Rh3DG227200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0013698
Physical Location & Seq
Reverse (-)
21454645 .. 21456442
1798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG227200.1

Sequence Viewer

Length: 747 bp
ATGATGGAGGCCATCGGAGGCATCTCTTTTCCGGTCGTGTTCTGCGACGGCGAGAGCGAGTCGGAAATCGGAGACGTCGTGATTTACCCGGCGATGGAGTTCAAGCGGTTCCAGGCGGTTCTGAGCCAGAAGATCGGCATCTCACCGAACCAGTTCTCGGTGTTCATCTCCTCGCCGGAATCCGGCCGGCGAATCCCCGTCACGAGCAAGATCAACTTCAGCGTGATCTCCCGCGAGACCAACTGCTTCTTTCTCGTCGTCTTGAAGCGGACCAAGCGCGAGCGTCGCCGGAGGAATAATCAGCACCACCACCTCGACGAGTCCCAGTACTACAATACGCCGTCGTCGTATTACTCCCCCAATAAAATCAAGGAGAACCCTCTGGAGAATGCTGTGTTTCTGAGGCGCGGCGCGGGGATCAACCACCACGGCGGGGAAGCAGCAGCGCTTTCCGGGCTGGGCATGCCGTTCGCGGGTCGGGTCGAGTGGGAGAACCGGGTCAGGGAGTTGCAGATTGAAAAGGAGAGATATTTGATGAACATGGGCCTTGCGAGCGCTTCTGGGTTTGGGCTGGGCCAGGGCGGCGGCGGTGACGGTGGAGGCGGCGGCGTGGTGGTGTGCGAGGAATGTTCCAGAGCTGATGAGATGGGGACGGAGGTTGGGTTCCATTGGTGCGCTTACGATCCTGTTGTGACTTTCCGGTTCAGGTCTCCCGCGGGTCCAATCTCCCGACCCACCACCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

248

Amino Acids

27.43

Weight (kDa)

8.28

Isoelectric Point (pI)

