MD14G1038300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013698
Physical Location & Seq
Reverse (-)
3494218 .. 3494976
759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1038300.v1.1.491

Sequence Viewer

Length: 759 bp
ATGTCATCTCGCCGGAGACGGGAGAGAGTAGAGATGATGGAGGCCGAAGGAGGCGTCTCGTTCCCTGTCGTCTTCTCCGATGGCGAGACCGAGACCGATATCGGCGACCTCGTCGTTTACCCAAAGCTTGATTTCCCGCGGTTCCTGTCGGTTCTGAGCCACAAGATAGGAATCTTGCCGGGGCAGTTCACTGTTTTCCTATCCTCGCCGGAGACCCGCCAGCGAATCCCCATCACCGGCAAGGTCAACTTCAGCGCGATTTCTCAAGAGAAGAACTGCTGCTTCCTCGTGGAGCTCAAGCGGTCGAGGCGGCGGAAGAATCACAATTACCAGGTCCACCACCACCAAGACTTCCAAGAAGACGAGCACCACAACAAGGCGTCGTTGGGAGCTCAAAATCCGATCAACAAGAATCTCCATCGCGAGAATGTTATGCTCTTAAAACGCGGCATGGAGATCGAAAACGGAAACGCCGCCGGATTGACCATGCCTTTTGCGGGTCGGGCCGAGTACGAGAATGGGGCCAGGGAGCTGCAGTCTGAGAAAGAGAGGTACTTGACGGACATGGGGCTGGGACGGGCGGATGGGCTCGGGATTGGGCTGGGCCGCCGGAGTGGAGCGACGGCGTGCGAGGGGTGTTCGAGGGCGAACGAGATGGGGAGAGAAGTCGGGTTCCACTGGTGCGCTTACGACCCAGTGACTTTCGCGTTCAGGTCGCCGGCGGGTCCGATTTCCCGACCCGTTAAAGGGTCCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.25

Weight (kDa)

8.6

Isoelectric Point (pI)

60.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7138 PF23596 18 - 97 2.4e-36 Domain of unknown function (DUF7138)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20260
malus_domestica MD12G1039700.v1.1 MD14G1038300.v1.1
prunus_persica Prupe.7G095200_v2.0.a1
pyrus_communis pycom03g00540 pycom12g03370 pycom14g03320
rosa_chinensis RchiOBHm_Chr3g0475851
rosa_laevigata RLG00000023807
rosa_multiflora Rmu_co8277165.1_g000001
rosa_roxburghii Rroxscaffold_6G00405790
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3BG232000 Rh3CG226600 Rh3DG227200 Rh4CG218200 Rh7BG133300
rosa_wichuraiana Rw3G018360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 139, 257, 423, 447, 707
AcuI CTGAAG 1 cut(s) 235
AcyI GRCGYC 2 cut(s) 54, 380
AfaI GTAC 2 cut(s) 512, 554
AfiI CCNNNNNNNGG 6 cut(s) 19, 236, 376, 497, 746, 747
AjnI CCWGG 2 cut(s) 330, 524
AluBI AGCT 4 cut(s) 127, 295, 392, 532
AluI AGCT 4 cut(s) 127, 295, 392, 532
Alw21I GWGCWC 3 cut(s) 297, 369, 394
Alw26I GTCTC 5 cut(s) 10, 61, 80, 86, 206
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 4 cut(s) 42, 504, 522, 604
ApeKI GCWGC 2 cut(s) 279, 532
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspLEI GCGC 2 cut(s) 257, 686
AspS9I GGNCC 6 cut(s) 334, 504, 522, 604, 725, 750
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 3 cut(s) 334, 725, 750
BanII GRGCYC 3 cut(s) 297, 394, 591
BauI CACGAG 1 cut(s) 287
BbsI GAAGAC 2 cut(s) 64, 366
Bbv12I GWGCWC 3 cut(s) 297, 369, 394
BbvI GCAGC 2 cut(s) 266, 519
BccI CCATC 6 cut(s) 31, 74, 239, 426, 578, 649
BceAI ACGGC 1 cut(s) 639
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 2 cut(s) 332, 526
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 5 cut(s) 10, 61, 80, 86, 206
