MD12G1108300.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007639
Physical Location & Seq
Forward (+)
17124977 .. 17128832
3856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1108300.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGATCCCCAGAACCTGCATTGTCCTGCTTTCTCTTCTCACCTCATTTTCCCCTCTTGCCACTGCATTCCTCAACCTCACTCTCCCTGGTCAGCATCCCAACCCAGATGAAGTTGTTCTACAAGTTAATAGGAAGGTTAATGCTTCGTTAGAAAGAAGGCAAATGCTGCAAACAACCCAGAAAGACAATCTGGATTCTACGTGCCTGACCGGAAACCCGATAGACGACTGCTGGAAATGTGATCCGGATTGGCCAAACAACAGGCAAAGCCTGGCAGATTGTGCAATAGGGTTTGGTCAATATGCTCATGGAGGCAAAGGGGGTGAGTATTACATTGTGACGGACGACTCAGATGACGACGCGGTGACTCCGAAGCCAGGCACTCTCAGATATGCTGTGATTCAGACTGAGCCTCTCTGGATTGTGTTCCCTCGCAACATGCTCATCAAGCTCTCCCAAGAACTTATCTTCAACAGCTACAAAACCCTCGATGGCCGCGGAGCGAATGTCCACATTGTTGGTGGTGGATGCATCACTCTGCAGTACATAAGCAATGTCATCATCCACAATGTGCATATCCACCATTGTTATCAGTCAGGTCAGGCTAATGTGCGTTCCAGCCCAACTCACTACGGCTTTCGCACAGAATCAGACGGCGATGGGATCTCCATCTTTGGATCCAAAGACATATGGATAGACCATTGTAGCCTATCACACTGCAAGGATGGACTGATAGATGCAGTGATGGGGTCCACAGGAATAACAATATCAAACAATTATTTCTCTCATCACAACGAAGTCATGCTGCTTGGTCACAGCGATGACTACTTGCCCGACTCTGGAATGCAAGTCACCATATCCTTCAATCACTTTGGAGAAAAGTTGGTGCAGAGAATGCCTAGGTGTAGAAGGGGCTACATCCACGTGGTCAACAACGACTTCACCCAATGGGAAATGTACGCCATCGGAGGCAGTGGAGACCCTACAATTAACAGCCAGGGCAACCGCTACACTGCTCCGTCAAACCCTAACGCCAAGGAGGTGACAAAGCGTGTGGAGACGGCGGAAGGGAAGTGGAGGAACTGGAACTGGAGATCGGAGGGGGACATAATGGTAAACGGAGCATTTTTTGTAGCATCTGGGGAGGGAGTGGAGCTTAACTATGAGAAGGCCTACAGTGTGGAGCCCAAGTCTGCTGCGCTTATTGACCAACTCATCATGCATGCTGGTGCCCTTGGTGTTGGTGGCAGGGACAACAACCTGGGGAAGTGGAGCTCGAACGGCGACGGAAATGGTTTAGGCTCAGGTCCAGACTATACGGATGACATGTCGGGAAGCAGCCGTATTCCACAGCCGCCACTTTCATCCACCTCCACCATCTTCTCATTTTTCATCTCGTTGTCATGCTTGCTATTTTTGTATATTATCCCAGACATGCATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

482

Amino Acids

53.07

Weight (kDa)

5.6

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 155 - 411 2.6e-24 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1444
Acc36I ACCTGC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 1229
AccII CGCG 2 cut(s) 362, 498
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 6 cut(s) 362, 496, 498, 1006, 1064, 1355
AclWI GGATC 4 cut(s) 236, 671, 672, 685
AcoI YGGCCR 2 cut(s) 251, 493
AcvI CACGTG 1 cut(s) 925
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 2 cut(s) 545, 959
AfiI CCNNNNNNNGG 2 cut(s) 377, 839
