTGAACCTCAGTATCAGAGAGAGGCAAGTGTCCAGAACCATGAACCATCACCATCATTTGGGTTGGATGCTGATTCAGAAGTTGCAAATTTTGATCTTGATCTGCCTGACACTATCGTGGAGGCTGATCAGGAACTTCACCAAACAACTGTAGGGGAGAATAGTAATTCTCATATTACCTTTCCTACCTCCCCTAAAAGCTGCATGGAGGGATCCACATCTGCAGTGGAGTCAGTTCCAGTCGCAACTGTCGTTAATGATGTTTCTGATGTCACATTGGAGGACACCAGTGCACTGAAGGTCAAGTCAACAGAAATTGAGAAGACAGTTGAAGTTGAAGGAGTTGAAGGAGTTGAAGGAGACTTACCAACCGAAAAAGATGACGATGAGGGAGATACCTGGGAGCCTGAAGAATTATCAAAAGGGGTTTCTGGGAGCACTGCGCCATTGGCCTCCGAGGGGTCAGGATCATTTAGGACTCTAAGTGGAAAAGGTGATGATTTGGGGAGCAGTACAGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTTCTGCAAGACGTCAACTAGCTACTATGCTTGATGAATTTTGGGGACAAATGTATGATTTCCATGGGAATGTAATTCAAGACGCAAAGGCTAAGAAGCTGGACCTTTTGTTGGGATCAGATTCAAAGGCAGTGTCGTCCTCGCCGAAAGTTGATACTGGTGCAAAGGAATCTTTGGGATACTTTCCATCTGTAGGAGGCAGAGGATCTGATTCTCTTATCAACTCAAGTTTATATGACTCTGCAAAGCAGCATATGGTTCAAAGCAGTTTAGAGTCATATGCGGTCCAAAGGGGATCTTCCTCATTGTTGCCCAGCCACATGCAGTTGTTAGATGCGTATGTGCAAAGTTCAAGCCGTAGCATTATTGACTCTGATGAGAGGCGCTATCCAAGTGTGCGCAGTATCCCAACTTCTGAGAGTGGTGGGAAGCGCTATCCAAGTATGCGCAGTATCCCAACTTCTGAGAGTTGGGATTACCAGCCAGCTACAGTACATGGTTATCAGATTGCATCGTATCTCAATCGATTGTCAAAGGAAAAAAATTCTGATAACTTGAACCCTCAAATGGAGCCATCAATCCTAAAGTCCGGTTCTTCGTTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATAGGTTAGGATCTGGTCAGGTCTCCAATTTTCAGAACTTTACAGTATCTAGAAATAGTCCAATGCAATCTGGAAGACCGTATTATGATCCATGCTCTTCTGGAATTGCTGAGAATGTGGTAAGTCCAGCAAATGCAAAGAAATACCATAGTTTACCAGACATTCACCGGGAGCTTTACATGCCTGATAAAAATGCTAACTGGGAAAGTTCTCTCGGGCATGGATCATCTCCTGGGATGGCTGGTTATGAATCATCCTTGTATTCAAATTCTGGGGCACGAACAAGAGCTCCTTTGGCTTTTGATGAACTCTCTCCAAAAGTCTACAATTCTCTGTCATCACAACAGAGTTCTACTTTTAACACTGGGTCACTCTGGTCTAGACAGCCTTTTGAGCAGTTCGGTGTGGCTGATACTAGTCGTACTATTGGTAGTGGAATTGGTAATAGGGTAGGTTCTGTAAATCAAGAATCTACTTTGGTTGCATATTCAGAGGCCAAGCTTCTTCAGGCTTTCAGGCATTGCATTGTGAAGCTTTTGAAATTGGAAGGGTCGGACTGGTTGTTTACACAGAATGATGGGGTTGATGAGGATCTAATTGATTGCGTGGCTGCAAAGGAGAAATTTCTTTATGAAGCTGAAACTAGAGAGATAAATCGAACAGGTCAAGTGGGCGAGCCTCAGTACCATTCTTCAGATACGGAGTCTGCTTCAGCATTGAAGAATAATGATTTGAGTTATATTATGATTTCCTCAGTTCCACATTGTGGGGATGGTTGTATATGGAAGGCAGATTTGGTAGTAAGCTTTGGGGTTTGGTGTATCCATCGCATTCTTGATTTGTCACTTATGGAAAGCCGGCCAGAGCTATGGGGGAAATATACCTACGTTCTCAACCGACTTCAGGGGATTATTGATTCGGCATTTTCAAAGCCTCGTTCTCCAATGTCACCATGCATCTGCCTTCAAGTTCCCGCGGCACACCAGCTGCAATCGAGTCCATCATTTTCTAATGGAATACCTCCTCCTGCAAAACCAGCCAGGGGAAAATGCACAACGGCAGTAACGCTTCTAGACATGATCAAGGATGTGGAAATTGCAATCAATTCTCGAAAGGGCCGAACAGGGACAGCAGCTGGTGATGTAGCTTTCCCAATGGGAAAAGAAAATATGGCATCTGTCCTTAAACGCTACAAGCGTAGATTATCCAACAAAACTGTTGGCACTCATGAGGGTCCTGGTTCGGGCAAGTCTCTGACATCTGCTCCTTATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1317