BfaI CTAG 1 cut(s) 757
BfmI CTRYAG 1 cut(s) 533
BisI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
BlsI GCNGC 6 cut(s) 281, 312, 449, 475, 534, 608
Bme1390I CCNGG 3 cut(s) 180, 332, 526
Bme18I GGWCC 3 cut(s) 334, 725, 750
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 6 cut(s) 334, 504, 522, 604, 725, 750
BmiI GGNNCC 5 cut(s) 143, 523, 674, 726, 751
BmrFI CCNGG 3 cut(s) 180, 332, 526
BmrI ACTGGG 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 689
BpiI GAAGAC 2 cut(s) 64, 366
BpuEI CTTGAG 2 cut(s) 249, 281
BpuMI CCSGG 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 170
BsaHI GRCGYC 2 cut(s) 54, 380
BsaI GGTCTC 3 cut(s) 80, 86, 206
BsaJI CCNNGG 3 cut(s) 137, 179, 525
BsaXI ACNNNNNCTCC 2 cut(s) 521, 551
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 236, 376, 497, 746, 747
Bse118I RCCGGY 2 cut(s) 236, 718
Bse1I ACTGG 2 cut(s) 683, 695
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 2 cut(s) 332, 526
BseDI CCNNGG 3 cut(s) 137, 179, 525
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 6 cut(s) 19, 236, 376, 497, 746, 747
BseMII CTCAG 2 cut(s) 146, 531
BseNI ACTGG 2 cut(s) 683, 695
BseXI GCAGC 2 cut(s) 266, 519
BseYI CCCAGC 2 cut(s) 571, 601
Bsh1236I CGCG 5 cut(s) 139, 257, 423, 447, 707
Bsh1285I CGRYCG 1 cut(s) 305
BshFI GGCC 4 cut(s) 44, 506, 524, 606
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 3 cut(s) 297, 369, 394
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 7 cut(s) 13, 179, 209, 237, 477, 610, 719
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 6 cut(s) 19, 236, 376, 497, 746, 747
BsmAI GTCTC 5 cut(s) 10, 61, 80, 86, 206
BsmBI CGTCTC 2 cut(s) 10, 61
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 4 cut(s) 44, 506, 524, 606
Bso31I GGTCTC 3 cut(s) 80, 86, 206
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 4 cut(s) 297, 369, 394, 591
Bsp143I GATC 2 cut(s) 402, 456
Bsp68I TCGCGA 1 cut(s) 423
BspANI GGCC 4 cut(s) 44, 506, 524, 606
BspCNI CTCAG 2 cut(s) 147, 532
BspFNI CGCG 5 cut(s) 139, 257, 423, 447, 707
BspLI GGNNCC 5 cut(s) 143, 523, 674, 726, 751
BspMAI CTGCAG 1 cut(s) 537
BspTNI GGTCTC 3 cut(s) 80, 86, 206
BsrFI RCCGGY 2 cut(s) 236, 718
BsrI ACTGG 2 cut(s) 683, 695
BssAI RCCGGY 2 cut(s) 236, 718
BssECI CCNNGG 3 cut(s) 137, 179, 525
BssMI GATC 2 cut(s) 402, 456
BssNI GRCGYC 2 cut(s) 54, 380
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 332, 526
Bst4CI ACNGT 1 cut(s) 193
BstACI GRCGYC 2 cut(s) 54, 380
BstC8I GCNNGC 3 cut(s) 221, 628, 720
BstDEI CTNAG 2 cut(s) 155, 540
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 589
BstFNI CGCG 5 cut(s) 139, 257, 423, 447, 707
BstHHI GCGC 2 cut(s) 257, 686
BstKTI GATC 2 cut(s) 405, 459
BstMAI GTCTC 5 cut(s) 10, 61, 80, 86, 206
BstMBI GATC 2 cut(s) 402, 456
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 2 cut(s) 307, 503
