MD04G1089800.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007639
Physical Location & Seq
Forward (+)
15955463 .. 15959171
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1089800.v1.1.491

Sequence Viewer

Length: 1461 bp
ATGATCCCCCGAACCTGCATTGTCCTTCTCTTTCTTCTCACCTCATTTTATCCTCTTGCCTCAGCATTCGTCAACCTCACTCTCCCTGGTCAGCATCCCAACCCAGATGAAGTTGTTCTACAAGTTAATAGGATGGTTAATGCTTCATTAGCAAGAAGGCATATGCTGCAAACAACCCAGAAAGACCAGCTGTTTTCTGCATGCTTAACTGGTAACCCGATAGACGACTGCTGGAAATGTGATCCAGATTGGCCAAACAACAGGCAAAGCCTGGCAGATTGTGCAATAGGGTTTGGCCAATATGCTCATGGAGGCAAAGGTGGTGAGTATTACATTGTGACGGACTCTTCGGATGACGATGCCGTGACTCCGAGGCCAGGCACTCTCAGATACGCTGTGATTCAGACTGAGCCACTCTGGATTGTGTTCCCTGGCAACATGCTCATCAAGCTCTCCCAAGAACTCATATTCAACAGCTACAAAACCCTCGATGGCCGCGGAGCCAATGTCCACATTGTGGGTGGTGGATGCATCACTCTGCAGTACATAAGCAATGTCATCATCCACAATGTGCATATCCACCATTGTCATCAGTCAGGTCAGGCTAATGTGCGTTCAAGCCCTACTCACTATGGCTTTCGCACAGAATCAGACGGTGATGGGATCTCCATCTTTGGATCCAAAGACATATGGATAGACCATTGTAGCCTATCACACTGCAAGGATGGACTGATAGATGCAGTGATGGGGTCCACAGGAATAACAATATCAAACAATTATTTTTCTCATCACAATGAAGTCATGCTGCTGGGCCACAGCGATGACTACTTGCCTGACTCCGGCATGCAAGTCACCATAGCCTTCAATCACTTTGGAGAAAAGTTGGTGCAGAGAATGCCTAGGTGTAGAAGGGGTTACATCCACGTGGTCAACAATGACTTCACTCAATGGGAAATGTACGCCATCGGAGGCAGTGGAAACCCTACAATTAACAGCCAGGGAAATCGCTACACTGCTCCATCAGACCGTAATGCCAAGGAGGTGACAAAGCGTGTGGAGACGGCGGAAGGGAAGTGGAGGGACTGGAACTGGAGATCGGAGGGGGACATAATGGTAAATGGAGCATTTTTTGTGGCATCTGGAGAGGGAATGGAGCTTAAGTATGAGAAGGCCTACAGTGTGGAACCCAAGTCTGCTGTGCTTATTGACCAACTCATCATGCATGCTGGTGCCCTTGGTGTTGGTGGCAGGGACAACAACCTGGGGAAGTGGAGCTCGGACGGCGACGGAAATGGTTTAGGCTCTGGTCCAGACTATACGGATGACATGTCGGGAAGCAGCAGCATTCTACTGCCGCCACTTCCATCCACCTGCACTGTCTTCTCCTTTTTCATCTCATTGTCATTCTTGCTAGTTTTGTATGTTATAACTTATCCCAGACGACATGCATTTATAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

487

Amino Acids

53.73

Weight (kDa)

5.92

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 155 - 355 7.7e-24 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1427, 1454
AarI CACCTGC 1 cut(s) 1379
Acc36I ACCTGC 2 cut(s) 23, 1379
AccB1I GGYRCC 1 cut(s) 1229
AccB7I CCANNNNNTGG 1 cut(s) 517
AccII CGCG 1 cut(s) 498
AciI CCGC 4 cut(s) 496, 498, 1064, 1355
AclWI GGATC 4 cut(s) 236, 671, 672, 685
AcoI YGGCCR 3 cut(s) 251, 295, 493
AcvI CACGTG 1 cut(s) 925
AdeI CACNNNGTG 2 cut(s) 517, 571
AfaI GTAC 2 cut(s) 545, 959
AfiI CCNNNNNNNGG 3 cut(s) 377, 517, 839
AflII CTTAAG 1 cut(s) 1157
AflIII ACRYGT 1 cut(s) 1326