AflIII ACRYGT 1 cut(s) 1326
AgsI TTSAA 2 cut(s) 472, 865
AjnI CCWGG 5 cut(s) 85, 270, 376, 996, 1260
AluBI AGCT 4 cut(s) 451, 477, 1156, 1275
AluI AGCT 4 cut(s) 451, 477, 1156, 1275
Alw21I GWGCWC 1 cut(s) 1277
Alw26I GTCTC 2 cut(s) 972, 1052
AlwI GGATC 4 cut(s) 236, 671, 672, 685
AlwNI CAGNNNCTG 1 cut(s) 15
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 3 cut(s) 251, 493, 1170
ApeKI GCWGC 4 cut(s) 166, 805, 1196, 1338
Asp700I GAANNNNTTC 1 cut(s) 114
AspA2I CCTAGG 1 cut(s) 899
AspLEI GCGC 1 cut(s) 1201
AspS9I GGNCC 2 cut(s) 750, 1307
AsuHPI GGTGA 6 cut(s) 31, 335, 376, 844, 934, 1054
AvaII GGWCC 2 cut(s) 750, 1307
AvrII CCTAGG 1 cut(s) 899
BaeGI GKGCMC 1 cut(s) 1234
BalI TGGCCA 1 cut(s) 253
BamHI GGATCC 1 cut(s) 677
BanI GGYRCC 1 cut(s) 1229
BanII GRGCYC 2 cut(s) 1188, 1277
BarI GAAGNNNNNNTAC 2 cut(s) 102, 134
BbrPI CACGTG 1 cut(s) 925
Bbv12I GWGCWC 1 cut(s) 1277
BbvI GCAGC 4 cut(s) 153, 792, 1183, 1350
BccI CCATC 7 cut(s) 485, 653, 677, 719, 739, 971, 1385
BceAI ACGGC 5 cut(s) 649, 670, 1077, 1297, 1326
BciT130I CCWGG 5 cut(s) 87, 272, 378, 998, 1262
BcoDI GTCTC 2 cut(s) 972, 1052
BfaI CTAG 1 cut(s) 900
BfmI CTRYAG 2 cut(s) 539, 1174
BfuAI ACCTGC 1 cut(s) 23
BisI GCNGC 6 cut(s) 167, 496, 806, 1197, 1339, 1355
BlnI CCTAGG 1 cut(s) 899
BlsI GCNGC 6 cut(s) 168, 497, 807, 1198, 1340, 1356
Bme1390I CCNGG 5 cut(s) 87, 272, 378, 998, 1262
Bme18I GGWCC 2 cut(s) 750, 1307
BmgT120I GGNCC 2 cut(s) 750, 1307
BmiI GGNNCC 4 cut(s) 679, 751, 1185, 1231
BmrFI CCNGG 5 cut(s) 87, 272, 378, 998, 1262
BmsI GCATC 5 cut(s) 103, 518, 540, 727, 1145
BpmI CTGGAG 1 cut(s) 1111
Bpu10I CCTNAGC 1 cut(s) 1303
BsaAI YACGTR 2 cut(s) 201, 925
BsaBI GATNNNNATC 1 cut(s) 668
BsaI GGTCTC 1 cut(s) 972
BsaJI CCNNGG 7 cut(s) 85, 496, 899, 997, 1035, 1234, 1261
BsaWI WCCGGW 2 cut(s) 209, 244
BsaXI ACNNNNNCTCC 4 cut(s) 492, 522, 1175, 1205
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 839
Bse1I ACTGG 2 cut(s) 1088, 1094
Bse3DI GCAATG 1 cut(s) 559
Bse8I GATNNNNATC 1 cut(s) 668
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 5 cut(s) 87, 272, 378, 998, 1262
BseDI CCNNGG 7 cut(s) 85, 496, 899, 997, 1035, 1234, 1261
BseGI GGATG 7 cut(s) 94, 533, 561, 730, 918, 1327, 1364
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 2 cut(s) 377, 839
