Rh3DG189400

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0007639
Physical Location & Seq
Forward (+)
16892342 .. 16896066
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG189400.1

Sequence Viewer

Length: 1323 bp
ATGAAGTTGTTCAAGAAGTTCATAGGTACATTATTCACCTCTGTTTCCTCTGTTTCTTTCACCCCTCTCTTTGTAGATCTGAGTACTCTATACTTCATTTTTTCTTGCAGGAGGGTAAATGCTTCTATAGTAAGGAGACAAATGCTGCAAGTAACCAAGAGAGACCAGTTCTCTTCATGCCTAACCGGAAACCCTATCGACGATTGCTGGAAATGCGACCCAGACTGGCCCAGCAACCGTCAACGTTTAGCAGACTGCGCAATCGGGTTTGGTCAGTATGCTCTTGGAGGCAAAGGTGGGGAGTACTACATTGTCACCGACTCTTCTGACGATGATGCCGTGAATCCAAAGCCGGGAACTCTGAGATACGCTGTGATTCAGCCAGAACCGCTGTGGATTGTGTTCCCGAGCAACATGCTCATCAAGCTTTCTCAGGAGCTCATCTTTAATAGCTACAAGACCCTTGATGGGAGAGGAGCTAATGTTCACATTACGGGTGGGGGTTGCATTACTTTGCAGTATATAAGCAATGTCATCATCCACAATGTGCATGTCCACCATTGTGTTCCCTCAGGGAACACTAATGTGCGGTCAAGCCCAACCCACTACGGCTACCGCACGAAGTCGGACGGCGACGGCATCTCCATCTTCGGGTCCAAAGACATATGGGTCGACCACTGCAGCCTATCACACTGCAAGGACGGTCTGATCGACGCCGTGATGGGTTCCACAGGCATCACAATCTCAAACAACTACTTCTCCAATCACAACGAAGTGATGCTACTGGGCCACAGCGACGATTACGTCCCCGACACCGGCATGCAGGTGACCATAGCCTTCAACCACTTCGGCGAGAATCTGGTGCAGCGCATGCCCAGGTGCAGACGCGGCTACATCCACGTGGTGAACAACGACTTCACTGCATGGGAGATGTACGCGATCGGCGGGAGCGGGAACCCCACGATAAATAGCCAGGGCAACCGGTACACGGCACCGACCAATAGGAACGCGAAGGAGGTGACGAAGCGCGTGGACACGGAGGAGGGGAGTTGGAAGGGGTGGAATTGGAGGTCGGAGGGGGACATAATGGTGAATGGAGCCTTCTTCGTGGCGTCCGGCGCCGGAGTGGAGATCAAGTACGAGAAGGCCTATAGTGTGGAGCCCAAGTCAGCGGTGCTCATTGACCAACTCATCATGCATGCTGGTGCTCTCGGAGTTGGTGGCAGGTCCGGTCTCTTTTTCTTTTTAGTTCTTCTTTTTCTTTTCCACTGGTGCTGCACTTTGTTTTTTGCTCTTTTGGTTTCACTTTTTCTTTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

440

Amino Acids

48.96

Weight (kDa)

7.19

Isoelectric Point (pI)

32.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 147 - 402 5.5e-25 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 814
AasI GACNNNNNNGTC 2 cut(s) 668, 803
Acc16I TGCGCA 1 cut(s) 259
Acc36I ACCTGC 2 cut(s) 814, 1215
AccB1I GGYRCC 2 cut(s) 991, 1118
AccBSI CCGCTC 1 cut(s) 951
AccI GTMKAC 1 cut(s) 672
AccII CGCG 4 cut(s) 888, 938, 1010, 1029
AciI CCGC 7 cut(s) 389, 589, 616, 888, 945, 951, 1172
AclI AACGTT 1 cut(s) 244
AcvI CACGTG 1 cut(s) 901
AcyI GRCGYC 3 cut(s) 714, 1112, 1119
AdeI CACNNNGTG 2 cut(s) 547, 904
AfaI GTAC 6 cut(s) 28, 85, 305, 935, 986, 1139
AfiI CCNNNNNNNGG 5 cut(s) 353, 468, 651, 815, 988
AgeI ACCGGT 1 cut(s) 981
AgsI TTSAA 2 cut(s) 13, 841
AjnI CCWGG 2 cut(s) 875, 972
AleI CACNNNNGTG 2 cut(s) 561, 584
