Rroxscaffold_6G00410670

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007639
Physical Location & Seq
Forward (+)
33075237 .. 33079111
3875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410670.1

Sequence Viewer

Length: 1320 bp
ATGCTAAGCGGAACCTGCATTGTCCTCATCTCTCTTCTCGCTTCATTCTCTGTTGCTAAAGCCTACCTCAACCTCACTCTTCCAGGCCAACATCCCAACCCAGATGAAGTTGTTCAAGAAGTTCATAGGAGGGTAAATGCTTCTATAGTAAGAAGACAAATGCTGCAAGTAACCAAGAGAGACCAGTTCTCTTCATGCCTAACCGGAAACCCTATCGACGATTGCTGGAAATGCGACCCAGACTGGCCCAGCAACCGTCAACGCTTAGCAGACTGCGCAATCGGTTTTGGTCAGTATGCTCTTGGAGGCAAAGGTGGGGAGTACTACATTGTCACCGACTCTTCCGACGACGATGCCGTGAATCCAAAGCCGGGAACTCTGAGATACGCTGTGATTCAGCCAGAACCGCTGTGGATTGTGTTCCCGAGCAACATGCTCATCAAGCTTTCTCAGGAGCTCATCTTTAATAGCTACAAGACTCTTGATGGGAGAGGAGCTAATGTTCACATTACGGGTGGGGGTTGCATTACTTTGCAGTATATAAGCAATGTCATCATCCACAATGTGCATATCCACCATTGTGTTCCCTCAGGGAACACTAATGTGCGGTCAAGCCCAACCCACTACGGCTACCGCACGAAGTCGGACGGCGACGGCATCTCCATCTTCGGGTCCAAAGACATATGGGTCGACCACTGCAGCCTATCACGCTGCAAGGACGGTCTGATAGACGCCGTGATGGGTTCCACTGGCATCACAATCTCAAACAACTACTTCTCCAATCACAACGAAGTGATGCTACTGGGCCACAGCGACGATTACGTACCCGACACCGGCATGCAAGTGACCATAGCCTTCAACCACTTCGGCGAGAATCTGGTGCAGCGCATGCCCAGGTGCAGACGCGGCTACATCCACGTGGTGAACAACGACTTCACTGCATGGGAGATGTACGCGATCGGCGGGAGCGGGAACCCCACGATAAATAGCCAGGGCAACCGGTACACGGCGCCGACCAATAGGAACGCGAAGGAGGTGACGAAGCGCGTGGACACGGAGGAGGGGAGTTGGAAGGGGTGGAATTGGAGGTCGGAGGGGGACATAATGGTGAATGGAGCCTTCTTTGTGGCGTCCGGCGCCGGAGTGGAGATCAAGTACGAGAAGGCCTATAGTGTGGAGCCCAAGTCAGCGGTGCTCATTGACCAACTGATCATGCATGCTGGTGCTCTCGGAGTCGGTGGCAGGTTAGGCACAATCCGAGGAAAGAAGTCGGTGATTATTGGCTCGTTTATCAACAATTTAAGCTTTGCAGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

439

Amino Acids

48.25

Weight (kDa)

7.21

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 153 - 408 4.8e-25 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
Acc16I TGCGCA 1 cut(s) 277
Acc36I ACCTGC 2 cut(s) 23, 1233
AccB1I GGYRCC 2 cut(s) 1009, 1136
AccBSI CCGCTC 1 cut(s) 969
AccI GTMKAC 1 cut(s) 690
AccII CGCG 4 cut(s) 906, 956, 1028, 1047
AciI CCGC 8 cut(s) 9, 407, 607, 634, 906, 963, 969, 1190
AcvI CACGTG 1 cut(s) 919
AcyI GRCGYC 4 cut(s) 732, 1010, 1130, 1137
AdeI CACNNNGTG 2 cut(s) 565, 922
AfaI GTAC 5 cut(s) 323, 825, 953, 1004, 1157
AfiI CCNNNNNNNGG 4 cut(s) 371, 669, 833, 1006
AgeI ACCGGT 1 cut(s) 999
AgsI TTSAA 2 cut(s) 116, 859
AjnI CCWGG 3 cut(s) 82, 893, 990
AleI CACNNNNGTG 2 cut(s) 579, 602
