FvH4_6g20260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013698
Physical Location & Seq
Reverse (-)
13857852 .. 13859135
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20260.t1

Sequence Viewer

Length: 726 bp
ATGATCGTGCCAGAGATGATGGAGGCCATCGGGGCCATCTCTTTCCCGGTGGTGTTCTGCGACGGCGAGAGCGAGTCGGAAATCGGAAACGTCGTGGTTTACCCGGCGATGGAGTTCAAGCGGTTCCAGGCGGTTCTGAGCCAGAAGATCGGAATCTCGCCGAACCAGTTTTCGGTGTTCATATCCTCGCCGGAGTCCCGCCGGCGAATCCCCGTCACGAGCAAGATCAACTTCAGCGTCATCTCTCGCGAGACCAACTGCTTCTTCATCGTCGTCCTGAAGCGGACCAAGCGCGAGCGTCGCCGGCAAAAAAACAGTCAGCACCACCTCGACTACAAAACGGCGTCGTATTACTCCCCCGGTCCAGTCATGGAGAACCCTCTTGAAAATGCCGTGTTCCTGAGGCGCGGCATGGAGATCAACTACGTCCGGTCGGGCATGCCTTTCGTGGGTCGGGTCGGGTGGGAGAAGCGGGTCAGGGAGTTGCAGATGGAAAAGGAGAGGTATTTGATGAGCATGGGCCTGGTAAGGGCTTCCGGGTTTGGGTTTGGCCAGAGCGGCGGCGGTAACGGTGGAGGAGGAGGTGGCGTGGTGGTGTGTGAGGAATGTTCCAGAGCTGAGGAGATAGGGACGGAGGTTGGGTTCCATTGGTGCGCTTACGATCCTGTTGTCACTATCCAGTTCAGGTCGCCCGCGGGTCCAATTTCCCGACCCACCACCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.98

Weight (kDa)

9.3

Isoelectric Point (pI)

69.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7138 PF23596 13 - 91 6.9e-38 Domain of unknown function (DUF7138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20260
malus_domestica MD12G1039700.v1.1 MD14G1038300.v1.1
prunus_persica Prupe.7G095200_v2.0.a1
pyrus_communis pycom03g00540 pycom12g03370 pycom14g03320
rosa_chinensis RchiOBHm_Chr3g0475851
rosa_laevigata RLG00000023807
rosa_multiflora Rmu_co8277165.1_g000001
rosa_roxburghii Rroxscaffold_6G00405790
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3BG232000 Rh3CG226600 Rh3DG227200 Rh4CG218200 Rh7BG133300
rosa_wichuraiana Rw3G018360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 558
AccII CGCG 4 cut(s) 249, 294, 408, 695
AclWI GGATC 1 cut(s) 656
AcoI YGGCCR 1 cut(s) 550
AcuI CTGAAG 2 cut(s) 217, 299
AcyI GRCGYC 1 cut(s) 344
AfiI CCNNNNNNNGG 5 cut(s) 109, 172, 449, 529, 543
AgsI TTSAA 2 cut(s) 118, 386
AjnI CCWGG 2 cut(s) 126, 522
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AloI GAACNNNNNNTCC 2 cut(s) 626, 658
AluBI AGCT 1 cut(s) 617
AluI AGCT 1 cut(s) 617
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 656
AoxI GGCC 4 cut(s) 24, 33, 520, 550
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AspLEI GCGC 3 cut(s) 294, 408, 656
AspS9I GGNCC 5 cut(s) 33, 285, 362, 520, 698
AsuC2I CCSGG 4 cut(s) 47, 104, 360, 538
AvaII GGWCC 3 cut(s) 285, 362, 698
AxyI CCTNAGG 1 cut(s) 401
BalI TGGCCA 1 cut(s) 552
BauI CACGAG 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 618