AgsI TTSAA 3 cut(s) 472, 618, 865
AjnI CCWGG 6 cut(s) 85, 270, 376, 430, 996, 1260
AluBI AGCT 5 cut(s) 190, 451, 477, 1156, 1275
AluI AGCT 5 cut(s) 190, 451, 477, 1156, 1275
Alw21I GWGCWC 1 cut(s) 1277
Alw26I GTCTC 1 cut(s) 1052
AlwI GGATC 4 cut(s) 236, 671, 672, 685
AoxI GGCC 6 cut(s) 251, 295, 374, 493, 811, 1170
ApeKI GCWGC 4 cut(s) 166, 805, 1338, 1341
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
Asp700I GAANNNNTTC 1 cut(s) 114
AspA2I CCTAGG 1 cut(s) 899
AspS9I GGNCC 3 cut(s) 750, 811, 1307
AsuHPI GGTGA 5 cut(s) 31, 335, 668, 844, 1054
AvaII GGWCC 2 cut(s) 750, 1307
AvrII CCTAGG 1 cut(s) 899
BaeGI GKGCMC 1 cut(s) 1234
BalI TGGCCA 2 cut(s) 253, 297
BamHI GGATCC 1 cut(s) 677
BanI GGYRCC 1 cut(s) 1229
BanII GRGCYC 1 cut(s) 1277
BarI GAAGNNNNNNTAC 2 cut(s) 102, 134
BbrPI CACGTG 1 cut(s) 925
BbsI GAAGAC 1 cut(s) 1372
Bbv12I GWGCWC 1 cut(s) 1277
BbvCI CCTCAGC 1 cut(s) 61
BbvI GCAGC 4 cut(s) 153, 792, 1350, 1353
BccI CCATC 9 cut(s) 127, 485, 653, 677, 719, 739, 971, 1027, 1372
BceAI ACGGC 3 cut(s) 347, 1077, 1297
BciT130I CCWGG 6 cut(s) 87, 272, 378, 432, 998, 1262
BcoDI GTCTC 1 cut(s) 1052
BfaI CTAG 2 cut(s) 900, 1412
BfmI CTRYAG 2 cut(s) 539, 1174
BfrI CTTAAG 1 cut(s) 1157
BfuAI ACCTGC 2 cut(s) 23, 1379
BisI GCNGC 6 cut(s) 167, 496, 806, 1339, 1342, 1355
BlnI CCTAGG 1 cut(s) 899
BlsI GCNGC 6 cut(s) 168, 497, 807, 1340, 1343, 1356
Bme1390I CCNGG 6 cut(s) 87, 272, 378, 432, 998, 1262
Bme18I GGWCC 2 cut(s) 750, 1307
BmgT120I GGNCC 3 cut(s) 750, 811, 1307
BmiI GGNNCC 5 cut(s) 502, 679, 751, 1185, 1231
BmrFI CCNGG 6 cut(s) 87, 272, 378, 432, 998, 1262
BmsI GCATC 6 cut(s) 103, 349, 518, 540, 727, 1145
BpiI GAAGAC 1 cut(s) 1372
BpmI CTGGAG 2 cut(s) 1111, 1161
Bpu10I CCTNAGC 1 cut(s) 61
BsaAI YACGTR 1 cut(s) 925
BsaBI GATNNNNATC 1 cut(s) 668
BsaJI CCNNGG 9 cut(s) 85, 371, 430, 496, 899, 997, 1035, 1234, 1261
BsaXI ACNNNNNCTCC 4 cut(s) 492, 522, 1145, 1175
Bsc4I CCNNNNNNNGG 3 cut(s) 377, 517, 839
Bse1I ACTGG 3 cut(s) 214, 1088, 1094
Bse3DI GCAATG 1 cut(s) 559
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 6 cut(s) 87, 272, 378, 432, 998, 1262
BseDI CCNNGG 9 cut(s) 85, 371, 430, 496, 899, 997, 1035, 1234, 1261
BseGI GGATG 9 cut(s) 94, 138, 358, 533, 561, 730, 918, 1327, 1364
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 3 cut(s) 377, 517, 839
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 3 cut(s) 75, 399, 400
