pycom04g08480

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007639
Physical Location & Seq
Forward (+)
10188842 .. 10190492
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08480.1

Sequence Viewer

Length: 699 bp
ATGATCCCCCGAACCTGCATTGTCCTGCTCTTTCTTCTCACCTCATTTTCTCCTCTTGCCTCAGCATTCGTCAACCTCACTCTCCCTGGTCAGCATCCCAACCCAGATGAAGTTGTTCTACAAGTTAATAGGAAGGTTAATGCTTCATTAGCAAGAAGGCAAATGCTGCAAACAACCCAGAAAGACCAGCTGGATTCTGCATGCTTAACTGGTAACCCGATCGACGACTGCTGGAAATGTGATCCAGATTGGCCAAACAACAGGCAAAGCCTGGCAGATTGTGCAATAGGGTTTGGCCAATATGCTCATGGAGGCAAAGGTGGTGAGTATTACATTGTGACGGACTCTTCGGATGACGATGCCGTGACTCCGAGGCCAGGCACTCTCAGATATGCTGTGATTCAGACTGAGCCACTCTGGATTGTGTTCCCTGGCAACATGCTCATCAAGCTCTCCCAAGAACTCATATTCAACAGCTACAAAACCCTCGATGGCCGCGGAGCCAATGTCCACATTGTGGGTGGTGGATGCATCACTCTGCAGTACATAAGCAATGTCATCATCCACAATGTGCATATCCACCATTGTCATCAGTCAGGTCAGGCTAATGTGCGTTCAAGCCCTACTCACTATGGCTTTCGCACAGAATCAGACGGTGATGGGATCGATCCATCTTTGGATCCAAAGACATATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

233

Amino Acids

25.51

Weight (kDa)

