Rh3DG197700

Ethylene-insensitive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002662
Physical Location & Seq
Reverse (-)
17770255 .. 17776360
6106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG197700.1

Sequence Viewer

Length: 4062 bp
ATGGAATCTGCTAGCTCTAACGCTAACAATATGCCAGGCGTTGTCCATCAGTTGCTTCCTGTTCTTGGACCCATGCTTTTGATTGCAGTTGGATATCTTGACCCTGGAAAGTGGGCGGCAACTGTTGAAGCAGGTTCCCATTATGGAACTGATCTGGCTGCAGTGATGCTTATATTCAATTTGGCTGCTATTTTATGTCACTATCTGTCAGCACGGATTGCTGTAGTCACTGGAAGAGATCTTGCTCAGGTTTGCAGTGAGGAGTATGACAAGGCTACGTGCATATTCTTAGGAGTACAAACAGAGATGTCGGTGATTGTGTTGGACCTTACCATGATCCTCGGTATCGCACATGCACTTAATCTTCTGTTTGGGTGGGACTTGTTCACATGTGTGTTTTTGACTGCTGCTAATGCTGTTTTATACCCTCTTTTTTCCACCCTCCTGGAGACTTGCAAGGCGAAATTCCTTTGCGTATGCATATACATTGCAGGATTTCTACTGCTTTCCTTTGTTCTTGGAGTATTTATCAGTCAACCACAAGTGCCACTTTCCATGACTGGGATGTTAACAAAACTGAGTGGGGAAAGTGCTTTTTCACTGATGAGTCTTCTTGGAGCAAGTATAATGCCCCACAGTTTTTATCTTCATTCTTCTATTGTGCAGCAGCATCAGCAACAACCAACTGTTTCTAAGGATGCCTTGTGTCAGAACCATTTTGTTGCCATCTTCTGCACCTTTAGTGGTATTTATCTGGTGAATTATGCCCTCATGACCTTAGCAGCAAATGTATTCTACACTTCACGTGGTTTGCTCACATTTCAGGATGCAATGTCCCTAATGGAACAGGTCTTTTGGGGTCCAATAGTACCTGTTGCCTTCTTGCTAGTTCTCTTTTTATCAAATCAAATCACAACATTAAGCTGGAGTCTGGGGGGACAAGTAGTCTTGAATGATTTTTTAAAATTAGACCTTCCTGGTTGGCTTCACTGTGCTACAATCAGAATCATTGCCATTGTTCCTGCTCTGTATTTTGTTTGGAGCTCAGGAGCTGAGGGGATGTACCAACTGCTTATATCTACACAGGTATTGGCAGCCTTGCTACTGCCGTCTTCTGTGATCCCTCTTTTTCGTGTTGCTGCTTCAAGACAAATAATGGGAGCCCATAAGATCTCTCAGTTTGTTGAATTCTTGGCCCTCATTACACTTATTGGGATGCTTGGATTAAAACTTGTCTTTGTAGTGGAAATGATTTTTGGGAATAGTGATTGGGTGGATAATTTGAGATGGGATGCTGGGAGTAGTATGTCTGCACTTCTCATCACTGCTTCTGCATCATTTTGTTTGATGATTTGGCTGGCAGCTACACCGTTAAAATCTGCAAGTGCTCGATTAGAGAACCAGGTGTGGAACTGGGATATGCCAAAGGGTGTATCTGAGCCATTTAGAAATAAGGAGGAGATTGATATAGCTGAACCTAATTATCATAGAGATGCAAGTGTTCAGAAGCATGAACCATCACCATCTTCTGGGAAGGCTTTGGATAGAGACTCGGATACAGCGGTTGCAAATTTTGATTTTGTTCTGCCCGAAACTCTCTTGGAGCCTGATCAGGAGCTTCAATCAACTATCTCAGAGGAAAATAGTTCTCTCAATACCTTTCCTCGCTCTGCCAAATGCAGTAAGGAGGAACCCACATCTGTGGTGGAGTCAATTCGCCTCCCAACTGTGGCTAGTGAGGTTTCTGATGTTACATCACTGGGCACCAGTACTGTGAAAGTTGGATCAACAGAGCCAGTTGAGAAGATTCTTGGAGTTGAAGGAAACTTACCAACTGAAAAAGATGATGATGAGGGAGATACCTGGGAACCTGAAGATTCCTTGAAAGAGGTTTCTGGGGGCACCACTTCATTGACATCTGAAGGTCCAGGATCATTCAGGAGTCTCAGTGGAAAAGGTGATGAAGGGGGGAGTGGTGCTGGAAGCCTTTCAAGATTAGCAGGGTTGGGGCGTGCTGCTAGACGTCAACTAGCGGCAGTACTCGATGAGTTTTGGGGACAACTGTATGATTTCCATGGTAATGTAATTCAAGAAGCAAAGGCTAGGAAACTGGATCTGTTGTTGGGGTCAGATTCAAAGGCTTCTTCTGCTGCTTCCTCCGTTTCCTCCATGCTGAAAGATGATACCACTGCAAAGGAAGTTTCTGGATACTTTCCATCTGTAGGAGGCAGAGGATCTGATCCTTTAATCAACTCAAGTTTATATGACTCCGTAAATCAGCCAAGGCTGCAAAACAGTTTAGAGTCATCATATGGTGCTCAAAGGGGATCTTCCTCATTATGGTCTGGCCACATACAGTTGCTGGATGCGTATGTACAAAATTCAACCCGCAGTATTATTGACTCGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCATCTTCAGACTTGGGGGAGAGGCGCTATTCAAGTGTGCGCAGTATACCATCTTCTGACTTGGGTGAGAGGCGCTATTCAAGTGTGCGCAGTATACCAACTTCTGACTCGGGTGAGAGGCGCTATTCAAGTGTCCGCAGCATACCATCTGCTGAGAGCTGGGATTACCAGCCAGCCACAGTACACGGTTATCAGATTTCATCGTATCTTAATCGTAATGACAGAAGTTCTGGTAACTTGAATGGTCAAATGGAATCACCAGCCCTAAATTCTGCTTCATTGGGTGCTGGAAACTACAGAGATTCACTTGCATTTACCATGGGGCAGAAGTTGCAAAATGGGTTAGGCTCTGCACAGGCCTCCAGTTTTCAGAACCTTACGGTATCTCGACATAGTCCGTTGCAATCTGAAAGACCATATTATGATGTACACTCTTCTGGACTTTCTGAGAATGTGGTAAATTCAGTCAATGCAAAGAAATACCACAGTTTACCTGACATTCACCGCGATCTCTACATGTCTAATAAAAGTGCTCAACGGGATGCTCCCCCTGGGTTTGGGAAAACTAATTATGAATCATCCTTGTATCCAAAATCTGGTGCACGGACAGGAGGTCCTTTGGCATTTGATGAACTCTCTCCATCAAAAGTCTATAGAGATGTTCTATCATCCCAACAGAATTCTAATTTTGGCACTGGATCTCTCTGGTCTAGACAGCCTTTTGAGCAATTTGGTGTAGCTGATAATAATCGTTCTATTGGGACTGCAGTTGGTAGTAGAGCAGGTTCTGCAGGTCAGGAAGCTATGTCAGTTGCAGATTCAGAGGCGAAGCTTCTTCAGTCTTTTAGACACTGCATTGTGAAGCTTTTGAAGTTGGAAGGGTCTGACTGGTTGTTTAGACAGAATGATGGAGTGGATGAGGATCTAATAGATCGTGTGGCTGCCAGGGAGAAAATTCTTTATGAAGCTGAAACTAGAGAGATTAATCGAACAGTTCACATGGGTGAATCTCAGTACACTTCTGACAGGAAGTCAACTTCTGCAATAAAAATGAGTGATGCAAATCTCACCCATCTCATGGTTTCCTCAGTTCCTAATTGTGGGGAAGGTTGTATCTGGAGATCTGATCTAATAATAAGCTTTGGGGTCTGGTGTATCCATCGGATTCTTGATTTGTCCCTTATGGAAAGCCGGCCAGAGCTCTGGGGGAAATATACTTACGTTCTCAATCGACTTCAGGGGATTATTGATTCTGCGTTTTCGAAGCCTCGTTCTCCAATGTCTCCATGCTTCTGCCTTCAAATTGCAGCAGCACAGCAGCAGAAGTCTAGTCCAACATTTTCGAATGGAATGTTACCCCCTGCTGCAAAACCAGCCAGGGGAAAATGCACTACAGCTGCAACGCTGCTGGACACAATCAAGGATGTGGAGACTGCAATCTCTTGTCGAAAGGGCCGAACGGGGACGGCAGCCGGTGATGTGGCTTTCCCGAAGGGAAAAGAAAATCTGGCATCTGTCCTCAAACGCTACAAGCGGCGATTATCCAACAAACCTGTTTGCACTCAGGAGGGGCCTTCCGGTTCACGGAAGGGTGCTCCAACATCTGCTCCTTATGGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001736 GO:0001738 GO:0002009 GO:0002237 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007164 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009755 GO:0009756 GO:0009787 GO:0009789 GO:0009814 GO:0009861 GO:0009871 GO:0009873 GO:0009888 GO:0009914 GO:0009926 