pycom04g08490

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007639
Physical Location & Seq
Forward (+)
10190540 .. 10192571
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08490.3

Sequence Viewer

Length: 699 bp
ATGGGGTCCACAGGAATAACAATATCAAACAATTATTTTTCTCATCACAATGAAGTCATGCTGCTGGGCCACAGCGATGACTACTTGCCCGACTCCGGCATGCAAGTCACCATAGCCTTCAATCACTTTGGAGAAAAGTTGGTGCAGAGAATGCCTAGAAGGGGTTACATCCACGTGGTCAACAATGACTTCACTCAATGGGAAATGTACGCCATCGGAGGCAGTGGAAACCCTACAATTAACAGCCAGGGAAACCGTTACATTGCTCCATCAGACCGTAATGCCAAGGAGGTGACAAAGCGTGTGGAAACAGCGGAAGGGAAGTGGAGGGACTGGAACTGGAGATCGGAGGGGGACATAATGGTAAATGGAGCATTTTTTGTGGCATCTGGGGAGGGAATGGAGCTTCAGTATGAGAAGGCCTACAGTGTGGAACCCAAGTCTGCTGTGCTTATTGACCAACTCATCATGCATGCTGGTGCGCTTGGCGTTGGTGGCAGGGACAACAACCTGGGGAAGTGGAGCTCGGACGGCGACGGAAATGGTTTAGGCTCTGGTCCAGACTATACGGATGACATGTCGGGAAGCAGCAGCATTCTACTGCCGCCACTTCCATCCACCTACACTGTCTTCTCCTTTTTCATCTCATTGTCATGCTTGCTAGTTTTGTATATTATCCCAGACGACATGCATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

233

Amino Acids

25.58

Weight (kDa)