Amino Acids

142.47

Weight (kDa)

5.35

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 394 4.2e-79 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 122
AatII GACGTC 1 cut(s) 2032
Acc16I TGCGCA 2 cut(s) 2417, 2465
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 1 cut(s) 134
AccB7I CCANNNNNTGG 2 cut(s) 1529, 3440
AccI GTMKAC 1 cut(s) 2997
AccII CGCG 1 cut(s) 3650
AciI CCGC 6 cut(s) 9, 49, 864, 2300, 3648, 3650
AcoI YGGCCR 1 cut(s) 3533
AcyI GRCGYC 2 cut(s) 1167, 2029
AdeI CACNNNGTG 1 cut(s) 3440
AfaI GTAC 6 cut(s) 796, 1432, 1984, 2511, 3097, 3359
AfeI AGCGCT 1 cut(s) 2450
AhlI ACTAGT 1 cut(s) 3089
AjnI CCWGG 7 cut(s) 103, 444, 982, 1868, 2905, 3713, 3910
AjuI GAANNNNNNNTTGG 4 cut(s) 1245, 1277, 3452, 3484
AleI CACNNNNGTG 1 cut(s) 1586
AloI GAACNNNNNNTCC 2 cut(s) 2947, 2979
Alw21I GWGCWC 3 cut(s) 1765, 1910, 2965
Alw26I GTCTC 4 cut(s) 794, 1824, 2701, 3930
Alw44I GTGCAC 1 cut(s) 1761
AlwNI CAGNNNCTG 2 cut(s) 122, 3809
Ama87I CYCGRG 1 cut(s) 2888
Aor51HI AGCGCT 1 cut(s) 2450
AoxI GGCC 5 cut(s) 811, 1920, 3168, 3533, 3790
ApaLI GTGCAC 1 cut(s) 1761
ArsI GACNNNNNNTTYG 2 cut(s) 2135, 2167
Asp700I GAANNNNTTC 1 cut(s) 1993
AspLEI GCGC 6 cut(s) 595, 1915, 2403, 2418, 2451, 2466
AspS9I GGNCC 9 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3790, 3908
AsuC2I CCSGG 2 cut(s) 36, 2843
AsuHPI GGTGA 8 cut(s) 220, 763, 1512, 1601, 1976, 2831, 3615, 3824
AsuII TTCGAA 1 cut(s) 1138
AvaI CYCGRG 1 cut(s) 2888
AvaII GGWCC 8 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3908
AxyI CCTNAGG 1 cut(s) 289
BaeGI GKGCMC 2 cut(s) 1765, 2953
BaeI ACNNNNGTAYC 2 cut(s) 1466, 1499
BamHI GGATCC 1 cut(s) 1682
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 2965
BarI GAAGNNNNNNTAC 6 cut(s) 1818, 1850, 2413, 2445, 2461, 2493
BbsI GAAGAC 2 cut(s) 1799, 2755
Bbv12I GWGCWC 3 cut(s) 1765, 1910, 2965
BbvCI CCTCAGC 1 cut(s) 772
BceAI ACGGC 3 cut(s) 392, 2358, 3747
BciT130I CCWGG 7 cut(s) 105, 446, 984, 1870, 2907, 3715, 3912
BciVI GTATCC 4 cut(s) 2189, 2432, 2480, 3506
BclI TGATCA 2 cut(s) 1597, 3753
BcnI CCSGG 2 cut(s) 36, 2843