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 4 cut(s) 363, 399, 400, 1317
BseNI ACTGG 2 cut(s) 1088, 1094
BseSI GKGCMC 1 cut(s) 1234
BseXI GCAGC 4 cut(s) 153, 792, 1183, 1350
BsgI GTGCAG 1 cut(s) 908
Bsh1236I CGCG 2 cut(s) 362, 498
BshFI GGCC 3 cut(s) 253, 495, 1172
BshNI GGYRCC 1 cut(s) 1229
BsiHKAI GWGCWC 1 cut(s) 1277
BsiSI CCGG 2 cut(s) 210, 245
BslFI GGGAC 2 cut(s) 1118, 1265
BslI CCNNNNNNNGG 2 cut(s) 377, 839
BsmAI GTCTC 2 cut(s) 972, 1052
BsmBI CGTCTC 1 cut(s) 1052
BsmFI GGGAC 2 cut(s) 1118, 1265
BsmI GAATGC 3 cut(s) 65, 849, 900
BsnI GGCC 3 cut(s) 253, 495, 1172
Bso31I GGTCTC 1 cut(s) 972
Bsp1286I GDGCHC 3 cut(s) 1188, 1234, 1277
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 5 cut(s) 3, 241, 663, 677, 1094
BspACI CCGC 6 cut(s) 362, 496, 498, 1006, 1064, 1355
BspANI GGCC 3 cut(s) 253, 495, 1172
BspCNI CTCAG 4 cut(s) 362, 399, 400, 1316
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 362, 498
BspLI GGNNCC 4 cut(s) 679, 751, 1185, 1231
BspMAI CTGCAG 1 cut(s) 543
BspMI ACCTGC 1 cut(s) 23
BspPI GGATC 4 cut(s) 236, 671, 672, 685
BspT107I GGYRCC 1 cut(s) 1229
BspTNI GGTCTC 1 cut(s) 972
BsrDI GCAATG 1 cut(s) 559
BsrI ACTGG 2 cut(s) 1088, 1094
BssECI CCNNGG 7 cut(s) 85, 496, 899, 997, 1035, 1234, 1261
BssMI GATC 5 cut(s) 3, 241, 663, 677, 1094
BssT1I CCWWGG 3 cut(s) 899, 1035, 1234
Bst2UI CCWGG 5 cut(s) 87, 272, 378, 998, 1262
Bst4CI ACNGT 1 cut(s) 1178
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 3 cut(s) 166, 281, 895
BstBAI YACGTR 2 cut(s) 201, 925
BstC8I GCNNGC 2 cut(s) 1224, 1409
BstDEI CTNAG 4 cut(s) 349, 386, 408, 1303
BstDSI CCRYGG 1 cut(s) 496
BstF5I GGATG 7 cut(s) 94, 533, 561, 730, 918, 1327, 1364
BstFNI CGCG 2 cut(s) 362, 498
BstHHI GCGC 1 cut(s) 1201
BstKTI GATC 5 cut(s) 6, 244, 666, 680, 1097
BstMAI GTCTC 2 cut(s) 972, 1052
BstMBI GATC 5 cut(s) 3, 241, 663, 677, 1094
BstMWI GCNNNNNNNGC 5 cut(s) 166, 281, 448, 895, 1281
BstNI CCWGG 5 cut(s) 87, 272, 378, 998, 1262
BstNSI RCATGY 4 cut(s) 442, 1226, 1330, 1438
BstSCI CCNGG 5 cut(s) 85, 270, 376, 996, 1260
BstSFI CTRYAG 2 cut(s) 539, 1174
BstSLI GKGCMC 1 cut(s) 1234
BstUI CGCG 2 cut(s) 362, 498
BstV1I GCAGC 4 cut(s) 153, 792, 1183, 1350
BstX2I RGATCY 2 cut(s) 663, 677
BstXI CCANNNNNNTGG 1 cut(s) 518
BstYI RGATCY 2 cut(s) 663, 677
BsuRI GGCC 3 cut(s) 253, 495, 1172
BtgI CCRYGG 1 cut(s) 496