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 4 cut(s) 427, 439, 453, 479
AluI AGCT 4 cut(s) 427, 439, 453, 479
Alw21I GWGCWC 3 cut(s) 441, 1179, 1210
Alw26I GTCTC 3 cut(s) 130, 156, 1238
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 3 cut(s) 227, 787, 1146
ApeKI GCWGC 4 cut(s) 145, 681, 865, 1275
AsiGI ACCGGT 1 cut(s) 981
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 4 cut(s) 260, 870, 1029, 1121
AspS9I GGNCC 4 cut(s) 228, 654, 787, 1227
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 7 cut(s) 28, 52, 307, 838, 916, 1030, 1102
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 2 cut(s) 654, 1227
AxyI CCTNAGG 1 cut(s) 571
BanI GGYRCC 2 cut(s) 991, 1118
BanII GRGCYC 2 cut(s) 441, 1164
BarI GAAGNNNNNNTAC 2 cut(s) 765, 797
BbrPI CACGTG 1 cut(s) 901
Bbv12I GWGCWC 3 cut(s) 441, 1179, 1210
BbvI GCAGC 4 cut(s) 132, 693, 877, 1262
BccI CCATC 3 cut(s) 461, 653, 715
BceAI ACGGC 6 cut(s) 323, 625, 646, 652, 701, 1005
BcgI CGANNNNNNTGC 4 cut(s) 397, 431, 902, 936
BciT130I CCWGG 2 cut(s) 877, 974
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 3 cut(s) 130, 156, 1238
BfmI CTRYAG 3 cut(s) 126, 679, 1150
BfoI RGCGCY 1 cut(s) 1122
BfuAI ACCTGC 2 cut(s) 814, 1215
BglII AGATCT 1 cut(s) 76
BisI GCNGC 5 cut(s) 146, 682, 866, 889, 1276
BlsI GCNGC 5 cut(s) 147, 683, 867, 890, 1277
BmcAI AGTACT 2 cut(s) 85, 305
Bme1390I CCNGG 3 cut(s) 354, 877, 974
Bme18I GGWCC 2 cut(s) 654, 1227
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 4 cut(s) 228, 654, 787, 1227
BmiI GGNNCC 7 cut(s) 655, 727, 956, 993, 1099, 1120, 1161
BmrFI CCNGG 3 cut(s) 354, 877, 974
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 4 cut(s) 325, 648, 744, 768
BmuI ACTGGG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 901
BsaHI GRCGYC 3 cut(s) 714, 1112, 1119
BsaI GGTCTC 2 cut(s) 156, 1238
BsaJI CCNNGG 2 cut(s) 875, 973
BsaWI WCCGGW 3 cut(s) 185, 981, 1229
Bsc4I CCNNNNNNNGG 5 cut(s) 353, 468, 651, 815, 988
Bse118I RCCGGY 2 cut(s) 815, 981
Bse1I ACTGG 4 cut(s) 166, 230, 789, 1274
Bse21I CCTNAGG 1 cut(s) 571
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 2 cut(s) 877, 974
BseDI CCNNGG 2 cut(s) 875, 973
BseGI GGATG 2 cut(s) 537, 894
BseLI CCNNNNNNNGG 5 cut(s) 353, 468, 651, 815, 988
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 4 cut(s) 71, 353, 446, 585
BseNI ACTGG 4 cut(s) 166, 230, 789, 1274
BseRI GAGGAG 2 cut(s) 489, 1055
BseXI GCAGC 4 cut(s) 132, 693, 877, 1262
BseYI CCCAGC 1 cut(s) 230