BcoDI GTCTC 4 cut(s) 794, 1824, 2701, 3930
BcuI ACTAGT 1 cut(s) 3089
BfaI CTAG 6 cut(s) 2036, 2724, 3054, 3090, 3318, 3746
BfmI CTRYAG 7 cut(s) 44, 790, 1620, 1692, 2208, 2505, 2632
BfoI RGCGCY 2 cut(s) 2404, 2452
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 4 cut(s) 2189, 2432, 2480, 3506
BglII AGATCT 1 cut(s) 238
Bme1390I CCNGG 9 cut(s) 36, 105, 446, 984, 1870, 2843, 2907, 3715, 3912
Bme18I GGWCC 8 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3908
BmeT110I CYCGRG 1 cut(s) 2888
BmgT120I GGNCC 9 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3790, 3908
BmiI GGNNCC 7 cut(s) 136, 380, 613, 1684, 1875, 2589, 3909
BmrFI CCNGG 9 cut(s) 36, 105, 446, 984, 1870, 2843, 2907, 3715, 3912
BmrI ACTGGG 3 cut(s) 1426, 2884, 3048
BmuI ACTGGG 3 cut(s) 1426, 2884, 3048
BpiI GAAGAC 2 cut(s) 1799, 2755
BpmI CTGGAG 1 cut(s) 952
Bpu10I CCTNAGC 2 cut(s) 772, 1404
Bpu14I TTCGAA 1 cut(s) 1138
BpuEI CTTGAG 2 cut(s) 1309, 2227
BpuMI CCSGG 2 cut(s) 36, 2843
Bsa29I ATCGAT 1 cut(s) 2542
BsaBI GATNNNNATC 3 cut(s) 1569, 1687, 3264
BsaHI GRCGYC 2 cut(s) 1167, 2029
BsaI GGTCTC 1 cut(s) 2701
BsaWI WCCGGW 2 cut(s) 230, 2606
BsaXI ACNNNNNCTCC 5 cut(s) 925, 955, 2947, 2977, 3922
Bse118I RCCGGY 1 cut(s) 3531
Bse1I ACTGG 8 cut(s) 897, 1421, 1709, 1758, 2179, 2879, 3043, 3236
Bse21I CCTNAGG 1 cut(s) 289
Bse3DI GCAATG 3 cut(s) 345, 843, 3193
Bse8I GATNNNNATC 3 cut(s) 1569, 1687, 3264
BseBI CCWGG 7 cut(s) 105, 446, 984, 1870, 2907, 3715, 3912
BseCI ATCGAT 1 cut(s) 2542
BseGI GGATG 9 cut(s) 564, 697, 838, 1227, 1543, 2916, 2927, 3451, 3766
BseJI GATNNNNATC 3 cut(s) 1569, 1687, 3264
BseMI GCAATG 3 cut(s) 345, 843, 3193
BseNI ACTGG 8 cut(s) 897, 1421, 1709, 1758, 2179, 2879, 3043, 3236
BseRI GAGGAG 2 cut(s) 275, 3687
BseSI GKGCMC 2 cut(s) 1765, 2953
BseYI CCCAGC 1 cut(s) 2330
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 1 cut(s) 3650
BshFI GGCC 5 cut(s) 813, 1922, 3170, 3535, 3792
BshNI GGYRCC 1 cut(s) 134
BshVI ATCGAT 1 cut(s) 2542
BsiHKAI GWGCWC 3 cut(s) 1765, 1910, 2965
BsiHKCI CYCGRG 1 cut(s) 2888
BsiSI CCGG 5 cut(s) 35, 231, 2607, 2842, 3532
BslFI GGGAC 3 cut(s) 392, 2076, 3814
BsmAI GTCTC 4 cut(s) 794, 1824, 2701, 3930
BsmFI GGGAC 3 cut(s) 392, 2076, 3814
BsmI GAATGC 1 cut(s) 3504
BsnI GGCC 5 cut(s) 813, 1922, 3170, 3535, 3792
Bso31I GGTCTC 1 cut(s) 2701
BsoBI CYCGRG 1 cut(s) 2888