BtgZI GCGATG 2 cut(s) 672, 834
BtsCI GGATG 7 cut(s) 94, 533, 561, 730, 918, 1327, 1364
BtsI GCAGTG 5 cut(s) 60, 715, 747, 979, 1011
BtsIMutI CAGTG 6 cut(s) 60, 715, 747, 979, 1011, 1183
BveI ACCTGC 1 cut(s) 23
Cac8I GCNNGC 2 cut(s) 1224, 1409
CaiI CAGNNNCTG 1 cut(s) 15
CfoI GCGC 1 cut(s) 1201
Cfr13I GGNCC 2 cut(s) 750, 1307
Cfr42I CCGCGG 1 cut(s) 499
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 544, 958
CspCI CAANNNNNGTGG 2 cut(s) 1035, 1070
CviAII CATG 8 cut(s) 308, 439, 802, 1219, 1223, 1327, 1404, 1435
CviQI GTAC 2 cut(s) 544, 958
DdeI CTNAG 4 cut(s) 349, 386, 408, 1303
DpnI GATC 5 cut(s) 5, 243, 665, 679, 1096
DpnII GATC 5 cut(s) 3, 241, 663, 677, 1094
DraIII CACNNNGTG 1 cut(s) 571
EaeI YGGCCR 2 cut(s) 251, 493
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 1079
Ecl136II GAGCTC 1 cut(s) 1275
Eco130I CCWWGG 3 cut(s) 899, 1035, 1234
Eco147I AGGCCT 1 cut(s) 1172
Eco24I GRGCYC 2 cut(s) 1188, 1277
Eco31I GGTCTC 1 cut(s) 972
Eco47I GGWCC 2 cut(s) 750, 1307
Eco53kI GAGCTC 1 cut(s) 1275
Eco72I CACGTG 1 cut(s) 925
EcoICRI GAGCTC 1 cut(s) 1275
EcoRII CCWGG 5 cut(s) 85, 270, 376, 996, 1260
EcoT14I CCWWGG 3 cut(s) 899, 1035, 1234
EcoT22I ATGCAT 3 cut(s) 533, 1224, 1440
EcoT38I GRGCYC 2 cut(s) 1188, 1277
ErhI CCWWGG 3 cut(s) 899, 1035, 1234
Esp3I CGTCTC 1 cut(s) 1052
FaeI CATG 8 cut(s) 311, 442, 805, 1222, 1226, 1330, 1407, 1438
FaqI GGGAC 2 cut(s) 1118, 1265
FatI CATG 8 cut(s) 307, 438, 801, 1218, 1222, 1326, 1403, 1434
FauNDI CATATG 1 cut(s) 689
Fnu4HI GCNGC 6 cut(s) 167, 496, 806, 1197, 1339, 1355
FokI GGATG 7 cut(s) 81, 540, 548, 737, 905, 1334, 1351
FriOI GRGCYC 2 cut(s) 1188, 1277
Fsp4HI GCNGC 6 cut(s) 167, 496, 806, 1197, 1339, 1355
FspBI CTAG 1 cut(s) 900
GlaI GCGC 1 cut(s) 1200
GluI GCNGC 6 cut(s) 167, 496, 806, 1197, 1339, 1355
GsuI CTGGAG 1 cut(s) 1111
HaeIII GGCC 3 cut(s) 253, 495, 1172
HapII CCGG 2 cut(s) 210, 245
HgaI GACGC 1 cut(s) 368
HhaI GCGC 1 cut(s) 1201
Hin1II CATG 8 cut(s) 311, 442, 805, 1222, 1226, 1330, 1407, 1438
Hin6I GCGC 1 cut(s) 1199
HinP1I GCGC 1 cut(s) 1199
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HinfI GANTC 6 cut(s) 194, 347, 367, 400, 647, 836
HpaII CCGG 2 cut(s) 210, 245
HphI GGTGA 6 cut(s) 31, 335, 376, 844, 934, 1054
Hpy166II GTNNAC 4 cut(s) 511, 753, 931, 1117
Hpy188I TCNGA 7 cut(s) 352, 372, 389, 405, 652, 968, 1099