BccI CCATC 5 cut(s) 13, 35, 44, 103, 484
BceAI ACGGC 3 cut(s) 79, 357, 377
BcgI CGANNNNNNTGC 2 cut(s) 427, 461
BciT130I CCWGG 2 cut(s) 128, 524
BcnI CCSGG 4 cut(s) 47, 104, 360, 538
BcoDI GTCTC 1 cut(s) 245
BglI GCCNNNNNGGC 2 cut(s) 32, 558
BisI GCNGC 3 cut(s) 409, 559, 562
BlsI GCNGC 3 cut(s) 410, 560, 563
Bme1390I CCNGG 6 cut(s) 47, 104, 128, 360, 524, 538
Bme18I GGWCC 3 cut(s) 285, 362, 698
BmgT120I GGNCC 5 cut(s) 33, 285, 362, 520, 698
BmiI GGNNCC 4 cut(s) 34, 125, 644, 699
BmrFI CCNGG 6 cut(s) 47, 104, 128, 360, 524, 538
Bpu10I CCTNAGC 1 cut(s) 618
BpuMI CCSGG 4 cut(s) 47, 104, 360, 538
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 344
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 358, 693
BsaWI WCCGGW 1 cut(s) 429
BsaXI ACNNNNNCTCC 4 cut(s) 573, 603, 626, 656
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 172, 449, 529, 543
Bse118I RCCGGY 2 cut(s) 201, 303
Bse1I ACTGG 3 cut(s) 166, 365, 679
Bse21I CCTNAGG 1 cut(s) 401
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 2 cut(s) 128, 524
BseDI CCNNGG 2 cut(s) 358, 693
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 5 cut(s) 109, 172, 449, 529, 543
BseMII CTCAG 3 cut(s) 128, 392, 609
BseNI ACTGG 3 cut(s) 166, 365, 679
BseRI GAGGAG 3 cut(s) 591, 594, 635
Bsh1236I CGCG 4 cut(s) 249, 294, 408, 695
Bsh1285I CGRYCG 1 cut(s) 434
BshFI GGCC 4 cut(s) 26, 35, 522, 552
BsiEI CGRYCG 1 cut(s) 434
BsiSI CCGG 8 cut(s) 47, 104, 191, 202, 304, 360, 430, 537
BslFI GGGAC 2 cut(s) 181, 643
BslI CCNNNNNNNGG 5 cut(s) 109, 172, 449, 529, 543
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 2 cut(s) 181, 643
BsnI GGCC 4 cut(s) 26, 35, 522, 552
Bso31I GGTCTC 1 cut(s) 245
Bsp143I GATC 5 cut(s) 3, 147, 225, 417, 661
Bsp68I TCGCGA 1 cut(s) 249
BspANI GGCC 4 cut(s) 26, 35, 522, 552
BspCNI CTCAG 3 cut(s) 129, 393, 610
BspFNI CGCG 4 cut(s) 249, 294, 408, 695
BspLI GGNNCC 4 cut(s) 34, 125, 644, 699
BspPI GGATC 1 cut(s) 656
BspTNI GGTCTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 558
BsrFI RCCGGY 2 cut(s) 201, 303
BsrI ACTGG 3 cut(s) 166, 365, 679
BssAI RCCGGY 2 cut(s) 201, 303
BssECI CCNNGG 2 cut(s) 358, 693
BssMI GATC 5 cut(s) 3, 147, 225, 417, 661
BssNI GRCGYC 1 cut(s) 344
BssSI CACGAG 1 cut(s) 217
Bst2BI CACGAG 1 cut(s) 217
Bst2UI CCWGG 2 cut(s) 128, 524
Bst4CI ACNGT 2 cut(s) 317, 572
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 5 cut(s) 203, 296, 305, 440, 693
BstDEI CTNAG 3 cut(s) 137, 401, 618
BstDSI CCRYGG 1 cut(s) 693
BstFNI CGCG 4 cut(s) 249, 294, 408, 695
BstHHI GCGC 3 cut(s) 294, 408, 656
BstKTI GATC 5 cut(s) 6, 150, 228, 420, 664