BsgI GTGCAG 3 cut(s) 884, 901, 1261
Bsh1236I CGCG 4 cut(s) 888, 938, 1010, 1029
Bsh1285I CGRYCG 1 cut(s) 942
BshFI GGCC 3 cut(s) 229, 789, 1148
BshNI GGYRCC 2 cut(s) 991, 1118
BshTI ACCGGT 1 cut(s) 981
BsiEI CGRYCG 1 cut(s) 942
BsiHKAI GWGCWC 3 cut(s) 441, 1179, 1210
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 7 cut(s) 186, 353, 816, 982, 1116, 1122, 1230
BslFI GGGAC 2 cut(s) 791, 1094
BslI CCNNNNNNNGG 5 cut(s) 353, 468, 651, 815, 988
BsmAI GTCTC 3 cut(s) 130, 156, 1238
BsmFI GGGAC 2 cut(s) 791, 1094
BsnI GGCC 3 cut(s) 229, 789, 1148
Bso31I GGTCTC 2 cut(s) 156, 1238
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 4 cut(s) 441, 1164, 1179, 1210
Bsp143I GATC 4 cut(s) 76, 708, 939, 1131
BspACI CCGC 7 cut(s) 389, 589, 616, 888, 945, 951, 1172
BspANI GGCC 3 cut(s) 229, 789, 1148
BspCNI CTCAG 4 cut(s) 72, 354, 445, 584
BspFNI CGCG 4 cut(s) 888, 938, 1010, 1029
BspLI GGNNCC 7 cut(s) 655, 727, 956, 993, 1099, 1120, 1161
BspMAI CTGCAG 1 cut(s) 683
BspMI ACCTGC 2 cut(s) 814, 1215
BspT107I GGYRCC 2 cut(s) 991, 1118
BspTNI GGTCTC 2 cut(s) 156, 1238
BsrBI CCGCTC 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 535
BsrFI RCCGGY 2 cut(s) 815, 981
BsrI ACTGG 4 cut(s) 166, 230, 789, 1274
BssAI RCCGGY 2 cut(s) 815, 981
BssECI CCNNGG 2 cut(s) 875, 973
BssMI GATC 4 cut(s) 76, 708, 939, 1131
BssNI GRCGYC 3 cut(s) 714, 1112, 1119
Bst2UI CCWGG 2 cut(s) 877, 974
Bst4CI ACNGT 2 cut(s) 239, 704
Bst6I CTCTTC 2 cut(s) 178, 328
BstACI GRCGYC 3 cut(s) 714, 1112, 1119
BstAPI GCANNNNNTGC 1 cut(s) 871
BstBAI YACGTR 1 cut(s) 901
BstC8I GCNNGC 3 cut(s) 821, 872, 1200
BstDEI CTNAG 4 cut(s) 80, 362, 432, 571
BstEII GGTNACC 1 cut(s) 826
BstF5I GGATG 2 cut(s) 537, 894
BstFNI CGCG 4 cut(s) 888, 938, 1010, 1029
BstH2I RGCGCY 1 cut(s) 1122
BstHHI GCGC 4 cut(s) 260, 870, 1029, 1121
BstKTI GATC 4 cut(s) 79, 711, 942, 1134
BstMAI GTCTC 3 cut(s) 130, 156, 1238
BstMBI GATC 4 cut(s) 76, 708, 939, 1131
BstMCI CGRYCG 1 cut(s) 942
BstMWI GCNNNNNNNGC 7 cut(s) 213, 257, 388, 424, 871, 888, 1118
BstNI CCWGG 2 cut(s) 877, 974
BstNSI RCATGY 5 cut(s) 418, 554, 823, 874, 1202
BstPI GGTNACC 1 cut(s) 826
BstSCI CCNGG 3 cut(s) 352, 875, 972
BstSFI CTRYAG 3 cut(s) 126, 679, 1150
BstUI CGCG 4 cut(s) 888, 938, 1010, 1029
BstV1I GCAGC 4 cut(s) 132, 693, 877, 1262
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
Bsu36I CCTNAGG 1 cut(s) 571
BsuRI GGCC 3 cut(s) 229, 789, 1148
BtsCI GGATG 2 cut(s) 537, 894
BtsI GCAGTG 3 cut(s) 676, 691, 918
