pycom12g10850

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007639
Physical Location & Seq
Forward (+)
12924743 .. 12926532
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g10850.1

Sequence Viewer

Length: 702 bp
ATGGGGTCCACAGGAATAACAATATCAAACAATTATTTCTCTCATCACAACGAAGTCATGCTGCTGGGCCACAGCGATGACTACTTGCCCGACTCTGGAATGCAAGTCACCATAGCCTTCAATCACTTTGGAGAAGAGTTGGTGCAGAGAATGCCTAGGTGTAGAAGGGGCTACATCCACGTGGTCAACAATGACTTCACCCAATGGGAAATGTACGCCATTGGAGGCAGTGGAGAGCCTACAATTAACAGCCAGGGCAACCGCTACACTGCTCCGTCAAACCCTAACGCCAAGGAGGTGACAAAGCGTGTGGAGACGGGGGAAGGGAAGTGGAAGAACTGGAACTGGAGATCGGAGGGGGACATAATGGTAAATGGAGCATTTTTTGTAGCATCTGGGGAGGGAGTGGAGCTTAACTATGAGAAGGCCTACAGTGTGGAGCCCAAGTCTGCTGCGCTTATTGACCAACTCATCATGCATGCTGGTGCCCTTGGTGTTGGTGGCAGGGACAACAACCTGGGGAAGTGGAGCTCGAACGGCGACGGAAATGGTTTAGGCTCAGGTCCAGACTATACGGATGACATGTCGGGAAGCAGCCGTATTCCACTGCCGCCACTTTCATCCACCTCCACCATCTTCTCATTTTTCATCTCATTGTCATGCTTGCTATTTTTGTATATTATCCCAGACATGCATTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

234

Amino Acids

25.58

Weight (kDa)