BstNI CCWGG 2 cut(s) 332, 526
BstSCI CCNGG 3 cut(s) 178, 330, 524
BstSFI CTRYAG 1 cut(s) 533
BstUI CGCG 5 cut(s) 139, 257, 423, 447, 707
BstV1I GCAGC 2 cut(s) 266, 519
BstV2I GAAGAC 2 cut(s) 64, 366
BsuRI GGCC 4 cut(s) 44, 506, 524, 606
BtgI CCRYGG 1 cut(s) 137
BtgZI GCGATG 1 cut(s) 404
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 3 cut(s) 189, 676, 702
BtuMI TCGCGA 1 cut(s) 423
Cac8I GCNNGC 3 cut(s) 221, 628, 720
CfoI GCGC 2 cut(s) 257, 686
Cfr10I RCCGGY 2 cut(s) 236, 718
Cfr13I GGNCC 6 cut(s) 334, 504, 522, 604, 725, 750
Cfr42I CCGCGG 1 cut(s) 140
CseI GACGC 2 cut(s) 43, 369
CsiI ACCWGGT 1 cut(s) 330
Csp6I GTAC 2 cut(s) 511, 553
CviAII CATG 3 cut(s) 451, 487, 565
CviQI GTAC 2 cut(s) 511, 553
DdeI CTNAG 2 cut(s) 155, 540
DpnI GATC 2 cut(s) 404, 458
DpnII GATC 2 cut(s) 402, 456
EciI GGCGGA 2 cut(s) 328, 596
Ecl136II GAGCTC 2 cut(s) 295, 392
Eco24I GRGCYC 3 cut(s) 297, 394, 591
Eco31I GGTCTC 3 cut(s) 80, 86, 206
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 3 cut(s) 334, 725, 750
Eco53kI GAGCTC 2 cut(s) 295, 392
Eco57I CTGAAG 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 590
EcoICRI GAGCTC 2 cut(s) 295, 392
EcoRII CCWGG 2 cut(s) 330, 524
EcoRV GATATC 1 cut(s) 100
EcoT38I GRGCYC 3 cut(s) 297, 394, 591
Esp3I CGTCTC 2 cut(s) 10, 61
FaeI CATG 3 cut(s) 454, 490, 568
FaiI YATR 4 cut(s) 434, 452, 488, 566
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 588
FatI CATG 3 cut(s) 450, 486, 564
FauI CCCGC 4 cut(s) 144, 224, 490, 715
Fnu4HI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 3 cut(s) 297, 394, 591
Fsp4HI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
FspBI CTAG 1 cut(s) 757
GlaI GCGC 2 cut(s) 256, 685
GluI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
GsaI CCCAGC 2 cut(s) 575, 605
HaeIII GGCC 4 cut(s) 44, 506, 524, 606
HapII CCGG 7 cut(s) 13, 179, 209, 237, 477, 610, 719
HgaI GACGC 2 cut(s) 43, 369
HhaI GCGC 2 cut(s) 257, 686
Hin1I GRCGYC 2 cut(s) 54, 380
Hin1II CATG 3 cut(s) 454, 490, 568
Hin6I GCGC 2 cut(s) 255, 684
HinP1I GCGC 2 cut(s) 255, 684
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 4 cut(s) 171, 225, 319, 412
HpaII CCGG 7 cut(s) 13, 179, 209, 237, 477, 610, 719
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 4 cut(s) 118, 189, 247, 337
Hpy188I TCNGA 6 cut(s) 79, 156, 402, 541, 729, 754
Hpy188III TCNNGA 4 cut(s) 266, 422, 592, 735
Hpy8I GTNNAC 4 cut(s) 118, 189, 247, 337
Hpy99I CGWCG 3 cut(s) 116, 385, 625
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4V TGCA 1 cut(s) 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 503
HpyF3I CTNAG 2 cut(s) 155, 540
Hsp92I GRCGYC 2 cut(s) 54, 380
Hsp92II CATG 3 cut(s) 454, 490, 568
HspAI GCGC 2 cut(s) 255, 684
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
KspI CCGCGG 1 cut(s) 140
Kzo9I GATC 2 cut(s) 402, 456
LmnI GCTCC 4 cut(s) 292, 389, 529, 617
