FvH4_6g16670

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007639
Physical Location & Seq
Reverse (-)
10564991 .. 10568600
3610 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16670.t2

Sequence Viewer

Length: 1458 bp
ATGCTAACCAGAACCTGCATTGCCCTCATCTCTCTTCTCGCTTCATTCTCTCTTGCTAAAGCAGCCTACCTCAACCTCACTCTTCCAGGCCAACATCCCAACCCAGAGGAAGTTGTTCAAGAAGTTCATAGGAGGGTAAATGCTTCTGTAGTAAGAAGGGAAATGCTGCAAGTAACCCAGAGAGAGCAGTTCTCTTCATGCTTAACCGGGAACCCTATCGACGATTGCTGGAAATGCGACCCGGACTGGCCCAACAACCGTCAACGCTTAGCGGACTGCGCAATCGGGTTTGGTCAGTATGCTCTTGGAGGCAAAGGTGGGGAGTACTACATTGTCACTGACTCTTCCGACGATGATGCCGTGAATCCAAAGCCGGGGACTCTGAGATACGCCGTGATACAGCCGGAACCATTGTGGATTGTGTTCCCTAGCAACATGCTCATCAAGCTTAATCAGGAGCTCATCTTTAATAGCTACAAGACTGTTGATGGGAGAGGAGCTAATGTTCACATTACGGGCGGGGGATGCATTACTTTGCAGTACATAAGCAATGTCATCATCCACAATGTGCATATCCACCATTGTGTTCAGTCAGGGAATACAAATGTGCGGTCAAGCCCAACCCACTACGGCTACCGCACGAAGTCGGATGGCGACGGCATCTCCATCTTCGGGTCCAAAGACATATGGGTCGACCACTGCAGCCTATCACACTGCAAGGACGGTCTCATCGACGCCGTGATGGGTTCTACAGGCATCACAATCTCAAACAACTACTTCTCCAACCACAACGAGGTAATGCTCCTCGGCCACAGCGACGACTACTTGCCGGACTCCGGCATGCAGGTGACCATAGCCTTCAACCATTTCGGGGAGAATCTAGTGCAGCGCATGCCTAGGTGCAGACGCGGCTACATCCACGTAGTGAACAACGACTTCACTGCATGGGAGATGTACGCGATCGGCGGGAGTGGGAACCCTACGATAAATAGCCAGGGGAACAGGTACACTGCGCCGACGAATAGGAACGCCAAGGAGGTGACGAAGCGGGTGGACACGGGGGAGGGGCAGTGGAGAGGGTGGAACTGGCGGTCGGAGGGGGACATAATGGTAAATGGAGCCTTCTTTGTGGCGTCCGGCGCCGGTGTGGAGATCAAGTACGAGAAGGCTTACAGTGTGGAGCCTAAGTCAGCGGTGCTTATTGACCAACTAATCATGCACGCTGGTGCTCTTGGTGTTGGTGGCAGGGACAACAACCTGGGGAAGTGGAACACAGGCCCGAACCAAGGCTTCGATTCAGGGCCGGATTATGAGGATGAAATGTCCGGAAGCAGCACCAACATGCCAATGCAGCCGCTTTCATCCACCTCTACTCTCTTTACCTTTTTTATCCCATTCTCGTGCTTGTTGTTTTTGTATATCATCCCTGACTTGCATTTGATCGCAATGAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

486

Amino Acids

53.66

Weight (kDa)

5.96

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 154 - 354 3.9e-25 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 835
AasI GACNNNNNNGTC 1 cut(s) 689
Acc16I TGCGCA 1 cut(s) 280
Acc36I ACCTGC 2 cut(s) 23, 835
AccB1I GGYRCC 1 cut(s) 1139
AccI GTMKAC 1 cut(s) 693
AccII CGCG 2 cut(s) 909, 959
AccIII TCCGGA 1 cut(s) 1325
AcoI YGGCCR 1 cut(s) 808
AcyI GRCGYC 3 cut(s) 735, 1133, 1140
AdeI CACNNNGTG 2 cut(s) 568, 925
AfaI GTAC 5 cut(s) 326, 542, 956, 1007, 1160
AfiI CCNNNNNNNGG 6 cut(s) 374, 672, 793, 836, 871, 1286
AgsI TTSAA 2 cut(s) 119, 862
AjnI CCWGG 3 cut(s) 85, 993, 1257
AleI CACNNNNGTG 2 cut(s) 582, 1224