AloI GAACNNNNNNTCC 2 cut(s) 486, 518
AluBI AGCT 5 cut(s) 445, 457, 471, 497, 1305
AluI AGCT 5 cut(s) 445, 457, 471, 497, 1305
Alw21I GWGCWC 3 cut(s) 459, 1197, 1228
Alw26I GTCTC 1 cut(s) 174
Ama87I CYCGRG 1 cut(s) 424
AoxI GGCC 4 cut(s) 85, 245, 805, 1164
ApeKI GCWGC 4 cut(s) 163, 699, 711, 883
AsiGI ACCGGT 1 cut(s) 999
Asp700I GAANNNNTTC 1 cut(s) 111
AspLEI GCGC 5 cut(s) 278, 888, 1012, 1047, 1139
AspS9I GGNCC 3 cut(s) 246, 672, 805
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 5 cut(s) 325, 934, 1048, 1120, 1285
AvaI CYCGRG 1 cut(s) 424
AvaII GGWCC 1 cut(s) 672
AxyI CCTNAGG 1 cut(s) 589
BanI GGYRCC 2 cut(s) 1009, 1136
BanII GRGCYC 2 cut(s) 459, 1182
BarI GAAGNNNNNNTAC 2 cut(s) 783, 815
BbrPI CACGTG 1 cut(s) 919
BbsI GAAGAC 1 cut(s) 160
Bbv12I GWGCWC 3 cut(s) 459, 1197, 1228
BbvI GCAGC 4 cut(s) 150, 698, 711, 895
BccI CCATC 3 cut(s) 479, 671, 733
BceAI ACGGC 6 cut(s) 341, 643, 664, 670, 719, 1023
BcgI CGANNNNNNTGC 6 cut(s) 335, 369, 415, 449, 920, 954
BciT130I CCWGG 3 cut(s) 84, 895, 992
BclI TGATCA 1 cut(s) 1209
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 174
BfmI CTRYAG 3 cut(s) 144, 697, 1168
BfoI RGCGCY 2 cut(s) 1013, 1140
BfuAI ACCTGC 2 cut(s) 23, 1233
BisI GCNGC 5 cut(s) 164, 700, 712, 884, 907
BlpI GCTNAGC 2 cut(s) 5, 265
BlsI GCNGC 5 cut(s) 165, 701, 713, 885, 908
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 4 cut(s) 84, 372, 895, 992
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 424
BmgT120I GGNCC 3 cut(s) 246, 672, 805
BmiI GGNNCC 8 cut(s) 13, 673, 745, 974, 1011, 1117, 1138, 1179
BmrFI CCNGG 4 cut(s) 84, 372, 895, 992
BmrI ACTGGG 1 cut(s) 812
BmsI GCATC 4 cut(s) 343, 666, 762, 786
BmuI ACTGGG 1 cut(s) 812
BpiI GAAGAC 1 cut(s) 160
Bpu1102I GCTNAGC 2 cut(s) 5, 265
BpuMI CCSGG 1 cut(s) 372
BsaAI YACGTR 2 cut(s) 823, 919
BsaHI GRCGYC 4 cut(s) 732, 1010, 1130, 1137
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 893, 991, 1258
BsaWI WCCGGW 2 cut(s) 203, 999
Bsc4I CCNNNNNNNGG 4 cut(s) 371, 669, 833, 1006
Bse118I RCCGGY 2 cut(s) 833, 999
Bse1I ACTGG 4 cut(s) 184, 248, 754, 807
Bse21I CCTNAGG 1 cut(s) 589
Bse3DI GCAATG 1 cut(s) 553
BseBI CCWGG 3 cut(s) 84, 895, 992
BseDI CCNNGG 3 cut(s) 893, 991, 1258
BseGI GGATG 3 cut(s) 91, 555, 912
BseLI CCNNNNNNNGG 4 cut(s) 371, 669, 833, 1006
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 3 cut(s) 371, 464, 603
BseNI ACTGG 4 cut(s) 184, 248, 754, 807