BseNI ACTGG 3 cut(s) 214, 1088, 1094
BseSI GKGCMC 1 cut(s) 1234
BseXI GCAGC 4 cut(s) 153, 792, 1350, 1353
BseYI CCCAGC 1 cut(s) 808
BsgI GTGCAG 2 cut(s) 908, 1357
Bsh1236I CGCG 1 cut(s) 498
BshFI GGCC 6 cut(s) 253, 297, 376, 495, 813, 1172
BshNI GGYRCC 1 cut(s) 1229
BsiHKAI GWGCWC 1 cut(s) 1277
BsiSI CCGG 1 cut(s) 840
BslFI GGGAC 3 cut(s) 1094, 1118, 1265
BslI CCNNNNNNNGG 3 cut(s) 377, 517, 839
BsmAI GTCTC 1 cut(s) 1052
BsmBI CGTCTC 1 cut(s) 1052
BsmFI GGGAC 3 cut(s) 1094, 1118, 1265
BsmI GAATGC 3 cut(s) 65, 900, 1344
BsnI GGCC 6 cut(s) 253, 297, 376, 495, 813, 1172
Bsp1286I GDGCHC 2 cut(s) 1234, 1277
Bsp143I GATC 5 cut(s) 3, 241, 663, 677, 1094
BspACI CCGC 4 cut(s) 496, 498, 1064, 1355
BspANI GGCC 6 cut(s) 253, 297, 376, 495, 813, 1172
BspCNI CTCAG 3 cut(s) 74, 399, 400
BspFNI CGCG 1 cut(s) 498
BspLI GGNNCC 5 cut(s) 502, 679, 751, 1185, 1231
BspMAI CTGCAG 1 cut(s) 543
BspMI ACCTGC 2 cut(s) 23, 1379
BspPI GGATC 4 cut(s) 236, 671, 672, 685
BspT107I GGYRCC 1 cut(s) 1229
BspTI CTTAAG 1 cut(s) 1157
BsrDI GCAATG 1 cut(s) 559
BsrI ACTGG 3 cut(s) 214, 1088, 1094
BssECI CCNNGG 9 cut(s) 85, 371, 430, 496, 899, 997, 1035, 1234, 1261
BssMI GATC 5 cut(s) 3, 241, 663, 677, 1094
BssT1I CCWWGG 3 cut(s) 899, 1035, 1234
Bst2UI CCWGG 6 cut(s) 87, 272, 378, 432, 998, 1262
Bst4CI ACNGT 4 cut(s) 656, 1028, 1178, 1378
Bst6I CTCTTC 1 cut(s) 352
BstAFI CTTAAG 1 cut(s) 1157
BstAPI GCANNNNNTGC 3 cut(s) 166, 281, 895
BstBAI YACGTR 1 cut(s) 925
BstC8I GCNNGC 3 cut(s) 202, 845, 1224
BstDEI CTNAG 3 cut(s) 61, 386, 408
BstDSI CCRYGG 1 cut(s) 496
BstEII GGTNACC 1 cut(s) 212
BstF5I GGATG 9 cut(s) 94, 138, 358, 533, 561, 730, 918, 1327, 1364
BstFNI CGCG 1 cut(s) 498
BstKTI GATC 5 cut(s) 6, 244, 666, 680, 1097
BstMAI GTCTC 1 cut(s) 1052
BstMBI GATC 5 cut(s) 3, 241, 663, 677, 1094
BstMWI GCNNNNNNNGC 6 cut(s) 149, 166, 281, 448, 895, 1281
BstNI CCWGG 6 cut(s) 87, 272, 378, 432, 998, 1262
BstNSI RCATGY 6 cut(s) 204, 442, 847, 1226, 1330, 1448
BstPI GGTNACC 1 cut(s) 212
BstSCI CCNGG 6 cut(s) 85, 270, 376, 430, 996, 1260
BstSFI CTRYAG 2 cut(s) 539, 1174
BstSLI GKGCMC 1 cut(s) 1234
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 4 cut(s) 153, 792, 1350, 1353
BstV2I GAAGAC 1 cut(s) 1372
BstX2I RGATCY 2 cut(s) 663, 677
BstYI RGATCY 2 cut(s) 663, 677
BsuRI GGCC 6 cut(s) 253, 297, 376, 495, 813, 1172
BtgI CCRYGG 1 cut(s) 496
BtgZI GCGATG 1 cut(s) 834