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010053 GO:0010054 GO:0010087 GO:0010104 GO:0010119 GO:0010150 GO:0010182 GO:0010646 GO:0010647 GO:0010817 GO:0016043 GO:0021700 GO:0022622 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0035556 GO:0042221 GO:0042545 GO:0042742 GO:0043207 GO:0045087 GO:0045229 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048585 GO:0048729 GO:0048731 GO:0048764 GO:0048765 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060429 GO:0060918 GO:0065007 GO:0065008 GO:0070297 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071695 GO:0071840 GO:0080134 GO:0090558 GO:0090627 GO:0090693 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902531 GO:1905392 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
Nramp

Protein Analysis

1353

Amino Acids

146.91

Weight (kDa)

6.03

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 38 - 392 2.3e-83 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2026
Acc16I TGCGCA 3 cut(s) 2432, 2480, 2528
Acc36I ACCTGC 3 cut(s) 122, 3212, 3221
AccB1I GGYRCC 2 cut(s) 1763, 1901
AccB7I CCANNNNNTGG 3 cut(s) 1529, 3035, 3517
AccI GTMKAC 3 cut(s) 2437, 2485, 2533
AccII CGCG 1 cut(s) 2946
AciI CCGC 7 cut(s) 116, 1562, 2033, 2389, 2575, 2944, 3973
AcoI YGGCCR 2 cut(s) 2347, 3632
AcsI RAATTY 8 cut(s) 464, 1187, 1570, 2380, 2707, 2899, 3118, 3393
AcuI CTGAAG 5 cut(s) 1893, 1941, 2430, 3260, 3659
AcvI CACGTG 1 cut(s) 806
AcyI GRCGYC 1 cut(s) 2023
AfaI GTAC 9 cut(s) 297, 870, 1064, 1771, 2040, 2376, 2622, 2868, 3455
AflIII ACRYGT 2 cut(s) 389, 2955
AhdI GACNNNNNGTC 3 cut(s) 944, 3051, 3469
AjuI GAANNNNNNNTTGG 2 cut(s) 1239, 1271
AleI CACNNNNGTG 3 cut(s) 392, 1700, 3441
Alw21I GWGCWC 7 cut(s) 1046, 1390, 2320, 2974, 3043, 3642, 4036
Alw26I GTCTC 5 cut(s) 443, 1542, 1949, 3726, 3863
Alw44I GTGCAC 1 cut(s) 3039
AlwNI CAGNNNCTG 3 cut(s) 1052, 2362, 3227
Ama87I CYCGRG 2 cut(s) 2404, 2548
AoxI GGCC 6 cut(s) 1194, 2347, 2796, 3632, 3892, 4010
ApaLI GTGCAC 1 cut(s) 3039
ApoI RAATTY 8 cut(s) 464, 1187, 1570, 2380, 2707, 2899, 3118, 3393
AseI ATTAAT 1 cut(s) 3423
Asp700I GAANNNNTTC 1 cut(s) 1987
AspLEI GCGC 7 cut(s) 2418, 2433, 2466, 2481, 2514, 2529, 2562
AspS9I GGNCC 8 cut(s) 68, 325, 860, 1195, 1925, 3053, 3892, 4010
AsuII TTCGAA 2 cut(s) 3701, 3782
AsuNHI GCTAGC 1 cut(s) 11
AvaI CYCGRG 2 cut(s) 2404, 2548