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 4 cut(s) 448, 460, 474, 500
AluI AGCT 4 cut(s) 448, 460, 474, 500
Alw21I GWGCWC 2 cut(s) 462, 1231
Alw26I GTCTC 1 cut(s) 731
AlwNI CAGNNNCTG 1 cut(s) 15
Aor13HI TCCGGA 1 cut(s) 1325
AoxI GGCC 5 cut(s) 88, 248, 808, 1276, 1301
ApeKI GCWGC 6 cut(s) 62, 166, 702, 886, 1332, 1351
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
Asp700I GAANNNNTTC 1 cut(s) 114
AspA2I CCTAGG 1 cut(s) 896
AspLEI GCGC 4 cut(s) 281, 891, 1015, 1142
AspS9I GGNCC 4 cut(s) 249, 675, 1277, 1301
AsuC2I CCSGG 3 cut(s) 208, 242, 375
AsuHPI GGTGA 2 cut(s) 859, 1051
AvaII GGWCC 1 cut(s) 675
AvrII CCTAGG 1 cut(s) 896
BanI GGYRCC 1 cut(s) 1139
BanII GRGCYC 1 cut(s) 462
BauI CACGAG 1 cut(s) 1399
Bbv12I GWGCWC 2 cut(s) 462, 1231
BbvI GCAGC 6 cut(s) 74, 153, 714, 898, 1344, 1363
BccI CCATC 4 cut(s) 482, 644, 674, 736
BceAI ACGGC 5 cut(s) 344, 377, 646, 673, 722
BcgI CGANNNNNNTGC 4 cut(s) 338, 372, 923, 957
BciT130I CCWGG 3 cut(s) 87, 995, 1259
BcnI CCSGG 3 cut(s) 208, 242, 375
BcoDI GTCTC 1 cut(s) 731
BfaI CTAG 3 cut(s) 429, 881, 897
BfmI CTRYAG 3 cut(s) 147, 700, 750
BfoI RGCGCY 1 cut(s) 1143
BfuAI ACCTGC 2 cut(s) 23, 835
BisI GCNGC 8 cut(s) 63, 167, 703, 887, 910, 1333, 1352, 1355
BlnI CCTAGG 1 cut(s) 896
BlpI GCTNAGC 1 cut(s) 268
BlsI GCNGC 8 cut(s) 64, 168, 704, 888, 911, 1334, 1353, 1356
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 6 cut(s) 87, 208, 242, 375, 995, 1259
Bme18I GGWCC 1 cut(s) 675
BmgT120I GGNCC 4 cut(s) 249, 675, 1277, 1301
BmiI GGNNCC 7 cut(s) 212, 408, 676, 977, 1120, 1141, 1182
BmrFI CCNGG 6 cut(s) 87, 208, 242, 375, 995, 1259
BmsI GCATC 4 cut(s) 346, 515, 669, 765
BplI GAGNNNNNCTC 2 cut(s) 176, 208
Bpu1102I GCTNAGC 1 cut(s) 268
BpuMI CCSGG 3 cut(s) 208, 242, 375
BsaAI YACGTR 1 cut(s) 922
BsaHI GRCGYC 3 cut(s) 735, 1133, 1140
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 7 cut(s) 374, 805, 896, 994, 1032, 1258, 1285
BsaWI WCCGGW 1 cut(s) 1325
Bsc4I CCNNNNNNNGG 6 cut(s) 374, 672, 793, 836, 871, 1286
Bse118I RCCGGY 1 cut(s) 1142
Bse1I ACTGG 2 cut(s) 251, 1091
Bse3DI GCAATG 3 cut(s) 18, 556, 1452
BseAI TCCGGA 1 cut(s) 1325
BseBI CCWGG 3 cut(s) 87, 995, 1259
BseDI CCNNGG 7 cut(s) 374, 805, 896, 994, 1032, 1258, 1285
BseGI GGATG 8 cut(s) 94, 530, 558, 655, 915, 1321, 1361, 1422
BseLI CCNNNNNNNGG 6 cut(s) 374, 672, 793, 836, 871, 1286
BseMI GCAATG 3 cut(s) 18, 556, 1452