Hpy188III TCNNGA 6 cut(s) 191, 245, 418, 840, 1310, 1332
Hpy8I GTNNAC 4 cut(s) 511, 753, 931, 1117
Hpy99I CGWCG 2 cut(s) 362, 1289
HpyAV CCTTC 6 cut(s) 127, 150, 871, 903, 1061, 1162
HpyCH4III ACNGT 1 cut(s) 1178
HpyCH4IV ACGT 2 cut(s) 200, 924
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 281, 448, 895, 1281
HpyF3I CTNAG 4 cut(s) 349, 386, 408, 1303
HpySE526I ACGT 2 cut(s) 200, 924
Hsp92II CATG 8 cut(s) 311, 442, 805, 1222, 1226, 1330, 1407, 1438
HspAI GCGC 1 cut(s) 1199
Kpn2I TCCGGA 1 cut(s) 244
KspI CCGCGG 1 cut(s) 499
Kzo9I GATC 5 cut(s) 3, 241, 663, 677, 1094
LmnI GCTCC 6 cut(s) 500, 1021, 1121, 1153, 1183, 1272
Lsp1109I GCAGC 4 cut(s) 153, 792, 1183, 1350
LweI GCATC 5 cut(s) 103, 518, 540, 727, 1145
MaeI CTAG 1 cut(s) 900
MaeII ACGT 2 cut(s) 200, 924
MaeIII GTNAC 5 cut(s) 337, 364, 812, 850, 1042
MalI GATC 5 cut(s) 5, 243, 665, 679, 1096
MboI GATC 5 cut(s) 3, 241, 663, 677, 1094
MboII GAAGA 3 cut(s) 26, 460, 1372
MflI RGATCY 2 cut(s) 663, 677
MhlI GDGCHC 3 cut(s) 1188, 1234, 1277
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 2 cut(s) 775, 987
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 3 cut(s) 341, 361, 830
Mox20I TGGCCA 1 cut(s) 253
Mph1103I ATGCAT 3 cut(s) 533, 1224, 1440
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 114
MscI TGGCCA 1 cut(s) 253
MseI TTAA 4 cut(s) 126, 138, 990, 1158
MslI CAYNNNNRTG 3 cut(s) 819, 923, 1227
Msp20I TGGCCA 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 2 cut(s) 210, 245
MspR9I CCNGG 5 cut(s) 87, 272, 378, 998, 1262
Mva1269I GAATGC 3 cut(s) 65, 849, 900
MvaI CCWGG 5 cut(s) 87, 272, 378, 998, 1262
MvnI CGCG 2 cut(s) 362, 498
MwoI GCNNNNNNNGC 5 cut(s) 166, 281, 448, 895, 1281
NdeI CATATG 1 cut(s) 689
NdeII GATC 5 cut(s) 3, 241, 663, 677, 1094
NlaIII CATG 8 cut(s) 311, 442, 805, 1222, 1226, 1330, 1407, 1438
NlaIV GGNNCC 4 cut(s) 679, 751, 1185, 1231
NmuCI GTSAC 5 cut(s) 337, 364, 812, 850, 1042
NsiI ATGCAT 3 cut(s) 533, 1224, 1440
NspI RCATGY 4 cut(s) 442, 1226, 1330, 1438
PaeI GCATGC 1 cut(s) 1226
PceI AGGCCT 1 cut(s) 1172
PciI ACATGT 1 cut(s) 1326
PctI GAATGC 3 cut(s) 65, 849, 900
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 3 cut(s) 194, 400, 647
PkrI GCNGC 6 cut(s) 168, 497, 807, 1198, 1340, 1356
PleI GAGTC 3 cut(s) 341, 361, 830
PmaCI CACGTG 1 cut(s) 925