BtsIMutI CAGTG 4 cut(s) 676, 691, 918, 1267
BveI ACCTGC 2 cut(s) 814, 1215
Cac8I GCNNGC 3 cut(s) 821, 872, 1200
CfoI GCGC 4 cut(s) 260, 870, 1029, 1121
Cfr10I RCCGGY 2 cut(s) 815, 981
Cfr13I GGNCC 4 cut(s) 228, 654, 787, 1227
CseI GACGC 3 cut(s) 722, 894, 1101
Csp6I GTAC 6 cut(s) 27, 84, 304, 934, 985, 1138
CspAI ACCGGT 1 cut(s) 981
CviAII CATG 8 cut(s) 177, 415, 551, 820, 871, 924, 1195, 1199
CviQI GTAC 6 cut(s) 27, 84, 304, 934, 985, 1138
DdeI CTNAG 4 cut(s) 80, 362, 432, 571
DinI GGCGCC 1 cut(s) 1120
DpnI GATC 4 cut(s) 78, 710, 941, 1133
DpnII GATC 4 cut(s) 76, 708, 939, 1131
DraIII CACNNNGTG 2 cut(s) 547, 904
DrdI GACNNNNNNGTC 2 cut(s) 668, 803
DseDI GACNNNNNNGTC 2 cut(s) 668, 803
Eam1104I CTCTTC 2 cut(s) 178, 328
EarI CTCTTC 2 cut(s) 178, 328
Ecl136II GAGCTC 1 cut(s) 439
Eco147I AGGCCT 1 cut(s) 1148
Eco24I GRGCYC 2 cut(s) 441, 1164
Eco31I GGTCTC 2 cut(s) 156, 1238
Eco47I GGWCC 2 cut(s) 654, 1227
Eco53kI GAGCTC 1 cut(s) 439
Eco72I CACGTG 1 cut(s) 901
Eco81I CCTNAGG 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 406
Eco91I GGTNACC 1 cut(s) 826
EcoICRI GAGCTC 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 826
EcoRII CCWGG 2 cut(s) 875, 972
EcoT22I ATGCAT 1 cut(s) 1200
EcoT38I GRGCYC 2 cut(s) 441, 1164
EgeI GGCGCC 1 cut(s) 1120
EheI GGCGCC 1 cut(s) 1120
FaeI CATG 8 cut(s) 180, 418, 554, 823, 874, 927, 1198, 1202
FaqI GGGAC 2 cut(s) 791, 1094
FatI CATG 8 cut(s) 176, 414, 550, 819, 870, 923, 1194, 1198
FauI CCCGC 2 cut(s) 938, 944
FauNDI CATATG 1 cut(s) 665
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 5 cut(s) 146, 682, 866, 889, 1276
FokI GGATG 2 cut(s) 524, 881
FriOI GRGCYC 2 cut(s) 441, 1164
Fsp4HI GCNGC 5 cut(s) 146, 682, 866, 889, 1276
FspI TGCGCA 1 cut(s) 259
GlaI GCGC 4 cut(s) 259, 869, 1028, 1120
GluI GCNGC 5 cut(s) 146, 682, 866, 889, 1276
GsaI CCCAGC 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 1122
HaeIII GGCC 3 cut(s) 229, 789, 1148
HapII CCGG 7 cut(s) 186, 353, 816, 982, 1116, 1122, 1230
HgaI GACGC 3 cut(s) 722, 894, 1101
HhaI GCGC 4 cut(s) 260, 870, 1029, 1121
Hin1I GRCGYC 3 cut(s) 714, 1112, 1119
Hin1II CATG 8 cut(s) 180, 418, 554, 823, 874, 927, 1198, 1202
Hin6I GCGC 4 cut(s) 258, 868, 1027, 1119
HinP1I GCGC 4 cut(s) 258, 868, 1027, 1119
HincII GTYRAC 2 cut(s) 242, 673
HindII GTYRAC 2 cut(s) 242, 673
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 4 cut(s) 320, 343, 376, 856
HpaII CCGG 7 cut(s) 186, 353, 816, 982, 1116, 1122, 1230