AvaII GGWCC 5 cut(s) 68, 325, 860, 1925, 3053
BaeGI GKGCMC 3 cut(s) 1766, 1904, 3043
BaeI ACNNNNGTAYC 2 cut(s) 1548, 1581
BalI TGGCCA 1 cut(s) 2349
BanI GGYRCC 2 cut(s) 1763, 1901
BanII GRGCYC 3 cut(s) 1046, 1165, 3642
BarI GAAGNNNNNNTAC 8 cut(s) 1812, 1844, 2428, 2460, 2476, 2508, 2524, 2556
BbrPI CACGTG 1 cut(s) 806
BbsI GAAGAC 2 cut(s) 602, 1104
Bbv12I GWGCWC 7 cut(s) 1046, 1390, 2320, 2974, 3043, 3642, 4036
BbvCI CCTCAGC 1 cut(s) 1053
BceAI ACGGC 2 cut(s) 1093, 3921
BciVI GTATCC 4 cut(s) 1549, 2201, 3036, 3605
BclI TGATCA 1 cut(s) 1609
BcoDI GTCTC 5 cut(s) 443, 1542, 1949, 3726, 3863
BfaI CTAG 9 cut(s) 12, 887, 1734, 2019, 2030, 2103, 3150, 3414, 3768
BfmI CTRYAG 8 cut(s) 159, 222, 2220, 2734, 3091, 3204, 3228, 3831
BfoI RGCGCY 4 cut(s) 2419, 2467, 2515, 2563
BfuAI ACCTGC 3 cut(s) 122, 3212, 3221
BfuI GTATCC 4 cut(s) 1549, 2201, 3036, 3605
BglII AGATCT 3 cut(s) 238, 1170, 3560
BmcAI AGTACT 2 cut(s) 1771, 2040
Bme18I GGWCC 5 cut(s) 68, 325, 860, 1925, 3053
BmeRI GACNNNNNGTC 3 cut(s) 944, 3051, 3469
BmeT110I CYCGRG 2 cut(s) 2404, 2548
BmgT120I GGNCC 8 cut(s) 68, 325, 860, 1195, 1925, 3053, 3892, 4010
BmrI ACTGGG 3 cut(s) 570, 1423, 1769
BmtI GCTAGC 1 cut(s) 15
BmuI ACTGGG 3 cut(s) 570, 1423, 1769
BpiI GAAGAC 2 cut(s) 602, 1104
BpmI CTGGAG 4 cut(s) 467, 946, 2785, 3577
Bpu10I CCTNAGC 4 cut(s) 246, 778, 1045, 1053
Bpu14I TTCGAA 2 cut(s) 3701, 3782
BpuEI CTTGAG 1 cut(s) 2239
BsaAI YACGTR 2 cut(s) 279, 806
BsaBI GATNNNNATC 3 cut(s) 3186, 3360, 3501
BsaHI GRCGYC 1 cut(s) 2023
BsaWI WCCGGW 1 cut(s) 4016
BsaXI ACNNNNNCTCC 4 cut(s) 1933, 1963, 4030, 4060
Bse118I RCCGGY 2 cut(s) 3630, 3911
Bse3DI GCAATG 3 cut(s) 486, 837, 1008
Bse8I GATNNNNATC 3 cut(s) 3186, 3360, 3501
BseJI GATNNNNATC 3 cut(s) 3186, 3360, 3501
BseMI GCAATG 3 cut(s) 486, 837, 1008
BseRI GAGGAG 2 cut(s) 275, 1472
BseSI GKGCMC 3 cut(s) 1766, 1904, 3043
BseYI CCCAGC 2 cut(s) 1295, 2598
BsgI GTGCAG 4 cut(s) 683, 718, 1296, 2775
Bsh1236I CGCG 1 cut(s) 2946
BshFI GGCC 6 cut(s) 1196, 2349, 2798, 3634, 3894, 4012
BshNI GGYRCC 2 cut(s) 1763, 1901
BsiHKAI GWGCWC 7 cut(s) 1046, 1390, 2320, 2974, 3043, 3642, 4036
BsiHKCI CYCGRG 2 cut(s) 2404, 2548
BsiSI CCGG 3 cut(s) 3631, 3912, 4017
BslFI GGGAC 7 cut(s) 392, 820, 951, 2070, 3214, 3601, 3916
BsmAI GTCTC 5 cut(s) 443, 1542, 1949, 3726, 3863
BsmFI GGGAC 7 cut(s) 392, 820, 951, 2070, 3214, 3601, 3916