5.94

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 517
AccII CGCG 1 cut(s) 498
AciI CCGC 2 cut(s) 496, 498
AclWI GGATC 5 cut(s) 236, 662, 671, 674, 687
AcoI YGGCCR 3 cut(s) 251, 295, 493
AdeI CACNNNGTG 2 cut(s) 517, 571
AfaI GTAC 1 cut(s) 545
AfiI CCNNNNNNNGG 2 cut(s) 377, 517
AgsI TTSAA 2 cut(s) 472, 618
AjnI CCWGG 4 cut(s) 85, 270, 376, 430
AluBI AGCT 3 cut(s) 190, 451, 477
AluI AGCT 3 cut(s) 190, 451, 477
AlwI GGATC 5 cut(s) 236, 662, 671, 674, 687
AoxI GGCC 4 cut(s) 251, 295, 374, 493
ApeKI GCWGC 1 cut(s) 166
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
Asp700I GAANNNNTTC 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 31, 335, 668
BalI TGGCCA 2 cut(s) 253, 297
BamHI GGATCC 1 cut(s) 679
BarI GAAGNNNNNNTAC 2 cut(s) 102, 134
BbvCI CCTCAGC 1 cut(s) 61
BbvI GCAGC 1 cut(s) 153
BccI CCATC 3 cut(s) 485, 653, 679
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 4 cut(s) 87, 272, 378, 432
BfmI CTRYAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 23
BisI GCNGC 2 cut(s) 167, 496
BlsI GCNGC 2 cut(s) 168, 497
Bme1390I CCNGG 4 cut(s) 87, 272, 378, 432
BmiI GGNNCC 2 cut(s) 502, 681
BmrFI CCNGG 4 cut(s) 87, 272, 378, 432
BmsI GCATC 4 cut(s) 103, 349, 518, 540
Bpu10I CCTNAGC 1 cut(s) 61
Bsa29I ATCGAT 1 cut(s) 666
BsaJI CCNNGG 4 cut(s) 85, 371, 430, 496
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 517
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 4 cut(s) 87, 272, 378, 432
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 4 cut(s) 85, 371, 430, 496
BseGI GGATG 4 cut(s) 94, 358, 533, 561
BseLI CCNNNNNNNGG 2 cut(s) 377, 517
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 3 cut(s) 75, 399, 400
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 498
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 4 cut(s) 253, 297, 376, 495
BshVI ATCGAT 1 cut(s) 666
BsiEI CGRYCG 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 377, 517
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 4 cut(s) 253, 297, 376, 495
Bsp143I GATC 6 cut(s) 3, 219, 241, 663, 667, 679
BspACI CCGC 2 cut(s) 496, 498
BspANI GGCC 4 cut(s) 253, 297, 376, 495
BspCNI CTCAG 3 cut(s) 74, 399, 400
BspDI ATCGAT 1 cut(s) 666
BspFNI CGCG 1 cut(s) 498
BspLI GGNNCC 2 cut(s) 502, 681
BspMAI CTGCAG 1 cut(s) 543
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 5 cut(s) 236, 662, 671, 674, 687
BsrDI GCAATG 1 cut(s) 559
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 85, 371, 430, 496
BssMI GATC 6 cut(s) 3, 219, 241, 663, 667, 679
Bst2UI CCWGG 4 cut(s) 87, 272, 378, 432
Bst4CI ACNGT 1 cut(s) 656
Bst6I CTCTTC 1 cut(s) 352
BstAPI GCANNNNNTGC 2 cut(s) 166, 281
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 3 cut(s) 61, 386, 408
BstDSI CCRYGG 1 cut(s) 496
BstEII GGTNACC 1 cut(s) 212
BstF5I GGATG 4 cut(s) 94, 358, 533, 561
BstFNI CGCG 1 cut(s) 498
BstKTI GATC 6 cut(s) 6, 222, 244, 666, 670, 682
BstMBI GATC 6 cut(s) 3, 219, 241, 663, 667, 679
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 149, 166, 281, 448
BstNI CCWGG 4 cut(s) 87, 272, 378, 432
BstNSI RCATGY 2 cut(s) 204, 442
BstPI GGTNACC 1 cut(s) 212
BstSCI CCNGG 4 cut(s) 85, 270, 376, 430
BstSFI CTRYAG 1 cut(s) 539
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
Bsu15I ATCGAT 1 cut(s) 666
BsuRI GGCC 4 cut(s) 253, 297, 376, 495
BsuTUI ATCGAT 1 cut(s) 666
BtgI CCRYGG 1 cut(s) 496
BtsCI GGATG 4 cut(s) 94, 358, 533, 561
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 1 cut(s) 202
Cfr42I CCGCGG 1 cut(s) 499
ClaI ATCGAT 1 cut(s) 666
Csp6I GTAC 1 cut(s) 544
CviAII CATG 3 cut(s) 201, 308, 439
CviQI GTAC 1 cut(s) 544
DdeI CTNAG 3 cut(s) 61, 386, 408
DpnI GATC 6 cut(s) 5, 221, 243, 665, 669, 681
DpnII GATC 6 cut(s) 3, 219, 241, 663, 667, 679
DraIII CACNNNGTG 2 cut(s) 517, 571
EaeI YGGCCR 3 cut(s) 251, 295, 493