HphI GGTGA 7 cut(s) 28, 52, 307, 838, 916, 1030, 1102
Hpy166II GTNNAC 7 cut(s) 242, 487, 556, 673, 907, 987, 1033
Hpy188I TCNGA 7 cut(s) 81, 328, 363, 628, 708, 1075, 1214
Hpy188III TCNNGA 3 cut(s) 13, 406, 434
Hpy8I GTNNAC 7 cut(s) 242, 487, 556, 673, 907, 987, 1033
Hpy99I CGWCG 4 cut(s) 203, 638, 716, 800
HpyAV CCTTC 5 cut(s) 847, 1006, 1048, 1111, 1138
HpyCH4III ACNGT 2 cut(s) 239, 704
HpyCH4IV ACGT 3 cut(s) 244, 804, 900
HpyF10VI GCNNNNNNNGC 7 cut(s) 213, 257, 388, 424, 871, 888, 1118
HpyF3I CTNAG 4 cut(s) 80, 362, 432, 571
HpySE526I ACGT 3 cut(s) 244, 804, 900
Hsp92I GRCGYC 3 cut(s) 714, 1112, 1119
Hsp92II CATG 8 cut(s) 180, 418, 554, 823, 874, 927, 1198, 1202
HspAI GCGC 4 cut(s) 258, 868, 1027, 1119
KasI GGCGCC 1 cut(s) 1118
Kzo9I GATC 4 cut(s) 76, 708, 939, 1131
LmnI GCTCC 5 cut(s) 436, 476, 948, 1097, 1159
Lsp1109I GCAGC 4 cut(s) 132, 693, 877, 1262
LweI GCATC 4 cut(s) 325, 648, 744, 768
MaeII ACGT 3 cut(s) 244, 804, 900
MaeIII GTNAC 4 cut(s) 151, 313, 826, 1018
MalI GATC 4 cut(s) 78, 710, 941, 1133
MbiI CCGCTC 1 cut(s) 951
MboI GATC 4 cut(s) 76, 708, 939, 1131
MboII GAAGA 5 cut(s) 165, 315, 640, 1096, 1244
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 4 cut(s) 441, 1164, 1179, 1210
MluCI AATT 1 cut(s) 1063
Mly113I GGCGCC 1 cut(s) 1119
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 3 cut(s) 606, 1031, 1053
Mph1103I ATGCAT 1 cut(s) 1200
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 447, 1321
MslI CAYNNNNRTG 7 cut(s) 561, 584, 818, 824, 899, 1088, 1203
MspA1I CMGCKG 2 cut(s) 391, 1172
MspI CCGG 7 cut(s) 186, 353, 816, 982, 1116, 1122, 1230
MspR9I CCNGG 3 cut(s) 354, 877, 974
MvaI CCWGG 2 cut(s) 877, 974
MvnI CGCG 4 cut(s) 888, 938, 1010, 1029
MwoI GCNNNNNNNGC 7 cut(s) 213, 257, 388, 424, 871, 888, 1118
NarI GGCGCC 1 cut(s) 1119
NciI CCSGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 665
NdeII GATC 4 cut(s) 76, 708, 939, 1131
NlaIII CATG 8 cut(s) 180, 418, 554, 823, 874, 927, 1198, 1202
NlaIV GGNNCC 7 cut(s) 655, 727, 956, 993, 1099, 1120, 1161
NmuCI GTSAC 3 cut(s) 313, 826, 1018
NsbI TGCGCA 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 1200
NspI RCATGY 5 cut(s) 418, 554, 823, 874, 1202
OliI CACNNNNGTG 2 cut(s) 561, 584
PaeI GCATGC 3 cut(s) 823, 874, 1202
PaqCI CACCTGC 1 cut(s) 814
PceI AGGCCT 1 cut(s) 1148
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 708
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 343, 376, 856