BtsCI GGATG 9 cut(s) 94, 138, 358, 533, 561, 730, 918, 1327, 1364
BtsI GCAGTG 4 cut(s) 715, 747, 979, 1011
BtsIMutI CAGTG 6 cut(s) 715, 747, 979, 1011, 1183, 1374
BveI ACCTGC 2 cut(s) 23, 1379
Cac8I GCNNGC 3 cut(s) 202, 845, 1224
Cfr13I GGNCC 3 cut(s) 750, 811, 1307
Cfr42I CCGCGG 1 cut(s) 499
Csp6I GTAC 2 cut(s) 544, 958
CspCI CAANNNNNGTGG 2 cut(s) 1035, 1070
CviAII CATG 9 cut(s) 201, 308, 439, 802, 844, 1219, 1223, 1327, 1445
CviQI GTAC 2 cut(s) 544, 958
DdeI CTNAG 3 cut(s) 61, 386, 408
DpnI GATC 5 cut(s) 5, 243, 665, 679, 1096
DpnII GATC 5 cut(s) 3, 241, 663, 677, 1094
DraIII CACNNNGTG 2 cut(s) 517, 571
EaeI YGGCCR 3 cut(s) 251, 295, 493
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
EciI GGCGGA 1 cut(s) 1079
Ecl136II GAGCTC 1 cut(s) 1275
Eco130I CCWWGG 3 cut(s) 899, 1035, 1234
Eco147I AGGCCT 1 cut(s) 1172
Eco24I GRGCYC 1 cut(s) 1277
Eco47I GGWCC 2 cut(s) 750, 1307
Eco53kI GAGCTC 1 cut(s) 1275
Eco72I CACGTG 1 cut(s) 925
Eco91I GGTNACC 1 cut(s) 212
EcoICRI GAGCTC 1 cut(s) 1275
EcoO65I GGTNACC 1 cut(s) 212
EcoRII CCWGG 6 cut(s) 85, 270, 376, 430, 996, 1260
EcoT14I CCWWGG 3 cut(s) 899, 1035, 1234
EcoT22I ATGCAT 3 cut(s) 533, 1224, 1450
EcoT38I GRGCYC 1 cut(s) 1277
ErhI CCWWGG 3 cut(s) 899, 1035, 1234
Esp3I CGTCTC 1 cut(s) 1052
FaeI CATG 9 cut(s) 204, 311, 442, 805, 847, 1222, 1226, 1330, 1448
FaqI GGGAC 3 cut(s) 1094, 1118, 1265
FatI CATG 9 cut(s) 200, 307, 438, 801, 843, 1218, 1222, 1326, 1444
FauNDI CATATG 2 cut(s) 162, 689
Fnu4HI GCNGC 6 cut(s) 167, 496, 806, 1339, 1342, 1355
FokI GGATG 9 cut(s) 81, 145, 365, 540, 548, 737, 905, 1334, 1351
FriOI GRGCYC 1 cut(s) 1277
Fsp4HI GCNGC 6 cut(s) 167, 496, 806, 1339, 1342, 1355
FspBI CTAG 2 cut(s) 900, 1412
GluI GCNGC 6 cut(s) 167, 496, 806, 1339, 1342, 1355
GsaI CCCAGC 1 cut(s) 812
GsuI CTGGAG 2 cut(s) 1111, 1161
HaeIII GGCC 6 cut(s) 253, 297, 376, 495, 813, 1172
HapII CCGG 1 cut(s) 840
Hin1II CATG 9 cut(s) 204, 311, 442, 805, 847, 1222, 1226, 1330, 1448
HincII GTYRAC 2 cut(s) 73, 931
HindII GTYRAC 2 cut(s) 73, 931
HinfI GANTC 5 cut(s) 344, 367, 400, 647, 836
HpaII CCGG 1 cut(s) 840
HphI GGTGA 5 cut(s) 31, 335, 668, 844, 1054
Hpy166II GTNNAC 4 cut(s) 73, 511, 753, 931
Hpy188I TCNGA 9 cut(s) 352, 372, 389, 405, 652, 968, 1024, 1099, 1279
Hpy188III TCNNGA 5 cut(s) 245, 418, 1140, 1310, 1332
Hpy8I GTNNAC 4 cut(s) 73, 511, 753, 931
Hpy99I CGWCG 1 cut(s) 1289
HpyAV CCTTC 6 cut(s) 35, 150, 871, 903, 1061, 1162
