Rroxscaffold_6G00405790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013698
Physical Location & Seq
Forward (+)
28279793 .. 28280536
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405790.1

Sequence Viewer

Length: 744 bp
ATGATGGATACCATCGGAGGCATCTCTTTCCCGGTCGTGTTCTGCGACGGCGAGAGCGAGTCGGAAATCGGAGACGTTGTGATTTACCCGGCGATGGAGTTCAAGCGGTTCCAGGCGGTTCTGAGCCAGAAGATCGGAATCTCACCGAACCAGTTCTCGGTGTTCATCTCCTCGCCGGAATCCGGCCGGCGAATCCCCGTCACGAGCAAGATCAACTTCAGCGTGATCTCCCGCGAGACCAACTGCTTCTTTCTCGTGGTCTTGAAGCGGACCAAGCGCGAGCGTCGCCGGAGGAATAATCAGCACCACCACCTCGACGAGTCCCAGTACTACAATACGCCGTCGTATTACTCCCCCAATAAAATCAAGGAGAACCCTCTGGAGAATGCTGTGTTTCTGAGGCGCGGCACGGGGATCAACCACCAAGGCGGGGAAGCAGCAGCGTTTTCCGGGATGGGCATGCCGTTCGCGGGTCGGGTCGAGTGGGAGAACCGGGTCAGGGAGTTGCAGATTGAGAAGGAGAGATATTTGATGAACATGGGCCTTGCGAGGGCTTCTGGGTTTGGGCTGGGCCAGGGCGGCGGCGGTGACGGTGGAGGTGGCGGCGTGGTGGTGTGTGAGGAATGTTCCAGAGCTGATGAGATGGGGACGGAGGTTGGGTTCCATTGGTGCGCTTACGATCCTGTTGTGACATTCCGGTTCAGGTCGCCCGCGGGTCCAATCTCCAGACCCACCACCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

27.45

Weight (kDa)

8.21

Isoelectric Point (pI)