BseRI GAGGAG 2 cut(s) 507, 1073
BseXI GCAGC 4 cut(s) 150, 698, 711, 895
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 2 cut(s) 902, 919
Bsh1236I CGCG 4 cut(s) 906, 956, 1028, 1047
Bsh1285I CGRYCG 1 cut(s) 960
BshFI GGCC 4 cut(s) 87, 247, 807, 1166
BshNI GGYRCC 2 cut(s) 1009, 1136
BshTI ACCGGT 1 cut(s) 999
BsiEI CGRYCG 1 cut(s) 960
BsiHKAI GWGCWC 3 cut(s) 459, 1197, 1228
BsiHKCI CYCGRG 1 cut(s) 424
BsiSI CCGG 6 cut(s) 204, 371, 834, 1000, 1134, 1140
BslFI GGGAC 1 cut(s) 1112
BslI CCNNNNNNNGG 4 cut(s) 371, 669, 833, 1006
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 1112
BsnI GGCC 4 cut(s) 87, 247, 807, 1166
Bso31I GGTCTC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 424
Bsp1286I GDGCHC 4 cut(s) 459, 1182, 1197, 1228
Bsp143I GATC 3 cut(s) 957, 1149, 1209
Bsp1720I GCTNAGC 2 cut(s) 5, 265
BspACI CCGC 8 cut(s) 9, 407, 607, 634, 906, 963, 969, 1190
BspANI GGCC 4 cut(s) 87, 247, 807, 1166
BspCNI CTCAG 3 cut(s) 372, 463, 602
BspFNI CGCG 4 cut(s) 906, 956, 1028, 1047
BspLI GGNNCC 8 cut(s) 13, 673, 745, 974, 1011, 1117, 1138, 1179
BspMAI CTGCAG 1 cut(s) 701
BspMI ACCTGC 2 cut(s) 23, 1233
BspT107I GGYRCC 2 cut(s) 1009, 1136
BspTNI GGTCTC 1 cut(s) 174
BsrBI CCGCTC 1 cut(s) 969
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 2 cut(s) 833, 999
BsrI ACTGG 4 cut(s) 184, 248, 754, 807
BssAI RCCGGY 2 cut(s) 833, 999
BssECI CCNNGG 3 cut(s) 893, 991, 1258
BssMI GATC 3 cut(s) 957, 1149, 1209
BssNI GRCGYC 4 cut(s) 732, 1010, 1130, 1137
Bst2UI CCWGG 3 cut(s) 84, 895, 992
Bst4CI ACNGT 2 cut(s) 257, 722
Bst6I CTCTTC 4 cut(s) 39, 84, 196, 346
BstACI GRCGYC 4 cut(s) 732, 1010, 1130, 1137
BstAPI GCANNNNNTGC 1 cut(s) 889
BstBAI YACGTR 2 cut(s) 823, 919
BstC8I GCNNGC 3 cut(s) 839, 890, 1218
BstDEI CTNAG 5 cut(s) 5, 265, 380, 450, 589
BstF5I GGATG 3 cut(s) 91, 555, 912
BstFNI CGCG 4 cut(s) 906, 956, 1028, 1047
BstH2I RGCGCY 2 cut(s) 1013, 1140
BstHHI GCGC 5 cut(s) 278, 888, 1012, 1047, 1139
BstKTI GATC 3 cut(s) 960, 1152, 1212
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 3 cut(s) 957, 1149, 1209
BstMCI CGRYCG 1 cut(s) 960
BstNI CCWGG 3 cut(s) 84, 895, 992
BstNSI RCATGY 4 cut(s) 436, 841, 892, 1220
BstSCI CCNGG 4 cut(s) 82, 370, 893, 990
BstSFI CTRYAG 3 cut(s) 144, 697, 1168
BstSNI TACGTA 1 cut(s) 823
BstUI CGCG 4 cut(s) 906, 956, 1028, 1047
BstV1I GCAGC 4 cut(s) 150, 698, 711, 895