BsnI GGCC 6 cut(s) 1196, 2349, 2798, 3634, 3894, 4012
BsoBI CYCGRG 2 cut(s) 2404, 2548
Bsp119I TTCGAA 2 cut(s) 3701, 3782
Bsp1407I TGTACA 2 cut(s) 2374, 2866
Bsp19I CCATGG 2 cut(s) 2074, 2757
BspACI CCGC 7 cut(s) 116, 1562, 2033, 2389, 2575, 2944, 3973
BspANI GGCC 6 cut(s) 1196, 2349, 2798, 3634, 3894, 4012
BspFNI CGCG 1 cut(s) 2946
BspHI TCATGA 1 cut(s) 771
BspMAI CTGCAG 3 cut(s) 163, 3208, 3232
BspMI ACCTGC 3 cut(s) 122, 3212, 3221
BspOI GCTAGC 1 cut(s) 15
BspT104I TTCGAA 2 cut(s) 3701, 3782
BspT107I GGYRCC 2 cut(s) 1763, 1901
BsrDI GCAATG 3 cut(s) 486, 837, 1008
BsrFI RCCGGY 2 cut(s) 3630, 3911
BsrGI TGTACA 2 cut(s) 2374, 2866
BssAI RCCGGY 2 cut(s) 3630, 3911
BssNAI GTATAC 3 cut(s) 2438, 2486, 2534
BssNI GRCGYC 1 cut(s) 2023
BssT1I CCWWGG 3 cut(s) 2074, 2282, 2757
Bst1107I GTATAC 3 cut(s) 2438, 2486, 2534
Bst6I CTCTTC 2 cut(s) 229, 2878
BstACI GRCGYC 1 cut(s) 2023
BstAPI GCANNNNNTGC 3 cut(s) 218, 2770, 3227
BstAUI TGTACA 2 cut(s) 2374, 2866
BstBAI YACGTR 2 cut(s) 279, 806
BstBI TTCGAA 2 cut(s) 3701, 3782
BstC8I GCNNGC 5 cut(s) 13, 1359, 2013, 2613, 3632
BstDSI CCRYGG 2 cut(s) 2074, 2757
BstFNI CGCG 1 cut(s) 2946
BstH2I RGCGCY 4 cut(s) 2419, 2467, 2515, 2563
BstHHI GCGC 7 cut(s) 2418, 2433, 2466, 2481, 2514, 2529, 2562
BstMAI GTCTC 5 cut(s) 443, 1542, 1949, 3726, 3863
BstMWI GCNNNNNNNGC 9 cut(s) 218, 413, 673, 2147, 2287, 2770, 3163, 3227, 3812
BstNSI RCATGY 3 cut(s) 356, 393, 2959
BstSFI CTRYAG 8 cut(s) 159, 222, 2220, 2734, 3091, 3204, 3228, 3831
BstSLI GKGCMC 3 cut(s) 1766, 1904, 3043
BstUI CGCG 1 cut(s) 2946
BstV2I GAAGAC 2 cut(s) 602, 1104
BstX2I RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3137, 3361, 3560
BstXI CCANNNNNNTGG 3 cut(s) 445, 1702, 3642
BstYI RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3137, 3361, 3560
BstZ17I GTATAC 3 cut(s) 2438, 2486, 2534
BsuI GTATCC 4 cut(s) 1549, 2201, 3036, 3605
BsuRI GGCC 6 cut(s) 1196, 2349, 2798, 3634, 3894, 4012
BtgI CCRYGG 2 cut(s) 2074, 2757
BtsI GCAGTG 5 cut(s) 168, 262, 1323, 2187, 3289
BveI ACCTGC 3 cut(s) 122, 3212, 3221
Cac8I GCNNGC 5 cut(s) 13, 1359, 2013, 2613, 3632
CaiI CAGNNNCTG 3 cut(s) 1052, 2362, 3227
CciI TCATGA 1 cut(s) 771
CfoI GCGC 7 cut(s) 2418, 2433, 2466, 2481, 2514, 2529, 2562
Cfr10I RCCGGY 2 cut(s) 3630, 3911
Cfr13I GGNCC 8 cut(s) 68, 325, 860, 1195, 1925, 3053, 3892, 4010
CsiI ACCWGGT 1 cut(s) 1401