59.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7138 PF23596 8 - 86 1.5e-37 Domain of unknown function (DUF7138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20260
malus_domestica MD12G1039700.v1.1 MD14G1038300.v1.1
prunus_persica Prupe.7G095200_v2.0.a1
pyrus_communis pycom03g00540 pycom12g03370 pycom14g03320
rosa_chinensis RchiOBHm_Chr3g0475851
rosa_laevigata RLG00000023807
rosa_multiflora Rmu_co8277165.1_g000001
rosa_roxburghii Rroxscaffold_6G00405790
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3BG232000 Rh3CG226600 Rh3DG227200 Rh4CG218200 Rh7BG133300
rosa_wichuraiana Rw3G018360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 234, 279, 405, 470, 713
AclWI GGATC 2 cut(s) 422, 674
AcoI YGGCCR 1 cut(s) 184
AcuI CTGAAG 1 cut(s) 202
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 7 cut(s) 94, 157, 182, 430, 470, 499, 550
AgsI TTSAA 2 cut(s) 103, 265
AjnI CCWGG 2 cut(s) 111, 573
AloI GAACNNNNNNTCC 2 cut(s) 644, 676
AluBI AGCT 1 cut(s) 635
AluI AGCT 1 cut(s) 635
Alw26I GTCTC 2 cut(s) 66, 230
AlwI GGATC 2 cut(s) 422, 674
AoxI GGCC 3 cut(s) 184, 541, 571
ApeKI GCWGC 2 cut(s) 437, 440
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 3 cut(s) 279, 405, 674
AspS9I GGNCC 4 cut(s) 270, 541, 571, 716
AsuC2I CCSGG 4 cut(s) 32, 89, 451, 494
AsuHPI GGTGA 2 cut(s) 135, 599
AvaII GGWCC 2 cut(s) 270, 716
BauI CACGAG 2 cut(s) 202, 254
BbvI GCAGC 2 cut(s) 449, 452
BccI CCATC 4 cut(s) 20, 88, 448, 637
BceAI ACGGC 3 cut(s) 64, 325, 448
BcgI CGANNNNNNTGC 2 cut(s) 448, 482
BciT130I CCWGG 2 cut(s) 113, 575
BcnI CCSGG 4 cut(s) 32, 89, 451, 494
BcoDI GTCTC 2 cut(s) 66, 230
BglI GCCNNNNNGGC 1 cut(s) 579
BisI GCNGC 6 cut(s) 406, 438, 441, 580, 583, 604
BlsI GCNGC 6 cut(s) 407, 439, 442, 581, 584, 605
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 6 cut(s) 32, 89, 113, 451, 494, 575
Bme18I GGWCC 2 cut(s) 270, 716
BmgT120I GGNCC 4 cut(s) 270, 541, 571, 716
BmiI GGNNCC 3 cut(s) 110, 662, 717
BmrFI CCNGG 6 cut(s) 32, 89, 113, 451, 494, 575
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 1 cut(s) 30
BmuI ACTGGG 1 cut(s) 319
BpmI CTGGAG 2 cut(s) 401, 709
BpuMI CCSGG 4 cut(s) 32, 89, 451, 494
BsaBI GATNNNNATC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 3 cut(s) 424, 574, 711
BsaWI WCCGGW 1 cut(s) 696
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 644, 674
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 157, 182, 430, 470, 499, 550
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 151, 325
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 113, 575
BseDI CCNNGG 3 cut(s) 424, 574, 711
BseGI GGATG 1 cut(s) 459
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 7 cut(s) 94, 157, 182, 430, 470, 499, 550
BseMII CTCAG 2 cut(s) 113, 389
BseNI ACTGG 2 cut(s) 151, 325
BseRI GAGGAG 1 cut(s) 160
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 2 cut(s) 449, 452
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 5 cut(s) 234, 279, 405, 470, 713
Bsh1285I CGRYCG 2 cut(s) 36, 187
BshFI GGCC 3 cut(s) 186, 543, 573
BsiEI CGRYCG 2 cut(s) 36, 187
BsiSI CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 450, 493, 697
BslFI GGGAC 2 cut(s) 307, 661
BslI CCNNNNNNNGG 7 cut(s) 94, 157, 182, 430, 470, 499, 550
BsmAI GTCTC 2 cut(s) 66, 230
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 2 cut(s) 307, 661
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 3 cut(s) 186, 543, 573
Bso31I GGTCTC 1 cut(s) 230
Bsp143I GATC 5 cut(s) 132, 210, 225, 414, 679
BspANI GGCC 3 cut(s) 186, 543, 573
BspCNI CTCAG 2 cut(s) 114, 390
BspFNI CGCG 5 cut(s) 234, 279, 405, 470, 713
BspLI GGNNCC 3 cut(s) 110, 662, 717
BspPI GGATC 2 cut(s) 422, 674
BspTNI GGTCTC 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 2 cut(s) 151, 325
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 424, 574, 711
BssMI GATC 5 cut(s) 132, 210, 225, 414, 679
BssSI CACGAG 2 cut(s) 202, 254
BssT1I CCWWGG 1 cut(s) 424
Bst2BI CACGAG 2 cut(s) 202, 254
Bst2UI CCWGG 2 cut(s) 113, 575
Bst4CI ACNGT 1 cut(s) 593
BstC8I GCNNGC 4 cut(s) 188, 281, 461, 711
BstDEI CTNAG 2 cut(s) 122, 398
BstDSI CCRYGG 1 cut(s) 711
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 5 cut(s) 234, 279, 405, 470, 713
BstHHI GCGC 3 cut(s) 279, 405, 674
BstKTI GATC 5 cut(s) 135, 213, 228, 417, 682
BstMAI GTCTC 2 cut(s) 66, 230
BstMBI GATC 5 cut(s) 132, 210, 225, 414, 679
BstMCI CGRYCG 2 cut(s) 36, 187
BstMWI GCNNNNNNNGC 3 cut(s) 274, 285, 579
BstNI CCWGG 2 cut(s) 113, 575
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 6 cut(s) 30, 87, 111, 449, 492, 573
BstUI CGCG 5 cut(s) 234, 279, 405, 470, 713
BstV1I GCAGC 2 cut(s) 449, 452
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 3 cut(s) 186, 543, 573
BtgI CCRYGG 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 1 cut(s) 459
Cac8I GCNNGC 4 cut(s) 188, 281, 461, 711
CfoI GCGC 3 cut(s) 279, 405, 674