HpyCH4III ACNGT 4 cut(s) 656, 1028, 1178, 1378
HpyCH4IV ACGT 1 cut(s) 924
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 166, 281, 448, 895, 1281
HpyF3I CTNAG 3 cut(s) 61, 386, 408
HpySE526I ACGT 1 cut(s) 924
Hsp92II CATG 9 cut(s) 204, 311, 442, 805, 847, 1222, 1226, 1330, 1448
KspI CCGCGG 1 cut(s) 499
Kzo9I GATC 5 cut(s) 3, 241, 663, 677, 1094
LmnI GCTCC 5 cut(s) 500, 1021, 1121, 1153, 1272
Lsp1109I GCAGC 4 cut(s) 153, 792, 1350, 1353
LweI GCATC 6 cut(s) 103, 349, 518, 540, 727, 1145
MaeI CTAG 2 cut(s) 900, 1412
MaeII ACGT 1 cut(s) 924
MaeIII GTNAC 6 cut(s) 212, 337, 364, 850, 914, 1042
MalI GATC 5 cut(s) 5, 243, 665, 679, 1096
MboI GATC 5 cut(s) 3, 241, 663, 677, 1094
MboII GAAGA 3 cut(s) 26, 339, 1372
MflI RGATCY 2 cut(s) 663, 677
MhlI GDGCHC 2 cut(s) 1234, 1277
MlsI TGGCCA 2 cut(s) 253, 297
MluCI AATT 2 cut(s) 775, 987
MluNI TGGCCA 2 cut(s) 253, 297
MlyI GAGTC 3 cut(s) 338, 361, 830
Mox20I TGGCCA 2 cut(s) 253, 297
Mph1103I ATGCAT 3 cut(s) 533, 1224, 1450
MroXI GAANNNNTTC 1 cut(s) 114
MscI TGGCCA 2 cut(s) 253, 297
MseI TTAA 5 cut(s) 126, 138, 206, 990, 1158
MslI CAYNNNNRTG 4 cut(s) 792, 819, 923, 1227
Msp20I TGGCCA 2 cut(s) 253, 297
MspA1I CMGCKG 2 cut(s) 190, 498
MspCI CTTAAG 1 cut(s) 1157
MspI CCGG 1 cut(s) 840
MspR9I CCNGG 6 cut(s) 87, 272, 378, 432, 998, 1262
Mva1269I GAATGC 3 cut(s) 65, 900, 1344
MvaI CCWGG 6 cut(s) 87, 272, 378, 432, 998, 1262
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 6 cut(s) 149, 166, 281, 448, 895, 1281
NdeI CATATG 2 cut(s) 162, 689
NdeII GATC 5 cut(s) 3, 241, 663, 677, 1094
NlaIII CATG 9 cut(s) 204, 311, 442, 805, 847, 1222, 1226, 1330, 1448
NlaIV GGNNCC 5 cut(s) 502, 679, 751, 1185, 1231
NmuCI GTSAC 4 cut(s) 337, 364, 850, 1042
NsiI ATGCAT 3 cut(s) 533, 1224, 1450
NspI RCATGY 6 cut(s) 204, 442, 847, 1226, 1330, 1448
PaeI GCATGC 3 cut(s) 204, 847, 1226
PaqCI CACCTGC 1 cut(s) 1379
PceI AGGCCT 1 cut(s) 1172
PciI ACATGT 1 cut(s) 1326
PctI GAATGC 3 cut(s) 65, 900, 1344
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 2 cut(s) 400, 647
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 6 cut(s) 168, 497, 807, 1340, 1343, 1356
PleI GAGTC 3 cut(s) 338, 361, 830
PmaCI CACGTG 1 cut(s) 925
PmlI CACGTG 1 cut(s) 925
PpsI GAGTC 3 cut(s) 338, 361, 830
Ppu21I YACGTR 1 cut(s) 925
PscI ACATGT 1 cut(s) 1326
PsiI TTATAA 2 cut(s) 1427, 1454
Psp124BI GAGCTC 1 cut(s) 1277
Psp6I CCWGG 6 cut(s) 85, 270, 376, 430, 996, 1260
