Prupe.4G097900_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0007639
Physical Location & Seq
Forward (+)
4973344 .. 4974411
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G097900.1

Sequence Viewer

Length: 540 bp
ATGCATAGCTTTAATTACCTCCTCTGTTTTTTTTTTTTCTTCTGTTTCTCAGCCTTTTGTAGATCTGAGTTATCAATCTCTACTTCTTTTTTCCCTTTATTTTTCAGGAAGGAATGTAACCCAAATTGGCCAAACAACAGGCAGAGCTTAGCAGATTGTGCAATTGGGTTTGGCCAATATGCTCTTGGAGGCAAAGGTGGTGAGTATTATATTGTCACAGACTCTTCTAATGATGATGCTGTGAATCCAAGGCCAGGCACTCTGAGTTATGCTGTGATACAGACTGAGCCTCTCTGGATTGTGTTCCCTGGCAACATGCTCATCAAGCTCTCCCAAGAGCTCATCTTCAACAGCTACAAGACCCTTGATGGCCGTGGAGCCAATGTCCACATTGTGGGTGGTGGCTGCATCACATTGCAGTTCATAAGCAATGTCATCATCCACAATGTGCATATCCACAACTGTTATCCGTCAGGTGGCGCTAATATGCGTTCAAGCCCAACCCACTACGGCTACCGCACAAAATCAGACGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

180

Amino Acids

20.04

Weight (kDa)