Csp6I GTAC 9 cut(s) 296, 869, 1063, 1770, 2039, 2375, 2621, 2867, 3454
CviQI GTAC 9 cut(s) 296, 869, 1063, 1770, 2039, 2375, 2621, 2867, 3454
DraI TTTAAA 1 cut(s) 963
DriI GACNNNNNGTC 3 cut(s) 944, 3051, 3469
EaeI YGGCCR 2 cut(s) 2347, 3632
Eam1104I CTCTTC 2 cut(s) 229, 2878
Eam1105I GACNNNNNGTC 3 cut(s) 944, 3051, 3469
EarI CTCTTC 2 cut(s) 229, 2878
Ecl136II GAGCTC 2 cut(s) 1044, 3640
Eco130I CCWWGG 3 cut(s) 2074, 2282, 2757
Eco147I AGGCCT 1 cut(s) 2798
Eco24I GRGCYC 3 cut(s) 1046, 1165, 3642
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 5 cut(s) 68, 325, 860, 1925, 3053
Eco53kI GAGCTC 2 cut(s) 1044, 3640
Eco57I CTGAAG 5 cut(s) 1893, 1941, 2430, 3260, 3659
Eco72I CACGTG 1 cut(s) 806
Eco88I CYCGRG 2 cut(s) 2404, 2548
EcoICRI GAGCTC 2 cut(s) 1044, 3640
EcoO109I RGGNCCY 2 cut(s) 3053, 4010
EcoRI GAATTC 2 cut(s) 1187, 3118
EcoRV GATATC 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 2074, 2282, 2757
EcoT22I ATGCAT 1 cut(s) 482
EcoT38I GRGCYC 3 cut(s) 1046, 1165, 3642
ErhI CCWWGG 3 cut(s) 2074, 2282, 2757
FalI AAGNNNNNCTT 6 cut(s) 534, 566, 686, 718, 2314, 2346
FaqI GGGAC 7 cut(s) 392, 820, 951, 2070, 3214, 3601, 3916
FauI CCCGC 1 cut(s) 2396
FauNDI CATATG 1 cut(s) 2311
FbaI TGATCA 1 cut(s) 1609
FblI GTMKAC 3 cut(s) 2437, 2485, 2533
FriOI GRGCYC 3 cut(s) 1046, 1165, 3642
FspBI CTAG 9 cut(s) 12, 887, 1734, 2019, 2030, 2103, 3150, 3414, 3768
FspI TGCGCA 3 cut(s) 2432, 2480, 2528
GlaI GCGC 7 cut(s) 2417, 2432, 2465, 2480, 2513, 2528, 2561
GsaI CCCAGC 2 cut(s) 1299, 2602
GsuI CTGGAG 4 cut(s) 467, 946, 2785, 3577
HaeII RGCGCY 4 cut(s) 2419, 2467, 2515, 2563
HaeIII GGCC 6 cut(s) 1196, 2349, 2798, 3634, 3894, 4012
HapII CCGG 3 cut(s) 3631, 3912, 4017
HhaI GCGC 7 cut(s) 2418, 2433, 2466, 2481, 2514, 2529, 2562
Hin1I GRCGYC 1 cut(s) 2023
Hin6I GCGC 7 cut(s) 2416, 2431, 2464, 2479, 2512, 2527, 2560
HinP1I GCGC 7 cut(s) 2416, 2431, 2464, 2479, 2512, 2527, 2560
HincII GTYRAC 4 cut(s) 536, 570, 2027, 3474
HindII GTYRAC 4 cut(s) 536, 570, 2027, 3474
HindIII AAGCTT 3 cut(s) 3269, 3302, 3577
HpaI GTTAAC 1 cut(s) 570
HpaII CCGG 3 cut(s) 3631, 3912, 4017
HpyCH4IV ACGT 4 cut(s) 278, 805, 2023, 3660
HpyF10VI GCNNNNNNNGC 9 cut(s) 218, 413, 673, 2147, 2287, 2770, 3163, 3227, 3812
HpySE526I ACGT 4 cut(s) 278, 805, 2023, 3660
Hsp92I GRCGYC 1 cut(s) 2023
HspAI GCGC 7 cut(s) 2416, 2431, 2464, 2479, 2512, 2527, 2560