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 4 cut(s) 270, 541, 571, 716
Cfr42I CCGCGG 1 cut(s) 714
CseI GACGC 1 cut(s) 272
Csp6I GTAC 1 cut(s) 328
CviAII CATG 2 cut(s) 460, 538
CviJI RGCY 7 cut(s) 126, 186, 543, 554, 568, 573, 635
CviKI_1 RGCY 7 cut(s) 126, 186, 543, 554, 568, 573, 635
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 2 cut(s) 122, 398
DpnI GATC 5 cut(s) 134, 212, 227, 416, 681
DpnII GATC 5 cut(s) 132, 210, 225, 414, 679
EaeI YGGCCR 1 cut(s) 184
EagI CGGCCG 1 cut(s) 184
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 270, 716
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 111, 573
EcoT14I CCWWGG 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 66
FaeI CATG 2 cut(s) 463, 541
FaiI YATR 2 cut(s) 461, 539
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 2 cut(s) 307, 661
FatI CATG 2 cut(s) 459, 537
FauI CCCGC 5 cut(s) 239, 422, 463, 706, 718
Fnu4HI GCNGC 6 cut(s) 406, 438, 441, 580, 583, 604
FokI GGATG 1 cut(s) 466
Fsp4HI GCNGC 6 cut(s) 406, 438, 441, 580, 583, 604
GlaI GCGC 3 cut(s) 278, 404, 673
GluI GCNGC 6 cut(s) 406, 438, 441, 580, 583, 604
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 2 cut(s) 401, 709
HaeIII GGCC 3 cut(s) 186, 543, 573
HapII CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 450, 493, 697
HgaI GACGC 1 cut(s) 272
HhaI GCGC 3 cut(s) 279, 405, 674
Hin1II CATG 2 cut(s) 463, 541
Hin6I GCGC 3 cut(s) 277, 403, 672
HinP1I GCGC 3 cut(s) 277, 403, 672
HinfI GANTC 5 cut(s) 59, 138, 179, 192, 320
HpaII CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 450, 493, 697
HphI GGTGA 2 cut(s) 135, 599
Hpy188I TCNGA 6 cut(s) 17, 64, 71, 123, 137, 399
Hpy188III TCNNGA 5 cut(s) 202, 262, 380, 630, 726
Hpy99I CGWCG 4 cut(s) 50, 288, 320, 346
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 1 cut(s) 593
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 1 cut(s) 508
HpyF10VI GCNNNNNNNGC 3 cut(s) 274, 285, 579
HpyF3I CTNAG 2 cut(s) 122, 398
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 2 cut(s) 463, 541
HspAI GCGC 3 cut(s) 277, 403, 672
KroI GCCGGC 1 cut(s) 186
KroNI GCCGGC 1 cut(s) 188
KspI CCGCGG 1 cut(s) 714
Kzo9I GATC 5 cut(s) 132, 210, 225, 414, 679
Lsp1109I GCAGC 2 cut(s) 449, 452
LweI GCATC 1 cut(s) 30
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 199, 587, 688
MalI GATC 5 cut(s) 134, 212, 227, 416, 681
MboI GATC 5 cut(s) 132, 210, 225, 414, 679
MboII GAAGA 1 cut(s) 142
MlyI GAGTC 2 cut(s) 68, 329
MmeI TCCRAC 1 cut(s) 42
MroNI GCCGGC 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 152
MseI TTAA 1 cut(s) 742
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 9 cut(s) 32, 89, 176, 183, 187, 289, 450, 493, 697
MspR9I CCNGG 6 cut(s) 32, 89, 113, 451, 494, 575
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 2 cut(s) 113, 575
MvnI CGCG 5 cut(s) 234, 279, 405, 470, 713
MwoI GCNNNNNNNGC 3 cut(s) 274, 285, 579
NaeI GCCGGC 1 cut(s) 188
NciI CCSGG 4 cut(s) 32, 89, 451, 494
NdeII GATC 5 cut(s) 132, 210, 225, 414, 679
NgoMIV GCCGGC 1 cut(s) 186
NlaIII CATG 2 cut(s) 463, 541
NlaIV GGNNCC 3 cut(s) 110, 662, 717
NmuCI GTSAC 3 cut(s) 199, 587, 688
NspI RCATGY 1 cut(s) 463
PaeI GCATGC 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 54
PctI GAATGC 1 cut(s) 391
PdiI GCCGGC 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 3 cut(s) 138, 179, 192
PfoI TCCNGGA 1 cut(s) 449
PkrI GCNGC 6 cut(s) 407, 439, 442, 581, 584, 605
PleI GAGTC 2 cut(s) 67, 328
PpsI GAGTC 2 cut(s) 67, 328
Psp6I CCWGG 2 cut(s) 111, 573
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 2 cut(s) 111, 573
PspN4I GGNNCC 3 cut(s) 110, 662, 717
PspPI GGNCC 4 cut(s) 270, 541, 571, 716
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SacII CCGCGG 1 cut(s) 714
SaqAI TTAA 1 cut(s) 742
SatI GCNGC 6 cut(s) 406, 438, 441, 580, 583, 604
Sau3AI GATC 5 cut(s) 132, 210, 225, 414, 679
Sau96I GGNCC 4 cut(s) 270, 541, 571, 716
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 2 cut(s) 68, 329
ScrFI CCNGG 6 cut(s) 32, 89, 113, 451, 494, 575
SetI ASST 7 cut(s) 78, 315, 601, 637, 657, 707, 740
SfaNI GCATC 1 cut(s) 30
Sfr303I CCGCGG 1 cut(s) 714
SgrBI CCGCGG 1 cut(s) 714
SinI GGWCC 2 cut(s) 270, 716
SphI GCATGC 1 cut(s) 463
StyD4I CCNGG 6 cut(s) 30, 87, 111, 449, 492, 573
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 1 cut(s) 593
TaiI ACGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 315, 480
TatI WGTACW 1 cut(s) 327
TauI GCSGC 4 cut(s) 408, 582, 585, 606
TfiI GAWTC 3 cut(s) 138, 179, 192
Tru1I TTAA 1 cut(s) 742
Tru9I TTAA 1 cut(s) 742
TseFI GTSAC 3 cut(s) 199, 587, 688
TseI GCWGC 2 cut(s) 437, 440
Tsp45I GTSAC 3 cut(s) 199, 587, 688
TspDTI ATGAA 2 cut(s) 154, 548
TspGWI ACGGA 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 270, 716
XceI RCATGY 1 cut(s) 463
XmnI GAANNNNTTC 1 cut(s) 152
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.