7.62

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 394
AciI CCGC 1 cut(s) 517
AcoI YGGCCR 3 cut(s) 128, 172, 370
AdeI CACNNNGTG 2 cut(s) 394, 448
AfiI CCNNNNNNNGG 3 cut(s) 254, 394, 476
AgsI TTSAA 2 cut(s) 349, 495
AjnI CCWGG 2 cut(s) 253, 307
AluBI AGCT 5 cut(s) 9, 147, 328, 340, 354
AluI AGCT 5 cut(s) 9, 147, 328, 340, 354
Alw21I GWGCWC 1 cut(s) 342
AoxI GGCC 4 cut(s) 128, 172, 251, 370
ApeKI GCWGC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 212
BalI TGGCCA 2 cut(s) 130, 174
BanII GRGCYC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 392
BccI CCATC 1 cut(s) 362
BceAI ACGGC 2 cut(s) 357, 526
BciT130I CCWGG 2 cut(s) 255, 309
BfoI RGCGCY 1 cut(s) 483
BglII AGATCT 1 cut(s) 62
BisI GCNGC 1 cut(s) 406
BlpI GCTNAGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 407
Bme1390I CCNGG 2 cut(s) 255, 309
BmiI GGNNCC 1 cut(s) 379
BmrFI CCNGG 2 cut(s) 255, 309
BmsI GCATC 2 cut(s) 226, 417
Bpu1102I GCTNAGC 1 cut(s) 148
BsaJI CCNNGG 3 cut(s) 248, 307, 373
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 394, 476
Bse3DI GCAATG 2 cut(s) 413, 436
BseBI CCWGG 2 cut(s) 255, 309
BseDI CCNNGG 3 cut(s) 248, 307, 373
BseGI GGATG 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 254, 394, 476
BseMI GCAATG 2 cut(s) 413, 436
BseMII CTCAG 4 cut(s) 57, 63, 254, 276
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 1 cut(s) 392
BshFI GGCC 4 cut(s) 130, 174, 253, 372
BsiHKAI GWGCWC 1 cut(s) 342
BslI CCNNNNNNNGG 3 cut(s) 254, 394, 476
BsnI GGCC 4 cut(s) 130, 174, 253, 372
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 1 cut(s) 62
Bsp1720I GCTNAGC 1 cut(s) 148
BspACI CCGC 1 cut(s) 517
BspANI GGCC 4 cut(s) 130, 174, 253, 372
BspCNI CTCAG 4 cut(s) 58, 62, 255, 277
BspLI GGNNCC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 413, 436
BssECI CCNNGG 3 cut(s) 248, 307, 373
BssMI GATC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 255, 309
Bst4CI ACNGT 1 cut(s) 464
Bst6I CTCTTC 1 cut(s) 229
BstAPI GCANNNNNTGC 1 cut(s) 158
BstDEI CTNAG 5 cut(s) 49, 66, 148, 263, 285
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 438
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 1 cut(s) 65
BstMBI GATC 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 158, 325
BstNI CCWGG 2 cut(s) 255, 309
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 253, 307
BstV1I GCAGC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 4 cut(s) 130, 174, 253, 372
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 1 cut(s) 438
CfoI GCGC 1 cut(s) 482
CviAII CATG 1 cut(s) 316
DdeI CTNAG 5 cut(s) 49, 66, 148, 263, 285
DpnI GATC 1 cut(s) 64
DpnII GATC 1 cut(s) 62
DraIII CACNNNGTG 2 cut(s) 394, 448
EaeI YGGCCR 3 cut(s) 128, 172, 370
Eam1104I CTCTTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 253, 307
EcoT14I CCWWGG 1 cut(s) 248
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 248
FaeI CATG 1 cut(s) 319
FaiI YATR 8 cut(s) 6, 180, 210, 270, 317, 425, 453, 488
FatI CATG 1 cut(s) 315
Fnu4HI GCNGC 1 cut(s) 406
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 406
GlaI GCGC 1 cut(s) 481
GluI GCNGC 1 cut(s) 406
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 4 cut(s) 130, 174, 253, 372
HhaI GCGC 1 cut(s) 482
Hin1II CATG 1 cut(s) 319
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HinfI GANTC 2 cut(s) 221, 244
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 3 cut(s) 67, 264, 529
Hpy188III TCNNGA 2 cut(s) 106, 295
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4V TGCA 5 cut(s) 4, 161, 408, 418, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 325
HpyF3I CTNAG 5 cut(s) 49, 66, 148, 263, 285
Hsp92II CATG 1 cut(s) 319
HspAI GCGC 1 cut(s) 480
Kzo9I GATC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 377
LpnPI CCDG 8 cut(s) 91, 124, 240, 267, 280, 294, 321, 459
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 2 cut(s) 226, 417
MaeIII GTNAC 2 cut(s) 116, 214
MalI GATC 1 cut(s) 64
MboI GATC 1 cut(s) 62
MboII GAAGA 3 cut(s) 31, 216, 337
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 2 cut(s) 130, 174
MluCI AATT 3 cut(s) 13, 124, 162
MluNI TGGCCA 2 cut(s) 130, 174
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 4 cut(s) 29, 32, 182, 300
Mox20I TGGCCA 2 cut(s) 130, 174
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 2 cut(s) 130, 174
MseI TTAA 1 cut(s) 12
Msp20I TGGCCA 2 cut(s) 130, 174
MspR9I CCNGG 2 cut(s) 255, 309
MunI CAATTG 1 cut(s) 162
MvaI CCWGG 2 cut(s) 255, 309
MwoI GCNNNNNNNGC 2 cut(s) 158, 325
NdeII GATC 1 cut(s) 62
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 319
PfeI GAWTC 1 cut(s) 244
PflMI CCANNNNNTGG 1 cut(s) 394
PkrI GCNGC 1 cut(s) 407
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 253, 307
PspGI CCWGG 2 cut(s) 253, 307
PspN4I GGNNCC 1 cut(s) 379
PsuI RGATCY 1 cut(s) 62
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 1 cut(s) 406
Sau3AI GATC 1 cut(s) 62
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 2 cut(s) 255, 309
SduI GDGCHC 1 cut(s) 342
SetI ASST 8 cut(s) 11, 21, 149, 199, 330, 342, 356, 478
SfaNI GCATC 2 cut(s) 226, 417
Sse9I AATT 3 cut(s) 13, 124, 162
SsiI CCGC 1 cut(s) 517
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 253, 307
StyI CCWWGG 1 cut(s) 248
TaaI ACNGT 1 cut(s) 464
TasI AATT 3 cut(s) 13, 124, 162
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 1 cut(s) 405
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 1 cut(s) 412
TspGWI ACGGA 1 cut(s) 459
Van91I CCANNNNNTGG 1 cut(s) 394
XceI RCATGY 1 cut(s) 319
XcmI CCANNNNNNNNNTGG 2 cut(s) 182, 395
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.