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 2 cut(s) 251, 1091
BseRI GAGGAG 2 cut(s) 510, 794
BseXI GCAGC 6 cut(s) 74, 153, 714, 898, 1344, 1363
BsgI GTGCAG 2 cut(s) 905, 922
Bsh1236I CGCG 2 cut(s) 909, 959
Bsh1285I CGRYCG 2 cut(s) 963, 1094
BshFI GGCC 5 cut(s) 90, 250, 810, 1278, 1303
BshNI GGYRCC 1 cut(s) 1139
BsiEI CGRYCG 2 cut(s) 963, 1094
BsiHKAI GWGCWC 2 cut(s) 462, 1231
BslFI GGGAC 3 cut(s) 391, 1115, 1262
BslI CCNNNNNNNGG 6 cut(s) 374, 672, 793, 836, 871, 1286
BsmAI GTCTC 1 cut(s) 731
BsmFI GGGAC 3 cut(s) 391, 1115, 1262
BsnI GGCC 5 cut(s) 90, 250, 810, 1278, 1303
Bso31I GGTCTC 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 462, 1231
Bsp13I TCCGGA 1 cut(s) 1325
Bsp143I GATC 3 cut(s) 960, 1152, 1440
Bsp1720I GCTNAGC 1 cut(s) 268
BspANI GGCC 5 cut(s) 90, 250, 810, 1278, 1303
BspCNI CTCAG 1 cut(s) 375
BspEI TCCGGA 1 cut(s) 1325
BspFNI CGCG 2 cut(s) 909, 959
BspLI GGNNCC 7 cut(s) 212, 408, 676, 977, 1120, 1141, 1182
BspMAI CTGCAG 1 cut(s) 704
BspMI ACCTGC 2 cut(s) 23, 835
BspT107I GGYRCC 1 cut(s) 1139
BspTNI GGTCTC 1 cut(s) 731
BsrDI GCAATG 3 cut(s) 18, 556, 1452
BsrFI RCCGGY 1 cut(s) 1142
BsrI ACTGG 2 cut(s) 251, 1091
BssAI RCCGGY 1 cut(s) 1142
BssECI CCNNGG 7 cut(s) 374, 805, 896, 994, 1032, 1258, 1285
BssMI GATC 3 cut(s) 960, 1152, 1440
BssNI GRCGYC 3 cut(s) 735, 1133, 1140
BssSI CACGAG 1 cut(s) 1399
BssT1I CCWWGG 3 cut(s) 896, 1032, 1285
Bst2BI CACGAG 1 cut(s) 1399
Bst2UI CCWGG 3 cut(s) 87, 995, 1259
Bst4CI ACNGT 4 cut(s) 260, 484, 725, 1175
Bst6I CTCTTC 4 cut(s) 39, 87, 199, 349
BstACI GRCGYC 3 cut(s) 735, 1133, 1140
BstAPI GCANNNNNTGC 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 922
BstC8I GCNNGC 3 cut(s) 842, 893, 1221
BstDEI CTNAG 3 cut(s) 268, 383, 1185
BstEII GGTNACC 1 cut(s) 847
BstF5I GGATG 8 cut(s) 94, 530, 558, 655, 915, 1321, 1361, 1422
BstFNI CGCG 2 cut(s) 909, 959
BstH2I RGCGCY 1 cut(s) 1143
BstHHI GCGC 4 cut(s) 281, 891, 1015, 1142
BstKTI GATC 3 cut(s) 963, 1155, 1443
BstMAI GTCTC 1 cut(s) 731
BstMBI GATC 3 cut(s) 960, 1152, 1440
BstMCI CGRYCG 2 cut(s) 963, 1094
BstMWI GCNNNNNNNGC 9 cut(s) 62, 234, 278, 445, 525, 892, 909, 1139, 1351
BstNI CCWGG 3 cut(s) 87, 995, 1259
BstNSI RCATGY 4 cut(s) 439, 844, 895, 1345
BstPI GGTNACC 1 cut(s) 847
BstSCI CCNGG 6 cut(s) 85, 206, 240, 373, 993, 1257
BstSFI CTRYAG 3 cut(s) 147, 700, 750
BstUI CGCG 2 cut(s) 909, 959
BstV1I GCAGC 6 cut(s) 74, 153, 714, 898, 1344, 1363
BsuRI GGCC 5 cut(s) 90, 250, 810, 1278, 1303