KroI GCCGGC 1 cut(s) 3630
KroNI GCCGGC 1 cut(s) 3632
Ksp22I TGATCA 1 cut(s) 1609
KspAI GTTAAC 1 cut(s) 570
LmnI GCTCC 9 cut(s) 617, 1041, 1049, 1160, 1603, 1615, 2989, 4039, 4051
MabI ACCWGGT 1 cut(s) 1401
MaeI CTAG 9 cut(s) 12, 887, 1734, 2019, 2030, 2103, 3150, 3414, 3768
MaeII ACGT 4 cut(s) 278, 805, 2023, 3660
MaeIII GTNAC 5 cut(s) 197, 226, 1750, 2672, 3792
MflI RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3137, 3361, 3560
MlsI TGGCCA 1 cut(s) 2349
MluNI TGGCCA 1 cut(s) 2349
MlyI GAGTC 9 cut(s) 616, 937, 1544, 1718, 1951, 2261, 2312, 2396, 2540
MmeI TCCRAC 7 cut(s) 70, 303, 1762, 3294, 3797, 4008, 4061
Mox20I TGGCCA 1 cut(s) 2349
Mph1103I ATGCAT 1 cut(s) 482
MroNI GCCGGC 1 cut(s) 3630
MroXI GAANNNNTTC 1 cut(s) 1987
MscI TGGCCA 1 cut(s) 2349
MseI TTAA 9 cut(s) 360, 569, 920, 962, 1226, 1373, 2246, 2649, 3423
MslI CAYNNNNRTG 5 cut(s) 392, 1491, 1700, 2079, 3441
Msp20I TGGCCA 1 cut(s) 2349
MspA1I CMGCKG 2 cut(s) 1562, 3836
MspI CCGG 3 cut(s) 3631, 3912, 4017
MvnI CGCG 1 cut(s) 2946
MwoI GCNNNNNNNGC 9 cut(s) 218, 413, 673, 2147, 2287, 2770, 3163, 3227, 3812
NaeI GCCGGC 1 cut(s) 3632
NcoI CCATGG 2 cut(s) 2074, 2757
NdeI CATATG 1 cut(s) 2311
NgoMIV GCCGGC 1 cut(s) 3630
NheI GCTAGC 1 cut(s) 11
NmuCI GTSAC 2 cut(s) 197, 226
NsbI TGCGCA 3 cut(s) 2432, 2480, 2528
NsiI ATGCAT 1 cut(s) 482
NspI RCATGY 3 cut(s) 356, 393, 2959
NspV TTCGAA 2 cut(s) 3701, 3782
OliI CACNNNNGTG 3 cut(s) 392, 1700, 3441
PagI TCATGA 1 cut(s) 771
PasI CCCWGGG 1 cut(s) 2990
PceI AGGCCT 1 cut(s) 2798
PciI ACATGT 2 cut(s) 389, 2955
PcsI WCGNNNNNNNCGW 1 cut(s) 3892
PdiI GCCGGC 1 cut(s) 3632
PdmI GAANNNNTTC 1 cut(s) 1987
PflFI GACNNNGTC 1 cut(s) 2832
PflMI CCANNNNNTGG 3 cut(s) 1529, 3035, 3517
PfoI TCCNGGA 2 cut(s) 444, 1927
PleI GAGTC 9 cut(s) 615, 936, 1544, 1717, 1950, 2261, 2311, 2396, 2540
PmaCI CACGTG 1 cut(s) 806
PmlI CACGTG 1 cut(s) 806
PpsI GAGTC 9 cut(s) 615, 936, 1544, 1717, 1950, 2261, 2311, 2396, 2540
Ppu21I YACGTR 2 cut(s) 279, 806
PpuMI RGGWCCY 1 cut(s) 3053
PscI ACATGT 2 cut(s) 389, 2955
PshBI ATTAAT 1 cut(s) 3423
Psp124BI GAGCTC 2 cut(s) 1046, 3642
Psp5II RGGWCCY 1 cut(s) 3053
PspCI CACGTG 1 cut(s) 806
PspFI CCCAGC 2 cut(s) 1295, 2598
PspPI GGNCC 8 cut(s) 68, 325, 860, 1195, 1925, 3053, 3892, 4010
PspPPI RGGWCCY 1 cut(s) 3053
PsrI GAACNNNNNNTAC 2 cut(s) 3208, 3240