60.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7138 PF23596 8 - 86 1.5e-37 Domain of unknown function (DUF7138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20260
malus_domestica MD12G1039700.v1.1 MD14G1038300.v1.1
prunus_persica Prupe.7G095200_v2.0.a1
pyrus_communis pycom03g00540 pycom12g03370 pycom14g03320
rosa_chinensis RchiOBHm_Chr3g0475851
rosa_laevigata RLG00000023807
rosa_multiflora Rmu_co8277165.1_g000001
rosa_roxburghii Rroxscaffold_6G00405790
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3BG232000 Rh3CG226600 Rh3DG227200 Rh4CG218200 Rh7BG133300
rosa_wichuraiana Rw3G018360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 78
AccII CGCG 6 cut(s) 234, 279, 408, 413, 473, 716
AclWI GGATC 2 cut(s) 425, 677
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 1 cut(s) 329
AfeI AGCGCT 2 cut(s) 447, 556
AfiI CCNNNNNNNGG 6 cut(s) 94, 157, 182, 433, 473, 502
AgsI TTSAA 3 cut(s) 103, 265, 518
AjnI CCWGG 2 cut(s) 111, 576
AloI GAACNNNNNNTCC 2 cut(s) 647, 679
AluBI AGCT 1 cut(s) 638
AluI AGCT 1 cut(s) 638
Alw26I GTCTC 3 cut(s) 66, 230, 714
AlwI GGATC 2 cut(s) 425, 677
Aor51HI AGCGCT 2 cut(s) 447, 556
AoxI GGCC 4 cut(s) 9, 184, 544, 574
ApeKI GCWGC 2 cut(s) 440, 443
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 6 cut(s) 279, 408, 413, 448, 557, 677
AspS9I GGNCC 4 cut(s) 270, 544, 574, 719
AsuC2I CCSGG 3 cut(s) 89, 454, 497
AsuHPI GGTGA 2 cut(s) 135, 602
AvaII GGWCC 2 cut(s) 270, 719
BauI CACGAG 1 cut(s) 202
BbvI GCAGC 2 cut(s) 452, 455
BccI CCATC 3 cut(s) 20, 88, 640
BceAI ACGGC 4 cut(s) 64, 325, 445, 451
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BciT130I CCWGG 2 cut(s) 113, 578
BcnI CCSGG 3 cut(s) 89, 454, 497
BcoDI GTCTC 3 cut(s) 66, 230, 714
BfoI RGCGCY 2 cut(s) 449, 558
BglI GCCNNNNNGGC 1 cut(s) 582
BisI GCNGC 7 cut(s) 409, 441, 444, 583, 586, 604, 607
BlsI GCNGC 7 cut(s) 410, 442, 445, 584, 587, 605, 608
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 5 cut(s) 89, 113, 454, 497, 578
Bme18I GGWCC 2 cut(s) 270, 719
BmgT120I GGNCC 4 cut(s) 270, 544, 574, 719
BmiI GGNNCC 3 cut(s) 110, 665, 720
BmrFI CCNGG 5 cut(s) 89, 113, 454, 497, 578
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 2 cut(s) 30, 147
BmuI ACTGGG 1 cut(s) 319
BpmI CTGGAG 1 cut(s) 404
BpuMI CCSGG 3 cut(s) 89, 454, 497
BsaBI GATNNNNATC 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 2 cut(s) 230, 714
BsaJI CCNNGG 3 cut(s) 427, 577, 714
BsaWI WCCGGW 2 cut(s) 31, 699
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 647, 677
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 157, 182, 433, 473, 502
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 151, 325
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 113, 578
BseDI CCNNGG 3 cut(s) 427, 577, 714
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 6 cut(s) 94, 157, 182, 433, 473, 502
BseMII CTCAG 2 cut(s) 113, 392
BseNI ACTGG 2 cut(s) 151, 325
BseRI GAGGAG 1 cut(s) 160
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 2 cut(s) 452, 455
BseYI CCCAGC 2 cut(s) 457, 571
Bsh1236I CGCG 6 cut(s) 234, 279, 408, 413, 473, 716
Bsh1285I CGRYCG 2 cut(s) 36, 187
BshFI GGCC 4 cut(s) 11, 186, 546, 576
BsiEI CGRYCG 2 cut(s) 36, 187
BsiSI CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 453, 496, 700
BslFI GGGAC 2 cut(s) 307, 664
BslI CCNNNNNNNGG 6 cut(s) 94, 157, 182, 433, 473, 502
BsmAI GTCTC 3 cut(s) 66, 230, 714