BtsCI GGATG 8 cut(s) 94, 530, 558, 655, 915, 1321, 1361, 1422
BtsI GCAGTG 5 cut(s) 697, 712, 939, 1008, 1076
BtsIMutI CAGTG 7 cut(s) 336, 697, 712, 939, 1008, 1076, 1180
BveI ACCTGC 2 cut(s) 23, 835
Cac8I GCNNGC 3 cut(s) 842, 893, 1221
CaiI CAGNNNCTG 1 cut(s) 15
CfoI GCGC 4 cut(s) 281, 891, 1015, 1142
Cfr10I RCCGGY 1 cut(s) 1142
Cfr13I GGNCC 4 cut(s) 249, 675, 1277, 1301
CseI GACGC 3 cut(s) 743, 915, 1122
Csp6I GTAC 5 cut(s) 325, 541, 955, 1006, 1159
CviAII CATG 7 cut(s) 198, 436, 841, 892, 945, 1216, 1342
CviQI GTAC 5 cut(s) 325, 541, 955, 1006, 1159
DdeI CTNAG 3 cut(s) 268, 383, 1185
DinI GGCGCC 1 cut(s) 1141
DpnI GATC 3 cut(s) 962, 1154, 1442
DpnII GATC 3 cut(s) 960, 1152, 1440
DraIII CACNNNGTG 2 cut(s) 568, 925
DrdI GACNNNNNNGTC 1 cut(s) 689
DseDI GACNNNNNNGTC 1 cut(s) 689
EaeI YGGCCR 1 cut(s) 808
Eam1104I CTCTTC 4 cut(s) 39, 87, 199, 349
EarI CTCTTC 4 cut(s) 39, 87, 199, 349
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 3 cut(s) 896, 1032, 1285
Eco24I GRGCYC 1 cut(s) 462
Eco31I GGTCTC 1 cut(s) 731
Eco47I GGWCC 1 cut(s) 675
Eco53kI GAGCTC 1 cut(s) 460
Eco91I GGTNACC 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 460
EcoO65I GGTNACC 1 cut(s) 847
EcoRII CCWGG 3 cut(s) 85, 993, 1257
EcoT14I CCWWGG 3 cut(s) 896, 1032, 1285
EcoT22I ATGCAT 1 cut(s) 530
EcoT38I GRGCYC 1 cut(s) 462
EgeI GGCGCC 1 cut(s) 1141
EheI GGCGCC 1 cut(s) 1141
ErhI CCWWGG 3 cut(s) 896, 1032, 1285
FaeI CATG 7 cut(s) 201, 439, 844, 895, 948, 1219, 1345
FaqI GGGAC 3 cut(s) 391, 1115, 1262
FatI CATG 7 cut(s) 197, 435, 840, 891, 944, 1215, 1341
FauI CCCGC 3 cut(s) 512, 959, 1041
FauNDI CATATG 1 cut(s) 686
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 8 cut(s) 63, 167, 703, 887, 910, 1333, 1352, 1355
FokI GGATG 8 cut(s) 81, 537, 545, 662, 902, 1328, 1348, 1409
FriOI GRGCYC 1 cut(s) 462
Fsp4HI GCNGC 8 cut(s) 63, 167, 703, 887, 910, 1333, 1352, 1355
FspBI CTAG 3 cut(s) 429, 881, 897
FspI TGCGCA 1 cut(s) 280
GlaI GCGC 4 cut(s) 280, 890, 1014, 1141
GluI GCNGC 8 cut(s) 63, 167, 703, 887, 910, 1333, 1352, 1355
HaeII RGCGCY 1 cut(s) 1143
HaeIII GGCC 5 cut(s) 90, 250, 810, 1278, 1303
HgaI GACGC 3 cut(s) 743, 915, 1122
HhaI GCGC 4 cut(s) 281, 891, 1015, 1142
Hin1I GRCGYC 3 cut(s) 735, 1133, 1140
Hin1II CATG 7 cut(s) 201, 439, 844, 895, 948, 1219, 1345
Hin6I GCGC 4 cut(s) 279, 889, 1013, 1140
HinP1I GCGC 4 cut(s) 279, 889, 1013, 1140
HincII GTYRAC 2 cut(s) 263, 694
HindII GTYRAC 2 cut(s) 263, 694