PspCI CACGTG 1 cut(s) 925
PspEI GGTNACC 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 808
PspGI CCWGG 6 cut(s) 85, 270, 376, 430, 996, 1260
PspN4I GGNNCC 5 cut(s) 502, 679, 751, 1185, 1231
PspPI GGNCC 3 cut(s) 750, 811, 1307
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 2 cut(s) 663, 677
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 2 cut(s) 545, 959
RsaNI GTAC 2 cut(s) 544, 958
RseI CAYNNNNRTG 4 cut(s) 792, 819, 923, 1227
SacI GAGCTC 1 cut(s) 1277
SacII CCGCGG 1 cut(s) 499
SaqAI TTAA 5 cut(s) 126, 138, 206, 990, 1158
SatI GCNGC 6 cut(s) 167, 496, 806, 1339, 1342, 1355
Sau3AI GATC 5 cut(s) 3, 241, 663, 677, 1094
Sau96I GGNCC 3 cut(s) 750, 811, 1307
SchI GAGTC 3 cut(s) 338, 361, 830
ScrFI CCNGG 6 cut(s) 87, 272, 378, 432, 998, 1262
SduI GDGCHC 2 cut(s) 1234, 1277
SfaNI GCATC 6 cut(s) 103, 349, 518, 540, 727, 1145
SfcI CTRYAG 2 cut(s) 539, 1174
Sfr303I CCGCGG 1 cut(s) 499
SgrBI CCGCGG 1 cut(s) 499
SinI GGWCC 2 cut(s) 750, 1307
SmiMI CAYNNNNRTG 4 cut(s) 792, 819, 923, 1227
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
SphI GCATGC 3 cut(s) 204, 847, 1226
Sse9I AATT 2 cut(s) 775, 987
SseBI AGGCCT 1 cut(s) 1172
SsiI CCGC 4 cut(s) 496, 498, 1064, 1355
SspMI CTAG 2 cut(s) 900, 1412
SstI GAGCTC 1 cut(s) 1277
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 6 cut(s) 85, 270, 376, 430, 996, 1260
StyI CCWWGG 3 cut(s) 899, 1035, 1234
TaaI ACNGT 4 cut(s) 656, 1028, 1178, 1378
TaiI ACGT 1 cut(s) 927
TaqI TCGA 1 cut(s) 489
TasI AATT 2 cut(s) 775, 987
TatI WGTACW 1 cut(s) 543
TauI GCSGC 2 cut(s) 498, 1357
TfiI GAWTC 2 cut(s) 400, 647
Tru1I TTAA 5 cut(s) 126, 138, 206, 990, 1158
Tru9I TTAA 5 cut(s) 126, 138, 206, 990, 1158
TscAI CASTG 6 cut(s) 722, 747, 979, 1018, 1183, 1381
TseFI GTSAC 4 cut(s) 337, 364, 850, 1042
TseI GCWGC 4 cut(s) 166, 805, 1338, 1341
Tsp45I GTSAC 4 cut(s) 337, 364, 850, 1042
TspDTI ATGAA 4 cut(s) 123, 135, 810, 1381
TspGWI ACGGA 3 cut(s) 356, 1302, 1334
TspRI CASTG 6 cut(s) 722, 747, 979, 1018, 1183, 1381
Van91I CCANNNNNTGG 1 cut(s) 517
Vha464I CTTAAG 1 cut(s) 1157
VpaK11BI GGWCC 2 cut(s) 750, 1307
XceI RCATGY 6 cut(s) 204, 442, 847, 1226, 1330, 1448
XcmI CCANNNNNNNNNTGG 2 cut(s) 305, 518
XmaJI CCTAGG 1 cut(s) 899
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 2 cut(s) 900, 1412
Zsp2I ATGCAT 3 cut(s) 533, 1224, 1450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.