Bsp119I TTCGAA 1 cut(s) 1138
Bsp1286I GDGCHC 4 cut(s) 1765, 1910, 2953, 2965
Bsp19I CCATGG 3 cut(s) 333, 2080, 2655
BspACI CCGC 6 cut(s) 9, 49, 864, 2300, 3648, 3650
BspANI GGCC 5 cut(s) 813, 1922, 3170, 3535, 3792
BspDI ATCGAT 1 cut(s) 2542
BspFNI CGCG 1 cut(s) 3650
BspHI TCATGA 1 cut(s) 3901
BspLI GGNNCC 7 cut(s) 136, 380, 613, 1684, 1875, 2589, 3909
BspMAI CTGCAG 1 cut(s) 1696
BspMI ACCTGC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 2776
BspT104I TTCGAA 1 cut(s) 1138
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 2701
BsrDI GCAATG 3 cut(s) 345, 843, 3193
BsrFI RCCGGY 1 cut(s) 3531
BsrI ACTGG 8 cut(s) 897, 1421, 1709, 1758, 2179, 2879, 3043, 3236
BssAI RCCGGY 1 cut(s) 3531
BssNI GRCGYC 2 cut(s) 1167, 2029
BssT1I CCWWGG 5 cut(s) 333, 340, 687, 2080, 2655
Bst2UI CCWGG 7 cut(s) 105, 446, 984, 1870, 2907, 3715, 3912
Bst6I CTCTTC 2 cut(s) 229, 2776
BstACI GRCGYC 2 cut(s) 1167, 2029
BstAPI GCANNNNNTGC 2 cut(s) 122, 2668
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 7 cut(s) 616, 675, 879, 1362, 2502, 3350, 3533
BstDSI CCRYGG 4 cut(s) 333, 2080, 2655, 3648
BstF5I GGATG 9 cut(s) 564, 697, 838, 1227, 1543, 2916, 2927, 3451, 3766
BstFNI CGCG 1 cut(s) 3650
BstH2I RGCGCY 2 cut(s) 2404, 2452
BstHHI GCGC 6 cut(s) 595, 1915, 2403, 2418, 2451, 2466
BstMAI GTCTC 4 cut(s) 794, 1824, 2701, 3930
BstNI CCWGG 7 cut(s) 105, 446, 984, 1870, 2907, 3715, 3912
BstNSI RCATGY 2 cut(s) 2341, 2857
BstSCI CCNGG 9 cut(s) 34, 103, 444, 982, 1868, 2841, 2905, 3713, 3910
BstSFI CTRYAG 7 cut(s) 44, 790, 1620, 1692, 2208, 2505, 2632
BstSLI GKGCMC 2 cut(s) 1765, 2953
BstUI CGCG 1 cut(s) 3650
BstV2I GAAGAC 2 cut(s) 1799, 2755
BstX2I RGATCY 6 cut(s) 238, 1682, 2222, 2312, 2684, 3265
BstXI CCANNNNNNTGG 2 cut(s) 445, 3543
BstYI RGATCY 6 cut(s) 238, 1682, 2222, 2312, 2684, 3265
Bsu15I ATCGAT 1 cut(s) 2542
Bsu36I CCTNAGG 1 cut(s) 289
BsuI GTATCC 4 cut(s) 2189, 2432, 2480, 3506
BsuRI GGCC 5 cut(s) 813, 1922, 3170, 3535, 3792
BsuTUI ATCGAT 1 cut(s) 2542
BtgI CCRYGG 4 cut(s) 333, 2080, 2655, 3648
BtgZI GCGATG 1 cut(s) 3485
BtsCI GGATG 9 cut(s) 564, 697, 838, 1227, 1543, 2916, 2927, 3451, 3766
BtsI GCAGTG 5 cut(s) 262, 1326, 1701, 1908, 2154
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 7 cut(s) 616, 675, 879, 1362, 2502, 3350, 3533
CaiI CAGNNNCTG 2 cut(s) 122, 3809
CciI TCATGA 1 cut(s) 3901