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 5 cut(s) 3, 147, 225, 417, 661
BstMCI CGRYCG 1 cut(s) 434
BstMWI GCNNNNNNNGC 5 cut(s) 32, 289, 300, 304, 558
BstNI CCWGG 2 cut(s) 128, 524
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 6 cut(s) 45, 102, 126, 358, 522, 536
BstUI CGCG 4 cut(s) 249, 294, 408, 695
Bsu36I CCTNAGG 1 cut(s) 401
BsuRI GGCC 4 cut(s) 26, 35, 522, 552
BtgI CCRYGG 1 cut(s) 693
BtgZI GCGATG 1 cut(s) 122
BtuMI TCGCGA 1 cut(s) 249
Cac8I GCNNGC 5 cut(s) 203, 296, 305, 440, 693
CfoI GCGC 3 cut(s) 294, 408, 656
Cfr10I RCCGGY 2 cut(s) 201, 303
Cfr13I GGNCC 5 cut(s) 33, 285, 362, 520, 698
Cfr42I CCGCGG 1 cut(s) 696
CseI GACGC 3 cut(s) 226, 287, 333
CviAII CATG 4 cut(s) 370, 412, 439, 517
CviJI RGCY 7 cut(s) 26, 35, 141, 522, 533, 552, 617
CviKI_1 RGCY 7 cut(s) 26, 35, 141, 522, 533, 552, 617
DdeI CTNAG 3 cut(s) 137, 401, 618
DpnI GATC 5 cut(s) 5, 149, 227, 419, 663
DpnII GATC 5 cut(s) 3, 147, 225, 417, 661
EaeI YGGCCR 1 cut(s) 550
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 3 cut(s) 285, 362, 698
Eco57I CTGAAG 2 cut(s) 217, 299
Eco81I CCTNAGG 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 126, 522
FaeI CATG 4 cut(s) 373, 415, 442, 520
FaiI YATR 5 cut(s) 182, 371, 413, 440, 518
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 2 cut(s) 181, 643
FatI CATG 4 cut(s) 369, 411, 438, 516
FauI CCCGC 4 cut(s) 206, 465, 688, 700
Fnu4HI GCNGC 3 cut(s) 409, 559, 562
Fsp4HI GCNGC 3 cut(s) 409, 559, 562
GlaI GCGC 3 cut(s) 293, 407, 655
GluI GCNGC 3 cut(s) 409, 559, 562
HaeIII GGCC 4 cut(s) 26, 35, 522, 552
HapII CCGG 8 cut(s) 47, 104, 191, 202, 304, 360, 430, 537
HgaI GACGC 3 cut(s) 226, 287, 333
HhaI GCGC 3 cut(s) 294, 408, 656
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 4 cut(s) 373, 415, 442, 520
Hin6I GCGC 3 cut(s) 292, 406, 654
HinP1I GCGC 3 cut(s) 292, 406, 654
HinfI GANTC 4 cut(s) 74, 153, 194, 207
HpaII CCGG 8 cut(s) 47, 104, 191, 202, 304, 360, 430, 537
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 4 cut(s) 79, 86, 138, 152
Hpy188III TCNNGA 7 cut(s) 217, 248, 277, 383, 400, 612, 708
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 5 cut(s) 65, 95, 275, 303, 349
HpyCH4III ACNGT 2 cut(s) 317, 572
HpyCH4IV ACGT 2 cut(s) 90, 426
HpyCH4V TGCA 1 cut(s) 487
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 289, 300, 304, 558
HpyF3I CTNAG 3 cut(s) 137, 401, 618
HpySE526I ACGT 2 cut(s) 90, 426
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 4 cut(s) 373, 415, 442, 520
HspAI GCGC 3 cut(s) 292, 406, 654
KroI GCCGGC 2 cut(s) 201, 303