BstV2I GAAGAC 1 cut(s) 160
Bsu36I CCTNAGG 1 cut(s) 589
BsuRI GGCC 4 cut(s) 87, 247, 807, 1166
BtsCI GGATG 3 cut(s) 91, 555, 912
BtsI GCAGTG 2 cut(s) 694, 936
BtsIMutI CAGTG 3 cut(s) 694, 747, 936
BveI ACCTGC 2 cut(s) 23, 1233
Cac8I GCNNGC 3 cut(s) 839, 890, 1218
CfoI GCGC 5 cut(s) 278, 888, 1012, 1047, 1139
Cfr10I RCCGGY 2 cut(s) 833, 999
Cfr13I GGNCC 3 cut(s) 246, 672, 805
CseI GACGC 3 cut(s) 740, 912, 1119
Csp6I GTAC 5 cut(s) 322, 824, 952, 1003, 1156
CspAI ACCGGT 1 cut(s) 999
CviAII CATG 7 cut(s) 195, 433, 838, 889, 942, 1213, 1217
CviQI GTAC 5 cut(s) 322, 824, 952, 1003, 1156
DdeI CTNAG 5 cut(s) 5, 265, 380, 450, 589
DinI GGCGCC 2 cut(s) 1011, 1138
DpnI GATC 3 cut(s) 959, 1151, 1211
DpnII GATC 3 cut(s) 957, 1149, 1209
DraIII CACNNNGTG 2 cut(s) 565, 922
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
Eam1104I CTCTTC 4 cut(s) 39, 84, 196, 346
EarI CTCTTC 4 cut(s) 39, 84, 196, 346
Ecl136II GAGCTC 1 cut(s) 457
Eco105I TACGTA 1 cut(s) 823
Eco147I AGGCCT 1 cut(s) 1166
Eco24I GRGCYC 2 cut(s) 459, 1182
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 457
Eco72I CACGTG 1 cut(s) 919
Eco81I CCTNAGG 1 cut(s) 589
Eco88I CYCGRG 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 457
EcoRII CCWGG 3 cut(s) 82, 893, 990
EcoT22I ATGCAT 1 cut(s) 1218
EcoT38I GRGCYC 2 cut(s) 459, 1182
EgeI GGCGCC 2 cut(s) 1011, 1138
EheI GGCGCC 2 cut(s) 1011, 1138
FaeI CATG 7 cut(s) 198, 436, 841, 892, 945, 1216, 1220
FaqI GGGAC 1 cut(s) 1112
FatI CATG 7 cut(s) 194, 432, 837, 888, 941, 1212, 1216
FauI CCCGC 2 cut(s) 956, 962
FauNDI CATATG 1 cut(s) 683
FbaI TGATCA 1 cut(s) 1209
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 5 cut(s) 164, 700, 712, 884, 907
FokI GGATG 3 cut(s) 78, 542, 899
FriOI GRGCYC 2 cut(s) 459, 1182
Fsp4HI GCNGC 5 cut(s) 164, 700, 712, 884, 907
FspI TGCGCA 1 cut(s) 277
GlaI GCGC 5 cut(s) 277, 887, 1011, 1046, 1138
GluI GCNGC 5 cut(s) 164, 700, 712, 884, 907
GsaI CCCAGC 1 cut(s) 252
HaeII RGCGCY 2 cut(s) 1013, 1140
HaeIII GGCC 4 cut(s) 87, 247, 807, 1166
HapII CCGG 6 cut(s) 204, 371, 834, 1000, 1134, 1140
HgaI GACGC 3 cut(s) 740, 912, 1119
HhaI GCGC 5 cut(s) 278, 888, 1012, 1047, 1139
Hin1I GRCGYC 4 cut(s) 732, 1010, 1130, 1137
Hin1II CATG 7 cut(s) 198, 436, 841, 892, 945, 1216, 1220
Hin6I GCGC 5 cut(s) 276, 886, 1010, 1045, 1137
HinP1I GCGC 5 cut(s) 276, 886, 1010, 1045, 1137
HincII GTYRAC 2 cut(s) 260, 691