4.94

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 697
AciI CCGC 2 cut(s) 314, 605
AcuI CTGAAG 1 cut(s) 392
AcvI CACGTG 1 cut(s) 175
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 2 cut(s) 95, 161
AflIII ACRYGT 1 cut(s) 576
AgsI TTSAA 1 cut(s) 121
AjnI CCWGG 2 cut(s) 246, 510
AluBI AGCT 2 cut(s) 406, 525
AluI AGCT 2 cut(s) 406, 525
Alw21I GWGCWC 1 cut(s) 527
AoxI GGCC 2 cut(s) 67, 420
ApeKI GCWGC 3 cut(s) 61, 588, 591
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 3 cut(s) 6, 67, 557
AsuHPI GGTGA 2 cut(s) 100, 304
AvaII GGWCC 2 cut(s) 6, 557
BanII GRGCYC 1 cut(s) 527
BbrPI CACGTG 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 3 cut(s) 48, 600, 603
BccI CCATC 3 cut(s) 221, 277, 622
BceAI ACGGC 1 cut(s) 547
BciT130I CCWGG 2 cut(s) 248, 512
BfaI CTAG 2 cut(s) 156, 662
BfmI CTRYAG 1 cut(s) 424
BisI GCNGC 4 cut(s) 62, 589, 592, 605
BlsI GCNGC 4 cut(s) 63, 590, 593, 606
Bme1390I CCNGG 2 cut(s) 248, 512
Bme18I GGWCC 2 cut(s) 6, 557
BmgT120I GGNCC 3 cut(s) 6, 67, 557
BmiI GGNNCC 2 cut(s) 7, 435
BmrFI CCNGG 2 cut(s) 248, 512
BmsI GCATC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 622
BpmI CTGGAG 1 cut(s) 361
BsaAI YACGTR 1 cut(s) 175
BsaJI CCNNGG 3 cut(s) 247, 285, 511
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 161
Bse1I ACTGG 2 cut(s) 338, 344
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 248, 512
BseDI CCNNGG 3 cut(s) 247, 285, 511
BseGI GGATG 3 cut(s) 168, 577, 614
BseLI CCNNNNNNNGG 2 cut(s) 95, 161
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 338, 344
BseXI GCAGC 3 cut(s) 48, 600, 603
BseYI CCCAGC 1 cut(s) 64
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 2 cut(s) 69, 422
BsiHKAI GWGCWC 1 cut(s) 527
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 3 cut(s) 344, 368, 515
BslI CCNNNNNNNGG 2 cut(s) 95, 161
BsmFI GGGAC 3 cut(s) 344, 368, 515
BsmI GAATGC 2 cut(s) 156, 594
BsnI GGCC 2 cut(s) 69, 422
Bsp1286I GDGCHC 1 cut(s) 527
Bsp143I GATC 1 cut(s) 344
BspACI CCGC 2 cut(s) 314, 605
BspANI GGCC 2 cut(s) 69, 422
BspLI GGNNCC 2 cut(s) 7, 435
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 338, 344
BssECI CCNNGG 3 cut(s) 247, 285, 511
BssMI GATC 1 cut(s) 344
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 248, 512
Bst4CI ACNGT 4 cut(s) 257, 278, 428, 628
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 3 cut(s) 101, 474, 659
BstF5I GGATG 3 cut(s) 168, 577, 614
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstMWI GCNNNNNNNGC 3 cut(s) 151, 495, 531
BstNI CCWGG 2 cut(s) 248, 512
BstNSI RCATGY 4 cut(s) 103, 476, 580, 691
BstSCI CCNGG 2 cut(s) 246, 510
BstSFI CTRYAG 1 cut(s) 424
BstV1I GCAGC 3 cut(s) 48, 600, 603
BstV2I GAAGAC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 69, 422
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 168, 577, 614
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 3 cut(s) 229, 433, 624
Cac8I GCNNGC 3 cut(s) 101, 474, 659
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 3 cut(s) 6, 67, 557
Csp6I GTAC 1 cut(s) 208
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CviAII CATG 7 cut(s) 58, 100, 469, 473, 577, 654, 688
CviJI RGCY 7 cut(s) 69, 116, 246, 406, 422, 525, 552
CviKI_1 RGCY 7 cut(s) 69, 116, 246, 406, 422, 525, 552
CviQI GTAC 1 cut(s) 208
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 285
Eco147I AGGCCT 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 527
Eco47I GGWCC 2 cut(s) 6, 557
Eco53kI GAGCTC 1 cut(s) 525
Eco57I CTGAAG 1 cut(s) 392
Eco72I CACGTG 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 525
EcoRII CCWGG 2 cut(s) 246, 510
EcoT14I CCWWGG 1 cut(s) 285
EcoT22I ATGCAT 2 cut(s) 474, 693
EcoT38I GRGCYC 1 cut(s) 527
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 7 cut(s) 61, 103, 472, 476, 580, 657, 691
FaqI GGGAC 3 cut(s) 344, 368, 515
FatI CATG 7 cut(s) 57, 99, 468, 472, 576, 653, 687