PmlI CACGTG 1 cut(s) 925
PpsI GAGTC 3 cut(s) 341, 361, 830
Ppu21I YACGTR 2 cut(s) 201, 925
PscI ACATGT 1 cut(s) 1326
PsiI TTATAA 1 cut(s) 1444
Psp124BI GAGCTC 1 cut(s) 1277
Psp6I CCWGG 5 cut(s) 85, 270, 376, 996, 1260
PspCI CACGTG 1 cut(s) 925
PspGI CCWGG 5 cut(s) 85, 270, 376, 996, 1260
PspN4I GGNNCC 4 cut(s) 679, 751, 1185, 1231
PspPI GGNCC 2 cut(s) 750, 1307
PstI CTGCAG 1 cut(s) 543
PstNI CAGNNNCTG 1 cut(s) 15
PsuI RGATCY 2 cut(s) 663, 677
RsaI GTAC 2 cut(s) 545, 959
RsaNI GTAC 2 cut(s) 544, 958
RseI CAYNNNNRTG 3 cut(s) 819, 923, 1227
SacI GAGCTC 1 cut(s) 1277
SacII CCGCGG 1 cut(s) 499
SaqAI TTAA 4 cut(s) 126, 138, 990, 1158
SatI GCNGC 6 cut(s) 167, 496, 806, 1197, 1339, 1355
Sau3AI GATC 5 cut(s) 3, 241, 663, 677, 1094
Sau96I GGNCC 2 cut(s) 750, 1307
SchI GAGTC 3 cut(s) 341, 361, 830
ScrFI CCNGG 5 cut(s) 87, 272, 378, 998, 1262
SduI GDGCHC 3 cut(s) 1188, 1234, 1277
SfaNI GCATC 5 cut(s) 103, 518, 540, 727, 1145
SfcI CTRYAG 2 cut(s) 539, 1174
Sfr303I CCGCGG 1 cut(s) 499
SgrBI CCGCGG 1 cut(s) 499
SinI GGWCC 2 cut(s) 750, 1307
SmiMI CAYNNNNRTG 3 cut(s) 819, 923, 1227
SphI GCATGC 1 cut(s) 1226
Sse9I AATT 2 cut(s) 775, 987
SseBI AGGCCT 1 cut(s) 1172
SsiI CCGC 6 cut(s) 362, 496, 498, 1006, 1064, 1355
SspMI CTAG 1 cut(s) 900
SstI GAGCTC 1 cut(s) 1277
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 5 cut(s) 85, 270, 376, 996, 1260
StyI CCWWGG 3 cut(s) 899, 1035, 1234
TaaI ACNGT 1 cut(s) 1178
TaiI ACGT 2 cut(s) 203, 927
TaqI TCGA 2 cut(s) 489, 1277
TasI AATT 2 cut(s) 775, 987
TatI WGTACW 1 cut(s) 543
TauI GCSGC 2 cut(s) 498, 1357
TfiI GAWTC 3 cut(s) 194, 400, 647
Tru1I TTAA 4 cut(s) 126, 138, 990, 1158
Tru9I TTAA 4 cut(s) 126, 138, 990, 1158
TscAI CASTG 6 cut(s) 67, 722, 747, 979, 1018, 1183
TseFI GTSAC 5 cut(s) 337, 364, 812, 850, 1042
TseI GCWGC 4 cut(s) 166, 805, 1196, 1338
Tsp45I GTSAC 5 cut(s) 337, 364, 812, 850, 1042
TspDTI ATGAA 3 cut(s) 123, 1353, 1381
TspGWI ACGGA 5 cut(s) 356, 1008, 1134, 1302, 1334
TspRI CASTG 6 cut(s) 67, 722, 747, 979, 1018, 1183
VpaK11BI GGWCC 2 cut(s) 750, 1307
XceI RCATGY 4 cut(s) 442, 1226, 1330, 1438
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XmaJI CCTAGG 1 cut(s) 899
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 1 cut(s) 900
Zsp2I ATGCAT 3 cut(s) 533, 1224, 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.