HindII GTYRAC 2 cut(s) 260, 691
HindIII AAGCTT 2 cut(s) 443, 1303
HinfI GANTC 6 cut(s) 338, 361, 394, 478, 874, 1233
HpaII CCGG 6 cut(s) 204, 371, 834, 1000, 1134, 1140
HphI GGTGA 5 cut(s) 325, 934, 1048, 1120, 1285
Hpy166II GTNNAC 6 cut(s) 260, 505, 691, 925, 1005, 1051
Hpy188I TCNGA 7 cut(s) 346, 381, 646, 726, 1093, 1232, 1259
Hpy188III TCNNGA 4 cut(s) 116, 424, 452, 482
Hpy8I GTNNAC 6 cut(s) 260, 505, 691, 925, 1005, 1051
Hpy99I CGWCG 5 cut(s) 221, 350, 353, 656, 818
HpyAV CCTTC 5 cut(s) 865, 1024, 1066, 1129, 1156
HpyCH4III ACNGT 2 cut(s) 257, 722
HpyCH4IV ACGT 2 cut(s) 822, 918
HpyF3I CTNAG 5 cut(s) 5, 265, 380, 450, 589
HpySE526I ACGT 2 cut(s) 822, 918
Hsp92I GRCGYC 4 cut(s) 732, 1010, 1130, 1137
Hsp92II CATG 7 cut(s) 198, 436, 841, 892, 945, 1216, 1220
HspAI GCGC 5 cut(s) 276, 886, 1010, 1045, 1137
KasI GGCGCC 2 cut(s) 1009, 1136
Ksp22I TGATCA 1 cut(s) 1209
Kzo9I GATC 3 cut(s) 957, 1149, 1209
LmnI GCTCC 5 cut(s) 454, 494, 966, 1115, 1177
Lsp1109I GCAGC 4 cut(s) 150, 698, 711, 895
LweI GCATC 4 cut(s) 343, 666, 762, 786
MaeII ACGT 2 cut(s) 822, 918
MaeIII GTNAC 4 cut(s) 169, 331, 844, 1036
MalI GATC 3 cut(s) 959, 1151, 1211
MbiI CCGCTC 1 cut(s) 969
MboI GATC 3 cut(s) 957, 1149, 1209
MboII GAAGA 6 cut(s) 26, 71, 165, 183, 333, 658
MhlI GDGCHC 4 cut(s) 459, 1182, 1197, 1228
MluCI AATT 2 cut(s) 1081, 1297
Mly113I GGCGCC 2 cut(s) 1010, 1137
MlyI GAGTC 3 cut(s) 332, 472, 1242
MmeI TCCRAC 4 cut(s) 369, 624, 1049, 1071
Mph1103I ATGCAT 1 cut(s) 1218
MroXI GAANNNNTTC 1 cut(s) 111
MseI TTAA 2 cut(s) 465, 1301
MslI CAYNNNNRTG 7 cut(s) 579, 602, 836, 842, 917, 1106, 1221
MspA1I CMGCKG 2 cut(s) 409, 1190
MspI CCGG 6 cut(s) 204, 371, 834, 1000, 1134, 1140
MspR9I CCNGG 4 cut(s) 84, 372, 895, 992
MvaI CCWGG 3 cut(s) 84, 895, 992
MvnI CGCG 4 cut(s) 906, 956, 1028, 1047
NarI GGCGCC 2 cut(s) 1010, 1137
NciI CCSGG 1 cut(s) 372
NdeI CATATG 1 cut(s) 683
NdeII GATC 3 cut(s) 957, 1149, 1209
NlaIII CATG 7 cut(s) 198, 436, 841, 892, 945, 1216, 1220
NlaIV GGNNCC 8 cut(s) 13, 673, 745, 974, 1011, 1117, 1138, 1179
NmuCI GTSAC 3 cut(s) 331, 844, 1036
NsbI TGCGCA 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 1218
NspI RCATGY 4 cut(s) 436, 841, 892, 1220
OliI CACNNNNGTG 2 cut(s) 579, 602
PaeI GCATGC 3 cut(s) 841, 892, 1220
PceI AGGCCT 1 cut(s) 1166
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 111
PfeI GAWTC 3 cut(s) 361, 394, 874