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 2 cut(s) 307, 664
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 4 cut(s) 11, 186, 546, 576
Bso31I GGTCTC 2 cut(s) 230, 714
Bsp143I GATC 5 cut(s) 132, 210, 225, 417, 682
BspANI GGCC 4 cut(s) 11, 186, 546, 576
BspCNI CTCAG 2 cut(s) 114, 393
BspFNI CGCG 6 cut(s) 234, 279, 408, 413, 473, 716
BspLI GGNNCC 3 cut(s) 110, 665, 720
BspPI GGATC 2 cut(s) 425, 677
BspTNI GGTCTC 2 cut(s) 230, 714
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 2 cut(s) 151, 325
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 427, 577, 714
BssMI GATC 5 cut(s) 132, 210, 225, 417, 682
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 202
Bst2BI CACGAG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 113, 578
Bst4CI ACNGT 1 cut(s) 596
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 4 cut(s) 188, 281, 464, 553
BstDEI CTNAG 2 cut(s) 122, 401
BstDSI CCRYGG 2 cut(s) 427, 714
BstFNI CGCG 6 cut(s) 234, 279, 408, 413, 473, 716
BstH2I RGCGCY 2 cut(s) 449, 558
BstHHI GCGC 6 cut(s) 279, 408, 413, 448, 557, 677
BstKTI GATC 5 cut(s) 135, 213, 228, 420, 685
BstMAI GTCTC 3 cut(s) 66, 230, 714
BstMBI GATC 5 cut(s) 132, 210, 225, 417, 682
BstMCI CGRYCG 2 cut(s) 36, 187
BstMWI GCNNNNNNNGC 6 cut(s) 274, 285, 454, 463, 552, 582
BstNI CCWGG 2 cut(s) 113, 578
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 5 cut(s) 87, 111, 452, 495, 576
BstUI CGCG 6 cut(s) 234, 279, 408, 413, 473, 716
BstV1I GCAGC 2 cut(s) 452, 455
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 4 cut(s) 11, 186, 546, 576
BtgI CCRYGG 2 cut(s) 427, 714
BtgZI GCGATG 1 cut(s) 107
Cac8I GCNNGC 4 cut(s) 188, 281, 464, 553
CfoI GCGC 6 cut(s) 279, 408, 413, 448, 557, 677
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 4 cut(s) 270, 544, 574, 719
Cfr42I CCGCGG 1 cut(s) 717
CseI GACGC 1 cut(s) 272
Csp6I GTAC 1 cut(s) 328
CviAII CATG 2 cut(s) 463, 541
CviJI RGCY 8 cut(s) 11, 126, 186, 457, 546, 571, 576, 638
CviKI_1 RGCY 8 cut(s) 11, 126, 186, 457, 546, 571, 576, 638
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 2 cut(s) 122, 401
DpnI GATC 5 cut(s) 134, 212, 227, 419, 684
DpnII GATC 5 cut(s) 132, 210, 225, 417, 682
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
EclXI CGGCCG 1 cut(s) 184
Eco31I GGTCTC 2 cut(s) 230, 714
Eco47I GGWCC 2 cut(s) 270, 719
Eco47III AGCGCT 2 cut(s) 447, 556
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 111, 576
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 2 cut(s) 466, 544
FaiI YATR 2 cut(s) 464, 542
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 2 cut(s) 307, 664
FatI CATG 2 cut(s) 462, 540
FauI CCCGC 6 cut(s) 239, 406, 425, 466, 709, 721
Fnu4HI GCNGC 7 cut(s) 409, 441, 444, 583, 586, 604, 607
Fsp4HI GCNGC 7 cut(s) 409, 441, 444, 583, 586, 604, 607
GlaI GCGC 6 cut(s) 278, 407, 412, 447, 556, 676
GluI GCNGC 7 cut(s) 409, 441, 444, 583, 586, 604, 607
GsaI CCCAGC 2 cut(s) 461, 575
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 2 cut(s) 449, 558
HaeIII GGCC 4 cut(s) 11, 186, 546, 576
HapII CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 453, 496, 700
HgaI GACGC 1 cut(s) 272
HhaI GCGC 6 cut(s) 279, 408, 413, 448, 557, 677
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 466, 544
Hin6I GCGC 6 cut(s) 277, 406, 411, 446, 555, 675
HinP1I GCGC 6 cut(s) 277, 406, 411, 446, 555, 675