PinAI ACCGGT 1 cut(s) 981
PkrI GCNGC 5 cut(s) 147, 683, 867, 890, 1277
Ple19I CGATCG 1 cut(s) 942
PleI GAGTC 1 cut(s) 314
PluTI GGCGCC 1 cut(s) 1122
PmaCI CACGTG 1 cut(s) 901
PmlI CACGTG 1 cut(s) 901
PpsI GAGTC 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 901
Psp124BI GAGCTC 1 cut(s) 441
Psp1406I AACGTT 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 875, 972
PspCI CACGTG 1 cut(s) 901
PspEI GGTNACC 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 2 cut(s) 875, 972
PspN4I GGNNCC 7 cut(s) 655, 727, 956, 993, 1099, 1120, 1161
PspPI GGNCC 4 cut(s) 228, 654, 787, 1227
PstI CTGCAG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 76
PvuI CGATCG 1 cut(s) 942
RsaI GTAC 6 cut(s) 28, 85, 305, 935, 986, 1139
RsaNI GTAC 6 cut(s) 27, 84, 304, 934, 985, 1138
RseI CAYNNNNRTG 7 cut(s) 561, 584, 818, 824, 899, 1088, 1203
SacI GAGCTC 1 cut(s) 441
SalI GTCGAC 1 cut(s) 671
SaqAI TTAA 2 cut(s) 447, 1321
SatI GCNGC 5 cut(s) 146, 682, 866, 889, 1276
Sau3AI GATC 4 cut(s) 76, 708, 939, 1131
Sau96I GGNCC 4 cut(s) 228, 654, 787, 1227
ScaI AGTACT 2 cut(s) 85, 305
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 354, 877, 974
SduI GDGCHC 4 cut(s) 441, 1164, 1179, 1210
SfaNI GCATC 4 cut(s) 325, 648, 744, 768
SfcI CTRYAG 3 cut(s) 126, 679, 1150
SfoI GGCGCC 1 cut(s) 1120
SinI GGWCC 2 cut(s) 654, 1227
SmiMI CAYNNNNRTG 7 cut(s) 561, 584, 818, 824, 899, 1088, 1203
SphI GCATGC 3 cut(s) 823, 874, 1202
Sse9I AATT 1 cut(s) 1063
SseBI AGGCCT 1 cut(s) 1148
SsiI CCGC 7 cut(s) 389, 589, 616, 888, 945, 951, 1172
SspDI GGCGCC 1 cut(s) 1118
SstI GAGCTC 1 cut(s) 441
StuI AGGCCT 1 cut(s) 1148
StyD4I CCNGG 3 cut(s) 352, 875, 972
TaaI ACNGT 2 cut(s) 239, 704
TaiI ACGT 3 cut(s) 247, 807, 903
TaqI TCGA 3 cut(s) 198, 672, 711
TasI AATT 1 cut(s) 1063
TatI WGTACW 2 cut(s) 83, 303
TauI GCSGC 1 cut(s) 891
TfiI GAWTC 3 cut(s) 343, 376, 856
Tru1I TTAA 2 cut(s) 447, 1321
Tru9I TTAA 2 cut(s) 447, 1321
TscAI CASTG 4 cut(s) 683, 698, 925, 1274
TseFI GTSAC 3 cut(s) 313, 826, 1018
TseI GCWGC 4 cut(s) 145, 681, 865, 1275
Tsp45I GTSAC 3 cut(s) 313, 826, 1018
TspDTI ATGAA 4 cut(s) 10, 17, 85, 165
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 4 cut(s) 683, 698, 925, 1274
VpaK11BI GGWCC 2 cut(s) 654, 1227
XceI RCATGY 5 cut(s) 418, 554, 823, 874, 1202
XcmI CCANNNNNNNNNTGG 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 8
ZrmI AGTACT 2 cut(s) 85, 305
Zsp2I ATGCAT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.