CfoI GCGC 6 cut(s) 595, 1915, 2403, 2418, 2451, 2466
Cfr10I RCCGGY 1 cut(s) 3531
Cfr13I GGNCC 9 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3790, 3908
Cfr42I CCGCGG 1 cut(s) 3651
ClaI ATCGAT 1 cut(s) 2542
CseI GACGC 2 cut(s) 1156, 2108
Csp6I GTAC 6 cut(s) 795, 1431, 1983, 2510, 3096, 3358
CviQI GTAC 6 cut(s) 795, 1431, 1983, 2510, 3096, 3358
DraIII CACNNNGTG 1 cut(s) 3440
EaeI YGGCCR 1 cut(s) 3533
Eam1104I CTCTTC 2 cut(s) 229, 2776
EarI CTCTTC 2 cut(s) 229, 2776
Ecl136II GAGCTC 1 cut(s) 2963
Eco130I CCWWGG 5 cut(s) 333, 340, 687, 2080, 2655
Eco147I AGGCCT 1 cut(s) 813
Eco24I GRGCYC 1 cut(s) 2965
Eco31I GGTCTC 1 cut(s) 2701
Eco47I GGWCC 8 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3908
Eco47III AGCGCT 1 cut(s) 2450
Eco53kI GAGCTC 1 cut(s) 2963
Eco81I CCTNAGG 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 2888
EcoICRI GAGCTC 1 cut(s) 2963
EcoO109I RGGNCCY 2 cut(s) 379, 3908
EcoRI GAATTC 2 cut(s) 294, 1193
EcoRII CCWGG 7 cut(s) 103, 444, 982, 1868, 2905, 3713, 3910
EcoT14I CCWWGG 5 cut(s) 333, 340, 687, 2080, 2655
EcoT22I ATGCAT 3 cut(s) 476, 731, 3632
EcoT38I GRGCYC 1 cut(s) 2965
ErhI CCWWGG 5 cut(s) 333, 340, 687, 2080, 2655
FalI AAGNNNNNCTT 6 cut(s) 963, 995, 2299, 2331, 2950, 2982
FaqI GGGAC 3 cut(s) 392, 2076, 3814
FauI CCCGC 1 cut(s) 3655
FauNDI CATATG 3 cut(s) 725, 2271, 2296
FbaI TGATCA 2 cut(s) 1597, 3753
FblI GTMKAC 1 cut(s) 2997
FokI GGATG 9 cut(s) 571, 704, 845, 1234, 1550, 2914, 2923, 3458, 3773
FriOI GRGCYC 1 cut(s) 2965
FspBI CTAG 6 cut(s) 2036, 2724, 3054, 3090, 3318, 3746
FspI TGCGCA 2 cut(s) 2417, 2465
GlaI GCGC 6 cut(s) 594, 1914, 2402, 2417, 2450, 2465
GsaI CCCAGC 1 cut(s) 2334
GsuI CTGGAG 1 cut(s) 952
HaeII RGCGCY 2 cut(s) 2404, 2452
HaeIII GGCC 5 cut(s) 813, 1922, 3170, 3535, 3792
HapII CCGG 5 cut(s) 35, 231, 2607, 2842, 3532
HgaI GACGC 2 cut(s) 1156, 2108
HhaI GCGC 6 cut(s) 595, 1915, 2403, 2418, 2451, 2466
Hin1I GRCGYC 2 cut(s) 1167, 2029
Hin6I GCGC 6 cut(s) 593, 1913, 2401, 2416, 2449, 2464
HinP1I GCGC 6 cut(s) 593, 1913, 2401, 2416, 2449, 2464
HincII GTYRAC 3 cut(s) 530, 1779, 2033
HindII GTYRAC 3 cut(s) 530, 1779, 2033
HindIII AAGCTT 3 cut(s) 3173, 3206, 3478
HpaII CCGG 5 cut(s) 35, 231, 2607, 2842, 3532
HphI GGTGA 8 cut(s) 220, 763, 1512, 1601, 1976, 2831, 3615, 3824
HpyCH4IV ACGT 3 cut(s) 824, 2029, 3561