HinfI GANTC 4 cut(s) 59, 179, 192, 320
HpaII CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 453, 496, 700
HphI GGTGA 2 cut(s) 135, 602
Hpy188I TCNGA 5 cut(s) 17, 64, 71, 123, 402
Hpy188III TCNNGA 6 cut(s) 79, 202, 262, 383, 633, 729
Hpy99I CGWCG 7 cut(s) 50, 80, 260, 288, 320, 346, 349
HpyCH4III ACNGT 1 cut(s) 596
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 1 cut(s) 511
HpyF10VI GCNNNNNNNGC 6 cut(s) 274, 285, 454, 463, 552, 582
HpyF3I CTNAG 2 cut(s) 122, 401
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 466, 544
HspAI GCGC 6 cut(s) 277, 406, 411, 446, 555, 675
KroI GCCGGC 1 cut(s) 186
KroNI GCCGGC 1 cut(s) 188
KspI CCGCGG 1 cut(s) 717
Kzo9I GATC 5 cut(s) 132, 210, 225, 417, 682
Lsp1109I GCAGC 2 cut(s) 452, 455
LweI GCATC 2 cut(s) 30, 147
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 199, 590, 691
MalI GATC 5 cut(s) 134, 212, 227, 419, 684
MboI GATC 5 cut(s) 132, 210, 225, 417, 682
MboII GAAGA 1 cut(s) 142
MlyI GAGTC 2 cut(s) 68, 329
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 9 cut(s) 11, 181, 285, 323, 390, 396, 593, 616, 649
MroNI GCCGGC 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 745
MspA1I CMGCKG 1 cut(s) 716
MspI CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 453, 496, 700
MspR9I CCNGG 5 cut(s) 89, 113, 454, 497, 578
MteI GCGCNGCGC 1 cut(s) 409
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 2 cut(s) 113, 578
MvnI CGCG 6 cut(s) 234, 279, 408, 413, 473, 716
MwoI GCNNNNNNNGC 6 cut(s) 274, 285, 454, 463, 552, 582
NaeI GCCGGC 1 cut(s) 188
NciI CCSGG 3 cut(s) 89, 454, 497
NdeII GATC 5 cut(s) 132, 210, 225, 417, 682
NgoMIV GCCGGC 1 cut(s) 186
NlaIII CATG 2 cut(s) 466, 544
NlaIV GGNNCC 3 cut(s) 110, 665, 720
NmuCI GTSAC 3 cut(s) 199, 590, 691
NspI RCATGY 1 cut(s) 466
PaeI GCATGC 1 cut(s) 466
PcsI WCGNNNNNNNCGW 4 cut(s) 42, 54, 75, 344
PctI GAATGC 1 cut(s) 394
PdiI GCCGGC 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 179, 192
PkrI GCNGC 7 cut(s) 410, 442, 445, 584, 587, 605, 608
PleI GAGTC 2 cut(s) 67, 328
PpsI GAGTC 2 cut(s) 67, 328
Psp6I CCWGG 2 cut(s) 111, 576
PspFI CCCAGC 2 cut(s) 457, 571
PspGI CCWGG 2 cut(s) 111, 576
PspN4I GGNNCC 3 cut(s) 110, 665, 720
PspPI GGNCC 4 cut(s) 270, 544, 574, 719
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SacII CCGCGG 1 cut(s) 717
SaqAI TTAA 1 cut(s) 745
SatI GCNGC 7 cut(s) 409, 441, 444, 583, 586, 604, 607
Sau3AI GATC 5 cut(s) 132, 210, 225, 417, 682
Sau96I GGNCC 4 cut(s) 270, 544, 574, 719
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 2 cut(s) 68, 329
ScrFI CCNGG 5 cut(s) 89, 113, 454, 497, 578
SetI ASST 5 cut(s) 78, 315, 640, 660, 710
SfaNI GCATC 2 cut(s) 30, 147
Sfr303I CCGCGG 1 cut(s) 717
SgrBI CCGCGG 1 cut(s) 717
SinI GGWCC 2 cut(s) 270, 719
SphI GCATGC 1 cut(s) 466
StyD4I CCNGG 5 cut(s) 87, 111, 452, 495, 576
TaaI ACNGT 1 cut(s) 596
TaiI ACGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 315, 483
TatI WGTACW 1 cut(s) 327
TauI GCSGC 5 cut(s) 411, 585, 588, 606, 609
TfiI GAWTC 2 cut(s) 179, 192
Tru1I TTAA 1 cut(s) 745
Tru9I TTAA 1 cut(s) 745
TseFI GTSAC 3 cut(s) 199, 590, 691
TseI GCWGC 2 cut(s) 440, 443
Tsp45I GTSAC 3 cut(s) 199, 590, 691
TspDTI ATGAA 2 cut(s) 154, 551
TspGWI ACGGA 1 cut(s) 668
VpaK11BI GGWCC 2 cut(s) 270, 719
XceI RCATGY 1 cut(s) 466
XmnI GAANNNNTTC 1 cut(s) 152
ZraI GACGTC 1 cut(s) 76
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.