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 6 cut(s) 341, 364, 379, 833, 877, 1295
HphI GGTGA 2 cut(s) 859, 1051
Hpy166II GTNNAC 6 cut(s) 263, 508, 694, 928, 1008, 1054
Hpy188I TCNGA 4 cut(s) 349, 384, 649, 1096
Hpy188III TCNNGA 3 cut(s) 119, 455, 1326
Hpy8I GTNNAC 6 cut(s) 263, 508, 694, 928, 1008, 1054
Hpy99I CGWCG 6 cut(s) 224, 353, 659, 737, 821, 1021
HpyAV CCTTC 4 cut(s) 150, 868, 1132, 1159
HpyCH4III ACNGT 4 cut(s) 260, 484, 725, 1175
HpyCH4IV ACGT 1 cut(s) 921
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 234, 278, 445, 525, 892, 909, 1139, 1351
HpyF3I CTNAG 3 cut(s) 268, 383, 1185
HpySE526I ACGT 1 cut(s) 921
Hsp92I GRCGYC 3 cut(s) 735, 1133, 1140
Hsp92II CATG 7 cut(s) 201, 439, 844, 895, 948, 1219, 1345
HspAI GCGC 4 cut(s) 279, 889, 1013, 1140
KasI GGCGCC 1 cut(s) 1139
Kpn2I TCCGGA 1 cut(s) 1325
Kzo9I GATC 3 cut(s) 960, 1152, 1440
LmnI GCTCC 5 cut(s) 457, 497, 807, 1118, 1180
Lsp1109I GCAGC 6 cut(s) 74, 153, 714, 898, 1344, 1363
LweI GCATC 4 cut(s) 346, 515, 669, 765
MaeI CTAG 3 cut(s) 429, 881, 897
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 4 cut(s) 172, 334, 847, 1039
MalI GATC 3 cut(s) 962, 1154, 1442
MboI GATC 3 cut(s) 960, 1152, 1440
MboII GAAGA 5 cut(s) 26, 74, 186, 336, 661
MhlI GDGCHC 2 cut(s) 462, 1231
MluCI AATT 1 cut(s) 1453
Mly113I GGCGCC 1 cut(s) 1140
MlyI GAGTC 3 cut(s) 335, 373, 827
MmeI TCCRAC 4 cut(s) 372, 627, 807, 1074
Mph1103I ATGCAT 1 cut(s) 530
MroI TCCGGA 1 cut(s) 1325
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 4 cut(s) 203, 450, 468, 1456
MslI CAYNNNNRTG 6 cut(s) 582, 845, 1224, 1340, 1346, 1399
MspA1I CMGCKG 1 cut(s) 1193
MspR9I CCNGG 6 cut(s) 87, 208, 242, 375, 995, 1259
MvaI CCWGG 3 cut(s) 87, 995, 1259
MvnI CGCG 2 cut(s) 909, 959
MwoI GCNNNNNNNGC 9 cut(s) 62, 234, 278, 445, 525, 892, 909, 1139, 1351
NarI GGCGCC 1 cut(s) 1140
NciI CCSGG 3 cut(s) 208, 242, 375
NdeI CATATG 1 cut(s) 686
NdeII GATC 3 cut(s) 960, 1152, 1440
NlaIII CATG 7 cut(s) 201, 439, 844, 895, 948, 1219, 1345
NlaIV GGNNCC 7 cut(s) 212, 408, 676, 977, 1120, 1141, 1182
NmeAIII GCCGAG 1 cut(s) 786
NmuCI GTSAC 3 cut(s) 334, 847, 1039
NsbI TGCGCA 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 530
NspI RCATGY 4 cut(s) 439, 844, 895, 1345
OliI CACNNNNGTG 2 cut(s) 582, 1224
PaeI GCATGC 2 cut(s) 844, 895
PaqCI CACCTGC 1 cut(s) 835
PcsI WCGNNNNNNNCGW 3 cut(s) 357, 729, 813