HpySE526I ACGT 3 cut(s) 824, 2029, 3561
Hsp92I GRCGYC 2 cut(s) 1167, 2029
HspAI GCGC 6 cut(s) 593, 1913, 2401, 2416, 2449, 2464
KroI GCCGGC 1 cut(s) 3531
KroNI GCCGGC 1 cut(s) 3533
Ksp22I TGATCA 2 cut(s) 1597, 3753
KspI CCGCGG 1 cut(s) 3651
LguI GCTCTTC 1 cut(s) 2776
LmnI GCTCC 9 cut(s) 611, 1055, 1873, 1905, 1977, 2587, 2845, 2968, 3943
MaeI CTAG 6 cut(s) 2036, 2724, 3054, 3090, 3318, 3746
MaeII ACGT 3 cut(s) 824, 2029, 3561
MflI RGATCY 6 cut(s) 238, 1682, 2222, 2312, 2684, 3265
MhlI GDGCHC 4 cut(s) 1765, 1910, 2953, 2965
MlyI GAGTC 8 cut(s) 943, 1709, 1942, 2249, 2300, 2381, 3386, 3679
MmeI TCCRAC 5 cut(s) 46, 70, 1515, 3207, 3906
Mph1103I ATGCAT 3 cut(s) 476, 731, 3632
MroNI GCCGGC 1 cut(s) 3531
MroXI GAANNNNTTC 1 cut(s) 1993
MseI TTAA 7 cut(s) 360, 411, 926, 1376, 1725, 3033, 3858
MslI CAYNNNNRTG 4 cut(s) 250, 746, 1586, 2085
MspA1I CMGCKG 4 cut(s) 49, 3650, 3661, 3809
MspI CCGG 5 cut(s) 35, 231, 2607, 2842, 3532
MspR9I CCNGG 9 cut(s) 36, 105, 446, 984, 1870, 2843, 2907, 3715, 3912
Mva1269I GAATGC 1 cut(s) 3504
MvaI CCWGG 7 cut(s) 105, 446, 984, 1870, 2907, 3715, 3912
MvnI CGCG 1 cut(s) 3650
NaeI GCCGGC 1 cut(s) 3533
NciI CCSGG 2 cut(s) 36, 2843
NcoI CCATGG 3 cut(s) 333, 2080, 2655
NdeI CATATG 3 cut(s) 725, 2271, 2296
NgoMIV GCCGGC 1 cut(s) 3531
NlaIV GGNNCC 7 cut(s) 136, 380, 613, 1684, 1875, 2589, 3909
NmuCI GTSAC 8 cut(s) 197, 226, 840, 1314, 1741, 3042, 3516, 3621
NsbI TGCGCA 2 cut(s) 2417, 2465
NsiI ATGCAT 3 cut(s) 476, 731, 3632
NspI RCATGY 2 cut(s) 2341, 2857
NspV TTCGAA 1 cut(s) 1138
OliI CACNNNNGTG 1 cut(s) 1586
PagI TCATGA 1 cut(s) 3901
PaqCI CACCTGC 1 cut(s) 122
PceI AGGCCT 1 cut(s) 813
PciSI GCTCTTC 1 cut(s) 2776
PcsI WCGNNNNNNNCGW 4 cut(s) 1719, 2159, 3790, 3868
PctI GAATGC 1 cut(s) 3504
PdiI GCCGGC 1 cut(s) 3533
PdmI GAANNNNTTC 1 cut(s) 1993
PflMI CCANNNNNTGG 2 cut(s) 1529, 3440
PfoI TCCNGGA 1 cut(s) 444
PleI GAGTC 8 cut(s) 942, 1708, 1942, 2249, 2299, 2381, 3385, 3678
PpsI GAGTC 8 cut(s) 942, 1708, 1942, 2249, 2299, 2381, 3385, 3678
PpuMI RGGWCCY 2 cut(s) 379, 3908
Psp124BI GAGCTC 1 cut(s) 2965
Psp5II RGGWCCY 2 cut(s) 379, 3908
Psp6I CCWGG 7 cut(s) 103, 444, 982, 1868, 2905, 3713, 3910
PspFI CCCAGC 1 cut(s) 2330
PspGI CCWGG 7 cut(s) 103, 444, 982, 1868, 2905, 3713, 3910
PspN4I GGNNCC 7 cut(s) 136, 380, 613, 1684, 1875, 2589, 3909