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
Eco91I GGTNACC 1 cut(s) 212
EcoO65I GGTNACC 1 cut(s) 212
EcoRII CCWGG 4 cut(s) 85, 270, 376, 430
EcoT22I ATGCAT 1 cut(s) 533
FaeI CATG 3 cut(s) 204, 311, 442
FatI CATG 3 cut(s) 200, 307, 438
FauNDI CATATG 1 cut(s) 691
Fnu4HI GCNGC 2 cut(s) 167, 496
FokI GGATG 4 cut(s) 81, 365, 540, 548
Fsp4HI GCNGC 2 cut(s) 167, 496
GluI GCNGC 2 cut(s) 167, 496
HaeIII GGCC 4 cut(s) 253, 297, 376, 495
Hin1II CATG 3 cut(s) 204, 311, 442
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 5 cut(s) 194, 344, 367, 400, 647
HphI GGTGA 3 cut(s) 31, 335, 668
Hpy166II GTNNAC 2 cut(s) 73, 511
Hpy188I TCNGA 5 cut(s) 352, 372, 389, 405, 652
Hpy188III TCNNGA 2 cut(s) 245, 418
Hpy8I GTNNAC 2 cut(s) 73, 511
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 2 cut(s) 127, 150
HpyCH4III ACNGT 1 cut(s) 656
HpyCH4V TGCA 7 cut(s) 18, 169, 200, 284, 531, 541, 574
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 166, 281, 448
HpyF3I CTNAG 3 cut(s) 61, 386, 408
Hsp92II CATG 3 cut(s) 204, 311, 442
KspI CCGCGG 1 cut(s) 499
Kzo9I GATC 6 cut(s) 3, 219, 241, 663, 667, 679
LmnI GCTCC 1 cut(s) 500
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 4 cut(s) 103, 349, 518, 540
MaeIII GTNAC 3 cut(s) 212, 337, 364
MalI GATC 6 cut(s) 5, 221, 243, 665, 669, 681
MboI GATC 6 cut(s) 3, 219, 241, 663, 667, 679
MboII GAAGA 2 cut(s) 26, 339
MflI RGATCY 1 cut(s) 679
MlsI TGGCCA 2 cut(s) 253, 297
MluNI TGGCCA 2 cut(s) 253, 297
MlyI GAGTC 2 cut(s) 338, 361
MnlI CCTC 7 cut(s) 52, 63, 70, 86, 305, 366, 497
Mox20I TGGCCA 2 cut(s) 253, 297
Mph1103I ATGCAT 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 114
MscI TGGCCA 2 cut(s) 253, 297
MseI TTAA 3 cut(s) 126, 138, 206
Msp20I TGGCCA 2 cut(s) 253, 297
MspA1I CMGCKG 2 cut(s) 190, 498
MspR9I CCNGG 4 cut(s) 87, 272, 378, 432
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 4 cut(s) 87, 272, 378, 432
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 4 cut(s) 149, 166, 281, 448
NdeI CATATG 1 cut(s) 691
NdeII GATC 6 cut(s) 3, 219, 241, 663, 667, 679
NlaIII CATG 3 cut(s) 204, 311, 442
NlaIV GGNNCC 2 cut(s) 502, 681
NmuCI GTSAC 2 cut(s) 337, 364
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 2 cut(s) 204, 442
PaeI GCATGC 1 cut(s) 204
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 3 cut(s) 194, 400, 647
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 2 cut(s) 168, 497
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 2 cut(s) 338, 361
PpsI GAGTC 2 cut(s) 338, 361
Psp6I CCWGG 4 cut(s) 85, 270, 376, 430
PspEI GGTNACC 1 cut(s) 212
PspGI CCWGG 4 cut(s) 85, 270, 376, 430
PspN4I GGNNCC 2 cut(s) 502, 681
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 1 cut(s) 679
PvuI CGATCG 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 1 cut(s) 545
RsaNI GTAC 1 cut(s) 544
SacII CCGCGG 1 cut(s) 499
SaqAI TTAA 3 cut(s) 126, 138, 206
SatI GCNGC 2 cut(s) 167, 496
Sau3AI GATC 6 cut(s) 3, 219, 241, 663, 667, 679
SchI GAGTC 2 cut(s) 338, 361
ScrFI CCNGG 4 cut(s) 87, 272, 378, 432
SetI ASST 9 cut(s) 17, 44, 78, 138, 192, 322, 453, 479, 601
SfaNI GCATC 4 cut(s) 103, 349, 518, 540
SfcI CTRYAG 1 cut(s) 539
Sfr303I CCGCGG 1 cut(s) 499
SgrBI CCGCGG 1 cut(s) 499
SphI GCATGC 1 cut(s) 204
SsiI CCGC 2 cut(s) 496, 498
StyD4I CCNGG 4 cut(s) 85, 270, 376, 430
TaaI ACNGT 1 cut(s) 656
TaqI TCGA 3 cut(s) 222, 489, 666
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 3 cut(s) 194, 400, 647
Tru1I TTAA 3 cut(s) 126, 138, 206
Tru9I TTAA 3 cut(s) 126, 138, 206
TseFI GTSAC 2 cut(s) 337, 364
TseI GCWGC 1 cut(s) 166
Tsp45I GTSAC 2 cut(s) 337, 364
TspDTI ATGAA 2 cut(s) 123, 135
TspGWI ACGGA 1 cut(s) 356
Van91I CCANNNNNTGG 1 cut(s) 517
XceI RCATGY 2 cut(s) 204, 442
XcmI CCANNNNNNNNNTGG 2 cut(s) 305, 518
XmnI GAANNNNTTC 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.