PstI CTGCAG 3 cut(s) 163, 3208, 3232
PstNI CAGNNNCTG 3 cut(s) 1052, 2362, 3227
PsuI RGATCY 8 cut(s) 238, 1170, 2113, 2234, 2327, 3137, 3361, 3560
PsyI GACNNNGTC 1 cut(s) 2832
PvuII CAGCTG 1 cut(s) 3836
RsaI GTAC 9 cut(s) 297, 870, 1064, 1771, 2040, 2376, 2622, 2868, 3455
RsaNI GTAC 9 cut(s) 296, 869, 1063, 1770, 2039, 2375, 2621, 2867, 3454
RseI CAYNNNNRTG 5 cut(s) 392, 1491, 1700, 2079, 3441
SacI GAGCTC 2 cut(s) 1046, 3642
SaqAI TTAA 9 cut(s) 360, 569, 920, 962, 1226, 1373, 2246, 2649, 3423
Sau96I GGNCC 8 cut(s) 68, 325, 860, 1195, 1925, 3053, 3892, 4010
ScaI AGTACT 2 cut(s) 1771, 2040
SchI GAGTC 9 cut(s) 616, 937, 1544, 1718, 1951, 2261, 2312, 2396, 2540
SexAI ACCWGGT 1 cut(s) 1401
SfcI CTRYAG 8 cut(s) 159, 222, 2220, 2734, 3091, 3204, 3228, 3831
SfuI TTCGAA 2 cut(s) 3701, 3782
SinI GGWCC 5 cut(s) 68, 325, 860, 1925, 3053
SmiMI CAYNNNNRTG 5 cut(s) 392, 1491, 1700, 2079, 3441
SmlI CTYRAG 1 cut(s) 2254
SmoI CTYRAG 1 cut(s) 2254
SseBI AGGCCT 1 cut(s) 2798
SsiI CCGC 7 cut(s) 116, 1562, 2033, 2389, 2575, 2944, 3973
SspMI CTAG 9 cut(s) 12, 887, 1734, 2019, 2030, 2103, 3150, 3414, 3768
SstI GAGCTC 2 cut(s) 1046, 3642
StuI AGGCCT 1 cut(s) 2798
StyI CCWWGG 3 cut(s) 2074, 2282, 2757
TaiI ACGT 4 cut(s) 281, 808, 2026, 3663
TaqI TCGA 8 cut(s) 1390, 2043, 2827, 3427, 3670, 3701, 3782, 3886
TatI WGTACW 7 cut(s) 295, 1769, 2038, 2374, 2620, 2866, 3453
TauI GCSGC 3 cut(s) 119, 2036, 3976
Tru1I TTAA 9 cut(s) 360, 569, 920, 962, 1226, 1373, 2246, 2649, 3423
Tru9I TTAA 9 cut(s) 360, 569, 920, 962, 1226, 1373, 2246, 2649, 3423
TseFI GTSAC 2 cut(s) 197, 226
Tsp45I GTSAC 2 cut(s) 197, 226
TspDTI ATGAA 9 cut(s) 638, 1527, 1899, 1977, 2628, 2706, 3027, 3084, 3417
TspGWI ACGGA 6 cut(s) 229, 2149, 2260, 2826, 3058, 4039
Tth111I GACNNNGTC 1 cut(s) 2832
Van91I CCANNNNNTGG 3 cut(s) 1529, 3035, 3517
VneI GTGCAC 1 cut(s) 3039
VpaK11BI GGWCC 5 cut(s) 68, 325, 860, 1925, 3053
VspI ATTAAT 1 cut(s) 3423
XapI RAATTY 8 cut(s) 464, 1187, 1570, 2380, 2707, 2899, 3118, 3393
XbaI TCTAGA 1 cut(s) 3149
XceI RCATGY 3 cut(s) 356, 393, 2959
XcmI CCANNNNNNNNNTGG 1 cut(s) 1702
XmiI GTMKAC 3 cut(s) 2437, 2485, 2533
XmnI GAANNNNTTC 1 cut(s) 1987
XspI CTAG 9 cut(s) 12, 887, 1734, 2019, 2030, 2103, 3150, 3414, 3768
ZraI GACGTC 1 cut(s) 2024
ZrmI AGTACT 2 cut(s) 1771, 2040
Zsp2I ATGCAT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.