PspPI GGNCC 9 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3790, 3908
PspPPI RGGWCCY 2 cut(s) 379, 3908
PstI CTGCAG 1 cut(s) 1696
PstNI CAGNNNCTG 2 cut(s) 122, 3809
PsuI RGATCY 6 cut(s) 238, 1682, 2222, 2312, 2684, 3265
PvuII CAGCTG 2 cut(s) 3661, 3809
RsaI GTAC 6 cut(s) 796, 1432, 1984, 2511, 3097, 3359
RsaNI GTAC 6 cut(s) 795, 1431, 1983, 2510, 3096, 3358
RseI CAYNNNNRTG 4 cut(s) 250, 746, 1586, 2085
SacI GAGCTC 1 cut(s) 2965
SacII CCGCGG 1 cut(s) 3651
SapI GCTCTTC 1 cut(s) 2776
SaqAI TTAA 7 cut(s) 360, 411, 926, 1376, 1725, 3033, 3858
Sau96I GGNCC 9 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3790, 3908
SchI GAGTC 8 cut(s) 943, 1709, 1942, 2249, 2300, 2381, 3386, 3679
ScrFI CCNGG 9 cut(s) 36, 105, 446, 984, 1870, 2843, 2907, 3715, 3912
SduI GDGCHC 4 cut(s) 1765, 1910, 2953, 2965
SfcI CTRYAG 7 cut(s) 44, 790, 1620, 1692, 2208, 2505, 2632
Sfr303I CCGCGG 1 cut(s) 3651
SfuI TTCGAA 1 cut(s) 1138
SgrBI CCGCGG 1 cut(s) 3651
SinI GGWCC 8 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3908
SmiMI CAYNNNNRTG 4 cut(s) 250, 746, 1586, 2085
SmlI CTYRAG 2 cut(s) 1288, 2242
SmoI CTYRAG 2 cut(s) 1288, 2242
SpeI ACTAGT 1 cut(s) 3089
SseBI AGGCCT 1 cut(s) 813
SsiI CCGC 6 cut(s) 9, 49, 864, 2300, 3648, 3650
SspMI CTAG 6 cut(s) 2036, 2724, 3054, 3090, 3318, 3746
SstI GAGCTC 1 cut(s) 2965
StuI AGGCCT 1 cut(s) 813
StyD4I CCNGG 9 cut(s) 34, 103, 444, 982, 1868, 2841, 2905, 3713, 3910
StyI CCWWGG 5 cut(s) 333, 340, 687, 2080, 2655
TaiI ACGT 3 cut(s) 827, 2032, 3564
TaqI TCGA 6 cut(s) 1138, 1295, 2542, 3331, 3668, 3784
TatI WGTACW 3 cut(s) 794, 1982, 2509
TauI GCSGC 1 cut(s) 3653
Tru1I TTAA 7 cut(s) 360, 411, 926, 1376, 1725, 3033, 3858
Tru9I TTAA 7 cut(s) 360, 411, 926, 1376, 1725, 3033, 3858
TseFI GTSAC 8 cut(s) 197, 226, 840, 1314, 1741, 3042, 3516, 3621
Tsp45I GTSAC 8 cut(s) 197, 226, 840, 1314, 1741, 3042, 3516, 3621
TspGWI ACGGA 1 cut(s) 3389
Van91I CCANNNNNTGG 2 cut(s) 1529, 3440
VneI GTGCAC 1 cut(s) 1761
VpaK11BI GGWCC 8 cut(s) 68, 337, 379, 866, 976, 2119, 2302, 3908
XbaI TCTAGA 3 cut(s) 2723, 3053, 3745
XceI RCATGY 2 cut(s) 2341, 2857
XcmI CCANNNNNNNNNTGG 2 cut(s) 551, 1693
XmiI GTMKAC 1 cut(s) 2997
XmnI GAANNNNTTC 1 cut(s) 1993
XspI CTAG 6 cut(s) 2036, 2724, 3054, 3090, 3318, 3746
ZraI GACGTC 1 cut(s) 2030
Zsp2I ATGCAT 3 cut(s) 476, 731, 3632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.