5.17

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 2 - 163 3.9e-17 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 700
AccB1I GGYRCC 1 cut(s) 485
AciI CCGC 2 cut(s) 262, 611
AcvI CACGTG 1 cut(s) 181
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 1 cut(s) 121
AjnI CCWGG 2 cut(s) 252, 516
AluBI AGCT 2 cut(s) 412, 531
AluI AGCT 2 cut(s) 412, 531
Alw21I GWGCWC 1 cut(s) 533
Alw26I GTCTC 1 cut(s) 308
AoxI GGCC 2 cut(s) 67, 426
ApeKI GCWGC 3 cut(s) 61, 452, 594
AspA2I CCTAGG 1 cut(s) 155
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 3 cut(s) 6, 67, 563
AsuHPI GGTGA 3 cut(s) 100, 190, 310
AvaII GGWCC 2 cut(s) 6, 563
AvrII CCTAGG 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 444, 533
BbrPI CACGTG 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 533
BbvI GCAGC 3 cut(s) 48, 439, 606
BccI CCATC 1 cut(s) 641
BceAI ACGGC 2 cut(s) 553, 582
BciT130I CCWGG 2 cut(s) 254, 518
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 1 cut(s) 430
BisI GCNGC 4 cut(s) 62, 453, 595, 611
BlnI CCTAGG 1 cut(s) 155
BlsI GCNGC 4 cut(s) 63, 454, 596, 612
Bme1390I CCNGG 2 cut(s) 254, 518
Bme18I GGWCC 2 cut(s) 6, 563
BmgT120I GGNCC 3 cut(s) 6, 67, 563
BmiI GGNNCC 3 cut(s) 7, 441, 487
BmrFI CCNGG 2 cut(s) 254, 518
BmsI GCATC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 367
Bpu10I CCTNAGC 1 cut(s) 559
BsaAI YACGTR 1 cut(s) 181
BsaJI CCNNGG 5 cut(s) 155, 253, 291, 490, 517
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 344, 350
BseBI CCWGG 2 cut(s) 254, 518
BseDI CCNNGG 5 cut(s) 155, 253, 291, 490, 517
BseGI GGATG 3 cut(s) 174, 583, 620
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 2 cut(s) 344, 350
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 3 cut(s) 48, 439, 606
BseYI CCCAGC 1 cut(s) 64
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 2 cut(s) 69, 428
BshNI GGYRCC 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 533
BslFI GGGAC 2 cut(s) 374, 521
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 308
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 374, 521
BsmI GAATGC 2 cut(s) 105, 156
BsnI GGCC 2 cut(s) 69, 428
Bsp1286I GDGCHC 3 cut(s) 444, 490, 533
Bsp143I GATC 1 cut(s) 350
BspACI CCGC 2 cut(s) 262, 611
BspANI GGCC 2 cut(s) 69, 428
BspCNI CTCAG 1 cut(s) 572
BspLI GGNNCC 3 cut(s) 7, 441, 487
BspT107I GGYRCC 1 cut(s) 485
BsrI ACTGG 2 cut(s) 344, 350
BssECI CCNNGG 5 cut(s) 155, 253, 291, 490, 517
BssMI GATC 1 cut(s) 350
BssT1I CCWWGG 3 cut(s) 155, 291, 490
Bst2UI CCWGG 2 cut(s) 254, 518
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 480, 665
BstDEI CTNAG 1 cut(s) 559
BstF5I GGATG 3 cut(s) 174, 583, 620
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 1 cut(s) 350
BstMWI GCNNNNNNNGC 2 cut(s) 151, 537
BstNI CCWGG 2 cut(s) 254, 518
BstNSI RCATGY 3 cut(s) 482, 586, 694
BstSCI CCNGG 2 cut(s) 252, 516
BstSFI CTRYAG 1 cut(s) 430
BstSLI GKGCMC 1 cut(s) 490
BstV1I GCAGC 3 cut(s) 48, 439, 606
BsuRI GGCC 2 cut(s) 69, 428
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 174, 583, 620
BtsI GCAGTG 3 cut(s) 235, 267, 605
BtsIMutI CAGTG 4 cut(s) 235, 267, 439, 605
Cac8I GCNNGC 2 cut(s) 480, 665
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 3 cut(s) 6, 67, 563
Csp6I GTAC 1 cut(s) 214
CspCI CAANNNNNGTGG 2 cut(s) 291, 326
CviAII CATG 6 cut(s) 58, 475, 479, 583, 660, 691
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 559
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 531
Eco130I CCWWGG 3 cut(s) 155, 291, 490
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 2 cut(s) 444, 533
Eco47I GGWCC 2 cut(s) 6, 563
Eco53kI GAGCTC 1 cut(s) 531
Eco72I CACGTG 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 531
EcoRII CCWGG 2 cut(s) 252, 516
EcoT14I CCWWGG 3 cut(s) 155, 291, 490
EcoT22I ATGCAT 2 cut(s) 480, 696
EcoT38I GRGCYC 2 cut(s) 444, 533
ErhI CCWWGG 3 cut(s) 155, 291, 490
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 6 cut(s) 61, 478, 482, 586, 663, 694