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 3 cut(s) 364, 877, 1295
PkrI GCNGC 8 cut(s) 64, 168, 704, 888, 911, 1334, 1353, 1356
Ple19I CGATCG 1 cut(s) 963
PleI GAGTC 3 cut(s) 335, 373, 827
PluTI GGCGCC 1 cut(s) 1143
PpsI GAGTC 3 cut(s) 335, 373, 827
Ppu21I YACGTR 1 cut(s) 922
Psp124BI GAGCTC 1 cut(s) 462
Psp6I CCWGG 3 cut(s) 85, 993, 1257
PspEI GGTNACC 1 cut(s) 847
PspGI CCWGG 3 cut(s) 85, 993, 1257
PspN4I GGNNCC 7 cut(s) 212, 408, 676, 977, 1120, 1141, 1182
PspPI GGNCC 4 cut(s) 249, 675, 1277, 1301
PstI CTGCAG 1 cut(s) 704
PstNI CAGNNNCTG 1 cut(s) 15
PvuI CGATCG 1 cut(s) 963
RsaI GTAC 5 cut(s) 326, 542, 956, 1007, 1160
RsaNI GTAC 5 cut(s) 325, 541, 955, 1006, 1159
RseI CAYNNNNRTG 6 cut(s) 582, 845, 1224, 1340, 1346, 1399
SacI GAGCTC 1 cut(s) 462
SalI GTCGAC 1 cut(s) 692
SaqAI TTAA 4 cut(s) 203, 450, 468, 1456
SatI GCNGC 8 cut(s) 63, 167, 703, 887, 910, 1333, 1352, 1355
Sau3AI GATC 3 cut(s) 960, 1152, 1440
Sau96I GGNCC 4 cut(s) 249, 675, 1277, 1301
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 3 cut(s) 335, 373, 827
ScrFI CCNGG 6 cut(s) 87, 208, 242, 375, 995, 1259
SduI GDGCHC 2 cut(s) 462, 1231
SfaNI GCATC 4 cut(s) 346, 515, 669, 765
SfcI CTRYAG 3 cut(s) 147, 700, 750
SfoI GGCGCC 1 cut(s) 1141
SgrAI CRCCGGYG 1 cut(s) 1142
SinI GGWCC 1 cut(s) 675
SmiMI CAYNNNNRTG 6 cut(s) 582, 845, 1224, 1340, 1346, 1399
SphI GCATGC 2 cut(s) 844, 895
Sse9I AATT 1 cut(s) 1453
SspDI GGCGCC 1 cut(s) 1139
SspMI CTAG 3 cut(s) 429, 881, 897
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 6 cut(s) 85, 206, 240, 373, 993, 1257
StyI CCWWGG 3 cut(s) 896, 1032, 1285
TaaI ACNGT 4 cut(s) 260, 484, 725, 1175
TaiI ACGT 1 cut(s) 924
TaqI TCGA 4 cut(s) 219, 693, 732, 1293
TasI AATT 1 cut(s) 1453
TatI WGTACW 2 cut(s) 324, 540
TauI GCSGC 2 cut(s) 912, 1357
TfiI GAWTC 3 cut(s) 364, 877, 1295
Tru1I TTAA 4 cut(s) 203, 450, 468, 1456
Tru9I TTAA 4 cut(s) 203, 450, 468, 1456
TscAI CASTG 7 cut(s) 343, 704, 719, 946, 1015, 1076, 1180
TseFI GTSAC 3 cut(s) 334, 847, 1039
TseI GCWGC 6 cut(s) 62, 166, 702, 886, 1332, 1351
Tsp45I GTSAC 3 cut(s) 334, 847, 1039
TspDTI ATGAA 5 cut(s) 33, 116, 186, 1332, 1350
TspRI CASTG 7 cut(s) 343, 704, 719, 946, 1015, 1076, 1180
VpaK11BI GGWCC 1 cut(s) 675
XceI RCATGY 4 cut(s) 439, 844, 895, 1345
XmaJI CCTAGG 1 cut(s) 896
XmiI GTMKAC 1 cut(s) 693
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 3 cut(s) 429, 881, 897
ZrmI AGTACT 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.