Lsp1109I GCAGC 2 cut(s) 266, 519
MabI ACCWGGT 1 cut(s) 330
MaeI CTAG 1 cut(s) 757
MaeIII GTNAC 1 cut(s) 697
MalI GATC 2 cut(s) 404, 458
MboI GATC 2 cut(s) 402, 456
MboII GAAGA 4 cut(s) 64, 283, 328, 371
MhlI GDGCHC 4 cut(s) 297, 369, 394, 591
MluCI AATT 1 cut(s) 325
MnlI CCTC 9 cut(s) 34, 44, 119, 214, 296, 300, 543, 625, 636
MreI CGCCGGCG 1 cut(s) 718
MroNI GCCGGC 1 cut(s) 718
MseI TTAA 2 cut(s) 440, 744
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 7 cut(s) 13, 179, 209, 237, 477, 610, 719
MspR9I CCNGG 3 cut(s) 180, 332, 526
MvaI CCWGG 2 cut(s) 332, 526
MvnI CGCG 5 cut(s) 139, 257, 423, 447, 707
MwoI GCNNNNNNNGC 2 cut(s) 307, 503
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 1 cut(s) 180
NdeII GATC 2 cut(s) 402, 456
NgoMIV GCCGGC 1 cut(s) 718
NlaIII CATG 3 cut(s) 454, 490, 568
NlaIV GGNNCC 5 cut(s) 143, 523, 674, 726, 751
NmeAIII GCCGAG 1 cut(s) 532
NmuCI GTSAC 1 cut(s) 697
NruI TCGCGA 1 cut(s) 423
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 108
PdiI GCCGGC 1 cut(s) 720
PfeI GAWTC 4 cut(s) 171, 225, 319, 412
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 6 cut(s) 281, 312, 449, 475, 534, 608
Psp124BI GAGCTC 2 cut(s) 297, 394
Psp6I CCWGG 2 cut(s) 330, 524
PspFI CCCAGC 2 cut(s) 571, 601
PspGI CCWGG 2 cut(s) 330, 524
PspN4I GGNNCC 5 cut(s) 143, 523, 674, 726, 751
PspPI GGNCC 6 cut(s) 334, 504, 522, 604, 725, 750
PstI CTGCAG 1 cut(s) 537
PsyI GACNNNGTC 1 cut(s) 110
RruI TCGCGA 1 cut(s) 423
RsaI GTAC 2 cut(s) 512, 554
RsaNI GTAC 2 cut(s) 511, 553
SacI GAGCTC 2 cut(s) 297, 394
SacII CCGCGG 1 cut(s) 140
SaqAI TTAA 2 cut(s) 440, 744
SatI GCNGC 6 cut(s) 280, 311, 448, 474, 533, 607
Sau3AI GATC 2 cut(s) 402, 456
Sau96I GGNCC 6 cut(s) 334, 504, 522, 604, 725, 750
ScrFI CCNGG 3 cut(s) 180, 332, 526
SduI GDGCHC 4 cut(s) 297, 369, 394, 591
SetI ASST 9 cut(s) 111, 129, 246, 297, 336, 394, 534, 554, 716
SexAI ACCWGGT 1 cut(s) 330
SfcI CTRYAG 1 cut(s) 533
Sfr303I CCGCGG 1 cut(s) 140
SgrAI CRCCGGYG 1 cut(s) 718
SgrBI CCGCGG 1 cut(s) 140
SinI GGWCC 3 cut(s) 334, 725, 750
SmlI CTYRAG 2 cut(s) 264, 296
SmoI CTYRAG 2 cut(s) 264, 296
Sse9I AATT 1 cut(s) 325
SspMI CTAG 1 cut(s) 757
SstI GAGCTC 2 cut(s) 297, 394
StyD4I CCNGG 3 cut(s) 178, 330, 524
TaaI ACNGT 1 cut(s) 193
TaqI TCGA 3 cut(s) 305, 459, 641
TaqII GACCGA 2 cut(s) 104, 110
TasI AATT 1 cut(s) 325
TauI GCSGC 4 cut(s) 313, 450, 476, 609
TfiI GAWTC 4 cut(s) 171, 225, 319, 412
Tru1I TTAA 2 cut(s) 440, 744
Tru9I TTAA 2 cut(s) 440, 744
TscAI CASTG 3 cut(s) 196, 683, 702
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 2 cut(s) 279, 532
Tsp45I GTSAC 1 cut(s) 697
TspGWI ACGGA 2 cut(s) 480, 575
TspRI CASTG 3 cut(s) 196, 683, 702
Tth111I GACNNNGTC 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 334, 725, 750
XspI CTAG 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.