FaqI GGGAC 2 cut(s) 374, 521
FatI CATG 6 cut(s) 57, 474, 478, 582, 659, 690
Fnu4HI GCNGC 4 cut(s) 62, 453, 595, 611
FokI GGATG 3 cut(s) 161, 590, 607
FriOI GRGCYC 2 cut(s) 444, 533
Fsp4HI GCNGC 4 cut(s) 62, 453, 595, 611
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 456
GluI GCNGC 4 cut(s) 62, 453, 595, 611
GsaI CCCAGC 1 cut(s) 68
GsuI CTGGAG 1 cut(s) 367
HaeIII GGCC 2 cut(s) 69, 428
HhaI GCGC 1 cut(s) 457
Hin1II CATG 6 cut(s) 61, 478, 482, 586, 663, 694
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 1 cut(s) 92
HphI GGTGA 3 cut(s) 100, 190, 310
Hpy166II GTNNAC 2 cut(s) 9, 187
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 3 cut(s) 96, 566, 588
Hpy8I GTNNAC 2 cut(s) 9, 187
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 4 cut(s) 127, 159, 317, 418
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 4 cut(s) 103, 145, 478, 694
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 537
HpyF3I CTNAG 1 cut(s) 559
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 6 cut(s) 61, 478, 482, 586, 663, 694
HspAI GCGC 1 cut(s) 455
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 5 cut(s) 277, 377, 409, 439, 528
Lsp1109I GCAGC 3 cut(s) 48, 439, 606
LweI GCATC 1 cut(s) 401
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 106, 298
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 3 cut(s) 146, 346, 628
MhlI GDGCHC 3 cut(s) 444, 490, 533
MluCI AATT 2 cut(s) 31, 243
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 5 cut(s) 218, 289, 349, 394, 637
Mph1103I ATGCAT 2 cut(s) 480, 696
MseI TTAA 2 cut(s) 246, 414
MslI CAYNNNNRTG 4 cut(s) 75, 179, 483, 658
MspR9I CCNGG 2 cut(s) 254, 518
Mva1269I GAATGC 2 cut(s) 105, 156
MvaI CCWGG 2 cut(s) 254, 518
MwoI GCNNNNNNNGC 2 cut(s) 151, 537
NdeII GATC 1 cut(s) 350
NlaIII CATG 6 cut(s) 61, 478, 482, 586, 663, 694
NlaIV GGNNCC 3 cut(s) 7, 441, 487
NmuCI GTSAC 2 cut(s) 106, 298
NsiI ATGCAT 2 cut(s) 480, 696
NspI RCATGY 3 cut(s) 482, 586, 694
PaeI GCATGC 1 cut(s) 482
PceI AGGCCT 1 cut(s) 428
PciI ACATGT 1 cut(s) 582
PctI GAATGC 2 cut(s) 105, 156
PkrI GCNGC 4 cut(s) 63, 454, 596, 612
PleI GAGTC 1 cut(s) 86
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 181
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 700
Psp124BI GAGCTC 1 cut(s) 533
Psp6I CCWGG 2 cut(s) 252, 516
PspCI CACGTG 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 2 cut(s) 252, 516
PspN4I GGNNCC 3 cut(s) 7, 441, 487
PspPI GGNCC 3 cut(s) 6, 67, 563
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 4 cut(s) 75, 179, 483, 658
SacI GAGCTC 1 cut(s) 533
SaqAI TTAA 2 cut(s) 246, 414
SatI GCNGC 4 cut(s) 62, 453, 595, 611
Sau3AI GATC 1 cut(s) 350
Sau96I GGNCC 3 cut(s) 6, 67, 563
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 254, 518
SduI GDGCHC 3 cut(s) 444, 490, 533
SetI ASST 8 cut(s) 161, 183, 300, 414, 519, 533, 565, 629
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 1 cut(s) 430
SinI GGWCC 2 cut(s) 6, 563
SmiMI CAYNNNNRTG 4 cut(s) 75, 179, 483, 658
SphI GCATGC 1 cut(s) 482
Sse9I AATT 2 cut(s) 31, 243
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 2 cut(s) 262, 611
SspMI CTAG 1 cut(s) 156
SstI GAGCTC 1 cut(s) 533
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 252, 516
StyI CCWWGG 3 cut(s) 155, 291, 490
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 1 cut(s) 183
TaqI TCGA 1 cut(s) 533
TasI AATT 2 cut(s) 31, 243
TauI GCSGC 1 cut(s) 613
Tru1I TTAA 2 cut(s) 246, 414
Tru9I TTAA 2 cut(s) 246, 414
TscAI CASTG 4 cut(s) 235, 274, 439, 612
TseFI GTSAC 2 cut(s) 106, 298
TseI GCWGC 3 cut(s) 61, 452, 594
Tsp45I GTSAC 2 cut(s) 106, 298
TspDTI ATGAA 2 cut(s) 609, 637
TspGWI ACGGA 3 cut(s) 264, 558, 590
TspRI CASTG 4 cut(s) 235, 274, 439, 612
VpaK11BI GGWCC 2 cut(s) 6, 563
XceI RCATGY 3 cut(s) 482, 586, 694
XmaJI CCTAGG 1 cut(s) 155
XspI CTAG 1 cut(s) 156
Zsp2I ATGCAT 2 cut(s) 480, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.