PinAI ACCGGT 1 cut(s) 999
PkrI GCNGC 5 cut(s) 165, 701, 713, 885, 908
Ple19I CGATCG 1 cut(s) 960
PleI GAGTC 3 cut(s) 332, 472, 1241
PluTI GGCGCC 2 cut(s) 1013, 1140
PmaCI CACGTG 1 cut(s) 919
PmlI CACGTG 1 cut(s) 919
PpsI GAGTC 3 cut(s) 332, 472, 1241
Ppu21I YACGTR 2 cut(s) 823, 919
Psp124BI GAGCTC 1 cut(s) 459
Psp6I CCWGG 3 cut(s) 82, 893, 990
PspCI CACGTG 1 cut(s) 919
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 3 cut(s) 82, 893, 990
PspN4I GGNNCC 8 cut(s) 13, 673, 745, 974, 1011, 1117, 1138, 1179
PspPI GGNCC 3 cut(s) 246, 672, 805
PstI CTGCAG 1 cut(s) 701
PvuI CGATCG 1 cut(s) 960
RsaI GTAC 5 cut(s) 323, 825, 953, 1004, 1157
RsaNI GTAC 5 cut(s) 322, 824, 952, 1003, 1156
RseI CAYNNNNRTG 7 cut(s) 579, 602, 836, 842, 917, 1106, 1221
SacI GAGCTC 1 cut(s) 459
SalI GTCGAC 1 cut(s) 689
SaqAI TTAA 2 cut(s) 465, 1301
SatI GCNGC 5 cut(s) 164, 700, 712, 884, 907
Sau3AI GATC 3 cut(s) 957, 1149, 1209
Sau96I GGNCC 3 cut(s) 246, 672, 805
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 3 cut(s) 332, 472, 1242
ScrFI CCNGG 4 cut(s) 84, 372, 895, 992
SduI GDGCHC 4 cut(s) 459, 1182, 1197, 1228
SfaNI GCATC 4 cut(s) 343, 666, 762, 786
SfcI CTRYAG 3 cut(s) 144, 697, 1168
SfoI GGCGCC 2 cut(s) 1011, 1138
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 7 cut(s) 579, 602, 836, 842, 917, 1106, 1221
SnaBI TACGTA 1 cut(s) 823
SphI GCATGC 3 cut(s) 841, 892, 1220
Sse9I AATT 2 cut(s) 1081, 1297
SseBI AGGCCT 1 cut(s) 1166
SsiI CCGC 8 cut(s) 9, 407, 607, 634, 906, 963, 969, 1190
SspDI GGCGCC 2 cut(s) 1009, 1136
SstI GAGCTC 1 cut(s) 459
StuI AGGCCT 1 cut(s) 1166
StyD4I CCNGG 4 cut(s) 82, 370, 893, 990
TaaI ACNGT 2 cut(s) 257, 722
TaiI ACGT 2 cut(s) 825, 921
TaqI TCGA 2 cut(s) 216, 690
TasI AATT 2 cut(s) 1081, 1297
TatI WGTACW 1 cut(s) 321
TauI GCSGC 1 cut(s) 909
TfiI GAWTC 3 cut(s) 361, 394, 874
Tru1I TTAA 2 cut(s) 465, 1301
Tru9I TTAA 2 cut(s) 465, 1301
TscAI CASTG 3 cut(s) 701, 754, 943
TseFI GTSAC 3 cut(s) 331, 844, 1036
TseI GCWGC 4 cut(s) 163, 699, 711, 883
Tsp45I GTSAC 3 cut(s) 331, 844, 1036
TspDTI ATGAA 4 cut(s) 33, 113, 120, 183
TspGWI ACGGA 1 cut(s) 1070
TspRI CASTG 3 cut(s) 701, 754, 943
VpaK11BI GGWCC 1 cut(s) 672
XceI RCATGY 4 cut(s) 436, 841, 892, 1220
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 690
XmnI GAANNNNTTC 1 cut(s) 111
ZrmI AGTACT 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.