Fnu4HI GCNGC 4 cut(s) 62, 589, 592, 605
FokI GGATG 3 cut(s) 155, 584, 601
FriOI GRGCYC 1 cut(s) 527
Fsp4HI GCNGC 4 cut(s) 62, 589, 592, 605
FspBI CTAG 2 cut(s) 156, 662
GlaI GCGC 1 cut(s) 483
GluI GCNGC 4 cut(s) 62, 589, 592, 605
GsaI CCCAGC 1 cut(s) 68
GsuI CTGGAG 1 cut(s) 361
HaeIII GGCC 2 cut(s) 69, 422
HapII CCGG 1 cut(s) 96
HhaI GCGC 1 cut(s) 484
Hin1II CATG 7 cut(s) 61, 103, 472, 476, 580, 657, 691
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 1 cut(s) 92
HpaII CCGG 1 cut(s) 96
HphI GGTGA 2 cut(s) 100, 304
Hpy166II GTNNAC 2 cut(s) 9, 181
Hpy188I TCNGA 4 cut(s) 218, 274, 349, 529
Hpy188III TCNNGA 2 cut(s) 560, 582
Hpy8I GTNNAC 2 cut(s) 9, 181
Hpy99I CGWCG 1 cut(s) 539
HpyAV CCTTC 4 cut(s) 127, 153, 311, 412
HpyCH4III ACNGT 4 cut(s) 257, 278, 428, 628
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 103, 145, 472, 691
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 495, 531
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 7 cut(s) 61, 103, 472, 476, 580, 657, 691
HspAI GCGC 1 cut(s) 482
Kzo9I GATC 1 cut(s) 344
LmnI GCTCC 4 cut(s) 271, 371, 403, 522
Lsp1109I GCAGC 3 cut(s) 48, 600, 603
LweI GCATC 1 cut(s) 395
MaeI CTAG 2 cut(s) 156, 662
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 4 cut(s) 106, 164, 257, 292
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 527
MluCI AATT 2 cut(s) 31, 237
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 5 cut(s) 212, 283, 321, 343, 388
Mph1103I ATGCAT 2 cut(s) 474, 693
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 5 cut(s) 48, 75, 173, 477, 652
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 248, 512
Mva1269I GAATGC 2 cut(s) 156, 594
MvaI CCWGG 2 cut(s) 248, 512
MwoI GCNNNNNNNGC 3 cut(s) 151, 495, 531
NdeII GATC 1 cut(s) 344
NlaIII CATG 7 cut(s) 61, 103, 472, 476, 580, 657, 691
NlaIV GGNNCC 2 cut(s) 7, 435
NmuCI GTSAC 2 cut(s) 106, 292
NsiI ATGCAT 2 cut(s) 474, 693
NspI RCATGY 4 cut(s) 103, 476, 580, 691
PaeI GCATGC 2 cut(s) 103, 476
PceI AGGCCT 1 cut(s) 422
PciI ACATGT 1 cut(s) 576
PctI GAATGC 2 cut(s) 156, 594
PkrI GCNGC 4 cut(s) 63, 590, 593, 606
PleI GAGTC 1 cut(s) 86
PmaCI CACGTG 1 cut(s) 175
PmlI CACGTG 1 cut(s) 175
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 175
PscI ACATGT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 697
Psp124BI GAGCTC 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 246, 510
PspCI CACGTG 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 2 cut(s) 246, 510
PspN4I GGNNCC 2 cut(s) 7, 435
PspPI GGNCC 3 cut(s) 6, 67, 557
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 5 cut(s) 48, 75, 173, 477, 652
SacI GAGCTC 1 cut(s) 527
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 4 cut(s) 62, 589, 592, 605
Sau3AI GATC 1 cut(s) 344
Sau96I GGNCC 3 cut(s) 6, 67, 557
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 248, 512
SduI GDGCHC 1 cut(s) 527
SetI ASST 6 cut(s) 177, 294, 408, 513, 527, 623
SfaNI GCATC 1 cut(s) 395
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 2 cut(s) 6, 557
SmiMI CAYNNNNRTG 5 cut(s) 48, 75, 173, 477, 652
SphI GCATGC 2 cut(s) 103, 476
Sse9I AATT 2 cut(s) 31, 237
SseBI AGGCCT 1 cut(s) 422
SsiI CCGC 2 cut(s) 314, 605
SspMI CTAG 2 cut(s) 156, 662
SstI GAGCTC 1 cut(s) 527
StuI AGGCCT 1 cut(s) 422
StyD4I CCNGG 2 cut(s) 246, 510
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 4 cut(s) 257, 278, 428, 628
TaiI ACGT 1 cut(s) 177
TasI AATT 2 cut(s) 31, 237
TauI GCSGC 1 cut(s) 607
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 3 cut(s) 229, 433, 631
TseFI GTSAC 2 cut(s) 106, 292
TseI GCWGC 3 cut(s) 61, 588, 591
Tsp45I GTSAC 2 cut(s) 106, 292
TspDTI ATGAA 2 cut(s) 66, 631
TspGWI ACGGA 2 cut(s) 552, 584
TspRI CASTG 3 cut(s) 229, 433, 631
VpaK11BI GGWCC 2 cut(s) 6, 557
XceI RCATGY 4 cut(s) 103, 476, 580, 691
XspI CTAG 2 cut(s) 156, 662
Zsp2I ATGCAT 2 cut(s) 474, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.