KroNI GCCGGC 2 cut(s) 203, 305
KspI CCGCGG 1 cut(s) 696
Kzo9I GATC 5 cut(s) 3, 147, 225, 417, 661
MaeII ACGT 2 cut(s) 90, 426
MaeIII GTNAC 3 cut(s) 214, 566, 670
MalI GATC 5 cut(s) 5, 149, 227, 419, 663
MbiI CCGCTC 1 cut(s) 558
MboI GATC 5 cut(s) 3, 147, 225, 417, 661
MboII GAAGA 2 cut(s) 157, 256
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 1 cut(s) 702
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 2 cut(s) 83, 203
MmeI TCCRAC 1 cut(s) 57
Mox20I TGGCCA 1 cut(s) 552
MreI CGCCGGCG 1 cut(s) 201
MroNI GCCGGC 2 cut(s) 201, 303
MscI TGGCCA 1 cut(s) 552
MseI TTAA 1 cut(s) 724
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 8 cut(s) 47, 104, 191, 202, 304, 360, 430, 537
MspR9I CCNGG 6 cut(s) 47, 104, 128, 360, 524, 538
MvaI CCWGG 2 cut(s) 128, 524
MvnI CGCG 4 cut(s) 249, 294, 408, 695
MwoI GCNNNNNNNGC 5 cut(s) 32, 289, 300, 304, 558
NaeI GCCGGC 2 cut(s) 203, 305
NciI CCSGG 4 cut(s) 47, 104, 360, 538
NdeII GATC 5 cut(s) 3, 147, 225, 417, 661
NgoMIV GCCGGC 2 cut(s) 201, 303
NlaIII CATG 4 cut(s) 373, 415, 442, 520
NlaIV GGNNCC 4 cut(s) 34, 125, 644, 699
NmuCI GTSAC 2 cut(s) 214, 670
NruI TCGCGA 1 cut(s) 249
NspI RCATGY 1 cut(s) 442
PaeI GCATGC 1 cut(s) 442
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 90
PdiI GCCGGC 2 cut(s) 203, 305
PfeI GAWTC 2 cut(s) 153, 207
PkrI GCNGC 3 cut(s) 410, 560, 563
PleI GAGTC 2 cut(s) 82, 202
PpsI GAGTC 2 cut(s) 82, 202
Psp6I CCWGG 2 cut(s) 126, 522
PspGI CCWGG 2 cut(s) 126, 522
PspN4I GGNNCC 4 cut(s) 34, 125, 644, 699
PspPI GGNCC 5 cut(s) 33, 285, 362, 520, 698
RruI TCGCGA 1 cut(s) 249
SacII CCGCGG 1 cut(s) 696
SaqAI TTAA 1 cut(s) 724
SatI GCNGC 3 cut(s) 409, 559, 562
Sau3AI GATC 5 cut(s) 3, 147, 225, 417, 661
Sau96I GGNCC 5 cut(s) 33, 285, 362, 520, 698
SchI GAGTC 2 cut(s) 83, 203
ScrFI CCNGG 6 cut(s) 47, 104, 128, 360, 524, 538
SetI ASST 8 cut(s) 93, 330, 429, 506, 586, 619, 639, 689
SfiI GGCCNNNNNGGCC 1 cut(s) 32
Sfr303I CCGCGG 1 cut(s) 696
SgrAI CRCCGGYG 1 cut(s) 201
SgrBI CCGCGG 1 cut(s) 696
SinI GGWCC 3 cut(s) 285, 362, 698
SphI GCATGC 1 cut(s) 442
Sse9I AATT 1 cut(s) 702
StyD4I CCNGG 6 cut(s) 45, 102, 126, 358, 522, 536
TaaI ACNGT 2 cut(s) 317, 572
TaiI ACGT 2 cut(s) 93, 429
TaqI TCGA 1 cut(s) 330
TasI AATT 1 cut(s) 702
TauI GCSGC 3 cut(s) 411, 561, 564
TfiI GAWTC 2 cut(s) 153, 207
Tru1I TTAA 1 cut(s) 724
Tru9I TTAA 1 cut(s) 724
TseFI GTSAC 2 cut(s) 214, 670
Tsp45I GTSAC 2 cut(s) 214, 670
TspDTI ATGAA 2 cut(s) 169, 256
TspGWI ACGGA 1 cut(s) 647
VpaK11BI GGWCC 3 cut(s) 285, 362, 698
XceI RCATGY 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.